CN208621626U - Brucella antibody quick and quantitative determination system - Google Patents
Brucella antibody quick and quantitative determination system Download PDFInfo
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- CN208621626U CN208621626U CN201721431777.9U CN201721431777U CN208621626U CN 208621626 U CN208621626 U CN 208621626U CN 201721431777 U CN201721431777 U CN 201721431777U CN 208621626 U CN208621626 U CN 208621626U
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Abstract
The utility model discloses a kind of brucella antibody quick and quantitative determination systems, including chromatography detection card, chromatogram scanner, client and cloud platform；The chromatogram scanner is scanned for carrying chromatography detection card, and to chromatography detection card；The chromatogram scanner is wirelessly connected client；Client connects cloud platform by internet.The cloud platform is for storing tested brucella antibody standard curve.Coating brucella lipopolysaccharides LPS monoclonal antibody is detection line on the nitrocellulose filter of the chromatography detection card, coating rabbit-anti chicken lgY antibody is nature controlling line, and marker is the brucella lipopolysaccharides LPS antigen and chicken lgY antibody nanosphere mixture of fluorescence detection microballoon coupling.The utility model can realize brucella antibody scene rapid quantitative detection, and for monitoring and evaluating immune effect of vaccine real-time, quickly, formulation science bridle strategy and evaluation animal population, which resist, to have great importance.
Description
Technical field
The utility model relates to a kind of detection systems, more particularly to a kind of brucella antibody rapid quantitative detection system
System.
Background technique
Brucellosis (Brucellosis, abbreviation cloth disease) is a kind of highly infective as caused by brucella
Zoonosis.Wherein sheep, ox, dog, pig etc. frequently as human world cloth disease main biography digital-to-analogue source, the mankind by location contact, disappear
Change the approach such as road, respiratory tract and obtain the disease, human health can be seriously endangered, and hinder animal husbandry development, influences the whole world and help through liquid
Trade.In China, cloth disease belongs to the legal Category B notifiable disease of law on the prevention and control of infectious diseases, is that the 2 class hearths of animal manage infectious disease surely, also
It is occupational disease.Sheep is the major source of infection of human world cloth disease.In sheep cloth disease epidemic-stricken area, crowd infection rate may be up to 42%, and illness rate is up to
7%.In recent years, which there is rebound significantly, and it is popular that " firing again " is worldwide presented.
Brucellosis is widely current in world many countries, and hotspot is Mediterranean Region, Asia, Central and South America
Deng.The annual new cases in the whole world about 500,000.This disease is popular serious before founding of New, has set up after founding of New special anti-
Mechanism is controlled, disease incidence has significantly reduced, but since nineteen ninety-four, and China's people and animals' brucellosis has rise again, much
Substantially the area controlled has new people and animals' brucellosis popular again, such as Binzhou Prefecture, Shandong Province, Hebei province Ci County and mountain
Xi Sheng, Liaoning Province etc..The main reason for generating Re-emergence is " without the free trade of quarantine domestic animal, exchange and flow ".This
Outside, loosen supervision, management, the disinfection to livestock products such as cream and meat, domestic animal cannot be immunized in time, extensively, and prevention and treatment troop melts
It dissipates, preventing and treating absent-mindedness paralysis etc. to brucellosis is all major reason.Therefore, we must reinforce to brucellosis
It prevents and treats to reach the target controlled substantially for a long time in China.
Since Brucella causes seriously broken ring to animal husbandry development and threatens the health of the mankind, Brucella is added
Belong to bacterial parasite intracellular, antibiotic treatment can not reach more satisfactory effect, the use of vaccine be to control most having for brucellosis
Efficacious prescriptions method, the same vaccine that detects generate the effective way that antibody is inspection immune effect of vaccine with prevents infection.
It is a large amount of using detection brucella enzyme-labeled antibody kits currently on the market, ELISA kit need microplate reader,
In addition incubation reaction condition, board-washing condition, operation element environment and professional technician detect sample and also need complicated preceding place
Reason such as acquires blood, separation serum etc.；And quickly colloidal gold is although easy to detect, is not required to professional technician and working environment,
But its sensitivity detected is low, and can only be qualitative, and detection range is narrow.Therefore, develop it is quick, convenient, can at the scene with field into
The Fast Detection Technique of row quantitative detection cloth disease antibody is particularly important.
Utility model content
The purpose of this utility model is just to provide a kind of brucella antibody quick and quantitative determination system, overcomes colloidal gold
The respective disadvantage of rapid detection card and enzyme Labeling Kit, by shirtsleeve operation, can at the scene with field to pig, ox, sheep and other
The progress of animal blood serum sample is quickly, accurate, quantitative detection, the technology had not only had the sensibility of ELISA with sensitivity, but also with glue
The convenience of body gold test paper strip, it may be said that fundamentally solve above-mentioned the deficiencies in the prior art place.
The purpose of this utility model is realized by following technical proposals:
A kind of quick and quantitative determination system of brucella antibody, including chromatography detection card, chromatogram scanner, client
And cloud platform；The chromatogram scanner is scanned for carrying chromatography detection card, and to chromatography detection card；The computed tomography scanning
Instrument is wirelessly connected client；Client connects cloud platform by internet, and the cloud platform is for storing brucella antibody mark
Directrix curve；The chromatography detection card includes the test strips for detecting card shell and being assemblied in detection card shell, the test strips packet
The plastic bottom board with pressure sensitive adhesive is included, sample pad, labeling pad, nitrocellulose filter and blotting paper, institute are successively pasted on bottom plate
It states labeling pad to be made of carrier substrate and marker, it is mono- that brucella lipopolysaccharides LPS is coated on the nitrocellulose filter
Resist for detection line, coating rabbit-anti chicken lgY antibody is nature controlling line, and marker is to be marked with brucella lipopolysaccharides LPS antigen
The fluorescence Quality Control mixture of microspheres of fluorescence detection microballoon and label chicken lgY antibody；The client is built-in with receiving module, at data
Module, memory module and communication module are managed, the receiving module, memory module and communication module connect with data processing module
It connects；Receiving module passes to data processing module for receiving the data that chromatogram scanner is transmitted to；Data processing module
It carries out data analysis and passes the result to memory module and communication module；Communication module connects cloud platform by internet.
Preferably, the brucella lipopolysaccharides LPS antigen is the LPS lipopolysaccharides of brucella purifying.
Preferably, the rabbit-anti chicken lgY antibody that nature controlling line uses on the nitrocellulose filter is the lgG of rabbit-anti chicken lgY.
Preferably, the client is with Internet enabled mobile phone or tablet computer.
Preferably, the lanthanide fluoro of the lanthanide fluoro microballoon of the label on biological raw material refer to lanthanide series Eu,
The fluorescence that Sm or Tb and beta-diketone ligand generate.
Preferably, the marker is that spraying lanthanide fluoro detects microballoon and lanthanide fluoro Quality Control microballoon in carrier substrate
The tunic formed.
Explanation of nouns:
The lgG of rabbit-anti chicken lgY: the immunoglobulin G of rabbit-anti chicken lgY；
Chicken lgY antibody: chicken yolk antibody.
Compared with prior art, the utility model has the beneficial effects that: brucella antibody scene can be realized quickly
Quantitative detection will provide efficient, sensitive, quick monitoring means and technology, practical valence with higher for China's prevention and control cloth disease
Value and promotional value.
Detailed description of the invention
Fig. 1 is the principles of the present invention block diagram；
Fig. 2 is the structural schematic diagram of chromatography detection card in the utility model；
Fig. 3 is the structural schematic diagram of test strips in Fig. 2；
Fig. 4 is the top view of Fig. 3；
Fig. 5 is brucella antibody test standard curve；
Fig. 6 is another structural schematic diagram of chromatography detection card.
Specific embodiment
The utility model is further described with attached drawing combined with specific embodiments below.
As shown in Figures 1 to 5, a kind of brucella antibody quick and quantitative determination system, including chromatography detection card, chromatography
Scanner, client and cloud platform.The chromatogram scanner is swept for carrying chromatography detection card, and to chromatography detection card
It retouches；The chromatogram scanner is wirelessly connected client；Client connects cloud platform by internet, and the cloud platform is for storing cloth
Lu Shi bacillus antibody standard curve.
The chromatography detection card includes the test strips being assemblied in shell 10, and the test strips include the plastics with pressure sensitive adhesive
Bottom plate 1 successively pastes sample pad 2, labeling pad 3, nitrocellulose filter 4 and blotting paper 5 from left to right on plastic bottom board 1,
And the right end of sample pad 2 rides on the left end of labeling pad 3, the right end of labeling pad 3 rides over the left end of nitrocellulose filter 4
On, 5 left end of blotting paper rides on the right end of nitrocellulose filter 4.It is more that brucella rouge is coated on the nitrocellulose filter 4
Sugared LPS monoclonal antibody is detection line 6, and coating rabbit-anti chicken lgY antibody is nature controlling line 7, and counter sample pad 2 opens up well on shell 10
11, the detection line 6 and nature controlling line 7 corresponded on nitrocellulose filter 4 opens up form hole 12.
As a kind of preferred structure of chromatography detection card, 10 surface of shell of the chromatography detection card corresponds to blotting paper 5
Position offers multiple through-holes 13, is conducive to solvent and volatilizes shell 10, it is ensured that testing result is accurate, solves the prior art
The technical issues of detection card shell uses enclosed construction, and solvent can not volatilize away, referring to Fig. 6.
In order to guarantee volatilization effect, equidistantly uniformly opened in the region that 10 surface of shell matches with 5 size of blotting paper
If through-hole 13.
As another preferred structure of chromatography detection card, the test strips inclination is fixed in shell 10, and sample pad 2
In low side, blotting paper 5 is in high-end.This structure makes the sample to be tested reduced velocity flow instilled from well 11, slowly past
Upper chromatography guarantees the enough chromatography time, and it is too fast and influence testing result to can effectively prevent liquid sample flow, avoids sample mistake
The fast nitrocellulose filter 4 that flows into causes testing result to fail.Because being easy to cause chromatography not if sample to be tested flow velocity is too fast
Sufficiently, and then testing result is influenced.The inclined angle of test strips is specifically designed according to the difference of sample to be tested.
As another preferred structure of chromatography detection card, it is equipped with holding tank in the shell 10 of the chromatography detection card, and
2 holding tanks are symmetrically arranged in the two sides of detection card ontology, sheet desiccant is installed in holding tank.This design is so that dry
Drying prescription keeps together with detection card ontology, so that detection card ontology humidity is effectively controlled, to improve detection accuracy.
As another preferred structure of chromatography detection card, 10 surface of the shell setting that can also detect card in chromatography is with cover
Sample reserve tank, sample can be sealed up for safekeeping.The hd top face of the sample reserve tank is flushed with 10 surface of shell, is covered equipped with recessed
Hole is uncapped convenient for users to finger force is turned-out.
The labeling pad 3 is made of carrier substrate and marker, and marker is spraying lanthanide fluoro inspection in carrier substrate
The tunic that micrometer ball and lanthanide fluoro Quality Control microballoon are formed, and marker is to be marked with brucella lipopolysaccharides LPS antigen
Fluorescence detection microballoon and label chicken lgY antibody fluorescence Quality Control microballoon.Lanthanide fluoro of the label on biological raw material is micro-
The lanthanide fluoro of ball refers to the fluorescence that lanthanide series Eu, Sm or Tb and beta-diketone ligand generate.
The brucella LPS boivin antigen is the lipopolysaccharides LPS antigen of brucella purifying.
The rabbit-anti chicken lgY antibody that nature controlling line uses on the nitrocellulose filter 4 is the lgG of rabbit-anti chicken lgY.
When carrying out chromatography detection, brucella antibody and brucella lipopolysaccharides LPS antibody are marked in blood sample
Object is immunoreacted to form compound, strives coating brucella LPS monoclonal antibody on nitrocellulose filter unexpectedly, is detected by striving method unexpectedly
Brucella antibody in pig, ox, sheep and other animal blood samples.
The client is built-in with receiving module, data processing module, memory module and communication module, the reception mould
Block, memory module and communication module are connect with data processing module；Receiving module is transmitted to for receiving chromatogram scanner
Data, and pass to data processing module；Data processing module carry out data analysis and pass the result to memory module and
Communication module；Communication module connects cloud platform by internet.The client is with Internet enabled mobile phone or plate electricity
Brain.
Communication module connects cloud platform by internet and interacts to realize with digerait, realizes cloud diagnosis, which examines
Disconnected to refer to that digerait provides diagnosis and guide service to client by internet, client supports data statistics, and according to net
Network expert needs that statistical data is sent to digerait by client, seeks technical support.
Working principle:
It after sample to be tested is diluted, instills in the well of chromatography detection card, chromatography detected chromatography after 15 minutes
Card is put into chromatogram scanner and is scanned, and the excitation light source in chromatogram scanner issues exciting light, shines chromatography detection card
It penetrates, so that the fluorescent microsphere on chromatography detection card is excited and generates fluorescence, chromatogram scanner is acquired point by point on chromatography detection card
Fluorescence signal intensity value, and then complete the detection of entire chromatography detection card.The chromatogram scanner will test data and pass through bluetooth
The receiving module of client is passed to, data processing module is calculated according to the fluorescence signal intensity detection data of chromatography detection card
Chromatography detection blocks the fluorescence signal intensity value of upper nature controlling line and detection line out, while data processing module passes through communication module from cloud
Platform obtains the standard curve of the brucella antibody of sample, and is stored in memory module, data processing module according to
Standard curve (contrast relationship of the fluorescence signal intensity value and standard curve of nature controlling line and detection line), is calculated sample
The content of middle brucella antibody, and shown, testing result is stored in memory module and cloud platform.
Prepare brucella antibody test standard curve:
6 parts of the calibration solution (antibody standard substance containing brucella) containing brucella antibody is configured, concentration is respectively
0,1/4,1/16,1/64,1/256,1/1024(brucella antibody standard substance doubling dilution).By the school of above-mentioned various concentration
Quasi- liquid is separately added into the well of the detection card assembled, and chromatography is detected, by 6 after 15 minutes by chromatogram scanner
For secondary obtained testing result by client process, client calculates the fluorescence signal of standard items corresponding detection line and nature controlling line
Intensity value, and carry out linear regression according to this data and make the standard curve of brucella antibody, client is by standard curve
It is transmitted to cloud platform storage, referring to fig. 4.
The standard curve that the client is calculated forms a file, and corresponding generation bar code, client pass through
Scanning bar code can extract the standard curve from cloud platform.
The application method of the detection card: pressing 1:50 dilution proportion for measuring samples (by taking serum as an example) and sample diluting liquid,
The sample 80ul diluted is added in well 11, under the action of blotting paper 5, sample is from sample pad 2 to 5 direction of blotting paper
It is mobile.15min is chromatographed in the case where avoiding strong illumination, then detection card is detected with chromatogram scanner, chromatography is swept
The fluorescence signal that instrument obtains detection line 6 and nature controlling line 7 is retouched, detection data is passed to client by bluetooth by chromatogram scanner, visitor
Family end calculates the fluorescence signal intensity value of detection line and the fluorescence signal intensity value of nature controlling line according to detection data, and client is logical
The bar code for over-scanning this batch of detection card obtains detected brucella antibody standard curve from cloud platform, and client is according to mark
It is anti-that brucella in prepare liquid is calculated in the contrast relationship of the fluorescence signal intensity value of directrix curve and nature controlling line and detection line
The potency of body, standard curve referring to fig. 4.Client can be mobile phone or tablet computer.
Above-mentioned detection card the preparation method is as follows:
Step 1 is pasted onto nitrocellulose filter on PVC bottom plate, using film metal spraying special purpose machinery in nitrocellulose
The lgG formation nature controlling line for being diluted to the goat-anti chicken lgY of 0.25mg/ml and the cloth Lu Shi for being diluted to 0.5mg/ml are sprayed on film
Bacillus LPS monoclonal antibody (purchasing hundred million minot Biotechnology Co., Ltd of Hangzhou) forms detection line, and discharge rate is 1 μ l/cm, then at 37 DEG C
At a temperature of bake 8 hours；
Step 2, preparation are marked with the lanthanide fluoro microballoon of chicken lgY and are marked with brucella lipopolysaccharides LPS antigen
The lanthanide fluoro microballoon of (purchasing hundred million minot Biotechnology Co., Ltd of Hangzhou), is added to 50mg for the lanthanide fluoro microballoon of 1mL
MES(2-(N- morpholine) ethanesulfonic acid) in buffer (0.1M, pH7.0), add 10mg carbodiimide (EDC) and 10mg
N-hydroxysuccinimide sulfonate sodium stirring and dissolving, room temperature reaction carry out centrifugally operated after 30 minutes, centrifugal sediment are used
50mM borate buffer (pH8.2) redissolves, and the chicken lgY that dialysed of 2mg is added, and is stirred to react 24 hours at room temperature, so
It is centrifuged afterwards, after closing, then preservation (Conservation environment temperature is 2~8 DEG C) is glimmering to get the group of the lanthanides for being marked with chicken lgY in dilution
Light microballoon；Using the lanthanide fluoro microballoon of above-mentioned same method label brucella lipopolysaccharides LPS antigen；
Step 3, the lanthanide fluoro microballoon for being marked with chicken lgY He being marked with brucella lipopolysaccharides LPS recombinant antigen
And it is diluted to concentration 0.1 μ g/ml and 3 μ g/ml respectively, it is sprayed in carrier substrate using a film metal spraying machine and constitutes label
Pad, discharge rate be 2.5 μ l/cm, then 37 DEG C at a temperature of bake 8 hours；
Step 4 is successively sticked to sample pad, label pad, blotting paper on PVC bottom plate, is assembled into kilocalorie, then use cutter
The test strips of 5mm wide are cut into, are assemblied in detection card shell to get this brucella antibody test card is arrived.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Made any modifications, equivalent replacements, and improvements etc., should be included in the utility model within the spirit and principle of utility model
Protection scope within.
Claims (6)
1. a kind of brucella antibody quick and quantitative determination system, it is characterised in that: including chromatography detection card, computed tomography scanning
Instrument, client and cloud platform；
The chromatogram scanner is scanned for carrying chromatography detection card, and to chromatography detection card；
The chromatogram scanner is wirelessly connected client；
Client connects cloud platform by internet, and the cloud platform is for storing tested brucella antibody standard curve and inspection
Measured data；
The chromatography detection card includes the test strips for detecting card shell and being assemblied in detection card shell, and the test strips include band
The plastic bottom board of pressure sensitive adhesive, assembling sequence, which is followed successively by bottom plate, pastes sample pad, labeling pad, nitrocellulose filter and water suction
Paper, the labeling pad are made of carrier substrate and marker, and brucella lipopolysaccharides is coated on the nitrocellulose filter
LPS monoclonal antibody is detection line, and coating rabbit-anti chicken lgY antibody is nature controlling line, and marker is the brucella rouge of fluorescent microsphere coupling
The nano-mixture of polysaccharide LPS antigen and chicken lgY antibody；
The client is built-in with receiving module, data processing module, memory module and communication module, the receiving module, storage
Module and communication module are connect with data processing module；Receiving module is used to receive the data that chromatogram scanner is transmitted to,
And pass to data processing module；Data processing module carries out data analysis and passes the result to memory module and communication mould
Block；Communication module connects cloud platform by internet.
2. brucella antibody quick and quantitative determination system according to claim 1, it is characterised in that: the cloth Lu Shi
Bacillus lipopolysaccharides LPS antigen is the LPS lipopolysaccharides of brucella purifying.
3. brucella antibody quick and quantitative determination system according to claim 1, it is characterised in that: the nitric acid is fine
The rabbit-anti chicken lgY antibody that nature controlling line uses on the plain film of dimension is the lgG of rabbit-anti chicken lgY.
4. brucella antibody quick and quantitative determination system according to claim 1, it is characterised in that: the client
For with Internet enabled mobile phone or tablet computer.
5. brucella antibody quick and quantitative determination system according to claim 1, it is characterised in that: the label exists
It is glimmering that the lanthanide fluoro of lanthanide fluoro microballoon on biological raw material refers to that lanthanide series Eu, Sm or Tb are generated with beta-diketone ligand
Light.
6. brucella antibody quick and quantitative determination system according to claim 1, it is characterised in that: the marker
The tunic that microballoon and lanthanide fluoro Quality Control microballoon are formed is detected to spray lanthanide fluoro in carrier substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721431777.9U CN208621626U (en) | 2017-11-01 | 2017-11-01 | Brucella antibody quick and quantitative determination system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721431777.9U CN208621626U (en) | 2017-11-01 | 2017-11-01 | Brucella antibody quick and quantitative determination system |
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| CN208621626U true CN208621626U (en) | 2019-03-19 |
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ID=65688398
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| CN201721431777.9U Active CN208621626U (en) | 2017-11-01 | 2017-11-01 | Brucella antibody quick and quantitative determination system |
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| Country | Link |
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| CN (1) | CN208621626U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111190004A (en) * | 2020-01-10 | 2020-05-22 | 上海泰辉生物科技有限公司 | Instant detection system for immunochromatography test strip |
| CN115541887A (en) * | 2022-08-02 | 2022-12-30 | 中国农业科学院北京畜牧兽医研究所 | Method for detecting content of specific IgG and IgM of brucellosis of cattle and application |
-
2017
- 2017-11-01 CN CN201721431777.9U patent/CN208621626U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111190004A (en) * | 2020-01-10 | 2020-05-22 | 上海泰辉生物科技有限公司 | Instant detection system for immunochromatography test strip |
| CN111190004B (en) * | 2020-01-10 | 2023-11-10 | 上海泰辉生物科技有限公司 | Instant detection system of immunochromatography test strip |
| CN115541887A (en) * | 2022-08-02 | 2022-12-30 | 中国农业科学院北京畜牧兽医研究所 | Method for detecting content of specific IgG and IgM of brucellosis of cattle and application |
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Effective date of registration: 20190404 Address after: 610000 North Section of Tianfu Avenue, Chengdu High-tech Zone, Sichuan Province, No. 1480, No. 1 Building, No. 2 and No. 3 Co-patentee after: China Veterinery Drug Inspection Office Patentee after: CHENGDU WEIRUI BIOTECHNOLOGY CO., LTD. Address before: 610000 North Section of Tianfu Avenue, Chengdu High-tech Zone, Sichuan Province, No. 1480, No. 1 Building, No. 2 and No. 3 Patentee before: CHENGDU WEIRUI BIOTECHNOLOGY CO., LTD. |
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