CN2559982Y - CCD biochip detector with uniform lighting system - Google Patents
CCD biochip detector with uniform lighting system Download PDFInfo
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- CN2559982Y CN2559982Y CN 02252864 CN02252864U CN2559982Y CN 2559982 Y CN2559982 Y CN 2559982Y CN 02252864 CN02252864 CN 02252864 CN 02252864 U CN02252864 U CN 02252864U CN 2559982 Y CN2559982 Y CN 2559982Y
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- mirror
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- biochip
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- 238000000018 DNA microarray Methods 0.000 title claims abstract description 27
- 230000003287 optical effect Effects 0.000 claims abstract description 22
- 238000005286 illumination Methods 0.000 claims abstract description 17
- 230000005284 excitation Effects 0.000 claims abstract description 13
- 238000004458 analytical method Methods 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 7
- 238000009413 insulation Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 238000002310 reflectometry Methods 0.000 claims description 2
- 239000007850 fluorescent dye Substances 0.000 abstract description 4
- 229910052724 xenon Inorganic materials 0.000 abstract description 3
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 abstract description 3
- 238000002073 fluorescence micrograph Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 3
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Images
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A CCD biochip detector with uniform lighting system is composed of xenon lamp, uniform lighting optical element, narrow-band exciting filter, narrow-band emitting filter, lens and CCD camera. The target molecules marked with the fluorescent dye generate fluorescence under the excitation of monochromatic light, and are captured and imaged on the CCD by the lens through the narrow-band emission filter. The utility model discloses different with general biochip detecting instrument, be equipped with the even illumination optical element for light intensity uniformity on the biochip has reached 3%, has improved the accuracy of arousing fluorescence greatly, and the accuracy of the fluorescence image who acquires from this also can improve greatly.
Description
Technical field
The utility model relates to a kind of bio-chip test device, particularly has the CCD biochip supplies analysis of uniform illumination system.
Background technology
The enforcement of the Human Genome Project has driven the emergence and the development of emerging biological information industry, the appearance of biochip industry makes on a large scale, the function expression in all cases of analyzing gene becomes possibility expeditiously, can solve the difficulty that is run into the traditional biological section of learning to do effectively.Biochip technology is called the support technology of 21st century life science, is the core technology of portable biochemical analytical instrument, and it will be to have far-reaching scientific and technological revolution again after large scale integrated circuit.
Biochip is meant the biomolecule microarry probe by robot is printed automatically or light guiding chemical synthesising technology is made on silicon chip, glass, gel or nylon membrane, probe on the biochip produces fluoroscopic image after hybridizing with the process fluorescence labeling or through the target sample of enzyme labeling.Fluorescence labeling is that using at most in the chip information collection also is the most successful report sign at present, the fluorescence position of each reflecting point on the chip behind the hybridization reaction, fluorescence is strong and weak converts fluorescence signal to data through chip detecting system, can obtain relevant biological information.
At present, existing C CD biochip supplies analysis all by light source, excite spike interference filter, emission spike interference filter, camera lens and CCD camera to form, its excitation source illuminator has only light source is converged to effect on the chip, and not with the luminous energy homogenising, like this homogeneity of luminous energy do not reach ± 10%.Because the fluorescence that ejects on the biochip is fainter, need the exposure of long period, the inhomogeneous meeting of luminous energy causes the fluorescence that ejects can not reflect biological information really, must compensate by the unevenness that software is measured luminous energy in real time, because this exciting is not linear, software compensation can cause certain error.
Summary of the invention
The utility model technology is dealt with problems and is: a kind of CCD biochip supplies analysis with uniform illumination system is provided, make light intensity uniformity on the biochip reached ± 3%, improved the accuracy of fluorescence excitation widely, the accuracy of the fluoroscopic image that obtains thus also is greatly improved.
Technical solution of the present utility model is: the CCD biochip supplies analysis with uniform illumination system, comprise light source, narrowband excitation optical filter, narrow emission optical filter, camera lens and CCD camera, be characterized in: between light source and narrowband excitation optical filter, be added with even illumination optical device.The light that is come out by light emitted becomes the uniform directional light of a branch of light intensity via even illumination optical device, through exciting spike interference filter (bandwidth is generally tens nanometers), remove by filter the light of other wavelength, to reduce detection background, oblique being mapped on the biochip.The target molecule that is marked with fluorescent dye is at the monochromatic fluorescence that produces down that excites, and caught by pick-up lens through the emission spike interference filter to be imaged on the CCD.Picture signal can directly be sent to image pick-up card in the PCI slot that is inserted in computing machine by the CCD camera, is digital signal with signal transition, carries out Treatment Analysis by computing machine again.
Evenly illumination optical device is made up of ellipsoidal mirror, cold light reflector, seal glass, collimating mirror, iris, integral mirror, plane mirror, object lens, light source is assembled through ellipsoidal mirror, the plane reflection mirror reflection, after seal glass is heat insulation, become directional light by the collimating mirror converging light, iris is adjusted the size of luminous energy, become monochromatic light through the narrowband excitation optical filter, incide integral mirror, by plane mirror with object lens are oblique is mapped on the biochip, the fluorescence on the chip is caught by camera lens by the narrow emission optical filter and is imaged on the CCD camera again.
The utility model has been owing to be equipped with even illumination optical device, makes that the light intensity uniformity on the biochip has reached ± 3%, improved the accuracy of fluorescence excitation widely, and the accuracy of the fluoroscopic image that obtains thus also is greatly improved.So just avoided because the inhomogeneous error message that causes of excitation source irradiation energy.
Description of drawings
Fig. 1 is a theory structure synoptic diagram of the present utility model.
Embodiment
As shown in Figure 1, the utility model is by light source xenon lamp 1, ellipsoidal mirror 2, cold light reflector 3, seal glass 4, collimating mirror 5, iris 6, narrowband excitation optical filter 7, integral mirror 8, plane mirror 9, object lens 10, narrow emission optical filter 11, camera lens 12 and CCD camera 13 are formed, the white light that sends by xenon lamp 1, assemble through ellipsoidal mirror 2, plane mirror 3 reflections, seal glass 4 is heat insulation, by collimating mirror 5 this beam convergence light is become a branch of directional light, iris 6 can be adjusted the size of luminous energy, become monochromatic light through narrowband excitation optical filter 7 then, incide integral mirror 8, and integral mirror is spliced by tens (20-60 sheet or more) fly lenses, connecting method adopts building blocks dislocation mode, angle pencil of ray pockety can be decomposed into some light pencils, each light pencil all throws light on by the cola lighting theory, luminous energy by integral mirror 8 is evenly distributed, by plane mirror 9 and object lens 10 oblique being mapped on the biochip, fluorescence on the chip generates through exciting, and is caught by camera lens 12 by narrow emission optical filter 11 to be imaged on the CCD camera 13.
Above-mentioned ellipsoidal mirror 2 bifocals distance is 361 ± 3mm, reflect visible light, and reflectivity is greater than 96%, transmitted infrared light, transmissivity is greater than 80%; Camera lens 12 focal lengths are 50mm, and numerical aperture is 0.95.
Claims (6)
1, the CCD biochip supplies analysis that has uniform illumination system comprises light source, narrowband excitation optical filter, narrow emission optical filter, camera lens and CCD camera, it is characterized in that: be added with even illumination optical device between light source and narrowband excitation optical filter.
2, CCD biochip supplies analysis with uniform illumination system according to claim 1, it is characterized in that: described even illumination optical device is by ellipsoidal mirror, cold light reflector, seal glass, collimating mirror, iris, integral mirror, plane mirror, object lens are formed, light source is assembled through ellipsoidal mirror successively, the plane reflection mirror reflection, after seal glass is heat insulation, become directional light by the collimating mirror converging light, iris is adjusted the size of luminous energy, become monochromatic light through the narrowband excitation optical filter, incide integral mirror, by plane mirror with object lens are oblique is mapped on the biochip, the fluorescence on the chip is caught by camera lens by the narrow emission optical filter and is imaged on the CCD camera again.
3, the CCD biochip supplies analysis with uniform illumination system according to claim 2 is characterized in that: described ellipsoidal mirror bifocal distance is 361 ± 3mm, reflect visible light, and reflectivity is greater than 96%, transmitted infrared light, transmissivity is greater than 80%.
4, the CCD biochip supplies analysis with uniform illumination system according to claim 2, it is characterized in that: described collimating mirror converging light becomes directional light.
5, the CCD biochip supplies analysis with uniform illumination system according to claim 2 is characterized in that: described integral mirror is for to be spliced by fly lens, and connecting method adopts building blocks dislocation mode.
6, the CCD biochip supplies analysis with uniform illumination system according to claim 1, it is characterized in that: described lens focus is 50mm, numerical aperture is 0.95.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02252864 CN2559982Y (en) | 2002-08-27 | 2002-08-27 | CCD biochip detector with uniform lighting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02252864 CN2559982Y (en) | 2002-08-27 | 2002-08-27 | CCD biochip detector with uniform lighting system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2559982Y true CN2559982Y (en) | 2003-07-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02252864 Expired - Fee Related CN2559982Y (en) | 2002-08-27 | 2002-08-27 | CCD biochip detector with uniform lighting system |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100451630C (en) * | 2004-11-12 | 2009-01-14 | 中国科学院光电技术研究所 | biochip reading system |
| CN103604786A (en) * | 2009-03-03 | 2014-02-26 | 万迈医疗仪器有限公司 | Detection system and method for high sensitivity fluorescent assays |
| CN105424668A (en) * | 2015-12-22 | 2016-03-23 | 深圳先进技术研究院 | Fluorescent screening system |
| CN106153010A (en) * | 2016-07-25 | 2016-11-23 | 上海交通大学 | Anti-sunshine vision detection system and method based on LED and reflex reflection |
| CN106382986A (en) * | 2016-10-18 | 2017-02-08 | 中南民族大学 | Compound eye bionic micro lens light source for spectrum detection |
| CN107219207A (en) * | 2017-07-04 | 2017-09-29 | 福州大学 | A kind of automatic focusing method of CCD fluorescence biosensor chips scanner |
-
2002
- 2002-08-27 CN CN 02252864 patent/CN2559982Y/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100451630C (en) * | 2004-11-12 | 2009-01-14 | 中国科学院光电技术研究所 | biochip reading system |
| CN103604786A (en) * | 2009-03-03 | 2014-02-26 | 万迈医疗仪器有限公司 | Detection system and method for high sensitivity fluorescent assays |
| US9434789B2 (en) | 2009-03-03 | 2016-09-06 | Access Medical Systems, Ltd. | Crosslinked polysaccharide |
| US10379116B2 (en) | 2009-03-03 | 2019-08-13 | Access Medical Systems, Ltd. | Detection system for high sensitivity fluorescent assays |
| CN105424668A (en) * | 2015-12-22 | 2016-03-23 | 深圳先进技术研究院 | Fluorescent screening system |
| CN106153010A (en) * | 2016-07-25 | 2016-11-23 | 上海交通大学 | Anti-sunshine vision detection system and method based on LED and reflex reflection |
| CN106382986A (en) * | 2016-10-18 | 2017-02-08 | 中南民族大学 | Compound eye bionic micro lens light source for spectrum detection |
| CN107219207A (en) * | 2017-07-04 | 2017-09-29 | 福州大学 | A kind of automatic focusing method of CCD fluorescence biosensor chips scanner |
| CN107219207B (en) * | 2017-07-04 | 2023-10-20 | 福州大学 | Automatic focusing method of CCD biochip fluorescence scanner |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |