CN219161986U - Light source scanning type gel imaging system - Google Patents
Light source scanning type gel imaging system Download PDFInfo
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- CN219161986U CN219161986U CN202320114988.9U CN202320114988U CN219161986U CN 219161986 U CN219161986 U CN 219161986U CN 202320114988 U CN202320114988 U CN 202320114988U CN 219161986 U CN219161986 U CN 219161986U
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Abstract
The utility model discloses a light source scanning type gel imaging system, which comprises: the surface of the objective table is provided with a light-transmitting window which is positioned above the accommodating cavity and used for placing a sample; the light source assembly is arranged in the accommodating cavity and along the short side of the light transmission window and used for irradiating the sample on the light transmission window, and the light source assembly comprises at least three rows of light source modules which are arranged front and back and used for respectively emitting blue light, ultraviolet light and white light; the driving device is used for driving the light source assembly to move along the long side of the light-transmitting window; and the camera is arranged above the light-transmitting window and is used for carrying out continuous exposure imaging when the light source assembly moves to scan and irradiate. The light source scanning type gel imaging system provided by the utility model takes the requirements of various different light sources into consideration, does not need any manual operation, and automatically scans and images, so that the system is more convenient and safer to use.
Description
Technical Field
The utility model belongs to the technical field of optical imaging, and particularly relates to a light source scanning type gel imaging system.
Background
Fluorescence detection is an important method of molecular biology research. Fluorescent dyes emit light of a specific wavelength when excited by irradiation with a light source such as visible light or ultraviolet light. Therefore, in the research of molecular biology, the fluorescent dye is combined with a detection sample, then the fluorescent dye is irradiated by using a corresponding excitation light source to excite, and qualitative and quantitative analysis of the sample is realized by detecting the light intensity of the emission wavelength emitted by the fluorescent dye.
Depending on the fluorescent probe and detection method required for different samples in fluorescent detection, the commonly used excitation light sources can be roughly divided into two types, namely an ultraviolet light source and a blue light source for nucleic acid detection, and a white light source for protein-silver staining, protein-coomassie brilliant blue and colony counting, wherein the former is generally composed of an LED or fluorescent lamp tube and a filter plate, and the latter is assembled by an LED and a light homogenizing plate or converted by other light sources.
Related products in the market at present are roughly classified into three types, namely, only one ultraviolet or blue light source is provided, then the ultraviolet or blue light source is converted into a white light source through a white light conversion plate, so that ultraviolet or blue light and white light double light sources are formed, the defects are that only two light sources can be realized, and the imaging effect of the white light source is poorer than that of a real white light source because the white light source is converted through other light sources. The second type is to make ultraviolet light source module, blue light source module and white light source module separately, when using, what kind of light source module is needed, it is installed into the apparatus manually, the unused light source module needs to be disassembled, the operation is troublesome, customer and light source module contact too much, increase the biological reagent and infect the risk. The third type is that three LED lamps are uniformly distributed on the same circuit board, then an ultraviolet filter plate, a blue light filter plate and a white light homogenizing plate are manually replaced, and compared with the second type of light source, the third type of light source is not greatly improved, the operation is troublesome, and the same biological reagent infection risk exists.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provide a light source scanning type gel imaging system with three light sources.
In order to solve the above technical problems, the present utility model provides a light source scanning type gel imaging system, comprising:
the surface of the objective table is provided with a light-transmitting window which is positioned above the accommodating cavity and used for placing a sample;
the light source assembly is arranged in the accommodating cavity and along the short side of the light transmission window and used for irradiating the sample on the light transmission window, and the light source assembly comprises at least three rows of light source modules which are arranged front and back and used for respectively emitting blue light, ultraviolet light and white light;
the driving device is used for driving the light source assembly to move along the long side of the light-transmitting window;
and the camera is arranged above the light-transmitting window and is used for carrying out continuous exposure imaging when the light source assembly moves to scan and irradiate.
Further, each row of light source modules comprises a lamp panel arranged along the short side of the light transmission window and a plurality of LED lamp beads arranged on the lamp panel at intervals.
Further, the light source module further comprises a support for accommodating the lamp panel, and a filter plate is arranged on one side of the support corresponding to the LED lamp beads.
Further, the length of the light source module is larger than or equal to the length of the short side of the light-transmitting window.
Further, the objective table comprises a base with a containing cavity and a cover plate covered on the base, wherein a light-transmitting window for transmitting light is arranged in the middle of the cover plate.
Furthermore, the light-transmitting window is made of transparent glass or acrylic plates.
Further, the driving device comprises a screw rod, a screw rod nut and a motor, wherein the screw rod is arranged in the accommodating cavity in a rotating mode and arranged along the long edge of the light transmission window, the screw rod nut is arranged on the screw rod, the motor is used for driving the screw rod to rotate, and the light source assembly is fixed on the screw rod nut.
Further, a lens is arranged at the lower end of the camera.
Further, an optical filter or an optical filter wheel with a plurality of optical filters with different wavelengths is arranged below the lens.
Further, the camera is an area-array camera or a linear-array camera.
The utility model has the following beneficial effects:
in the utility model, the light source components with three different color light source modules are arranged in the object stage so as to realize the requirements of various detection, and the light source components formed by the three light source modules are driven by the driving device to move so as to form a scanning type irradiation light source, so that the detection of different samples is realized by only starting the corresponding light source module to carry out moving scanning irradiation, and the automatic scanning imaging is matched with a camera above, so that the use is more convenient and safe, the color of the three light source modules is changed without dismantling, replacing or adding an optical filter, any manual operation is not needed, the whole structure is simple, the operation is convenient, the scanning type light source is a linear light source, and the cost of the light source is greatly reduced compared with the surface light source for the whole surface irradiation in the prior art.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.
Drawings
The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this application, illustrate and do not limit the utility model, and together with the description serve to explain the principle of the utility model:
FIG. 1 is a schematic diagram of a light source scanning gel imaging system according to an embodiment;
FIG. 2 is a schematic diagram of a light source assembly of a light source scanning gel imaging system according to an embodiment;
FIG. 3 is a schematic diagram of a light source module according to an embodiment;
fig. 4 is a schematic view of a lamp panel according to an embodiment.
Detailed Description
For a more complete understanding of the present utility model, reference should be made to the following descriptions and illustrations of the present utility model in conjunction with the accompanying drawings and the detailed description thereof; it should be noted that, the text has descriptions such as "first" and "second" for distinguishing different components, and the like, and does not represent a sequence, and does not limit that "first" and "second" are different types.
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model; all other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to fall within the scope of the utility model.
Examples
As shown in fig. 1 to 4, the light source scanning gel imaging system in this embodiment includes an objective table, a light source assembly, a driving device and a camera 11, a cavity 8 is provided in the objective table, and a light-transmitting window 1 above the cavity 8 for placing a sample 7 to be detected is provided on the surface of the objective table, so that light can be conveniently irradiated on the sample to be detected through the light-transmitting window; the light source assembly is arranged in the containing cavity 8 and along the short side of the light transmission window 1, so that the light source assembly can cover and irradiate the short side of the whole light transmission window 1 to irradiate the sample to be detected on the light transmission window 1 from bottom to top so as to excite the fluorescent probes on the sample to be detected, and comprises three rows of light source modules 6 which are arranged front and back and are used for respectively emitting blue light, ultraviolet light and white light, namely the light source assembly can respectively emit the blue light, the ultraviolet light and the white light, and the light source module of which light is adopted can be selected according to the excitation light required by the fluorescent probes in the sample to be detected; the driving device is used for driving the light source assembly to move along the long side of the light-transmitting window 1, so that the light source assembly scans and irradiates along the long side of the light-transmitting window 1 to form a scanning light source, and the whole sample to be detected on the light-transmitting window 1 can be scanned; the camera 1 is arranged right above the light-transmitting window 1 and is used for carrying out continuous exposure imaging when the light source assembly moves to scan and irradiate.
It will be appreciated that the light source module 6 may also be disposed along the long side of the light-transmitting window, and the driving device drives the light source assembly to move along the short side of the light-transmitting window, in which the moving distance is shorter, but the cost of the light source module is increased.
Preferably, as shown in fig. 4, each row of light source modules 6 includes a light panel 14 disposed along a short side of the light-transmitting window 1 and a plurality of LED light beads 15 disposed at intervals along a length of the light panel; in a specific embodiment, the colors of the LED beads on the three rows of light source modules are different according to the three required light sources, and are respectively blue light, ultraviolet light and white light, so as to form a light source assembly with three different light sources.
Preferably, as shown in fig. 3, the light source module 6 further includes a bracket 13 for accommodating the light panel 14, wherein a filter plate 12 is disposed on one side of the bracket 13 corresponding to the LED light beads 15, and the filter plate 12 is used to filter out unwanted light and only transmit the excitation light with a desired color outwards, so that the LED light beads used in the three rows of light source modules 6 may be the same, and only the filter plates used in the three rows of light source modules 6 need to be different, i.e. the wavelengths of the light sources through which the three filter plates can transmit are different, so as to respectively emit blue light, ultraviolet light and white light, thereby forming a light source assembly with three different light sources.
In this embodiment, the driving device includes a screw rod 8 rotatably disposed in the cavity 8 and disposed along a long side of the light-transmitting window 1, a screw rod nut (not shown in the figure) disposed on the screw rod 8, and a motor 5 for driving the screw rod 8 to rotate, and the light source assembly is fixed on the screw rod nut; of course, in order to realize smooth sliding and rotation avoidance of the light source assembly, guide rails (not shown) are provided on both sides of the screw shaft 8, respectively, and both ends of the light source assembly are fixed to the guide rails through sliding blocks.
In this embodiment, the moving travel of the light source assembly in the cavity covers the long side of the whole light-transmitting window, and the length of the light source module 6 is equal to or greater than the short side length of the light-transmitting window 1, so that the whole light-transmitting window can be ensured to be irradiated, the imaging and detection reliability are prevented from being affected due to incomplete irradiation, the light source assembly is suitable for samples to be detected in various sizes, and the maximum applicable size is the size of the light-transmitting window.
In this embodiment, the objective table includes a base 3 having a cavity 8 and a cover plate 2 covered on the base 3, and a light-transmitting window 1 for transmitting light is arranged in the middle of the cover plate 2; preferably, the light-transmitting window 1 is made of transparent glass or acrylic plate, so that light can pass through conveniently.
In this embodiment, the lower end of the camera 11 is provided with a lens 10, and an optical filter 9 or an optical filter wheel with a plurality of optical filters with different wavelengths is arranged below the lens 10, so that the optical filters with different wavelengths can be replaced by rotating the optical filter wheel according to different requirements.
Preferably, the camera can be an area-array camera or a linear-array camera, and the aim of scanning and imaging can be achieved; when the camera is an area-array camera, continuously exposing the area-array camera to obtain a picture in one-time moving scanning process of the light source; when the camera is a linear array camera, a group of pictures are obtained by exposing one by one in the process of moving and scanning the light source, and then an image is spliced to obtain a planar array picture similar to the image shot by the planar array camera.
When the light source scanning type gel imaging system works, a sample 3 to be detected (usually DNA glue, albumin glue and the like) is placed on a light-transmitting window of an objective table, the corresponding light source module in a light source assembly and the wavelength of a light filter below a camera are selected to be started according to the type of the sample to be detected and a fluorescent probe used, clicking shooting is carried out, the light source assembly is driven by a driving device to move from a starting point on one side to an end point on the other side (as shown in fig. 1 and 2), meanwhile, the camera continuously exposes when the light source assembly starts to move until the light source assembly moves to the end point on the other side to finish exposure, namely, in the process of scanning the light source from the starting point to the end point, the camera scans and excites the other side from the side of the sample to be detected, and finally a complete exposure image of the sample to be detected is obtained.
The foregoing has described in detail the technical solutions provided by the embodiments of the present utility model, and specific examples have been applied to illustrate the principles and implementations of the embodiments of the present utility model, where the above description of the embodiments is only suitable for helping to understand the principles of the embodiments of the present utility model; meanwhile, as for those skilled in the art, according to the embodiments of the present utility model, there are variations in the specific embodiments and the application scope, and the present description should not be construed as limiting the present utility model.
Claims (10)
1. A light source scanning gel imaging system, comprising:
the surface of the objective table is provided with a light-transmitting window which is positioned above the accommodating cavity and used for placing a sample;
the light source assembly is arranged in the accommodating cavity and along the short side of the light transmission window and used for irradiating the sample on the light transmission window, and the light source assembly comprises at least three rows of light source modules which are arranged front and back and used for respectively emitting blue light, ultraviolet light and white light;
the driving device is used for driving the light source assembly to move along the long side of the light-transmitting window;
and the camera is arranged above the light-transmitting window and is used for carrying out continuous exposure imaging when the light source assembly moves to scan and irradiate.
2. The light source scanning gel imaging system of claim 1, wherein each row of light source modules comprises a light panel disposed along a short side of the light-transmitting window and a plurality of LED light beads disposed on the light panel at intervals.
3. The light source scanning gel imaging system of claim 2, wherein the light source module further comprises a bracket for accommodating a light panel, wherein a filter plate is arranged in the bracket at a side corresponding to the LED light beads.
4. A light source scanning gel imaging system as claimed in claim 3, wherein the length of said light source module is equal to or longer than the short side length of said light transmissive window.
5. The light source scanning type gel imaging system as set forth in claim 4, wherein the object stage comprises a base with a cavity and a cover plate covered on the base, wherein the light transmission window for transmitting light is arranged in the middle of the cover plate.
6. The light source scanning gel imaging system of claim 5, wherein said light transmissive window is made of transparent glass or acrylic sheet.
7. The light source scanning type gel imaging system as set forth in claim 5, wherein the driving means comprises a screw rod rotatably provided in the cavity and provided along a long side of the light-transmitting window, a screw nut provided on the screw rod, and a motor for driving the screw rod to rotate, and the light source assembly is fixed on the screw nut.
8. A light source scanning gel imaging system as claimed in any one of claims 1 to 7, wherein a lens is provided at the lower end of the camera.
9. The light source scanning gel imaging system of claim 8, wherein a filter or a filter wheel with several filters of different wavelengths is provided under the lens.
10. The light source scanning gel imaging system of claim 9, wherein said camera is an area camera or a line camera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320114988.9U CN219161986U (en) | 2023-01-16 | 2023-01-16 | Light source scanning type gel imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320114988.9U CN219161986U (en) | 2023-01-16 | 2023-01-16 | Light source scanning type gel imaging system |
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| Publication Number | Publication Date |
|---|---|
| CN219161986U true CN219161986U (en) | 2023-06-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320114988.9U Active CN219161986U (en) | 2023-01-16 | 2023-01-16 | Light source scanning type gel imaging system |
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|---|---|
| CN (1) | CN219161986U (en) |
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2023
- 2023-01-16 CN CN202320114988.9U patent/CN219161986U/en active Active
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