CN104568859B - Have fluorescence observation device, pedestal and the fluorescence microscope of multigroup different angle light source - Google Patents

Abstract

A kind of fluorescence observation device, pedestal and fluorescence microscope, wherein fluorescence observation device includes cover body, multi-angle luminescence unit and control unit, and cover body includes printing opacity heat insulation layer and heat dissipating layer, and there are multi-angle luminescence unit multiple narrow light fields to excite optical element, optical element heat conduction can be excited to be arranged on heat dissipating layer narrow light field for radiating, narrow light field with directive property excites the optical element Shu Ze that lights to pass through printing opacity heat insulation layer, and optical element is excited by the different narrow light field of control unit control multi-group light-emitting angle, to select irradiating angle, fluorescence observation device can be combined into pedestal with a pedestal, narrow light field excites optical element also can be on pedestal, use selective exitation irradiation angle, build up the environment needed for fluorescence observation and record.

Description

Have fluorescence observation device, pedestal and the fluorescence microscope of multigroup different angle light source

【Technical field】

The present invention is that, on a kind of fluorescence observation device, especially a kind of fluorescence available for fluorescence microscopy Optical devices is seen Survey device, fluorescence microscopy Optical devices pedestal and the device.

【Background technology】

Fluorescent technique further extends to biological study in recent years in addition to for industrial detection, counterfeit banknote identification and criminal identification In genetic engineering and gene turn plant etc. experimental analysis with follow the trail of, make fluoroscopic image differentiate with analysis importance gradually lifted.

So-called fluorescence, is mainly by the light irradiation of a high frequency on the thing to be observed with fluorescent characteristic, for example herein The anti-counterfeiting line of banknote, or the doubtful bloodstain in punishment case scene position, use and inspire the fluorescence compared with low frequency, along with appropriate Filter combination, just clearly can watch or capture the fluoroscopic image of above-mentioned banknote or bloodstain；As for being permitted for bio-science field Many researchs are to turn to plant for gene, and for ease of observation, the gene of implantation can often produce fluorescin, by the glimmering of thing to be observed Light reaction whether there is, and confirms the success or failure of gene insertion, and can be to turning to plant successfully biological further further investigation.

Fluorescence animal common at present includes white mouse, maggot, a variety of fish, jellyfish etc., because no matter big various experimental animals are Small size, or the excitation wavelength for occurring fluorescence is stimulated, difference is all very big each other.For build it is slightly larger, can be with naked-eye observation Animal, how the more difficult limitation of usual scope of activities limits to its scope of activities, and provides the appropriate auxiliary of economic and convenient operation Equipment is helped to observe, will be the homework that must be previously-completed before testing.If even experiment white mouse is fed in animal usually During center, because the reproductive capacity and energy of white mouse are all very strong, since being claimed experiment white mouse, it is necessary to differentiate and confirm that its is certain With fluorescence reaction, with greater need for the light fluorescence observation device being easy to carry about with one.

Have a kind of fluorogene zebra fish by numerous studies in recent years, be primarily due to such zebra fish breeding amount it is big and Breeding cycle is short, can produce a large amount of offsprings, and with the characteristic such as subhuman tract, or even expanded grinds to disease Study carefully, drug screening, poisonous substance test etc. correlative study.If however, experimental animal be such volume only as sesame size zebra fish, Then need to different multiplying microscope observed and recorded.The fluorescence microscope commonly used at present, although fluorescence microscope, which has, to be used On convenience, can effectively assist determinand of such as researcher observation with fluorescence reaction；However, due to fluorescence microscopy The manufacturer of mirror is generally the manufacturing overlord of original optical facilities, therefore the traveling road for focusing on how to constrain excitation beam more Line, in the hope of the error for increasing resolution in theory, reducing image, its framework does not depart from conventional microscopical major optical and set Meter.

However, when excitation beam is passed through apart from long, energy spreads with square distance inverse ratio to be weakened.Supported to solve exciting light During up to determinand, the problem of energy is not enough, existing fluorescence microscope greatly improves light-source brightness, causes part small such as spot The determinand of horse fish during observation, or even because it is heated excessively and caused by protein denaturation.Further, since exciting light is with vertical Direction forward direction irradiation thing surface to be observed, direct reflected light is very strong, and by absorbing the fluorescence that exciting light just discharges, intensity is far below Direct reflected light.That is, as the shorter intensity of reflected light of noise, wavelength, than really wanting observed and recorded, longer wavelengths of glimmering Light signal is also strong thousands of times, and signal noise (S/N) than too poor causes that extremely complex expensive optical texture need to be configured, and also causes Fluorescence microscope millions of valuation easily.

Especially existing fluorescence microscope is incompatible with general light microscope, also in a disguised form forces requirement observation fluorescence reaction person Existing microscope in research department must be abandoned, the high fluorescence microscope of price is bought again, and does not meet general expectation, especially Its microscope price and its performance correlation are very high, and the quality of simple eyeglass number and manufacturing process is just enough significantly to influence it Price, if having purchased high performance general light microscope, unlikely another purchase one is same high performance glimmering easily Light microscope.Therefore, how by additional accessory, general light microscope is allowed also to be transformed into lockable fluorescent characteristic easily Fluorescence microscope, the emphasis also further studied as the present invention.

For ease of analyzing and observing, the related Bioexperiment of current fluorescence, usually use by ultraviolet light, dark blue light or Green glow excites and discharged the fluorescin of blue and green light or feux rouges.A kind of existing gram of difficult servicing unit 70 is as shown in Figure 10, is Ultraviolet source 72 directly is set with such as two front ends of snake belly tube 74, and allows user voluntarily to curve snake belly tube 74 and captures appropriate light Irradiation angle, exposes to the culture dish 76 for being loaded with fluorescence thing to be seen, uses generation luminescence generated by light, but be limited to snake belly tube 74 Structure, when the object lens 78 and object of light microscope are apart from only two or three centimeter, snake belly tube 74 will be difficult to from so narrow and small Space transfer fold into.Not only angle can not precision adjust so that lighting angle is often not as expected, and in illumination range Brightness is not easy to uniformly so that the image of acquisition can not be analyzed by accurate quantification.

And the experimental animal such as zebra fish is still live body in an experiment, so when carrying out above-mentioned fluorescence microscopy experiment, when treating Observation thing is such as fluorescence zebra fish or maggot, object may constantly shift position, in addition spot movable in small water droplet Horse fish is also possible to dead in an experiment because of dissolved oxygen deficiency in water droplet, it is necessary to while considering operating time and experimental situation to biology Influence, uneven small range illumination is even more to make us being fed up with, if operate unskilled or bad luck, be also easy for be oriented to Abortive experiment result.Relatively large animal such as white mouse etc., can not more order it and obediently stay in light source range of exposures, and it swims everywhere Walk to gnaw, the damage and pollution of experiment equipment are will also result in sometimes.

In order to be able to enable above-mentioned living body biological to obtain good lighting effect, present invention applicant is first the 201210402931.5 application cases " microoptic capture device fluorescence accessory module and the microoptic capture device ", such as scheme It is to be coated the predetermined observation position of pedestal 84 wherein with shade 82, and one can be surrounded out jointly with pedestal 84 shown in 11 Light covers chamber 86, is arranged on light masking chamber 86 for thing to be observed, for observation, and is provided with the internal face of shade 82 many Individual light source assembly 88, and light source assembly 88 is finely tuned along a track by regulating part 80, in the movement of the surface of shade 82, use More preferably light angle is provided.

Although above-mentioned direction can cause the convenience in uniform illumination, increase observation, if the heat produced by light source assembly It is without good hot channel so that when heat is set to stay in light containment chamber room, it would still be possible to cause temperature to raise, causes final Experimental result it is not good, and in order to obtain optimal light angle, it is necessary to the adjustment structure of design of mechanical or electronic type in addition, Development cost is difficult to reduce, and for a long time using being also easily damaged, need to carry out additional maintenance maintenance, therefore still have room for improvement.

Therefore, selection, integrated operation can be adjusted easily from a kind of uniform illumination, lighting angle facilitate rapid fluorescence Observation device, allows researcher during observed and recorded, and light source, and appropriate covering outer interference are operated without constantly diverting one's attention Light, allows experimentation smoothly and is easy to observed and recorded, while increasing radiating effect, the heat produced by making illumination is difficult to be trapped in In shade, slow down temperature rising, and simplify the structure of lighting angle regulation, reduce production cost, lift product service life.

In summary, the present invention provides a kind of more excellent fluorescence observation device and microoptic capture device, on the one hand, When experimental animal is larger, the fluorescence observation device of simple type can be selected, is directly observed and is recorded.Relatively, in reality Test animal volume it is smaller when, can also coordinate exquisiter fluorescence observation device or be bound to the fluorescence observation device microscopical In pedestal, being easily promoted to general microscope or microcamera can be in response to fluorescence Research Requirements；Another aspect is simplifying knot While structure, lifting operation convenience, the quality of microexamination record will not be also sacrificed, and propose a highly stable and letter Clean operating platform, meets miscellaneous experimental applications requirement, while having the advantage of price extremely economy, it is possessed nothing Analogous cost performance.

The present invention is totally different from conventional fluorescence microscope design, changes with proximity light-source structure, angle may be selected in design Proximity excitation source, and details in a play not acted out on stage, but told through dialogues fluoroscopic image can be obtained.And by dark-ground illumination, exclude reflected light interference so that incident intensity Degree can significantly weaken, and still can ensure that image quality；Another aspect utilizes proximity light source, and light-source brightness is greatly reduced once again so that LED is smoothly imported, and is arranged on thing periphery to be observed, shortens the approach that incident light is passed through, especially with one layer of printing opacity and Adiabatic internal layer shell, it is to avoid unnecessarily rise is observed the temperature in region, also make it that the larger animal that is observed will not Contact or be stained light source.Simplify structure design, manufacturing cost is greatly reduced, even can also coordinate existing separately as accessory There is light microscope to operate with.Via the announcement of the present invention, allow research staff can extremely economic price, obtain with it is existing The compatible product of equipment, greatly improves research quality.

【The content of the invention】

It is an object of the present invention to provide a kind of fluorescence observation device of modular structure so that the behaviour of research observation Work simplifies, and forms the success rate of a stabilization and the experiment of friendly operating platform, further Study of Lifting.

Another object of the present invention is to provide a kind of fluorescence observation device that heat is produced for isolation light source assembly, it is to avoid by The biological living of detection directly touches light source assembly, or is scalded or burned tested dynamic because heat energy passes into observing environment Thing.

It is still another object of the present invention to provide a kind of with the glimmering of printing opacity thermal insulation layer isolation experimental animal contact light source assembly Light observation device, it is to avoid experimental animal damages, pollution proximity light source.

A further object of the present invention is to provide a kind of pedestal with fluorescence observation device, because low angle therein swashs Light emitting source has the light-emitting component of multigroup different lighting angle, and structure simplifies, and can adjust the fluorescence observation device of light emission direction, drop Low production cost and lifting product service life.

A further object of the present invention is to provide a kind of pedestal with above-mentioned fluorescence observation device, due to it is existing typically The microoptic capture device such as light microscope or microcamera has high compatibility, therefore can link easily, is lifted existing Light microscope increases the application elasticity of general microoptic capture device to fluorescence purposes.

The another object again of the present invention is to provide a kind of pedestal of the fluorescence microscopy Optical devices with shade, can hidden Cover the interference of extraneous strong light and veiling glare, the quality of lifting fluorescence microscopy imaging.

A further object again of the present invention is to provide a kind of fluorescence microscope with above-mentioned fluorescence observation device, by carrying For proximity light source, luminous intensity is reduced, adjoint meaningless heat energy is reduced, and the heat produced by making light source will not meaningless entrance shade It is interior, it is further added by the radiating effect of shade so that the temperature of thing local environment to be observed is difficult rapid rising.

The more purpose again of the present invention is to provide a kind of fluorescence microscope for having radiating effect, light source is changed in energy When the heat energy that gives out by diversion, interference when reduction temperature rises to thing to be observed lifts the efficiency and precision of experiment.

A kind of fluorescence observation device of the multigroup different angle light source of tool of the present invention, is to be placed on a carrying observation area for cover, For one fluorescence thing to be observed positioned at carrying observation area of observation, the wherein fluorescence observation device includes：One cover body, it is and above-mentioned Carrying observation area surrounds out an accommodation space for accommodating fluorescence thing to be observed jointly, and the cover body is formed with an observation Hole, the cover body includes：One layer of printing opacity heat insulation layer, is set towards the accommodation space direction；And one layer of heat dissipating layer, its thermal conductivity coefficient Higher than above-mentioned printing opacity heat insulation layer and be at least less than above-mentioned printing opacity heat insulation layer in the light transmittance of visible-range, the heat dissipating layer be with One side is arranged on the direction of the printing opacity heat insulation layer away from the accommodation space；The fluorescence observation of the above-mentioned multigroup different angle light source of tool Device also includes one group of multi-angle luminescence unit, and optical element, each foregoing narrow light field exciting light member are excited with multiple narrow light fields The luminous beam of part institute is passed through above-mentioned printing opacity heat insulation layer respectively, exposes to the accommodation space, and foregoing narrow light field excites optical element at least With multiple different irradiating angles each other；And a control unit, it is for controlling at least the above of the multi-angle luminescence unit Narrow light field excites the irradiating angle of optical element.

A kind of pedestal of fluorescence microscopy Optical devices of the present invention, is for one fluorescence thing to be observed of observation, the pedestal Including：One pedestal, the carrying observation area with one for observation fluorescence thing to be observed；One cover body, it is common with said base An accommodation space for accommodating fluorescence thing to be observed is formed with together, and the cover body is formed with a peep hole, the cover body bag Include：One layer of printing opacity heat insulation layer, is set towards the accommodation space direction；And one layer of heat dissipating layer, its thermal conductivity coefficient is higher than above-mentioned Light heat insulation layer and at least visible-range light transmittance be less than above-mentioned printing opacity heat insulation layer, the heat dissipating layer is set with a side Put in the direction of the printing opacity heat insulation layer away from the accommodation space；The pedestal also includes one group of multi-angle luminescence unit, with multiple Narrow light field excites optical element, and each foregoing narrow light field excites the luminous beam of optical element institute to pass through respectively above-mentioned printing opacity heat insulation layer, irradiation To the accommodation space, and foregoing narrow light field excites optical element at least to have multiple different irradiating angles each other；And a control Unit, is for controlling at least the above narrow light field of the multi-angle luminescence unit to excite the irradiating angle of optical element.

A kind of fluorescence microscopy Optical devices of the present invention, are to supply one fluorescence thing to be observed of observation, including：One fuselage； One be installed in the fuselage object lens；And a fluorescence observation device, including：One pedestal, with one for observing the fluorescence The carrying observation area of thing to be observed；One cover body, one is collectively forming for accommodating fluorescence thing to be observed with said base Accommodation space, and the cover body are formed with a peep hole, and treating positioned at the accommodation space is captured through the peep hole for the object lens Thing image is observed, the cover body includes：One layer of printing opacity heat insulation layer, is set towards the accommodation space direction；And one layer of heat dissipating layer, its Thermal conductivity coefficient is less than above-mentioned printing opacity heat insulation layer higher than above-mentioned printing opacity heat insulation layer and at least in the light transmittance of visible-range, and this dissipates Thermosphere is to be arranged on the direction of the printing opacity heat insulation layer away from the accommodation space with a side；The fluorescence observation device also includes one Group multi-angle luminescence unit, optical element is excited with multiple narrow light fields, and each foregoing narrow light field excites the luminous beam point of optical element institute Do not pass through above-mentioned printing opacity heat insulation layer, expose to the accommodation space, and foregoing narrow light field excite optical element at least have it is multiple each other Different irradiating angle；And a control unit, it is at least the above narrow light field exciting light for controlling the multi-angle luminescence unit The irradiating angle of element.

Therefore, fluorescence observation device of the invention, fluorescence microscopy Optical devices pedestal and the device, are to utilize cover body Arc curve so that the narrow light field being arranged on cover body or pedestal excites optical element to have multiple different light emitting angers respectively Degree, the narrow light field that can pass through control unit control diverse location excites optical element, to obtain required lighting angle；Also it is optional Select and excite optical element to be arranged on pedestal narrow light field, also with the arc curve of cover body so that positioned at the narrow of diverse location Light field excites optical element issued light beam, and different angles can be reflexed to by cover body, also can be another in pedestal when being arranged on pedestal One layer of heat-conducting layer is set so that narrow light field excites the heat produced by optical element, can be by the heat dissipating layer of cover body or the heat-conducting layer of pedestal Diversion makes the temperature in accommodation space be difficult rising, interference during reduction observation living body biological, and the structure of light regulation to outside Simply, production cost can be not only reduced, while can also lift product service life.

Another aspect, fluorescence observation device has the characteristic for simplifying operation, can be connected easily in known optical microscope or The microoptic capture device such as microcamera, increases the application of microoptic capture device, meets the expectation of consumer, The efficiency and precision of experiment are lifted, so as to obtain the micro- information of height of fluorescence thing to be observed, and reaches all above-mentioned purposes.

【Brief description of the drawings】

Fig. 1 is the side view of the first preferred embodiment of the fluorescence microscopy Optical devices of the present invention；

Fig. 2 is the Partial enlarged side view of Fig. 1 fluorescence microscopy Optical devices, is the structure for illustrating fluorescence observation device；

Fig. 3 is the block diagram of Fig. 1 fluorescence microscopy Optical devices, is the company for illustrating control unit and multi-angle luminescence unit Knot；

Fig. 4 is the top view that spreads apart of soft driving plate of Fig. 2 fluorescence observation device；

Fig. 5 is the side view that one group of water cooling tube is buried in the heat dissipating layer of Fig. 1 fluorescence microscopy Optical devices；

Fig. 6 is the side view of the second preferred embodiment of fluorescence observation device, and fluorescence observation device can individually be placed on desktop Use；

Fig. 7 is the side view of the preferred embodiment of the pedestal of fluorescence microscopy Optical devices, is to illustrate pedestal and cover body phase Mutually combination, and narrow light field excites optical element to be provided on pedestal；

Fig. 8 is the block diagram of Fig. 7 fluorescence microscopy Optical devices, is the company for illustrating control unit and multi-angle luminescence unit Knot；

Fig. 9 is the top view of Fig. 7 circuit board being arranged in pedestal；

Figure 10 is the side view of the servicing unit of known optical；And

Figure 11 is the side view of the auxiliary shade of known optical.

【Symbol description】

5th, 5 ' ... fluorescence thing 1 ... fuselage to be observed

2 ... object lens 3 ... fluorescence observation devices

32nd, the cover body of 32 " ... pedestal 34,34 ', 34 " ...

36th, 36 " ... multi-angle luminescence units 38,38 " ... control units

30 ... soft driving plate 322 ... carrying observation areas

340th, 340 ', 340 " ... accommodation spaces 342,342 ' ... peep holes

344th, 344 ' ... printing opacity heat insulation layers 346,346 " ... heat dissipating layers

362nd, 362 ', 362 " ... narrow light fields excite optical element 31 ... water cooling tube

324 " ... heat-conducting layer 360 " ... circuit boards

326 " ... thermal isolation photic zone 341 " ... reflectings surface

348 ' ... support lateral margin 341 ' ... filters

360 ... connect circuit board 344 " ... printing opacity reflecting layer

72 ... ultraviolet source 74 ... snake belly tubes

76 ... culture dish 78 ... object lens

80 ... regulating part 82 ... shades

84 ... pedestal 86 ... light cover chamber

88 ... light source assembly 70 ... servicing units

6 ' ... desktops

【Embodiment】

For the present invention foregoing and other technology contents, feature and effect, in the preferable of following cooperation Figure of description In the detailed description of embodiment, can clearly it present；Same or analogous element, by with similar label sign.

The fluorescence microscopy Optical devices of the present invention, are for one fluorescence thing to be observed of detection as shown in Figure 1, Figure 2 and Figure 3 5, mainly include fuselage 1, object lens 2 and fluorescence observation device 3, wherein object lens 2 are to install on the fuselage 1, and fluorescence observation device 3 include pedestal 32, cover body 34, multi-angle luminescence unit 36, control unit 38 and soft driving plate 30, and pedestal 32 is then tool There is a carrying observation area 322, and covered for cover body 34, cover body 34 has been collectively forming an accommodation space 340 with pedestal 32, And on cover body 34, more open up and be formed with a peep hole 342, it is available for the correspondence assembling of object lens 2.

Cover body 34 includes printing opacity heat insulation layer 344 and heat dissipating layer 346, and by printing opacity heat insulation layer 344 towards accommodation space 340 Direction set, in this example, printing opacity heat insulation layer 344 is to be illustrated as acrylic material, and heat dissipating layer 346 is then towards away from accommodating The direction in space 340, by the side in the inner side direction of heat dissipating layer 346, complete cladding printing opacity heat insulation layer 344, and the material of heat dissipating layer 346 Matter, then be the material for being higher than printing opacity heat insulation layer 344 from thermal conductivity coefficient, and at least in visible-range, its light transmittance is less than Printing opacity heat insulation layer 344, heat dissipating layer 346 is by taking metal material as an example in this example.

Multi-angle luminescence unit 36 includes multiple narrow light fields and excites optical element 362, and is installed in cover body 34, herein Defined narrow light field excites optical element 362, and it is to be limited in a predetermined spatial angle range to refer to its luminous light field, for example, go out Angular is that 360 degree of solid angles light within the solid angle of tens of degree, rather than to all the winds.In the lump with reference to shown in Fig. 4, It is multiple LED elements in this example that narrow light field, which excites optical element 362, each LED element be first welded on respectively one piece it is rigid Connect on circuit board 360, then connected by circuit board 360 is connected to soft driving plate 30, and soft driving plate 30 in this example Shape be then star-shaped extension, and thermal conductive contact makes foregoing narrow light field exciting light to the surface of heat dissipating layer 346 of cover body 34 The accommodation space 340 that beam is directed towards inside cover body 34 that lights of element 362 is sent, and printing opacity heat insulation layer 344 of passing through respectively.

Because the side of the cover body 34 of this example is that arc extension is presented, therefore it is installed in the narrow light field exciting light in cover body 34 Element 362 will be influenceed by structure so that the multiple narrow light fields for being arranged on different sides position excite optical element 362, can divide Different rising angle is not produced.And each narrow light field of this example excite optical element 362 can controlled unit 38 control, by The narrow light field of driving diverse location excites the mode of optical element 362, adjusts the irradiating angle that narrow light field excites optical element 362, for example Narrow light field this example being arranged in cover body 34 excites 362 points of optical element to be upper, middle and lower-ranking, and is controlled by control unit 38 The excitation source of optical element 362 is excited positioned at the narrow light field of different curved layers so that narrow light field excites the irradiating angle energy of optical element Controlled depending on demand.

Because the multi-angle luminescence unit 36 of the present invention is nearby lighting in thing to be observed, what such a proximity light source was passed through Light path is very short, and the loss of light is less, it is possible to the high brightness excitation beam for avoiding conventional fluorescence microscope from using, The high heat for avoiding prior art adjoint in the lump, allows light source used in the fluorescence microscope or fluorographic unit of the present invention to be unlikely to Thing to be observed is baked.

Another aspect, when fluorescence to be detected thing 5 to be observed, the carrying that fluorescence thing 5 to be observed first is placed in into pedestal 32 is seen Examine in area 322, and cover body 34 is covered in pedestal 32, fluorescence thing 5 to be observed is placed in accommodation space 340, and by controlling Unit 38 processed controls narrow light field to excite the excitation source of optical element 362, stimulates fluorescence thing 5 to be observed to produce fluorescence, saturating for observer Object lens 2 are crossed from the directly fluorescence reaction that sends of observation fluorescence thing 5 to be observed of peep hole 342, and acquisition observed it is to be observed Thing image.

As it was previously stated, when the present invention takes low angle to light, such as allowing lighted beam to be less than 45 degree with horizontal sextant angle, now There will not be the exciting light directly reflected by a large amount of incident return object lens 2, at most only a small amount of exciting light, which is diffusely reflected, to be constituted Noise interference, a lot of fluorescence message fainter than exciting light is also because signal noise is than being preferably easier to observed with record .Compared to existing fluorescence microscope, its optical design is in order to allow light beam to follow object lens direction direct irradiation to thing to be observed, it is impossible to Avoid the interference that exciting light directly reflects so that signal noise also forces rear end to want meaningless and install a large amount of optical treatments additional than extreme difference Or electronic processing device, therefore the present invention can enable detection data relatively sharp, and existing light microscope etc. of directly arranging in pairs or groups, It is substantially improved and uses elasticity.

The heat produced by optical element 362 is excited due to soft driving plate 30 and narrow light field, by directing contact to heat dissipating layer 346, the outer surface of cover body 34 can be rapidly transferred to, and diffuse in extraneous air, and as being with sub- gram in this example Masterpiece is printing opacity heat insulation layer 344, because the coefficient of heat conduction of acrylic is far below the heat dissipating layer 346 of metal material, is successfully avoided Temperature rise in accommodation space 340.Also fluorescence thing 5 to be observed is avoided to be produced because of the rise of environment temperature with regard to this bad anti- Should be even dead.Certainly, it is increase radiating effect, more can be as shown in figure 5, one group is buried in heat dissipating layer 346 be illustrated as water cooling The heat-sink unit of pipe 31, at least part of water cooling tube 31 is buried in heat dissipating layer 346, and provides current by continuous, makes heat dissipating layer 346 heat energy transmitted, the water being water cooled in pipe 31 is exported really, and the radiating effect of heat dissipating layer 346 is substantially improved.

When thing to be observed is interpreted as the larger animal of the builds such as fluorescence mouse such as above-mentioned example, fluorescence observation device of the invention is also Reality can be applied in way of further example, as shown in fig. 6, will now use the cover body 34 ' of the larger such as mouse cage of volume, and be single Solely use, fluorescence thing 5 ' to be observed is placed directly on a desktop 6 ', and the cover of cover body 34 ' is placed on desktop 6 ' and forms accommodating Space 340 ', and fluorescence thing 5 ' to be observed is accommodated wherein, then drive narrow light field to excite optical element 362 ' to light by testing staff, Whether fluorescence thing 5 ' to be observed has fluorescence reaction directly from peep hole 342 ', and when fluorescence thing 5 ' to be observed is to be illustrated as During fluorescence mouse, because fluorescence mouse can arbitrarily be moved in accommodation space 340 ', and the printing opacity heat insulation layer 344 ' of cover body 34 ' can be provided Thermal insulation protection, it is to avoid fluorescence mouse directly touches narrow light field and excites optical element 362 ' and scald, and also fluorescence mouse can be avoided directly to gnaw Sting light source or manure contamination equipment.

Another aspect, the peep hole 342 ' of this example more protrudes out one section of support lateral margin towards the lateral edge locations of accommodation space 340 ' 348 ', and it is another a piece of filter 341 ' is arranged on the position of peep hole 342 ', and be supported the support of lateral margin 348 ', and filter 341 ' setting, is that the wavelength light that completely cut off the non-predetermined fluorescence such as exciting light is penetrated, so that testing staff observes fluorescence Reaction.

Certainly, fluorescence observation device of the invention, which can be more mounted directly, is built into one group of microscope pedestal, and by making User installs existing general light microscope body additional on pedestal, you can the fluorescence microscope of fluorescence can be observed by arranging in pairs or groups out. The preferred embodiment of pedestal of the present invention, as shown in Figures 7 and 8, in this example, pedestal is by pedestal 32 " and the mutually group of cover body 34 " Into wherein pedestal 32 " includes thermal isolation photic zone 326 " and heat-conducting layer 324 ", and wherein thermal isolation photic zone 326 " equally can be with Acrylic material is made, and heat-conducting layer 324 " is then that side is arranged on into thermal isolation photic zone 326 " and away from accommodation space 340 " Direction, and heat-conducting layer 324 " can also metal material be made so that the thermal conductivity coefficient of heat-conducting layer 324 " be higher than thermal isolation printing opacity Layer 326 ".

The multi-angle luminescence unit 36 " of this example has further included a piece of circuit board 360 ", and is set in the way of thermal conductive contact In on heat-conducting layer 324 ", optical element 362 " is excited to connect setting for narrow light field so that narrow light field excites optical element 362 " to light Beam is passed through thermal isolation photic zone 326 " and towards the excitation source of accommodation space 340 " respectively, wherein narrow light field excites optical element 362 " And the heat produced by circuit board 360 ", then outside can be directed to by heat-conducting layer 324 ".

In the lump with reference to shown in Fig. 9, the narrow light field of this example excite optical element 362 " be distribution be arranged in pedestal 32 ", in, Outer three different ring positions, and control the narrow light field respectively positioned at diverse location to excite optical element by control unit 38 " 362 ", and the heat dissipating layer 346 " of cover body 34 " is towards the side in printing opacity reflecting layer 344 ", then is that a light is formed through polishing Sliding minute surface is as reflecting surface 341 ", when narrow light field excites the luminous beam of the institute of optical element 362 " to reflecting surface 341 ", due to cover body 34 " side is that arc extension is presented so that the reflection angle of different incident beams is different each other, therefore, can pass through control single Narrow light field of the control of member 38 " positioned at different ring positions excites the excitation source of optical element 362 ", to obtain required light source.Especially In this example, even can also be different color light respectively with the LED on Radius, the LED of such as outer ring be ultraviolet respectively Light and green glow are interconnected, during with response to experiment, while there is the thing to be observed of two kinds of fluorogenes.

In this example, six LEDs of innermost circle are then white light LEDs, the normal visible illumination of offer are used, so as to operator It will be clear that observation for example has a few tail zebra fish in culture dish on earth, certainly, it is readily appreciated to be such as familiar with the art personage, Narrow light field described in various embodiments of the present invention excites the number or allocation position of optical element, be all for ease of illustration for the sake of, not Limit the implementation of the present invention.

In summary, fluorescence observation device of the invention, fluorescence microscopy Optical devices pedestal and the device, can pass through control The narrow light field of unit control diverse location processed excites optical element excitation source so that be arranged on the narrow light field exciting light member on cover body Part sends the light source of different angles, or excites optical element to be arranged on pedestal narrow light field, reflexes to light source not through cover body Same angle, is simplified the structure that light is adjusted, production cost can be not only reduced, while can also lift product service life.

And narrow light field excites the heat produced by optical element, soft driving plate and circuit board, can all pass through the heat dissipating layer of cover body Or the heat-conducting layer of pedestal by thermal conductivity to outside so that the temperature in accommodation space can make living body biological be difficult by stability contorting Disturbed by temperature change, increase the efficiency and precision of experiment, when fluorescence thing to be observed is to be illustrated as fluorescence mouse, printing opacity Heat insulation layer can then be used as thermal insulation protection, it is to avoid fluorescence mouse is scalded because directly touching narrow light field excites optical element, causes fluorescence The physiological reaction of mouse, which is affected or polluted, damages light source.

It is most important, due to fluorescence observation device provide near field light source, exciting light transmitting procedure loss reduce, also because It is, with dark-ground illumination mode operation, further to exclude directly reflect a large amount of and excite optical noise, not only simplify light for exciting light Source is designed, and signal noise ratio is improved by a larger margin so that fluorescence observation device of the invention can be used alone, also can be easily It is connected in the microoptic capture device such as known optical microscope or microcamera, increases the application of microoptic capture device Scope, meets the expectation of consumer, and reaches above-mentioned all purposes.

Only as described above, only presently preferred embodiments of the present invention, it is impossible to which the scope that the present invention is implemented is limited with this, Every simple equivalent changes and modifications made according to claims of the present invention and description, all should still belong to of the invention special In the range of profit covers.

Claims (8)

1. a kind of fluorescence observation device for having multigroup different angle light source, is to be placed on a carrying observation area for cover, for observation one Fluorescence thing to be observed positioned at carrying observation area, wherein fluorescence observation device includes：

One cover body, an accommodation space for accommodating fluorescence thing to be observed is surrounded out with above-mentioned carrying observation area jointly, and The cover body is formed with a peep hole, and the cover body includes：

One layer of printing opacity heat insulation layer, is set towards the accommodation space direction；And

One layer of heat dissipating layer, its thermal conductivity coefficient higher than above-mentioned printing opacity heat insulation layer and at least visible-range light transmittance less than upper Printing opacity heat insulation layer is stated, the heat dissipating layer is to be arranged on the direction of the printing opacity heat insulation layer away from the accommodation space with a side；

One group of multi-angle luminescence unit, optical element is excited with multiple narrow light fields, and each foregoing narrow light field excites optical element to be sent out Light beam is passed through above-mentioned printing opacity heat insulation layer respectively, exposes to the accommodation space, and to excite optical element at least to have more for foregoing narrow light field Individual different irradiating angle each other；And

One control unit, is for controlling at least the above narrow light field of the multi-angle luminescence unit to excite the illumination angle of optical element Degree.

2. fluorescence observation device as claimed in claim 1, further includes a pedestal, foregoing pedestal has the carrying observation area.

3. fluorescence observation device as claimed in claim 2, wherein the multi-angle luminescence unit are provided on the pedestal, and The heat dissipating layer towards this of the printing opacity heat insulation layer be a reflecting surface, the exciting light sent out for above-mentioned multi-angle luminescence unit sideways Reflected illumination is to the accommodation space；Wherein the pedestal includes：One layer of thermal isolation photic zone, is set towards the accommodation space direction Put；And one layer of heat-conducting layer, its thermal conductivity coefficient is higher than above-mentioned thermal isolation photic zone, and is arranged on the thermal isolation printing opacity with a side Direction of the layer away from the accommodation space；And above-mentioned multi-angle luminescence unit further includes at least a piece of circuit board, and aforementioned circuit Plate is that thermal conductive contact is arranged at the heat-conducting layer.

4. fluorescence observation device as claimed in claim 1, further includes at least a piece of soft driving plate, and foregoing soft driving plate It is the above-mentioned heat dissipating layer of the thermal conductive contact cover body, excites optical element to be arranged in foregoing soft driving plate for above-mentioned narrow light field.

5. fluorescence observation device as claimed in claim 4, further include an at least part and bury radiating list in the heat dissipating layer Member.

6. a kind of pedestal of fluorescence microscopy Optical devices, is that, for one fluorescence thing to be observed of observation, the pedestal includes：

One pedestal, the carrying observation area with one for observation fluorescence thing to be observed；

One cover body, an accommodation space for accommodating fluorescence thing to be observed, and the cover body are collectively forming with said base A peep hole is formed with, the cover body includes：

One layer of printing opacity heat insulation layer, is set towards the accommodation space direction；And

One layer of heat dissipating layer, its thermal conductivity coefficient higher than above-mentioned printing opacity heat insulation layer and at least visible-range light transmittance less than upper Printing opacity heat insulation layer is stated, the heat dissipating layer is to be arranged on the direction of the printing opacity heat insulation layer away from the accommodation space with a side；

One group of multi-angle luminescence unit, optical element is excited with multiple narrow light fields, and each foregoing narrow light field excites optical element to be sent out Light beam is passed through above-mentioned printing opacity heat insulation layer respectively, exposes to the accommodation space, and to excite optical element at least to have more for foregoing narrow light field Individual different irradiating angle each other；And

One control unit, is for controlling at least the above narrow light field of the multi-angle luminescence unit to excite the illumination angle of optical element Degree.

7. pedestal as claimed in claim 6, further includes at least a piece of soft driving plate and a heat-sink unit, and foregoing soft The above-mentioned heat dissipating layer of the driving plate system thermal conductive contact cover body, excites optical element to be arranged on foregoing soft driving plate for above-mentioned narrow light field On, the heat-sink unit is at least partly to bury the water cooling tube in the heat dissipating layer.

8. a kind of fluorescence microscopy Optical devices, are to supply one fluorescence thing to be observed of observation, including：

One fuselage；

One be installed in the fuselage object lens；And

One fluorescence observation device, including：

One pedestal, the carrying observation area with one for observation fluorescence thing to be observed；

One cover body, an accommodation space for accommodating fluorescence thing to be observed, and the cover body are collectively forming with said base A peep hole is formed with, the thing image to be observed for being located at the accommodation space, the cover body is captured through the peep hole for the object lens Including：

One layer of printing opacity heat insulation layer, is set towards the accommodation space direction；And

One layer of heat dissipating layer, its thermal conductivity coefficient higher than above-mentioned printing opacity heat insulation layer and at least visible-range light transmittance less than upper Printing opacity heat insulation layer is stated, the heat dissipating layer is to be arranged on the direction of the printing opacity heat insulation layer away from the accommodation space with a side；

One group of multi-angle luminescence unit, optical element is excited with multiple narrow light fields, and each foregoing narrow light field excites optical element to be sent out Light beam is passed through above-mentioned printing opacity heat insulation layer respectively, exposes to the accommodation space, and to excite optical element at least to have more for foregoing narrow light field Individual different irradiating angle each other；And

One control unit, is for controlling at least the above narrow light field of the multi-angle luminescence unit to excite the illumination angle of optical element Degree.