CN115855910A - Light source scanning type gel imaging system - Google Patents
Light source scanning type gel imaging system Download PDFInfo
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- CN115855910A CN115855910A CN202310063331.9A CN202310063331A CN115855910A CN 115855910 A CN115855910 A CN 115855910A CN 202310063331 A CN202310063331 A CN 202310063331A CN 115855910 A CN115855910 A CN 115855910A
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Abstract
The invention discloses a light source scanning type gel imaging system, which comprises: the object stage is internally provided with a containing cavity, and the surface of the object stage is provided with a light-transmitting window which is positioned above the containing cavity and used for placing a sample; the light source assembly is arranged in the containing cavity and arranged along the short side of the light transmission window so as to be used for irradiating a sample on the light transmission window, and the light source assembly comprises at least three rows of light source modules which are arranged in front and back and used for respectively emitting blue light, ultraviolet light and white light; the driving device is used for driving the light source component to move along the long edge of the light-transmitting window; and the camera is arranged above the light-transmitting window and used for carrying out continuous exposure imaging when the light source assembly moves, scans and irradiates. The light source scanning type gel imaging system meets the requirements of various light sources, does not need any manual operation, automatically scans and images, and is more convenient and safer to use.
Description
Technical Field
The invention belongs to the technical field of optical imaging, and particularly relates to a light source scanning type gel imaging system.
Background
Fluorescence detection is an important method for molecular biology research. Fluorescent dyes produce light emission of a specific wavelength when excited by irradiation with a light source such as visible light or ultraviolet light. Therefore, in the molecular biology research, the fluorescent dye is combined with a detection sample, then the fluorescent dye is irradiated by using a corresponding excitation light source for excitation, and qualitative and quantitative analysis of the sample is realized by detecting the light intensity of the emission wavelength emitted by the fluorescent dye.
According to the fluorescent probes and detection methods required by different samples in fluorescence detection, common excitation light sources can be roughly divided into two types, namely an ultraviolet light source and a blue light source for nucleic acid detection, and a white light source for protein-silver staining, protein-Coomassie brilliant blue and bacterial colony counting, wherein the former is generally composed of an LED or a fluorescent lamp tube and a filter plate, and the latter is composed of an LED and a light homogenizing plate or converted by other light sources.
Related products on the market are roughly divided into three types, one type is that only one ultraviolet or blue light source is arranged, and then the ultraviolet or blue light source is converted into a white light source through a white light conversion plate, so that double light sources of ultraviolet or blue light and white light are formed. The second type is that the ultraviolet light source module, the blue light source module and the white light source module are respectively made, when the device is used, which light source module is needed, the device is manually installed in the device, the light source modules which are not used need to be detached, the operation is troublesome, the customers and the light source modules are in too much contact, and the infection risk of the biological reagent is increased. And the third type is that three LED lamps are uniformly distributed on the same circuit board, and then the ultraviolet filter plate, the blue light filter plate and the white light homogenizing plate are manually replaced.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a light source scanning type gel imaging system with three light sources.
In order to solve the above technical problem, the present invention provides a light source scanning type gel imaging system, comprising:
the object stage is internally provided with a containing cavity, and the surface of the object stage is provided with a light-transmitting window which is positioned above the containing cavity and used for placing a sample;
the light source assembly is arranged in the containing cavity and arranged along the short edge of the light-transmitting window so as to be used for irradiating a sample on the light-transmitting window, and the light source assembly comprises at least three rows of light source modules which are arranged in front and back and are used for respectively emitting blue light, ultraviolet light and white light;
the driving device is used for driving the light source component to move along the long edge of the light-transmitting window;
and the camera is arranged above the light-transmitting window and used for carrying out continuous exposure imaging when the light source assembly moves, scans and irradiates.
Furthermore, each row the light source module includes along lamp plate and a plurality of interval LED lamp pearls on locating the lamp plate that the minor face of printing opacity window set up.
Further, the light source module further comprises a support for accommodating the lamp panel, and a filter plate is arranged on one side of the support corresponding to the LED lamp beads.
Further, the length of the light source module is larger than or equal to the length of the short side of the light-transmitting window.
Furthermore, the objective table comprises a base with a containing cavity and a cover plate covering the base, and the middle of the cover plate is provided with a light-transmitting window for transmitting light.
Further, the light-transmitting window is made of transparent glass or acrylic plates.
Furthermore, drive arrangement is including rotating locate holding the intracavity and following the lead screw that the long limit of printing opacity window set up, locate the screw nut on the lead screw and drive lead screw pivoted motor, the light source subassembly is fixed in on the screw nut.
Further, a lens is arranged at the lower end of the camera.
Furthermore, a filter or a filter wheel with a plurality of filters with different wavelengths is arranged below the lens.
Further, the camera is an area-array camera or a line-array camera.
The invention has the following beneficial effects:
according to the invention, the light source component with the light source modules with three different colors is arranged in the objective table to realize various detection requirements, and the light source component consisting of the three light source modules moves under the driving of the driving device, so that a scanning type irradiation light source is formed, and thus, aiming at different sample detections, only the corresponding light source module needs to be started to move, scan and irradiate.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention:
FIG. 1 is a schematic diagram of an exemplary light source scanning gel imaging system;
FIG. 2 is a schematic diagram illustrating a light source assembly of a scanning gel imaging system according to an embodiment after moving;
FIG. 3 is a schematic diagram of a light source module according to an embodiment;
fig. 4 is a schematic view of the lamp panel in the embodiment.
Detailed Description
For a fuller understanding of the technical aspects of the present invention, reference should be made to the following detailed description taken together with the accompanying drawings; it should be noted that, if "first" or "second" is described in the text, it is used to distinguish different components, and the like, and does not represent the order of precedence, and does not limit "first" and "second" to be different types.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
As shown in fig. 1 to 4, the light source scanning type gel imaging system shown in this embodiment includes an object stage, a light source assembly, a driving device, and a camera 11, wherein a cavity 8 is disposed inside the object stage, and a light-transmitting window 1 is disposed on a surface of the object stage, and is located above the cavity 8 and used for placing a sample 7 to be detected, so that light can be conveniently irradiated onto the sample to be detected through the light-transmitting window; the light source assembly is arranged in the containing cavity 8 and arranged along the short edge of the light-transmitting window 1, so that the light source assembly can cover and irradiate the short edge of the whole light-transmitting window 1 so as to irradiate a sample to be detected on the light-transmitting window 1 from bottom to top and excite a fluorescent probe on the sample to be detected, the light source assembly comprises three rows of light source modules 6 which are arranged in front and at the back and are used for respectively emitting blue light, ultraviolet light and white light, namely the light source assembly can respectively emit the blue light, the ultraviolet light and the white light, and the light source modules which adopt the light can be selected according to the exciting light required by the fluorescent probe in the sample to be detected; the driving device is used for driving the light source component to move along the long edge of the light-transmitting window 1, so that the light source component performs scanning irradiation along the long edge of the light-transmitting window 1 to form a scanning type light source and can scan the whole sample to be detected on the light-transmitting window 1; the camera 1 is arranged right above the light-transmitting window 1 and used for continuous exposure imaging when the light source assembly moves, scans and irradiates.
It is understood that the light source module 6 may also extend along the long side of the light-transmissive window, and the driving device drives the light source assembly to move along the short side of the light-transmissive window, in which the moving distance is shorter, but the cost of the light source module is increased.
Preferably, as shown in fig. 4, each row of light source modules 6 includes a lamp panel 14 arranged along a short side of the light-transmitting window 1 and a plurality of LED lamp beads 15 arranged along the length of the lamp panel at intervals; in a specific embodiment, the colors emitted by the LED lamp beads on the three rows of light source modules are different according to the three light sources, namely, blue light, ultraviolet light and white light, so as to form a light source assembly with three different light sources.
Preferably, as shown in fig. 3, the light source module 6 further includes a support 13 for accommodating the lamp panel 14, a filter plate 12 is disposed at one side of the support 13 corresponding to the LED lamp beads 15, the filter plate 12 is used to filter out unwanted light, and only excitation light with a desired color is transmitted outwards, so that the LED lamp beads adopted in the three rows of light source modules 6 may be the same, and only the filter plates adopted in the three rows of light source modules 6 need to be different, that is, the wavelengths of light sources transmitted by the three filter plates are different, so as to respectively emit blue light, ultraviolet light, and white light, thereby forming a light source assembly with three different light sources.
In this embodiment, the driving device includes a screw 8 rotatably disposed in the cavity 8 and disposed along the long side of the light-transmitting window 1, a screw nut (not shown in the figure) disposed on the screw 8, and a motor 5 for driving the screw 8 to rotate, and the light source assembly is fixed on the screw nut; of course, in order to achieve smooth sliding and rotation avoidance of the light source assembly, guide rails (not shown in the figure) are respectively arranged on two sides of the screw rod 8, and two ends of the light source assembly are fixed on the guide rails through sliding blocks.
In this embodiment, the long limit of whole light-transmitting window is covered in the removal stroke of light source subassembly in holding the intracavity, and the length of light source module 6 is greater than or equal to the minor face length of light-transmitting window 1, can ensure like this that whole light-transmitting window part all can be shone, avoids shining incompletely and influence formation of image and detect the reliability, is applicable to various different specification sizes and waits to detect the sample, and the biggest applicable specification is the size of light-transmitting window.
In the embodiment, the objective table comprises a base 3 with a containing cavity 8 and a cover plate 2 covering the base 3, wherein a light-transmitting window 1 for transmitting light is arranged in the middle of the cover plate 2; preferably, the light-transmitting window 1 is made of transparent glass or acrylic plate to facilitate light transmission.
In this embodiment, a lens 10 is disposed at the lower end of the camera 11, and a filter 9 or a filter wheel with a plurality of filters with different wavelengths is disposed below the lens 10, so that the filters with different wavelengths can be replaced by rotating the filter wheel according to different requirements.
Preferably, the camera can be an area-array camera or a line-array camera, and can achieve the purpose of scanning and imaging; when the camera is an area-array camera, the area-array camera is continuously exposed to obtain a picture in the process of one-time moving scanning of the light source; when the camera is a linear array camera, in the process of one-time moving scanning of the light source, a group of pictures are obtained by exposure section by section, and then image splicing is carried out to obtain a similar area array picture shot by the area array camera.
When the light source scanning type gel imaging system works, a sample 3 to be detected (usually DNA glue, protein glue and the like) is placed on a light-transmitting window of an objective table, the wavelength of a light filter below a corresponding light source module and a camera in a light source assembly is selected to be started according to the type of the sample to be detected and a fluorescent probe used, clicking shooting is carried out, the light source assembly is driven by a driving device to move from a starting point on one side to an end point on the other side at a constant speed (as shown in figures 1 and 2), meanwhile, the camera performs continuous exposure when the light source assembly starts to move until the light source assembly moves to the end point on the other side, namely, the light source scans to the end point from the starting point, the camera also scans and excites the other side from one side of the sample to be detected, and finally obtains a complete exposure image of the sample to be detected.
The technical solutions provided by the embodiments of the present invention are described in detail above, and specific examples are applied herein to explain the principles and embodiments of the present invention, and the descriptions of the embodiments above are only used to help understanding the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the present description should not be construed as a limitation to the present invention.
Claims (10)
1. A light source scanning gel imaging system, comprising:
the object stage is internally provided with a containing cavity, and the surface of the object stage is provided with a light-transmitting window which is positioned above the containing cavity and used for placing a sample;
the light source assembly is arranged in the containing cavity and arranged along the short side of the light transmission window so as to be used for irradiating a sample on the light transmission window, and the light source assembly comprises at least three rows of light source modules which are arranged in front and back and used for respectively emitting blue light, ultraviolet light and white light;
the driving device is used for driving the light source component to move along the long edge of the light-transmitting window;
and the camera is arranged above the light-transmitting window and used for continuously exposing and imaging when the light source assembly moves, scans and irradiates.
2. The light source scanning gel imaging system of claim 1, wherein each row of the light source modules comprises a lamp panel arranged along a short side of the light-transmissive window and a plurality of LED lamp beads arranged on the lamp panel at intervals.
3. The light source scanning type gel imaging system according to claim 2, wherein the light source module further comprises a bracket for accommodating a lamp panel, and a filter plate is arranged on one side of the bracket corresponding to the LED lamp bead.
4. The light source scanning gel imaging system of claim 3, wherein the length of the light source module is greater than or equal to the length of the short side of the light transmissive window.
5. The light source-scanning gel imaging system of claim 4, wherein the stage comprises a base having a cavity and a cover plate covering the base, the cover plate having the light-transmitting window for transmitting light in the middle.
6. The light source scanning gel imaging system of claim 5, wherein the light transmissive window is made of transparent glass or acrylic.
7. The light source scanning gel imaging system of claim 5, wherein the driving device comprises a lead screw rotatably disposed in the cavity and along the long side of the transparent window, a lead screw nut disposed on the lead screw, and a motor for driving the lead screw to rotate, and the light source assembly is fixed on the lead screw nut.
8. The light source scanning gel imaging system of any of claims 1-7, wherein a lens is provided at a lower end of the camera.
9. The light source scanning gel imaging system of claim 8, wherein a filter or a filter wheel with several filters of different wavelengths is disposed under the lens.
10. The light source scanning gel imaging system of claim 9, wherein the camera is an area camera or a line camera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310063331.9A CN115855910A (en) | 2023-01-16 | 2023-01-16 | Light source scanning type gel imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310063331.9A CN115855910A (en) | 2023-01-16 | 2023-01-16 | Light source scanning type gel imaging system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115855910A true CN115855910A (en) | 2023-03-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310063331.9A Pending CN115855910A (en) | 2023-01-16 | 2023-01-16 | Light source scanning type gel imaging system |
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|---|---|
| CN (1) | CN115855910A (en) |
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- 2023-01-16 CN CN202310063331.9A patent/CN115855910A/en active Pending
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