CN208833768U - Optical signal detecting device and system - Google Patents

Abstract

The utility model provides a kind of optical signal detecting device and system.The optical signal detecting device includes: at least one light source, is arranged above the reaction zone of the reaction carriers after reacting with determinand and is irradiated；Imaging sensor is arranged at the Image Acquisition feeder connection of at least one light source, for acquiring the image of the reaction carriers in the reaction zone；And image pre-processor, it is electrically connected with described image sensor, is pre-processed for the image to the reaction carriers collected.Using the device, the data volume that optical signal detecting device is sent to outside can be substantially reduced.

Description

Optical signal detecting device and system

Technical field

The utility model is usually directed to predetermined substance detection field, reacts more particularly, to for detecting with determinand The optical signal detecting device and system of the optical signal value of the reaction zone of reaction carriers afterwards.

Background technique

The problems such as people increasingly pay attention to the quality of life in recent years, and health indicator declines, food safety, pesticide residue Exposure rate is high, and the universal of Rapid detection test strip allows what people can be convenient to obtain the qualitative of some health indicators etc. As a result, however most test paper are to take visual results, and these qualitative results have been unable to meet public needs, urgent need hair Bright a kind of test paper quantitative testing device that can be used for family's detection, so that the content of the related determinand in test sample, right It is monitored in the problems such as food safety, pesticide residue, to select more safe and healthy life style according to result.

It is to be irradiated after tracer reaction occurs in certain light source currently, carrying out the principle of quantitative analysis to test paper Under, reaction zone is scanned by sensor, intensity curves is obtained and carries out data analysis, or obtained by imaging sensor The image of test paper reaction zone takes pictures to test paper reaction zone, carry out data processing according to image, finally obtain concentration numbers According to.

Currently, it is in the prior art based on the test strips quantitative testing device taken pictures, substantially it is divided to two kinds:

One is the instruments for directly calculating concentration results, and as disclosed in CN105911268A, instrument includes image Acquisition unit, control unit (control and calculate work), unit as the result is shown, carry out whole processing of image until calculate to Survey the concentration of object.The advantages of this instrument is can to immediately arrive at desired concentration results, the disadvantage is that instrument itself is more complicated, A possibility that volume is larger, higher cost, develops into family's detection device is smaller.

One is using disclosed in mobile phone photograph, such as CN 106645706A, do not need equipment, can be obtained as a result, After taking pictures to detection zone, whole picture is dealt into cloud processor, whole data processings is carried out by cloud processor. Taking pictures mainly to be faced with is influenced by different mobile phone models, brand, and effect of taking pictures has significant difference, and due in difference Effect of taking pictures under the influence of external light source has biggish difference.It is widely different thus to cause testing result.

There are also be directly uploaded to internet progress data processing, this side after one is taking pictures using instrument, acquiring image The advantages of method is that comparison is accurate, and cost is relatively low.However this method is there is also disadvantage, and first, since whole image being uploaded, Volume of transmitted data is big, is easily lost, and extends detection time, not convenient and fast.To receiving and processing the upper of picture It is confidential to ask configuration higher, need the image-capable based on high level operating system to handle image.Instrument or hand simultaneously Ability of the camera of machine itself without processing picture, it is necessary to original image data is transferred to host computer, in turn resulted in biography Transmission of data amount is excessive, and extends detection time, especially under the application scenarios of low communication speed, transfers one and is able to satisfy The image data for detecting minimum requirements needs nearly 1 minute or so time.And since instantaneous transmission data volume is big, inevitably passing Lost part data during defeated in turn result in detection failure.Second, since current camera design principle is all real-time acquisition figure Picture, when the reaction time as defined in candidate agent reaches, the image that host computer saves the time point is analyzed and is handled.In this mistake Cheng Zhong, camera and interlock circuit in host computer and instrument are all it is larger to cause complete machine fever, and consume in working always Electrode is high, cannot achieve portable.

In addition, in entire detection process, host computer must be always for the optical signal detecting device of not buffer storage It connects camera and acquires image, but only retain the picture of specified time, cause overall power, the hair of optical signal detecting device Heat is all significantly raised, while wasting a large amount of electric energy.Since host computer must be connected to always camera, also corresponding limitation The application scenarios of instrument.

Utility model content

In view of above-mentioned, the utility model provides a kind of optical signal detecting device and system.It is filled using the optical signal detecting It sets and system, the data volume that optical signal detecting device is sent to outside can be substantially reduced.

One aspect according to the present utility model provides a kind of optical signal detecting device, comprising: at least one light source, It is arranged to the reaction zone of the reaction carriers after irradiation is reacted with determinand；Imaging sensor is arranged at least one described light At the Image Acquisition feeder connection in source, for acquiring the image information of the reaction carriers in the reaction zone；And image Preprocessor is electrically connected with described image sensor, is located in advance for the image information to the reaction carriers collected Reason.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: buffer, if It sets between described image sensor and described image preprocessor, for caching the image of the reaction carriers collected.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: timer, with Described image sensor electrical connection, for timing and when the timing expires, triggering described image sensor carries out image Acquisition.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: parameter setting list Member is electrically connected with the timer, for the property according to the marker on predetermined substance to be measured and the reaction carriers, setting Image Acquisition time started, Image Acquisition deadline and the Image Acquisition gap periods of described image sensor；And with The timing trigger unit of the timer electrical connection, it is described fixed for being triggered after the reaction carriers enter detection ready state When device start timing.

Optionally, in an example of above-mentioned aspect, described image preprocessor may include: image interception module, It is electrically connected with described image sensor, for being carried to the reaction collected according to pre-set image acquisition window location parameter The image of body carries out intercept operation；And image compression module, it is electrically connected with described image interception module, for being intercepted The image information of the reaction carriers carries out image Compression.

Optionally, in an example of above-mentioned aspect, at least one described light source is arranged to react from determinand It is irradiated above the reaction zone of reaction carriers afterwards.

Optionally, in an example of above-mentioned aspect, at least one described light source is arranged in reacted with determinand after The top of the reaction zone of reaction carriers.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: light path converting dress Set, be arranged between at least one described light source and the reaction zone of the reaction carriers, for at least one described light source into Row light path converting above the reaction zone of the reaction carriers to be irradiated.

Optionally, in an example of above-mentioned aspect, at least one described light source is fluorescence excitation light source and described Imaging sensor has and the matched light-filtering characteristic of wavelength of fluorescence that is excited.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: one or more Optical filter, is separately positioned between the light source and/or described image sensor and the reaction carriers and and specific wavelength Matching.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: temperature sensing Device, for detecting the temperature data when the reaction carriers are in detecting state.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: communication unit, Described image preprocessor is set between the content determination unit that is arranged in host computer or cloud server, for described Data communication between image pre-processor and the content determination unit.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: camera lens, setting Between described image sensor and the reaction zone of the reaction carriers.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: controller, with In at least one described light source, described image sensor and/or described device other components electrical connection, for it is described at least The irradiation of one light source, the Image Acquisition of imaging sensor, the data communication between the various components in described device and/or institute The data communication stated between component and outside in device is controlled.

Optionally, in an example of above-mentioned aspect, the various components in the optical signal detecting device are arranged in together On one printed circuit board.

Optionally, in an example of above-mentioned aspect, the optical signal detecting device can also include: structural support Part, the various components being used to support in the optical signal detecting device；Shell；And/or setting is in reaction carriers and described Shading piece some or all of in various components between component.

Optionally, in an example of above-mentioned aspect, the structural support is provided with to be carried for accommodating the reaction The reaction carriers accommodation space of body；And/or the structural support is provided with the battery accommodating space for accommodating battery.

Another aspect according to the present utility model, provide a kind of content of predetermined substance detection in determinand is System, which is characterized in that the system comprises: reaction carriers；Optical signal detecting device as described above；And content determines list Member is electrically connected with the optical signal detecting device, for the image based on the pretreated reaction carriers, determines specific object The content of matter.

Optionally, in an example of above-mentioned aspect, the system can also include: host computer, true with the content Order member and optical signal detecting device electrical connection, for content of predetermined substance testing result to be presented to user and/or to institute State the operation of optical signal detecting device, the operation of the content determination unit and described device and the content determination unit it Between operation controlled.

Optionally, in an example of above-mentioned aspect, content determination unit setting beyond the clouds server or on In the machine of position.

Optionally, in an example of above-mentioned aspect, the reaction carriers are reagent strips, and the reagent strip includes bottom plate And successively sequence overlap joint pastes such as lower component: i) sample pad, ii on bottom plate) sample bonding pad, wherein the sample combines The top of pad is further coated with antibody layer, wherein ii-a) antibody layer include marker conjugation anti-quinolone first Antibody, marker conjugation tetracycline resistance secondary antibody and marker conjugation anti-sulfanilamide (SN) third antibody and optional deposit The 4th antibody as Quality Control, iii) example reaction pad, wherein being provided with 3 detection reaction zones on the example reaction pad With 1 Quality Control reaction zone, wherein iii-a) it is described detection reaction zone be respectively be coated with quinolone first detection reaction zone, It is coated with the second detection reaction zone of tetracycline, and is coated with the third detection reaction zone and iii-b of sulfanilamide (SN)) Quality Control is anti- Area is answered to be fixed with antigen or antibody as Quality Control, iv) water absorption pad.

Optionally, in an example of above-mentioned aspect, 4 are further provided on the example reaction pad each other Every enclosed area, and 3 detection reaction zones and 1 Quality Control reaction zone are coated on the top of each enclosed area respectively.

Optionally, in an example of above-mentioned aspect, a length of 15-25mm of the sample pad, width 2-6mm, thickness For 0.5-1.0mm.

Optionally, in an example of above-mentioned aspect, a length of 20 ± 1mm of the sample pad, width is 4 ± 1mm, thickness For 0.7 ± 0.1mm.

Optionally, in an example of above-mentioned aspect, a length of 5-10mm of the sample bonding pad, width 2-6mm are thick Degree is 0.5-1.0mm.

Optionally, in an example of above-mentioned aspect, a length of 8 ± 1mm of the sample bonding pad, width is 4 ± 1mm, With a thickness of 0.7 ± 0.1mm.

Optionally, in an example of above-mentioned aspect, a length of 20-40mm of the water absorption pad, width 2-6mm, thickness For 0.5-1.0mm.

Optionally, in an example of above-mentioned aspect, a length of 31 ± 1mm of the water absorption pad, width is 4 ± 1mm, thickness For 0.7 ± 0.1mm.

Optionally, in an example of above-mentioned aspect, the spacing distance between 4 enclosed areas is identical or not With.

Optionally, in an example of above-mentioned aspect, the spacing distance between 4 enclosed areas is 3 ± 1mm.

Optionally, in an example of above-mentioned aspect, the marker is colloidal gold or fluorescent microsphere.

Optionally, in an example of above-mentioned aspect, the quinolone is selected from one or more of: Ciprofloxacin, Norfloxacin, Enrofloxacin and Ofloxacin；The tetracycline is selected from one or more of: tetracycline, terramycin, aureomycin And fortimicin；And the sulfanilamide (SN) is selected from one or more of: sulphadiazine and sulfamethazine.

Optionally, in an example of above-mentioned aspect, the antibody layer does not include the 4th antibody as Quality Control.

Optionally, in an example of above-mentioned aspect, the quinolone, tetracycline and sulfanilamide (SN) are present in selected from the following In sample: meat and its products, milk and its product, eggs and its product, human body fluid sample.

Optionally, in an example of above-mentioned aspect, the reaction carriers are test cards, and the test card includes: i) Test strips as described above and ii) cladding test strips as described above shell, wherein the shell includes: ii-1) be provided with The Ka Gai of well and detection hole, wherein the well is opened on the upside of the sample pad, to expose the sample pad Partial region, the detection hole is opened on the upside of the example reaction pad, to expose the detection reaction zone and described The whole region and ii-2 of Quality Control reaction zone) and the pedestal that is connected to each other of the Ka Gai.

Detailed description of the invention

By referring to following attached drawing, may be implemented to further understand the nature and advantages of present disclosure.? In attached drawing, similar assembly or feature can have identical appended drawing reference.

Fig. 1 shows the box of the system according to the present utility model for the content of predetermined substance detection in determinand Figure；

Fig. 2 shows the schematic diagrames of test paper according to the present utility model；

Fig. 3 shows the block diagram of the optical signal detecting device of first embodiment according to the present utility model；

The specific implementation that Fig. 4 A shows the optical signal detecting device of second embodiment according to the present utility model is shown The outer shape schematic diagram of example；

Fig. 4 B-4E shows the exemplary internal structure chart of specific implementation shown in Fig. 4 A；

Fig. 5 A and Fig. 5 B show one of the optical signal detecting device of 3rd embodiment according to the present utility model specifically The structural schematic diagram of implementation example；

Fig. 6 shows the side view of the test strips of fourth embodiment according to the present utility model；With

Fig. 7 shows the top view of the test card of fourth embodiment according to the present utility model.

Specific embodiment

Theme described herein is discussed referring now to example embodiment.It should be understood that discussing that these embodiments are In order to enable those skilled in the art can better understand that being not to claims to realize theme described herein Middle illustrated protection scope, applicability or exemplary limitation.It can be in the feelings for the protection scope for not departing from present disclosure Under condition, the function and arrangement of the element discussed are changed.Each example can according to need, and omits, substitutes or adds Add various processes or component.For example, described method can be executed according to described order in a different order, and Each step can be added, omits or combine.In addition, feature described in relatively some examples in other examples may be used To be combined.

As used in this article, term " includes " and its modification indicate open term, are meant that " including but not limited to ". Term "based" indicates " being based at least partially on ".Term " one embodiment " and " embodiment " expression " at least one implementation Example ".Term " another embodiment " expression " at least one other embodiment ".Term " first ", " second " etc. may refer to not Same or identical object.Here may include other definition, either specific or implicit.Unless bright in context It really indicates, otherwise the definition of a term is consistent throughout the specification.

Herein, term " connected ", which refers to, is directly mechanically connected, is connected to or is electrically connected between two components, or passes through Intermediate module comes direct mechanical connection, connection or electrical connection.Term " electrical connection " refers to that electricity can be carried out between two components to be led to Letter is to carry out data/information exchange.Equally, the electrical connection can refer to directly is electrically connected between two components, or passes through Between component come be electrically connected indirectly.The electrical connection can be realized using wired mode or wireless mode.

In the present invention, term " T line " refers to the detection line of reaction carriers, namely detection reaction zone；And term " C line " refers to the nature controlling line namely Quality Control reaction zone of reaction carriers.

In the present invention, detection test paper or reagent can be formed significantly after tracer reaction occurs in reaction zone Change in optical signal.Such as when detection colloid gold test paper, when light source irradiates the test paper after reacting, test paper reaction zone is after reacting Stronger absorption can be formed to incident light, and other regions are generally reflected light, according to langbobier law, the intensity of absorption with There are linear relationships for the concentration of determinand, it can thus be derived that, the concentration existence function relationship for the gray scale and determinand of developing the color.

Therefore, test paper or examination in test paper or reagent quantitative detection process, after being reacted using specific light source irradiation tracer Agent, image sensor senses and the image information for obtaining test paper or reagent, described image information include colouring information and gray scale letter Breath, wherein it is much of that as long as grayscale information for colloidal gold or fluorescent test paper, and certain drying chemical reagent papers are detected, it needs Colouring information.Then data processing is carried out to extract optical signal detecting value to the image information, then substitutes into optical signal detecting Value and testing concentration relation curve, obtain the concentration value of determinand.

Fig. 1 shows the system according to the present utility model for the content of predetermined substance detection in determinand (hereinafter Referred to as content of predetermined substance detection system) block diagram.

As shown in fig. 1, content of predetermined substance detection system includes reaction carriers 10.Reaction carriers 10 have reaction zone The non-reaction zone and.The label that being equipped in the reaction zone of reaction carriers 10 can react with predetermined substance to be determined reacts Object.The labeling reaction is not provided in the non-reaction zone of reaction carriers 10.Since the principles of the present invention are detection lights Signal value, it is all react with determinand after generate change in optical signal reaction carriers can be applicable in, reaction carriers 10 Example for example may include the test paper or reagent card of any detection macromolecular or small molecule chemicals, include but is not limited to take Test paper prepared by competition law, sandwich method principle.The example of the test paper for example may include colloid gold test paper, and fluorescent test paper is done Chemical test paper etc..The example of reagent card for example may include micro-fluidic reagent card etc..Fig. 2 shows according to the present utility model The schematic diagram of test paper.In test paper shown in Fig. 2,2 reaction zones are shown, that is, display band 1 and colour developing band 2 are located at Region.In general, reaction zone can be set to predetermined size.Region in test paper other than reaction zone is non-reaction Area, for example, the test paper white space in Fig. 2.

Content of predetermined substance detection system can also include optical signal detecting device 20.Optical signal detecting device 20 is used for After labeling reaction in reaction carriers is reacted with determinand, the image of the reaction zone of reaction carriers is detected, and to being detected To the image of reaction zone pre-processed.About structurally and operationally being arrived below in conjunction with Fig. 3 for optical signal detecting device 20 Fig. 6 is described in detail.

Content of predetermined substance detection system can also include content determination unit 30.Content determination unit 30 and optical signal Detection device 20 is electrically connected, for receiving the figure by pretreated detected reaction zone from optical signal detecting device 20 Peak light value detection signal is extracted as data, and from the image data by pretreated reaction zone, then, is utilized The peak light value detection signal extracted determines the content of the predetermined substance in the determinand.For example, practical new at this In one example of type, the image data after Integral Processing can be by the image data of pretreated reaction zone. In this case, content determination unit 30 can use the image data after Integral Processing to draw optical signal detecting value Distribution curve, the optical signal detecting Distribution value curve is for indicating optical signal detecting value with the variation relation of position.Then, content Determination unit 30 can extract peak light value detection signal from optical signal detecting Distribution value curve.Then, content determines single Member 30 is determined and is mentioned using the corresponding relationship between optical signal detecting value (such as T value or T/C value) and content of predetermined substance The corresponding content of predetermined substance of peak light value detection signal of taking-up.The corresponding relationship for example can be optical signal detecting value with The variation relation curve between correspondence table or optical signal detecting value and content of predetermined substance between content of predetermined substance, or Functional relation between person's optical signal detecting value and content of predetermined substance.For example, content determination unit 30 can by tabling look-up or Person calculates (for example, Mathematical) to determine content of predetermined substance.In the present invention, the predetermined substance is to need to detect High molecular weight protein and small molecule compound, for example, the predetermined substance may include one of following: toxin, antibiotic, Pesticide or hormone.In another example of the utility model, light letter can be by the image data of pretreated reaction zone Number detected value distribution curve.Then, content determination unit 30 can extract peak light value detection signal as described above, and Using the corresponding relationship between optical signal detecting value and content of predetermined substance, the peak light value detection signal for determining and being extracted Corresponding content of predetermined substance.

It can be communicated by the way of wired or wireless between content determination unit 30 and optical signal detecting device 20. The example of the wire communication for example can be USB, serial ports, cable, coaxial cable, earphone jack etc..The wireless communication Example for example can be bluetooth, WiFi, microwave etc..

Content of predetermined substance detection system can also include host computer 40.Host computer 40 is electrically connected with content determination unit 30 It connects, for receiving identified content of predetermined substance from content determination unit 30, and content of predetermined substance inspection is presented to user Survey result.The example of host computer 40 for example can include but is not limited to mobile phone, PC, plate, single-chip microcontroller or server etc..In addition, Host computer 40 can also be electrically connected with optical signal detecting device 20, be determined for the operation to optical signal detecting device 20, content Operation (for example, interactive operation) between the operation of unit 30 and optical signal detecting device and content determination unit 30 carries out Control.

In the present invention, content determination unit 30 can be realized for example in server beyond the clouds, also may be embodied in It is realized in host computer 40.

Optical signal detecting device 20 can also include temperature sensor, be in detection shape in the reaction carriers for detecting Temperature data when state.In this application, detected temperature data can be used for selecting corresponding specific substance concentration with Optical signal detecting value change curve, that is, for characterizing pair between optical signal detecting value as described above and content of predetermined substance The curve that should be related to.

First embodiment

Fig. 3 shows the block diagram of the optical signal detecting device 20 of first embodiment according to the present utility model.

As shown in figure 3, optical signal detecting device 20 includes at least one light source 210.At least one light source 210 is arranged For the reaction zone for irradiating the reaction carriers after reacting with determinand.Preferably, at least one light source 210 be arranged to from it is to be measured It is irradiated above the reaction zone of reaction carriers after object reaction.In an example of the utility model, at least one light Source 210 can be arranged in the top of the reaction zone of the reaction carriers after reacting with determinand.In another example of the utility model In, optical signal detecting device 20 can also include optical path switching device (not shown).The optical path switching device is arranged at least one Between a light source 210 and the reaction zone of reaction carriers 10, the light for issuing at least one light source 20 carries out light path converting, So that being irradiated above the reaction zone of reaction carriers 10 from the light that at least one light source 20 issues.It is practical new at this In type, the optical path switching device can be made of one or more pieces plane mirrors or lens.

In the present invention, the effect of light source 210 is to issue the light of required wavelength, after being irradiated on test paper, benefit It is detected with optical signal detector 220.In an example of the utility model, light source 210 can be complete wavelength range or The light source of particular range of wavelengths, for example, LED light, wherein the higher green LED lamp of preferred signals response.

In the present invention, when reaction carriers (for example, fluorescent test paper) needs are detected according to fluorescence, light source 210 can be fluorescence excitation light source, and imaging sensor 220 has and the matched light-filtering characteristic of wavelength of fluorescence that is excited. For example, can by be arranged on the light source optical path of the ordinary light source light source of white light (that is, issue) optical filter of specific wavelength come The light source with respective wavelength is obtained, or the specific light source that can issue fluorescence can be used.Moreover it is preferred that for mistake Veiling glare is filtered, optical filter can also be set in the optical path of fluorescent light source.Furthermore, it is possible in the light path of imaging sensor 220 The optical filter that upper increase matches with the wavelength of fluorescence being excited, the preferably better narrow band filter of filter effect.

Optical signal detecting device 20 can also include imaging sensor 220.Imaging sensor 220 is arranged at least one light At the Image Acquisition feeder connection in source 210, for acquiring the image information of the reaction carriers 10 in reaction zone.In the utility model In, described image information may include colouring information and/or grayscale information.Wherein, described for colloidal gold or fluorescent test paper Image information can only include grayscale information.For certain drying chemical reagent papers, colouring information is needed.In the present invention, The effect of imaging sensor 220 is that the optical signal in reaction zone is converted to electric signal.The example of imaging sensor 220 can be with Including cmos sensor, ccd sensor etc..In the present invention, optical signal detecting device 20 can also have setting scheming As the camera lens between sensor 220 and reaction carriers 10.

Optical signal detecting device 20 can also include image pre-processor 230.Image pre-processor 230 and described image Sensor electrical connection, pre-processes for the image to reaction carriers 10 collected.Then, image pre-processor 230 will The content being arranged in host computer or cloud server is sent to by the image data of pretreated reaction carriers 10 to determine Unit 30, so that content determination unit 30 determines content of predetermined substance.

In an example of the utility model, image pre-processor 230 may include image interception module (not shown). Image interception module is electrically connected with imaging sensor 220, is used for according to pre-set image acquisition window location parameter, to collected The image of reaction carriers 10 carries out intercept operation.Here, pre-set image acquisition window location parameter refers to comprising test paper or reagent Reaction zone predeterminable area location parameter, that is, the size of pre-set image acquisition window is more than or equal to reaction carriers The size of reaction zone.In the present invention, pre-set image acquisition window location parameter can be based on the production of reaction carriers batch It is secondary to be arranged.

In addition, image pre-processor 230 can also include image compression module (not shown).Image compression module and image Interception module electrical connection carries out image Compression for the image data to the reaction carriers 10 intercepted.

In the present invention, above-mentioned image Compression can use addition process or Integral Processing to realize.? When being realized using Integral Processing, image Compression module may include that region division submodule, integral submodule and curve are drawn Molding block.Region division submodule is electrically connected with image interception module, and for being based on position, image information is divided into multiple rows Region.After such as uplink region division, integral submodule is used to carry out optical signal detecting value included in each row region Integral.Drawing of Curve module is used to draw light based on integral submodule each integrated value obtained and corresponding positional value Value detection signal distribution curve.The optical signal detecting Distribution value curve is used to indicate that optical signal detecting value to be closed with the variation of position System.In the present invention, test paper or reagent and reaction zone react be chromatography reaction when, optical signal detecting value is carried out Integral is carried out along the direction of chromatography reaction.Test paper or reagent and reaction zone react be that color lump uniformly reacts when, The integral carried out to optical signal detecting value can be carried out along any direction.In another example of the utility model, curve is drawn Molding block can be realized in host computer.

In the present invention, image pre-processor 230 and/or its component can use the device with computing capability It realizes, for example, DSP, FPGA or the digital circuit unit with computing capability come using processor, microprocessor, single-chip microcontroller It realizes.

In the present invention, optical signal detecting device 20 can also include buffer.Buffer is arranged in image sensing Between device 220 and image pre-processor 230, for caching the image information of reaction carriers 10 collected.In the utility model In, buffer can be realized using fifo chip, can also be realized using memories such as ROM, RAM.Collected anti- When the image information of carrier 10 being answered to store in both the buffers, the reading opportunity of the image information cached and reading speed can be by Image pre-processor 230 determines.

Optionally, optical signal detecting device 20 can also include communication unit 240.The example of communication unit 240 can wrap Include USB device, serial port device, network device, coaxial cable, earphone jack, blue-tooth device, RF device, Zigbee, infrared list Member, FM device, AM device or WIFI device etc..Communication unit 240 is arranged in image pre-processor 230 and content determination unit 30 Between, for realizing the data communication between image pre-processor 230 and content determination unit 30.

Using above-mentioned optical signal detecting device, the image information of the reaction zone of reaction carriers is obtained in imaging sensor, is led to It crosses according to pre-set image acquisition window location parameter and image interception operation is carried out to abandon original image to acquired image The invalid information for including in information, and image Compression is carried out to institute's truncated picture information, it is then sent to host computer It is determined to carry out content of predetermined substance.In the manner described above, the invalid information sent in the image information of host computer is removed, And Image Data Compression processing also is carried out to the image information after invalid information removes, is uploaded so as to greatly reduce To the data volume of the image information of host computer, the data transmission speed between optical signal detecting device and host computer is thus improved Rate reduces energy consumption.Further, since transmitted data amount greatly reduces, so that loading error occurring probability also correspondingly greatly reduces, simultaneously Since data have already passed through pretreatment, the performance requirement of host computer is greatly reduced.

In addition, by the way that buffer is arranged in optical signal detecting device 20, so that image pre-processor is not necessarily to pass image Sensor acquired image information is handled in real time, therefore without chip and algorithm that real-time Transmission image and data calculate, The performance and configuration requirement for thus greatly reducing image pre-processor 230, further reduced optical signal detecting device 20 Cost improves system working efficiency.In addition, the volume of optical signal detecting device 20 can be made very small, family is facilitated to detect.

Optionally, optical signal detecting device 20 can also include timer 2 50.Timer 2 50 and each optical signal detecting Device 220 is electrically connected, and for carrying out timing after reaction carriers 10 enter detection ready state, and light is triggered when timing expires Signal detector 220 carries out optical signal detecting.Optical signal detecting device 20 can also include parameter set unit 260.Parameter is set Unit 260 is set for the property according to the marker on predetermined substance to be measured and reaction carriers 10, the figure of imaging sensor is set As acquisition time started, Image Acquisition deadline and Image Acquisition gap periods.For example, parameter set unit 260 is arranged Image Acquisition time started, Image Acquisition deadline and the Image Acquisition gap periods of imaging sensor 220.Timer 250 are electrically connected with parameter set unit 260, to obtain set detection parameters from parameter set unit 260, for example, image Image Acquisition time started, Image Acquisition deadline and the Image Acquisition gap periods of sensor.

In the case where optical signal detecting device has timer, optical signal detecting device only reaches in timer specified When after just understand moment work acquisition image data, save real-time Transmission processing image section required for circuit, together When reduce performance requirement to processor, simultaneously because only moment work, significantly reduce power consumption and fever.

Optionally, in addition, optical signal detecting device 20 can also include the timing trigger unit being connected with timer 2 50 270.Timing trigger unit 270 is used for the triggering timer 2 50 after reaction carriers 10 enter detection ready state and starts timing.Example Such as, when reaction carriers 10 (for example, reagent strip) are put into optical signal detecting device 20, such as when insertion test paper, timing triggering is single Member 270 triggers timer 2 50 and starts timing.The way of realization of timing trigger unit 270 can include but is not limited to, mechanically Trigger unit (such as microswitch), photo-electric trigger unit (such as photoelectric sensor), magnetic-type trigger unit (such as Hall Element).Using timing trigger unit 270, adopted in combination with the image of imaging sensor set by parameter set unit 260 Collection triggered time and Image Acquisition gap periods, optical signal detecting device 20 can be adapted for the reagent of differential responses time, To realize the detection for being directed to different predetermined substances.

In an example of the utility model, for the irradiation control of light source 210 and the image of imaging sensor 220 Sensing can be taking human as being controlled.For example, can be by artificially opening the power switch of light source 210 and imaging sensor 220 Come so that light source 210 and imaging sensor 220 work.

In another example of the utility model, optical signal detecting device 20 can also include controller, for each The irradiation of light source and the detection of imaging sensor are controlled.In addition, the controller can also be to optical signal detecting device Data communication between the data communication between various components in 20 and/or the component in optical signal detecting device 20 and outside It is controlled.In the present invention, controller can use processor, microprocessor, single-chip microcontroller, DSP, FPGA or other Digital circuit unit with processing capacity is realized.

Second embodiment

Fig. 4 A shows the specific implementation example of the optical signal detecting device 400 of second embodiment according to the present utility model Outer shape schematic diagram.As shown in Figure 4 A, optical signal detecting device 400 includes shell 401, device mainboard 403 and structure branch Support member 405.Fig. 4 B shows the exemplary internal structure chart of specific implementation shown in Fig. 4 A.As shown in Figure 4 B, device mainboard 403 In be disposed with the various components of optical signal detecting device 400.In the present invention, device mainboard 403 can use printing electricity Road is realized.Device mainboard 403 can be fixed on structural support 405 by location hole and buckle.In the utility model In, reagent card accommodation space has can be set in structural support 405, for accommodating reagent card 407.Further optionally, structure branch Support member 405 is also provided with battery accommodating space 408, for accommodating battery (for example, lithium battery).For example, passing through bonding work Lithium battery is fixed in the respective slot of structural support 405 by skill.When needing to charge, by USB charge port to lithium battery It charges.

Fig. 4 C shows the side view of the arrangement of the component on device mainboard 403, and Fig. 4 D shows the component of device mainboard 403 The top view and Fig. 4 E of arrangement show the bottom view of the component arrangement of device mainboard 403.

At least one light source 411, imaging sensor 412, camera lens 413, temperature can be disposed on device mainboard 403 to pass Sensor 414, bluetooth module 415, cache module 416 and single-chip microcontroller 417.In one example, at least one light source 411, image Sensor 412, camera lens 413 and temperature sensor 414 can be arranged on the back side of device mainboard 403, as shown in fig.4d. Bluetooth module 415, cache module 416 and single-chip microcontroller 417 are arranged on the front of device mainboard 403, as shown in figure 4e.

In fig. 4d, at least one light source 411 includes two light sources, and the two sides of camera lens 413 are arranged in.In the utility model Other examples in, more than two light source can be used.Imaging sensor 412 is used to sense the reaction zone of reaction carriers 10 Image.Imaging sensor 412 can be realized using CMOS sensor, for example use gc0308CMOS sensor.Camera lens 413 act on the front of imaging sensor 412, acquire the letter of the reaction zone of reaction carriers for cooperating with imaging sensor Number.Temperature sensor 414 is used to detect the temperature data when reaction carriers are in detecting state, for selecting corresponding light Value detection signal-concentration relationship curve, the optical signal detecting value-concentration relationship curve can be T value and concentration relationship curve, It is also possible to T/C value and concentration relationship curve.

Bluetooth module 415 is for optical signal detecting device 400 and external data communication.Here, bluetooth module 415 can be with It is replaced using the communication unit of various other types.Cache module 416 is used for the figure that cache image sensor 412 is sensed Picture.

Single-chip microcontroller 417 pre-processes for realizing the image sensed to imaging sensor 412.For example, single-chip microcontroller 417 may be implemented the function of above-mentioned image pre-processor.In this example, single-chip microcontroller can be realized using stm32f103c8u6. In addition, single-chip microcontroller 417 can also realize the function of above controller.

Optical signal detecting device 400 can also include microswitch (not shown).Here, microswitch may be implemented above-mentioned The function of timer timing trigger unit.Optical signal detecting device 400 can also include USB charge port and power switch.

In addition, in the specific implementation example shown in Fig. 4 A-4E, at least one light source 411, imaging sensor 412, mirror First 413, temperature sensor 414, bluetooth module 415, cache module 416 and single-chip microcontroller 417 are arranged in same printed circuit board On.In the other examples of the utility model, above-mentioned module or unit may not necessarily be also arranged on same printed circuit board.

In addition, optical signal detecting device 400 can also be equipped with shading piece on structural support 405, for excluding veiling glare Interfere the influence to testing result.For example, between the members or all components in reagent card 407 and device mainboard 403 Shading piece is set.Specifically, such as with the corresponding position of light source and optical signal detector on device mainboard 403 it arranges Shading piece.

When executing detection, reagent card 407 can be inserted in the reagent card accommodation space of structural support 405, and quasi- Determine position.When detecting that reagent card is inserted in place (that is, reaction carriers are in detection ready state), microswitch is triggered, from And start timing using the timing function being arranged in single-chip microcontroller 417, thus complete the optical signal value in each period of chromatography Detection.

Single-chip microcontroller 417 can respond instruction and obtain related measurement data and report to host computer, also can control optical signal Various components in detection device 400 execute corresponding function and summarize and handle acquired data.

Above-mentioned apparatus can be used for the test paper with color change after detection is reacted with determinand, contain to detect determinand Amount.

Further, it is also possible to which the exemplary improvement of specific implementation shown in Fig. 4 A-4E is a difference in that and is changed to light source Fluorescence excitation light source, and imaging sensor is instead able to detect the imaging sensor for the fluorescence that is excited, for example, in ordinary light source The light source optical path setting of (that is, the light source for issuing white light) is directed to the optical filter of specific wavelength, so as to realize with corresponding wave Long first light source (for example, fluorescence excitation light source), and optical filter is set in the optical path of imaging sensor 220, carry out phase The optical signal value of wavelength is answered to detect (for example, the optical signal detecting for being directed to fluorescent test paper).For example, can be in the image of the shading piece The corresponding position of sensor 220, increases and optical filter that the fluorescent light source that is excited matches, optical filter are used to issue light source Light carry out wavelength filtering.And optical filter can be increased, below light source to filter veiling glare.Utilize above-mentioned optical signal detecting Device can be applied to the optical signal detecting of fluorescent test paper or fluorescent reagent card.The optical signal detecting being illustrated in figures 4A and 4 B In device, used camera lens model ANM12FH2814, correspondingly, the plant bulk of optical signal detecting device are 10cm* 10cm*1cm。

3rd embodiment

Fig. 5 A and Fig. 5 B show one of the optical signal detecting device of 3rd embodiment according to the present utility model specifically The structural schematic diagram of implementation example.

Specific configuration is as shown in Figure 5 B, and optical signal detecting device is made of the part C1, the part C2 and the part C3.Wherein, C1 Part is reaction carriers (reagent strip) socket portion, and the part C2 is device main board (that is, above-mentioned PCB main board) and the portion C3 Dividing is mainboard bracket part (that is, above structure supporting element).The part C2 is fixed on the part C3 and by the part C3 and the part C1 It is mutually fixed, so that the part C2 is located at the surface of reagent strip (that is, part C4 in figure).In the detection process, strip is inserted into Into the part C1.

In the optical signal detecting device shown in Fig. 5 A and Fig. 5 B, used camera lens model MV-150-2F26, The size of the camera lens is less than the size of camera lens employed in Fig. 5 A.Correspondingly, the plant bulk of optical signal detecting device is 11cm*3.4cm*3.4cm。

Fourth embodiment

Fig. 6 shows the side view of the test strips (that is, reaction carriers) of fourth embodiment according to the present utility model.Such as Described in Fig. 6, the test strips of the utility model include bottom plate 1 and successively sequence overlap joint pastes sample pad 2, sample on bottom plate 1 Bonding pad 4, example reaction pad 5 and water absorption pad 14.

Bottom plate 1 is for carrying and fixing sample pad 2, sample bonding pad 4, example reaction pad 5 and water absorption pad 14.Bottom plate 1 Width can be of same size with sample pad 2, sample bonding pad 4, example reaction pad 5 and water absorption pad 14, or bigger.At one In embodiment, the width of bottom plate 1 is of same size with sample bonding pad 4, example reaction pad 5 and water absorption pad 14.The length of bottom plate 1 Length after degree can sequentially be overlapped with sample pad 2, sample bonding pad 4, example reaction pad 5 and water absorption pad 14 is identical, Huo Zhegeng It is long.In one embodiment, the length with sample pad 2 of bottom plate 1, sample bonding pad 4, example reaction pad 5 and water absorption pad 14 are suitable Length after sequence overlap joint is identical.In one embodiment, the material of bottom plate 1 can be plastics.

Sample pad 2 overlaps in the structure of test strips with sample bonding pad 4 for receiving sample.Sample pad 2 and sample The overlapping mode of bonding pad 4 includes that sample pad 2 is overlapped on 4 top of sample bonding pad, and sample pad 2 is overlapped under sample bonding pad 4 Side and sample pad 2 and sample bonding pad 4 staggeredly overlap.The material of sample pad 2 can be by glass fibre element film, polyester cellulose film Or non-woven fabrics is made.It optionally further comprise point sample area 3 in sample pad 2, this point sample area 3 and this test strips are wrapped in test card The region exposed afterwards from well position is corresponding.

The sample that sample pad 2 is received includes body fluid class sample and animal derived sample.The body fluid class sample includes blood Liquid, blood plasma, serum and urine.In one embodiment, the body fluid class sample is urine.In one embodiment, institute Stating animal derived sample is animal tissue.The design of sample pad 2 is so that test strips can detect the range expansion of sample, the sample of detection Product are also possible to animal derived food either body fluid.When sample is humoral sample, sample can be detected directly, be not necessarily to Pretreatment.

Sample pad 2 needs to handle by solution twice.Processing for the first time is that 2 entirety of sample pad is infiltrated on bovine serum albumin It carries out staying overnight closing in white (BSA) solution or other protein blocking agent, then in 37 DEG C of dryings.Second of processing is by aforementioned place Manage obtained sample pad 2 be further infiltrated on sample pad treatment fluid (50mM PBS+0.1-1%Tween-20+2.5% sucrose+ 0.1%Proclin 300), then in 37 DEG C of dryings.This treatment process enables the sample pad of the utility model to ensure to be connect The sample chromatographic effect of receipts more evenly, the result stable reading after therefore ensuring that.

Realize that quantitative detection, sample pad 2 have certain size for cooperation instrument.In one embodiment, sample pad 2 A length of 15-25mm, width 2-6mm, with a thickness of 0.5-1.0mm.In one embodiment, a length of the 20 of sample pad 2 ± 1mm, width is 4 ± 1mm, with a thickness of 0.7 ± 0.1mm.

Sample bonding pad 4 is overlapped with sample pad 2 and example reaction pad 5 respectively.Sample bonding pad 4 is taken with example reaction pad 5 The mode of connecing includes that example reaction pad 5 is overlapped on 4 top of sample bonding pad, and example reaction pad 5 is overlapped on 4 lower section of sample bonding pad, It is staggeredly overlapped with example reaction pad 5 and sample bonding pad 4.

The top of sample bonding pad 4 is further coated with antibody layer (not shown go out).The antibody layer includes that marker is sewed The first antibody of the anti-quinolone closed, the secondary antibody of the tetracycline resistance of marker conjugation and marker conjugation anti-sulfanilamide (SN) the Three antibody.The first antibody of the anti-quinolone of marker conjugation is that quinolone is used to prepare as immunogene.Implement at one In mode, the first antibody is monoclonal antibody or polyclonal antibody.The secondary antibody of tetracycline resistance of marker conjugation is Tetracycline is used to prepare as immunogene.In one embodiment, the secondary antibody is monoclonal antibody or polyclonal Antibody.The third antibody of the anti-sulfanilamide (SN) of marker conjugation is that sulfanilamide (SN) is used to prepare as immunogene.In one embodiment, The third antibody is monoclonal antibody or polyclonal antibody.In one embodiment, the first antibody, secondary antibody and Third antibody is monoclonal antibody.The use of monoclonal antibody can further increase the sensitivity of testing result and special Property.

It in the present invention, can be colloidal gold or fluorescent microsphere for the marker with antibody conjugate.At one In embodiment, the marker is colloidal gold.In one embodiment, the marker is fluorescent microsphere.In a reality It applies in mode, the antibody layer includes the tetracycline resistance of the first antibody of the anti-quinolone of colloidal gold conjugation, colloidal gold conjugation The third antibody of the anti-sulfanilamide (SN) of secondary antibody and colloidal gold conjugation.In one embodiment, the antibody layer includes that fluorescence is micro- The first antibody of the anti-quinolone of ball conjugation, the secondary antibody of tetracycline resistance of fluorescent microsphere conjugation and resisting for fluorescent microsphere conjugation The third antibody of sulfanilamide (SN).Colloidal gold and fluorescent microsphere can use commercially available colloidal gold and fluorescent microsphere.Colloidal gold and fluorescence The conjugation methods of microballoon and antibody are known in the art.The adjustment of the particle size of colloidal gold and fluorescent microsphere is also in this field In the ability of technical staff.

In the present invention, it is the compound of basic structural unit that term " quinolone ", which refers to that one kind has quinolone, It is referred to as, i.e. carbostyril compound.Quinolone includes but is not limited to Ciprofloxacin, Norfloxacin, Enrofloxacin, Ofloxacin Deng.In one embodiment, quinolone is Ciprofloxacin.In one embodiment, quinolone is Norfloxacin.At one In embodiment, quinolone is Enrofloxacin.In one embodiment, quinolone is Ofloxacin.In the present invention, Term " quinolone " and " carbostyril compound " may be used interchangeably.

In the present invention, it is C that term " tetracycline ", which is removed including chemical formula,₂₂H₂₄N₂O₈Tetracycline (CAS registration number 60-54-8) outside, a kind of compound with tetracycline for basic structural unit, i.e. tetracycline compound are also covered.Tetracycline Including but not limited to tetracycline, terramycin, aureomycin, fortimicin etc..In one embodiment, tetracycline is chemical formula C₂₂H₂₄N₂O₈Tetracycline (CAS registration number 60-54-8).In one embodiment, tetracycline is terramycin.In a reality It applies in mode, tetracycline is aureomycin.In one embodiment, tetracycline is fortimicin.In the present invention, term " tetracycline " and " tetracycline compound " may be used interchangeably.

In the present invention, term " sulfanilamide (SN) " refers to a kind of system with the compound that sulfanilamide (SN) is basic structural unit Claim, i.e. sulfonamides compound.Sulfanilamide (SN) includes but is not limited to sulphadiazine, sulfamethazine etc..In one embodiment, Sulfanilamide (SN) is sulphadiazine.In one embodiment, sulfanilamide (SN) is sulfamethazine.In the present invention, term " sulphur Amine " and " sulfonamides compound " may be used interchangeably.

In one embodiment, the top of sample bonding pad 4 optionally includes the 4th antibody as Quality Control.At one In embodiment, the top of sample bonding pad 4 includes the 4th antibody as Quality Control.In one embodiment, it is used as Quality Control The 4th antibody can for marker conjugation goat-anti chicken IgY antibody.In one embodiment, the as Quality Control the 4th is anti- Body is polyclonal antibody.In one embodiment, the marker is colloidal gold.In one embodiment, the label Object is fluorescent microsphere.

In one embodiment, the top of sample bonding pad 4 does not include the 4th antibody as Quality Control, i.e. sample combines The top of pad 4 only include marker conjugation anti-quinolone first antibody, marker conjugation tetracycline resistance secondary antibody With the third antibody of the anti-sulfanilamide (SN) of marker conjugation.In one embodiment, the marker is colloidal gold.Implement at one In mode, the marker is fluorescent microsphere.

In one embodiment, by first antibody, secondary antibody, third antibody and the 4th antibody that is optionally present according to Certain ratio is mixed, with dilution (50-100mM Tris+5% trehalose+0.5%TritonX-100+1%BSA+ 300)+2% calf serum+0.5%Proclin of 1% sodium chloride carries out 20 times of dilutions, be spread evenly across sample bonding pad 4 Top is subsequently dried to get the sample bonding pad 4 of antibody layer is coated with.

In one embodiment, the quinolone of above-mentioned first antibody, secondary antibody and third antibody, Fourth Ring are used to prepare Element and sulfanilamide (SN) are respectively the conjugate of they and carrier protein.In one embodiment, carrier protein can be bovine serum albumin White, oralbumin, hemocyanin, thyroprotein or human serum albumins.

The material of sample bonding pad 4 can be by selected from glass fibre element film or polyester cellulose film or non-woven fabrics.

Realize that quantitative detection, sample pad 4 have certain size for cooperation instrument.In one embodiment, sample knot Close a length of 5-10mm, width 2-6mm, with a thickness of 0.5-1.0mm of pad 4.In one embodiment, the length of sample bonding pad 4 For 8 ± 1mm, width is 4 ± 1mm, with a thickness of 0.7 ± 0.1mm.

Example reaction pad 5 is overlapped with sample bonding pad 4 and water absorption pad 14 respectively.Water absorption pad 14 and example reaction pad 5 overlap Mode includes that example reaction pad 5 is overlapped on 14 top of water absorption pad, and example reaction pad 5 is overlapped on 14 lower section of water absorption pad and sample is anti- Should pad 5 and water absorption pad 14 staggeredly overlap.The material of example reaction pad 5 is nitrocellulose filter (NC film) or cellulose acetate film.

Three detection reaction zones are provided on example reaction pad 5, i.e. detection reaction zone 10,11 and 12 and a Quality Control Reaction zone, i.e. Quality Control reaction zone 13.In one embodiment, 3 detection reaction zones and 1 matter are provided on example reaction pad Control reaction zone.Detection reaction zone 10,11 and 12 can be respectively provided with quinolone, tetracycline and sulfanilamide (SN) as detection antigen.Detection is anti- The antigen sequence for answering area 10,11 and 12 to be included can change.In one embodiment, reaction zone 10,11 and 12 is detected The antigen for being included can arbitrarily be independently selected from quinolone, tetracycline and sulfanilamide (SN), and condition is that detection reaction zone 10,11 and 12 is wrapped The antigen contained is different.In one embodiment, detection reaction zone 10,11 and 12 separately includes quinolone, tetracycline and sulfanilamide (SN) As detection antigen.In one embodiment, detection reaction zone 10,11 and 12 separately includes quinolone, sulfanilamide (SN) and tetracycline As detection antigen.In one embodiment, detection reaction zone 10,11 and 12 separately includes tetracycline, quinolone and sulfanilamide (SN) As detection antigen.In one embodiment, detection reaction zone 10,11 and 12 separately includes tetracycline, sulfanilamide (SN) and quinolone As detection antigen.In one embodiment, detection reaction zone 10,11 and 12 separately includes sulfanilamide (SN), tetracycline and quinolone With as detection antigen.In one embodiment, detection reaction zone 10,11 and 12 separately includes sulfanilamide (SN), quinolone and Fourth Ring Element and as detection antigen.

Example reaction pad 5 can be further provided with four closing plot structures being spaced apart from each other, i.e. enclosed area 6-9.Enclosed area 6-9 is by the specific position to example reaction pad 5 using made by the closed method of fixed point.In one embodiment, 4 enclosed areas being spaced apart from each other can be further provided on example reaction pad 5.In one embodiment, in example reaction pad 5 Intermediate four positions being spaced apart from each other, the closing for being such as coated with or being infiltrated with 0.2%BSA solution respectively are subsequently dried, with Form four enclosed areas being spaced apart from each other.Depending on closing the amount of the BSA solution used, enclosed area 6-9 can be made only in sample The surface of reacting pad 5, or can also be formed in example reaction pad 5 surface and by permeate reached with surface location phase Corresponding lower part.In one embodiment, the spacing distance between the 6-9 of enclosed area is same or different.In a reality It applies in mode, the spacing distance between the 6-9 of enclosed area is 3 ± 1mm.Unique enclosed area is used in the utility model test strips Design, can be conducive to exclude the interference to result and false positive/negative findings, the sensitive of testing result can be greatly improved Degree and specificity.

The corresponding position in the top of enclosed area 6-9 is further coated with three detection reaction zones, i.e. detection reaction zone 10,11 With 12 and a Quality Control reaction zone, i.e. Quality Control reaction zone 13.It in one embodiment, can be further on example reaction pad 5 4 enclosed areas being spaced apart from each other are provided with, and 3 detection reaction zones and 1 Quality Control reaction zone are coated on each institute respectively State the top of enclosed area.

In one embodiment, as quinolone, the Fourth Ring for being used as detection antigen on detection reaction zone 10,11 and 12 Element and sulfanilamide (SN) may be the conjugate of they and carrier protein, wherein the carrier protein can be bovine serum albumin(BSA), egg white Albumin, hemocyanin, thyroprotein or human serum albumins.

When in use, sample to be tested is spread the test strips of the utility model from sample pad to sample bonding pad direction, and with Afterwards by be coated with marker conjugation anti-quinolone first antibody, marker conjugation tetracycline resistance secondary antibody and mark Remember the sample bonding pad of the third antibody of the anti-sulfanilamide (SN) of object conjugation.

If when one or more containing quinolone, tetracycline and sulfanilamide (SN) in sample to be tested, corresponding antibiotic will be with The antibody of corresponding marker conjugation combines on sample bonding pad, and then corresponding antigen site on this antibody is caused to be covered It covers.Therefore, when the antibody after this combination continues through example reaction pad, will be unable to again with the phase that is arranged on example reaction pad Answer antigen to combine, and then colloidal gold cannot assemble colour developing, thus corresponding detection line do not develop the color or develop the color it is shallower.Conversely, such as When not containing the one or more of quinolone, tetracycline and sulfanilamide (SN) in fruit sample to be tested, corresponding marker on sample bonding pad The antibody of conjugation can will then be combined with the corresponding antigens being arranged on example reaction pad, thus the colour developing of corresponding detection line compared with It is deep.

Quality Control reaction zone 13 can be antigen or antibody.In one embodiment, Quality Control reaction zone 13 is anti-for IgG It is former.In one embodiment, Quality Control reaction zone 13 is the antibody of anti-igg.In one embodiment, Quality Control reaction zone 13 is IgY antigen.In one embodiment, Quality Control reaction zone 13 is the antibody of anti-IgY.

When on sample bonding pad 4 there are when four antibody, the 4th antibody will with coated on Quality Control reaction zone 13 its is corresponding Antigen combine, with indicate detection be normally carried out.Therefore, in one embodiment, there are the 4th on sample bonding pad 4 Antibody, the 4th antibody is the goat-anti chicken IgY of marker conjugation, and Quality Control reaction zone 13 is chicken IgY.In one embodiment, There are the 4th antibody on sample bonding pad 4, the 4th antibody is the goat anti-rabbit igg of marker conjugation, and Quality Control reaction zone 13 is rabbit IgG。

When four antibody is not present on sample bonding pad 4, first antibody, secondary antibody and third antibody are directly and Quality Control Coated antibody carries out chromogenic reaction on reaction zone 13, to indicate being normally carried out for detection.Therefore, in one embodiment, On sample bonding pad 4 be not present the 4th antibody, and Quality Control reaction zone 13 be can be anti-with first antibody, secondary antibody and third antibody The antibody answered, such as sheep anti-mouse igg.

When example reaction pad 5 setting there are four be spaced apart from each other closing plot structure 6-9 when, detect reaction zone 10,11 and 12 And Quality Control reaction zone 13 is respectively positioned on corresponding enclosed area top, therefore between them spacing distance be also possible to it is identical or not With.In one embodiment, the spacing distance detected between reaction zone 10,11 and 12 and Quality Control reaction zone 13 is phase It is same or different.In one embodiment, the interval distance between reaction zone 10,11 and 12 and Quality Control reaction zone 13 is detected From being 3 ± 1mm.

The liquid outlet quantity for detecting reaction zone 10,11 and 12 can be 0.6-1.0 μ l/cm.In one embodiment, detection reaction The liquid outlet quantity in area 10,11 and 12 is independently selected from below one or more: 0.6 μ l/cm, 0.7 μ l/cm, 0.8 μ l/cm, 0.9 μ L/cm and 1.0 μ l/cm.The liquid outlet quantity of Quality Control reaction zone 13 can be 0.8-1.2 μ l/cm.In one embodiment, Quality Control is anti- Answer the liquid outlet quantity in area 13 to be independently selected from below one or more: 0.8 μ l/cm, 0.9 μ l/cm, 1.0 μ l/cm, 1.1 μ l/cm and 1.2μl/cm。

Water absorption pad 14 can be preferably made of velveteen by selected from being made of the material of velveteen, glass.It is fixed to realize for cooperation instrument Amount detection, water absorption pad 14 have certain size.In one embodiment, a length of 20-40mm of water absorption pad 14, width 2- 6mm, with a thickness of 0.5-1.0mm.In one embodiment, a length of 31 ± 1mm of water absorption pad 14, width are 4 ± 1mm, with a thickness of 0.7±0.1mm。

In order to which easy-to-use and cooperation instrument uses, the length of the test strips of the utility model in 6cm between 9cm, It is preferred that 7.5cm is between 8.5cm.The width of the test strips of the utility model in 2.5mm between 6mm, preferably 3.5mm extremely Between 4.5mm, most preferably 4 ± 0.1mm.The test strips of the utility model are providing sensitivity and are detecting the same of the range of linearity When, the dosage of antibody can be reduced by the length and/or width that reduce test strips, and production cost is greatly saved.

The test strips of the utility model can be used for detecting quinolone, tetracycline and the sulfanilamide (SN) being present in following sample residual It stays.In one embodiment, the quinolone, tetracycline and sulfanilamide (SN) are present in sample selected from the following: meat and its system Product, milk and its product, eggs and its product, humoral sample.In one embodiment, the meat and its products are pig Meat, beef, mutton, the flesh of fish, chicken or rabbit meat, preferably pork.In one embodiment, the milk and its product are ox Milk, goat milk or mare's milk, preferred milk.In one embodiment, the eggs and its product are egg, duck's egg, goose egg or quail Quail egg, preferably egg.In one embodiment, the humoral sample is blood, blood plasma, serum or urine, and preferred urine is excellent The urine chosen.

Fig. 7 shows the top view of the test card of fourth embodiment according to the present utility model.It is identical with Fig. 6 to Fig. 7 Appended drawing reference no longer carries out repeated description.

As shown in fig. 7, test card includes test strips, and the shell of test strips described in cladding, wherein the shell includes: It is provided with the card lid 15 of well 17 and detection hole 18, and and the pedestal 16 that is connected to each other of the card lid 15.

Well 17 is opened on the upside of sample pad 2, to expose the partial region of sample pad 2.The sample exposed The partial region of pad 2 corresponds to the point sample area 3 of Fig. 6.Detection hole 18 is opened on the upside of example reaction pad 3, to expose detection The whole region of reaction zone 10-12 and Quality Control reaction zone 13.Card lid 15 and pedestal 16 can be made of such as plastics.

In one embodiment, the length of well 17 is in 5mm between 15mm, and preferably 8mm is between 10mm.? In one embodiment, the width of well 17 is in 2.5mm between 6mm, and preferably 3.5mm is between 4.5mm.Implement at one In mode, the length of detection hole 18 is in 10mm between 25mm, and preferably 16mm is between 20mm.In one embodiment, it examines The width of gaging hole 18 is in 2.5mm between 6mm, and preferably 3.5mm is between 4.5mm.

Well 17 and detection hole 18 can be the shapes such as rectangle, square, circle, ellipse.In an embodiment party In formula, well 17 is ellipse, and detection hole 18 is rectangle.

The design of test card is so that test strips are more easily carried and saved.Moreover, test card may be implemented in corresponding inspection Survey the quantitative analysis in instrument to test strips testing result.

The test strips and test card of the utility model can detect quinolone, four by simultaneous quantitative in wide scope sample detection Ring element and sulfanilamide (SN), and there is superior sensitivity and specificity.The design that uses of cooperation instrument so that the utility model test strips It is low in cost with test card, it is easy to carry, it can greatly meet the need that people quickly detect Different field and batch samples It wants, therefore there is good industrialization value.

Example

Following instance should not be construed as being limited to example set forth herein for illustrating the utility model.

The preparation of 1. test card of example

The preparation of 1.1 colloid gold particles

500ml water for injection is measured, is added in the round-bottomed flask for being placed with stirrer, is added at one time a certain amount of 1% Gold chloride is stirred with heating mantle heats to boiling, is continued to heat 5min, is disposably rapidly added a certain amount of 1% citric acid three Sodium (gold chloride: trisodium citrate=1:1.5), the colloid gold particle that obtained size is about 40nm continue heating stirring to solution Color is no longer changed, and is stopped heating, is cooled to room temperature, and with water for injection fluid infusion to 500ml, it is standby to be placed in 4 DEG C of refrigerators preservations With.

The preparation of the antibody of 1.2 markers conjugation

Quinolone monoclonal antibody specific is that quinolone antigen is used to prepare as immunogene.Tetracycline specificity is single Clonal antibody is that tetracycline antigen is used to prepare as immunogene.Sulfamido monoclonal antibody specific is with sulfamido antigen It is prepared as immunogene.Goat-anti chicken IgY is that chicken IgY is used to prepare as immunogen immune goat.Used in the present embodiment Above-mentioned goat-anti chicken IgY purchased from the logical experimental material center difficult to understand of Luoyang one hundred, quinolone, tetracycline, sulfamido specific monoclonal are anti- Body is purchased from Biotechnology Co., Ltd, power side.

4 10mL centrifuge tubes are taken, it is each that 5ml colloidal gold solution is added, with 0.2M K₂CO₃Solution adjusts pH to 7.0-7.5, point It Jia Ru not 25-50 μ g quinolone monoclonal antibody specific, tetracycline monoclonal antibody specific, sulfamido specific monoclonal Antibody and goat-anti chicken IgY, stand 10-20min after mixing well；It is molten that it is separately added into suitable 10%BSA in the above solution Liquid continues to stand 10-20min after mixing to close the colloidal gold for being not bonded to antibody；10000rpm is centrifuged 10min, discards Clearly, respectively the 0.01M phosphate buffer of 500 μ L pH7.4 is added to be resuspended to get 4 species specificity Dan Ke in 4 centrifuge tubes Grand antibody-colloidal gold marker.

The preparation of 1.3 sample pads

Material using glass fibre element film as sample pad, and it is cut to size appropriate.Whole sample pad is infiltrated It carries out staying overnight closing in BSA solution, sample pad treatment fluid (50mM PBS+0.5%Tween-20+2.5% sugarcane is used after 37 DEG C of dryings 300) sugar+0.1%Proclin carries out secondary treatment, 37 DEG C of dryings.

The preparation of 1.4 sample bonding pads

Material using glass fibre element film as sample bonding pad, and it is cut to size appropriate.

4 kinds of colloidal gold labeled monoclonal antibodies prepared by embodiment 2 mix according to a certain percentage, with dilution (50- + 2% calf serum+0.5% of 100mM Tris+5% trehalose+0.5%TritonX-100+1%BSA+1% sodium chloride Proclin 300) 20 times of dilutions are carried out, it is uniformly laid on glass fibre element film, vacuum freezedrying is to get being coated with colloidal gold The sample bonding pad of labelled antibody.

The preparation of 1.5 example reaction pads

Material using nitrocellulose filter (NC film) as example reaction pad, and it is cut to size appropriate.

The BSA of the parallel spraying 0.2% of the spraying instrument region that four are spaced apart from each other on example reaction pad difference is marked using gold Solution, 37 DEG C of dryings, to form enclosed area.Again with gold mark spraying instrument in the position for corresponding to each enclosed area parallel spraying respectively 1.5-2.5mg/ml quinolone antigen, 1.5-2.5mg/ml tetracycline antigen, 0.5-1mg/ml sulfamido antigen and 0.5-1mg/ Quinolone antigen detection line Q, tetracycline antigen detection line T, sulfamido antigen detection line S and nature controlling line is made in ml chicken IgY respectively C, it is spare after 37 DEG C of dryings after spraying.

Quinolone detection line Q, tetracycline detection line are successively coated on example reaction pad from sample pad to water absorption pad direction T, sulfamido detection line S and nature controlling line C.Sulfamido antigen, tetracycline antigen and quinolone antigen are respectively sulfa antibiotics- Carrier protein, tetracycline antibiotics-carrier protein and quinolone antibiotics-carrier protein conjugate, wherein carrier egg White is bovine serum albumin(BSA).Nature controlling line C is chicken IgY antigen.

The assembling of 1.6 test cards

Successively sequence overlap joint pastes sample pad, sample bonding pad, example reaction pad and water absorption pad on bottom plate.It will assemble Plastic plate the test strips of one fixed width are cut into dedicated cutting machine, be subsequently filled into accordingly getting stuck and buckle well upper cover.It will be single The card and desiccant installed is packed into aluminium foil bag, room temperature preservation after sealing.

Application of 2. test card of example in detection urine antibiotic content

Urine sample is picked up with urine cup, with 4 drop of drop after dropper imbibition in test card well.By test paper card insertion after sample-adding Enter in optical signal detecting device shown in Fig. 4 A-4B in second embodiment and start timing, reads result when being incubated for 5min.Knot Fruit shows that there are sulfa antibiotics residuals in this urine sample.

Example 3: application of the test card in detection meat in antibiotic content

Take the meat of thumbnail size to mince, be added centrifuge tube in, add at mating extracting solution to centrifuge tube scale, sufficiently shake from Heart pipe about 1min, slightly stands, and with dropper Aspirate supernatant, 4 drop of drop is in test card well.By test card insertion the after sample-adding Start timing in optical signal detecting device shown in Fig. 4 A-4B in two embodiments, reads result when being incubated for 6min.As a result it shows Show that there are tetracyclines and sulfa antibiotics to remain in this meat sample product.

Example 4: application of the multi-joint reagent of antibiotic in detection eggs antibiotic content

One egg is squeezed into bowl, is sufficiently stirred evenly, draws egg liquid with dropper, 4 drop of drop is in equipped with mating extracting solution (50- 100mM phosphate buffer) centrifuge tube in, sufficiently shake centrifuge tube about 1min, dropper separately taken to draw liquid in centrifuge tube Body, 4 drop of drop is in multi-joint antibiotic detection reagent card well.Shown in Fig. 5 A-5B that reagent card is inserted into 3rd embodiment Optical signal detecting device in start timing, 8 minutes reading results.There is no antibiotic is residual in this eggs sample as the result is shown It stays.

Example 5: application of the multi-joint reagent of antibiotic in detection milk in antibiotic content

It takes a small amount of liquid milk into cup or other containers, is drawn with dropper, 4 drop of drop is in multi-joint antibiotic detection reagent card In well.It will start timing shown in Fig. 5 A-5B that reagent card is inserted into 3rd embodiment in optical signal detecting device, 7 points Clock reads result.There are quinolone antibiotics residuals in this milk sample as the result is shown.

5th embodiment

The detection of 5.1 instrument performances:

By using the colloidal gold of the measurement various concentration of optical signal detecting device shown in Fig. 4 A-4B in second embodiment Standard Quality Control card measures the stability of concentration, specific verification method to verify are as follows:

It the use of the concentration gradient prepared is 5mIU/ml, the definite value LH standard items of 25mIU/ml, 45mIU/ml are surveyed Examination, parallel 3 reagent cards of each concentration point, takes mean value to be counted.The one group of representative experimental data statistics being listed below:

According to the comparing result of above table, performance of the optical signal detecting device of the utility model in stability is slightly Better than existing scan-type instrument.And what the optical signal detecting device completion in this test in the utility model detected every time Average time is 3 seconds, but the average time of referencing schemes completion one-time detection is 30 seconds.

This is tested while being that optical signal detecting device supplies with the power supply unit that two identical carried charges are shown simultaneously Electricity is simultaneously measured, and experiment is charged to 100% when starting, after completing experiment, the immunoassay instrument remaining capacity of reference 80%, and the remaining capacity 99% of the optical signal detecting device of the utility model.

5.2 reagent card performance detections:

5.2.1 the rate of recovery and the coefficient of variation of reagent card

Respective 5 μ g/L of mark-on sulphadiazine, hydrochloric acid are distinguished in urine specimen, pork sample, egg sample, milk sample 5 μ g/L of terramycin, 5 μ g/L of Ciprofloxacin are carried out using optical signal detecting device shown in Fig. 4 A-4B in second embodiment Detection, calculates the respective rate of recovery and the coefficient of variation.Recovery of standard addition=(detected value-background values)/spiked levels * 100%, Background values is the item concentration value to be measured before the non-mark-on of original sample.

The recovery of standard addition of these types of sample mark-on illustrates that reagent card surveys these types of sample 90% or more as the result is shown The rate of recovery it is fine, reagent is functional.It is specifically shown in the following table 1.

Table 1

Ten cards are surveyed in parallel by the sample in table 1, calculate CV value, are obtained one group of degree of variation data, be see the table below 2.From number According to can be seen that CV value not more than 7%, degree of variation is smaller, and reagent performance is good.

Table 2

5.2.2 the sensitivity and specificity of test card are examined

5.2.2.1 sensitivity technique

Sulfa antibiotics, tetracycline antibiotics and quinolone antibiotics are used to the 0.01M phosphoric acid of pH7.4 respectively Salt buffer is diluted to various concentration, is detected with test card, and the antibiotic of various concentration is added drop-wise to the sample-adding of test card It is detected in optical signal detecting device shown in Kong Zhong, Fig. 4 A-4B being inserted into second embodiment, optical signal detecting device exists It is read after 5min.The result shows that this test card is to sulfa antibiotics, tetracycline antibiotics, quinolone antibiotics Sensitivity be respectively as follows: 1 μ g/L of sulphadiazine <, 1 μ g/L of sulfamonomethoxine < 1 μ g/L, dimethyl methyl amic metadiazine <； 1 μ g/L of aureomycin < 1 μ g/L, terramycin < 1 μ g/L, fortimicin < 1 μ g/L, tetracycline <；1 μ g/L of Ciprofloxacin <, oxygen Flucloxacillin < 1 μ g/L, oxolinic acid < 1 μ g/L, Norfloxacin < 1 μ g/L, Enrofloxacin < 1 μ g/L of 1 μ g/L, pefloxacin <, 1 μ g/L, Nuo Meisha star < of Enoxacin < 1 μ g/L, 1 μ g/L of flumequine <.

5.2.2.2 specific detection

By other common antibiotic medicine (Clenbuterol, chloromycetin, beta-lactam, macrolides, amino sugars Glycoside, salbutamol, Ractopamine) with the 0.01M phosphate buffer of pH7.4 500 μ g/L are diluted to, it is carried out with test card Read after detection, as a result quinolone, tetracycline, sulfamido three be not detected, illustrate that this test card does not occur with these drugs Cross reaction, specificity are good.

Optical signal detecting device according to the present utility model can be applied to the detection in various fields, such as food safety neck Domain, medical domain, environmental area etc., in field of food safety, optical signal detecting device according to the present utility model for example may be used For detecting toxin, antibiotic, the application of the equal sizes such as pesticide or hormone.

Specifically, above-mentioned optical signal detecting device can be used for detecting human body or animal body fluid (urine, blood, milk, saliva Liquid, tear) or food (meat, milk, egg, grain, tea, fruits and vegetables) or feed in toxin, antibiotic, pesticide or hormone-content.

In addition, optical signal detecting device according to the present utility model can be fabricated to micromodule equipment that can be portable, such as can To be made into the size 10*10*1cm of length, width and height hereinafter, weight 220g equipment below.

In addition, due to the portability and rapidity of optical signal detecting device, above-mentioned optical signal detecting device can be used for Family's detection, such as family quantifies or qualitative detection, and can be used for quickly detecting, such as fast quantification or qualitative detection.

One embodiment according to the present utility model provides a kind of optical signal detecting device, comprising: at least one light Source is arranged to the reaction zone of the reaction carriers after irradiation is reacted with determinand；Imaging sensor, be arranged in it is described at least one At the Image Acquisition feeder connection of light source, for acquiring the image information of the reaction carriers in the reaction zone；And figure It as preprocessor, is electrically connected, is carried out for the image information to the reaction carriers collected pre- with described image sensor Processing.

In an example of above-described embodiment, the optical signal detecting device can also include: buffer, be arranged in institute It states between imaging sensor and described image preprocessor, for caching the image of the reaction carriers collected.

In an example of above-described embodiment, the optical signal detecting device can also include: timer, with the figure As sensor electrical connection, for timing and when the timing expires, triggering described image sensor carries out Image Acquisition.

In an example of above-described embodiment, the optical signal detecting device can also include: parameter set unit, with Timer electrical connection, for the property according to the marker on predetermined substance to be measured and the reaction carriers, described in setting Image Acquisition time started, Image Acquisition deadline and the Image Acquisition gap periods of imaging sensor；And with it is described The timing trigger unit of timer electrical connection, for triggering the timer after the reaction carriers enter detection ready state Start timing.

In an example of above-described embodiment, described image preprocessor may include: image interception module, and described Imaging sensor electrical connection, is used for according to pre-set image acquisition window location parameter, to the figure of the reaction carriers collected As carrying out intercept operation；And image compression module, it is electrically connected with described image interception module, for described anti-to what is intercepted The image information of carrier is answered to carry out image Compression.

In an example of above-described embodiment, at least one described light source is arranged to anti-after reacting with determinand It answers and is irradiated above the reaction zone of carrier.

In an example of above-described embodiment, at least one described light source is arranged in the load of the reaction after reacting with determinand The top of the reaction zone of body.

In an example of above-described embodiment, the optical signal detecting device can also include: optical path switching device, cloth It sets between at least one described light source and the reaction zone of the reaction carriers, for carrying out light path converting at least one light source To be irradiated above the reaction zone of reaction carriers.

In an example of above-described embodiment, at least one described light source is fluorescence excitation light source and described image Sensor has and the matched light-filtering characteristic of wavelength of fluorescence that is excited.

In an example of above-described embodiment, the optical signal detecting device can also include: one or more optical filterings Piece is separately positioned between the light source and/or described image sensor and the reaction carriers and matches with specific wavelength.

In an example of above-described embodiment, the optical signal detecting device can also include: temperature sensor, be used for Detect the temperature data when the reaction carriers are in detecting state.

In an example of above-described embodiment, the optical signal detecting device can also include: communication unit, and setting exists It is pre- for described image between described image preprocessor and the content determination unit being arranged in host computer or cloud server Data communication between processor and the content determination unit.

In an example of above-described embodiment, the optical signal detecting device can also include: camera lens, be arranged described Between imaging sensor and the reaction zone of the reaction carriers.

In an example of above-described embodiment, the optical signal detecting device can also include: controller, with it is described extremely Other components electrical connection in few light source, described image sensor and/or a described device, for at least one described light The irradiation in source, the Image Acquisition of imaging sensor, data communication and/or described device between the various components in described device In component and outside between data communication controlled.

In an example of above-described embodiment, the various components in the optical signal detecting device are arranged in same printing On circuit board.

In an example of above-described embodiment, the optical signal detecting device can also include: structural support, be used for Support the various components in the optical signal detecting device；And shell.

In an example of above-described embodiment, the structural support is provided with for accommodating the anti-of the reaction carriers Answer carrier accommodation space；And/or the structural support is provided with the battery accommodating space for accommodating battery.

In an example of above-described embodiment, the optical signal detecting device can also include: to be arranged in the reaction Shading piece between carrier and members or all components in the optical signal detecting device.

Another embodiment according to the present utility model provides a kind of content of predetermined substance detection in determinand System, which is characterized in that the system comprises: reaction carriers；Optical signal detecting device as described above；And content determines list Member is electrically connected with the optical signal detecting device, for the image based on the pretreated reaction carriers, determines specific object The content of matter.

In an example of above-described embodiment, the system can also include: host computer, with the content determination unit It is electrically connected with the optical signal detecting device, for content of predetermined substance testing result to be presented to user and/or believes the light Behaviour between number operation of detection device, the operation of the content determination unit and described device and the content determination unit It is controlled.

In an example of above-described embodiment, server or host computer beyond the clouds is arranged in the content determination unit In.

In an example of above-described embodiment, the reaction carriers are reagent strips, the reagent strip include bottom plate and Successively sequence overlap joint pastes such as lower component: i) sample pad, ii on bottom plate) sample bonding pad, wherein the sample bonding pad Top is further coated with antibody layer, wherein ii-a) antibody layer include marker conjugation anti-quinolone it is first anti- The third antibody of the anti-sulfanilamide (SN) of the secondary antibody and marker conjugation for the tetracycline resistance that body, marker are conjugated, and be optionally present The 4th antibody as Quality Control, iii) example reaction pad, wherein be provided on the example reaction pad 3 detection reaction zones and 1 Quality Control reaction zone, wherein iii-a) it is described detection reaction zone be respectively be coated with quinolone first detection reaction zone, packet Had the second detection reaction zone of tetracycline, and be coated with the third detection reaction zone and iii-b of sulfanilamide (SN)) Quality Control reaction Area is fixed with antigen or antibody as Quality Control, iv) water absorption pad.

In an example of above-described embodiment, 4 envelopes being spaced apart from each other are further provided on the example reaction pad Closed zone, and 3 detection reaction zones and 1 Quality Control reaction zone are coated on the top of each enclosed area respectively.

In an example of above-described embodiment, a length of 15-25mm of the sample pad, width is 2-6 mm, with a thickness of 0.5-1.0mm。

In an example of above-described embodiment, a length of 20 ± 1mm of the sample pad, width is 4 ± 1 mm, with a thickness of 0.7±0.1mm。

In an example of above-described embodiment, a length of 5-10mm of the sample bonding pad, width 2-6mm, with a thickness of 0.5-1.0mm。

In an example of above-described embodiment, a length of 8 ± 1mm of the sample bonding pad, width is 4 ± 1 mm, thickness For 0.7 ± 0.1mm.

In an example of above-described embodiment, a length of 20-40mm of the water absorption pad, width is 2-6 mm, with a thickness of 0.5-1.0mm。

In an example of above-described embodiment, a length of 31 ± 1mm of the water absorption pad, width is 4 ± 1 mm, with a thickness of 0.7±0.1mm。

In an example of above-described embodiment, the spacing distance between 4 enclosed areas is same or different.

In an example of above-described embodiment, the spacing distance between 4 enclosed areas is 3 ± 1 mm.

In an example of above-described embodiment, the marker is colloidal gold or fluorescent microsphere.

In an example of above-described embodiment, the quinolone is selected from one or more of: Ciprofloxacin, promise fluorine are husky Star, Enrofloxacin and Ofloxacin；The tetracycline is selected from one or more of: tetracycline, terramycin, aureomycin and strength Mycin；And the sulfanilamide (SN) is selected from one or more of: sulphadiazine and sulfamethazine.

In an example of above-described embodiment, the antibody layer does not include the 4th antibody as Quality Control.

In an example of above-described embodiment, the quinolone, tetracycline and sulfanilamide (SN) are present in sample selected from the following In: meat and its products, milk and its product, eggs and its product, human body fluid sample.

In an example of above-described embodiment, the reaction carriers are test cards, and the test card includes: i) institute as above The test strips and ii stated) cladding test strips as described above shell, wherein the shell includes: ii-1) be provided with well With the Ka Gai of detection hole, wherein the well is opened on the upside of the sample pad, to expose the part of the sample pad Region, the detection hole are opened on the upside of the example reaction pad, anti-to expose the detection reaction zone and the Quality Control Answer the whole region and ii-2 in area) and the pedestal that is connected to each other of the Ka Gai.

The specific embodiment illustrated above in conjunction with attached drawing describes exemplary embodiment, it is not intended that may be implemented Or fall into all embodiments of the protection scope of claims." exemplary " meaning of the term used in entire this specification Taste " be used as example, example or illustration ", be not meant to than other embodiments " preferably " or " there is advantage ".For offer pair The purpose of the understanding of described technology, specific embodiment include detail.However, it is possible in these no details In the case of implement these technologies.In some instances, public in order to avoid the concept to described embodiment causes indigestion The construction and device known is shown in block diagram form.

The foregoing description of present disclosure is provided so that any those of ordinary skill in this field can be realized or make Use present disclosure.To those skilled in the art, the various modifications carried out to present disclosure are apparent , also, can also answer generic principles defined herein in the case where not departing from the protection scope of present disclosure For other modifications.Therefore, present disclosure is not limited to examples described herein and design, but disclosed herein with meeting Principle and novel features widest scope it is consistent.

Claims (45)

1. a kind of optical signal detecting device, which is characterized in that the optical signal detecting device includes:

At least one light source is arranged to the reaction zone of the reaction carriers after irradiation is reacted with determinand；

Imaging sensor is arranged at the Image Acquisition feeder connection of at least one light source, for acquiring the reaction zone The image information of the interior reaction carriers；And

Image pre-processor is electrically connected with described image sensor, for the image information to the reaction carriers collected It is pre-processed.

2. optical signal detecting device as described in claim 1, which is characterized in that the optical signal detecting device further include:

Buffer is arranged between described image sensor and described image preprocessor, collected described anti-for caching Answer the image of carrier.

3. optical signal detecting device as claimed in claim 1 or 2, which is characterized in that the optical signal detecting device further include:

Timer is electrically connected with described image sensor, and for timing and when the timing expires, triggering described image is passed Sensor carries out Image Acquisition.

4. optical signal detecting device as claimed in claim 3, which is characterized in that the optical signal detecting device further include:

Parameter set unit is electrically connected with the timer, for according to the mark on predetermined substance to be measured and the reaction carriers The property for remembering object, is arranged the Image Acquisition time started, Image Acquisition deadline and Image Acquisition of described image sensor Gap periods；And

The timing trigger unit being electrically connected with the timer, for being triggered after the reaction carriers enter detection ready state The timer starts timing.

5. optical signal detecting device as claimed in claim 3, which is characterized in that the optical signal detecting device further include:

Parameter set unit is electrically connected with the timer, for according to the mark on predetermined substance to be measured and the reaction carriers The property for remembering object, is arranged the Image Acquisition time started, Image Acquisition deadline and Image Acquisition of described image sensor Gap periods；And

The timing trigger unit being electrically connected with the timer, for being triggered after the reaction carriers enter detection ready state The timer starts timing.

6. optical signal detecting device as claimed in claim 1 or 2, which is characterized in that described image preprocessor includes:

Image interception module, is electrically connected with described image sensor, is used for according to pre-set image acquisition window location parameter, to institute The image of the reaction carriers of acquisition carries out intercept operation；And

Image compression module is electrically connected with described image interception module, for the image letter to the reaction carriers intercepted Breath carries out image Compression.

7. optical signal detecting device as claimed in claim 3, which is characterized in that described image preprocessor includes:

Image interception module, is electrically connected with described image sensor, is used for according to pre-set image acquisition window location parameter, to institute The image of the reaction carriers of acquisition carries out intercept operation；And

Image compression module is electrically connected with described image interception module, for the image letter to the reaction carriers intercepted Breath carries out image Compression.

8. optical signal detecting device as claimed in claim 4, which is characterized in that described image preprocessor includes:

Image interception module, is electrically connected with described image sensor, is used for according to pre-set image acquisition window location parameter, to institute The image of the reaction carriers of acquisition carries out intercept operation；And

Image compression module is electrically connected with described image interception module, for the image letter to the reaction carriers intercepted Breath carries out image Compression.

9. optical signal detecting device as described in claim 1, which is characterized in that at least one described light source be arranged to from It is irradiated above the reaction zone of reaction carriers after determinand reaction.

10. optical signal detecting device as claimed in claim 6, which is characterized in that described at least one light source arrangement be to The top of the reaction zone of reaction carriers after surveying object reaction.

11. optical signal detecting device as described in claim 1, which is characterized in that the optical signal detecting device further include:

Optical path switching device is arranged between at least one described light source and the reaction zone of the reaction carriers, for described At least one light source carries out light path converting to be irradiated above the reaction zone of the reaction carriers.

12. optical signal detecting device as described in claim 1, which is characterized in that at least one described light source is fluorescence excitation Light source and described image sensor have and the matched light-filtering characteristic of wavelength of fluorescence that is excited.

13. optical signal detecting device as described in claim 1, which is characterized in that the optical signal detecting device further include:

One or more optical filters are separately positioned between the light source and/or described image sensor and the reaction carriers And it is matched with specific wavelength.

14. optical signal detecting device as described in claim 1, which is characterized in that the optical signal detecting device further include:

Temperature sensor, for detecting the temperature data when the reaction carriers are in detecting state.

15. optical signal detecting device as described in claim 1, which is characterized in that the optical signal detecting device further include:

Communication unit, the content determination unit that described image preprocessor is set and is arranged in host computer or cloud server Between, for the data communication between described image preprocessor and the content determination unit.

16. optical signal detecting device as described in claim 1, which is characterized in that the optical signal detecting device further include:

Camera lens is arranged between described image sensor and the reaction zone of the reaction carriers.

17. optical signal detecting device as claimed in claim 1 or 2, which is characterized in that the optical signal detecting device is also wrapped It includes:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

18. optical signal detecting device as claimed in claim 3, which is characterized in that the optical signal detecting device further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

19. optical signal detecting device as claimed in claim 4, which is characterized in that the optical signal detecting device further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

20. optical signal detecting device as claimed in claim 5, which is characterized in that the optical signal detecting device further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

21. optical signal detecting device as claimed in claim 6, which is characterized in that the optical signal detecting device further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

22. optical signal detecting device as claimed in claim 7, which is characterized in that the optical signal detecting device further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

23. optical signal detecting device as claimed in claim 8, which is characterized in that the optical signal detecting device further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

24. the optical signal detecting device as described in any in claim 9 to 16, which is characterized in that the optical signal detecting dress It sets further include:

Controller is electrically connected with other components at least one described light source, described image sensor and/or described device, For between the various components in the Image Acquisition of irradiation, imaging sensor at least one light source, described device The data communication between component and outside in data communication and/or described device is controlled.

25. optical signal detecting device as claimed in claim 1 or 2, which is characterized in that each in the optical signal detecting device A component is arranged on same printed circuit board.

26. optical signal detecting device as claimed in claim 16, which is characterized in that the optical signal detecting device further include:

Structural support, the various components being used to support in the optical signal detecting device；

Shell, and/or

Shading piece between component some or all of is set in the reaction carriers and the various components.

27. optical signal detecting device as claimed in claim 26, which is characterized in that the structural support is provided with for holding Receive the reaction carriers accommodation spaces of the reaction carriers；And/or

The structural support is provided with the battery accommodating space for accommodating battery.

28. a kind of system for the content of predetermined substance detection in determinand, which is characterized in that the system comprises:

Reaction carriers；

Optical signal detecting device as described in any in claims 1 to 27；And

Content determination unit is electrically connected with the optical signal detecting device, for based on the pretreated reaction carriers Image determines the content of predetermined substance.

29. system as claimed in claim 28, which is characterized in that the system also includes:

Host computer is electrically connected with the content determination unit and the optical signal detecting device, for specific object to be presented to user Matter content detection result and/or to the operation of the optical signal detecting device, the operation of the content determination unit and described Operation between device and the content determination unit is controlled.

30. system as claimed in claim 29, which is characterized in that content determination unit setting beyond the clouds server or In host computer.

31. system as claimed in claim 28, which is characterized in that the reaction carriers are reagent strips, and the reagent strip includes Bottom plate and successively sequence the overlap joint stickup such as lower component on bottom plate:

I) sample pad,

Ii) sample bonding pad, wherein the top of the sample bonding pad is further coated with antibody layer, wherein

Ii-a) antibody layer includes the tetracycline resistance of the first antibody of the anti-quinolone of marker conjugation, marker conjugation The third antibody of the anti-sulfanilamide (SN) of secondary antibody and marker conjugation, and the 4th antibody as Quality Control being optionally present,

Iii) example reaction pad, wherein 3 detection reaction zones and 1 Quality Control reaction zone are provided on the example reaction pad, In,

Iii-a) the detection reaction zone is respectively the first detection reaction zone for being coated with quinolone, is coated with the second of tetracycline Reaction zone is detected, and is coated with the third detection reaction zone of sulfanilamide (SN), and

Iii-b) the Quality Control reaction zone is fixed with antigen or antibody as Quality Control,

Iv) water absorption pad.

32. system as claimed in claim 31, which is characterized in that be further provided with 4 on the example reaction pad mutually The enclosed area at interval, and 3 detection reaction zones and 1 Quality Control reaction zone are coated on each enclosed area respectively Top.

33. system as claimed in claim 31, which is characterized in that a length of 15-25mm of the sample pad, width 2-6mm are thick Degree is 0.5-1.0mm.

34. system as claimed in claim 33, which is characterized in that a length of 20 ± 1mm of the sample pad, width are 4 ± 1mm, With a thickness of 0.7 ± 0.1mm.

35. system as claimed in claim 31, which is characterized in that a length of 5-10mm of the sample bonding pad, width 2- 6mm, with a thickness of 0.5-1.0mm.

36. system as claimed in claim 35, which is characterized in that a length of 8 ± 1mm of the sample bonding pad, width be 4 ± 1mm, with a thickness of 0.7 ± 0.1mm.

37. system as claimed in claim 31, which is characterized in that a length of 20-40mm of the water absorption pad, width 2-6mm are thick Degree is 0.5-1.0mm.

38. system as claimed in claim 37, which is characterized in that a length of 31 ± 1mm of the water absorption pad, width are 4 ± 1mm, With a thickness of 0.7 ± 0.1mm.

39. system as claimed in claim 32, which is characterized in that the spacing distance between 4 enclosed areas is identical Or it is different.

40. system as claimed in claim 39, which is characterized in that the spacing distance between 4 enclosed areas is 3 ± 1mm。

41. the system as described in any one of claim 31-40, which is characterized in that the marker be colloidal gold or Fluorescent microsphere.

42. the system as described in any one of claim 31-40, which is characterized in that the quinolone is selected from following one kind It is or a variety of: Ciprofloxacin, Norfloxacin, Enrofloxacin and Ofloxacin；The tetracycline is selected from one or more of: Fourth Ring Element, terramycin, aureomycin and fortimicin；And the sulfanilamide (SN) is selected from one or more of: sulphadiazine and sulfanilamide (SN) dimethyl Pyrimidine.

43. the system as described in any one of claim 31-40, which is characterized in that the antibody layer does not include being used as matter 4th antibody of control.

44. the system as described in any one of claim 31-40, which is characterized in that the quinolone, tetracycline and sulfanilamide (SN) It is present in sample selected from the following: meat and its products, milk and its product, eggs and its product, human body fluid sample.

45. system as claimed in claim 28, which is characterized in that the reaction carriers are test cards, and the test card includes:

1) test strips, the test strips include bottom plate and successively sequence overlap joint pastes such as lower component on bottom plate:

I) sample pad,

Ii) sample bonding pad, wherein the top of the sample bonding pad is further coated with antibody layer, wherein

Ii-a) antibody layer includes the tetracycline resistance of the first antibody of the anti-quinolone of marker conjugation, marker conjugation The third antibody of the anti-sulfanilamide (SN) of secondary antibody and marker conjugation, and the 4th antibody as Quality Control being optionally present,

Iii) example reaction pad, wherein 3 detection reaction zones and 1 Quality Control reaction zone are provided on the example reaction pad, In,

Iii-a) the detection reaction zone is respectively the first detection reaction zone for being coated with quinolone, is coated with the second of tetracycline Reaction zone is detected, and is coated with the third detection reaction zone of sulfanilamide (SN), and

Iii-b) the Quality Control reaction zone is fixed with antigen or antibody as Quality Control,

Iv) water absorption pad, and

2) shell for coating the test strips, wherein the shell includes:

Ii-1 it) is provided with the Ka Gai of well and detection hole, wherein the well is opened on the upside of the sample pad, with sudden and violent Expose the partial region of the sample pad, the detection hole is opened on the upside of the example reaction pad, to expose the inspection The whole region of reaction zone and the Quality Control reaction zone is surveyed, and

) and the pedestal that is connected to each other of the Ka Gai ii-2.