CN114414545B - Fluorescent scanning detection system and method - Google Patents
Fluorescent scanning detection system and method Download PDFInfo
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- CN114414545B CN114414545B CN202210070008.XA CN202210070008A CN114414545B CN 114414545 B CN114414545 B CN 114414545B CN 202210070008 A CN202210070008 A CN 202210070008A CN 114414545 B CN114414545 B CN 114414545B
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- 238000001514 detection method Methods 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 12
- 230000003287 optical effect Effects 0.000 claims abstract description 32
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 238000003825 pressing Methods 0.000 claims description 9
- 238000009434 installation Methods 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract description 5
- 238000003384 imaging method Methods 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 3
- 238000011005 laboratory method Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 238000011179 visual inspection Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000224526 Trichomonas Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1022—Measurement of deformation of individual particles by non-optical means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a fluorescence scanning detection system and a method, wherein the fluorescence scanning detection system comprises the following components: the sample mounting assembly comprises a positioning seat and a mechanical switch, and the mechanical switch is mounted at one end of the positioning seat and used for sensing whether the sample is mounted in place or not; the sliding assembly comprises a sliding mechanism and a plurality of optical filters, and the optical filters are arranged on the sliding mechanism; and the control assembly is connected with the mechanical switch and the sliding mechanism and controls the sliding mechanism to slide according to the mechanical switch signal. The invention can automatically clamp the sample to be detected and feed back information to the controller component, and the controller component automatically controls the sample to be detected, the fluorescent filter sheet and the light source to be detected, and the detection count analysis is carried out by matching with the shooting imaging of the camera, so that the detection efficiency and the accuracy are improved.
Description
Technical Field
The invention relates to the field of fluorescence detection, in particular to a fluorescence scanning detection system and method.
Background
Microorganisms including bacteria, viruses, fungi, small protozoa and the like are a large group of organisms, the microorganisms are closely related to the life of human beings, and intensive researches on the microorganisms are very necessary, such as detection and analysis on gynecological microorganisms (such as trichomonas, white hypha, spores and the like) and CD cells, so that doctors can be helped to obtain specific in-vivo information quickly, and diseases can be treated more effectively.
At present, the detection methods of microorganisms and cells are mainly a traditional laboratory method and a visual detection method, the traditional laboratory method is used for identifying the types and the amounts of microorganisms through a visual inspection method or fluorescent markers, the visual inspection method is mainly used for judging through monitoring turbidity and color changes of suspended substances in a culture solution, and the two methods have the defects of low accuracy, low efficiency, high labor intensity and the like.
Disclosure of Invention
The invention provides a fluorescence scanning detection system and a fluorescence scanning detection method, which can solve the problems of low accuracy, low efficiency and high manual labor intensity of a traditional laboratory method and a visual detection method in microorganism and cell detection and realize the purpose of rapid and accurate detection of gynecological microorganism cells and CD cells. In order to solve the above technical problems, the present invention provides a fluorescence scanning detection system, comprising:
the sample mounting assembly comprises a positioning seat and a mechanical switch, and the mechanical switch is mounted at one end of the positioning seat and used for sensing whether the sample is mounted in place or not;
the sliding assembly comprises a sliding mechanism and a plurality of optical filters, and the optical filters are arranged on the sliding mechanism;
and the control assembly is connected with the mechanical switch and the sliding mechanism and controls the sliding mechanism to slide according to the mechanical switch signal.
Preferably, the sample mounting assembly further comprises a rotating shaft seat, a rotating shaft and a pressing piece, the rotating shaft seat and the mechanical switch are mounted at one end of the positioning seat in parallel, the middle part of the rotating shaft is mounted on the positioning seat in a rotating mode through a positioning pin, one end of the rotating shaft is mounted on the rotating shaft seat, an elastic piece is arranged in the rotating shaft seat, and when the elastic piece bears pressure, one end of the rotating shaft moves upwards to enable the other end of the rotating shaft to be pressed downwards.
Preferably, the rotating shaft comprises a first rotating shaft and a second rotating shaft, the positioning pin comprises a first positioning pin and a second positioning pin, the first rotating shaft and the second rotating shaft are respectively arranged on two sides of the mounting plate through the first positioning pin and the second positioning pin, and the other ends of the first rotating shaft and the second rotating shaft further comprise pressing pieces which are arranged on the other ends of the first rotating shaft and the second rotating shaft and used for pressing.
Preferably, the sliding component comprises a screw motor, a sliding seat, a guide rail, a connecting seat and a fluorescent seat; the fluorescent seat is provided with mounting positions of the optical filters and is arranged on the sliding seat through the connecting seat, the sliding seat is connected with the screw rod of the screw rod motor, and the sliding seat is in sliding fit with the guide rail.
Preferably, the sliding assembly further comprises a travel switch and a positioning stop block, the travel switch is installed on the sliding seat through a travel switch installation seat, the positioning stop block is installed at a travel position matched with the travel switch, and the travel switch and the screw motor are both connected with the control assembly.
Preferably, the plurality of filters include a blue filter or an ultraviolet filter.
Preferably, the sample mounting assembly is mounted on an XY motion platform, the XY motion platform is mounted on a lifting assembly, the lifting assembly is arranged on a stand column, and a seat plate is arranged below the stand column.
Preferably, the control assembly comprises a motion control assembly, a screw motor driver, a voltage converter and a light source control assembly, wherein the motion control assembly is used for controlling the XY motion platform and the lifting assembly, the screw motor driver is used for driving the screw motor, and the light source control assembly is connected with the light source assembly to control the light source.
Preferably, the fluorescence scanning analysis system further comprises an objective achromatism component, the objective achromatism component comprises an objective, an optical filter and a corner fixing seat, the optical filter is installed on the optical filter seat, the objective is fixed on the corner fixing seat through an adjusting seat, the optical filter and the optical filter seat, the corner fixing seat is installed on the upright seat plate, and the other side of the corner fixing seat is sequentially connected with a lens cone, a barrel lens and a camera.
The invention also provides a fluorescence scanning detection method, which uses the fluorescence scanning analysis system and comprises the following steps:
confirming the variety class of the sample to be detected;
inserting the sample slide to be detected into the mounting plate and pushing the sample slide to the positioning seat;
the mechanical switch senses whether the sample slide to be detected is installed in place or not and feeds an installation signal back to the control assembly;
the control component controls the sliding mechanism to slide and switch the optical filter.
Preferably, the control assembly controls the sliding mechanism to slide and switch the optical filter, and then further comprises the following steps:
the control component controls the XY motion platform and the lifting component to move, so that the sample to be detected is imaged through the objective achromatic component.
According to the invention, after the sample slide to be detected is inserted into the mounting plate, the sample slide to be detected can be automatically clamped, information is fed back to the control component, the control component controls the sample slide to be detected and the fluorescent filter disc to move to corresponding positions according to the mounting information, and simultaneously, the light source is controlled to be opened to be matched with the camera for shooting and imaging, so that manual operation is not required after the sample slide to be detected is inserted, and the sample detection efficiency and the sample detection accuracy are improved.
Drawings
FIG. 1 is a general assembly diagram of a fluorescent scanning detection system;
FIG. 2 is a block diagram of a base assembly;
FIG. 3 is a block diagram of a slide assembly;
FIG. 4 is a block diagram of a sample mounting assembly;
FIG. 5 is a block diagram of an appliance control assembly;
FIG. 6 is a block diagram of an objective achromatic component;
fig. 7 is a flow chart of a fluorescence scanning detection method in example 2.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The present embodiment provides a fluorescence scanning detection system, please refer to fig. 1-6, comprising:
a sample mounting assembly 3, wherein the sample mounting assembly 3 comprises a positioning seat 303 and a mechanical switch 304, and the mechanical switch 304 is mounted at one end of the positioning seat 303 and is used for sensing whether the sample is mounted in place or not; the mechanical switch 304 may be a micro switch, and the micro switch is installed on the positioning seat 303 through a micro switch installation seat.
A slide assembly 2 including a slide mechanism and a plurality of optical filters provided on the slide mechanism; the fluorescent filter plate is placed on the fluorescent connecting seat 205, the fluorescent connecting seat 205 is installed on the guide rail 203 through the sliding block 202, and the fluorescent connecting seat 205 drives the screw rod to do linear motion through the screw rod motor 208, so that the filter plate is moved to a proper position to be matched with the light source, and the light source is filtered.
The control assembly 7 is connected with the mechanical switch 304 and the sliding mechanism, the sliding mechanism is controlled to slide according to signals of the mechanical switch 304, signals are fed back to the control assembly 7 after the mechanical switch 304 senses that a sample glass to be detected is in place, and the control assembly 7 controls the sliding mechanism to move the filter plate to the lower portion of the light source for cooperation.
In this embodiment, the sample mounting assembly 3 further includes a rotating shaft seat 301, a rotating shaft and a pressing member, the rotating shaft seat 301 and the mechanical switch 304 are installed in parallel at one end of the positioning seat 303, the middle part of the rotating shaft is installed on the positioning seat 303 through a positioning pin in a rotating manner, one end of the rotating shaft is installed on the rotating shaft seat 301, a spring is arranged in the rotating shaft seat 301, when the spring is pressed, one end of the rotating shaft moves upwards to enable the other end of the rotating shaft to be pressed downwards, a sample slide to be detected is pressed, a standard glass sheet is adopted for the slide, and other vessels conforming to the size of the mounting plate can be adopted for replacing the slide.
In this embodiment, the rotating shafts include a first rotating shaft 305 and a second rotating shaft 309, the positioning pins include a first positioning pin 306 and a second positioning pin 308, the first rotating shaft 305 and the second rotating shaft 309 are respectively mounted on two sides of the mounting plate 302 through the first positioning pin 306 and the second positioning pin 308, and the other ends of the first rotating shaft 305 and the second rotating shaft 309 further include pressing members 307 and 310 disposed thereon for pressing.
In this embodiment, the sliding assembly 2 includes a screw motor 208, a sliding seat 204, a guide rail 203, a connection seat 201, and a fluorescent seat 205; the fluorescent seat 205 is provided with two mounting positions for optical filters, the fluorescent seat 205 is arranged on the sliding seat 204 through the connecting seat 201, the sliding seat 204 is connected with a screw rod of the screw rod motor 208, and the sliding seat 204 is in sliding fit with the guide rail 203.
In this embodiment, the sliding assembly 2 further includes a travel switch 206 and a positioning stop 209, the travel switch 206 is mounted on the slide 204 through a travel switch mounting seat 207, the positioning stop 209 is mounted at a travel position matched with the travel switch 206, the travel switch 206 and the screw motor 208 are both connected with the control assembly 7, and the sliding assembly 2 is integrally mounted on the Z sliding table 103.
In this embodiment, the plurality of optical filters include an ultraviolet filter 210 and a blue filter 211, and the optical filters are made of plastic or glass sheets and added with a coating layer to filter out light within a certain wavelength range, so as to function as a monochromator. The uv filter can only pass uv light and the blue filter can only pass blue light.
In this embodiment, sample installation component 3 installs on XY motion platform 104, XY motion platform 104 installs on lifting unit, lifting unit sets up on stand 102, set up stand bedplate 101 under the stand 102, establish four stabilizer blades 105 under the stand bedplate and be used for keeping stable and certain height, the stand is installed on stand bedplate 101, set up Z slip table 103 on the stand 101, each part is connected through hexagon socket head cap screw.
In this embodiment, the control assembly 7 includes a motion control assembly 701, a screw motor driver 703, a voltage converter 704, and a light source control assembly 705, where the motion control assembly 701 is used to control the XY motion platform 104 and the lifting assembly, the screw motor driver 703 is used to drive the screw motor 208, the light source control assembly 705 is connected to the light source assembly 4 to control the light source, the motion control assembly 701, the screw motor driver 703, and the voltage converter 704 are mounted on one side of the electric board 702, and the light source control assembly 705 is mounted on the other side of the electric board 702.
In this embodiment, the fluorescence scanning detection system further includes an objective achromatic component 6, the objective achromatic component 6 includes an objective 601, an optical filter 603 and a corner fixing seat 606, the optical filter 603 is installed on the optical filter seat 604, the objective 601 is fixed on the corner fixing seat 605 through the adjusting seat 602, the optical filter 603 and the optical filter seat 604, the corner fixing seat 605 is installed on the upright base plate 101, the other side of the corner fixing seat 605 is sequentially connected with a lens barrel 606, a barrel mirror 607 and a camera 5, and the camera 5 captures a clearer slide image through the action of the objective achromatic component 6.
Example 2
This embodiment provides a fluorescence scanning detection method, using the fluorescence scanning detection system described in embodiment 1, as shown in fig. 7, comprising the steps of:
s101, confirming the variety class of the sample to be detected;
s201, inserting a sample slide to be detected into the mounting plate 302 and pushing the sample slide to the positioning seat 303;
s301, a mechanical switch 304 senses whether a sample slide to be detected is installed in place or not, and feeds an installation signal back to a control assembly 7;
s401, the control assembly 7 controls the sliding mechanism to slide and switch the optical filter.
In this embodiment, the control unit 7 further includes the following steps after controlling the sliding mechanism to slide and switch the optical filter:
the control assembly 7 controls the XY motion platform 104 and the lifting assembly to move so that the sample to be detected is imaged by the objective achromatic assembly 6.
In this embodiment, a smear method, a slide spreading method, a tabletting method or other methods are adopted to manufacture gynecological microbial cells or CD cells into a sample slide to be detected, after the sample slide to be detected is inserted into the mounting plate 302, the sample slide to be detected is pushed to the positioning seat 303 to trigger the built-in spring of the rotating shaft seat 301 to bear pressure, the rotating shafts 305 and 309 rotate around the positioning pins 306 and 308 to downwards compress the sample slide under the action of the built-in spring of the rotating shaft seat 301, meanwhile, the mechanical switch 304 senses whether the sample slide to be detected is mounted in place or not, and feeds back mounting signals to the control assembly 7, the control assembly 7 controls the travel switch 206 and the screw motor 208 to start to drive the sliding seat 204 to slide on the sliding rail 203, so that the optical filter is transported to the lower part of the light source to be matched with the light source for use, the control assembly 7 also controls the XY motion platform 104 and the lifting assembly to move the sample to the lower part of the light source, and the camera 5 photographs images of the sample slide to be detected through the objective achromatic counting assembly 6, and the images are transmitted to the computer for analysis and treatment, so that gynecological microbial cells or CD cells and the biological detection are realized.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A fluorescence scanning detection system, comprising:
the sample mounting assembly comprises a positioning seat and a mechanical switch, and the mechanical switch is mounted at one end of the positioning seat and used for sensing whether the sample is mounted in place or not;
the sliding assembly comprises a sliding mechanism and a plurality of optical filters, and the optical filters are arranged on the sliding mechanism; the sliding component comprises a screw motor, a sliding seat, a guide rail, a connecting seat and a fluorescent seat; the fluorescent seat is provided with mounting positions of the optical filters and is arranged on the sliding seat through a connecting seat, the sliding seat is connected with the screw rod of the screw rod motor, and the sliding seat is in sliding fit with the guide rail;
the sliding assembly further comprises a travel switch and a positioning stop block, the travel switch is arranged on the sliding seat through a travel switch mounting seat, the positioning stop block is arranged at a travel position matched with the travel switch, and the travel switch and the screw rod motor are both connected with the control assembly;
and the control assembly is connected with the mechanical switch and the sliding mechanism and controls the sliding mechanism to slide according to the mechanical switch signal.
2. The fluorescence scanning detection system of claim 1, wherein the sample mounting assembly further comprises a rotating shaft seat, a rotating shaft and a pressing piece, the rotating shaft seat and the mechanical switch are mounted at one end of the positioning seat in parallel, the middle part of the rotating shaft is rotatably mounted on the positioning seat through a positioning pin, one end of the rotating shaft is mounted on the rotating shaft seat, an elastic piece is arranged in the rotating shaft seat, and when the elastic piece is under pressure, one end of the rotating shaft moves upwards to enable the other end of the rotating shaft to be pressed downwards.
3. The fluorescence scanning detection system of claim 2, wherein the shaft comprises a first shaft and a second shaft, the locating pin comprises a first locating pin and a second locating pin, the first shaft and the second shaft are respectively arranged on two sides of the mounting plate through the first locating pin and the second locating pin, and a pressing piece for pressing is arranged on the other end of the first shaft and the other end of the second shaft.
4. The fluorescence scanning detection system of claim 1, wherein said plurality of filters includes a blue light filter or an ultraviolet light filter.
5. The fluorescence scanning detection system of claim 1, wherein the sample mounting assembly is mounted on an XY motion stage, the XY motion stage being mounted on a lift assembly, the lift assembly being disposed on a post, the post being provided with a seat plate.
6. The fluorescence scanning detection system of claim 5, wherein the control assembly includes a motion control assembly for controlling the XY motion stage and the lift assembly, a lead screw motor driver for driving the lead screw motor, a voltage converter, and a light source control assembly coupled to the light source assembly for controlling the light source.
7. The fluorescence scanning detection system of any one of claims 1-6, further comprising an objective achromatic assembly including an objective lens, an optical filter, and a corner mount, the optical filter being mounted on the optical filter mount, the objective lens being secured to the corner mount by an adjustment mount and the optical filter, the corner mount being mounted on the upright mount, the other side of the corner mount being sequentially connected to a lens barrel, a barrel lens, and a camera.
8. A method of fluorescence scanning detection, characterized in that a fluorescence scanning detection system according to any one of claims 1-7 is used, comprising the steps of:
confirming the variety class of the sample to be detected;
inserting the sample slide to be detected into the mounting plate and pushing the sample slide to the positioning seat;
the mechanical switch senses whether the sample slide to be detected is installed in place or not and feeds an installation signal back to the control assembly;
the control component controls the sliding mechanism to slide and switch the optical filter.
9. The fluorescence scanning assay of claim 8, wherein the control assembly further comprises the following steps after controlling the slide mechanism to slide and switch the filters:
the control component controls the XY motion platform and the lifting component to move, so that the sample to be detected is imaged through the objective achromatic component.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210070008.XA CN114414545B (en) | 2022-01-21 | 2022-01-21 | Fluorescent scanning detection system and method |
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| CN202210070008.XA CN114414545B (en) | 2022-01-21 | 2022-01-21 | Fluorescent scanning detection system and method |
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| CN116735461B (en) * | 2023-08-10 | 2023-11-10 | 成都云芯医联科技有限公司 | Three categorised optical detection systems of leucocyte |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6690470B1 (en) * | 1999-11-04 | 2004-02-10 | Arcturus Engineering, Inc. | Automated laser capture microdissection |
| CN101802675A (en) * | 2007-07-06 | 2010-08-11 | 新加坡国立大学 | Fluorescence focal modulation microscopy system and method |
| CN110057724A (en) * | 2019-05-10 | 2019-07-26 | 中国科学院苏州生物医学工程技术研究所 | Small fluorescent is inverted micro imaging system |
| CN110734854A (en) * | 2019-09-25 | 2020-01-31 | 中国科学院苏州生物医学工程技术研究所 | -body rapid detection system for real-time fluorescence quantitative analysis of ultrahigh-flux single-cell nucleic acid molecules |
| CN110799880A (en) * | 2017-08-17 | 2020-02-14 | 徕卡生物系统成像股份有限公司 | Adjustable slide holder for slides of different sizes |
| CN111272659A (en) * | 2020-03-10 | 2020-06-12 | 华东师范大学 | Full-automatic mycobacterium tuberculosis micro-scanning analyzer |
| CN111406218A (en) * | 2017-12-01 | 2020-07-10 | 徕卡生物系统成像股份有限公司 | Fixed reference edge system for slide loading and unloading |
| CN212301331U (en) * | 2020-08-21 | 2021-01-05 | 宁波舜宇仪器有限公司 | Fluorescent module mechanism |
| CN113156146A (en) * | 2021-04-12 | 2021-07-23 | 上海禹视科技有限公司 | Full-automatic high-throughput cell analysis equipment and detection method thereof |
| CN113290574A (en) * | 2021-07-12 | 2021-08-24 | 安图实验仪器(郑州)有限公司 | Clamping device for automatically grabbing sample cup |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3999662B2 (en) * | 2000-12-14 | 2007-10-31 | オリンパス株式会社 | Fluorescence analyzer and fluorescence analysis method |
| EP2344864B1 (en) * | 2008-10-24 | 2020-05-06 | Leica Biosystems Imaging, Inc. | Whole slide fluorescence scanner |
| US9229207B2 (en) * | 2008-11-17 | 2016-01-05 | Femtonics Kft | Laser scanning microscope with focus-detecting unit |
| TWI456254B (en) * | 2010-05-19 | 2014-10-11 | Ind Tech Res Inst | Fluorescence micro imaging system |
| ES2930402T3 (en) * | 2017-10-04 | 2022-12-12 | Leica Biosystems Imaging Inc | Stuck slide determination system |
-
2022
- 2022-01-21 CN CN202210070008.XA patent/CN114414545B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6690470B1 (en) * | 1999-11-04 | 2004-02-10 | Arcturus Engineering, Inc. | Automated laser capture microdissection |
| CN101802675A (en) * | 2007-07-06 | 2010-08-11 | 新加坡国立大学 | Fluorescence focal modulation microscopy system and method |
| CN110799880A (en) * | 2017-08-17 | 2020-02-14 | 徕卡生物系统成像股份有限公司 | Adjustable slide holder for slides of different sizes |
| CN111406218A (en) * | 2017-12-01 | 2020-07-10 | 徕卡生物系统成像股份有限公司 | Fixed reference edge system for slide loading and unloading |
| CN110057724A (en) * | 2019-05-10 | 2019-07-26 | 中国科学院苏州生物医学工程技术研究所 | Small fluorescent is inverted micro imaging system |
| CN110734854A (en) * | 2019-09-25 | 2020-01-31 | 中国科学院苏州生物医学工程技术研究所 | -body rapid detection system for real-time fluorescence quantitative analysis of ultrahigh-flux single-cell nucleic acid molecules |
| CN111272659A (en) * | 2020-03-10 | 2020-06-12 | 华东师范大学 | Full-automatic mycobacterium tuberculosis micro-scanning analyzer |
| CN212301331U (en) * | 2020-08-21 | 2021-01-05 | 宁波舜宇仪器有限公司 | Fluorescent module mechanism |
| CN113156146A (en) * | 2021-04-12 | 2021-07-23 | 上海禹视科技有限公司 | Full-automatic high-throughput cell analysis equipment and detection method thereof |
| CN113290574A (en) * | 2021-07-12 | 2021-08-24 | 安图实验仪器(郑州)有限公司 | Clamping device for automatically grabbing sample cup |
Non-Patent Citations (1)
| Title |
|---|
| 张刚平 等.新型快速自动宽场显微光切片控制系统.中国医学物理学杂志.2013,30(04),4322-4327. * |
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