CN111190004B

CN111190004B - Instant detection system of immunochromatography test strip

Info

Publication number: CN111190004B
Application number: CN202010027561.6A
Authority: CN
Inventors: 吴勇
Original assignee: Shanghai Taihui Biotechnology Co ltd
Current assignee: Shanghai Taihui Biotechnology Co ltd
Priority date: 2020-01-10
Filing date: 2020-01-10
Publication date: 2023-11-10
Anticipated expiration: 2040-01-10
Also published as: CN111190004A

Abstract

The present disclosure relates to an immunochromatographic test strip instant detection system. The detection system comprises an information acquisition device and a calculation device which are separated from each other; the information acquisition device comprises an acquisition component and a first communication component, and the computing device comprises a control module, a storage module, a second communication module and an output module. The detection system simulates and obtains a calibration curve at the actual ambient temperature, thereby remarkably improving the accuracy of the concentration measurement of the luminescent probe.

Description

Instant detection system of immunochromatography test strip

Technical Field

The present disclosure relates to the technical field of in vitro diagnosis of medical apparatuses, and in particular, to an immunochromatographic test strip instant detection system and an instant detection method.

Background

The instant detection means that non-professional detection staff uses a portable instrument to rapidly diagnose and analyze the specimen sample of the patient, and has the characteristics of convenient carrying, simple operation, field inspection and the like. Immunochromatography is one of the most important means for detection in real time. The immunochromatography technology quantitatively detects the markers on the test strip by detecting fluorescence or afterglow generated by excitation on the test strip.

In particular, immunochromatographic techniques immobilize specific antibodies to a certain zone (e.g., T-line and C-line) of nitrocellulose membranes. When one end of the dried nitrocellulose membrane is immersed in or dripped with a sample (urine or serum), the antigen substance to be detected in the sample and the luminescent conjugate thereof with the luminescent probe migrate on the nitrocellulose membrane made of the strip-shaped fibers by means of capillary action. The luminous conjugate of the antigen substance to be detected is combined with the specific antibody on the zone to generate specific immune reaction. Thus, the zone displays a certain color or emits fluorescence or afterglow with a certain intensity under the excitation of excitation light, thereby realizing qualitative or quantitative specific immunodiagnosis.

Currently, detection instruments used in immunochromatography mainly include scanning type detection instruments and imaging type detection instruments. The scanning detector consists of an optical module and a mechanical scanning structure. The excitation diode of the optical module excites the luminescent probe on the test strip, and fluorescence generated by excitation of the luminescent probe is received by the detector such as the photodiode after being converged. The detector can only detect the local fluorescence of the test paper strip at a time, so that a transmission mechanism is required to drive an optical module or a test paper strip tray to carry out scanning detection on the whole test paper strip, a fluorescence curve is obtained after more than ten seconds, and the content of a sample is calculated. The scanning detector is simple to operate, but has low measuring speed, and the measured sample content has larger deviation from the actual value.

The imaging detector uses a fluorescence imaging camera to perform one-time snapshot imaging on the test strip, and then processes the image. The imaging detector has higher requirements on hardware performance such as an operation host, an imaging camera, a light-emitting diode light source and the like, and has the characteristics of large volume, complex specimen identification, complex operation, professional operation and the like. The instrument is used in laboratory, and is fresh in daily clinical test.

It is also well known that ambient temperature is one of the most important parameters of the immune response, and that the immune calibration curve is different for each ambient temperature. Currently, detectors in the industry store only calibration curves at 37 ℃, which requires incubation of the test environment to 37 ℃, which would otherwise lead to inaccurate luminescent probe concentrations. When incubating a strip at an measured ambient temperature other than 37 ℃, it is necessary that the incubation unit incubate for approximately half an hour. The incubation unit additionally increases the size and weight of the detector, the operating steps, and the time of measurement per sample.

Disclosure of Invention

It is an object of the present disclosure to provide an immunochromatographic strip immediate detection system that overcomes at least one of the drawbacks of the prior art.

The subject technology of the present disclosure is illustrated in accordance with the various aspects described below. For convenience, various examples of aspects of the subject technology are described as numbered clauses (1, 2, 3, etc.). These terms are provided as examples and not to limit the subject technology of the present disclosure.

1. An immunochromatographic test strip on-line detection system, wherein:

the immunochromatographic test strip instant detection system comprises an information acquisition device and a calculation device which are separated from each other, wherein the test strip comprises a light-emitting area, and the test strip and/or the information acquisition device comprises a temperature sensing pattern;

The information acquisition device comprises an acquisition component and a first communication component, wherein the acquisition component transmits excitation light to the test strip, acquires light-emitting information and temperature sensing pattern information of a light-emitting area of the test strip, and transmits the acquired light-emitting information and temperature sensing pattern information of the light-emitting area of the test strip to the computing device, and the light-emitting information comprises the light-emitting intensity of the light-emitting area of the test strip;

the computing device comprises a control module, a storage module, a second communication module and an output module, wherein the storage module stores the corresponding relation between the temperature sensing pattern information and the ambient temperature and the luminous intensity of the luminous probe at a plurality of preset temperatures and the luminous probe concentration calibration curve, the second communication module and the first communication module are communicated with each other in a wireless mode or a wired mode,

the control module obtains the luminous information and the temperature sensing pattern information of the luminous area of the test strip through the communication between the first communication component and the second communication module, and the control module invokes the corresponding relation between the temperature sensing pattern information and the ambient temperature from the storage module to obtain the ambient temperature T0 around the test strip, and judges whether the ambient temperature T0 is equal to one of the preset temperatures:

Under the condition that the ambient temperature T0 is equal to one of the preset temperatures, the control module retrieves a luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the ambient temperature T0 from the storage module;

under the condition that the environment temperature T0 is not equal to any one of the preset temperatures, the control module selects a preset temperature T1 and a preset temperature T2 which are adjacent to the environment temperature T0 from top to bottom, and calls a luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1 and a luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2 from the storage module, and the control module simulates a luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the environment temperature T0 through the luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1 and the luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2;

the control module fits the luminous probe concentration value of the luminous area of the test strip according to the luminous intensity of the luminous probe at the ambient temperature T0, the luminous probe concentration calibration curve L0 and the obtained luminous information of the luminous area of the test strip, and outputs the luminous probe concentration value of the luminous area of the test strip through the output module.

2. The immunochromatographic strip immediate detection system according to clause 1, wherein: the control module obtains n luminous probe luminous intensity values corresponding to n luminous probe concentration values at a preset temperature T1 by utilizing a luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1, obtains n luminous probe luminous intensity values corresponding to n luminous probe concentration values at the preset temperature T2 by utilizing a luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2, and obtains the n luminous probe concentration values and the corresponding luminous probe luminous intensity values at an environment temperature T0 by a linear interpolation method from the n luminous probe luminous intensity values and the corresponding n luminous probe luminous intensity values at the preset temperature T1 and the n luminous probe luminous intensity values and the corresponding n luminous probe luminous intensity values at the preset temperature T2, wherein n is an integer greater than 1.

3. The immunochromatographic strip immediate detection system according to clause 2, wherein: the control module simulates a calibration curve L0 of the luminous probe luminous intensity-luminous probe concentration at the ambient temperature T0 by using a logarithmic log-log 4p model or Spline curve Spline function from the n luminous probe concentration values and the corresponding luminous probe luminous intensity values at the ambient temperature T0.

4. The immunochromatographic strip immediate detection system according to clause 1, wherein: the temperature sensing pattern is made of temperature sensing color-changing ink.

5. The immunochromatographic strip immediate detection system according to clause 4, wherein: the thermochromic ink comprises cholesteric liquid crystal temperature-sensitive ink.

6. The immunochromatographic strip immediate detection system according to clause 4, wherein: the temperature sensing pattern comprises a plurality of adjacent small blocks, and each small block changes color at different critical temperatures due to different material proportions of the temperature sensing color-changing ink.

7. The immunochromatographic strip immediate detection system according to clause 1, wherein: the luminescence information comprises the luminescence intensity of fluorescence emitted by the fluorescent probe on the test strip.

8. The immunochromatographic strip immediate detection system according to clause 1, wherein: the luminous information comprises the luminous intensity of afterglow emitted by a long afterglow luminous probe on the test paper.

9. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the card shell of the test strip is provided with an identification code, and the identification code contains traceability data bound with the specimen sample.

10. The immunochromatographic strip immediate detection system according to clause 9, wherein: the acquisition component is configured to also acquire identification code information of the test strip, and the control module identifies traceability data of the test strip from the identification code information.

11. The immunochromatographic strip immediate detection system according to clause 9, wherein: the identification code is a two-dimensional code or a bar code.

12. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the first communication assembly and the second communication module are wireless communication devices or wired communication devices used in cooperation.

13. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the acquisition component comprises a light source, a filter and a camera, wherein the light source is configured to send excitation light to the test strip, and the camera is configured to penetrate the filter to take color pictures of the test strip.

14. The fluorescence immunochromatographic strip on-line detection system according to clause 13, wherein: the test strip is placed opposite the camera and the filter, and the light source is disposed offset from the paths of the camera and the filter, and the test strip opposite each other.

15. The immunochromatographic strip immediate detection system according to clause 13, wherein: the light source is an LED lamp with a diaphragm.

16. The immunochromatographic strip immediate detection system according to clause 13, wherein: the camera is a wide-angle digital camera.

17. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the information acquisition device comprises an upper shell and a lower shell, and the upper shell and the lower shell are matched together to form a light-proof cavity inside.

18. The immunochromatographic strip immediate detection system according to clause 17, wherein: a supporting frame is arranged in the light-shading cavity and fixed on the lower shell.

19. The immunochromatographic strip immediate detection system according to clause 18, wherein: the support frame includes a plurality of compartments configured to support a plurality of parts of the information gathering device.

20. The fluorescence immunochromatographic strip on-line detection system according to clause 17, wherein: the information collection device includes a test strip receiving slot that is fixed to the upper housing or the lower housing and that opens at the respective housing to receive an inserted test strip.

21. The fluorescence immunochromatographic strip on-line detection system according to any one of clauses 1 to 8, wherein: the information acquisition device comprises a power supply assembly, and the power supply assembly supplies power to the information acquisition device.

22. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the length of the information acquisition device is not more than 10cm, the width is not more than 8cm, and the height is not more than 10cm.

23. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the computing device is a mobile terminal installed with a detection program, or a part of the computing device is provided in the mobile terminal installed with the detection program, and another part is provided in a server in communication with the mobile terminal.

24. The immunochromatographic strip immediate detection system according to any one of clauses 1 to 8, wherein: the test strip comprises a cartridge and a nitrocellulose membrane arranged in the cartridge, wherein the cartridge is provided with a sample loading port and a display port which are axially spaced, and the display port is the light-emitting area.

25. A method for detecting a test strip in real time by using an immunochromatographic test strip in real time detection system, wherein the method comprises the following steps:

inserting a test strip into the information acquisition device, and starting a detection command in the computing device;

the information acquisition device transmits excitation light to the test strip, and acquires light-emitting information and temperature sensing pattern information of a light-emitting area of the test strip, wherein the light-emitting information comprises the light-emitting intensity of the light-emitting area of the test strip;

the computing device is communicated with the computing device in a wireless mode or a wired mode to obtain the luminous information and the temperature sensing pattern information of the luminous area of the test strip;

The computing device obtains the ambient temperature T0 around the test strip based on the corresponding relation between the preset temperature sensing pattern information and the ambient temperature, and judges whether the ambient temperature T0 is equal to one of the preset temperatures:

under the condition that the ambient temperature T0 is equal to one of the preset temperatures, the computing device invokes a luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the ambient temperature T0;

when the ambient temperature T0 is not equal to any one of the preset temperatures, the computing device selects a preset temperature T1 and a preset temperature T2 which are adjacent to the ambient temperature T0 up and down, and invokes a luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1 and a luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2, and the computing device simulates a luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the ambient temperature T0 through the luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1 and the luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2;

the calculating device fits the luminous probe concentration value of the luminous area of the test strip according to the luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the ambient temperature T0 and the obtained luminous information of the luminous area of the test strip, and outputs the luminous probe concentration value of the luminous area of the test strip.

26. The detection method of clause 25, wherein: the information acquisition device acquires identification code information of the test strip during the period of sending the excitation light, and the computing device identifies tracing data of the test strip from the identification code information and outputs the tracing data of the test strip.

27. The detection method of clause 25 or 26, wherein: the immunochromatographic test strip on-line detection system is according to any one of clauses 1 to 24.

28. An immunochromatographic test strip instant detection method, wherein the method comprises the following steps:

receiving luminous information and temperature sensing pattern information of a luminous area of the test strip;

based on the corresponding relation between the preset temperature sensing pattern information and the ambient temperature, obtaining the ambient temperature T0 around the test strip, and judging whether the ambient temperature T0 is equal to one of a plurality of preset temperatures:

under the condition that the ambient temperature T0 is equal to one of the preset temperatures, a luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the preset ambient temperature T0 is called;

under the condition that the ambient temperature T0 is not equal to any one of the preset temperatures, selecting a preset temperature T1 and a preset temperature T2 which are adjacent to the ambient temperature T0 from top to bottom, and calling a luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1 and a luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2, and simulating a luminous probe luminous intensity-luminous probe concentration calibration curve L0 at the ambient temperature T0 through the luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1 and the luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2;

Fitting to obtain a luminescent probe concentration value of the luminescent region of the test strip according to the luminescent probe luminescent intensity-luminescent probe concentration calibration curve L0 at the ambient temperature T0 and the obtained luminescent information of the luminescent region of the test strip, and outputting the luminescent probe concentration value of the luminescent region of the test strip.

29. The detection method of clause 28, wherein: obtaining n luminous probe luminous intensity values corresponding to n luminous probe concentration values at a preset temperature T1 by utilizing a luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1, obtaining n luminous probe luminous intensity values corresponding to n luminous probe concentration values at the preset temperature T2 by utilizing a luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2, and obtaining the n luminous probe concentration values and the corresponding luminous probe luminous intensity values at an environment temperature T0 by utilizing a linear interpolation method from the n luminous probe luminous intensity values and the corresponding luminous probe luminous intensity values at the preset temperature T1 and the n luminous probe luminous intensity values and the corresponding luminous probe luminous intensity values at the preset temperature T2, wherein n is an integer greater than 1.

30. The detection method of clause 28, wherein: from the n luminescent probe concentration values and the corresponding n luminescent probe luminescence intensity values at ambient temperature T0, a luminescent probe luminescence intensity-luminescent probe concentration calibration curve L0 at ambient temperature T0 is simulated by using a logarithmic log-log 4p model or Spline curve Spline function.

31. The detection method of any one of clauses 28-30, wherein: and receiving the identification code information of the test strip, identifying the tracing data of the test strip from the identification code information, and outputting the tracing data of the test strip.

32. A computing device, wherein the computing device comprises:

one or more processors; and

one or more memory modules configured to store a series of computer-executable instructions and computer-accessible data associated with the series of computer-executable instructions,

wherein the series of computer-executable instructions, when executed by the one or more processors, cause the one or more processors to perform the method of any of clauses 28-31.

33. A non-transitory computer-readable storage medium having stored thereon a series of computer-executable instructions that, when executed by one or more computing devices, cause the one or more computing devices to perform the method of any of clauses 28-31.

34. An information acquisition device for immunochromatography test strip real-time detection, wherein the information acquisition device comprises:

an upper housing and a lower housing fitted together to form a light-shielding cavity inside;

a test strip receiving slot positioned within the light-protected cavity and configured to open on the upper or lower housing to receive an inserted test strip, the test strip including a light emitting region having a long afterglow light emitting probe; and

the collecting component is positioned in the photophobic cavity and comprises a light source for transmitting excitation light to the inserted test strip, a filter and a camera for shooting the test strip, the filter comprises a double-filter switcher consisting of an infrared cut-off filter and full-spectrum optical glass, the double-filter switcher can switch between the infrared cut-off filter and the full-spectrum optical glass,

the double-filter switcher is configured to switch the filter to the infrared cut-off filter in the excitation period of the long-afterglow luminous probe so as to filter out high-intensity excitation light, and the camera can shoot a color picture on the test strip through the infrared cut-off filter; and the filter is switched to the full-spectrum optical glass in the afterglow period of the long afterglow luminous probe, so that the camera can shoot a color picture on the test strip through the full-spectrum optical glass.

35. The information gathering device of clause 34, wherein the test strip receiving slot is disposed opposite the camera and the filter, and the light source is disposed offset from a path between the camera and the filter, and the test strip receiving slot opposite each other.

36. The information gathering device as recited in clause 34 wherein the light source is an LED lamp with a diaphragm.

37. The information gathering device as recited in clause 34 wherein the camera is a wide angle digital camera.

38. The information gathering device as recited in clause 34, wherein the combination of the filter and the camera is an IR-Cut camera.

39. The information gathering device as recited in any of clauses 34-38, wherein the information gathering device further comprises a communication component positioned within the light-protected cavity, the communication component configured to transmit the captured color photograph to a computing device separate from the information gathering device.

40. The information gathering device as recited in clause 39, wherein the communication component is a wireless communication component or a wired communication component.

41. The information gathering device as recited in any of clauses 34-38, wherein the information gathering device further comprises a power supply assembly for supplying power thereto.

42. The information gathering device as recited in any of clauses 34-38, wherein the information gathering device further comprises a support bracket positioned within the light-shielding cavity and secured to the lower housing.

43. The information gathering device as recited in clause 42, wherein the support frame comprises a plurality of compartments configured to support the gathering assembly.

44. The information collecting apparatus according to any one of clauses 34-38, wherein the information collecting apparatus further comprises a temperature sensing pattern provided on the lower case or a support frame of the lower case and capable of being photographed by the camera.

45. The information gathering device of clause 44, wherein: the temperature sensing pattern is made of temperature sensing color-changing ink.

46. The information gathering device of clause 45, wherein: the thermochromic ink comprises cholesteric liquid crystal temperature-sensitive ink.

47. The information gathering device of clause 44, wherein: the temperature sensing pattern is arranged into a plurality of adjacent small blocks, and each small block changes color at different critical temperatures due to different material proportions of the temperature sensing color-changing ink.

48. The information gathering device as recited in any of clauses 34-38, wherein the information gathering device has a length of no greater than 10cm, a width of no greater than 8cm, and a height of no greater than 10cm.

49. The test strip for testing the specimen sample comprises a card shell, wherein a temperature sensing pattern capable of collecting the ambient temperature around the test strip is arranged on the card shell.

50. The test strip of clause 49, wherein the temperature sensitive pattern is made of a thermochromic ink.

51. The test strip of clause 50, wherein: the thermochromic ink comprises cholesteric liquid crystal temperature-sensitive ink.

52. The test strip of clause 50, wherein: the temperature sensing pattern comprises a plurality of small blocks, and each small block changes color at different critical temperatures due to different material proportions of the temperature sensing color-changing ink.

53. The test strip of clause 52, wherein: the plurality of small blocks are arranged according to the order of the critical temperature.

54. The test strip of clause 53, wherein: the temperature sensing pattern is rectangular, and the plurality of small blocks are arranged in a manner of a plurality of rows and a plurality of columns.

55. The test strip of clause 53, wherein: the temperature sensing pattern is in a straight shape, and the plurality of small blocks are arranged in a row manner.

56. The test strip of clause 53, wherein: the temperature sensing pattern is ring-shaped, and the plurality of small blocks are arranged in a ring shape.

57. The test strip of clause 49, wherein: the temperature sensing pattern is arranged on the card shell through printing or pasting.

58. The test strip of any one of clauses 49-57, wherein: the card shell is also provided with an identification code adjacent to the temperature sensing pattern, and the identification code contains traceable data bound with the specimen sample.

59. The test strip of clause 58, wherein: the identification code is a two-dimensional code or a bar code.

60. The test strip of clause 58, wherein: the identification code is arranged on the card shell by printing or pasting.

61. The test strip of any one of clauses 49-57, wherein: the test strip also includes a nitrocellulose membrane within the cartridge, and the test strip has an axially spaced loading port and a display port.

62. The test strip of clause 61, wherein: the temperature sensing pattern is arranged on one side of the card shell, which is far away from the sample loading port, of the display port.

63. The test strip of clause 61, wherein: the display port includes a nitrocellulose membrane exposed in the display port area and T and C lines for testing.

Additional features and advantages of the subject technology of the present disclosure will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the subject technology of the present disclosure. The advantages of the subject technology of the present disclosure will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the subject technology of the present disclosure as claimed.

Drawings

The various aspects of the disclosure will be better understood upon reading the following detailed description in conjunction with the drawings in which:

FIG. 1 shows a schematic diagram of an immunochromatographic test strip point-of-care detection system according to an embodiment of the present disclosure;

FIG. 2 shows a front view of a test strip to be detected by the immunochromatographic test strip on-line detection system of FIG. 1;

FIGS. 3 and 4 show exploded and assembled perspective views of the immunochromatographic strip instant detection system information-collecting device of FIG. 1;

FIG. 5 shows a graph of luminescence characteristics of a long afterglow luminescent probe;

FIG. 6 shows a perspective view of the IR-Cut camera at different angles; and

FIG. 7 shows a flow chart of a detection method of the immunochromatographic strip immediate detection system of FIG. 1.

Detailed Description

The present disclosure will be described below with reference to the accompanying drawings, which illustrate several embodiments of the present disclosure. It should be understood, however, that the present disclosure may be presented in many different ways and is not limited to the embodiments described below; indeed, the embodiments described below are intended to more fully convey the disclosure to those skilled in the art and to fully convey the scope of the disclosure. It should also be understood that the embodiments disclosed herein can be combined in various ways to provide yet additional embodiments.

It should be understood that throughout the drawings, like reference numerals refer to like elements. In the drawings, the size of certain features may be modified for clarity.

It should be understood that the terminology used in the description is for the purpose of describing particular embodiments only, and is not intended to be limiting of the disclosure. All terms (including technical and scientific terms) used in the specification have the meanings commonly understood by one of ordinary skill in the art unless otherwise defined. Well-known functions or constructions may not be described in detail for brevity and/or clarity.

As used in this specification, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. The use of the terms "comprising," "including," and "containing" in the specification mean that the recited features are present, but that one or more other features are not excluded. The use of the phrase "and/or" in the specification includes any and all combinations of one or more of the associated listed items. The words "between X and Y" and "between about X and Y" used in this specification should be interpreted to include X and Y. The phrase "between about X and Y" as used herein means "between about X and about Y", and the phrase "from about X to Y" as used herein means "from about X to about Y".

In the description, an element is referred to as being "on," "attached" to, "connected" to, "coupled" to, "contacting" or the like another element, and the element may be directly on, attached to, connected to, coupled to or contacting the other element or intervening elements may be present. In contrast, when an element is referred to as being "directly on," "directly attached to," directly connected to, "directly coupled to," or "directly contacting" another element, there are no intervening elements present. In the specification, one feature is arranged "adjacent" to another feature, which may mean that one feature has a portion overlapping with the adjacent feature or a portion located above or below the adjacent feature.

In the specification, spatial relationship words such as "upper", "lower", "left", "right", "front", "rear", "high", "low", and the like may describe the relationship of one feature to another feature in the drawings. It will be understood that the spatial relationship words comprise, in addition to the orientations shown in the figures, different orientations of the device in use or operation. For example, when the device in the figures is inverted, features that were originally described as "below" other features may be described as "above" the other features. The device may also be otherwise oriented (rotated 90 degrees or at other orientations) and the relative spatial relationship will be explained accordingly.

The systems described herein may also utilize one or more control modules to receive information and transform the received information to generate an output. The control module may comprise any type of computing device, computing circuitry, or any type of processor or processing circuitry capable of executing a series of instructions stored in the memory module. The control module may include a plurality of processors and/or multi-core Central Processing Units (CPUs) and may include any type of processor, such as microprocessors, digital signal processors, micro-control modules, and the like. The control module may also include a memory module to store data and/or algorithms to execute a series of instructions.

Any method, program, algorithm, or code described in the specification may be converted to or expressed as a programming language or computer program. "programming language" and "computer program" are any language used to assign instructions to a computer, and include, but are not limited to, these languages and their derivatives: assembly language, basic, batch files, BCPL, C, c+, c++, delphi, fortran, java, javaScript, machine code, operating system command language, pascal, perl, PL1, scripting language, visual Basic, meta language itself specifying programs, and first, second, third, fourth, and fifth generation computer languages. Also included are databases and other data schemas, as well as any other meta-languages. For the purposes of this definition, no distinction is made between interpreted, compiled languages, or languages using both compiled and interpreted methods. For the purpose of this definition, no distinction is made between compiled and source versions of the program. Thus, a program that may exist in more than one state (such as a source state, compiled state, object state, or linked state) with reference to a programming language is with reference to any and all such states. The definition also contains valid instructions and the intent of those instructions.

Any of the methods, programs, algorithms, or code described herein may be embodied on one or more machine readable media or storage modules. The term "memory module" may include a mechanism that provides (e.g., stores and/or transmits) information in a machine-readable format, such as a processor, computer, or digital processing device. For example, the memory module may include a read only memory module (ROM), a random access memory module (RAM), a magnetic disk storage medium, an optical storage medium, a flash memory device, or any other volatile or non-volatile memory device. The code or instructions contained thereon may be represented by carrier wave signals, infrared signals, digital signals, and other similar signals.

Fig. 1 shows a schematic diagram of an immunochromatographic strip immediate detection system 1 according to an embodiment of the present disclosure. As shown, the detection system 1 comprises a separate information acquisition device 2 and computing device 3. The information acquisition device 2 and the computing device 3 are separated and communicate with each other by wireless means or by wired means. The information collection device 2 collects various information (e.g., luminescence information, temperature sensing pattern information, identification code information, etc.) from the test strip 4, and transmits the collected information to the computing device 3. The computing device 3 processes the received information and outputs the processing results (e.g., luminescent probe concentration, sample trace data, etc.).

The test strip 4 detected by the detection system 1 is a test strip after receiving the specimen sample for testing. As shown in fig. 2, the test strip 4 may include a cartridge 41 and a nitrocellulose membrane 42 disposed within the cartridge 41. The test strip 4 has a loading port 43 and a display port 44 which are axially spaced apart. The loading port 43 is for receiving a sample (e.g., urine, serum, etc.), and the substance to be detected in the sample and its luminescent conjugates are transferred to the display port 44 by capillary action of the nitrocellulose membrane 42. In some embodiments, the substance to be detected of the immune reaction on the test strip 4 is an antigen, and the luminescent conjugate of the substance to be detected is produced by binding the antigen to an antibody coupled to the surface of the luminescent probe.

The display port 44 includes the nitrocellulose membrane 42 and the T and C lines 45 for test exposed in the display port region, and the transferred substance to be detected and its luminescent conjugate are immunoreacted and immobilized by reaching the T and C lines 45. The display port 44 serves as a light emitting area where the information collecting device 2 collects light emitting information. The display port 44 contains a luminescent probe, such as a fluorescent probe or a long afterglow luminescent probe. Fluorescent probes fluoresce during excitation by excitation light; the long afterglow luminous probe generates afterglow after being excited by excitation light, and the afterglow luminescence can last for more than 100ms after the excitation light is turned off. The luminescence information may be information of fluorescence emitted from the fluorescent probe (for example, luminescence intensity), or information of afterglow emitted from the long afterglow luminescent probe (for example, luminescence intensity).

The identification code 46 is provided on the card case 41 (for example, by printing or pasting) and serves as an area where the information of the identification code is collected by the information collecting device 2. In some embodiments, the identification code 46 may be located on the cartridge 41 on a side of the display port 44 remote from the loading port 43. In some embodiments, the identification code 46 may be a two-dimensional code or a bar code.

The temperature sensing pattern 47 is provided on the card case 41 (for example, by printing or pasting), and serves as an area where the information of the temperature sensing pattern is collected by the information collection device 2. In some embodiments, the temperature sensing pattern 47 may be located on the card housing 41 on a side of the display port 44 away from the loading port 43 and adjacent to the identification code 46. In some embodiments, the temperature sensitive pattern 47 is made of a thermochromic ink, such as a cholesteric liquid crystal temperature sensitive ink. The thermochromic ink has a higher sensitivity in the test temperature range of the test strip 4 and shows different colors at different test temperatures, for example, a temperature change of 1 ℃ is sufficient to produce a recognizable color difference. In some embodiments, the temperature sensing pattern 47 is provided as a plurality of small blocks. Each small block changes color at different critical temperatures due to different material proportions of the thermochromic ink, so that the small blocks can be arranged according to the height sequence of the critical temperatures. For example, the temperature sensing pattern 47 may be rectangular, and the plurality of small blocks are arranged in a plurality of rows and columns; the temperature sensing pattern 47 may be in a straight shape, and a plurality of small blocks are arranged in a row; the temperature sensing pattern 47 may have a ring shape, and a plurality of small blocks are arranged in a ring shape. In some embodiments, the temperature sensing pattern 47 may be provided on the information collection device 2 (e.g., on a support frame or a lower housing of the information collection device 2 as described in detail below) instead of the test strip 4.

Fig. 3 and 4 show an exploded perspective view and an assembled perspective view of the information collection device 2 (with the upper housing removed). As shown in the figure, the information collection device 2 includes an upper case 21 and a lower case 22, and the upper case 21 and the lower case 22 are fitted together to form a light-shielding cavity inside. The support 23 is located in the light-shielding cavity and is fixed to the lower housing 22. The support frame 23 includes a plurality of compartments for supporting one or more components of the collection assembly 24, the communication assembly 25, the power assembly 27, and/or the test strip receiving slot 28. The test strip receiving slot 28 is for receiving the inserted test strip 4. The collection assembly 24 transmits excitation light to the test strip 4 and collects various information (e.g., luminescence information, temperature sensing pattern information, identification code information, etc.) of the test strip 4 during and/or after excitation. The luminescence information comprises fluorescence intensity information of the fluorescent probe or long afterglow luminescence intensity information of the long afterglow luminescence probe respectively. In one embodiment, the luminescence information includes fluorescence intensity information of the fluorescent probe, the light source is in an on state during the fluorescence intensity test, the collection device collects fluorescence intensity information, temperature sensing pattern information and identification code information, preferably three kinds of information are obtained respectively through multiple collection, more preferably three kinds of information are obtained simultaneously at one time. In another embodiment, the luminescence information comprises the long afterglow luminescence intensity information of the long afterglow luminescence probe, preferably the temperature sensing pattern information and the identification code information are collected at the same time of excitation of the excitation light, and the long afterglow luminescence intensity information is collected after the light source is turned off. The communication component 25 transmits the acquired information to the computing device 3. The power supply assembly 27 is used for supplying power to the information acquisition device 2.

The acquisition assembly 24 includes a light source 241, a filter 242, and a camera 243. The light source 241 is used to send excitation light to the test strip 4. The filter 242 serves to filter the background light and the scattered light. The camera 243 captures light emission information of the light emission region, temperature sensing pattern information on the test strip 4, identification code information, and the like by taking a color photograph. In some embodiments, the test strip receiving slot 28 may be disposed opposite the camera 243 and the filter 242; the light source 241 may be disposed offset from the path between the camera 243 and the filter 242, and the test strip receiving slot 28, which are opposite to each other. In some embodiments, the light source 241, the filter 242, and the camera 243 are all placed on the support frame 23. In some embodiments, the light source 241 may be an LED lamp with a light stop. In some embodiments, camera 243 may be a wide angle digital camera, such as a CCD camera or a CMOS camera, or the like.

In some embodiments, when display port 44 comprises a long persistence luminescent probe, filter 242 may comprise a dual filter switch comprised of an infrared cut filter and a full spectrum optical glass, and the dual filter switch may be switched back and forth between the infrared cut filter and the full spectrum optical glass. As shown in fig. 5, the excitation of the long-afterglow luminescent probe is divided into two stages, the first stage being an excitation period in which the light source 241 is turned on to send excitation light to the long-afterglow luminescent probe, and the second stage being an afterglow period in which the long-afterglow luminescent probe emits afterglow after the light source 241 is turned off. During the excitation period, the dual filter switcher switches the filter 242 to an infrared cut filter to attenuate or filter out the high intensity excitation light, thereby protecting the detector of the camera 243 and collecting the identification code information and the temperature sensing pattern information. In the afterglow period, the long afterglow luminescent probe emits afterglow, and the double filter switcher switches the filter 242 to the full spectrum optical glass to collect afterglow information emitted by the long afterglow luminescent probe. In some embodiments, the combination of filter 242 with dual filter switch and camera 243 may be an IR-Cut camera as shown in fig. 6. The IR-Cut camera is a finished lens sold in the market, the selling price is only tens of yuan, and compared with a high-precision camera for collecting and using shorter afterglow in the existing instrument, the cost is reduced by hundreds to thousands of times.

Returning to fig. 3 and 4, the communication component 25 transmits the information acquired by the acquisition component 24 to the computing device 3. The communication component 25 may be a wireless communication device (e.g., WIFI, bluetooth, etc.) or a wired communication device (e.g., through a USB port) for use with a communication component on the computing device 3.

The power supply assembly 27 is used to power the acquisition assembly 24, the communication assembly 25, etc. The power supply assembly 27 may include an internal power source (including a charging pad, lithium battery, etc.), and/or an external power source (e.g., via a USB port).

The test strip receiving slot 28 is fixed to the upper housing 21 or the lower housing 22 and opens on the respective housing for receiving the inserted test strip 4.

In some embodiments, the information acquisition device 2 may be no greater than 10cm in length, no greater than 8cm in width, and no greater than 10cm in height.

Returning to fig. 1, the computing device 3 includes a control module 31, a storage module 32, an output module 33, and a communication module 34. The storage module 32 stores a correspondence relationship between the temperature sensing pattern information and the ambient temperature T0, and stores a plurality of calibration curves L of the light emission intensity of the light emission probe versus the concentration of the light emission probe at a preset temperature. The communication module 34 is a wireless communication module or a wired communication module that mates with the communication module 25. The control module 31 acquires the temperature sensing pattern information and the light emitting information through communication of the communication module 34 and the communication module 25. The control module 31 retrieves the correspondence between the temperature sensing pattern information and the ambient temperature from the storage module 32 to obtain the ambient temperature T0 around the test strip 4. The control module 31 retrieves one or more calibration curves L of luminous intensity of the luminous probe versus concentration of the luminous probe at a preset temperature from the storage module 32 according to the ambient temperature T0. The control module 31 obtains the luminescent probe concentration of the test strip 4 by the luminescent intensity in the luminescent information based on the calibration curve L. The control module 32 outputs the luminescent probe concentration of the test strip 4 through the output module 33.

In some embodiments, the computing device 3 may be a mobile terminal (e.g., a cell phone, a tablet computer, etc.) that is installed with a detection program, or a part of the computing device 3 is provided in the mobile terminal that is installed with a detection program, and another part is provided in a server that communicates with the mobile terminal.

In some embodiments, the control module 31 further obtains identification code information through communication between the communication module 34 and the communication module 25, identifies the trace data of the test strip 4, and stores the trace data to the storage module 32. The output module 33 may output the stored trace data of the test strip 4.

Fig. 7 shows a flow chart of a detection method of the immunochromatographic strip immediate detection system 1. As shown, in step S1, the test strip 4 is inserted into the test strip receiving slot 28 of the information collecting device 2, and a detection command is turned on in the detection program of the computing device 3.

In step S2, the control module 31 of the computing device 3 sends a detection start command to the acquisition component 24 of the information acquisition device 2 through communication between the communication module 34 and the communication component 25. The light source 241 of the collection assembly 24 is turned on and provides excitation light to the test strip 4. In the case where the display port 44 contains a fluorescent probe, the camera 243 takes a color photograph of the test strip 4 during excitation. In the case where the display port 44 includes a long persistence luminescent probe, the filter 242 includes a dual filter switch composed of an infrared cut filter and a full spectrum optical glass. Thus, during the excitation period, the dual filter switcher switches the filter 242 to the infrared cut filter to protect the detector of the camera 243 and take a first color photograph of the test strip 4; during the persistence period, the long persistence luminescent probe emits persistence and the dual filter switch switches the filter 242 to full spectrum optical glass to take a second color photograph of the test strip 4. The first color photograph collecting test strip 4 is provided with temperature sensing pattern information and identification code information, and the second color photograph collecting test strip 4 is provided with luminous information of a display port 44. The color photograph is converted into an electrical signal by the photoelectric signal, and the temperature sensing pattern information and the identification code information, and the light emitting information are outputted to the control module 31 of the computing device 3 through the communication between the communication module 34 and the communication module 25.

In step S3, the control module 31 identifies the trace data of the test strip 4 from the collected identification code information, and outputs the trace data to the storage module 32.

In step S4, the control module 31 retrieves the correspondence between the temperature sensing pattern information and the ambient temperature from the storage module 32, and identifies the ambient temperature T0 around the test strip 4 from the acquired temperature sensing pattern information. The control module 31 includes a function of temperature calibration. The control module 31 determines whether the ambient temperature T0 is equal to a preset temperature corresponding to the calibration curve L. If the ambient temperature T0 is equal to one of the preset temperatures, the control module 31 retrieves a calibration curve L0 of the luminescence probe luminescence intensity versus the luminescence probe concentration at the ambient temperature T0 from the storage module 32. If the ambient temperature T0 is not equal to all the preset temperatures, the control module 31 selects two preset temperatures T1 and T2 adjacent to the ambient temperature T0, and retrieves a calibration curve L1 of the light emitting probe light emitting intensity-light emitting probe concentration at the preset temperature T1 and a calibration curve L2 of the light emitting probe light emitting intensity-light emitting probe concentration at the preset temperature T2 from the storage module 32. The control module 31 obtains the luminescence intensity values of the luminescence probes corresponding to the concentration values of n luminescence probes (n is an integer greater than 1, for example, at least 5) at the preset temperature T1 by using the calibration curve L1, and obtains the luminescence intensity values of the luminescence probes corresponding to the concentration values of n luminescence probes at the preset temperature T2 by using the calibration curve L2. The control module 31 obtains n luminescent probe concentration values and corresponding luminescent probe luminescent intensity values at the ambient temperature T0 from the n luminescent probe concentration values and the corresponding luminescent probe luminescent intensity values at the preset temperatures T1 and T2 by using a certain algorithm (e.g. linear interpolation), thereby modeling a calibration curve L0 of luminescent probe luminescent intensity versus luminescent probe concentration at the ambient temperature T0, for example by linear modeling software, such as using a log-log 4p model or Spline curve Spline function.

The control module 31 fits the light emitting probe concentration according to the calibration curve L0 of the light emitting probe light emitting intensity-light emitting probe concentration at the ambient temperature T0 and the measured light emitting probe light emitting intensity.

In step S5, the output module 33 outputs the luminescent probe concentration and/or the traceability data, for example, for display on a display screen of the computing device 3.

By way of example, the preset temperature may include, for example, 10 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 37 ℃, 39 ℃, 42 ℃, and the like. The luminescence intensities of the luminescence probes at the concentrations of at least 5 luminescence probes are measured at the above preset temperatures, respectively, and the measured points are simulated by linear simulation software to obtain a calibration curve L of luminescence intensity of luminescence probes versus luminescence probe concentration at the above preset temperatures (e.g., including 10 deg.c, 20 deg.c, 25 deg.c, 30 deg.c, 35 deg.c, 37 deg.c, 39 deg.c, and 42 deg.c) and stored in the storage module 32. Thereafter, as shown in fig. 6, the control module 31 retrieves the ambient temperature corresponding to the temperature sensing pattern information from the storage module 32, and obtains the ambient temperature as 27 ℃. The ambient temperature 27 ℃ is not equal to any preset temperature, so the control module 31 selects preset temperatures 25 ℃ and 30 ℃ adjacent to the ambient temperature 27 ℃ and retrieves a calibration curve L1 of the luminous intensity of the luminous probe-the luminous probe concentration at the preset temperature 25 ℃ and a calibration curve L2 of the luminous intensity of the luminous probe-the luminous probe concentration at the preset temperature 30 ℃ from the storage module 32. The control module 31 obtains the luminous intensities of the 6 luminous probes corresponding to the 6 luminous probe concentrations at the preset temperature of 25 ℃ by using the calibration curve L1, and obtains the luminous intensities of the 6 luminous probes corresponding to the 6 luminous probe concentrations at the preset temperature of 30 ℃ by using the calibration curve L2. The control module 31 calculates the luminescence intensities of the 6 luminescence probes corresponding to the concentrations of the 6 luminescence probes at 27 ℃ from the data by using a linear interpolation method, and simulates a calibration curve L0 of the luminescence intensity of the luminescence probes at 27 ℃ through a logarithmic log-log 4p model or Spline curve Spline function. The control module 31 fits the light-emitting probe concentration according to the calibration curve L0 and the measured light-emitting probe light-emitting intensity. The output module 33 displays the luminescent probe concentration and/or the traceability data on a display screen of the computing device 3.

The immunochromatographic test strip instant detection system is high in measurement speed and accurate in measurement.

According to the information acquisition device of the immunochromatographic test strip instant detection system, a processor and a scanning device which occupy a large space are omitted, and the device can be only provided with the size of a palm, so that convenience is greatly improved.

According to the immunochromatographic test strip instant detection system, processing work is completed by a processor in a mobile phone. The mobile phone is also a terminal of future communication, network and information processing core technologies such as 5G, big data, artificial intelligence and the like, and the adoption of the type of terminal is beneficial to directly docking the immunodetection data with the technologies.

The immunochromatographic test strip instant detection system according to the embodiment of the present disclosure adopts thermochromic ink to measure the ambient temperature. The thermochromic ink is sensitive to temperature (the temperature resolution is less than 1 ℃), and can truly, effectively and repeatedly perform temperature identification. The thermochromic ink can be conveniently printed on the test strip, and the process of collecting the temperature sensing pattern information can be carried out simultaneously with the process of collecting the luminous information.

According to the immunochromatographic test strip instant detection system, a plurality of calibration curves of luminous intensity of luminous probes and luminous probe concentration of the test strip to be detected at preset temperature are prestored, and a linear interpolation method and linear simulation software are adopted to obtain the calibration curve at the ambient temperature. According to the actual comparative test, the accuracy of the luminescent probe concentration measurement is remarkably improved.

Although exemplary embodiments of the present disclosure have been described, it will be understood by those skilled in the art that various changes and modifications can be made to the exemplary embodiments of the present disclosure without departing from the spirit and scope of the disclosure. Accordingly, all changes and modifications are intended to be included within the scope of the present disclosure as defined by the appended claims. The disclosure is defined by the following claims, with equivalents of the claims to be included therein.

Claims

1. The detection method for detecting the test strip in real time by using the immunochromatography test strip in real time detection system is characterized by comprising the following steps of:

The information acquisition device transmits excitation light to a test strip comprising a light emitting area with a long afterglow light emitting probe, thereby switching a filter sheet comprising a double-filter switcher consisting of an infrared cut-off filter and full spectrum optical glass to the infrared cut-off filter to protect a camera and taking a first color picture of the test strip in an excitation period of the long afterglow light emitting probe; in the afterglow period of the long afterglow luminous probe, the long afterglow luminous probe emits afterglow, and the filter is switched to the full spectrum optical glass to shoot a second color photo on the test strip, wherein the first color photo acquires temperature sensing pattern information on the test strip, and the second color photo acquires luminous information of a luminous area of the test strip, the luminous information comprises luminous intensity of the luminous area of the test strip, and the temperature sensing pattern is made of thermochromic ink;

the computing device obtains the ambient temperature T0 around the test strip based on the corresponding relation between the preset temperature sensing pattern information and the ambient temperature, and judges whether the ambient temperature T0 is equal to one of a plurality of preset temperatures:

2. The method of claim 1, wherein: the information acquisition device acquires identification code information of the test strip during the period of sending the excitation light, and the computing device identifies tracing data of the test strip from the identification code information and outputs the tracing data of the test strip.

3. The method of claim 1, wherein:

the immunochromatographic test strip instant detection system comprises the information acquisition device and the computing device which are separated from each other, the test strip comprises a light-emitting area, and the test strip and/or the information acquisition device comprises a temperature sensing pattern;

4. A detection method according to claim 3, wherein: the control module obtains n luminous probe luminous intensity values corresponding to n luminous probe concentration values at a preset temperature T1 by utilizing a luminous probe luminous intensity-luminous probe concentration calibration curve L1 at the preset temperature T1, obtains n luminous probe luminous intensity values corresponding to n luminous probe concentration values at the preset temperature T2 by utilizing a luminous probe luminous intensity-luminous probe concentration calibration curve L2 at the preset temperature T2, and obtains the n luminous probe concentration values and the corresponding luminous probe luminous intensity values at an environment temperature T0 by a linear interpolation method from the n luminous probe luminous intensity values and the corresponding n luminous probe luminous intensity values at the preset temperature T1 and the n luminous probe luminous intensity values and the corresponding n luminous probe luminous intensity values at the preset temperature T2, wherein n is an integer greater than 1.

5. The method of claim 4, wherein: the control module simulates a calibration curve L0 of the luminous probe luminous intensity-luminous probe concentration at the ambient temperature T0 by using a logarithmic log-log 4p model or Spline curve Spline function from the n luminous probe concentration values and the corresponding luminous probe luminous intensity values at the ambient temperature T0.

6. The method of claim 1, wherein: the thermochromic ink comprises cholesteric liquid crystal temperature-sensitive ink.

7. The method of claim 1, wherein: the temperature sensing pattern comprises a plurality of adjacent small blocks, and each small block changes color at different critical temperatures due to different material proportions of the temperature sensing color-changing ink.

8. A detection method according to claim 3, wherein: the luminescence information comprises the luminescence intensity of fluorescence emitted by the fluorescent probe on the test strip.

9. A detection method according to claim 3, wherein: the luminous information comprises the luminous intensity of afterglow emitted by a long afterglow luminous probe on the test paper.

10. The detection method according to any one of claims 3 to 9, characterized in that: the card shell of the test strip is provided with an identification code, and the identification code contains traceability data bound with the specimen sample.

11. The method of claim 10, wherein: the acquisition component is configured to also acquire identification code information of the test strip, and the control module identifies traceability data of the test strip from the identification code information.

12. The detection method according to claim 10, wherein: the identification code is a two-dimensional code or a bar code.

13. The detection method according to any one of claims 3 to 9, wherein: the first communication assembly and the second communication module are wireless communication devices or wired communication devices used in cooperation.

14. The detection method according to any one of claims 3 to 9, wherein: the acquisition component comprises a light source, a filter and a camera, wherein the light source is configured to send excitation light to the test strip, and the camera is configured to penetrate the filter to take color pictures of the test strip.

15. The detection method of claim 14, wherein: the test strip is placed opposite the camera and the filter, and the light source is disposed offset from the paths of the camera and the filter, and the test strip opposite each other.

16. The detection method of claim 14, wherein: the light source is an LED lamp with a diaphragm.

17. The detection method of claim 14, wherein: the camera is a wide-angle digital camera.

18. The detection method according to any one of claims 3 to 9, wherein: the information acquisition device comprises an upper shell and a lower shell, and the upper shell and the lower shell are matched together to form a light-proof cavity inside.

19. The detection method of claim 18, wherein: a supporting frame is arranged in the light-shading cavity and fixed on the lower shell.

20. The detection method of claim 19, wherein: the support frame includes a plurality of compartments configured to support a plurality of parts of the information gathering device.

21. The detection method of claim 18, wherein: the information collection device includes a test strip receiving slot that is fixed to the upper housing or the lower housing and that opens at the respective housing to receive an inserted test strip.

22. The detection method according to any one of claims 3 to 9, wherein: the information acquisition device comprises a power supply assembly, and the power supply assembly supplies power to the information acquisition device.

23. The detection method according to any one of claims 3 to 9, wherein: the length of the information acquisition device is not more than 10cm, the width is not more than 8cm, and the height is not more than 10cm.

24. The detection method according to any one of claims 3 to 9, wherein: the computing device is a mobile terminal installed with a detection program, or a part of the computing device is provided in the mobile terminal installed with the detection program, and another part is provided in a server in communication with the mobile terminal.

25. The detection method according to any one of claims 3 to 9, wherein: the test strip comprises a cartridge and a nitrocellulose membrane arranged in the cartridge, wherein the cartridge is provided with a sample loading port and a display port which are axially spaced, and the display port is the light-emitting area.