CN201681045U - Portable wireless transmission and image display type microorganism fast detection searchlight - Google Patents

Portable wireless transmission and image display type microorganism fast detection searchlight Download PDF

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Publication number
CN201681045U
CN201681045U CN2009203511821U CN200920351182U CN201681045U CN 201681045 U CN201681045 U CN 201681045U CN 2009203511821 U CN2009203511821 U CN 2009203511821U CN 200920351182 U CN200920351182 U CN 200920351182U CN 201681045 U CN201681045 U CN 201681045U
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China
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light source
beam splitter
wireless transmission
image display
searchlight
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Expired - Fee Related
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CN2009203511821U
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Chinese (zh)
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卢磊磊
李福生
卢淼淼
卢晶晶
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Abstract

The utility model discloses a portable wireless transmission and image display type microorganism fast detection searchlight, which belongs to the fields of biology, medical science, photoelectricity and information technology. The searchlight includes a light source generating means, a light source controlling means, a filter, a beam expanding mirror, a beam splitting mirror, an objective table, a holophote, a beam splitter, a signal intelligence analytical means, a wireless transmission module and a display screen. The light source controlling means comprises a collimation lens, a beam splitting mirror, a photoelectric diode and a control circuit. The output end of an intelligence chip is connected to the input end of the display screen and is also connected to the input end of the wireless module. A numeral hologram is generated through the interference between the sample light and the light passed through surrounding medium. Images, acquired through photosensitive component by users, are more objective, visual and more convenient to observe, moreover, data can be remotely transmitted in real time via the wireless module, preventing the data from losing and rewriting. The searchlight has the advantages of small volume, portable, wide application in various fields and conditions, as well as broad application perspective.

Description

Portable wireless transmission image display type microbial rapid detection searchlight
Technical field
The utility model discloses a kind of medical science, industry, environment of being used for and wait for that this institute of mark comprises the device that microorganism detects, counts and identifies, it specifically is a kind of portable wireless transmission image display type microbial rapid detection searchlight, belong to biomedical photoelectricity and areas of information technology, it realizes the non-destructive fast detecting of microorganism based on the digital hologram image-forming principle.
Background technology
Dutchman's Leeuwenhoek in 1676 discloses the microorganism world that a past never known with the self-control microscope.After this, people recognize microorganism in not only extensively existence of occurring in nature, huge amount, of a great variety, and closely bound up with the mankind's life.
Culture of microorganism is the classic method more than having continued a century.Traditional cultivation and decoration method, shortcoming is: complex operation and need are confined in the laboratory by hand, operating personnel's level professional technology is had relatively high expectations, and have artificial error.In addition, cultivation reaches the needed time of the visible bacterium colony of visual inspection and did not wait (specifically deciding on the kind of microorganism) at 1 day to tens days, and the workload of conventional colony counting is big, effort, time-consuming.And decoration method can pollute environment.Therefore, traditional method has been difficult to satisfy the microorganism detection that requires raising day by day.Along with development of science and technology, some researchists are devoted to develop some new technology or method to be applied to microorganism detection, so that realize the fast detecting to them.
Find by retrieval, publication number is that the Chinese patent of CN201262615Y discloses the utility model patent of a kind of " pathogenic microorganism pick-up unit " on June 24th, 2009, its principle is based on surface plasma body resonant vibration (SPR) biosensor technology, feature is: the metal surface is fixed with the special molecular of energy recognition property, compound form or the process of dissociating in, the refractive index of metallic film surface solution changes, the SRP sensor can detect such variation, and refraction realizes by a prism.Yet spr sensor detector sensitivity and noise level can just exert an influence to the resolution of system, and the change of solution refractive index also can be subjected to the influence of other factors.
Other detection techniques that grow up at present, such as the PNA probe technique, the firefly luciferase method, molecular biology is the detection method on basis, though detection methods such as flow cytometer have shortened detection time greatly than conventional traditional cultural method, improved detection efficiency, yet still there is deficiency in various degree in these technology, such as: the operating process complexity, small throughput, range of application is narrow, specificity is not high, instrument reagent costs an arm and a leg, need kill microorganisms and can not distinguish dead cell and living cells, some such as also need just can detect in special laboratory at limitation.Thereby limited the application of these technology in medical science, industry, environment etc., especially limited the production scene that presses for field monitoring and the application in the field environment.
The utility model content
Based on more than, the purpose of this utility model provides a kind of portable wireless transmission image display type microbial rapid detection searchlight, can carry out fast detecting, counting, evaluation and data in real time wireless transmission to the microorganism in medical science, industry, the environment to gathering, used the digital hologram image-forming principle that was widely studied in recent years to be the basis, hologram image adopts sensor acquisition, and image is transferred to externally connected with display screen or carries out the real-time Transmission of teledata by wireless module after intelligent chip is handled.The utility model has overcome the existing defective that has technology, has kept the advantage of traditional microbe culture, does not influence the separation of follow-up pure culture, further screenings such as evaluation and drug sensitivity assay.
The utility model is achieved through the following technical solutions, a kind of portable wireless transmission image display type microbial rapid detection searchlight, comprise: light source takes place and control device, in the light path of light source rear end optical filter is installed, beam expanding lens, beam splitter with inclined light shaft 45 degree, objective table, completely reflecting mirror with inclined light shaft 45 degree, beam splitter, the signal intelligent analytical equipment, wireless transport module and display screen, it is characterized in that: described light source takes place to comprise laser generator and control circuit thereof with control device, further, the platform in light source generating means and the housing is affixed; Described control device comprises collimating mirror, beam splitter, photodiode, control circuit.Wherein, photodiode output is connected with the input end of control circuit;
Further, described beam expanding lens front end is placed with that optical filter, beam splitter front end are placed with beam expanding lens, the sample that is positioned on the objective table 7 places under the irradiation of target beam, target beam through beam splitter enters into completely reflecting mirror 8, reference beam enters into completely reflecting mirror 10, reference beam and target beam converge interference imaging at the beam splitter place, the intellectual analysis device is handled interference image, sensor in it is to interference image, again through the intellectual analysis chip analysis, at last at display screen or computing machine place display image.
Described microbial rapid detection searchlight, it also comprises USB interface, wireless communication module, wherein USB interface connects with upper display screen or computer interface, makes things convenient for the user that detected data sync is shown, or data recording is stored; Wireless communication module is convenient to the testing result real-time Transmission to remote control center;
Described microbial rapid detection searchlight, the light source of its described light source generating means are the combination of one or more laser diodes, and preferred, generating device of laser is chosen as the He-Ne laser instrument;
Further, described microbial rapid detection searchlight also comprises the affixed of being connected of housing, base, power supply, reference beam passage and housing, light source generating means and inner shell, and control circuit for light source is connected with housing and lower device.
The utility model is achieved through the following technical solutions, and the utility model comprises: light source generation and control device, collimating mirror, optical filter, beam expanding lens, with the beam splitter of inclined light shaft 45 degree, with completely reflecting mirror, beam splitter, objective table, signal intelligent analytical equipment, wireless transport module, the display screen of inclined light shaft 45 degree.It is characterized in that: described beam expanding lens front end is placed with the sample that optical filter, beam splitter front end are placed with beam expanding lens, locate to place through the target beam irradiation objective table (7) of beam expanding lens beam splitter, enter completely reflecting mirror (8) and arrive beam splitter, another reference beam through beam splitter enters into another completely reflecting mirror (10), locate to interfere at beam splitter (9) with target beam, image in the intellectual analysis device; Described signal intelligent analytical equipment comprises sensor assembly and intellectual analysis chip (14), the output terminal of described sensor assembly with link to each other with the intelligent chip input end, the output terminal of intelligent chip is connected with input end and computer with display screen or by USB interface, and the input end of described wireless transport module links to each other with the output terminal of intelligent chip.
Further, described optical filter is placed between beam splitter (3) and the beam expanding lens (5);
Further, described sensor processing module is CMOS or ccd sensor;
Further, described CMOS or ccd sensor and intellectual analysis chip portfolio are assemblied in the housing;
Further, described display screen links to each other by USB interface with the output terminal of intellectual analysis chip and exports in the display screen;
The utility model adopts the principle of digital hologram imaging to realize the imaging to determinand, use the laser illumination target, the light of the reference light source that scattered light can send with same laser instrument interferes, and the image of interference can be gone up the record interference pattern at photographic plate or digital sensor (for example ccd sensor).
The beneficial effects of the utility model and application thereof:
(1) changed the deficiency that traditional microbe culture needs for a long time could naked eyes to detect bacterium colony, greatly shortened the detection time of sample to be measured, quickened testing process, and the photo-sensitive cell images acquired is more objective, but identification result has been good;
(2) output by external display screen makes image document more directly perceived, observes more convenient;
(3) can not cause damage to microorganism or cell after after testing, microorganism after the detection or cell can continue on for the tests such as further evaluation of pure culture, drug sensitive test, microorganism or cell;
(4) laser control circuit guarantees the stability of output laser, the service time of having improved equipment.
The utility model detection sensitivity height, easy to use, can be applied to a plurality of fields, based on miniaturization Design, easy to carry, particularly can satisfy in corresponding occasion and make the on-the-spot needs that detect, and a data in real time remote transmission of gathering is to control center, avoided losing and being distorted of data, thereby demonstrated unique advantages, had broad application prospects in the environmental monitoring field.
Description of drawings
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples the utility model device is described in further detail, wherein:
Fig. 1 light signal of the present utility model transmits synoptic diagram;
Fig. 2 data processing of the present utility model and signal transmit synoptic diagram.
Embodiment
The 1-generating device of laser; The 2-collimating mirror; The 3-beam splitter; The 4-optical filter; The 5-beam expanding lens; The 6-beam splitter; The 7-objective table; The 8-completely reflecting mirror; The 9-beam splitter, the 10-completely reflecting mirror; The 11-photodiode; The 12-laser control circuit; The 13-sensor assembly; 14-intellectual analysis chip; The 15-wireless module; The 16-display screen.
Referring to accompanying drawing.Present embodiment is implemented under with the technical solution of the present invention prerequisite, has provided detailed embodiment and process, should be appreciated that drawings and Examples of the present invention only are used to explain that the utility model, protection domain are not limited to following embodiment.Being familiar with this operator is to be understood that, the modification that the technical solution of the utility model is carried out or be equal to replacement, make up and be applied to other purposes, only any possible modification all should be included in the spiritual scope of the present invention, also be encompassed in the claim scope of the present utility model, only be confined to the usefulness of claim.
Fig. 1 is that light signal of the present utility model transmits synoptic diagram; Fig. 2 is that data processing of the present utility model and signal transmit synoptic diagram.As Fig. 1, shown in 2, a kind of portable wireless transmission image display type microbial rapid detection searchlight, comprise: light source takes place and control device, in the light path of light source rear end optical filter is installed, beam expanding lens, beam splitter with inclined light shaft 45 degree, objective table, completely reflecting mirror with inclined light shaft 45 degree, beam splitter, the signal intelligent analytical equipment, wireless transport module and display screen, it is characterized in that: described beam expanding lens front end is placed with optical filter, the beam splitter front end is placed with beam expanding lens, target beam through the beam expanding lens beam splitter shines the sample that objective table (7) is located to place, enter completely reflecting mirror (8) and arrive beam splitter, another reference beam through beam splitter enters into another completely reflecting mirror (10), locate to interfere at beam splitter (9) with target beam, image in the intellectual analysis device; Described signal intelligent analytical equipment comprises sensor assembly and intellectual analysis chip (14), the output terminal of described sensor assembly with link to each other with the intelligent chip input end, the output terminal of intelligent chip is connected with input end and computer with display screen or by USB interface, and the input end of described wireless transport module links to each other with the output terminal of intelligent chip.
Wherein, described optical filter (4) is placed between beam splitter (3) and the beam expanding lens (5).
Wherein said light source takes place and control device comprises laser generator and control circuit, the light source of wherein said light source generating means is the combination of one or more laser diode laser instruments, preferably, the described light source generating means of present embodiment is the generating device of laser 1 of the light of emission 633nm wavelength, for example be the He-Ne laser instrument, this generating device of laser is fixed in the shell.Described device also comprises light source control device, from the light beam of generating device of laser 1 through collimating mirror again behind first beam splitter 3, part light enters light source control device, is electric signal through photodiode converts, and the control circuit chip is regulated and control to realize constant current the electric current of entire circuit.Another part light is successively through the beam splitter of optical filter, beam expanding lens directive and inclined light shaft 45 degree, and the sample that place at target beam directive objective table 7 places passes and enters total reflective mirror behind the sample and arrive beam splitter.Reference beam arrives beam splitter after entering the completely reflecting mirror of spending with inclined light shaft 45, reference beam is combined in and interferes with each other at beam splitter 9 with target beam, and as shown in Figure 2, digital hologram is collected by digital sensor (CMOS or CCD), hologram image produces, through the intelligent chip analyzing and processing.System adopts USB interface as upper interface, make things convenient for the user directly in real time data presentation on display screen, or directly link to each other and carry out the data storage record with computing machine.Simultaneously, system also is provided with wireless module, is convenient to the user and sends data in real time to remote control center.Wherein, wireless communication module can adopt widely used bluetooth module design, and further, wireless communication module also can adopt the gsm module design;
The signal intelligent analytical equipment of present embodiment comprises cmos image System on Chip/SoC and intellectual analysis chip.
Further, the input end of the output terminal of CMOS chip and intelligent chip links to each other;
Further, the output terminal of intelligent chip links to each other by USB interface with the input end of display screen;
Further, the output terminal of intelligent chip links to each other with the wireless transport module input end;
The LASER Light Source control device of present embodiment is placed on the fixed position in the housing, links to each other with laser generator.
Present embodiment also comprises the affixed of being connected of housing, base, power supply, reference beam passage and housing, light source generating means and inner shell, and control circuit for light source is connected with housing and lower device.
According to described embodiment, can carry out imaging to biological sample, and need not any preparation process, also need not sample is shifted out from the microbe culture container, the sample that contains microorganism is placed in the bottle of cultivation, and microwell plate is in ware or the pipe.
In addition, the utility model device also can be realized the fast detecting of pair cell, and analysis can be carried out under the condition that sample is not shifted out from cell culture container.Avoided interference to sample.Wherein, can also count to breeding in the container with dead cell or microorganism.
All optical elements all are arranged in the black salable cylindrical shell, thereby reduce the influence of extraneous parasitic light, have improved the precision that detects.

Claims (7)

1. portable wireless transmission image display type microbial rapid detection searchlight, comprise: light source takes place and control device, in the light path of light source rear end optical filter is installed, beam expanding lens, beam splitter with inclined light shaft 45 degree, objective table, completely reflecting mirror with inclined light shaft 45 degree, beam splitter, the signal intelligent analytical equipment, wireless transport module and display screen, it is characterized in that: described beam expanding lens front end is placed with optical filter, the beam splitter front end is placed with beam expanding lens, target beam through the beam expanding lens beam splitter shines the sample that objective table (7) is located to place, enter completely reflecting mirror (8) and arrive beam splitter, another reference beam through beam splitter enters into another completely reflecting mirror (10), locate to interfere at beam splitter (9) with target beam, image in the intellectual analysis device; Described signal intelligent analytical equipment comprises sensor assembly and intellectual analysis chip, the output terminal of described sensor assembly links to each other with the intelligent chip input end, the output terminal of intelligent chip is connected with input end and computer with display screen or by USB interface, and the input end of described wireless transport module links to each other with the output terminal of intelligent chip.
2. portable wireless transmission image display type microbial rapid detection searchlight according to claim 1 is characterized in that, described light source takes place and control device comprises laser generator and control circuit thereof.
3. portable wireless transmission image display type microbial rapid detection searchlight according to claim 1 is characterized in that described light source control device comprises collimating mirror, beam splitter, photodiode and control circuit thereof.
4. portable wireless transmission image display type microbial rapid detection searchlight according to claim 1 and 2 is characterized in that the light source of described light source generating means is the combination of one or more laser diode laser instruments.
5. portable wireless transmission image display type microbial rapid detection searchlight according to claim 1 is characterized in that described optical filter (4) is placed between beam splitter (3) and the beam expanding lens (5).
6. portable wireless transmission image display type microbial rapid detection searchlight according to claim 1 is characterized in that described target beam and reference beam are respectively through a completely reflecting mirror.
7. portable wireless transmission image display type microbial rapid detection searchlight according to claim 1, it is characterized in that, also comprise the affixed of being connected of housing, base, power supply, reference beam passage and housing, light source generating means and inner shell, control circuit for light source is connected with housing and lower device.
CN2009203511821U 2009-12-24 2009-12-24 Portable wireless transmission and image display type microorganism fast detection searchlight Expired - Fee Related CN201681045U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103773901A (en) * 2014-01-23 2014-05-07 程涛 Remote food bacteria detection control method and system based on microscopic vision
WO2014086323A1 (en) * 2012-12-07 2014-06-12 Axure Technologies S. A. Automated lamp with ultraviolet light for characterising rocks
CN112961768A (en) * 2021-02-04 2021-06-15 海南微氪生物科技股份有限公司 Leeuwenhoek real-time selective microorganism rapid detection system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014086323A1 (en) * 2012-12-07 2014-06-12 Axure Technologies S. A. Automated lamp with ultraviolet light for characterising rocks
CN103773901A (en) * 2014-01-23 2014-05-07 程涛 Remote food bacteria detection control method and system based on microscopic vision
CN112961768A (en) * 2021-02-04 2021-06-15 海南微氪生物科技股份有限公司 Leeuwenhoek real-time selective microorganism rapid detection system

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20101222

Termination date: 20141224

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