WO2021138902A1 - Point-of-care testing system for immunochromatographic strip - Google Patents
Point-of-care testing system for immunochromatographic strip Download PDFInfo
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- WO2021138902A1 WO2021138902A1 PCT/CN2020/071459 CN2020071459W WO2021138902A1 WO 2021138902 A1 WO2021138902 A1 WO 2021138902A1 CN 2020071459 W CN2020071459 W CN 2020071459W WO 2021138902 A1 WO2021138902 A1 WO 2021138902A1
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- luminescence
- test strip
- probe
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- 238000012123 point-of-care testing Methods 0.000 title abstract 2
- 239000000523 sample Substances 0.000 claims abstract description 266
- 238000012360 testing method Methods 0.000 claims abstract description 241
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- 239000007850 fluorescent dye Substances 0.000 claims description 8
- 238000003317 immunochromatography Methods 0.000 claims description 6
- 239000004986 Cholesteric liquid crystals (ChLC) Substances 0.000 claims description 5
- 238000004364 calculation method Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Definitions
- the present disclosure relates to the technical field of in vitro diagnosis of medical devices, and in particular to an instant detection system and an instant detection method for immunochromatographic test strips.
- Real-time testing refers to the use of portable instruments by non-professional testing personnel to quickly diagnose and analyze patients' specimen samples. It has the characteristics of convenient carrying, simple operation and on-site inspection. Immunochromatography technology is one of the most important means of real-time detection. The immunochromatography technique detects the fluorescence or afterglow generated by excitation on the test strip, and quantitatively detects the markers on the test strip.
- immunochromatography technology fixes specific antibodies on a certain zone (such as T-line and C-line) of the nitrocellulose membrane.
- a certain zone such as T-line and C-line
- the antigenic substance to be detected in the sample and its luminescent combination with the luminescent probe will act on the nitric acid made from the strip fiber by means of capillary action. Migration on the cellulose membrane.
- the luminescent conjugate of the antigen substance to be detected binds to the specific antibody on the above-mentioned zone, and a specific immune response occurs.
- the zone displays a certain color, or emits a certain intensity of fluorescence or afterglow under the excitation of the excitation light, thereby realizing qualitative or quantitative specific immunodiagnosis.
- the detection instruments used for immunochromatography technology mainly include scanning type detectors and imaging type detectors.
- the scanning detector is composed of an optical module and a mechanical scanning structure.
- the excitation diode of the optical module excites the luminescent probe on the test strip, and the fluorescence generated by the excitation of the luminescent probe is collected by the photodiode and other detectors.
- the detector can only detect the local fluorescence of the test strip at a time, so it is necessary to use the transmission mechanism to drive the optical module or the test strip tray to perform "scanning" detection of the entire test strip. After more than ten seconds, the fluorescence curve is obtained and the sample is calculated. content. Scanning detectors are simple to operate, but the measurement speed is slow, and the measured sample content has a large deviation from the actual value.
- the imaging detector uses a fluorescent imaging camera to take a snapshot of the test strip and then process the image.
- the imaging detector has high requirements on the hardware performance of the operating host, imaging camera and light-emitting diode light source, and has the characteristics of large volume, complicated specimen identification, complicated operation, and professional operation. This type of instrument is mostly used in laboratory occasions and is rarely seen in daily clinical examination occasions.
- ambient temperature is one of the most important parameters of immune response, and the immune calibration curve at each ambient temperature is different.
- the detector in the industry only stores the calibration curve at 37°C, which requires incubating the test environment temperature to 37°C, otherwise the concentration of the luminescent probe obtained by the test will be inaccurate.
- the incubation unit needs to be incubated for nearly half an hour. The incubation unit additionally increases the size and weight of the detector, the operation steps, and the time for each sample measurement.
- One of the objectives of the present disclosure is to provide an instant detection system for immunochromatographic test strips that can overcome at least one defect in the prior art.
- a real-time detection system for immunochromatographic test strips in which:
- the immunochromatographic test strip instant detection system includes an information collection device and a computing device that are separated from each other, the test strip includes a light-emitting area, and the test strip and/or the information collection device includes a temperature sensing pattern;
- the information collection device includes a collection component and a first communication component.
- the collection component sends excitation light to the test strip, collects luminescence information and temperature sensing pattern information of the light-emitting area of the test strip, and the first communication component collects Sending the luminescence information and the temperature sensing pattern information of the luminous area of the test strip to the computing device, where the luminescence information includes the luminous intensity of the luminous area of the test strip;
- the computing device includes a control module, a storage module, a second communication module, and an output module.
- the storage module stores the corresponding relationship between the temperature sensing pattern information and the ambient temperature, and the luminous intensity of the luminescent probe at a plurality of preset temperatures. Needle concentration calibration curve, and the second communication module and the first communication component communicate with each other in a wireless or wired manner,
- control module obtains the light-emitting information and temperature-sensing pattern information of the light-emitting area of the test strip through the communication between the first communication component and the second communication module, and the control module retrieves the temperature-sensing pattern information and the ambient temperature from the storage module. To obtain the ambient temperature T0 around the test strip, and the control module determines whether the ambient temperature T0 is equal to one of the plurality of preset temperatures:
- the control module retrieves the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at the ambient temperature T0 from the storage module;
- the control module selects the preset temperature T1 and the preset temperature T2 adjacent to the ambient temperature T0, and retrieves the preset temperature from the storage module Set the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 at the temperature T1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2, and the control module passes the luminescence at the preset temperature T1 Probe luminescence intensity-luminescence probe concentration calibration curve L1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 simulates the luminescence probe luminescence intensity-luminescence probe concentration calibration at ambient temperature T0 Curve L0;
- the control module fits and obtains the luminescence probe concentration value of the luminescence area of the test strip according to the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 under the ambient temperature T0 and the obtained luminescence information of the luminescence area of the test strip, And output the luminescence probe concentration value of the luminescence area of the test strip through the output module.
- the control module uses the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 at the preset temperature T1 to obtain n at the preset temperature T1
- the n luminescence probe luminescence intensity values corresponding to each luminescence probe concentration value are obtained by using the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 to obtain the n luminescence probes at the preset temperature T2.
- the n luminescence probe luminescence intensity value corresponding to the needle concentration value selects the n luminescence probe concentration values at the preset temperature T1 and the corresponding n luminescence probe luminescence intensity values, and the luminescence intensity values at the preset temperature T2.
- the concentration values of the n luminescent probes and the corresponding luminescence intensity values of the n luminescence probes are linearly interpolated to obtain the concentration values of the n luminescence probes and the corresponding luminescence intensity values of the luminescence probe at the ambient temperature T0 , Where n is an integer greater than 1.
- control module calculates the concentration values of the n luminescent probes and the corresponding luminescence intensity values of the luminescent probes under the ambient temperature T0, by using The logit-log4p model or the Spline function of the spline curve simulates the calibration curve L0 of luminescence probe luminescence intensity-luminescence probe concentration at ambient temperature T0.
- thermosensitive pattern is made of temperature-sensitive color-changing ink.
- thermochromatographic test strips according to clause 4, wherein: the temperature-sensitive color-changing ink includes a cholesteric liquid crystal temperature-sensitive ink.
- thermosensitive pattern includes a plurality of adjacent small blocks, and each small block is different due to the material ratio of the temperature-sensitive color-changing ink. Discoloration at critical temperature.
- the luminescence information includes the luminescence intensity of the fluorescence emitted by the fluorescent probe on the test strip.
- the luminescence information includes the luminescence intensity of the afterglow emitted by the long afterglow luminescence probe on the test strip.
- the collection component is configured to also collect identification code information of the test strip
- the control module recognizes the traceability data of the test strip from the identification code information.
- the identification code is a two-dimensional code or a barcode.
- the acquisition component includes a light source, a filter, and a camera
- the light source is configured to send excitation light to the test strip
- the camera is configured Take a color photo of the test strip through the filter.
- the information collection device includes an upper housing and a lower housing, and the upper housing and the lower housing cooperate to A light-proof cavity is formed inside.
- the support frame includes a plurality of compartments configured to support a plurality of parts of the information collection device.
- the information collection device includes a test strip receiving groove
- the test strip receiving groove is fixed to the upper housing or the lower housing, and in the corresponding The shell has an opening to receive the inserted test strip.
- the computing device is a mobile terminal installed with a detection program, or a part of the computing device is installed in a mobile terminal where the detection program is installed.
- the other part of the terminal is set in a server connected to the mobile terminal.
- test strip includes a card housing and a nitrocellulose membrane arranged in the card housing, and the card housing has axially spaced apart The sample port and the display port, and the display port is the light-emitting area.
- a method for instant detection of test strips using an immunochromatographic test strip instant detection system wherein the method includes the following steps:
- the information collection device sends excitation light to the test strip, and collects luminescence information and temperature sensing pattern information of the light-emitting area of the test strip, the light-emitting information including the luminous intensity of the light-emitting area of the test strip;
- the computing device obtains the luminescence information and the temperature sensing pattern information of the luminous area of the test strip through wireless or wired communication between the information collection device and the computing device;
- the computing device obtains the ambient temperature T0 around the test strip based on the preset corresponding relationship between the temperature sensing pattern information and the ambient temperature, and the computing device determines whether the ambient temperature T0 is equal to one of the plurality of preset temperatures:
- the computing device retrieves the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at the ambient temperature T0;
- the computing device selects the preset temperature T1 and the preset temperature T2 adjacent to the ambient temperature T0, and calls the preset temperature T1
- the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 under the preset temperature T2 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 and the calculation device emits light through the luminescence probe at the preset temperature T1
- the intensity-luminescence probe concentration calibration curve L1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 simulates the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0;
- the calculation device fits the luminescence probe concentration value of the luminescence area of the test strip according to the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0 and the obtained luminescence information of the luminescence area of the test strip, And output the luminescence probe concentration value of the luminescence area of the test strip.
- the ambient temperature T0 around the test strip is obtained, and it is determined whether the ambient temperature T0 is equal to one of the plurality of preset temperatures:
- the ambient temperature T0 is equal to one of the plurality of preset temperatures, call the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at the preset ambient temperature T0;
- the ambient temperature T0 is not equal to any one of the plurality of preset temperatures
- select the preset temperature T1 and the preset temperature T2 adjacent to the ambient temperature T0 and call the preset temperature T1 Luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1, and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2, through the luminescence probe luminescence intensity-luminescence probe luminescence intensity at the preset temperature T1
- the needle concentration calibration curve L1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 simulate the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0;
- the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0 and the obtained luminescence information of the luminescence area of the test strip the luminescence probe concentration value of the luminescence area of the test strip is fitted, and the The concentration value of the luminescence probe in the luminescence area of the test strip is output.
- Luminescence intensity values of the luminescence probes using the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 to obtain n luminescence corresponding to the n luminescence probe concentration values at the preset temperature T2
- the probe luminescence intensity value from the n luminescence probe concentration values and the corresponding luminescence probe luminescence intensity values at the preset temperature T1, and the n luminescence probe concentration values and the corresponding luminescence probe concentration values at the preset temperature T2
- the luminescence intensity value of the luminescence probe is obtained by linear interpolation to obtain the concentration value of the n luminescence probe and the corresponding luminescence intensity value of the luminescence probe under the ambient temperature T0, where n is an integer greater than 1.
- a computing device wherein the computing device includes:
- One or more processors are One or more processors.
- One or more storage modules configured to store a series of computer-executable instructions and computer-accessible data associated with the series of computer-executable instructions
- the one or more processors are caused to perform the method according to any one of clauses 28-31.
- a non-transitory computer-readable storage medium wherein a series of computer-executable instructions are stored on the non-transitory computer-readable storage medium.
- the computing device executes, the one or more computing devices are caused to perform the method described in any one of clauses 28-31.
- An information collection device for instant detection of immunochromatographic test strips wherein the information collection device includes:
- An upper shell and a lower shell, the upper shell and the lower shell cooperate to form a light-proof cavity inside;
- test strip receiving groove is located in the light-proof cavity, and is configured to open on the upper shell or the lower shell to receive the inserted test strip, the test strip includes a long afterglow luminous probe The light-emitting area of the needle;
- the collection component is located in the light-proof cavity and includes a light source for sending excitation light to the inserted test strip, a filter, and a camera for photographing the test strip.
- the filter includes an infrared cut-off filter A dual-filter switcher composed of a full-spectrum optical glass, and the dual-filter switch can switch between an infrared cut filter and a full-spectrum optical glass,
- the dual filter switcher is configured to switch the filter to the infrared cut filter during the excitation period of the long afterglow luminescence probe to filter out high-intensity excitation light, and the camera can pass through the infrared cut filter Take color photos of the test strip; and switch the filter to the full-spectrum optical glass during the afterglow period of the long-lasting luminescence probe, so that the camera can take color photos of the test strip through the full-spectrum optical glass.
- test strip receiving slot is arranged to be opposite to the camera and the filter, and the light source is arranged to be offset from the camera and the filter, and the test strip receiving slot is opposite to each other. path.
- the information collection device further comprises a communication component located in a light-proof cavity, the communication component being configured to send the captured color photo to the information A computing device separated from the collection device.
- thermochromic ink includes a cholesteric liquid crystal thermochromic ink.
- test strip for testing specimen samples, wherein the test strip includes a card shell, and the card shell is provided with a temperature sensing pattern capable of collecting the ambient temperature around the test strip.
- test strip according to clause 49 wherein the temperature-sensitive pattern is made of temperature-sensitive color-changing ink.
- thermochromic ink includes a cholesteric liquid crystal temperature sensitive ink.
- the temperature-sensitive pattern includes a plurality of small blocks, and each small block changes color at a different critical temperature due to a different material ratio of the temperature-sensitive color-changing ink.
- test strip according to clause 52 wherein: the plurality of small pieces are arranged in the order of the critical temperature.
- test strip according to clause 53 wherein: the temperature-sensitive pattern is rectangular, and the plurality of small blocks are arranged in a manner of multiple rows and multiple columns.
- test strip according to clause 53 wherein: the temperature-sensitive pattern is in a straight shape, and the plurality of small blocks are arranged in a row.
- test strip according to clause 53 wherein: the temperature sensing pattern is in a ring shape, and the plurality of small pieces are arranged in a ring shape.
- test strip according to clause 49 wherein the temperature-sensitive pattern is set on the card shell by printing or pasting.
- test strip according to any one of clauses 49-57, wherein: the card housing is also provided with an identification code adjacent to the temperature sensing pattern, and the identification code contains traceability data bound to the specimen sample .
- the identification code is a two-dimensional code or a barcode.
- test strip according to clause 58, wherein: the identification code is set on the card housing by printing or pasting.
- test strip according to any one of clauses 49-57, wherein: the test strip further comprises a nitrocellulose membrane located in the card housing, and the test strip has an axially spaced sample loading port and a display mouth.
- test strip according to clause 61 wherein: the temperature sensing pattern is provided on the card housing on the side of the display port away from the sample loading port.
- test strip according to Clause 61, wherein: the display port includes a nitrocellulose film exposed in the display port area and T-line and C-line for testing.
- Fig. 1 shows a schematic diagram of an instant detection system for immunochromatographic test strips according to an embodiment of the present disclosure
- FIG. 2 shows a front view of a test strip to be detected by the immunochromatographic test strip instant detection system of FIG. 1;
- FIG. 3 and 4 show an exploded perspective view and an assembled perspective view of the information acquisition device of the instant detection system of the immunochromatographic test strip of FIG. 1;
- Figure 5 shows the luminescence characteristic curve of the long afterglow luminescence probe
- Figure 6 shows a perspective view of the IR-Cut camera from different angles
- FIG. 7 shows a flow chart of the detection method of the instant detection system for immunochromatographic test strips of FIG. 1.
- an element when an element is referred to as being “on”, “attached” to another element, “connected” to another element, “coupled” to another element, or “contacting” another element, etc., The element may be directly on another element, attached to another element, connected to another element, coupled to another element, or contacting another element, or an intermediate element may be present. In contrast, it is said that an element is “directly on” another element, “directly attached” to another element, “directly connected” to another element, “directly coupled” to another element, or, or “ When directly contacting another element, there will be no intermediate element.
- a feature when a feature is arranged “adjacent” to another feature, it may mean that a feature has a portion overlapping with an adjacent feature or a portion located above or below the adjacent feature.
- the system described in this specification can also utilize one or more control modules to receive information and transform the received information to generate output.
- the control module may include any type of computing device, computing circuit, or any type of processor or processing circuit capable of executing a series of instructions stored in the storage module.
- the control module may include multiple processors and/or a multi-core central processing unit (CPU) and may include any type of processor, such as a microprocessor, a digital signal processor, a micro-control module, and so on.
- the control module may also include a storage module to store data and/or algorithms to execute a series of instructions.
- Programming language and “computer program” are any language used to assign instructions to a computer, and include (but are not limited to) these languages and their derivatives: assembly language, Basic, batch files, BCPL, C, C+ , C++, Delphi, Fortran, Java, JavaScript, machine code, operating system command language, Pascal, Perl, PL1, scripting language, Visual Basic, the metalanguage of its own designated program, and the first, second, and third generation 1st, 4th and 5th generation computer languages. Also included are databases and other data schemas, as well as any other metalanguage.
- the term "storage module” may include a mechanism for providing (eg, storing and/or transmitting) information in a format readable by a machine such as a processor, computer, or digital processing device.
- the storage module may include a read-only storage module (ROM), a random access storage module (RAM), a magnetic disk storage medium, an optical storage medium, a flash memory device, or any other volatile or non-volatile storage device.
- Fig. 1 shows a schematic diagram of an immunochromatographic test strip instant detection system 1 according to an embodiment of the present disclosure.
- the detection system 1 includes a separate information collection device 2 and a computing device 3.
- the information collection device 2 and the computing device 3 are separated and communicate with each other in a wireless or wired manner.
- the information collection device 2 collects various information (such as luminescence information, temperature-sensing pattern information, identification code information, etc.) from the test strip 4, and sends the collected information to the computing device 3.
- the computing device 3 processes the received information and outputs the processing results (such as the concentration of luminescent probes, sample traceability data, etc.).
- the test strip 4 detected by the detection system 1 is the test strip after receiving the sample for testing.
- the test strip 4 may include a card housing 41 and a nitrocellulose membrane 42 arranged in the card housing 41.
- the test strip 4 has a sample loading port 43 and a display port 44 spaced apart in the axial direction.
- the sample loading port 43 is used to receive a sample (such as urine, serum, etc.), and the substance to be detected and its luminescent combination in the sample are transferred to the display port 44 through the capillary action of the nitrocellulose membrane 42.
- the immunoreactive substance to be detected on the test strip 4 is an antigen
- the luminescent conjugate of the substance to be detected is produced by combining the antigen with the antibody coupled to the surface of the luminescent probe.
- the display port 44 includes the nitrocellulose membrane 42 exposed in the display port area and the T-line and C-line 45 for testing, and an immune response occurs when the delivered substance to be detected and its luminescent conjugate reach the T-line and C-line 45 It is fixed by coupling.
- the display port 44 serves as a light-emitting area for the information collecting device 2 to collect light-emitting information.
- the display port 44 contains a luminescent probe, such as a fluorescent probe or a long-lasting luminescent probe.
- the fluorescent probe generates fluorescence during excitation by the excitation light; while the long-lasting luminescence probe generates afterglow after being excited by the excitation light. After the excitation light is turned off, the afterglow emission can last for more than 100 ms, for example.
- the luminescence information may be information about the fluorescence emitted by the fluorescent probe (e.g., luminescence intensity), or may be information about the afterglow (e.g., luminescence intensity) emitted by the long-lasting luminescence probe.
- the identification code 46 is provided on the card housing 41 (for example, by printing or pasting), and serves as an area where the information collection device 2 collects identification code information.
- the identification code 46 may be located on the card housing 41 on the side of the display port 44 away from the sample loading port 43.
- the identification code 46 may be a two-dimensional code or a barcode.
- the temperature-sensitive pattern 47 is provided on the card housing 41 (for example, by printing or pasting), and serves as an area where the information collecting device 2 collects the temperature-sensitive pattern information.
- the temperature sensing pattern 47 may be located on the card housing 41 on the side of the display port 44 away from the sample loading port 43 and adjacent to the identification code 46.
- the temperature-sensitive pattern 47 is made of a temperature-sensitive color-changing ink, such as a cholesteric liquid crystal temperature-sensitive ink.
- the thermochromic ink has high sensitivity in the test temperature range of the test strip 4, and displays different colors at different test temperatures. For example, a temperature change of 1° C. is sufficient to produce a recognizable color difference.
- the temperature sensing pattern 47 is provided as a plurality of small blocks. Each small block changes color at different critical temperatures due to the different material ratio of the thermochromic ink, so it can be arranged in the order of the height of the critical temperature.
- the temperature-sensitive pattern 47 may be rectangular, and multiple small blocks are arranged in multiple rows and multiple columns; the temperature-sensitive pattern 47 may be in a line shape, and multiple small blocks are arranged in a row; the temperature-sensitive pattern 47 can be in a ring shape, and a plurality of small pieces are arranged in a ring shape.
- the temperature sensing pattern 47 may be provided on the information collection device 2 (for example, on the support frame or lower housing of the information collection device 2 as detailed below) instead of the test strip 4.
- the information collection device 2 includes an upper housing 21 and a lower housing 22, and the upper housing 21 and the lower housing 22 cooperate to form a light-proof cavity inside.
- the support frame 23 is located in a light-proof cavity and fixed on the lower housing 22.
- the support frame 23 includes a plurality of compartments for supporting one or more parts in the collection assembly 24, the communication assembly 25, the power supply assembly 27, and/or the test strip receiving slot 28.
- the test strip receiving slot 28 is used to receive the inserted test strip 4.
- the collection component 24 sends excitation light to the test strip 4, and collects various information (such as luminescence information, temperature sensing pattern information, identification code information, etc.) of the test strip 4 during and/or after the excitation.
- the luminescence information includes the fluorescence intensity information of the fluorescent probe or the long afterglow luminescence intensity information of the long-lasting luminescence probe.
- the luminescence information includes the fluorescence intensity information of the fluorescent probe, the light source is turned on during the fluorescence intensity test, and the collection device collects fluorescence intensity information + temperature sensing pattern information + identification code information, preferably divided into multiple Three kinds of information are obtained in the second collection, and more preferably three kinds of information are obtained at the same time at one time.
- the luminescence information includes the long-lasting luminescence intensity information of the long-lasting luminescence probe.
- the temperature sensing pattern information + identification code information is collected while the excitation light is excited, and the long-lasting luminescence intensity is collected after the light source is turned off. information.
- the communication component 25 sends the collected information to the computing device 3.
- the power supply component 27 is used to supply power to the information collection device 2.
- the collection component 24 includes a light source 241, a filter 242, and a camera 243.
- the light source 241 is used to send excitation light to the test strip 4.
- the filter 242 is used to filter background light and scattered light.
- the camera 243 collects light-emitting information of the light-emitting area, temperature-sensing pattern information and identification code information on the test strip 4 by taking color photos.
- the test strip receiving groove 28 may be arranged to be opposite to the camera 243 and the filter 242; the light source 241 may be arranged to deviate from the camera 243 and the filter 242, and the test strip receiving groove 28 is opposite to each other. route of.
- the light source 241, the filter 242, and the camera 243 are all placed on the support frame 23.
- the light source 241 may be an LED lamp with a diaphragm.
- the camera 243 may be a wide-angle digital camera, such as a CCD camera or a CMOS camera.
- the filter 242 may include a dual filter switch composed of an infrared cut filter and a full-spectrum optical glass, and the dual filter switch The filter can be switched back and forth between the infrared cut filter and the full-spectrum optical glass.
- the long-lasting luminescence probe excitation is divided into two stages. The first stage is the excitation period when the light source 241 is turned on to send excitation light to the long-lasting luminescence probe, and the second stage is the long-lasting luminescence after the light source 241 is turned off. The probe emits an afterglow period of afterglow.
- the dual filter switcher switches the filter 242 to the infrared cut filter to attenuate or filter out the high-intensity excitation light, thereby protecting the detector of the camera 243, and collecting identification code information and temperature sensing Pattern information.
- the long afterglow luminescence probe emits afterglow
- the dual filter switcher switches the filter 242 to the full-spectrum optical glass to collect the afterglow information from the long afterglow luminescence probe.
- the combination of the filter 242 with the dual filter switch and the camera 243 may be the IR-Cut camera shown in FIG. 6.
- the IR-Cut camera is a finished lens sold on the market, and the price is only a dozen yuan. Compared with the high-precision camera used for short afterglow collection in existing instruments, the cost is reduced by hundreds to thousands of times.
- the communication component 25 sends the information collected by the collection component 24 to the computing device 3.
- the communication component 25 may be a wireless communication device (such as WIFI, Bluetooth, etc.) or a wired communication device (such as through a USB port) used in conjunction with the communication component on the computing device 3.
- the power supply component 27 is used to supply power to the collection component 24, the communication component 25, and the like.
- the power supply component 27 may include a built-in power supply (including a charging board, a lithium battery, etc.), and/or an external power supply (for example, through a USB port).
- the test strip receiving groove 28 is fixed to the upper casing 21 or the lower casing 22, and is opened on the corresponding casing for receiving the inserted test strip 4.
- the length of the information collection device 2 may not be greater than 10 cm, the width may not be greater than 8 cm, and the height may not be greater than 10 cm.
- the computing device 3 includes a control module 31, a storage module 32, an output module 33 and a communication module 34.
- the storage module 32 stores the corresponding relationship between the temperature sensing pattern information and the ambient temperature T0, and stores a plurality of calibration curves L of the luminescence intensity of the luminescence probe and the concentration of the luminescence probe at a preset temperature.
- the communication module 34 is a wireless communication module or a wired communication module that cooperates with the communication component 25.
- the control module 31 obtains temperature-sensing pattern information and light-emitting information through the communication between the communication module 34 and the communication component 25.
- the control module 31 retrieves the corresponding relationship between the temperature sensing pattern information and the ambient temperature from the storage module 32 to obtain the ambient temperature T0 around the test strip 4.
- the control module 31 retrieves one or more calibration curves L of the luminescence probe luminescence intensity-luminescence probe concentration at the preset temperature from the storage module 32 according to the ambient temperature T0. Based on the above-mentioned calibration curve L, the control module 31 obtains the luminescence probe concentration of the test strip 4 from the luminescence intensity in the luminescence information.
- the control module 32 outputs the luminescence probe concentration of the test strip 4 through the output module 33.
- the computing device 3 may be a mobile terminal (such as a mobile phone, a tablet computer, etc.) installed with a detection program, or a part of the computing device 3 is installed in a mobile terminal installed with a detection program, and another part is installed in a mobile terminal with a detection program.
- the terminal connected to the server.
- control module 31 also obtains identification code information through the communication between the communication module 34 and the communication component 25, identifies the traceability data of the test strip 4, and stores the traceability data in the storage module 32.
- the output module 33 can output the stored traceability data of the test strip 4.
- FIG. 7 shows a flow chart of the detection method of the instant detection system 1 for immunochromatographic test strips.
- step S1 the test strip 4 is inserted into the test strip receiving slot 28 of the information collection device 2, and the detection command is activated in the detection program of the computing device 3.
- step S2 the control module 31 of the computing device 3 sends a detection start command to the collection component 24 of the information collection device 2 through the communication between the communication module 34 and the communication component 25.
- the light source 241 of the collection component 24 is turned on and provides excitation light for the test strip 4.
- the camera 243 takes a color photo of the test strip 4 during the excitation period.
- the filter 242 includes a dual filter switch composed of an infrared cut filter and a full-spectrum optical glass.
- the dual filter switcher switches the filter 242 to the infrared cut filter to protect the detector of the camera 243 and take the first color photo of the test strip 4; in the afterglow period, the long The afterglow luminous probe emits afterglow, and the dual filter switcher switches the filter 242 to the full-spectrum optical glass to take a second color photo of the test strip 4.
- the first color photo captures the temperature sensing pattern information and identification code information on the test strip 4, and the second color photo captures the light-emitting information of the display port 44 of the test strip 4.
- the color photos are converted into electrical signals by photoelectric signals, and the temperature sensing pattern information, identification code information, and luminescence information are output to the control module 31 of the computing device 3 through the communication between the communication module 34 and the communication component 25.
- step S3 the control module 31 identifies the traceability data of the test strip 4 from the collected identification code information, and outputs the traceability data to the storage module 32.
- step S4 the control module 31 retrieves the corresponding relationship between the temperature sensing pattern information and the ambient temperature from the storage module 32, and recognizes the ambient temperature T0 around the test strip 4 from the collected temperature sensing pattern information.
- the control module 31 includes a temperature calibration function. The control module 31 determines whether the ambient temperature T0 is equal to the preset temperature corresponding to the calibration curve L. If the ambient temperature T0 is equal to one of the plurality of preset temperatures, the control module 31 retrieves the calibration curve L0 of the luminescence probe luminescence intensity-the luminescence probe concentration at the ambient temperature T0 from the storage module 32.
- the control module 31 selects two preset temperatures T1 and T2 adjacent to the ambient temperature T0, and retrieves the preset temperature T1 from the storage module 32
- the control module 31 uses the calibration curve L1 to obtain n (n is an integer greater than 1, for example, at least 5) luminescence probe luminescence intensity values corresponding to the preset temperature T1, and uses the calibration curve L2 to obtain the preset temperature T1.
- the control module 31 uses a certain algorithm (such as linear interpolation) to obtain the n luminescence probe concentration values at the preset temperature T1 and T2 and the corresponding luminescence probe luminescence intensity value to obtain n luminescence probes at the ambient temperature T0.
- the needle concentration value and the corresponding luminescence probe luminescence intensity value thereby simulating the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0, for example, by linear simulation software, such as using a logit-log4p model Or the spline curve Spline function.
- the control module 31 fits and obtains the luminescence probe concentration according to the calibration curve L0 of the luminescence probe luminescence intensity-the luminescence probe concentration under the ambient temperature T0 and the measured luminescence probe luminescence intensity.
- step S5 the output module 33 outputs the luminescent probe concentration and/or traceability data, for example, to display on the display screen of the computing device 3.
- the preset temperature may include 10°C, 20°C, 25°C, 30°C, 35°C, 37°C, 39°C, 42°C, and so on. Measure the luminescence intensity of the luminescence probe at the concentration of at least 5 luminescence probes at the above preset temperature, and simulate the measured points with linear simulation software to obtain the above preset temperature (for example, including 10°C, 20°C, 25°C). , 30°C, 35°C, 37°C, 39°C, 39°C, and 42°C) and store the calibration curve L of the luminescence probe luminescence intensity-luminescence probe concentration at the storage module 32. After that, as shown in FIG.
- the control module 31 retrieves the environment temperature corresponding to the temperature sensing pattern information from the storage module 32, and obtains that the environment temperature is 27°C.
- the ambient temperature of 27°C is not equal to any of the preset temperatures. Therefore, the control module 31 selects the preset temperatures of 25°C and 30°C, which are adjacent to the ambient temperature of 27°C, and retrieves the preset temperature of 25°C from the storage module 32 Lower luminescent probe luminescence intensity-luminescence probe concentration calibration curve L1, luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at a preset temperature of 30°C.
- the control module 31 uses the calibration curve L1 to obtain the luminescence intensities of the 6 luminescent probes corresponding to the 6 luminescent probe concentrations at a preset temperature of 25°C, and uses the calibration curve L2 to obtain the 6 luminescence probe concentrations corresponding to the 6 luminescent probe concentrations at the preset temperature of 30°C.
- the luminescence intensity of a luminescent probe uses the linear interpolation method to calculate the luminescence intensity of the 6 luminescence probes corresponding to the concentration of the 6 luminescence probes at 27°C from the above data, and simulates 27 through the logit-log4p model or the spline function.
- the control module 31 fits and obtains the concentration of the luminescence probe according to the calibration curve L0 and the measured luminescence intensity of the luminescence probe.
- the output module 33 displays the luminescent probe concentration and/or traceability data on the display screen of the computing device 3.
- the instant detection system for immunochromatographic test strips according to the embodiments of the present disclosure has fast measurement speed and relatively accurate measurement.
- the information collection device of the instant detection system for immunochromatographic test strips eliminates the need for a processor and scanning device that occupies a large space, and the device can be only palm-sized, greatly increasing its convenience Sex.
- the processing work is handed over to the processor in the mobile phone to complete.
- Mobile phones are also terminals for future communication, network, and information processing core technologies such as 5G, big data, and artificial intelligence.
- the use of this type of terminal is conducive to directly connecting immune detection data with these technologies.
- thermochromic ink to measure the ambient temperature.
- Thermochromic inks are more sensitive to temperature (the temperature resolution is less than 1°C), and temperature identification can be carried out in a true, effective and repeatable manner.
- the thermochromic ink can be conveniently printed on the test strip, and the process of collecting the temperature-sensitive pattern information can be carried out at the same time as the process of collecting the luminous information.
- the instant detection system for immunochromatographic test strips prestores calibration curves of luminescence probe luminescence intensity-luminescence probe concentration of the test strips to be tested at preset temperatures, and adopts linear interpolation and The linear simulation software obtains the calibration curve at ambient temperature. According to actual comparative experiments, the present disclosure has significantly improved the accuracy of luminescent probe concentration measurement.
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Abstract
Description
Claims (25)
- 一种免疫层析试纸条即时检测系统,其特征在于:A real-time detection system for immunochromatographic test strips, which is characterized in:所述免疫层析试纸条即时检测系统包括彼此分离开的信息采集装置和计算装置,试纸条包括发光区域,试纸条和/或信息采集装置包括感温图案;The immunochromatographic test strip instant detection system includes an information collection device and a computing device that are separated from each other, the test strip includes a light-emitting area, and the test strip and/or the information collection device includes a temperature sensing pattern;信息采集装置包括采集组件和第一通信组件,所述采集组件对试纸条发送激发光,采集试纸条的发光区域的发光信息和感温图案信息,并且所述第一通信组件将采集到的试纸条的发光区域的发光信息和感温图案信息发送至计算装置,所述发光信息包括试纸条的发光区域的发光强度;The information collection device includes a collection component and a first communication component. The collection component sends excitation light to the test strip, collects luminescence information and temperature sensing pattern information of the light-emitting area of the test strip, and the first communication component collects Sending the luminescence information and the temperature sensing pattern information of the luminous area of the test strip to the computing device, where the luminescence information includes the luminous intensity of the luminous area of the test strip;计算装置包括控制模块、存储模块、第二通信模块和输出模块,所述存储模块储存感温图案信息和环境温度之间的对应关系、以及多个预设温度下发光探针发光强度-发光探针浓度校准曲线,并且所述第二通信模块和第一通信组件以无线方式或有线方式彼此连通,The computing device includes a control module, a storage module, a second communication module, and an output module. The storage module stores the corresponding relationship between the temperature sensing pattern information and the ambient temperature, and the luminous intensity of the luminescent probe at a plurality of preset temperatures. Needle concentration calibration curve, and the second communication module and the first communication component communicate with each other in a wireless or wired manner,其中,控制模块通过第一通信组件和第二通信模块之间的连通获得试纸条的发光区域的发光信息和感温图案信息,控制模块从存储模块调取感温图案信息和环境温度之间的对应关系,以得到试纸条周围的环境温度T0,并且控制模块判断环境温度T0与所述多个预设温度之一是否相等:Wherein, the control module obtains the light-emitting information and temperature-sensing pattern information of the light-emitting area of the test strip through the communication between the first communication component and the second communication module, and the control module retrieves the temperature-sensing pattern information and the ambient temperature from the storage module. To obtain the ambient temperature T0 around the test strip, and the control module determines whether the ambient temperature T0 is equal to one of the plurality of preset temperatures:在环境温度T0与所述多个预设温度之一相等的情况下,控制模块从存储模块调取环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0;In the case where the ambient temperature T0 is equal to one of the plurality of preset temperatures, the control module retrieves the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at the ambient temperature T0 from the storage module;在环境温度T0与所述多个预设温度中任一个均不相等的情况下,控制模块选择与环境温度T0上下相邻的预设温度T1与预设温度T2,并从储存模块调取预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1、以及预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2,控制模块通过预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1和预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2模拟出环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0;In the case that the ambient temperature T0 is not equal to any one of the plurality of preset temperatures, the control module selects the preset temperature T1 and the preset temperature T2 adjacent to the ambient temperature T0, and retrieves the preset temperature from the storage module Set the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 at temperature T1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2, and the control module passes the luminescence at the preset temperature T1 Probe luminescence intensity-luminescence probe concentration calibration curve L1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 simulates the luminescence probe luminescence intensity-luminescence probe concentration calibration at ambient temperature T0 Curve L0;控制模块根据环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0和获得的试纸条的发光区域的发光信息,拟合得到试纸条的发光区域的发光探针浓度值,并通过输出模块将试纸条的发光区域的发光探针浓度值输出。The control module fits and obtains the luminescence probe concentration value of the luminescence area of the test strip according to the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 under the ambient temperature T0 and the obtained luminescence information of the luminescence area of the test strip, And output the luminescence probe concentration value of the luminescence area of the test strip through the output module.
- 根据权利要求1所述的免疫层析试纸条即时检测系统,其特征在于:控制模块利用预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1、得到预设温度T1下n个发光探针浓度值对应的n个发光探针发光强度值,利用预设温度T2下的发 光探针发光强度-发光探针浓度校准曲线L2、得到预设温度T2下所述n个发光探针浓度值对应的n发光探针发光强度值,控制模块从预设温度T1下的所述n个发光探针浓度值和对应的n个发光探针发光强度值、和预设温度T2下的所述n个发光探针浓度值和对应的n个发光探针发光强度值,通过线性内插法得到环境温度T0下的所述n个发光探针浓度值和对应的发光探针发光强度值,其中,n为大于1的整数。The immunochromatographic test strip real-time detection system according to claim 1, wherein the control module uses the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 at the preset temperature T1 to obtain the calibration curve L1 at the preset temperature T1. n luminescence probe luminescence intensity values corresponding to n luminescence probe concentration values, use the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 to obtain the n luminescence at the preset temperature T2 The n luminescence probe luminescence intensity value corresponding to the probe concentration value, and the control module starts from the n luminescence probe concentration values at the preset temperature T1 and the corresponding n luminescence probe luminescence intensity values, and the preset temperature T2 The concentration values of the n luminescent probes and the corresponding luminescence intensity values of the n luminescence probes are obtained by linear interpolation to obtain the concentration values of the n luminescence probes and the corresponding luminescence probe luminescence intensity at the ambient temperature T0 Value, where n is an integer greater than 1.
- 根据权利要求2所述的免疫层析试纸条即时检测系统,其特征在于:控制模块从环境温度T0下的所述n个发光探针浓度值和对应的发光探针发光强度值,通过使用对数logit-log4p模型或样条曲线Spline函数模拟出环境温度T0下的发光探针发光强度-发光探针浓度的校准曲线L0。The immunochromatographic test strip instant detection system according to claim 2, wherein the control module obtains the concentration values of the n luminescence probes and the corresponding luminescence intensity values of the luminescence probes at ambient temperature T0 by using The logit-log4p model or the Spline function of the spline curve simulates the calibration curve L0 of the luminescence intensity of the luminescence probe-the concentration of the luminescence probe under the ambient temperature T0.
- 根据权利要求1所述的免疫层析试纸条即时检测系统,其特征在于:感温图案由感温变色油墨制成。The instant detection system for immunochromatographic test strips according to claim 1, wherein the temperature-sensitive pattern is made of temperature-sensitive color-changing ink.
- 根据权利要求4所述的免疫层析试纸条即时检测系统,其特征在于:感温变色油墨包括胆甾型液晶感温油墨。The instant detection system for immunochromatographic test strips according to claim 4, wherein the temperature-sensitive color-changing ink comprises a cholesteric liquid crystal temperature-sensitive ink.
- 根据权利要求4所述的免疫层析试纸条即时检测系统,其特征在于:感温图案包括多个邻接的小块,每个小块因感温变色油墨的材料配比不同而在各自不同的临界温度变色。The instant detection system for immunochromatographic test strips according to claim 4, wherein the temperature sensing pattern includes a plurality of adjacent small blocks, and each small block is different due to the different material ratio of the temperature-sensitive color-changing ink. Discoloration at the critical temperature.
- 根据权利要求1所述的免疫层析试纸条即时检测系统,其特征在于:发光信息包括试纸条上的荧光探针发出的荧光的发光强度。The instant detection system for immunochromatographic test strips according to claim 1, wherein the luminescence information includes the luminescence intensity of the fluorescence emitted by the fluorescent probes on the test strips.
- 根据权利要求1所述的免疫层析试纸条即时检测系统,其特征在于:发光信息包括试纸条上的长余辉发光探针发出的余辉的发光强度。The instant detection system for immunochromatographic test strips according to claim 1, wherein the luminescence information includes the luminescence intensity of the afterglow emitted by the long afterglow luminescence probe on the test strip.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:试纸条的卡壳设有识别码,所述识别码包含与标本样品绑定的溯源数据;The instant detection system for immunochromatographic test strips according to any one of claims 1-8, wherein the card shell of the test strip is provided with an identification code, and the identification code contains traceability data bound to the specimen sample ;和/或,采集组件构造成还采集试纸条的识别码信息,并且控制模块从识别码信息识别试纸条的溯源数据;And/or, the collection component is configured to also collect the identification code information of the test strip, and the control module identifies the traceability data of the test strip from the identification code information;和/或,识别码是二维码或者条形码。And/or, the identification code is a two-dimensional code or a barcode.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:第一通信组件和第二通信模块是配合使用的无线通信器件或者有线通信器件。The instant detection system for immunochromatographic test strips according to any one of claims 1-8, wherein the first communication component and the second communication module are wireless communication devices or wired communication devices that are used in conjunction.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:采集组件包括光源、滤波片和摄像头,光源构造成对试纸条发送激发光,并且摄像头构造成透过滤波片对试纸条拍摄彩色照片;The immunochromatographic test strip instant detection system according to any one of claims 1-8, wherein the collection component includes a light source, a filter and a camera, the light source is configured to send excitation light to the test strip, and the camera Structured to take a color photo of the test strip through the filter;和/或,试纸条放置成与摄像头和滤波片相对,并且光源设置成偏离摄像头和滤 波片、以及试纸条之间的彼此相对的路径;And/or, the test strip is placed opposite to the camera and the filter, and the light source is set to deviate from the path between the camera, the filter, and the test strip opposite to each other;和/或,光源为带有光阑的LED灯;And/or, the light source is an LED lamp with a diaphragm;和/或,摄像头为广角数字摄像头。And/or, the camera is a wide-angle digital camera.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:信息采集装置包括上壳体和下壳体,上壳体和下壳体配合在一起以在内部形成避光的空腔;The instant detection system for immunochromatographic test strips according to any one of claims 1-8, wherein the information collection device comprises an upper housing and a lower housing, and the upper housing and the lower housing cooperate to A light-proof cavity is formed inside;和/或,避光空腔内设置有支撑架,并且支撑架固定在下壳体上;And/or, a support frame is arranged in the light-proof cavity, and the support frame is fixed on the lower shell;和/或,支撑架包括多个隔层,所述多个隔层构造成支撑信息采集装置的多个零件;And/or, the support frame includes a plurality of compartments configured to support a plurality of parts of the information collection device;和/或,信息采集装置包括试纸条接收槽,所述试纸条接收槽固定至上壳体或下壳体,并且在相应壳体上开口,以接收插入的试纸条。And/or, the information collection device includes a test strip receiving groove, the test strip receiving groove is fixed to the upper casing or the lower casing and opens on the corresponding casing to receive the inserted test strip.
- 根据权利要求1-8中任一项所述的荧光免疫层析试纸条即时检测系统,其特征在于:信息采集装置包括电源组件,所述电源组件对信息采集装置供电。The instant detection system for fluorescence immunochromatographic test strips according to any one of claims 1-8, wherein the information collection device comprises a power supply component, and the power supply component supplies power to the information collection device.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:信息采集装置的长度不大于10cm,宽度不大于8cm,高度不大于10cm。The instant detection system for immunochromatographic test strips according to any one of claims 1-8, wherein the length of the information collection device is not greater than 10 cm, the width is not greater than 8 cm, and the height is not greater than 10 cm.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:计算装置是安装有检测程序的移动终端,或者计算装置的一部分设置在安装有检测程序的移动终端中、另一部分设置在与移动终端连通的服务器中。The instant detection system for immunochromatographic test strips according to any one of claims 1-8, wherein the computing device is a mobile terminal installed with a detection program, or a part of the computing device is installed in a device where the detection program is installed. The other part of the mobile terminal is set in a server connected to the mobile terminal.
- 根据权利要求1-8中任一项所述的免疫层析试纸条即时检测系统,其特征在于:试纸条包括卡壳和设置在卡壳内的硝酸纤维素膜,卡壳具有沿轴向间隔开的上样口和显示口,显示口为所述发光区域。The instant detection system for immunochromatographic test strips according to any one of claims 1-8, wherein the test strip comprises a card housing and a nitrocellulose membrane arranged in the card housing, and the card housing has axially spaced apart The sample loading port and display port of, the display port is the light-emitting area.
- 一种利用免疫层析试纸条即时检测系统对试纸条进行即时检测方法,其特征在于,所述方法包括以下步骤:A method for instant detection of test strips using an immunochromatographic test strip instant detection system, characterized in that the method includes the following steps:将试纸条插入信息采集装置中,并且在计算装置中开启检测命令;Insert the test strip into the information collection device, and start the detection command in the computing device;信息采集装置对试纸条发送激发光,并采集试纸条的发光区域的发光信息和感温图案信息,所述发光信息包括试纸条的发光区域的发光强度;The information collection device sends excitation light to the test strip, and collects luminescence information and temperature sensing pattern information of the light-emitting area of the test strip, the light-emitting information including the luminous intensity of the light-emitting area of the test strip;计算装置通过信息采集装置和计算装置之间的无线方式连通或有线方式连通获得试纸条的发光区域的发光信息和感温图案信息;The computing device obtains the luminescence information and the temperature sensing pattern information of the luminous area of the test strip through wireless or wired communication between the information collection device and the computing device;计算装置基于预设的感温图案信息和环境温度之间的对应关系,得到试纸条周围的环境温度T0,并且计算装置判断环境温度T0与所述多个预设温度之一是否相等:The computing device obtains the ambient temperature T0 around the test strip based on the preset corresponding relationship between the temperature sensing pattern information and the ambient temperature, and the computing device determines whether the ambient temperature T0 is equal to one of the plurality of preset temperatures:在环境温度T0与所述多个预设温度之一相等的情况下,计算装置调取环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0;In the case that the ambient temperature T0 is equal to one of the plurality of preset temperatures, the calculation device retrieves the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at the ambient temperature T0;在环境温度T0与所述多个预设温度中任一个均不相等的情况下,计算装置选择与环境温度T0上下相邻的预设温度T1与预设温度T2,并调取预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1、以及预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2,计算装置通过预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1和预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2模拟出环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0;In the case that the ambient temperature T0 is not equal to any one of the plurality of preset temperatures, the computing device selects the preset temperature T1 and the preset temperature T2 adjacent to the ambient temperature T0, and calls the preset temperature T1 The luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 under the preset temperature T2 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2, and the calculation device emits light through the luminescence probe at the preset temperature T1 The intensity-luminescence probe concentration calibration curve L1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 simulates the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0;计算装置根据环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0和获得的试纸条的发光区域的发光信息,拟合得到试纸条的发光区域的发光探针浓度值,并将试纸条的发光区域的发光探针浓度值输出。The calculation device fits the luminescence probe concentration value of the luminescence area of the test strip according to the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0 and the obtained luminescence information of the luminescence area of the test strip, And output the luminescence probe concentration value of the luminescence area of the test strip.
- 根据权利要求17所述的检测方法,其特征在于:信息采集装置在发送激发光期间采集试纸条的识别码信息,并且计算装置从识别码信息识别试纸条的溯源数据,并将试纸条的溯源数据输出。The detection method according to claim 17, wherein the information collecting device collects the identification code information of the test strip during the period of sending the excitation light, and the computing device recognizes the traceability data of the test strip from the identification code information, and integrates the test strip The traceability data output of the article.
- 根据权利要求17或18所述的检测方法,其特征在于:所述免疫层析试纸条即时检测系统是根据权利要求1-24中任一项所述的免疫层析试纸条即时检测系统。The detection method according to claim 17 or 18, wherein the instant detection system for immunochromatography test strips is the instant detection system for immunochromatography test strips according to any one of claims 1-24 .
- 一种免疫层析试纸条即时检测方法,其特征在于,所述方法包括以下步骤:An instant detection method for immunochromatographic test strips, characterized in that the method comprises the following steps:接收试纸条的发光区域的发光信息和感温图案信息;Receiving the light-emitting information and temperature-sensing pattern information of the light-emitting area of the test strip;基于预设的感温图案信息和环境温度之间的对应关系,得到试纸条周围的环境温度T0,并且判断环境温度T0与多个预设温度之一是否相等:Based on the corresponding relationship between the preset temperature sensing pattern information and the ambient temperature, the ambient temperature T0 around the test strip is obtained, and it is determined whether the ambient temperature T0 is equal to one of the plurality of preset temperatures:在环境温度T0与所述多个预设温度之一相等的情况下,调取预设的环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0;In the case that the ambient temperature T0 is equal to one of the plurality of preset temperatures, call the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at the preset ambient temperature T0;在环境温度T0与所述多个预设温度中任一个均不相等的情况下,选择与环境温度T0上下相邻的预设温度T1与预设温度T2,并调取预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1、以及预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2,通过预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1和预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2模拟出环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0;In the case that the ambient temperature T0 is not equal to any one of the plurality of preset temperatures, select the preset temperature T1 and the preset temperature T2 adjacent to the ambient temperature T0, and call the preset temperature T1 Luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1, and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2, through the luminescence probe luminescence intensity-luminescence probe luminescence intensity at the preset temperature T1 The needle concentration calibration curve L1 and the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 simulate the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0;根据环境温度T0下的发光探针发光强度-发光探针浓度校准曲线L0和获得的试纸条的发光区域的发光信息,拟合得到试纸条的发光区域的发光探针浓度值,并将试 纸条的发光区域的发光探针浓度值输出。According to the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L0 at ambient temperature T0 and the obtained luminescence information of the luminescence area of the test strip, the luminescence probe concentration value of the luminescence area of the test strip is fitted, and the The concentration value of the luminescence probe in the luminescence area of the test strip is output.
- 根据权利要求20所述的检测方法,其特征在于:利用预设温度T1下的发光探针发光强度-发光探针浓度校准曲线L1、得到预设温度T1下n个发光探针浓度值对应的n个发光探针发光强度值,利用利用预设温度T2下的发光探针发光强度-发光探针浓度校准曲线L2、得到预设温度T2下所述n个发光探针浓度值对应的n个发光探针发光强度值,从预设温度T1下的所述n个发光探针浓度值和对应的发光探针发光强度值、和预设温度T2下的所述n个发光探针浓度值和对应的发光探针发光强度值,通过线性内插法得到环境温度T0下的所述n个发光探针浓度值和对应的发光探针发光强度值,其中,n为大于1的整数。The detection method according to claim 20, characterized in that: using the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L1 at a preset temperature T1 to obtain n luminescence probe concentration values corresponding to the preset temperature T1 n luminescence probe luminescence intensity values, using the luminescence probe luminescence intensity-luminescence probe concentration calibration curve L2 at the preset temperature T2 to obtain n corresponding to the n luminescence probe concentration values at the preset temperature T2 The luminescence intensity value of the luminescence probe is calculated from the n luminescence probe concentration values and the corresponding luminescence probe luminescence intensity values at the preset temperature T1, and the n luminescence probe concentration values at the preset temperature T2 and The corresponding luminescence probe luminescence intensity value is obtained by linear interpolation to obtain the n luminescence probe concentration values and the corresponding luminescence probe luminescence intensity value at ambient temperature T0, where n is an integer greater than 1.
- 根据权利要求20所述的检测方法,其特征在于:从环境温度T0下的所述n个发光探针浓度值和对应的n个发光探针发光强度值,通过使用对数logit-log4p模型或样条曲线Spline函数模拟出环境温度T0下的发光探针发光强度-发光探针浓度的校准曲线L0。The detection method according to claim 20, characterized in that: from the n luminescent probe concentration values and the corresponding n luminescent probe luminescence intensity values at ambient temperature T0, by using a logit-log4p model or The Spline function of the spline curve simulates the calibration curve L0 of the luminescence intensity of the luminescence probe-the concentration of the luminescence probe under the ambient temperature T0.
- 根据权利要求20-22中任一项所述的检测方法,其特征在于:接收试纸条的识别码信息,并且从识别码信息识别试纸条的溯源数据,并将试纸条的溯源数据输出。The detection method according to any one of claims 20-22, wherein the identification code information of the test strip is received, and the traceability data of the test strip is identified from the identification code information, and the traceability data of the test strip is identified Output.
- 一种计算装置,其特征在于,所述计算装置包括:A computing device, characterized in that the computing device includes:一个或多个处理器;以及One or more processors; and一个或多个存储模块,所述一个或多个存储模块被配置为存储一系列计算机可执行的指令以及与所述一系列计算机可执行的指令相关联的计算机可访问的数据,One or more storage modules configured to store a series of computer-executable instructions and computer-accessible data associated with the series of computer-executable instructions,其中,当所述一系列计算机可执行的指令被所述一个或多个处理器执行时,使得所述一个或多个处理器进行如权利要求20-23中任一项所述的方法。Wherein, when the series of computer-executable instructions are executed by the one or more processors, the one or more processors are caused to perform the method according to any one of claims 20-23.
- 一种非临时性计算机可读存储介质,其特征在于,所述非临时性计算机可读存储介质上存储有一系列计算机可执行的指令,当所述一系列计算机可执行的指令被一个或多个计算装置执行时,使得所述一个或多个计算装置进行如权利要求20-23中任一项所述的方法。A non-transitory computer-readable storage medium, characterized in that a series of computer-executable instructions are stored on the non-transitory computer-readable storage medium, and when the series of computer-executable instructions is replaced by one or more When executed by the computing device, the one or more computing devices are caused to perform the method according to any one of claims 20-23.
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