CN218823937U

CN218823937U - Multi-light-source gel imaging system

Info

Publication number: CN218823937U
Application number: CN202320109620.3U
Authority: CN
Inventors: 王卫伟; 罗文波; 梁中杰
Original assignee: Guangzhou Bolutang Biotechnology Co ltd
Current assignee: Guangzhou Bolutang Biotechnology Co ltd
Priority date: 2023-01-16
Filing date: 2023-01-16
Publication date: 2023-04-07
Anticipated expiration: 2033-01-16

Abstract

The utility model discloses a many light sources gel imaging system, include: the object stage is internally provided with a containing cavity, and the surface of the object stage is provided with a light-transmitting window which is positioned above the containing cavity and used for placing a sample; the light source module comprises LED lamp beads arranged on the circuit board and a light-shading shell covering the LED lamp beads, and the upper end of the light-shading shell is provided with a light filter plate corresponding to the LED lamp beads; and the camera is arranged above the light-transmitting window and used for carrying out exposure imaging when the light source assembly irradiates the sample. The utility model provides a many light sources gel imaging system has compromise the needs of multiple different light sources, need not any manually operation, and automatic shooting formation of image uses more convenient and safety.

Description

Multi-light-source gel imaging system

Technical Field

The utility model belongs to the technical field of optical imaging, concretely relates to many light sources gel imaging system.

Background

Fluorescence detection is an important method for molecular biology research. Fluorescent dyes produce light emission of a specific wavelength when excited by irradiation with a light source such as visible light or ultraviolet light. Therefore, in the molecular biology research, the fluorescent dye is combined with a detection sample, then the fluorescent dye is irradiated by using a corresponding excitation light source for excitation, and qualitative and quantitative analysis of the sample is realized by detecting the light intensity of the emission wavelength emitted by the fluorescent dye.

According to the fluorescent probes and detection methods required by different samples in fluorescence detection, common excitation light sources can be roughly divided into two types, namely an ultraviolet light source and a blue light source for nucleic acid detection, and a white light source for protein-silver staining, protein-Coomassie brilliant blue and bacterial colony counting, wherein the former is generally composed of an LED or a fluorescent lamp tube and a filter plate, and the latter is composed of an LED and a light homogenizing plate or converted by other light sources.

Related products in the market are roughly divided into three types, one type is that only one ultraviolet or blue light source is arranged, and then the ultraviolet or blue light source is converted into a white light source through a white light conversion plate, so that double light sources of ultraviolet or blue light and white light are formed. The second type is to do ultraviolet light source module, blue light source module and white light source module respectively, and during the use, which kind of light source module needs, and equipment is packed into to it manually, and the light source module that does not use will be torn open out, and troublesome poeration, customer and light source module are too much contacted, increase biological reagent and infect the risk. The third type is to uniformly distribute the three LED lamps on the same circuit board, and then manually replace the ultraviolet filter plate, the blue light filter plate and the white light homogenizing plate.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome current technical defect, provide a many light sources gel imaging system to solve the problem of the unable compatible use of multiple light source.

In order to solve the above technical problem, the utility model provides a many light sources gel imaging system, include:

the object stage is internally provided with a containing cavity, and the surface of the object stage is provided with a light-transmitting window which is positioned above the containing cavity and used for placing a sample;

the light source assembly is arranged in the containing cavity and positioned below the light transmission window so as to be used for irradiating a sample on the light transmission window, the light source assembly comprises a circuit board with the size larger than that of the light transmission window and a plurality of rows of light source modules which are arranged on the circuit board and used for respectively emitting light with different colors, each light source module comprises an LED lamp bead arranged on the circuit board and a light-proof shell covering the LED lamp bead, and the upper end of the light-proof shell is provided with a light filter plate corresponding to the LED lamp bead;

and the camera is arranged above the light-transmitting window and used for carrying out exposure imaging when the light source assembly irradiates the sample.

Furthermore, the number of the light source modules in each row is one or more, so that the light source modules are uniformly distributed on the circuit board in a strip shape or a point shape.

Furthermore, when the light source modules are distributed in a dotted manner, one or more LED lamp beads are arranged in the light-shielding shell.

Further, when the light source modules are distributed in a strip shape, the light-shielding shell covers at least one row of LED lamp beads.

Furthermore, the circuit board is provided with a plurality of rows of light source modules which are respectively used for emitting blue light, ultraviolet light and white light and are arranged in a staggered manner.

Furthermore, the objective table comprises a base with a containing cavity and a cover plate covering the base, and the middle of the cover plate is provided with a light-transmitting window for transmitting light.

Further, the light-transmitting window is made of transparent glass or acrylic plates.

Further, a lens is arranged at the lower end of the camera.

Furthermore, a filter or a filter wheel with a plurality of filters with different wavelengths is arranged below the lens.

The utility model discloses following beneficial effect has:

the utility model discloses in, set up the light source subassembly that has the light source module that can send different colour light source module in the objective table, the area source integration of a plurality of different wavelengths is in the same place, compromise the sample of multiple excitation light source wavelength, it is required to realize various detections, and take the filter plate certainly among the light source module, need not pass through the filter plate of manual change different colour light when using different colour light, need not any other manual operation, overall structure is simple, high durability and convenient operation, only need to treat that the sample is placed on the objective table, again according to treating the type and the used fluorescence probe of treating the sample, only need open the light source module that corresponds the excitation light colour shine can, the camera of cooperation top, automatic shooting exposure imaging, it is more convenient and safe to use.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, do not constitute a limitation of the invention, and in which:

FIG. 1 is a schematic diagram of a multi-light source gel imaging system in an embodiment;

FIG. 2 is a schematic view illustrating a point-like distribution of light source modules according to an embodiment;

FIG. 3 is a schematic structural diagram of a point light source module according to an embodiment;

FIG. 4 is a schematic view illustrating light source modules distributed in a stripe shape according to another embodiment;

fig. 5 is a schematic structural diagram of a strip light source module in another embodiment.

Detailed Description

In order to fully understand the technical contents of the present invention, the present invention will be further described and explained with reference to the accompanying drawings and specific embodiments; it should be noted that, if "first" and "second" are described in the text, they are used to distinguish different components, and do not represent a sequential order, and do not limit "first" and "second" to be different types.

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

Examples

As shown in fig. 1, the multi-light-source gel imaging system shown in this embodiment includes an object stage, a light source assembly 9, and a camera 7, wherein a cavity 4 is provided inside the object stage, and a light-transmitting window 3 is provided on a surface of the object stage, the light-transmitting window being located above the cavity and used for placing a sample to be detected, so that light can conveniently irradiate on the sample to be detected through the light-transmitting window 3; the light source assembly is arranged in the containing cavity 4 and is positioned below the light transmission window 3, and is used for irradiating the sample to be detected on the light transmission window 3 from bottom to top so as to excite the fluorescent probe on the sample to be detected; the light source component comprises a circuit board and a plurality of rows of light source modules which are arranged on the circuit board and used for respectively emitting blue light, ultraviolet light and white light, wherein the size of the circuit board is larger than that of a light transmission window, so that the light emitted by the light source modules on the circuit board can cover the whole light transmission window, the light source modules comprise LED lamp beads 15 arranged on the circuit board 10 and a light-shielding shell 14 covered on the LED lamp beads 15, the upper end of the light-shielding shell 14 is provided with a light filter plate 16 corresponding to the LED lamp beads 15, therefore, the adopted light filter plates have different colors according to the different colors of the covered LED lamp beads, and the light source modules can be divided into a blue light LED light source module 11, an ultraviolet LED light source module 12 and a white light LED light source module 13, so that the light source module of which light is adopted can be selected according to exciting light required by a fluorescent probe in a sample to be detected; the camera 7 is arranged right above the light-transmitting window 3 and used for exposure imaging when the light source assembly irradiates a sample to be detected.

It can be understood that the light source module can also select the LED lamp beads with other wavelength colors and the corresponding matched filter plate according to actual needs, so as to realize the detection requirements of wider samples.

In an embodiment, as shown in fig. 2 and 3, the blue LED light source modules 11, the ultraviolet LED light source modules 12, and the white LED light source modules 13 are arranged in an interlaced manner, and each row of the blue LED light source modules 11, the ultraviolet LED light source modules 12, and the white LED light source modules 13 includes a plurality of light source modules, so that the light source modules are uniformly distributed on the circuit board 10 in a dotted manner.

Preferably, when the light source modules are distributed in a dotted manner, one or more LED lamp beads in the light-shielding shell in each light source module are provided, that is, a light-shielding shell can be covered on each LED lamp bead of the same row of the same light source to form the dotted light source module with a single lamp bead structure, or a light-shielding shell can be covered on every two or more LED lamp beads of the same row of the same light source to form the dotted light source module with a multi-lamp bead structure.

In another embodiment, as shown in fig. 4 and 5, the blue LED light source modules 11, the ultraviolet LED light source modules 12, and the white LED light source modules 13 are arranged in an interlaced manner, and there is only one blue LED light source module 11, one ultraviolet LED light source module 12, and one white LED light source module 13 in each row, so that the light source modules are distributed on the circuit board 10 in a strip shape and arranged along the length or width of the circuit board 10.

Preferably, when the light source modules are distributed in a strip shape, the light-shielding shell covers at least one row of the LED lamp beads to form a strip-shaped light source module with a multi-lamp-bead structure, that is, when two adjacent rows of the LED lamp beads are different in color, the light-shielding shell in each strip-shaped light source module only covers a single row of the same-color LED lamp beads, but when two or more adjacent rows of the LED lamp beads are the same in color, the light-shielding shell in each strip-shaped light source module can only cover a single row of the same-color LED lamp beads, or can cover two or more rows of the same-color LED lamp beads.

In this embodiment, as shown in fig. 1, the object stage includes a base 1 having a cavity 4 and a cover plate 2 covering the base 1, and a light-transmitting window 3 for transmitting light is disposed in the middle of the cover plate 2; preferably, the light-transmitting window 3 is made of transparent glass or acrylic plate, so as to facilitate light transmission.

In this embodiment, as shown in fig. 1, a lens 6 is disposed at the lower end of the camera 7, and a filter 5 or a filter wheel with a plurality of filters with different wavelengths is disposed below the lens 6, so that the filters with different wavelengths can be replaced by rotating the filter wheel according to different needs.

When the multi-light-source gel imaging system works, a sample 3 to be detected (usually DNA glue, protein glue and the like) is placed on a light-transmitting window of an objective table, light source modules with corresponding colors in a light source component and optical filter wavelengths below a camera are selected to be started according to the type of the sample to be detected and a used fluorescent probe, and a complete exposure image of the sample to be detected is obtained by clicking and shooting.

The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and embodiments of the present invention are explained herein by using specific examples, and the descriptions of the above embodiments are only applicable to help understand the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the description should not be construed as a limitation to the present invention.

Claims

1. A multiple light source gel imaging system, comprising:

2. The multiple light source gel imaging system of claim 1, wherein one or more of the light source modules in each row are arranged such that the light source modules are uniformly distributed on the circuit board in stripes or dots.

3. The multi-light-source gel imaging system according to claim 2, wherein when the light source modules are distributed in a point shape, one or more LED lamp beads are arranged in the light-shielding shell.

4. The multi-light-source gel imaging system according to claim 2, wherein the light-shielding housing covers at least one row of LED beads when the light source modules are distributed in a stripe shape.

5. The multiple light source gel imaging system of claim 1 wherein the circuit board has rows of light source modules arranged in a staggered configuration for emitting blue, ultraviolet, and white light, respectively.

6. The multiple-light-source gel imaging system according to any one of claims 1 to 5, wherein the stage comprises a base having a cavity and a cover plate covering the base, and the cover plate is provided with the light-transmitting window for transmitting light in the middle.

7. The multiple light source gel imaging system of claim 6, wherein the light transmissive window is made of transparent glass or acrylic.

8. The multiple light source gel imaging system of claim 6 wherein the lower end of the camera is provided with a lens.

9. The multiple light source gel imaging system of claim 8, wherein a filter or a filter wheel with filters of different wavelengths is disposed below the lens.