CN216972557U

CN216972557U - Cell viewer

Info

Publication number: CN216972557U
Application number: CN202220287092.6U
Authority: CN
Inventors: 张宁博; 张猛
Original assignee: Ningbo Lixian Intelligent Technology Co ltd
Current assignee: Ningbo Lixian Intelligent Technology Co ltd
Priority date: 2022-02-11
Filing date: 2022-02-11
Publication date: 2022-07-15
Anticipated expiration: 2032-02-11

Abstract

The present invention relates to a cell viewer comprising: an incubator; the object placing table is arranged in the incubator and used for placing a culture dish; the observation mechanism comprises a lens component and a display, the lens component comprises a lens and an image sensor, the lens component is used for shooting cells in the culture dish, the display is used for displaying images, the lens component is arranged in the incubator, the display is arranged outside the incubator, and the lens component is in communication connection with the display; the first driving mechanism is connected with the lens component and is in communication connection with a processor of the cell observation instrument, and the first driving mechanism is used for driving the lens component to move along the arrangement direction of the lens component and the object placing table. The cell observation instrument can simplify the observation operation in the culture process and reduce the risk of sample pollution.

Description

Cell viewer

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell viewer.

Background

In some biochemical experiments, cell culture is an essential link. Typically, the sample is placed in a petri dish, which is placed in an incubator at a particular temperature and humidity. In each stage of the culture process, the growth state, the number and the like of cells need to be detected, the conventional method is to take out the culture dish from the incubator, place the culture dish under an observation mechanism such as a microscope and the like for observation, and place the culture dish into the incubator after the observation is finished. However, taking and placing the sample for many times not only increases the risk of sample contamination, but also has tedious operation and consumes manpower.

SUMMERY OF THE UTILITY MODEL

Based on the cell observation instrument, the utility model provides the cell observation instrument which can simplify the observation operation in the culture process and reduce the risk of sample pollution.

A cytometric instrument comprising:

an incubator;

the object placing table is arranged in the incubator and used for placing a culture dish;

the observation mechanism comprises a lens component and a display, the lens component comprises a lens and an image sensor, the lens component is used for shooting cells in the culture dish, the display is used for displaying images, the lens component is arranged in the incubator, the display is arranged outside the incubator, and the lens component is in communication connection with the display;

the first driving mechanism is connected with the lens assembly, is in communication connection with a processor of the cell observation instrument, and is used for driving the lens assembly to move along the arrangement direction of the lens assembly and the object placing table.

In one embodiment, the first driving mechanism includes a first motor and a lead screw assembly, and the first motor drives the lens assembly to move along the arrangement direction through the lead screw assembly.

In one embodiment, the lens module further comprises a frame and a movable seat, the lens module is connected with the movable seat, the object placing table is arranged above the lens module at intervals, the screw assembly comprises a screw rod and a nut, the screw rod is sleeved with the nut, the screw rod and the nut are in threaded connection, the nut is connected with the movable seat, and the movable seat is in sliding connection with the frame.

In one embodiment, one of the rack and the movable seat is connected with a sliding block, the other one of the rack and the movable seat is connected with a sliding rail, the sliding block is connected with the sliding rail in a sliding manner, and when the first motor rotates, the sliding block can slide up and down along the sliding rail.

In one embodiment, the placing table comprises a horizontally arranged placing part and a supporting part connected to the placing part, the placing part is used for placing the culture dish, the placing part is positioned above the lens, and the placing part is provided with a light through hole which is positioned right above the lens.

In one embodiment, the culture device further comprises a light source arranged in the incubator and positioned above the placing part, wherein the light source is used for illuminating the culture dish placed on the placing part.

In one embodiment, the object placing table is connected with a suspension frame extending upwards, the light source is installed on the suspension frame, and the light source is located right above the light through hole.

In one embodiment, a USB3.0 interface is disposed on the image sensor, the USB3.0 interface is used for electrically connecting with a power supply, and the USB3.0 interface is used for communicatively connecting with the processor.

In one embodiment, the incubator further comprises a second driving mechanism, the second driving mechanism is connected with the object placing table, and the object placing table can move in the vertical direction to extend out of the incubator under the driving of the second driving mechanism.

In one embodiment, the second driving mechanism includes a second motor, a gear and a rack, the gear is connected to an output shaft of the second motor, the rack extends in a vertical direction and is engaged with the gear, and the rack is connected to the object placing table.

Above-mentioned cell observation appearance, the camera lens subassembly among the observation mechanism sets up in the incubator, sets up outside the incubator with camera lens subassembly communication connection's display, consequently, need not frequently to open the incubator and take out the culture dish and observe, only need set up after the cell in the culture dish of putting the thing platform is shot through the camera lens subassembly, show through the outer display of incubator can, operating personnel can learn the cell growth state through looking over the formation of image on the display, and it is very convenient to operate. And in whole cultivation process, the culture dish is located the incubator always, and the cell can be in comparatively stable cultivation environment always, can not switch the environment and lead to the cultivation failure when putting into because of frequently taking out, also can not lead to cell pollution consequently to make the pollution risk reduce by a wide margin, cultivate the success rate and be higher. In addition, the first actuating mechanism who is connected with the lens subassembly that sets up in the incubator can drive the lens subassembly and move along the lens subassembly with the orientation of arranging of putting the thing platform to realize the focusing of camera lens, make the lens subassembly can shoot comparatively clear picture, so that operating personnel observes.

Drawings

FIG. 1 is a schematic diagram of the structure inside the incubator of the cell viewer according to an embodiment of the present invention;

fig. 2 is a schematic structural view of the components covered under the object placing table in fig. 1.

Reference numerals:

an object placing table 100, a placing part 110, a light through hole 111 and a supporting part 120;

a first motor 210, a screw rod 220 and a nut 230;

a lens 310, an image sensor 320;

the movable base 410, the supporting column 420, the connecting piece 430, the frame 440 and the extension piece 441;

a slide rail 510, a first slider 520, a second slider 530;

suspension 610, vertical extension 611, horizontal extension 612, light source 620.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 and 2, a cell observation apparatus according to an embodiment of the present invention includes an incubator, a placing table 100, an observation mechanism, and a first driving mechanism, wherein the placing table 100 is disposed in the incubator, and the placing table 100 is used for placing a culture dish. The observation mechanism comprises a lens assembly and a display, the lens assembly comprises a lens 310 and an image sensor 320, the lens assembly is arranged in the incubator, and the display is arranged outside the incubator. The lens subassembly is used for shooing the cell in the culture dish, and the display is used for showing the formation of image, lens subassembly and display communication connection. The first driving mechanism is connected with the lens assembly, is in communication connection with a processor of the cell observation instrument, is also arranged in the incubator, and is used for driving the lens assembly to move along the arrangement direction of the lens assembly and the object placing table 100.

Because the lens subassembly among the observation mechanism sets up in the incubator, sets up outside the incubator with lens subassembly communication connection's display, consequently, need not frequently to open the incubator and take out the culture dish and observe, only need set up after the cell in the culture dish of putting thing platform 100 is shot through the lens subassembly, show through the outer display of incubator can, operating personnel can learn the cell growth state through looking over the formation of image on the display, and it is very convenient to operate. And in whole cultivation process, the culture dish is located the incubator always, and the cell can be in comparatively stable cultivation environment always, can not switch the environment and lead to the cultivation failure when putting into because of frequently taking out, also can not lead to cell pollution consequently to make the pollution risk reduce by a wide margin, cultivate the success rate and be higher. In addition, the first driving mechanism connected with the lens assembly and arranged in the incubator can drive the lens assembly to move along the arrangement direction of the lens assembly and the object placing table 100, so that the focusing of the lens 310 is realized, and the lens assembly can shoot a relatively clear picture for the observation of an operator; and compared with manual focusing, the whole culture process is more convenient to operate by directly driving through the first driving mechanism.

In some embodiments, the first driving mechanism includes a first motor 210 and a lead screw assembly, and the first motor 210 drives the lens assembly to move along the arrangement direction of the lens assembly and the object placing table 100 through the lead screw assembly. Specifically, the lens assembly and the arrangement direction of the object placing table 100 are vertical directions, the lens assembly may be located below the object placing table 100, or the lens assembly may also be located above the object placing table 100, and the first motor 210 drives the lens assembly to move up and down through the screw rod assembly so as to be close to or far away from the object placing table 100. In other embodiments, the lens assembly can be directly driven by the linear motor to move up and down; alternatively, the lens assembly may be driven by an electric push rod to move up and down. Of course, other similar approaches are possible.

Specifically, in some embodiments, the mobile device further includes a frame 440 and a mobile seat 410, the lens assembly is connected to the mobile seat 410, the object stage 100 is disposed above the lens assembly at an interval, the screw assembly includes a screw 220 and a nut 230, the screw 220 is connected to an output shaft of the first motor 210, the nut 230 is sleeved on the screw 220 and is in threaded connection with the screw 220, the nut 230 is connected to the mobile seat 410, and the mobile seat 410 is slidably connected to the frame 440. Specifically, the first motor 210 is mounted on the frame 440, the bottom end of the lens 310 is fixedly connected to the image sensor 320, and a partial region of the image sensor 320 is disposed through a circular hole formed in the center of the movable base 410. The bottom plate of the image sensor 320 is fixedly connected with the bottom plate of the movable base 410 through the supporting column 420. The connecting member 430 is L-shaped, a horizontal plate extending horizontally in the connecting member 430 is sleeved outside the nut 230, and is fixedly connected to the top of the nut 230, and a vertical plate extending vertically in the connecting member 430 is fixedly connected to one side plate (the right side plate in the angle of fig. 2) of the movable base 410. The movable base 410 is slidably coupled to the frame 440, and the movable base 410 can slide up and down relative to the frame 400. When the first motor 210 rotates, the screw rod 220 connected to the output end of the first motor 210 rotates synchronously therewith, and due to the limitation of the frame 400 on the movable base 410, the movable base 410 can only move up and down synchronously with the connecting member 430 and the nut 230, so that the lens assembly moves up and down synchronously therewith. In other embodiments, a motor with an output shaft capable of being used as a lead screw is directly selected as the first motor 210, i.e., the nut is directly screwed to the output shaft of the motor.

In some embodiments, one of the frame 440 and the movable base 410 is connected with a sliding block, and the other is connected with a sliding rail, and the sliding block is slidably connected to the sliding rail, and can slide up and down along the sliding rail when the first motor 210 rotates. Through slider and slide rail cooperation, can restrict to remove seat 410 and only can reciprocate, and can lead and spacing to the removal process that removes seat 410, make its removal process more stable, the difficult position deviation that appears. Specifically, the rack 440 is connected with a slide rail 510, the movable base 410 is connected with a first slide block 520, the first slide block 520 is slidably connected to the slide rail 510, and when the first motor 210 rotates, the first slide block 520 slides up and down along the slide rail 510 to perform guiding and limiting. Alternatively, the first slider 520 and the sliding rail 510 may be interchanged. Preferably, a protruding member 441 and a second sliding block 530 are further provided, the protruding member 441 is slidably connected with the second sliding block 530, the protruding member 441 protrudes from the frame 440 and is fixedly connected with the frame, a groove is provided on the protruding member 441, the second sliding block 530 is embedded in the groove, and when the first motor 210 rotates, the second sliding block 530 slides up and down along the groove to perform guiding and limiting.

In some embodiments, the object placing table 100 includes a placing portion 110 horizontally disposed, and a supporting portion 120 connected to the placing portion 110, the placing portion 110 is used for placing a culture dish, the placing portion 110 is supported by the supporting portion 120 to be suspended above the lens 310, the placing portion 110 is provided with a light passing hole 111, and the light passing hole 111 is located right above the lens 310. Specifically, the supporting portion 120 includes four vertical plates, the placing portion 110 is a horizontal plate connected to top ends of the four vertical plates, and the supporting portion 120 and the placing portion 110 enclose a cavity in which the structures shown in fig. 2 are disposed. The region of the placing section 110 where the light transmission hole 111 is provided is a place where the petri dish is placed, and the diameter of the petri dish is larger than that of the light transmission hole 111, so that the petri dish placed therein does not fall down from the light transmission hole 111. The culture dish is transparent usually, and when placing the culture dish in clear aperture 111, make the cell in the culture dish be located clear aperture 111 in the scope, then the cell will fall into the observation scope that is located the lens subassembly that clear aperture 111 is directly under to the realization is observed. Preferably, the placing portion 110 is provided with a placing groove recessed downward, the placing groove is arranged around the light-transmitting hole 111, the shape and size of the placing groove are matched with the culture dish, the culture dish can be placed in the placing groove, the culture dish is limited by the placing groove, and therefore the culture dish is prevented from being displaced to a position far away from the light-transmitting hole 111 and being incapable of observing when the incubator is collided.

In some embodiments, the incubator further comprises a light source 620 disposed in the incubator, the light source 620 is located above the placing portion 110, and the light source 620 is used for illuminating a culture dish placed on the placing portion 110. Specifically, the light source 620 may be an LED lamp, and the culture dish may be illuminated by the LED lamp so as to observe a clearer image.

Specifically, in some embodiments, the object placing table 100 is connected with an upwardly extending suspension 610, the light source 620 is installed on the suspension 610, and the light source 620 is located right above the light passing hole 111. Specifically, the suspension 610 includes a vertical extension 611 and a horizontal extension 612. The vertical extension 611 is a vertical plate extending in the vertical direction, and the bottom end of the vertical extension 611 is connected to the placement portion 110 and the top end is connected to the horizontal extension 612. The bottom of the free end of the horizontal extension part 612 far away from the vertical extension part 611 is provided with a light source 620, and light emitted by the light source 620 is downward vertically, so that the culture dish at the light through hole 111 arranged right below the light source 620 is illuminated. In other embodiments, the suspension 610 may be independent of the stand 100.

In some embodiments, the image sensor 320 is provided with a USB3.0 interface, the USB3.0 interface is used for electrically connecting with a power supply, and the USB3.0 interface is used for communicatively connecting with the processor. Specifically, a connecting wire is inserted into the USB3.0, the other end of the connecting wire is connected to the processor, the processor is electrically connected to the power supply, and the display is in communication connection with the processor. The power supply electrically connected to the processor supplies power to the lens assembly and other components, so that the lens assembly can work normally, and meanwhile, the image shot by the image sensor 320 can be transmitted back to the processor through the connecting wire after being amplified by the lens 310 and transmitted to the display for displaying through the processor. The processor can be a computer host, and the display is a computer display screen.

In some embodiments, the incubator further comprises a second driving mechanism, the second driving mechanism is connected to the object placing table 100, and the object placing table 100 can move in the vertical direction under the driving of the second driving mechanism to extend out of the incubator. Specifically, when putting into the culture dish or cultivateing and accomplish and take out the culture dish, can put thing platform 100 through the drive of second actuating mechanism and move upwards and stretch out outside the incubator to in operating personnel gets and puts the culture dish.

In some embodiments, the second driving mechanism includes a second motor, a gear connected to an output shaft of the second motor, and a rack extending in a vertical direction and engaged with the gear, the rack being connected to the object table 100. Specifically, the second motor may also be mounted to the frame 440, and when the second motor drives the object stage 100 to move upward, the suspension 610 and the light source 620 mounted on the object stage 100 also move upward. In other embodiments, the second driving mechanism may also adopt a structure similar to the first driving mechanism, i.e. the motor is matched with the screw rod assembly to realize driving, or, the second driving mechanism may also directly adopt a driving member such as a linear motor, an air cylinder or an electric push rod to drive,

all possible combinations of the technical features of the above embodiments may not be described for the sake of brevity, but should be considered as within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A cytometric instrument, comprising:

an incubator;

2. The cytoscope of claim 1, wherein the first driving mechanism comprises a first motor and a lead screw assembly, and the first motor drives the lens assembly to move along the arrangement direction through the lead screw assembly.

3. The cell viewer of claim 2, further comprising a frame and a movable base, wherein the lens assembly is connected to the movable base, the object holder is spaced above the lens assembly, the screw assembly comprises a screw rod and a nut, the nut is disposed on the screw rod and threadedly engaged with the screw rod, the nut is connected to the movable base, and the movable base is slidably connected to the frame.

4. The cell viewer of claim 3, wherein one of the frame and the movable base is connected to a slide block, and the other of the frame and the movable base is connected to a slide rail, the slide block being slidably connected to the slide rail, and the slide block being capable of sliding up and down along the slide rail when the first motor rotates.

5. The cytoscope of claim 1, wherein the platform comprises a horizontally disposed placing portion and a supporting portion connected to the placing portion, the placing portion is used for placing the culture dish, the placing portion is located above the lens assembly, the placing portion is provided with a light hole, and the light hole is located right above the lens.

6. The cytoscope of claim 5, further comprising a light source disposed within the incubator above the placement portion, the light source for illuminating the culture dish placed on the placement portion.

7. The cell viewer of claim 6, wherein the platform is connected to a suspension extending upward, the light source is mounted to the suspension, and the light source is located directly above the light hole.

8. The cytoscope of claim 1, wherein the image sensor has a USB3.0 interface, the USB3.0 interface is configured to electrically connect to a power source, and the USB3.0 interface is configured to communicate with the processor.

9. The cell viewer of claim 1, further comprising a second driving mechanism coupled to the stage, wherein the stage is vertically movable to extend out of the incubator by the second driving mechanism.

10. The cell viewer of claim 9, wherein the second drive mechanism includes a second motor, a gear coupled to an output shaft of the second motor, and a rack coupled to the stage, the rack extending in a vertical direction and engaging the gear.