CN106124778A - Immunoblotting assay instrument - Google Patents
Immunoblotting assay instrument Download PDFInfo
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- CN106124778A CN106124778A CN201610603365.2A CN201610603365A CN106124778A CN 106124778 A CN106124778 A CN 106124778A CN 201610603365 A CN201610603365 A CN 201610603365A CN 106124778 A CN106124778 A CN 106124778A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
The invention discloses a kind of immunoblotting assay instrument, belong to the technical scheme in analyser field, including casing, in casing, be provided with mould bar loading plate, rail mechanism, image mechanism, illumination mechanism and main control board；Described casing is provided with through-hole groove, the shell wall of the through casing of through-hole groove；Described mould bar loading plate is actively coupled with rail mechanism, and mould bar loading plate can move on rail mechanism, and mould bar loading plate can move to outside casing through through-hole groove；Described image mechanism is located at the top of mould bar loading plate, and mould bar loading plate is placed in the coverage of image mechanism；Quasi-mode bar loading plate, mould bar loading plate are placed in the range of exposures of illumination mechanism by described illumination mechanism；Described main control board and image mechanism, illumination mechanism are electrically connected with, and main control board is used for controlling image mechanism and carries out shooting and illumination mechanism carries out light filling.In sum, integrated level of the present invention is high, volume is little, be easy to carry, and solves the problem that prior art cannot carry use.
Description
Technical field
The present invention relates to a kind of analyser, particularly to a kind of immunoblotting assay instrument.
Background technology
Immunoblotting (immunoblotting), also known as Western blotting (Western blotting), is to resist according to antigen
The method of certain albumen in the specific binding detection of complex sample of body.This method is in gel electrophoresis and solid-phase immunoassay skill
A kind of new immune biochemical technology grown up on the basis of art.Owing to immunoblotting has the high resolution of SDS-PAGE with solid
The high specific of phase immunoassay and sensitivity, become a kind of routine techniques of analysis of protein.Immunoblotting is usually used in mirror
Certain albumen fixed, and albumen can be carried out quantitative and semi-quantitative analysis, in conjunction with chemiluminescence detection, multiple sample can be compared simultaneously
The expression difference of product same protein.Immunoblotting is by resisting that antibody and the target protein being attached to solid support are presented
Former epi-position generation specific immune response detects, and whole process includes: the separation of protein, electrotransfer, immunology detection
Parts several with signal detection.
At present, on market, the instrument detection device of immunoblotting is the hugest, expensive, it is impossible to carry for user
Use, bring great inconvenience for user.
Summary of the invention
It is an object of the invention to provide a kind of immunoblotting assay instrument, to solve existing immunoblotting assay instrument volume
Greatly, the problem that use cannot be carried.
In order to solve above-mentioned technical problem, the invention provides a kind of immunoblotting assay instrument,
Including casing, in described casing, it is provided with mould bar loading plate, rail mechanism, image mechanism, illumination mechanism and master control electricity
Road plate；
Described casing is provided with through-hole groove, the shell wall of the through described casing of described through-hole groove；
Described mould bar loading plate is actively coupled with described rail mechanism, and described mould bar loading plate can be at described rail mechanism
Upper movement, and described mould bar loading plate can move to outside described casing through described through-hole groove；
Described image mechanism is located at the top of described mould bar loading plate, and described mould bar loading plate is placed in described image mechanism
In coverage；
Described illumination mechanism is directed at described mould bar loading plate, and described mould bar loading plate is placed in the irradiation model of described illumination mechanism
In enclosing；
Described main control board is electrically connected with described image mechanism, described illumination mechanism, and described main control board is used for
Control described image mechanism to shoot, and described main control board is additionally operable to control described illumination mechanism and carries out light filling.
Preferably, being additionally provided with elastic force snap close in described casing, described mould bar loading plate one end is relative with described through-hole groove, institute
Stating the mould bar loading plate other end relative with described elastic force snap close, described mould bar loading plate can shift to described elastic force snap close and described
Through-hole groove, one end that described mould bar loading plate is relative with described elastic force snap close is provided with latch, and described latch can be with described elastic force
Snap close locking and separation.
Preferably, one end that described mould bar loading plate is relative with described elastic force snap close is provided with mounting groove, and described latch is installed
In described mounting groove.
Preferably, it is additionally provided with installing plate, swing arm, gangbar and extension spring in described casing；
Described installing plate is placed in the side of described mould bar loading plate；
One end of described swing arm couples with described installing plate rotating shaft, and the other end of described swing arm is provided with waist hole, described
Swing arm is provided with the one end in described waist hole and can move toward described through-hole groove and described elastic force snap close；
Described gangbar one end couples with described mould bar loading plate, and the described gangbar other end inserts in described waist hole；
One end of described extension spring couples with described installing plate, and the other end of described extension spring couples with described swing arm, described
Extension spring is for pulling to described through-hole groove by described swing arm.
Preferably, described installing plate is provided with antivibrator, and described antivibrator is provided with the pin that can stretch out and regain, described
Pin abuts with described swing arm, and described antivibrator shifts to the speed of described through-hole groove for reducing described swing arm.
Preferably, described rail mechanism is provided with gathering sill, and described gathering sill is along the motion track of described mould bar loading plate
Arrange；
Described mould bar loading plate is provided with guide pad, and described guide pad embeds in described gathering sill, and described guide pad can
Move in described gathering sill.
Preferably, described illumination mechanism is located between described mould bar loading plate and described image mechanism, described illumination mechanism
It is provided with penetrating region, two surfaces of the through described illumination mechanism in described penetrating region, described illumination mechanism and described mould bar loading plate phase
To a surface be provided with light compensating lamp, described image mechanism is directed at described penetrating region.
Preferably, described immunoblotting assay instrument also includes baffle plate, and one end of described mould bar loading plate passes described through hole
Groove couples with described baffle plate, and described baffle plate is for hiding described through-hole groove.
Preferably, described main control board is electrically connected with USB interface, and described USB interface is located at outside described casing.
Preferably, in described casing, be provided with two pieces of barricades, described main control board be located at two pieces of described barricades it
Between.
Beneficial effects of the present invention is as follows:
In the prior art, immunoblotting assay instrument have hatch, liquid feeding, drying, result scanning, result output etc. multiple
Function, so immunoblotting assay instrument needs to arrange control device, support means, peristaltic-type pump arrangement, shaking table device, be dried simultaneously
Device, liquid sucking device, camera head and light compensating apparatus, this will cause immunoblotting assay instrument volume excessive, it is impossible to carries use,
Inconvenience is brought for using；It may be noted that the premiere feature utilizing immunoblotting assay instrument is to obtain scanning result, hatch, add
The function such as liquid, drying can be realized by other auxiliary equipments, so the present invention has abandoned multiple non-essential function, only at machine
Mould bar loading plate, image mechanism, illumination mechanism and main control board are set in shell, and described image mechanism is located at mould bar loading plate
Top, mould bar loading plate is placed in the coverage of image mechanism, and described illumination mechanism is also directed at described mould bar loading plate,
So when the present invention works, testing sample will be placed on mould bar loading plate, then image mechanism is on quasi-mode bar loading plate
Testing sample be scanned detection, during Scanning Detction, illumination mechanism carries out light filling, obtains scanning result with this；
Owing to no longer arranging the parts such as peristaltic-type pump arrangement, shaking table device, drying device and liquid sucking device, therefore immunoblotting assay instrument becomes
Obtaining small and exquisite portable, the use for user brings great convenience.
Accompanying drawing explanation
In order to be illustrated more clearly that technical scheme, the accompanying drawing used required in embodiment will be made below
Introduce simply, it should be apparent that, the accompanying drawing in describing below is only some embodiments of the present invention, general for this area
From the point of view of logical technical staff, on the premise of not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the immunoblotting assay instrument structural representation that the preferred embodiment for the present invention provides；
Fig. 2 is that schematic diagram one disassembled by the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 3 is that schematic diagram two disassembled by the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 4 is that schematic diagram three disassembled by the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 5 is that schematic diagram four disassembled by the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 6 is that schematic diagram five disassembled by the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 7 is the track plates structural representation of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 8 is the mould bar loading plate structural representation of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Fig. 9 is the latch structure schematic diagram of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Figure 10 is the snap lock buckle structure schematic diagram of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Figure 11 is the illumination mechanism structure schematic diagram of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Figure 12 is the swing arm structural representation of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Figure 13 is the damper structure schematic diagram of the immunoblotting assay instrument that the preferred embodiment for the present invention provides；
Figure 14 is the casing structure schematic diagram of the immunoblotting assay instrument that the preferred embodiment for the present invention provides.
Reference is as follows:
1, casing；11, through-hole groove；
2, mould bar loading plate；21, sample deposition；22, latch；23, elastic force snap close；24, mounting groove；25, guide pad；
26, baffle plate；
3, image mechanism；
4, illumination mechanism；41, penetrating region；42, light compensating lamp；
5, main control board；
61, track plates；62, track；63, sliding shoe；64, gathering sill；
71, installing plate；72, swing arm；721, waist hole；73, gangbar；74, extension spring；75, antivibrator；751, pin；
8, barricade.
Detailed description of the invention
Below in conjunction with the accompanying drawing in embodiment of the present invention, the technical scheme in embodiment of the present invention is carried out clearly
Chu, it is fully described by.
Knowable to Fig. 1 is to 14, immunoblotting assay instrument of the present invention, including casing 1, in described casing 1, it is provided with mould bar
Loading plate 2, rail mechanism, image mechanism 3, illumination mechanism 4 and main control board 5；Described casing 1 is provided with through-hole groove 11, institute
State the shell wall of the through described casing 1 of through-hole groove 11；Described mould bar loading plate 2 is actively coupled with described rail mechanism, described mould bar
Loading plate 2 can move on described rail mechanism, and described mould bar loading plate 2 can move to described through described through-hole groove 11
Outside casing 1；Described image mechanism 3 is located at the top of described mould bar loading plate 2, and described mould bar loading plate 2 is placed in described video camera
In the coverage of structure 3；Described illumination mechanism 4 is directed at described mould bar loading plate 2, and described mould bar loading plate 2 is placed in described illumination
In the range of exposures of mechanism 4；Described main control board 5 is electrically connected with described image mechanism 3, described illumination mechanism 4, described master
Control circuit board 5 is used for controlling described image mechanism 3 and shoots, and described main control board 5 is additionally operable to control described illumination machine
Structure 4 carries out light filling.
In the prior art, immunoblotting assay instrument have hatch, liquid feeding, drying, result scanning, result output etc. multiple
Function, so immunoblotting assay instrument needs to arrange control device, support means, peristaltic-type pump arrangement, shaking table device, be dried simultaneously
Device, liquid sucking device, camera head and light compensating apparatus, this will cause immunoblotting assay instrument volume excessive, it is impossible to carries use,
Inconvenience is brought for using；It may be noted that the premiere feature of immunoblotting assay instrument is to obtain scanning result, hatch, liquid feeding, baking
The function such as dry can be realized by other auxiliary equipments, so the present invention has abandoned multiple non-essential function, only in casing 1
Mould bar loading plate 2, image mechanism 3, illumination mechanism 4 and main control board 5 are set, and described image mechanism 3 is located at mould bar and is loaded
The top of plate 2, mould bar loading plate 2 is placed in the coverage of image mechanism 3, and described illumination mechanism 4 is also directed at described mould bar
Loading plate 2, so when the present invention works, testing sample will be placed on mould bar loading plate 2, and then image mechanism 3 is to quasi-mode
Testing sample on bar loading plate 2 is scanned detection, and during Scanning Detction, illumination mechanism 4 carries out light filling, obtains with this
Obtain scanning result；Owing to no longer arranging the parts such as peristaltic-type pump arrangement, shaking table device, drying device and liquid sucking device, immunoblotting
Therefore analyser becomes small and exquisite portable, and the use for user brings great convenience.
Wherein, described mould bar loading plate 2 is mainly used in loading testing sample, so as shown in Figure 8, can load at mould bar
Plate 2 surface indentation forms sample deposition 21, and during being scanned detection, testing sample will be placed on to be placed at sample
In district 21, testing sample is realized fixing with this.
It addition, described rail mechanism is mainly used in support mould bar loading plate 2, and make the mould bar loading plate 2 can be at track
Carry out the movement that can come and go in mechanism, and one of which preferably embodiment is as it is shown in figure 5, described rail mechanism includes track
Plate 61, track 62 and sliding shoe 63, the platy structure that described track plates 61 is generally rectangular, track plates is located at by described track 62
On 61, and track 62 is along the length direction layout of track plates 61, and described sliding shoe 63 fastens with track 62 and couples, so that sliding
Block 63 can move along the layout track of track 62, and described mould bar loading plate 2 is then connected and fixed with sliding shoe 63, so that
Mould bar loading plate 2 is capable of way moving, if with Fig. 5 as reference, then it represents that mould bar loading plate 2 can move forward and backward
Dynamic.
Furthermore, described image mechanism 3 is located at the top of mould bar loading plate 2, and mould bar loading plate 2 is placed in the bat of image mechanism 3
In the range of taking the photograph, and the coverage moment of non-limiting image mechanism 3 is to quasi-mode bar loading plate 2, as long as when being scanned detection,
Quasi-mode bar loading plate 2 can just may be used by image mechanism 3；Such as, when mould bar loading plate 2 extend out to casing 1 outer time, image mechanism 3
The most relative with mould bar loading plate 2, and after testing sample is placed on mould bar loading plate 2, mould bar loading plate 2 will be back to casing 1
In, now image mechanism 3 is by being directed at the testing sample on mould bar loading plate 2, realizes Scanning Detction with this.
Further, described illumination mechanism 4 is placed in the photograph of illumination mechanism 4 to quasi-mode bar loading plate 2, mould bar loading plate 2
In the range of penetrating, and the range of exposures moment of non-limiting illumination mechanism 4 is to quasi-mode bar loading plate 2, as long as when being scanned detection,
Quasi-mode bar loading plate 2 can just may be used by illumination mechanism 4；Such as, when mould bar loading plate 2 extend out to casing 1 outer time, illumination mechanism 4
The most relative with mould bar loading plate 2, and after testing sample is placed on mould bar loading plate 2, mould bar loading plate 2 will be back to casing 1
In, now illumination mechanism 4, by being directed at the testing sample on mould bar loading plate 2, realizes illumination with this.
Finally, described main control board 5 is located in casing 1, as it is shown on figure 3, described main control board 5 is preferably disposed to casing
Bottom in 1, increases the weight bottom casing 1 with this, and the center of gravity keeping immunoblotting assay instrument is placed below, it is to avoid Diagnosis of Sghistosomiasis
Mark analyser is easily toppled over.
The preferred embodiment of the present invention, as shown in Fig. 3,5,10 and 14, is additionally provided with elastic force snap close 23, institute in described casing 1
State mould bar loading plate 2 one end relative with described through-hole groove 11, described mould bar loading plate 2 other end and described elastic force snap close 23 phase
Right, described mould bar loading plate 2 can shift to described elastic force snap close 23 and described through-hole groove 11, and described mould bar loading plate 2 is with described
One end that elastic force snap close 23 is relative is provided with latch 22, and described latch 22 can lock with described elastic force snap close 23 and separate.
When being scanned testing sample, image mechanism 3 needs to keep being directed at, if now detecting environment with testing sample
There are vibrations, then mould bar loading plate 2 may be caused to offset, i.e. image mechanism 3 and testing sample produces position deviation, thus
The error causing Scanning Detction is relatively big, even Scanning Detction failure；In order to solve this problem, present embodiment is in casing 1
It is additionally arranged elastic force snap close 23, is provided with latch 22 in mould bar loading plate 2 one end relative with elastic force snap close 23, so when mould bar loads
When elastic force snap close 23 shifted to by plate 2, latch 22 will insert in elastic force snap close 23, i.e. secure the position of mould bar loading plate 2, be now
Generation vibrations are made also to be not result in that image mechanism 3 produces position deviation with testing sample；It may be noted that described elastic force snap close 23 is
A kind of parts that can automatically lock and unlock, when latch 22 inserts elastic force snap close 23 for the first time, elastic force snap close 23 can be opened
Unlocking port, in order to latch 22 enters in fore shaft, after latch 22 inserts fore shaft, recovery is tightened up by fore shaft, real to latch 22 with this
Now locking, now pressing bolt 22, elastic force snap close 23 will apply thrust to fore shaft, and fore shaft will open again, and be pushed away by latch 22
Go out outside fore shaft, thus realize the unblock of latch 22.
In sum, application elastic force snap close 23 and latch 22 are not only able to realize the locking of mould bar loading plate 2, moreover it is possible to be easy to
Mould bar loading plate 2 is automatically popped up outside casing 1, and the use for user offers convenience.
The preferred embodiment of the present invention as shown in Fig. 3,5,8 and 9, described mould bar loading plate 2 and described elastic force snap close 23
Relative one end is provided with mounting groove 24, and described latch 22 is arranged in described mounting groove 24.
Along with the growth of the time of use, damaging, in order to tackle this situation, this reality easily occurs in the latch 22 repeatedly plugged
Mode of executing is provided with mounting groove 24 in mould bar loading plate 2 one end relative with elastic force snap close 23, and latch 22 is arranged on mounting groove
In 24, it is i.e. demountable structure between latch 22 and mould bar loading plate 2, if latch 22 damages, only latch 22 need to be replaced
Just may be used.
The preferred embodiment of the present invention, as shown in Fig. 4,12,14, is additionally provided with installing plate 71, swing arm in described casing 1
72, gangbar 73 and extension spring 74；Described installing plate 71 is placed in the side of described mould bar loading plate 2；One end of described swing arm 72
Coupling with the rotating shaft of described installing plate 71, the other end of described swing arm 72 is provided with waist hole 721, and described swing arm 72 is provided with described waist
The one end in hole 721 can be moved toward described through-hole groove 11 and described elastic force snap close 23；Described gangbar 73 one end and described mould bar
Loading plate 2 couples, and described gangbar 73 other end inserts in described waist hole 721；One end of described extension spring 74 and described installing plate
71 couple, and the other end of described extension spring 74 couples with described swing arm 72, and described extension spring 74 is for pulling to described swing arm 72
Described through-hole groove 11.
During adding testing sample, need to be drawn out to outside casing 1 mould bar loading plate 2, and add elastic force snap close
After 23, although mould bar loading plate 2 can automatic spring, but the distance ejected is shorter, so user also needs manually to be loaded by mould bar
Plate 2 is completely pulled out, and user operation is experienced not good enough, so for the operating experience improving user, the most effectively pulling out mould bar and load
Plate 2 is particularly important；In order to realize this beneficial effect, present embodiment is additionally arranged installing plate 71, swing arm 72, gangbar 73 and draws
Spring 74, swing arm 72 can swing on installing plate 71, and gangbar 73 realizes coupling of mould bar loading plate 2 and swing arm 72, and
Realized the pull-out of mould bar loading plate 2 by extension spring 74, concrete principle is as follows: when elastic force snap close 23 locking latch 22, swing arm 72
The one end being provided with waist hole 721 will move to elastic force snap close 23, as shown in Figure 4, owing to extension spring 74 is placed in swing arm 72 and through-hole groove 11
Between, so extension spring 74 now will be in extended state, and after elastic force snap close 23 separates with latch 22, extension spring 74 pulls on pendulum
Lever 72 is provided with the one end in waist hole 721 and shifts to through-hole groove 11, so mould bar loading plate 2 will be executed by swing arm 72 by gangbar 73
Power, so that mould bar loading plate 2 can automatically, fully extend out to outside casing 1, brings more preferable operating experience for user.
The preferred embodiment of the present invention is such as Fig. 4, shown in 13 and 14, and described installing plate 71 is provided with antivibrator 75, described
Antivibrator 75 is provided with the pin 751 that can stretch out and regain, and described pin 751 abuts with described swing arm 72, described antivibrator
75 are used for reducing described swing arm 72 shifts to the speed of described through-hole groove 11.
After mould bar loading plate 2 is pulled out by application extension spring 74, although ensure that mould bar loading plate 2 can be completely pulled out,
But owing to the pulling force of extension spring 74 is relatively big, the speed causing mould bar loading plate 2 to pull out, not only it is easily damaged each dependent part
Part, the testing sample being also easily caused on mould bar loading plate 2 turns up and drops；In order to solve this problem, present embodiment is in peace
Being additionally arranged antivibrator 75 on dress plate 71, the pin 751 of antivibrator 75 abuts with swing arm 72, reduces swing arm 72 with this and shifts to lead to
The speed of hole slot 11, concrete principle is as follows: after latch 22 is unlocked by elastic force snap close 23, and extension spring 74 pulls on swing arm 72 toward logical
Hole slot 11 moves, as shown in Figure 4, owing to antivibrator 75 is placed between swing arm 72 and through-hole groove 11, so antivibrator 75 is by right
The part pulling force that extension spring 74 applies is offset, and now pin 751 will slowly be retracted in antivibrator 75, and in like manner, swing arm 72 also will
Slowly shift to through-hole groove 11, thus realize the slow pull-out of mould bar loading plate 2, it is ensured that each associated components is not easy because of pull-out behaviour
Make and damage, also avoid testing sample to turn up and drop.
As best shown in figures 5 and 7, described rail mechanism is provided with gathering sill 64, described guiding to the preferred embodiment of the present invention
Groove 64 is arranged along the motion track of described mould bar loading plate 2；Described mould bar loading plate 2 is provided with guide pad 25, described guide pad
25 embed in described gathering sill 64, and described guide pad 25 can move in described gathering sill 64.
Testing sample is the highest with image mechanism 3 degree of registration, and the result of Scanning Detction is the most accurate, but mould bar loading plate 2
Process in movement produces position deviation unavoidably, so how reduces position deviation particularly important；In order to solve this problem, this
Embodiment arranges gathering sill 64 on rail mechanism, arranges guide pad 25 on mould bar loading plate 2, and guide pad 25 embeds and guides
In groove 64, one of them preferably embodiment as best shown in figures 5 and 7, described gathering sill 64 is located on track plates 61, and gathering sill
64 is parallel with track 62 relative, so when mould bar loading plate 2 moves, gathering sill 64 and track 62 also define mould bar and load
The moving direction of plate 2, thus decrease the position deviation produced in mould bar loading plate 2 moving process, improve Scanning Detction knot
The accuracy of fruit.
It addition, knowable to Fig. 4 and 7, described gathering sill 64 also can be by through for track plates 61, and now guide pad 25 will be through leading
To groove 64 so that the end of guide pad 25 extends to the lower section of track plates 61, and described gangbar 73 is by the end with guide pad 25
Portion couples, and the structure that this kind of connecting mode enables to immunoblotting assay instrument is compacter, for reducing immunoblotting assay instrument
Volume provide probability.
The preferred embodiment of the present invention as shown in figs. 3 and 11, described illumination mechanism 4 be located at described mould bar loading plate 2 with
Between described image mechanism 3, described illumination mechanism 4 is provided with penetrating region 41, the two of the through described illumination mechanism 4 in described penetrating region 41
Surface, described illumination mechanism 4 surface relative with described mould bar loading plate 2 is provided with light compensating lamp 42, and described image mechanism 3 is directed at
Described penetrating region 41.
Owing to the free space in casing 1 is limited, so the structural compactness improving immunoblotting assay instrument is particularly important；
In order to realize this purpose, illumination mechanism 4 is located between mould bar loading plate 2 and image mechanism 3 by present embodiment, it is to avoid mould bar
Space between loading plate 2 and image mechanism 3 is idle, is provided with penetrating region 41, the through light in penetrating region 41 due to illumination mechanism 4 again
According to two surfaces of mechanism 4, so the testing sample on mould bar loading plate 2 can be swept by image mechanism 3 through penetrating region 41
Retouch detection, it is ensured that Scanning Detction can be normally carried out.
The preferred embodiment of the present invention is such as Fig. 1, shown in 6 and 14, and described immunoblotting assay instrument also includes baffle plate 26, institute
The one end stating mould bar loading plate 2 couples through described through-hole groove 11 with described baffle plate 26, and described baffle plate 26 is for described through hole
Groove 11 hides.
In actual application, in order to ensure the accurate of scanning result, it usually needs avoid the interference of ambient light, so
Image mechanism 3 and testing sample are placed in airtight casing 1, but in order to make mould bar loading plate 2 smooth and easy can pass through through-hole groove
11, the end of mould bar loading plate 2 needs less than the area of through-hole groove 11, and therefore ambient can be incident upon machine by via through holes groove 11
In shell 1, Scanning Detction is interfered；In order to solve this problem, present embodiment is additionally arranged baffle plate 26, mould bar loading plate 2
One end couple with baffle plate 26 through through-hole groove 11 baffle plate 26, so when mould bar loading plate 2 moves back in casing 1, baffle plate 26
To block outside through-hole groove 11, thus completely cut off injecting of ambient, the accuracy for Scanning Detction provides guarantee.
The preferred embodiment of the present invention is as follows, and described main control board 5 is electrically connected with USB interface, described USB interface
It is located at outside described casing 1.
USB interface is conventional data transmission interface, so present embodiment is by main control board 5 and USB interface electricity
Property connect so that immunoblotting assay instrument can be by USB interface output scanning result in diversified analytical equipment, greatly
Improve greatly the versatility of immunoblotting assay instrument.
The preferred embodiment of the present invention is as in figure 2 it is shown, be provided with two pieces of barricades 8, described governor circuit in described casing 1
Plate 5 is located between two pieces of described barricades 8.
Because electromagnetic interference can affect the scanning result accuracy of immunoblotting assay instrument, so present embodiment is in casing
It is additionally arranged two pieces of barricades 8 in 1, and main control board 5 is located between two barricades 8, thus prevent outside electromagnetic interference shadow
Ringing the normal work of main control board 5, the accuracy for immunoblotting assay instrument scanning result provides guarantee.
Certainly, each embodiment above-mentioned can be used alone, it is also possible to combination application, and the above is the excellent of the present invention
Select embodiment, it is noted that for those skilled in the art, in the premise without departing from the principle of the invention
Under, it is also possible to making some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (10)
1. an immunoblotting assay instrument, it is characterised in that
Including casing, in described casing, it is provided with mould bar loading plate, rail mechanism, image mechanism, illumination mechanism and main control board；
Described casing is provided with through-hole groove, the shell wall of the through described casing of described through-hole groove；
Described mould bar loading plate is actively coupled with described rail mechanism, and described mould bar loading plate can move on described rail mechanism
Move, and described mould bar loading plate can move to outside described casing through described through-hole groove；
Described image mechanism is located at the top of described mould bar loading plate, and described mould bar loading plate is placed in the shooting of described image mechanism
In the range of；
Described illumination mechanism is directed at described mould bar loading plate, and described mould bar loading plate is placed in the range of exposures of described illumination mechanism
In；
Described main control board is electrically connected with described image mechanism, described illumination mechanism, and described main control board is used for controlling
Described image mechanism shoots, and described main control board is additionally operable to control described illumination mechanism and carries out light filling.
Immunoblotting assay instrument the most according to claim 1, it is characterised in that be additionally provided with elastic force snap close in described casing,
Described mould bar loading plate one end is relative with described through-hole groove, and the described mould bar loading plate other end is relative with described elastic force snap close, institute
Stating mould bar loading plate and can shift to described elastic force snap close and described through-hole groove, described mould bar loading plate is relative with described elastic force snap close
One end be provided with latch, described latch can be tight with described snap lock padlock and separate.
Immunoblotting assay instrument the most according to claim 2, it is characterised in that described mould bar loading plate and described snap lock
Detaining relative one end and be provided with mounting groove, described latch is arranged in described mounting groove.
Immunoblotting assay instrument the most according to claim 2, it is characterised in that
Installing plate, swing arm, gangbar and extension spring it is additionally provided with in described casing；
Described installing plate is placed in the side of described mould bar loading plate；
One end of described swing arm couples with described installing plate rotating shaft, and the other end of described swing arm is provided with waist hole, described swing
Bar is provided with the one end in described waist hole and can move toward described through-hole groove and described elastic force snap close；
Described gangbar one end couples with described mould bar loading plate, and the described gangbar other end inserts in described waist hole；
One end of described extension spring couples with described installing plate, and the other end of described extension spring couples with described swing arm, described extension spring
For described swing arm is pulled to described through-hole groove.
Immunoblotting assay instrument the most according to claim 4, it is characterised in that described installing plate is provided with antivibrator, institute
Stating antivibrator and be provided with the pin that can stretch out and regain, described pin abuts with described swing arm, and described antivibrator is used for reducing
The speed of described through-hole groove shifted to by described swing arm.
Immunoblotting assay instrument the most according to claim 1, it is characterised in that
Described rail mechanism is provided with gathering sill, and described gathering sill is arranged along the motion track of described mould bar loading plate；
Described mould bar loading plate is provided with guide pad, and described guide pad embeds in described gathering sill, and described guide pad can be in institute
Move in stating gathering sill.
Immunoblotting assay instrument the most according to claim 1, it is characterised in that described illumination mechanism is located at described mould bar dress
Between support plate and described image mechanism, described illumination mechanism is provided with penetrating region, the two of the through described illumination mechanism in described penetrating region
Surface, a described illumination mechanism surface relative with described mould bar loading plate is provided with light compensating lamp, and described image mechanism alignment is described
Penetrating region.
Immunoblotting assay instrument the most according to claim 1, it is characterised in that described immunoblotting assay instrument also includes gear
Plate, one end of described mould bar loading plate couples through described through-hole groove with described baffle plate, and described baffle plate is for described through-hole groove
Hide.
Immunoblotting assay instrument the most according to claim 1, it is characterised in that
Described main control board is electrically connected with USB interface, and described USB interface is located at outside described casing.
Immunoblotting assay instrument the most according to claim 1, it is characterised in that be provided with two pieces of barricades in described casing,
Described main control board is located between two pieces of described barricades.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610603365.2A CN106124778B (en) | 2016-07-27 | 2016-07-27 | Immunoblotting assay instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610603365.2A CN106124778B (en) | 2016-07-27 | 2016-07-27 | Immunoblotting assay instrument |
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| CN106124778A true CN106124778A (en) | 2016-11-16 |
| CN106124778B CN106124778B (en) | 2018-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
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Cited By (1)
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| CN106680514A (en) * | 2017-03-27 | 2017-05-17 | 武汉优视科技有限公司 | Automatic detector for immunoblotting |
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| CN106124778B (en) | 2018-06-12 |
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