CN219348576U

CN219348576U - Cell imaging counter

Info

Publication number: CN219348576U
Application number: CN202222889495.0U
Authority: CN
Inventors: 蔡应辉; 张宏建; 秦禾; 刘晓昆
Original assignee: Gaogao Beijing Biotechnology Co ltd
Current assignee: Gaogao Beijing Biotechnology Co ltd
Priority date: 2022-10-31
Filing date: 2022-10-31
Publication date: 2023-07-14
Anticipated expiration: 2032-10-31

Abstract

The utility model discloses a cell imaging counter, which comprises: the sample stage and the bottom plate support are fixed, and the bottom plate support is provided with a microscope objective, a barrel lens, a reflecting mirror, an optical filter, a cmos camera and a fluorescence excitation light source; the sample table is also provided with a bright field light source which is coaxial with the microscope objective, and the outer cover of the bright field light source is provided with a lampshade; the fixed sample platform is provided with a groove, and the sample cell container is arranged in the groove and then fixed on the sample platform; the bottom plate support is connected with a first driving motor, and the platform is driven to drive the microscope lens, the cylindrical lens, the cmos camera, the fluorescence excitation light source and the bright field light source to synchronously move so as to photograph samples in the small sample cell container. The cell sample can be prevented from shaking by the fixed sample table, so that the photographing definition is ensured; and the moving optical photographing structure can meet the requirement of high-flux detection of multiple samples.

Description

Cell imaging counter

Technical Field

The utility model relates to the field of biological imaging optical microscopes, in particular to a novel cell imaging counter.

Background

Cells are the most basic research objects in basic scientific research, medical clinic, pharmacy and other industries, and counting, size detection and activity analysis of cells almost become indispensable works. The different cell samples are different in size and different in culture dish containers, and how to count the cell samples rapidly and accurately and the samples in different culture dish containers are problems to be solved.

At present, most image cytometry in the market detects a plurality of samples by moving a sample stage, namely adding a cell sample into a disposable cell counting plate, fixing the cell counting plate on the sample stage, moving the sample by moving the sample stage for photographing and analyzing, and easily shaking the cell sample in the moving process of the cell sample, so that photographing and imaging are not facilitated. Meanwhile, the cell counter adopting the cell counting plate mode is also difficult to detect other cell culture containers, such as cell culture pore plates (e.g. 96/48/24/12/6 pore plates), 30mm culture dishes, 90mm culture dishes, cell culture flasks, tissue culture-like chips and the like.

Disclosure of Invention

Aiming at the problems in the prior art, the utility model provides a cell imaging counter which can ensure that a cell sample does not shake, is more suitable for rapid photographing detection, and can realize rapid and accurate detection of samples in different cell containers by replacing a cell sample container through replacing an adapter.

The utility model provides a cell imaging counter, comprising: the sample stage and the bottom plate support are fixed, and the bottom plate support is provided with a microscope objective, a barrel lens, a reflecting mirror, an optical filter cmos camera and a fluorescence excitation light source; the sample table is also provided with a bright field light source which is coaxial with the microscope objective, and a light source lampshade for fluorescent counting is arranged outside the bright field light source; the fixed sample stage is provided with a groove, the groove of the sample stage can be provided with adapters of different types, a sample cell container or a multichannel cell counting sample groove is arranged on the adapter, and then the adapter is arranged in or on the groove and further fixed on the sample stage;

the bottom plate support is connected with a first driving motor, and the platform is driven to drive the microscope lens, the cylindrical lens, the cmos camera, the fluorescence excitation light source and the bright field light source to synchronously move so as to photograph samples in the sample cell container or the multichannel cell counting sample tank.

Optionally, the adapter is fixedly mounted in or above the groove by a locating pin,

optionally, the left and right sides of the light source lampshade near the sample end are provided with widened structures so as to at least cover most or all sample detection channels, and a dark field environment is mainly provided for fluorescent counting.

Optionally, the device further comprises a bright field light source cantilever structure connected with the light source lampshade, wherein the bright field light source cantilever structure is indirectly connected with the bottom plate support and used for keeping the bright field light source and the microscope objective to synchronously move and be coaxial.

Optionally, the light source lampshade outside the bright field light source can move up and down in the bright field light source cantilever structure to adjust the height.

Optionally, the microscope objective adopts objective lens groups with different multiples, and the lenses with different multiplying powers can be switched according to the requirement through a second driving motor.

Optionally, the microscope objective adopts a third driving motor to perform automatic focusing so as to realize rapid focusing and clear photographing of the sample picture.

Optionally, the horizontal base of the microscope objective is square or circular.

The cell imaging counter can ensure that a cell sample does not shake through the fixed sample table, so that the definition of photographing is ensured; the moving optical photographing structure can meet the requirement of high-flux detection of multiple samples; the replaceable sample adapter mode can be used for replacing the cell sample containers so as to realize rapid and accurate detection of samples in different cell containers; the height-adjustable bright field light source lamp or the lamp shade can realize photographing analysis of different cell containers; the sample end of the light source lampshade is widened or an independent light shielding plate is designed, so that a darkroom effect can be provided for fluorescence detection.

The technical scheme of the utility model is further described in detail through the drawings and the embodiments.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:

FIG. 1 is a front view of a cytometer in accordance with an embodiment of the present utility model, wherein the horizontal base of the microscope objective is square;

FIG. 2 is a side view of the embodiment corresponding to FIG. 1;

FIG. 3 is a cross-sectional view of the embodiment corresponding to FIG. 1;

FIG. 4 is a top view of the embodiment corresponding to FIG. 1;

FIG. 5 is a front view of a cytometer in accordance with another embodiment of the present utility model in which the horizontal base of the microscope objective is circular;

FIG. 6 is a side view of the embodiment corresponding to FIG. 5;

FIG. 7 is a cross-sectional view of the embodiment corresponding to FIG. 5;

FIG. 8 is a top view of the embodiment corresponding to FIG. 5;

the device comprises a 1-sample stage, a 2-groove, a 3-replaceable cell sample container adapter, a 4-light source lampshade, a 5-widening structure, a 6-lampshade height adjusting device, a 7-bright field light source cantilever structure, an 8-bottom plate support, a 9-first driving motor, a 10-fluorescence excitation light source, a 11-microscope objective, a 12-barrel mirror, a 13-reflecting mirror, a 14-optical filter, a 15-second driving motor, a 16-third driving motor and a 17-cmos camera.

Detailed Description

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

The preferred embodiments of the present utility model will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present utility model only, and are not intended to limit the present utility model.

As shown in fig. 1 to 8, the cell imaging counter of the present embodiment may include: the sample stage 1 and the bottom plate support 8 are fixed, and the bottom plate support 8 is provided with a microscope objective 11, a barrel lens 12, a reflecting mirror 13, a light filter 14cmos camera 17 and a fluorescence excitation light source 10. The horizontal base of the microscope objective 11 is square or circular. Fig. 1 to 4 are schematic diagrams of a cell imaging counter of a microscope objective 11 with a square horizontal base, and fig. 5 to 8 are schematic diagrams of a cell imaging counter of a microscope objective 11 with a round horizontal base.

In this embodiment, the bottom plate support 8 is connected with the first driving motor 9, the fluorescence excitation light source 10, the microscope objective 11, the cylindrical lens 12, the reflecting mirror 13, the optical filter 14 and the com camera are directly or indirectly connected and fixed on the bottom plate support 8, the bright field light source is indirectly connected to the bottom plate support 8 through the cantilever structure, the bottom plate support 8 is connected to the first driving motor 9, the bottom plate support 8 is driven by the first driving motor 9, and the microscope objective 11 lens, the cylindrical lens 12, the cmos camera 17, the fluorescence excitation light source 10 and the bright field light source can be driven to synchronously move, so that the synchronous movement of the optical components under the driving of the first driving motor 9 can be ensured, so that the sample in the small sample cell container can be photographed.

The microscope objective 11 adopts objective groups with different multiples, and if the microscope objective 11 with different multiples is required to be changed in the detection process, the lenses with different multiplying powers can be switched according to the requirement through the second driving motor 15. When the auto-focusing function is needed to detect the sample, the micro objective 11 uses the third driving motor 16 to perform auto-focusing, so as to realize fast focusing and clearly photographing the sample picture. The fluorescence excitation light source 10 irradiates the sample stage 1 and the sample from below upward, and the irradiation angle may be a right angle or an oblique angle.

The stationary sample stage 1 has a recess 2, and a sample cell container is mounted in the recess 2 and is thus fixed to the sample stage 1. Further, the groove 2 of the sample stage 1 is provided with an adapter 3 corresponding to different types of sample cell containers, the sample cell containers or the multichannel cell counting sample tanks are mounted on the adapter 3, and then the adapter is mounted in the groove 2 or on the groove 2, wherein the adapter 3 can be fixedly mounted in the groove 2 or on the groove 2 through a positioning pin. In practical applications, the reusable multi-channel cell counting chip is fixed in the adapter 3, then the adapter 3 is fixed in the groove 2 through the locating pin, and then the cell sample is added into the multi-channel cell counting chip, so that the cell sample can be kept stationary. Optionally, the adaptor 3 has protrusions positioned front and back and left and right, which can help the adaptor 3 to be accurately positioned in the groove 2 on the sample stage 1 and be fastened to the sample stage 1 by a spring buckle. Different cell sample containers can be placed on the fixed sample stage 1 through different adapters 3, and when the adapters 3 are replaced, the adapters 3 can be removed by pushing the adapters 3 backwards and then pulling upwards. For large cell sample containers, such as various well plates, culture flasks, etc., the adapter 3 can be attached and then placed on the sample stage 1 or directly placed on the sample stage 1 without the adapter 3 for observation.

The sample stage 1 is further provided with a bright field light source, the bright field light source is coaxial with the microscope objective 11, the bright field light source housing is provided with a light source lampshade 4, and the left side and the right side of the light source lampshade 4, which are close to the sample end, are provided with widening structures 5 so as to at least cover part of the sample detection channels. The bright field light source irradiates the sample downwards from the upper part of the sample table 1, is coaxial with the microscope objective 11 and is directly or indirectly fixed on the bottom plate bracket 8 through the bright field light source cantilever structure 7, and moves synchronously with the microscope objective 11.

Optionally, the cell imaging counter of this embodiment further includes a bright field light source cantilever structure 7 connected to the lampshade 4, where the bright field light source cantilever structure 7 is indirectly connected to the bottom plate support 8, so as to ensure that the bright field light source and the microscope objective move synchronously and coaxially. Optionally, the bright field light source cantilever structure 7 is connected with the bright field light source. In this embodiment, the height of the bright field light source lamp or the light source lampshade 4 is adjustable, so that photographing analysis of different cell containers can be realized. Because the fluorescent counting requires dark field conditions, the width is increased at the left side and the right side of the light source lampshade 4, which is close to the sample end, and the design is widened at the position, close to the sample end, of the light source lampshade 4, so that most or even all sample detection channels can be covered, and the effect of shading light to form a dark room is achieved when the fluorescent detection counting is carried out, and the high sensitivity and the high precision of fluorescent detection are ensured. Or, a light shielding plate is independently manufactured, and the light shielding plate is covered on as many sample detection channels as possible during each fluorescence detection, so that the light shielding effect is achieved, and a dark field environment is provided for fluorescence counting.

If it is desired to detect various cell culture well plates, cell culture dishes, cell culture flasks, organ chamber slides, etc. having a higher height, it is necessary to fix these sample containers to their respective adapters 3 and then directly put them on the fixed sample stage 1, and at this time it is necessary to adjust the light source lamp housing 4 upward by the lamp housing height adjusting means 6, ensuring that there is enough space on the fixed sample stage 1 to accommodate various adapters 3.

In practical application, when the cell imaging counter of the embodiment is used for detecting a sample, the first driving motor 9 drives all parts fixed on the bottom plate support 8 to synchronously move, and each channel of the multi-channel cell counting chip is sequentially photographed, analyzed and counted. The bright field light source is fixed on the bottom plate bracket 8 in a cantilever mode, the cell sample container can be placed on the fixed sample platform 1 through different adapters 3, the height of the bright field light source lamp or the light source lamp shade 4 can be adjusted to provide bright field illumination for different cell containers, and photographing analysis of different cell containers can be realized. Since the cell sample is placed on the fixed sample stage 1, the cell sample does not move or vibrate during the detection process, and high detection accuracy can be ensured. When the fluorescence excitation light source 10 is turned on for fluorescence detection, because external light can interfere with fluorescence, the light source lampshade 4 is designed outside the bright field light source, the widening structure 5 is arranged on the light source lampshade 4 close to the sample end, the widening structure 5 and the light source lampshade 4 are close to the multi-channel cell counting chip, the distance within 1mm is kept, all or most of the cell micro-flow chip is covered as much as possible, a darkroom can be formed, and the influence of external light on fluorescence detection is blocked.

It will be apparent to those skilled in the art that various modifications and variations can be made to the present utility model without departing from the spirit or scope of the utility model. Thus, it is intended that the present utility model also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. A cell imaging counter, comprising: the sample stage and the bottom plate support are fixed, and the bottom plate support is provided with a microscope objective, a barrel lens, a reflecting mirror, an optical filter cmos camera and a fluorescence excitation light source; the sample table is also provided with a bright field light source which is coaxial with the microscope objective, and a light source lampshade for fluorescent counting is arranged outside the bright field light source; the fixed sample stage is provided with a groove, the groove of the sample stage can be provided with adapters of different types, a sample cell container or a multichannel cell counting sample groove is arranged on the adapter, and then the adapter is arranged in or on the groove and further fixed on the sample stage;

the bottom plate support is connected with a first driving motor, and the sample platform is driven to drive the microscope objective lens, the cylindrical lens, the cmos camera, the fluorescence excitation light source and the bright field light source to synchronously move so as to photograph samples in the sample cell container or the multichannel cell counting sample tank.

2. The cell imaging counter of claim 1 wherein the adapter is fixedly mounted in or on the recess by a dowel pin.

3. The cytometer of claim 1 wherein the light source housing has a widening on the left and right sides near the sample end to cover at least a majority or all of the sample detection channel.

4. The cell imaging counter of claim 1, wherein a light shield is further provided around the brightfield light source to provide a dark field environment primarily for fluorescent counting.

5. The cell imaging counter of claim 1, further comprising a bright field light source cantilever structure coupled to the light source housing, the bright field light source cantilever structure being indirectly coupled to the base plate support for maintaining the bright field light source in synchronous motion and coaxial with the microscope objective.

6. The cytometer of claim 5 wherein the light source housing external to the bright field light source is movable up and down in the bright field light source cantilever structure to adjust the height.

7. The cell imaging counter of claim 1, wherein the microscope objective employs objective lens groups of different magnification, and the lens groups of different magnification can be switched as required by the second drive motor.

8. The cell imaging counter of claim 1 wherein the microscope objective is auto-focused using a third drive motor to achieve rapid focusing of the clearly photographed sample picture.

9. The cell imaging counter of claim 1, wherein the horizontal base of the microscope objective is square or circular.