CN111269815A - Bacteria calculation image acquisition device - Google Patents
Bacteria calculation image acquisition device Download PDFInfo
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- CN111269815A CN111269815A CN202010092062.5A CN202010092062A CN111269815A CN 111269815 A CN111269815 A CN 111269815A CN 202010092062 A CN202010092062 A CN 202010092062A CN 111269815 A CN111269815 A CN 111269815A
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- inner cylinder
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- bacteria
- image acquisition
- calculation image
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The utility model belongs to the technical field of the colony count technique and specifically relates to a bacteria calculation image acquisition device is related to, bacteria calculation image acquisition device includes: a projection member and a sealing member connected in sequence; the sealing component comprises a first shell and a first inner cylinder, and the first shell can rotate relative to the first inner cylinder; the first shell is provided with a first opening part, the first inner cylinder is provided with a second opening part, and when the first inner cylinder rotates to a preset position, the first opening part and the second opening part are overlapped and used for placing the fungus box in the first inner cylinder; when the first inner cylinder deviates from a preset position, a closed space is formed inside the first inner cylinder. The application provides a bacterium calculates image acquisition device avoids light to run off through placing the fungus box in inclosed space, ensures illumination intensity for light shines and can clearly show to the image of colony formation, avoids the image bacterial colony that becomes to lose, therefore ensures the accuracy of the result that the later stage was counted the bacterial colony.
Description
Technical Field
The application relates to the technical field of bacterial colony counting, in particular to a bacteria calculation image acquisition device.
Background
At present, the bacterial counting is mainly a manual counting mode, which wastes time and labor and is easy to make mistakes. Consequently, there is the team to begin to study automatic colony counting technique, and colony image acquisition is the basis of realizing the colony count, transmit the result of gathering to the computer through image acquisition and analyze and curve, can calculate the order of magnitude that the quantity of target colony corresponds, realize bacterium automatic counting, but light is great to the definition influence of image, current colony image acquisition device seal is not good, shine to the light of fungus box and diverge easily, the loss, it is not concentrated, some colonies probably receive the light influence and can't clearly show in the image, consequently, the phenomenon that the colony lost probably causes, influence the later stage and carry out the accuracy of the result of counting to the colony.
Disclosure of Invention
The utility model provides a purpose provides a bacteria calculation image acquisition device to it is not good to solve the colony image acquisition device leakproofness that exists among the prior art to a certain extent, shines to the light of fungus box and runs off easily, the technical problem that intensity is not enough.
The application provides a bacteria calculation image acquisition device, which comprises a projection component for imaging and a sealing component connected with the projection component;
the sealing component comprises a first outer shell and a first inner cylinder, and the first outer shell can rotate relative to the first inner cylinder;
the first shell is provided with a first opening part, the first inner cylinder is provided with a second opening part, and when the first inner cylinder rotates to a preset position, the first opening part and the second opening part are overlapped and used for placing the fungus box in the first inner cylinder;
when the first inner cylinder deviates from the preset position, a closed space is formed inside the first inner cylinder.
In the above technical solution, further, the first housing has a cylindrical structure with one end open, and the one end open of the first housing is formed with an insertion part.
In any of the above technical solutions, the first inner cylinder has a cylindrical structure, one end of the first inner cylinder is provided with a screw ring, the screw ring protrudes relative to a surface of the first inner cylinder, and the screw ring can cover the insertion part in a state where the first inner cylinder is embedded in the first housing.
In any one of the above technical solutions, further, the lamp box further includes a lamp box base, the lamp box base includes a third housing and a base, the third housing is detachably connected to the second end of the first housing, and the base is provided with a light source.
In any one of the above technical solutions, the third housing has a tubular structure with an opening at one end, a stage is disposed at a bottom of the third housing opposite to the opening, the stage is located inside the first inner tube in a state where the first housing is assembled to the third housing, and a receiving groove is formed in the stage for receiving the fungi box.
In any one of the above technical solutions, further, light trap and installation department have been seted up to the bottom of third shell, the installation department is used for fixed filter, the light trap is used for making the process the filterable light of filter gets into the inside of first inner tube and shine to the fungus box.
In any one of the above technical solutions, further, the objective table is provided with a clamp for pressing in the center of the heart, and the clamp for pressing in the center of the heart is used for fixing the fungus box in the accommodating groove.
In any one of the above technical solutions, further, the stage is provided with a camera, and a lens of the camera faces the projection member.
In any one of the above technical solutions, the projection member further includes a second inner cylinder and a second outer shell, the second outer shell is detachably connected to the first end of the first outer shell, the second inner cylinder is connected to the second outer shell by a screw, and the second inner cylinder is provided with a projection screen.
In any of the above technical solutions, a storage portion is further formed between the third housing and the base for storing a power supply.
Compared with the prior art, the beneficial effect of this application is:
the application provides a bacteria calculation image acquisition device includes: the bacteria box is placed in the sealing component, the light source can irradiate the bacteria box to form an image, the formed image can be projected to the projection component, and the subsequent colony counting can be carried out by collecting the imaging result of the projection component. Wherein, the obturation component comprises a first shell and a first inner cylinder, the first inner cylinder is embedded in the first shell, the first shell can rotate relative to the first inner cylinder, the first shell is provided with a first opening part, the first inner cylinder is provided with a second opening part, when the first inner cylinder rotates to a preset position, the first opening part coincides with the second opening part, so that the obturation component forms a window, the bacteria box can be placed in the first inner cylinder or taken out from the inside of the first inner cylinder through the window, when the first inner cylinder deviates from the preset position, the first opening part and the second opening part are not overlapped, the side wall of the first inner cylinder can shield the first opening part, the side wall of the first shell can shield the second opening part, so that the inside of the first inner cylinder forms an obturation space.
It is thus clear that the bacterium calculates image acquisition device that this application provided is through placing the fungus box in inclosed space, and the light that the light source sent can be concentrated shine to the fungus box, avoids light to run off, utilizes the light source in the at utmost, ensures illumination intensity for light shines can clearly show to the image of bacterial colony formation, avoids the image bacterial colony that becomes to lose, therefore ensures that the later stage carries out the accuracy of the result of counting to the bacterial colony.
Drawings
In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings needed to be used in the detailed description of the present application or the prior art description will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a bacteria calculation image acquisition device provided in an embodiment of the present application;
fig. 2 is a schematic partial structural diagram of a bacteria calculation image acquisition device provided in the embodiment of the present application.
Reference numerals:
1-projection component, 11-second inner cylinder, 111-projection screen, 12-second shell, 2-sealing component, 21-first shell, 211-first opening part, 212-plug part, 22-first inner cylinder, 221-twist ring, 3-lamp box seat, 31-third shell, 4-objective table, 41-holding tank, 42-baffle, 5-concentric pressing clamp, 6-camera, 7-arc line box and 8-holding frame.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.
In the description of the embodiments of the present application, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that the products of the present invention are usually placed in when used, and are only used for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical" and the like do not imply that the components are required to be absolutely horizontal or pendant, but rather may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the embodiments of the present application, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As used herein, the term "and/or" includes any one of the associated listed items and any combination of any two or more of the items.
For ease of description, spatial relationship terms such as "above … …," "upper," "below … …," and "lower" may be used herein to describe one element's relationship to another element as illustrated in the figures. Such spatial relationship terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures.
The terminology used herein is for the purpose of describing various examples only and is not intended to be limiting of the disclosure. The singular forms also are intended to include the plural forms as well, unless the context clearly indicates otherwise. The terms "comprises," "comprising," and "having" specify the presence of stated features, quantities, operations, elements, components, and/or combinations thereof, but do not preclude the presence or addition of one or more other features, quantities, operations, components, elements, and/or combinations thereof.
Variations from the shapes of the illustrations as a result, for example, of manufacturing techniques and/or tolerances, may be expected. Thus, the examples described herein are not limited to the particular shapes shown in the drawings, but include changes in shape that occur during manufacturing.
The features of the examples described herein may be combined in various ways that will be apparent after understanding the disclosure of the present application. Further, while the examples described herein have a variety of configurations, other configurations are possible, as will be apparent after understanding the disclosure of the present application. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present application.
The bacteria calculation image acquisition apparatus according to some embodiments of the present application is described below with reference to fig. 1 and 2. FIG. 1 is a schematic structural diagram of a bacteria calculation image acquisition device provided in an embodiment of the present application; fig. 2 is a schematic partial structural diagram of a bacteria calculation image acquisition device provided in the embodiment of the present application.
Referring to fig. 1 and 2, an embodiment of the present application provides a bacteria calculation image acquisition apparatus including: a projection unit 1 and a sealing unit 2 connected to the projection unit 1;
the sealing member 2 includes a first housing 21 and a first inner cylinder 22, and the first housing 21 is rotatable with respect to the first inner cylinder 22;
the first shell 21 is provided with a first opening 211, the first inner cylinder 22 is provided with a second opening, and when the first inner cylinder 22 rotates to a preset position, the first opening 211 and the second opening coincide to place the fungus box in the first inner cylinder 22;
when the first inner cylinder 22 is deviated from a predetermined position, the inside of the first inner cylinder 22 forms a closed space.
The application provides a bacteria calculation image acquisition device includes: the bacterial box is placed in the sealing component 2, the light source can irradiate the bacterial box to form an image, the formed image can be projected to the projection component 1, and the subsequent bacterial colony counting can be carried out by collecting the imaging result of the projection component 1. The sealing member 2 includes a first housing 21 and a first inner cylinder 22, the first inner cylinder 22 is embedded in the first housing 21, the first housing 21 can rotate relative to the first inner cylinder 22, the first housing 21 is provided with a first opening 211, the first inner cylinder 22 is provided with a second opening, when the first inner cylinder 22 rotates to a predetermined position, the first opening 211 coincides with the second opening, so that the sealing member 2 forms a window, the fungus box can be placed in the first inner cylinder 22 or taken out from the inside of the first inner cylinder 22 through the window, when the first inner cylinder 22 deviates from the predetermined position, the first opening 211 and the second opening are not overlapped, the side wall of the first inner cylinder 22 can shield the first opening 211, the side wall of the first housing 21 can shield the second opening, so that a sealed space is formed inside the first inner cylinder 22.
It is thus clear that the bacterium calculates image acquisition device that this application provided is through placing the fungus box in inclosed space, and the light that the light source sent can shine to the fungus box concentratedly, avoids light to run off, utilizes the light source in the at utmost, ensures illumination intensity for light shines can clearly show to the image of bacterial colony formation, avoids the image bacterial colony that becomes to lose, therefore ensures that the later stage carries out the accuracy of the result of counting to the bacterial colony.
It should be noted that the bacteria calculation image collecting device further includes a supporting frame 8, the supporting frame 8 is used for supporting and placing the bacteria calculation image collecting device, so as to prevent the bacteria calculation image collecting device from rolling due to smooth appearance, optionally, the supporting frame 8 is disposed at the bottom of the first housing 21, which is not limited thereto, and the supporting frame 8 and the first housing 21 may be an integrated structure or a split structure.
In one embodiment of the present application, preferably, as shown in fig. 1, the first housing 21 has a cylindrical structure with one end open, and the one end of the first housing 21 is open to form the insertion part 212.
Further, the first inner cylinder 22 has a cylindrical structure, and one end of the first inner cylinder 22 is provided with a screw ring 221, and the screw ring 221 is projected relative to the surface of the first inner cylinder 22, and the screw ring 221 can cover the plug portion 212 in a state where the first inner cylinder 22 is fitted in the first housing 21.
In this embodiment, the screw ring 221 is disposed at one end of the first inner cylinder 22 close to the projection component 1, the screw ring 221 protrudes relative to the surface of the first inner cylinder 22, so that an accommodating space is formed between the screw ring 221 and the first inner cylinder 22, after the first inner cylinder 22 is mounted on the first housing 21, the insertion part 212 of the first housing 21 is accommodated in the accommodating space, the screw ring 221 can cover the connection position of the first inner cylinder 22 and the first housing 21, light transmission is avoided, the sealing effect is not affected, and the first inner cylinder 22 can be rotated by operating the screw ring 221.
It should be noted that the inside of the first inner cylinder 22 is further provided with an arc-shaped line box 7, and the arc-shaped line box 7 is arranged avoiding the light path and is used for accommodating a power line and a signal transmission line of the camera 6.
In one embodiment of the present application, preferably, as shown in fig. 1, the light box base 3 is further included, the light box base 3 includes a third housing 31 and a base, the third housing 31 is detachably connected to the second end of the first housing 21, and the base is provided with a light source.
Further, an accommodation space is formed between the third housing 31 and the base for accommodating a power supply.
In this embodiment, the light source may be a tungsten lamp, but is not limited thereto, and when the base is assembled to the third housing 31, the light source approaches the filter and is in close proximity to the filter, thereby utilizing the light source to the maximum extent. The power supply is accommodated between the third shell 31 and the base to form an accommodating space, the power supply supplies power to the light source and the camera 6, the internal wiring of the bacteria calculation image acquisition device from the outside is avoided, the number of wiring holes is reduced, and the light-blocking effect is ensured.
In one embodiment of the present application, preferably, as shown in fig. 1 and 2, the third housing 31 has a cylindrical structure with one end open, the stage 4 is provided at a bottom of the third housing 31 opposite to the opening, the stage 4 is located inside the first inner cylinder 22 in a state where the first housing 21 is assembled to the third housing 31, and the stage 4 is formed with a housing groove 41 for housing the fungi box.
Further, the objective table 4 is provided with a hollow pressing clamp 5, and the hollow pressing clamp 5 is used for fixing the fungus box in the accommodating groove 41.
In this embodiment, the clamp 5 is pressed to the same heart as the support column and is drawn the clamp, and the support column sets up in objective table 4, and the pulling is drawn the clamp, alright will place the fungus box in holding tank 41, loosens afterwards and draws the clamp, alright fix the fungus box to need to explain, still be provided with separation blade 42 in the holding tank 41 for the fixed fungus box of clamp 5 is pressed to the same heart to the cooperation, avoids the even fungus box that leads to of clamping force that the clamp 5 was pressed to the fungus box of being flush to the same heart to shift.
In an embodiment of the present application, preferably, the bottom of the third housing 31 is opened with a light hole and a mounting portion, the mounting portion is used for fixing the filter, and the light hole is used for allowing the light filtered by the filter to enter the inside of the first inner cylinder 22 and irradiate the fungus box.
In this embodiment, light shines to the fungus box through the filter, and different bacterial colonies can show different colours through different filters, and the kind of bacterial colony is distinguished to the accessible colour, and simultaneously, the through wires hole has still been seted up to the bottom of first inner tube 22 for pass camera 6's power cord and signal transmission line, make the circuit walk the line in this bacterium calculation image collection system's inside, avoid the light leak, and wherein, the installation department can set up in the lateral surface of the bottom of third shell 31.
In one embodiment of the application, the object table 4 is preferably provided with a camera 6, as shown in fig. 1, the lens of the camera 6 being directed towards the projection member 1.
Further, the projection member 1 includes a second inner cylinder 11 and a second housing 12, the second housing 12 is detachably connected to the first end of the first housing 21, the second inner cylinder 11 is screw-connected to the second housing 12, and the second inner cylinder 11 is provided with a projection screen 111.
In this embodiment, the objective table 4 is provided with a support frame 8 for fixing the camera 6, the camera 6 is hinged to the fixing frame for adjusting the lens orientation of the camera 6, and the number of the camera 6 can be one or more, and can be set according to practical situations, the lens of the camera 6 is set towards the projection screen 111, the projection screen 111 is set on the opening of one end of the second inner cylinder 11 for receiving images, wherein, preferably, the projection screen 111 is set on the opening of one end of the second inner cylinder 11 away from the sealing member 2, the images formed by the light irradiation fungi box are projected onto the projection screen 111, the camera 6 can record the imaging result on the projection screen 111 and transmit the result to a processing terminal such as a computer, and the processing terminal can calculate the order corresponding to the number of detected colonies after analyzing the information collected by the camera 6, colony counts were achieved.
Finally, it should be noted that: the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.
Claims (10)
1. A bacteria calculation image acquisition device comprising projection means for imaging, characterized by further comprising: a sealing member connected to the projection member;
the sealing component comprises a first outer shell and a first inner cylinder, and the first outer shell can rotate relative to the first inner cylinder;
the first shell is provided with a first opening part, the first inner cylinder is provided with a second opening part, and when the first inner cylinder rotates to a preset position, the first opening part and the second opening part are overlapped and used for placing the fungus box in the first inner cylinder;
when the first inner cylinder deviates from the preset position, a closed space is formed inside the first inner cylinder.
2. The bacteria calculation image acquisition apparatus according to claim 1, wherein the first housing has a cylindrical structure with one end open, and wherein one end opening of the first housing is formed with a socket.
3. The bacteria calculation image acquisition apparatus according to claim 2, wherein the first inner cylinder has a cylindrical structure, a screw ring is provided at one end of the first inner cylinder, the screw ring is raised with respect to a surface of the first inner cylinder, and the screw ring can cover the insertion portion in a state where the first inner cylinder is fitted in the first housing.
4. The bacteria calculation image collection device according to any one of claims 1 to 3, further comprising a light box base including a third housing and a base, the third housing being detachably connected to the second end of the first housing, the base being provided with a light source.
5. The bacteria-calculating image capturing apparatus according to claim 4, wherein the third housing has a cylindrical structure with one end open, a stage is provided on a bottom portion of the third housing opposite to the opening, the stage is located inside the first inner tube in a state where the first housing is fitted to the third housing, and a receiving groove is formed in the stage to receive the bacteria cassette.
6. The bacteria calculation image acquisition device according to claim 4, wherein a light hole and a mounting portion are formed in the bottom of the third housing, the mounting portion is used for fixing a filter, and the light hole is used for enabling light filtered by the filter to enter the first inner barrel and irradiate the bacteria box.
7. The bacteria calculation image collection device of claim 5, wherein the stage is provided with a concentric pressing clamp for fixing the bacteria box in the accommodating groove.
8. The bacteria computed image acquisition apparatus according to claim 5, wherein the stage is provided with a camera having a lens directed toward the projection member.
9. The bacteria calculation image collection device according to any one of claims 1 to 3, wherein the projection member includes a second inner tube and a second housing, the second housing is detachably connected to the first end of the first housing, the second inner tube is screwed to the second housing, and the second inner tube is provided with a projection screen.
10. The bacteria calculation image acquisition device according to claim 4, wherein a storage part is formed between the third housing and the base for storing a power supply.
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| CN202010092062.5A CN111269815B (en) | 2020-02-12 | 2020-02-12 | Image acquisition device for bacteria calculation |
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| CN202010092062.5A CN111269815B (en) | 2020-02-12 | 2020-02-12 | Image acquisition device for bacteria calculation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111996110A (en) * | 2020-08-27 | 2020-11-27 | 迪泰伊诺(天津)科技有限公司 | Water quality bacteria detection and analysis device |
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| CN209854121U (en) * | 2019-02-01 | 2019-12-27 | 北京陆桥技术股份有限公司 | Colony image acquisition system |
| CN110656040A (en) * | 2019-11-08 | 2020-01-07 | 河北冠卓检测科技股份有限公司 | Bacterial colony counting device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111996110A (en) * | 2020-08-27 | 2020-11-27 | 迪泰伊诺(天津)科技有限公司 | Water quality bacteria detection and analysis device |
| CN111996110B (en) * | 2020-08-27 | 2023-08-01 | 迪泰伊诺(天津)科技有限公司 | Water quality bacteria detection and analysis device |
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