CN203705459U - Full-automatic luminescence immunoassay system based on micro-nano magnetic bead electromagnetic transfer technology - Google Patents
Full-automatic luminescence immunoassay system based on micro-nano magnetic bead electromagnetic transfer technology Download PDFInfo
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- CN203705459U CN203705459U CN201320792036.9U CN201320792036U CN203705459U CN 203705459 U CN203705459 U CN 203705459U CN 201320792036 U CN201320792036 U CN 201320792036U CN 203705459 U CN203705459 U CN 203705459U
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Abstract
The utility model provides a full-automatic luminescence immunoassay system based on a micro-nano magnetic bead electromagnetic transfer technology. The full-automatic luminescence immunoassay system comprises a computer and a detection box, wherein the detection box is a sealable light tight box body; the detection box comprises a first motor, a second motor, a membrane rupturing device, an electromagnetic stirring rod, a teflon coating or a one-off plastic sleeve, immunomagnetic beads, a sample tank, a temperature control device, a photoelectric conversion device, a bottom reading type light path, a waste material recycling area or an ultrasonic cleaning device, a reagent storehouse consumable or consumable adapter, a guide rail and an electromagnet. By adopting the full-automatic luminescence immunoassay system based on a magnetic bead transfer method, the detection steps are simplified, the detection module is integrated, a complicated liquid path system is avoided, the complete automation and miniaturization of the immune detection system are achieved while the detection time is saved, and meanwhile, the system cost is reduced.
Description
Technical field
The utility model belongs to medical Instrument field, particularly the required small size automatic luminescence immunoassay system of mobile hospital's laboratory medicine.
Background technology
Automatic immunity system can be at large, improves significantly the specificity of immunization experiment and the sensitivity of reagent, reduced the error that human factor causes.Automatic enzyme immune equipment has been widely used in clinical, is detecting on the basis of principle based on ELISA, as follows according to the different classifications of the solid phase carrier of antibody combination:
(1) container class solid phase carrier, in this type, can be subdivided into again ELISA Plate, plastic tube, plastics rifle point etc., antibody is incorporated on this class solid phase carrier, instrument is realized the processes such as liquid, incubation, cleaning of moving automatically, due to the sterically hindered reason of antigen-antibody solid phase combination, this class methods sensitivity is not high; (2) non-container class solid phase carrier, in this type, can be subdivided into again polystyrene microsphere and magnetic-particle (magnetic bead) etc., certainly such solid phase carrier completes the immune association reaction under accurate even phase condition in need to be at certain reaction vessel, as ELISA Plate, plastic tube, and the full-automatic checkout equipment coordinating with it has also needed to move the process of liquid, incubation and cleaning.These class methods are due to the reaction principle of the even phase of standard adopting, sensitivity has had raising by a relatively large margin compared with the former, particularly apply the mode of magnetic bead, due to the reason of magnetic enrichment, make immunoreactive sensitivity higher, had at present more and more clinical systems to adopt this principle to carry out robotization detection.
But current equipment all need to carry out move sample (liquid), cleaning process, the existence of liquid-way system has not only increased the volume of system cost, system, and extended whole detection time, the possibility that detects cross pollution between sample increases greatly, more and more be not suitable for the medical room demand of hospital's growing tension, obviously can not meet mobile hospital or detect fast needs.
The full-automatic nucleic acid extraction equipment shifting based on magnetic bead has been widely used in nucleic acid extraction, provide reference to us, be different from the nucleic acid extraction equipment that moves liquid formula, due to the nonspecific absorption nucleic acid of magnetic bead, utilize electromagnetism or Permanentmagnet bar to shift magnetic bead in different solution systems, reached the effect of separation and purification nucleic acid.At present existing utilize commercially available nucleic acid extraction equipment complete magnetic enzyme immune reaction, the last manual report of realizing luminous detection in microplate reader, but do not find to utilize magnetic bead transfer method to realize the system that full-automatic immunoluminescence is analyzed.
Utility model content
The purpose of this utility model is exactly the deficiency that will overcome commercially available full-automatic immunoluminescence system, utilize magnetic bead transfer method for basis, simplify detecting step, integrate again detection module, in realizing the full-automation of immunoluminescence detection system, miniaturization, save detection time, avoid cross pollution between sample, reduced system cost simultaneously.
The purpose of this utility model is achieved in that a kind of full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques, comprises computer and detection case; Described detection case is sealable lucifuge casing, is provided with sample cell, attemperating unit, photoelectric conversion device, the end and reads formula light path, useless consumptive material recycle district or ultrasonic cleaning equipment, agent bin consumptive material and consumptive material adapter, guide rail and electromagnet in detection case; Agent bin consumptive material is placed in consumptive material adapter, its middle guide is positioned at the top of sample cell, consumptive material adapter and useless consumptive material recycle district or ultrasonic cleaning equipment, on guide rail, be also provided with film breaking unit and magnetic stirring bar, it is moving that film breaking unit is subject to the first driven by motor can complete 3 maintenance and operations, and magnetic stirring bar is connected with electromagnet and is subject to the secondth driven by motor can complete 3 maintenance and operations and moves and rotate; Magnetic stirring bar outer wall has teflon coatings or disposable plastic cover, the end to read formula light path to be located at consumptive material adapter below, carry out data transmission computer for controlling the duty of the first motor, the second motor and electromagnet by photoelectric conversion device and computer, for receiving data from photoelectric conversion device and analyzing, export data.
Comprise that first, second motor, film breaking unit, electromagnet, magnetic stirring bar, teflon coatings or disposable plastic cover, magnetic bead, sample cell, attemperating unit, photoelectric conversion device, the end read formula light path, useless consumptive material recycle district or ultrasonic cleaning equipment, agent bin consumptive material and consumptive material adapter, guide rail, electromagnet or electromagnetic relay; What said apparatus was all arranged in lucifuge can airtight casing.
Magnetic stirring bar can produce galvanomagnetic effect jointly with electromagnet, obtains under magnetic, off-position and lose magnetism under "on" position; Or magnetic stirring bar this as ferrous metal rod that can magnetic conduction, the galvanomagnetic effect that its magnetic is produced by electromagnet obtains.
On can airtight casing, there is corresponding data interface and be connected with computer, carry out trace routine setting and light signal strength analysis for human-computer interaction.
Motor can complete 3 maintenance and operations and moves and rotate, and comprises along guide rail, vertical guide rail and does movement up and down or around.Drive magnetic stirring bar to complete the function that 3 maintenance and operations are moving and stir.
It is moving that film breaking unit can complete 3 maintenance and operations, before test, the encapsulated membranes on agent bin consumptive material crushed, and drives teflon coatings or disposable plastic to cover into convenient electromagnetic stirring rod.
On magnetic stirring bar, scribble teflon coatings or before detection is carried out, be inserted in disposable plastic cover, be convenient to detect the rear cleaning to magnetic stirring bar, prevent the cross pollution of sample room.Teflon coatings or disposable plastic cover are paddle form, are beneficial to and mix reactant liquor.If disposable plastic nested structure, also should have the design of moving back consumptive material parts on magnetic stirring bar.
Magnetic stirring bar can conduct electricity magnet magnetic, and then absorption or discharge immunomagnetic beads.Reactant liquor in immunomagnetic beads or agent bin consumptive material intercepts via teflon coatings or disposable plastic cover, can directly not contact with magnetic stirring bar.
Attemperating unit is that room temperature～40 are ℃ adjustable, and its accuracy of temperature control is ± 0.5 ℃.In order to immunoreactive optimum temperature to be provided.
Formula light path is read for collecting chemiluminescence signal after immune response in the end, is converted to electric signal by photoelectric conversion device, analyzes output signal by data-interface on computer.
Useless consumptive material recycle district or ultrasonic cleaning equipment will adopt selective design, in the time adopting teflon coatings on magnetic stirring bar, will be designed to ultrasonic cleaning equipment, be adsorbed in magnetic bead or the residual sample on teflon coatings for cleaning physics.In the time adopting disposable plastic Analysis of Nested Design on magnetic stirring bar, will be designed to useless consumptive material recycle district, this disposable plastic cover discarding after finishing dealing with for recovery sample.
Agent bin consumptive material and adapter are packaged type.While detection, fix and be placed on attemperating unit, photoelectric conversion device and the end and read in formula light path by manually required consumptive material being put into consumptive material adapter, in consumptive material, be sealed with respectively immunomagnetic beads, cleansing solution, labelled antibody and chemical illuminating reagent.
Before test, magnetic stirring bar is subject to the second driven by motor to hover over the top of useless consumptive material recycle district or ultrasonic cleaning equipment, for film breaking unit provides movably space, after film breaking unit is crushed the encapsulated membranes on agent bin consumptive material by the first driven by motor, reset rests on the Far Left of guide rail, for magnetic stirring bar provides movably space.While detection, magnetic stirring bar obtains magnetic, first absorption immunomagnetic beads is placed in sample cell, after electromagnet power-off, magnetic stirring bar magnetic disappears, immunomagnetic beads is released in sample cell, utilize the Stirring function of magnetic stirring bar to mix immunomagnetic beads, under uniform temperature condition, make the thing to be detected in immunomagnetic ca pture sample.After electromagnet energising, magnetic stirring bar obtains magnetic, and (magnetic separation) after the immunomagnetic beads compound of thing to be detected caught in absorption, is transferred in cleaning fluid agent bin, discharges after immunomagnetic beads compound agitator treating after demagnetization.After magnetic separates, absorption immunomagnetic beads compound proceeds in labelled antibody agent bin, stirs and evenly mixs, and under uniform temperature condition, labelled antibody is combined with immunomagnetic beads compound.After separating, magnetic is transferred to next washing storehouse in connection with the immunomagnetic beads compound that has labelled antibody, repeat the step of stirring and washing, magnetic is transferred to chemical illuminating reagent storehouse by this compound after separating again, utilizes photoelectric conversion device to collect light signal, and then analyzes determinand information.
The beneficial effects of the utility model are: without liquid-way system, system bulk and cost significantly reduce and reduce.Save simultaneously and inhale tapping step, shortened detection time.
Full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques of the present utility model compared with prior art has following features:
One, the utility model product provides portable small product size, can be used for mobile hospital;
Two, the utility model product provides and has stirred with energy, can better make reactive component see abundant contact.Increase sensitivity.
Three, the utility model product provides the full-automatic integrated solution take magnetic bead transfer method as basis, has reduced infection and the systematic error of human intervention.
Accompanying drawing explanation
Fig. 1 is the structural representation of the full-automatic illumination immunoassay system of the utility model based on micro-nano magnetic bead electromagnetism transfer techniques.
1-the first motor, 2-the second motor, 3-film breaking unit, 4-magnetic stirring bar, 5-teflon coatings or disposable plastic cover, 6-immunomagnetic beads, 7-sample cell, 8-attemperating unit, 9-photoelectric conversion device, at the bottom of 10-, read formula light path, 11-consumptive material adapter, the useless consumptive material recycle district of 12-or ultrasonic cleaning equipment, 13-agent bin consumptive material, 14-guide rail, 15-electromagnet.
Embodiment
Below by the mode of embodiment, technical solutions of the utility model are elaborated, but protection domain of the present utility model is not limited to described embodiment.
Embodiment 1
1, self check and the preparatory stage
As shown in Figure 1, start preheating self check, sets attemperating unit 8 temperature stabilizations at 37 ℃, sets hemodilution sample to be measured is put into sample cell 7, and corresponding agent bin consumptive material 13 is put into after consumptive material adapter 11, is placed in system fixed position.Airtight casing, first film breaking unit 3 crushes the encapsulated membranes on kit consumptive material 13 along guide rail 14 under the first motor 1 drives.
2, the immunomagnetic ca pture sample stage
Magnetic stirring bar 4 moves to above the container that stores immunomagnetic beads 6 in kit consumptive material 13 along guide rail 14 under the drive of the second motor 2, promote down to go deep into this agent bin by the second motor 2 again, stir and evenly mix immunomagnetic beads 6 reagent, after electromagnet 15 energisings, magnetic stirring bar 4 obtains magnetic, and absorption immunomagnetic beads 6 is outside teflon coatings or disposable plastic cover 5.Magnetic stirring bar 4 moves to above sample cell 7 along guide rail 13 under the drive of the second motor 2, promote down to go deep into this agent bin by the second motor 2 again, after electromagnet 15 power-off, magnetic stirring bar 4 loses magnetism, the immunomagnetic beads 6 being adsorbed in outside teflon coatings or disposable plastic cover 5 is released into sample cell 7, magnetic stirring bar 4 stirs and evenly mixs 6,37 ℃ of sample and immunomagnetic beadses and stirs and evenly mixs 5min.After electromagnet 15 energisings, magnetic stirring bar 4 obtains magnetic, and absorption immunomagnetic beads 6 capture antigens are outside teflon coatings or disposable plastic cover 5.Promoted down by the second motor 2, mention magnetic stirring bar 4.
3, the antigen of non-specific binding is removed in washing
Magnetic stirring bar 4 moves to above cleansing solution a agent bin along guide rail 13 under the drive of the second motor 2, promote down to go deep into this agent bin by the second motor 2 again, after electromagnet 15 power-off, magnetic stirring bar 4 loses magnetism, the antigenic compound that is adsorbed in the immunomagnetic beads 6 outside teflon coatings or disposable plastic cover 5 and catch is released into the agent bin of cleansing solution a, magnetic stirring bar 4 stirs and evenly mixs compound, and 37 ℃ stir and evenly mix 5min.After electromagnet 15 energisings, magnetic stirring bar 4 obtains magnetic, and complete immunomagnetic beads 6 capture antigens of absorption washing overlap outside 5 in teflon coatings or disposable plastic.Promoted down by the second motor 2, mention magnetic stirring bar 4.
4, incorporation of markings antigen
Magnetic stirring bar 4 moves to above labelled antibody agent bin along guide rail 13 under the drive of the second motor 2, promote down to go deep into this agent bin by the second motor 2 again, after electromagnet 15 power-off, magnetic stirring bar 4 loses magnetism, be adsorbed in the immunomagnetic beads 6 outside teflon coatings or disposable plastic cover 5 and the antigenic compound of catching and be released into labelled antibody agent bin, magnetic stirring bar 4 stirs and evenly mixs compound, and 37 ℃ stir and evenly mix 5min.After electromagnet 15 energisings, magnetic stirring bar 4 obtains magnetic, and absorption immunomagnetic beads 6 double antibodies sandwich compounds are outside teflon coatings or disposable plastic cover 5.Promoted down by the second motor 2, mention magnetic stirring bar 4.
5, the labelled antibody of non-specific binding is removed in washing
Magnetic stirring bar 4 moves to above cleansing solution b agent bin along guide rail 13 under the drive of the second motor 2, promote down to go deep into this agent bin by the second motor 2 again, after electromagnet 15 power-off, magnetic stirring bar 4 loses magnetism, the immunomagnetic beads 6 double antibodies sandwich compounds that are adsorbed in outside teflon coatings or disposable plastic cover 5 are released into cleansing solution b agent bin, magnetic stirring bar 4 stirs and evenly mixs compound, and 37 ℃ stir and evenly mix 5min.After electromagnet 15 energisings, magnetic stirring bar 4 obtains magnetic, and absorption combines the immunomagnetic beads 6 capture antigen compounds of labelled antibody outside teflon coatings or disposable plastic cover 5.Promoted down by the second motor 2, mention magnetic stirring bar 4.According to clean result, this step can repeat 1-2 time in new cleansing solution b agent bin, to guarantee specificity.
6, chemiluminescence detection
Magnetic stirring bar 4 moves to above chemical illuminating reagent storehouse along guide rail 13 under the drive of the second motor 2, promote down to go deep into this agent bin by the second motor 2 again, after electromagnet 15 power-off, magnetic stirring bar 4 loses magnetism, the immunomagnetic beads 6 double antibodies sandwich compounds that are adsorbed in outside teflon coatings or disposable plastic cover 5 are released into chemical illuminating reagent storehouse, magnetic stirring bar 4 stirs and evenly mixs compound, is promoted down by the second motor 2, mentions magnetic stirring bar 4.On labelled antibody, material can react with chemical illuminating reagent and produce chemiluminescence, and formula light path 10 is read for collecting the rear chemiluminescence signal of reaction in the end, is converted to electric signal by photoelectric conversion device 9, analyzes output signal by data-interface on computer.
7, abandon useless consumptive material or cleaning step
Magnetic stirring bar 4 moves to above useless consumptive material recycle district or ultrasonic cleaning equipment 12 along guide rail 13 under the drive of the second motor 2, is now divided into two kinds of designs:
If on magnetic stirring bar 4 be teflon coatings 5, promote down to go deep into this agent bin by the second motor 2, in ultrasonic cleaning equipment 12, carry out ultrasonic cleaning, dispose possible non-specific adsorption;
If magnetic stirring bar 4 outer be 5 designs of disposable plastic cover,, directly by taking off consumptive material design part on motor, push disposable plastic and overlap 5 to useless consumptive material recycle district 12.
Magnetic stirring bar 4 resets, and whole system completes testing process.
Claims (5)
1. the full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques, comprises computer and detection case; It is characterized in that described detection case is sealable lucifuge casing, in detection case, be provided with sample cell (7), attemperating unit (8), photoelectric conversion device (9), the end and read formula light path (10), useless consumptive material recycle district or ultrasonic cleaning equipment (12), agent bin consumptive material (13) and consumptive material adapter (11), guide rail (14) and electromagnet (15); Agent bin consumptive material (13) is positioned in consumptive material adapter (11), guide rail (14) is positioned at the top of sample cell (7), consumptive material adapter (11) and useless consumptive material recycle district or ultrasonic cleaning equipment (12), on guide rail (14), be also provided with film breaking unit (3) and magnetic stirring bar (4), film breaking unit (3) is driven by the first motor (1), can complete 3 maintenance and operations moving, magnetic stirring bar (4) is connected with electromagnet (15) and driven by the secondth motor (2), can complete 3 maintenance and operations and moves and rotate; Magnetic stirring bar (4) outer wall has teflon coatings or disposable plastic cover (5), the end to read formula light path (10) to be located at consumptive material adapter (11) below, to carry out data transmission by photoelectric conversion device (9) and computer; Computer is used for controlling the duty of the first motor (1), the second motor (2) and electromagnet (14), and for receiving and analytic signal from photoelectric conversion device (9), to export data.
2. the full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques according to claim 1, teflon coatings or disposable plastic cover (5) are paddle shape.
3. the full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques according to claim 1, immunomagnetic beads (6) is the magnetic material that adopts particle diameter 100nm～5 μ m.
4. the full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques according to claim 1, formula light path (10) is read for collecting chemiluminescence signal after immune response in the end, be converted to electric signal by photoelectric conversion device (9), on computer, analyze output signal by data-interface.
5. the full-automatic illumination immunoassay system based on micro-nano magnetic bead electromagnetism transfer techniques according to claim 1, agent bin consumptive material (13) and consumptive material adapter (11) are packaged type.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320792036.9U CN203705459U (en) | 2013-12-03 | 2013-12-03 | Full-automatic luminescence immunoassay system based on micro-nano magnetic bead electromagnetic transfer technology |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320792036.9U CN203705459U (en) | 2013-12-03 | 2013-12-03 | Full-automatic luminescence immunoassay system based on micro-nano magnetic bead electromagnetic transfer technology |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604939A (en) * | 2013-12-03 | 2014-02-26 | 南京医科大学第二附属医院 | Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique |
| CN104087508A (en) * | 2014-07-18 | 2014-10-08 | 东南大学 | Electromagnetic biological sample processing device |
| CN107475076A (en) * | 2017-09-21 | 2017-12-15 | 中国动物疫病预防控制中心 | Automatic instrument for extracting nucleic acid pig blue-ear disease antibody immune magnetic beads method detection kit |
| CN111304290A (en) * | 2020-03-16 | 2020-06-19 | 通用生物系统（安徽）有限公司 | Nucleic acid extraction method for gene sequencing |
-
2013
- 2013-12-03 CN CN201320792036.9U patent/CN203705459U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604939A (en) * | 2013-12-03 | 2014-02-26 | 南京医科大学第二附属医院 | Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique |
| CN104087508A (en) * | 2014-07-18 | 2014-10-08 | 东南大学 | Electromagnetic biological sample processing device |
| CN107475076A (en) * | 2017-09-21 | 2017-12-15 | 中国动物疫病预防控制中心 | Automatic instrument for extracting nucleic acid pig blue-ear disease antibody immune magnetic beads method detection kit |
| CN111304290A (en) * | 2020-03-16 | 2020-06-19 | 通用生物系统（安徽）有限公司 | Nucleic acid extraction method for gene sequencing |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140709 Effective date of abandoning: 20150923 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20140709 Effective date of abandoning: 20150923 |
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| RGAV | Abandon patent right to avoid regrant |