CN206270251U - Test strips data detection device - Google Patents
Test strips data detection device Download PDFInfo
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- CN206270251U CN206270251U CN201621036583.4U CN201621036583U CN206270251U CN 206270251 U CN206270251 U CN 206270251U CN 201621036583 U CN201621036583 U CN 201621036583U CN 206270251 U CN206270251 U CN 206270251U
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- test strips
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Abstract
The utility model provides a kind of test strips data detection device, it is characterised in that the detection means includes consisting of part：Signal collecting element, data processing equipment, and temperature acquisition element, wherein the data processing equipment further includes following component：Input element, the memory cell being connected with the input element, the analytic unit being connected with the memory cell, and the output element being connected with the analytic unit, and wherein described signal collecting element and the temperature acquisition element respectively connect the input element in the data processing equipment.Test strips data detection device of the present utility model has detection accuracy very high and sensitivity.
Description
Technical field
The utility model is related to clinical immunization detection means field, and in particular to it is a kind of based on temperature correction for detecting
The device of test paper data.
Background technology
Test paper has been obtained for being widely applied in the pattern detection of numerous areas, such as the urine commonly used in clinical treatment
Content detection of formaldehyde, benzene in conventional detection, environment measuring etc., is required for being detected using corresponding special test paper.
Some simple test paper inspections can observe by the naked eye test paper to judge, but this side by estimating test paper
Formula is typically only capable to qualitatively be detected, and when developing the color shallower, easily occurs misreading.Need to carry out quantitative determination
Test paper is typically in professional institution to be completed by distinctive equipment, and also some detections need the knot of comprehensive repeatedly inspection
Really, the micro-judgment for relying on professional could be completed.But, even the utility model designer notices professional institution
When carrying out qualitative/quantitative to the testing result of test paper and judging, influence of the temperature to testing result is also seldom considered.The utility model
When designer has found that same detection means works at different temperatures, the assay that it is given can have differences, i.e. temperature
The accuracy of testing result can be influenceed.
Accordingly, it would be desirable to one kind can realize it is free of extraneous thermal effects, using the teaching of the invention it is possible to provide the test paper data of objective and accurate result
Detection means.
Utility model content
Technical problem
At present, shortage one kind can be realized free of extraneous thermal effects, using the teaching of the invention it is possible to provide the test strips number of objective and accurate result
According to detection means.Therefore, the utility model provides a kind of device for Test paper data.
Technical scheme
On the one hand, the utility model provides a kind of test strips data detection device, it is characterised in that the detection means
Including consisting of part：
I) signal collecting element,
Ii) data processing equipment, wherein the data processing equipment further includes following component：
Ii-1) input element,
Ii-2) the memory cell being connected with the input element,
Ii-3) the analytic unit being connected with the memory cell, and
Ii-4) the output element being connected with the analytic unit,
Iii) temperature acquisition element,
Wherein, during the signal collecting element and the temperature acquisition element respectively connect the data processing equipment
The input element.
In one embodiment, the input element, memory cell, analytic unit, output element and temperature acquisition unit
One or more in part are positioned at the inside of the detection means.
In one embodiment, the input element, memory cell, analytic unit, output element and temperature acquisition unit
Part is respectively positioned on the inside of the detection means.
In one embodiment, the input element, memory cell, analytic unit, output element and temperature acquisition unit
One or more in part are positioned at the outside of the detection means.
In one embodiment, the input element, memory cell, analytic unit, output element and temperature acquisition unit
Part is respectively positioned on the outside of the detection means.
In one embodiment, the signal collecting element includes light signal collection element, magnetic signal acquisition element, electricity
Signal collecting element or picture signal acquisition elements.
In one embodiment, the signal collecting element includes camera.
In one embodiment, the data of the signal collecting element collection from test strips to be detected, wherein described
Test strips include cog region, control zone and test section.
In one embodiment, the temperature acquisition element is temperature sensor.
In one embodiment, the memory cell is one or more in hard disk, storage card and RAM card.
Beneficial effect
Test strips data detection device of the present utility model has the function based on temperature correction, is repaiied by way of calibration
Positive influence of the different temperatures to testing result, using the standard curve of different temperatures in memory cell, or to preset standard curve
Amendment, or according to temperature value and concentration of specimens value, the standard curve of the concentration value being fitted by computing ensures testing result
Accuracy, be conducive to clinical practice.
Brief description of the drawings
Fig. 1：One schematic diagram of the utility model test strips data detection device.
Fig. 2：Another schematic diagram of the utility model test strips data detection device.
Fig. 3：The schematic diagram of the test strips that the utility model device is detected.
Fig. 4：The response curve of hFABP under condition of different temperatures.
Fig. 5：HFABP is in 10 DEG C of standard curves of fitting.
Fig. 6：Standard curves of the cTnI at 21 DEG C.
Fig. 7：CKMB is in 25 DEG C of matched curves.
Fig. 8：The temperature of CKMB and temperature coefficient graph of a relation.
Specific embodiment
It is more fully described illustrative embodiments hereinafter with reference to the accompanying drawings.The accompanying drawing is used to illustrate this reality
With new, rather than it is limited.
Fig. 1 is a schematic diagram of the utility model test strips data detection device.
As shown in figure 1, test strips data detection device 1 of the present utility model includes signal collecting element 2, temperature acquisition unit
Part 3, and the data processing equipment including input element 4, memory cell 5, analytic unit 6 and output element 7.
Signal collecting element 2 is used to gather the signal from test paper, such as optical signal, magnetic signal, electric signal or image letter
Number etc..Signal collecting element 2 can include camera, its collection test paper image, and the edge in analysis identification colour developing area, interception meets
It is required that color development area, collection reference area (C lines) and test section (T lines) (see Fig. 3) color signal, be sent to input element 4.
Signal collecting element 2 can gather optical signal for example, by by photocell, avalanche diode or photomultiplier,
Or by CMOS, CCD collection picture signals, or magnetic signal is gathered by magnetic flux detection head, or electricity is gathered by electrode
Signal.
In one embodiment, before camera collection test paper image, camera gathers the identification code 201 of test paper first
(see Fig. 3), input element 4 is sent to by identification code 201, and input element 4 calls detection paper project, batch etc. from database
Information temporary storage simultaneously returns to mobile terminal.In one embodiment, before camera collection test paper image, user is pointed out in signal
Acquisition elements 2 select classification, the batch information of input test paper by hand, are sent to input element 4.In one embodiment, take the photograph
The image of whole test strips is gathered as head；Color and edge analysis are recognized, by presetting getting stuck and color development area for test paper
Aberration, find frame set in advance, select the region；Find out in this region and meet the region for presetting proportion requirement
As color development area, and intercept out.In one embodiment, camera gathers the image of whole test strips；Screening positioning
The position of point, by the position and level, the position of vertical direction that position point location color development area；Find out in this region and meet
The region of proportion requirement is preset as color development area, and is intercepted out.In one embodiment, do not have for third party
The common test paper of default frame, there is the view-finder for meeting test paper size on a display screen, points out user to be entangled entirely with view-finder
Test paper or color development area, and click on button of taking pictures.In one embodiment, 2 pairs of colour developing areas of interception of signal collecting element
Picture is standardized, and by photo finishing into unified pixel size, or will take pictures on time space or be offset in plane
Picture rotation return.
Temperature acquisition element 3 is used for the environment temperature that will be detected or conversion zone temperature is sent to input element 4.One
In individual implementation method, temperature acquisition element 3 can be such as temperature sensor.
Input element 4 is that the signal that will be collected is converted into data signal, that is, be converted into signal value.Obtained in input element 4
The signal for obtaining includes the signal from test paper test section and control zone, environment temperature, the information containing batch number etc..For example,
In input element 4, if the color value or gray value of reference area (C lines) are β, the color value or gray value of test section (T lines) are
α, by the related operation of α and β, such as operation values γ obtained by the ratio of T/C, α/β or beta/alpha or other computing modes.It is right
In nonimmune chromatographic test paper, by its Standard Colors blank pad or take edge, get stuck, the image of external packing, calculate color
Value or gray value, as reference area color value or gray value β.
Memory cell 5 is used to store the temperature of test paper or/and the data of calibration curve.Memory cell 5 stores different temperatures
The standard curve of lower testing sample.In one embodiment, in memory cell 5 comprising under different temperatures to preset standard curve
Correction factor and testing sample standard curve.In one embodiment, comprising according to temperature value and sample in memory cell 5
This concentration value, the standard curve of the concentration value being fitted by computing.In one embodiment, comprising difference in memory cell 5
At a temperature of testing sample standard curve, abscissa is concentration, and ordinate is signal value, and standard curve is directed to different lot numbers
Sample has each different standard curves.
Analytic unit 6 is used to, by transferring the information of input element 4 and the data of memory cell 5, carry out the computing of correlation
And analytic set.In one embodiment, the analytic set includes：Input temperature values, transfer and are best suitable for the temperature
Standard curve is modified according to the temperature to preset standard curve；And then establishing criteria curve calculates the concentration value of sample.
In one embodiment, the temperature correction can also refer to the sample content value γ of input temperature values and α/β, by computing
Concentration value after output calibration.
Output element 7 is used for the data storage after calibration and exports.
Fig. 2 is another schematic diagram of the utility model test strips data detection device.With Fig. 1 identicals part in Fig. 2
Repeat no more.
As shown in Fig. 2 the temperature acquisition element 3 in the utility model device, and including input element 4, memory cell
5th, analytic unit 6 and output element 7 can be entirely located in the outside of the detection means 1, they for example, by bluetooth, wifi or
The modes such as person internet realize the connection with signal collecting element 2.For example, can be single by input element 4, memory cell 5, analysis
Unit 6 and output element 7 are provided entirely in cloud server, and user can set up the health account database of oneself, are easy to knot
Fruit carries out long-term dynamics observation.CN201510397181.0 is can be found on above-mentioned high in the clouds technology.
In one embodiment, or many in input element 4, memory cell 5, analytic unit 6 and output element 7
The individual inside positioned at the detection means 1.In one embodiment, input element 4, memory cell 5, analytic unit 6 and defeated
Go out the inside that element 7 is respectively positioned on the detection means 1.In one embodiment, input element 4, memory cell 5, analysis are single
One or more in unit 6 and output element 7 are positioned at the outside of the detection means 1.In one embodiment, input element
4th, memory cell 5, analytic unit 6 and output element 7 are respectively positioned on the outside of the detection means 1.
The collection of signal collecting element 2 of detection means of the present utility model 1 includes control zone C lines, and test section T lines are gathered
Signal, the information of the grade of identification code 201 of test paper transmits these information to input element 4, while temperature acquisition element 3 will
The environment temperature or conversion zone temperature for being detected are sent to input element 4, and the signal of input is converted into signal by input element 4
Value.The standard curve of testing sample under the storage different temperatures of memory cell 5, or to preset standard curve under different temperatures
The standard curve of correction factor and testing sample, can also be according to temperature value and concentration of specimens value, by the dense of computing fitting
The standard curve of angle value.Analytic unit 6 is analyzed computing during the signal value of input element 4 is substituted into memory cell 5, obtains school
Concentration value after standard, is exported by output element 7.
Fig. 3 is the schematic diagram of the test strips that the utility model device is detected.
As shown in figure 3, test strips to be detected include identification code 201, color development area 202, and sample application zone S.Colour developing area
Domain 202 can further include control zone and test section.Control zone includes control line C, and test section includes detection line T.
The color depth of the existing test section of T wire bodies, the concentration of material to be checked, the depth of T line colors and thing to be checked in T lines reaction sample
The proportional relation of concentration of matter, the concentration in the deeper representative sample of color is higher.Extraneous ambient light has many differences, but
T lines and C lines are under same ambient light, and the color depth that C lines are represented is standard color depth, the existing control zone of C wire bodies
Color depth, so as to using C lines as with reference to the color depth that can accurately draw T lines.
Identification code 201 can be such as Quick Response Code, bar code or other be used for recognize coding.Identification code 201 is used to distinguish
The type and batch information of test paper.Additionally, identification code 201 may be located at the optional position in test strips.For example, identification code 201
May reside in strip, on the shell of test strips, in packaging bag, on packing box, or in certain annex.
Test strips data detection device of the present utility model has the function of being calibrated to result based on environment temperature.Phase
For visually observing judged result, user can not only obtain the qualitative results of more accurately test paper, can also realize quantitative
Detection, and professional equipment need not be relied on, can more obtain diagnostic result and the suggestion of specialty.
And, test strips data detection device of the present utility model environment temperature not to external world carries out artificial intervention,
Without device as using similar constant temperature, incubating, but amendment different temperatures is caused to testing result by way of calibration
Influence, using the standard curve of different temperatures in memory cell, or the amendment to preset standard curve, or according to temperature value
With concentration of specimens value, the standard curve of the concentration value being fitted by computing ensures the accuracy of testing result, realizes high precision
Property and highly sensitive detection.
Embodiment
Following examples are detection at different temperatures to different samples, in actually detected, can be in different temperatures
Different standard curves are called, if actually detected temperature does not have corresponding standard curve, detection temperature and existing mark is chosen
The minimum standard curve of the absolute value of directrix curve temperature gap, calculates sample concentration.
Following examples detection sample standard curve temperature interval for 10 DEG C, 5 DEG C, 4 DEG C, 3 DEG C, 2 DEG C, 1 DEG C,
0.5 DEG C, lowest detection temperature is -40 DEG C, -30 DEG C, -25 DEG C, -20 DEG C, -15 DEG C, -10 DEG C, -5 DEG C of equitemperatures, Supreme Procuratorate's thermometric
Spend is 50 DEG C, 45 DEG C, 40 DEG C, 37 DEG C, 35 DEG C, 30 DEG C, 25 DEG C of isothermal angle value.Generation is only chosen by length limits following examples
The several temperature values of table illustrate influence of the temperature to detected value, and the standard curve that different temperatures is transferred.
Embodiment 1：Detection of the different temperatures to H-FABP
Using H-FABP (hFABP) immue quantitative detection reagent box (lot number：20160602) respectively 10
DEG C, 25 DEG C, react and detect in 45 DEG C of environment, detect sample concentration be respectively 50ng/mL, 25ng/mL, 12.5ng/mL,
3.125ng/mL, each concentration point detect 2 parallel cards, calculate T/C averages.Testing result is shown in Table 1.
The T/C values of hFABP are detected under the different temperatures of table 1 and concentration
According to the result of table 1 with concentration as x-axis, T/C values are mapped for y-axis, obtain the reaction song of hFABP under condition of different temperatures
Line, is shown in Fig. 4：
As can be seen from the figure influence of the temperature to T/C values is very big.The figure of taking out stitches that wherein temperature is 10 DEG C is chosen, enters line number
According to fitting, curvilinear equation is obtained.
Equation：Y=(A-D)/[1+ (x/C)B]+D
A=1.30507
B=-1.21543
C=27.40012
D=0.00222
r2=0.99979
HFABP is in 10 DEG C of standard curve such as Fig. 5 of fitting.
Embodiment 2：Testing sample cardiac muscle troponin I concentration is calculated using temperature calibration curve
Prepare cardiac muscle troponin I (cTnI) 50ng/mL, 25ng/mL, 12.5ng/mL, 3.125ng/mL, 0.39ng/
ML, 0ng/mL normal concentration sample, using cTnI immue quantitative detection reagent box (lot numbers：20160101) it is interval with 0.5 DEG C, 10
Determine DEG C to reaction in 45 DEG C of environment and respectively the sample of above series concentration (it is equal that each concentration point detects that 2 parallel card are calculated
Value), draw the standard curve of T/C and concentration.Testing result is shown in Table 2.
The cTnI thermograde T/C measured values of table 2
The (Continued) of table 2
The (Continued) of table 2
The (Continued) of table 2
The (Continued) of table 2
The (Continued) of table 2
The (Continued) of table 2
The (Continued) of table 2
T/C concentration standard curves are drawn by taking 21 DEG C of results as an example.With T/C values as x-axis, concentration ng/mL is that y-axis is fitted song
Line, data fitting is carried out with " multinomial " model of fit in Excel " addition Trendline ", obtains the standard of cTnI at 21 DEG C
Curve and its regression equation, standard curve are shown in attached lower Fig. 6.When sample to be tested is detected under 21 DEG C of environment, instrument is adjusted
Calculated with above standard curve, such as testing sample T/C values at 21 DEG C are 0.37, call the curvilinear equation (y=
60.801x2+ 28.65x-0.2527) substitute into after be calculated concentration value and be：18.67ng/ml.
Embodiment 3：The concentration of testing sample heart Ck-MM is calculated by way of temperature coefficient
Using heart Ck-MM (CKMB) immue quantitative detection reagent box (lot number：20151107) respectively 10
DEG C, 25 DEG C, react and detect in 45 DEG C of environment, detect concentration such as the following table of sample, each concentration point 2 parallel cards of detection,
The T/C values of detection are recorded, temperature coefficient (with the T/C values under detection temperature divided by the T/C values at 25 DEG C) and temperature coefficient is calculated
Average.Testing result is shown in Table 3.
The T/C values of CKMB are detected under the different temperatures of table 3, concentration
CKMB is shown in accompanying drawing 7 in 25 DEG C of matched curves, and with concentration ng/mL as x-axis, T/C values are y-axis.Curvilinear equation：
Equation：Sigmoidal, Logistic, 4Parameter
Formula：F=if (x ＜=0, if (b ＜ 0, y0, y0+a), if (b ＞ 0, y0+a/ (1+abs (x/x0) ^b), y0+a*
abs((x/x0))^(abs(b))/(1+(abs(x/x0))^(abs(b)))))
Parameter：
a 1.5755
b -1.0826
x0 28.8177
y0 0.0267
Note：" * " is represented and is multiplied by, and " ^ " represents superscript.
Temperature and temperature coefficient matched curve are equations：Polynomial, Quadratic
Formula：F=y0+a*x+b*x^2
Parameter：
y0 0.6575
a 0.0144
B -3.0000E-005 (as -0.00003)
For example, when temperature is 45 DEG C, it is 1.689 to measure T/C values, then, temperature coefficient formula is brought into by 45 DEG C, obtains temperature
Coefficient is 1.225, and by coefficient computing, 1.689/1.225=1.379, computing formula when bringing 25 DEG C into by 1.379 draws
Actual concentrations are 150ng/ml.
By temperature and temperature coefficient, both curves are shown in that Fig. 8 and functional relation are as follows, and wherein abscissa X is temperature DEG C,
Ordinate is temperature coefficient, and temperature coefficient is the amendment carried out to preset standard curve.In practical operation, by detection temperature,
Calculate temperature coefficient；The T/C values that the T/C values of detection are corrected divided by temperature coefficient, then bring 25 DEG C of standard curves into, obtain
Go out the concentration value of correction.
Above example is used for the purpose of clearly demonstrating done citing, not to the restriction of implementation method.
Claims (10)
1. a kind of test strips data detection device, it is characterised in that the detection means includes consisting of part：
I) signal collecting element,
Ii) data processing equipment, wherein the data processing equipment further includes following component：
Ii-1) input element,
Ii-2) the memory cell being connected with the input element,
Ii-3) the analytic unit being connected with the memory cell, and
Ii-4) the output element being connected with the analytic unit,
Iii) temperature acquisition element,
Wherein, the signal collecting element and the temperature acquisition element respectively connect described in the data processing equipment
Input element.
2. test strips data detection device according to claim 1, it is characterised in that the input element, memory cell, analysis
One or more in unit, output element and temperature acquisition element are positioned at the inside of the detection means.
3. test strips data detection device according to claim 2, it is characterised in that the input element, memory cell, analysis
Unit, output element and temperature acquisition element are respectively positioned on the inside of the detection means.
4. test strips data detection device according to claim 1, it is characterised in that the input element, memory cell, analysis
One or more in unit, output element and temperature acquisition element are positioned at the outside of the detection means.
5. test strips data detection device according to claim 4, it is characterised in that the input element, memory cell, analysis
Unit, output element and temperature acquisition element are respectively positioned on the outside of the detection means.
6. test strips data detection device according to claim 1, it is characterised in that the signal collecting element includes optical signal
Acquisition elements, magnetic signal acquisition element, electrical signal collection element or picture signal acquisition elements.
7. test strips data detection device according to claim 1, it is characterised in that the signal collecting element includes shooting
Head.
8. according to the test strips data detection device of claim any one of 1-7, it is characterised in that the signal collecting element is adopted
Data of the collection from test strips to be detected, wherein the test strips include cog region, control zone and test section.
9. test strips data detection device according to claim 1, it is characterised in that the temperature acquisition element is TEMP
Device.
10. test strips data detection device according to claim 1, it is characterised in that the memory cell is hard disk, storage card
With one or more in RAM card.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561072A (en) * | 2017-09-14 | 2018-01-09 | 深圳泰首智能技术有限公司 | The accurate interpretation method and its interpretation device for realizing PUC T test results |
| CN111190004A (en) * | 2020-01-10 | 2020-05-22 | 上海泰辉生物科技有限公司 | Instant detection system for immunochromatography test strip |
-
2016
- 2016-09-02 CN CN201621036583.4U patent/CN206270251U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561072A (en) * | 2017-09-14 | 2018-01-09 | 深圳泰首智能技术有限公司 | The accurate interpretation method and its interpretation device for realizing PUC T test results |
| CN107561072B (en) * | 2017-09-14 | 2020-02-11 | 深圳泰首智能技术有限公司 | Interpretation method and interpretation device for accurately realizing PUC-T test result |
| CN111190004A (en) * | 2020-01-10 | 2020-05-22 | 上海泰辉生物科技有限公司 | Instant detection system for immunochromatography test strip |
| CN111190004B (en) * | 2020-01-10 | 2023-11-10 | 上海泰辉生物科技有限公司 | Instant detection system of immunochromatography test strip |
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Effective date of registration: 20200602 Address after: 225300 Jiangsu city of Taizhou province China Road West of Lu Jia Lu, Chengkou pharmaceutical G59 East Building 63, one to three layers of the West Co-patentee after: Kunming lianen Biotechnology Co.,Ltd. Patentee after: JIANGSU DAJUN BIOTECHNOLOGY Co.,Ltd. Address before: 225300 Jiangsu city of Taizhou province China Road West of Lu Jia Lu, Chengkou pharmaceutical G59 East Building 63, one to three layers of the West Patentee before: JIANGSU DAJUN BIOTECHNOLOGY Co.,Ltd. |
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