CN109781478B - Integrated automatic pretreatment device for high-flux chromatography detection - Google Patents
Integrated automatic pretreatment device for high-flux chromatography detection Download PDFInfo
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Abstract
The invention discloses an integrated automatic pretreatment device for high-throughput chromatography detection, aiming at integrating chemical reaction, uniform mixing, centrifugation, incubation and sample introduction in pretreatment of dairy products into an automatic device, improving the pretreatment efficiency of the dairy products to be detected and realizing 'one-stop' pretreatment. The device comprises an integrated centrifuge, a plunger pump, a three-way valve and a multi-channel incubation-sample introduction device. The plunger pump provides power for the whole pretreatment device, so that the drawing of a target solution treated in the integrated centrifuge, the filling of an incubation solution in the incubation-sample introduction device and the synchronous sample introduction after the incubation are finished are realized; the three-way valve provides a path for conveying liquid; the high-throughput chromatography detection integrated automatic pretreatment device provided by the invention can be used for processing materials such as dairy products, blood, tissues and the like; can be used for sample pretreatment in various fields such as clinical diagnosis, inspection and quarantine, food safety detection, judicial appraisal, drug detection and the like, and has wide market prospect.
Description
Technical Field
The invention relates to a chemical reaction device, in particular to an integrated automatic pretreatment device for high-throughput chromatography detection, and belongs to the technical field of biomedicine.
Background
Chromatography is short for "chromatography", and is exemplified by commonly used immunochromatography, which is based on the principle that a specific antibody is fixed to a specific zone of a nitrocellulose membrane, a sample is dropped onto one end of the membrane, the sample moves forward along the membrane under the action of a capillary, and when the sample moves to the zone where the antibody is fixed, the corresponding antigen in the sample binds specifically to the antibody. In this case, if the analyte is stained with an immunological nanoparticle such as colloidal gold or an immunoenzyme, or a marker molecule having raman, chemiluminescence, up-conversion luminescence, and a time-resolved fluorescence signal is bound thereto, qualitative and quantitative measurement of the analyte can be achieved.
Since chromatographic detection methods are widely applied in a plurality of fields such as medical treatment, food, judicial identification and the like, some chromatographic detection devices are also appeared in the market at present, but the devices have some problems generally. Take the device (test paper strip, detection card) of chemical pollution index in the detection dairy products as an example, its shortcoming lies in: (1) most detection indexes are that the dairy product is directly detected without pretreatment of the milk sample, and the method has the advantages of simple and convenient operation and rapid signal response, and has the biggest defect that the complex matrix environment of the milk sample obviously restricts the detection sensitivity; (2) the inconsistency of the index pretreatment method is detected, so that the high-throughput measurement of the dairy product to be detected is difficult to realize. Most of the current high-throughput devices in the market are high-throughput signal readers; (3) for the detection indexes needing pre-incubation, multichannel synchronous incubation and determination before detection are not realized, and the detection flux is obviously restricted.
Disclosure of Invention
Aiming at the defects in the milk product colloidal gold detection practice, the invention discloses an integrated automatic pretreatment device for high-flux chromatography detection, aiming at integrating the operations of chemical reaction, uniform mixing, centrifugation, incubation, sample introduction and the like involved in the milk product pretreatment into an automatic device, optimizing the sample pretreatment time, improving the pretreatment efficiency of the milk product to be detected, improving the sensitivity and the detection flux of the existing colloidal gold immunoassay product for determining chemical pollutants in the milk product, and realizing the one-stop and automatic pretreatment. In order to achieve the purpose, the invention adopts the following technical scheme:
the high-throughput chromatography detection integrated automatic pretreatment device is an automatic device which integrates operations of chemical reaction, uniform mixing, centrifugation, incubation, sample introduction and the like;
the high-flux chromatographic detection integrated automatic pretreatment device comprises an integrated centrifuge, a plunger pump, a three-way valve, a multi-channel incubation-sample introduction device and three pretreatment methods matched with a dairy product. The integrated centrifuge is a device capable of integrally realizing three pretreatment methods of dairy products; the plunger pump provides power for the whole pretreatment device, so that the milk sample target solution treated in the integrated centrifuge is drawn, and the incubation solution in the incubation-sample introduction device is filled and is synchronously introduced after the incubation is finished; the three-way valve provides a path for conveying liquid; the multichannel incubation-sample introduction device provides a platform for high-throughput chromatography detection pre-incubation and synchronous sample introduction; the three pretreatment methods of the dairy product are an acid-base method, a heavy metal method and an organic solvent method which are screened according to different detection index characteristics;
further, the integrated centrifuge integrates the steps for realizing the pretreatment of the dairy product, which are disclosed in the corresponding patent (an integrated centrifuge, 201711071241.5); the plunger pump forms alternate positive pressure and negative pressure in the pipeline through push-pull action, so that the suction and the delivery of the target solution and the synchronous sample introduction of the incubation solution are realized; the three-way valve may be changed to a four-way valve, a six-way valve, or the like according to the purpose of operation; the steps of synchronous incubation and sample injection of the multi-channel incubation-sample injection device are disclosed in the patent (an incubation sample injection device based on multi-channel immunochromatography detection, 201711071790.2); the three pretreatment methods for dairy products refer to the steps of dairy product treatment in detail in the patent (three rapid pretreatment methods for raw milk, 2017108950985).
The material of the high-flux chromatography detection integrated automatic pretreatment device can be plastic, and can also be all plastic materials such as metal, paper, glass and the like.
The high-throughput chromatography detection integrated automatic pretreatment device provided by the invention can be used for processing materials such as dairy products, blood, tissues and the like; the method can be used for sample pretreatment in various fields such as clinical diagnosis, inspection and quarantine, food safety detection, judicial appraisal, drug detection and the like, can be used in combination with various detection sensors, can also realize big data analysis through data remote transmission, and has wide market prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings obtained from the drawings belong to the protection scope of the present invention for those skilled in the art without creative efforts.
FIG. 1 is a structural diagram of an integrated automatic pretreatment device for high-throughput chromatography detection according to the present invention
In the drawings, the materials represented by the various reference numerals are listed below:
1. an integrated centrifuge; 2. a plunger pump; 3. a three-way valve; 4. a multi-channel incubation-sample introduction device.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment 1, the high-throughput chromatography detection integrated automatic pretreatment device is used for pretreatment of dairy product colloidal gold detection.
The method comprises the following operation steps: firstly, an integrated centrifuge pretreats a milk sample; secondly, negative pressure is provided by the plunger pump, and the processed milk sample is sucked into the plunger pump through the pipeline A; thirdly, positive pressure is provided by a plunger pump, the processed milk sample reaches a three-way valve through a pipeline B, is dripped into an enzyme labeling hole of the integrated chromatography device through a pipeline C, and stands for 3 min; fourthly, alternating positive pressure and negative pressure are provided by a plunger pump, and the enzyme-labeled hole incubation solution is uniformly mixed through a pipeline B, D; providing negative pressure through a plunger pump, and sucking the enzyme-labeled hole incubation solution into a detection column base of the integrated chromatography detection device through a pipeline B, D; based on the action of capillary force, the incubation solution sequentially passes through a sample pad of the chromatography test paper, a gold pad and a detection area quality control area on a nitrocellulose membrane, and finally reaches a water absorption paper pad. Sixthly, after the chromatographic reaction is carried out for 5min, the determination result of the colloidal gold is judged and determined;
the above operation steps are all automatically carried out under the control of a program.
The high-throughput chromatography detection integrated automatic pretreatment device provided by the embodiment of the invention is described in detail above, can effectively reduce the influence of the action of the matrix in the dairy product on the chromatography detection, improves the detection sensitivity and the flux, and is suitable for being matched and used on site. The high-throughput chromatography detection integrated automatic pretreatment device provided by the invention can be used together with various sensing detection devices to collect measurement signals, and can be used for rapid pretreatment of samples in the fields of clinical diagnosis, inspection and quarantine, food safety detection, judicial identification, drug and narcotic detection and the like.
The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (2)
1. The utility model provides an integrate automatic preceding processing apparatus for high flux chromatography detects which characterized in that: the device comprises an integrated centrifuge, a plunger pump, a three-way valve and a multi-channel incubation-sample introduction device, and is matched with three pretreatment methods of dairy products, wherein the integrated centrifuge is an integrated device for realizing the three pretreatment methods of the dairy products, and the operation steps are as follows: adding a rotor into the dairy product and adding a reaction reagent A; placing a capsule reagent B into the rotor clamping groove; starting a motor power supply; fourthly, after the rotor is covered with the composite stirrer, the reagent A and the dairy product to be detected are uniformly mixed by the composite stirrer at a low speed, and a first-step chemical reaction is carried out to generate protein precipitation; fifthly, the operation is switched to high speed, and the denatured protein is rapidly gathered and separated at the bottom of the barrier under the assistance of the barrier; sixthly, converting to medium speed, dissolving and releasing the capsule reagent B in the clamping groove, and performing a second step of chemical reaction; seventhly, repeating the step 5, and separating the newly generated protein precipitate; stopping the centrifugal machine, and collecting the target solution converged by the gravity action at the platform to perform measurement; the plunger pump provides power for the whole pretreatment device, realizes the drawing of target solution after the milk sample is treated in the integrated centrifuge, and synchronously samples after the filling of incubation solution in the incubation-sample introduction device and the incubation are finished, and the operation steps are as follows: placing an enzyme-labeled hole corresponding to an index to be detected in an incubation disc, placing corresponding immunochromatographic test paper in a sealed detection column, and covering the top of the column; adding 200 mul of solution to be detected into each enzyme-labeled hole, and incubating for 2 min; connecting an injector through an opening at the top of the top cover, realizing pressure change between the detection column and the enzyme labeling hole by repeatedly pumping, further realizing full mixing of each incubation solution, pumping again, simultaneously sucking the incubation solution in each enzyme labeling hole into a sample containing area corresponding to each detection column base through negative pressure, and starting the immunochromatography reaction on the chromatography test paper based on capillary action; fourthly, after waiting for 5min, judging and reading the measurement result; the three-way valve provides a path for conveying liquid; the multichannel incubation-sample introduction device provides a platform for high-throughput chromatography detection pre-incubation and synchronous sample introduction; the three pretreatment methods of the dairy product are an acid-base method, a heavy metal method and an organic solvent method which are screened according to different detection index characteristics; the integrated centrifuge is integrated to realize pretreatment of dairy products; the plunger pump forms alternate positive pressure and negative pressure in the pipeline through push-pull action, so that the suction and the conveying of the target solution and the synchronous sample introduction of the incubation solution are realized; one port of the three-way valve is communicated with an outlet of the plunger pump through a pipeline B, the other port of the three-way valve is communicated with a top suction port of the multi-channel incubation-sample introduction device through a pipeline D, the other port of the three-way valve is connected with a pipeline C, and the end part of the pipeline C is aligned with an enzyme labeling hole of the multi-channel incubation-sample introduction device.
2. An integrated automatic pretreatment device for high-throughput chromatography detection according to claim 1, wherein: used for processing dairy products, blood and tissue materials; or used for clinical diagnosis, inspection and quarantine, food safety detection, judicial identification and pretreatment of drug detection samples.
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