KR20140068437A

KR20140068437A - Spuit

Info

Publication number: KR20140068437A
Application number: KR20120135927A
Authority: KR
Inventors: 이용석; 송근국; 이효근; 박효림; 최형길
Original assignee: 에스디 바이오센서 주식회사
Priority date: 2012-11-28
Filing date: 2012-11-28
Publication date: 2014-06-09
Also published as: WO2014084509A1; KR101553041B1

Abstract

The present invention relates to a spuit having a structure and components for mixing solutions. More specifically, the present invention relates to a spuit that includes a boronic acid latex ball in an inflow pipe and has a through-hole in a cap unit so that a pre-treatment process can be performed in which a blood sample, a buffer solution, and the boronic acid latex ball are mixed when glycated hemoglobin (HbA1c) is measured through the spuit. In the spuit of the present invention, the cap unit made of elastic material is coupled with the top end of the inflow pipe through which solutions are fed. The through-hole(s) is/are formed on both left and right sides or on one side of the cap unit. The inflow pipe comprises: a tip unit that is disposed at the bottom end of the inflow pipe and formed in a pipe structure; an inflow pipe bottom in which the tip unit is inserted; and an inflow pipe top that is disposed at the top of the inflow pipe bottom and has a step pulley on the inflow pipe bottom. A protruding unit that is formed by the tip unit inserted in the inflow pipe bottom in order to protrude is included inside the inflow pipe bottom. Moreover, a latex ball is prepared in the inflow pipe bottom. A ring for preventing the movement of a ball is fixed to and mounted on the lower part of the inflow pipe top. The latex ball includes boronic acid.

Description

Sighting {Spuit}

The present invention relates to a syringe having a structure and components for solution mixing. More specifically, the present invention relates to a syringe having a boronic acid latex ball in an inlet tube and a through hole in a cap portion, The present invention relates to a syringe capable of performing a pretreatment process of mixing a blood sample, a buffer solution and a boronic acid latex ball when measuring glycated hemoglobin (HbA1c).

Glycated hemoglobin (HbA1c) is a type of hemoglobin used to detect blood glucose (blood sugar) concentration over a long period of time, and it reflects the recent 2 to 3 month blood glucose level. The glycated hemoglobin can be detected by the blood test. It can be used to determine whether the diabetic control is performed well at the time of follow-up.

In the conventional measurement of glycation hemoglobin, the collected blood is put into a reaction reagent, mixed well and allowed to stand for 2 minutes. The reaction solution mixture in which the reagent and blood have reacted is dispensed into a test strip and the washing solution (buffer solution) Then, the test strip is attached to the saccharide blood measuring device and analyzed.

FIG. 1 is an example of a conventional pretreatment apparatus for measuring saccharification blood, and FIG. 2 is a state of use of a pretreatment apparatus for measuring saccharification blood of FIG.

FIG. 1 shows a conventional apparatus for measuring saccharified blood, which comprises a first pipette 310 for taking 5 ul of blood sample and putting it into a mixing tube 300, and a 200 μl buffer solution 330 in a mixing tube 300 The mixed solution is mixed well in the mixing tube 300 and then introduced into the sample inlet of the test strip 350. The test strip 350 is then passed through the saccharified blood measurement device To measure the saccharide blood.

That is, the process of dispensing the collected blood to the test strip by mixing the reagent solution and the buffer solution well mixed with each other is cumbersome and complicated. In addition, two pipettes (for 200ul and 5ul) and a pipette tip for each pipette were required, and it was troublesome to insert and pull the pipette tip into and out of the pipette, and to open and close the lid of the buffer vessel. Therefore, a device for a simplified process is required.

To this end, the present invention proposes an eyedropper having a built-in eyedrop latex ball and capable of performing pipetting and mixing. The syringe of the present invention can be subjected to a pretreatment process in which the buffer and the buffer solution are mixed well with the reaction reagent in the blood as a single device.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a method for measuring hemoglobin (HbA1c), which comprises a Boronic Acid Latex Ball and a tip integrally therewith, The present invention provides a syringe capable of performing a pretreatment process of mixing a boronic acid latex ball.

Another problem to be solved by the present invention is to provide a cap structure that has a tube structure for sucking a predetermined amount of blood samples by capillary phenomenon and has a through hole on both sides of the cap portion 100 to suck blood by capillary phenomenon Is to provide a syringe.

Another problem to be solved by the present invention is to provide a syringe which has been subjected to a hydrophilic treatment so that the inside of the tip portion of the syringe sucks blood well, and to which heparin treatment is applied to prevent the blood from being sucked.

Another object of the present invention is to provide a syringe having a structure and components for solution mixing.

Another problem to be addressed by the present invention is the incorporation of a bronze latex ball which is adapted to be mixed with a 200 ul buffer and a 5 ul blood sample with a syringe having a ring to prevent the bronze latex ball from moving to the top of the syringe .

According to an aspect of the present invention, there is provided an eyedropper having a cap portion made of an elastic material coupled to an upper end of an inflow tube portion through which a solution flows, the eyedropper having a through hole on one or both sides of the cap portion, A tip portion positioned at a lower end of the inflow tube portion and having a tube structure; A bottom portion of the inflow pipe where the tip portion is inserted, an upper portion of the inflow pipe which is located on the lower portion of the inflow pipe, and which forms a step with the lower portion of the inflow pipe, And a protruding portion formed on the protruding portion.

A latex ball is embedded in the lower portion of the inflow pipe, and a ring for preventing ball movement is fixedly mounted on a bottom portion of the inflow pipe. The latex ball includes bromonic acid.

According to another aspect of the present invention, there is provided an eyedropper having a cap portion made of an elastic material coupled to an upper end of an inflow tube portion through which a solution flows, the inflow tube portion including: a tip portion positioned at a lower end of the inflow tube portion; A lower portion of the inflow pipe into which the tip portion is inserted; And an upper portion of the inflow pipe which is located above the lower portion of the inflow pipe and has a stepped portion with the lower portion of the inflow pipe, wherein a latex ball containing Bronno acid is built in the lower portion of the inflow pipe, And the prevention ring is fixedly mounted.

The lower end of the inflow pipe has a smaller inner diameter as it approaches the tip, and the diameter of the bronze latex ball is larger than the inner diameter of the lower end of the inflow pipe and smaller than the upper inner diameter of the lower portion of the inflow pipe.

Both sides of the cap portion are provided with recesses, and the recesses are located in the recesses.

The inside of the tip portion is subjected to a hydrophilic treatment and a heparin treatment.

The inner diameter of the ball prevention ring is smaller than the diameter of the boronic acid latex ball.

A tongue is provided at a lower portion of a side portion of the ball restraining ring, and the tongue is engaged with a groove provided in an inner lower portion of the upper portion of the inflow pipe.

The cap portion is made of a rubber material, a silicon material, or a PE (plastic) material. The inflow pipe portion is made of a plastic material or a glass material. The ring for preventing ball movement is made of rubber material, silicone material, or PE (plastic) material.

The syringe is for separating glycated hemoglobin. When the syringe is sucked with the syringe and then the buffer solution is mixed with the syringe, the syringe hemoglobin (HbA1c) contained in the blood, Of glucose to separate glycosylated hemoglobin from common non-glycosylated hemoglobin (Hb).

When the blood is sucked into the eyedropper, the opening of the cap part is opened so as to be sucked.

When the buffer solution is put into the syringe and mixed, the syringe containing the blood is placed in a buffer solution tube containing the buffer solution, and the cap part is closed with the through hole of the cap part being closed, whereby blood, A latex ball, and a buffer solution are mixed.

According to the eyedropper of the present invention, a boric acid latex ball is provided and a tip is integrally provided to perform pre-treatment in which 5ul blood sample, 200ul buffer and boronic acid latex ball are mixed with one syringe at the time of measuring glycated hemoglobin (HbA1c) Process can be performed.

In addition, the eyedropper of the present invention has a tubular structure for sucking a predetermined amount of blood sample by capillary phenomenon, and the cap portion 100 has a through hole on both sides thereof to suck blood by capillary phenomenon .

In addition, in the eyedropper of the present invention, the inside of the tip portion of the eyedropper is subjected to hydrophilic treatment so as to suck blood well, and heparin treatment is performed so as to prevent the coagulation of the drawn blood, It is made to not be.

Further, the eyedropper of the present invention has a structure and components for solution mixing, is easy to use, economical, and disposable, and is sanitary and safe.

In addition, the syringe of the present invention incorporates a bronze latex ball, which is made to mix with a 200 ul buffer and a 5 ul blood sample, with a ring to prevent the bronze latex ball from moving to the top of the syringe, So that the ball moves to the cap part and does not hamper the pre-processing.

In the conventional pretreatment method, two pipettes (for 200ul and 5ul) and a pipette tip for each pipette were required, and it was troublesome to insert the pipette tip into and out of the pipette and to open and close the buffer vessel lid However, in the present invention, it is possible to perform preprocessing with one eyedropper.

In the present invention, in the pretreatment for the measurement of glycated hemoglobin (% HbA1c), the through hole 110 of the cap part 100 is opened in the dropper 10 and 5 ul of the blood is added. The buffer solution is introduced into the buffer solution tube 305 and the solution is mixed in the dropper by pressing the cap part 100 which covers the through hole 110.

Particularly, the eyedropper of the present invention is used in a single use and has an advantage that a user can use the eyedropper conveniently and safely.

In addition, the syringe of the present invention can be used for pretreatment for various diagnostic fields such as Cardiac, Infection, Rheumatoid Arthritis, Hormone, and Cancer as well as a pretreatment process for analyzing HbA1c. Of the tip is not limited to blood, but can also be applied to urine collection, such as the Urine Capillary Tip.

1 is an example of a conventional pretreatment apparatus for measuring saccharide blood.
FIG. 2 is a use state diagram of the pretreatment apparatus for measuring saccharification blood of FIG. 1; FIG.
3 is a perspective view of the eyedropper of the present invention.
Fig. 4 is an explanatory diagram for explaining the inside of the eyedropper of Fig. 3; Fig.
5 is an enlarged view of the cap portion of Fig.
FIG. 6 is an explanatory diagram for explaining a cross section of the cap portion of FIG. 4;
Fig. 7 is an enlarged view of the ball restraining ring of Fig. 4;
Figure 8 is a cross-sectional view of the tip of Figure 4;
9 is a pre-treatment device for measuring glycated hemoglobin using the eyedrop of the present invention.
Fig. 10 is an explanatory view for explaining a method of using the eyedropper of the present invention. Fig.

Hereinafter, the eyedropper of the present invention will be described in detail with reference to the attached drawings.

Fig. 3 is a perspective view of the eyedropper of the present invention, and Fig. 4 is an explanatory view for explaining the inside of the eyedropper of Fig.

The eyedropper 10 comprises a cap part 100 and an inflow tube part 200.

The cap part 100 is a circular cap made of a rubber material or a silicone material and forms a negative pressure. The cap part 100 has a through hole 110 on both left and right sides thereof and has a through hole 110. In order to serve as a general pipette, a force is applied to the cap portion 100 while the through hole 110 is closed with a finger, thereby generating a negative pressure so that the liquid may flow in or out.

The cap part 100 is preferably made of a silicone material. However, it is possible to use PE (plastic material) having elasticity in addition to all rubber materials. When the cap part 100 is made of a PE material, It is possible to use.

The through holes 110 can be filled with the liquid by opening and closing the through holes with the fingers when mixing the different liquids introduced into the eyedropper 10. The through hole 110 is located inside the circular recess 120. The through hole 110 is a means for applying a negative pressure to the liquid surface, and causes the liquid to move up or down along the inflow tube 200 according to the negative pressure applied to the liquid surface by the principle of capillary action and siphon.

The through hole may exist only on one side of the cap part 100. [

The circular concave portion 120 is a portion where a finger is stuck, and serves to prevent the eyedropper 10 from falling off from the finger when the hole is opened and closed with a finger.

The inflow tube 200 is a cylindrical inflow tube made of transparent or translucent plastic material and has a shape gradually tapering toward the lower end and includes a tip portion 210, an inflow tube bottom 240, and an inflow tube top 270 . When the cap part 100 is pressed once with one end of the tip part 210 inserted into a container containing the solution, a proper amount of solution flows into the tip part 210.

The tip portion 210 is located at the lower end of the inflow tube portion 200 and is made of a tube thinner than the tube of the lower portion of the inflow tube 240 and the upper portion of the inflow tube portion 270. The tip portion 210 is made of a plastic material in a blood capillary type and has a hydrophilic treatment and a heparin (blood coagulation prevention) treatment inside the tip portion 210 to facilitate sucking of a blood sample. That is, the inside of the tip portion 210 is subjected to a hydrophilic treatment so as to suck blood well, and heparin treatment is performed to prevent the blood from being solidified. The tip portion 210 is made capable of blood collection.

The inflow tube 200 of the eyedropper can be made of a transparent or semitransparent plastic material, but a glass material can also be used.

In addition, the tip portion 210 has a tubular structure in order to utilize the capillary phenomenon to suck blood, and the upper portion of the tip portion 210 is inserted and protruded into the lower portion of the inflow tube 240. However, in order to collect only 5 ul of the blood sample, the height of the protrusion was not so high but slightly protruded.

The inflow tube lower part 240 is located between the inflow tube upper part 270 and the tip part 210 and forms a step with the inflow tube upper part 270 to form a step with the tip part 210, The inner diameter of the inflow pipe upper part 270 is larger than that of the tip part 210. The inner diameter of the inflow pipe upper part 270 is increased as the inflow pipe upper part 270 is closer to the inflow pipe upper part 270, A boron acid latex ball 250 is contained in the lower portion of the inflow pipe 240.

The radius of the boronic acid latex ball 250 is larger than the inner diameter of the middle portion of the inflow tube bottom 240 so as to extend over the middle portion of the inflow tube bottom 240. The main raw material, boronic acid, binds specifically to glucose of HbA1c and separates HbA1c from normal HbA.

The boronate latex ball 250 is located on the inner surface of the middle portion of the lower portion of the inlet pipe 240 and the lower portion of the inlet pipe 240 is divided into upper and lower portions by the boronic acid latex ball 250, The air pressure at the upper portion and the lower portion of the boronic acid latex ball 250 at the lower portion 240 are different from each other.

In the present invention, boron acid latex balls are exemplified for convenience of explanation, but the present invention is not limited thereto. The main components of the latex balls are not limited to boronic acid and may be changed according to the field to be inspected .

When the cap part 100 is pressed and compressed while the tip part 210 is immersed in the solution without applying force to the cap part 100 and the through hole 110 of the cap part 100 is closed, The air filled in the upper part of the boronic acid latex ball 250 pushes down the boronic acid latex ball 250. At this time, the boronic acid latex ball 250 is lowered, . When the cap portion 100 is elastically restored and the air that has been on the upper portion of the boronic acid latex ball 250 moves toward the cap portion 100, the boronic acid latex So that the ball 250 is lifted and lowered. At this time, when the force for pressing the cap part 100 is released, more air flows into the upper part of the boronic acid latex ball 250 when the hole is opened to the through hole 110. Therefore, the boronic acid latex ball 250) moves toward the cap portion (100). By doing so, the specific binding of glucose to the glucose of the glycated hemoglobin (HbA1c) in the solution (for example, blood) in the inflow tube bottom 240 and the glycosylated hemoglobin (HbA1c) (Hb).

The upper portion of the inflow pipe 270 has a cap portion 100 and the lower portion of the inflow pipe 240 is positioned below the inflow pipe upper portion 270. The inner diameter of the inflow pipe upper portion 270 is larger than the inner diameter of the inflow pipe lower portion 240, It accomplishes.

A ball restraining ring 280 is mounted on the inner side of the lower portion of the inflow pipe upper portion 270 and the inflow pipe lower portion 240, more specifically, the lower portion of the inflow pipe upper portion 270, .

The inner diameter of the ball prevention ring 280 is made smaller than the diameter of the boronic acid latex ball 250 so that the ball migration prevention ring 280 prevents the boronic acid latex ball 250 from flowing into the upper pipe 270 prevent. The ball restraining ring 280 is made of a rubber material, a silicon material, or a plastic material.

The upper portion of the inflow pipe 270 and the lower portion of the inflow pipe 240 are integrally formed. In some cases, the tip portion 210, the upper portion of the inflow pipe 270 and the lower portion of the inflow pipe 240 may be integrally formed.

FIG. 5 is an enlarged view of the cap portion of FIG. 4, and FIG. 6 is an explanatory view for explaining a cross section of the cap portion of FIG.

The cap portion 100 is provided with recesses 120 on both sides and the through hole 110 is located in the recess portion 120.

Fig. 7 is an enlarged view of the ball restraining ring of Fig. 4;

The inner diameter of the ball movement preventing ring 280 is smaller than the diameter of the boronic acid latex ball 250. A circular protrusion 282 is formed at a lower portion of the circumference of the ring 280 Respectively. The jaw 282 is engaged with the groove provided in the lower inner portion of the inlet pipe upper portion 270 and mounted on the lower inner side of the inlet pipe upper portion 270.

Figure 8 is a cross-sectional view of the tip of Figure 4;

The tip portion 210 is mounted to the inflow tube lower portion 240 and has an inner diameter smaller than the inner diameter of the inflow tube lower portion 240. The tip portion 210 has a tubular structure to utilize the capillary phenomenon.

The following describes the method of manufacturing the eyedropper of the present invention.

The inside of the tip portion 210 is subjected to a hydrophilic treatment and a heparin treatment.

The boron acid latex ball 250 is inserted into the inflow pipe 200 and the ball blocking prevention ring 280 is attached to the lower portion of the inflow pipe upper portion 270.

Next, the cap portion 100 is mounted on the upper portion of the inflow tube portion 200.

FIG. 9 is a pre-treatment device for measuring glycated hemoglobin using the eyedropper of the present invention, and FIG. 10 is an explanatory view for explaining a method of using the eyedropper of the present invention.

As shown in FIG. 10 (a), 5 ul of blood is taken from the punctured fingertip, and the through hole 110, which is blocked before sampling, is opened and collected. The inside of the tip portion 210 is subjected to a hydrophilic treatment and a heparin treatment, and by the through hole 110 of the cap portion 100, 5ul is sucked into the eyedropper by capillary phenomenon.

10 (b), the capillary 100 is pressed or uncompressed in a state where the eyedropper is inserted into the buffer solution tube 305 containing 200 ul of the buffer solution and both the through holes 110 are covered with fingers (About 10 times). At this time, a mixed solution of blood, a bronate latex ball 250 and a buffer solution is contained in the syringe and buffer solution tube.

As shown in FIG. 10C, after the entire solution mixed from the buffer solution tube is sucked into the syringe, the solution is slowly dropped to the test strip 350.

In the pretreatment for the measurement of glycated hemoglobin (% HbA1c), 5 l of the blood collected in the eyedropper of the present invention, which is a syringe containing the boronic acid latex ball 250, , And the solution is mixed in the dropper using the through hole (110).

In the conventional method, two pipettes (for 200ul and 5ul) and a pipette tip for each pipette were required, and it was troublesome to insert and pull the pipette tip into and out of the pipette, and to open and close the lid of the buffer vessel .

Particularly, the present invention can easily collect blood 5 ul by the tip 210 and the vent hole 110, which are blood capillary tips, and that the boronic acid latex ball 250 exists inside the inlet tube 200 Therefore, after collecting the blood, the tip 210 can be inserted into the buffer tube, and the solution can be easily mixed in the form of an eyedropper (a method of pressing or depressurizing the cap portion 100 of the eyedropper). This can shorten the preprocessing time.

The eyedropper of the present invention is used in a single use, which is advantageous in that the eyedropper can be conveniently and safely used by the user.

Unlike the conventional eyedropper, the eyedropper of the present invention has a tubular structure for sucking a predetermined amount of blood sample into a capillary phenomenon and has a through hole on both sides of the cap portion 100.

The eyedropper of the present invention is used in a state in which the through hole 110 is not closed when collecting a blood sample, and both the through holes 110 are naturally sealed with fingers when the solution is mixed. The cap portion 100 is provided with a recess 120 so that the user can easily seal or open the through-hole 110.

The inside of the tip portion 210 of the eyedropper of the present invention is subjected to a hydrophilic treatment so as to suck the blood well, and heparin treatment is performed to prevent the blood from being solidified.

The tip portion 210 of the eyedropper of the present invention is mounted on the inflow tube lower portion 240 of the inflow tube 200 so that the upper portion of the tip portion 210 is moved upward in the inflow tube lower portion 240, ). In order to collect only 5 ul of the blood sample, the height of the protruding portion was not high but slightly protruded.

The eyedropper of the present invention incorporates a bronze latex ball, which is configured to be mixed with a 200 ul buffer and a 5 ul blood sample, and has a ring that prevents the bronze latex ball from moving to the top of the eyedropper.

10: Syringe 100: Cap
110: through hole 120: circular recess
200: inlet tube 210: tip
240: inlet pipe bottom 250: boron acid latex balls
270: inlet pipe top 280: ball restraining ring
282: jaw 350: test strip

Claims

And a cap portion made of an elastic material is coupled to an upper end of the inflow pipe portion into which the solution flows,
And a through hole is provided on both sides or one side of the right and left sides of the cap portion,
The inflow pipe portion
A tip portion positioned at a lower end of the inflow tube portion and having a tube structure;
A lower portion of the inflow pipe into which the tip portion is inserted;
And an upper portion of the inflow pipe located above the inflow pipe, the inflow pipe being stepped with the lower portion of the inflow pipe,
And a protruding portion inserted into the lower portion of the inflow pipe to protrude in the lower portion of the lower portion of the inflow pipe.

The method of claim 1, wherein
A latex ball is embedded in the lower part of the inflow pipe,
And a ball restraining ring is fixedly mounted on a bottom portion of the upper portion of the inflow pipe.

3. The method of claim 2,
Characterized in that the latex ball comprises brononic acid.

And a cap portion made of an elastic material is coupled to an upper end of the inflow pipe portion into which the solution flows,
The inflow pipe portion
A tip portion positioned at a lower end of the inflow tube portion and having a tube structure;
A lower portion of the inflow pipe into which the tip portion is inserted;
And an upper portion of the inflow pipe located above the inflow pipe, the inflow pipe being stepped with the lower portion of the inflow pipe,
A latex ball containing brononic acid is embedded in the lower part of the inflow pipe,
And a ball restraining ring is fixedly mounted on a bottom portion of the upper portion of the inflow pipe.

5. The method according to any one of claims 2 to 4,
The lower part of the inflow pipe has a smaller inner diameter as it approaches the tip,
Wherein the diameter of the bronze latex ball is larger than the inner diameter of the lower end of the lower portion of the inflow pipe and smaller than the upper inner diameter of the lower portion of the inflow pipe.

4. The method according to any one of claims 1 to 3,
The cap portion is provided with recesses on the left and right sides thereof,
And the through hole is positioned in the recess.

4. The method according to any one of claims 1 to 3,
And the inside of the tip portion is subjected to a hydrophilic treatment and a heparin treatment.

5. The method according to any one of claims 2 to 4,
Wherein the ring for preventing ball movement has an inner diameter smaller than the diameter of the boronic acid latex ball.

9. The method of claim 8,
And a jaw provided at a lower portion of a side portion of the ball blocking prevention ring, the jaw being engaged with a groove provided in an inner lower portion of the upper portion of the inflow pipe.

5. The method according to any one of claims 1 to 4,
Wherein the cap portion is made of a rubber material, a silicone material, or a PE (plastic) material.

5. The method according to any one of claims 1 to 4,
Wherein the inflow pipe portion is made of a plastic material or a glass material.

5. The method according to any one of claims 2 to 4,
Characterized in that the ring for preventing ball movement is made of rubber material, silicone material or PE (plastic) material.

5. The method according to any one of claims 3 to 4,
Wherein said syringe is for separating glycated hemoglobin.

14. The method of claim 13,
When the syringe is aspirated with the syringe and then the buffer solution is mixed with the syringe, the Brona acid contained in the latex ball binds specifically to the glucose of the glycated hemoglobin (HbA1c) contained in the blood, Wherein the hemoglobin is separated from common non-glycosylated hemoglobin (Hb).

15. The method of claim 14,
And when the blood is sucked by the syringe, the syringe is sucked in a state that the opening of the cap is opened.

16. The method of claim 15,
When the buffer solution is put into the syringe and mixed, the syringe containing the blood is placed in a buffer solution tube containing the buffer solution, and the cap part is closed with the through hole of the cap part being closed, whereby blood, A latex ball, and a buffer solution.