CN214409434U - Microscopic imaging device and microscopic imaging system - Google Patents

Abstract

The utility model relates to a microscopic imaging device technical field discloses a microscopic imaging device and microscopic imaging system. The microscopic imaging device comprises an object stage, a light source, a lens unit and a camera unit. The stage is used for placing a sample to be observed. The light source is arranged on one side of the object stage and is spaced from the sample. The lens unit comprises a lens and a lens driving piece, the lens is arranged on one side, back to the light source, of the objective table and is arranged opposite to the sample, and the lens driving piece is used for driving the lens to move along the light path. The camera unit comprises a camera and a camera driving part, the camera is arranged on one side of the lens, which is back to the objective table, and the camera driving part is used for driving the camera to move along the light path. The light path of the light emitted by the light source sequentially passes through the sample and the lens of the lens and reaches the camera. The utility model discloses simplify micro-imaging device's structure, improved the practicality and the portability of device, enlarged micro-imaging device's application scope.

Description

Microscopic imaging device and microscopic imaging system

Technical Field

The utility model relates to a microscopic imaging device technical field discloses a microscopic imaging device and microscopic imaging system.

Background

In the fields of biology, chemistry and the like, microscopes are widely used, and the microscopes are used for magnifying and imaging samples through objective lenses, so that operators can conveniently observe the samples. When observing with a microscope, cases are often encountered in which the same specimen needs to be observed at varying magnifications. The variable-magnification lens can be adopted in the existing microscope to achieve the effect of changing the magnification, but the variable-magnification lens needs to change the structure of the objective lens, when the variable-magnification lens is adopted for zooming, zooming is achieved by moving the zoom group and the compensation group, the operation is complex, the selection of the appropriate magnification ratio needs to be tried for multiple times, the time is long, the zoom stability is poor, the price is high, the structure of the variable-magnification lens is complicated, and the portability and the practicability are poor.

Based on this, there is a need for a microscopic imaging apparatus and a microscopic imaging system to solve the above-mentioned problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a micro-imaging device has simplified micro-imaging device's structure, has improved the practicality and the portability of device.

To achieve the purpose, the utility model adopts the following technical proposal:

a microscopic imaging apparatus, comprising:

the objective table is used for placing a sample to be observed;

the light source is arranged on one side of the objective table and is arranged at intervals with the sample;

the lens unit comprises a lens and a lens driving piece, the lens is arranged on one side, back to the light source, of the objective table and is arranged opposite to the sample, and the lens driving piece is used for driving the lens to move along a light path;

the camera unit comprises a camera and a camera driving part, the camera is arranged on one side of the lens, which is opposite to the objective table, and the camera driving part is used for driving the camera to move along an optical path; the light path of the light emitted by the light source sequentially passes through the sample and the lens of the lens and reaches the camera.

As a preferable scheme of the microscopic imaging device, the microscopic imaging device further includes a reflection unit, the reflection unit includes a reflection mirror, the reflection mirror is disposed on the light path between the lens and the camera, the reflection mirror is disposed at an angle with respect to a vertical plane, and the reflection mirror is used for changing an angle of the light path.

As a preferred scheme of the micro-imaging device, the reflection unit further includes a fixing frame and an adjusting component, the reflector is connected to the fixing frame, the fixing frame is connected to both the lens unit and the camera unit, the fixing frame is provided with an incident hole and an exit hole, the adjusting component can adjust an angle of the reflector relative to the fixing frame, and the light path can be incident to the reflector from the incident hole and exit to the camera through the exit hole by reflection of the reflector.

As a preferred scheme of the micro-imaging device, the lens unit further comprises a connecting plate, a first light through hole is formed in the connecting plate, the lens is arranged at an interval with one side of the connecting plate, and the fixing frame is fixedly connected with the other side of the connecting plate; the light path can sequentially pass through the first light through hole and the incident hole to reach the reflector after passing through the lens.

As a preferable scheme of the micro-imaging device, the camera unit further includes a light shielding box, the camera is disposed in the light shielding box, the camera driving member is fixed on the light shielding box, a second light through hole is formed in the light shielding box, the fixing frame is connected to an outer wall of the light shielding box, and the light path is reflected by the reflector and then can sequentially pass through the emergent hole and the second light through hole to reach the camera.

As a preferable scheme of the microscopic imaging device, the microscopic imaging device further includes a following component, the lens unit is fixedly connected with the following component, and the following component can drive the lens unit, the camera unit and the reflection unit to simultaneously approach or depart from the sample.

As a preferred scheme of the micro-imaging device, the follow-up assembly includes a follow-up connecting plate and a guide rod, the guide rod is fixedly disposed and penetrates through the follow-up connecting plate, the follow-up connecting plate can slide along the guide rod, and the lens unit is fixed on the follow-up connecting plate.

As a preferred scheme of the microscopic imaging device, the microscopic imaging device further comprises a frame, the object stage, the lens unit and the camera unit are all arranged on the frame, a light source support is further arranged on the frame, and the light source is fixed on the light source support and is arranged on one side of the object stage.

As a preferable mode of the microscopic imaging apparatus, the microscopic imaging apparatus further includes a first stage driving member for driving the stage to move perpendicularly to the optical path.

Another object of the present invention is to provide a microscopic imaging system, which simplifies the structure of the microscopic imaging system and improves the practicability and portability.

To achieve the purpose, the utility model adopts the following technical proposal:

a microscopic imaging system comprising a microscopic imaging apparatus as described above.

The utility model has the advantages that: the lens is used as an objective lens, the image distance v and the object distance u can be determined when the focal length f is constant according to the magnification factor v/the object distance u, and the 1/focal length f is 1/the image distance v + 1/the object distance u, when the focal length f is constant, each selected magnification factor can determine one image distance v and one object distance u, namely, the magnification factor of the micro-imaging device can be realized by driving the lens to move along an optical path and adjusting the distance between an objective table and a camera, the magnification factor can be changed by not changing the structure of the lens, the structure of the lens is simplified, the structure of the micro-imaging device is simplified, the compactness of the structure is improved, the practicability and the portability of the device are improved, the internal structure of the micro-imaging device is also simplified, the adjusting range of the object distance u and the image distance v is expanded, and the application range of the micro-imaging device is expanded. And the object distance u and the image distance v are changed through the camera driving piece and the lens driving piece, so that the imaging definition in the camera is improved, and the operation of magnification change is simplified.

Drawings

Fig. 1 is a schematic structural diagram of a microscopic imaging apparatus provided in an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a part of a microscopic imaging device provided by an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a follower assembly, a lens unit, a camera unit and a reflection unit of a micro-imaging device according to an embodiment of the present invention;

FIG. 4 is a schematic view of the shading box shown in FIG. 3 with the shading box hidden;

fig. 5 is an exploded view of a reflection unit according to an embodiment of the present invention.

In the figure:

1. a light source; 11. a light source holder;

2. an object stage; 21. a first stage drive;

3. a lens; 31. a lens driving member; 32. a connecting plate; 321. a first light passing hole; 33. a lens holder; 331. a second chute;

4. a reflection unit; 41. a fixed mount; 411. entering a perforation hole; 412. an exit aperture; 413. a threaded hole; 42. a lens frame; 421. a bolt through hole; 422. a second accommodating groove; 423. a compression ring; 43. adjusting the bolt; 44. an elastic member; 45. a mirror;

5. a camera; 51. a camera drive; 52. a shading box; 521. a second light passing hole; 522. a first slide rail; 523. a first chute; 53. a camera mount;

61. a follow-up connecting plate; 611. a fixing plate; 612. a second slide rail; 621. a third chute; 622. a third slide rail; 63. a reset member; 64. a stopper;

7. and a frame.

Detailed Description

In order to make the technical problem solved by the present invention, the technical solutions adopted by the present invention and the technical effects achieved by the present invention clearer, the following will be described in further detail with reference to the accompanying drawings, and obviously, the described embodiments are only some embodiments of the present invention, but not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by the skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.

The present embodiment provides a microscopic imaging apparatus. Specifically, as shown in fig. 1 to 4, the microscopic imaging apparatus includes a stage 2, a light source 1, a lens unit, and a camera unit. The stage 2 is used for placing a sample to be observed. The light source 1 is disposed at one side of the stage 2 and spaced apart from the sample. The lens unit comprises a lens 3 and a lens driving part 31, the lens 3 is arranged on one side, back to the light source 1, of the object stage 2 and is opposite to the sample, and the lens driving part 31 is used for driving the lens 3 to move along a light path. The camera unit includes a camera 5 and a camera driving member 51, the camera 5 is disposed on a side of the lens 3 opposite to the stage 2, and the camera driving member 51 is configured to drive the camera 5 to move along the optical path. The light path of the light emitted by the light source 1 passes through the lens of the lens 3 and the sample in sequence and reaches the camera 5. The lens 3 is used as an objective lens, and when the focal length f is constant, one image distance v and one object distance u can be determined every time one magnification is selected, namely the magnification of the microscopic imaging device can be realized by driving the lens 3 to move along the light path and adjusting the distance between the object stage 2 and the camera 5, and the magnification can be changed by changing the structure of the lens 3 without changing the structure of the lens 3, so that the structure of the lens 3 is simplified, the structure compactness of the microscopic imaging device is improved, the practicability and the portability of the device are improved, the internal structure of the microscopic imaging device is also simplified, the adjusting range of the object distance u and the image distance v is expanded, and the application range of the microscopic imaging device is expanded. And the object distance u and the image distance v are changed through the camera driving part 51 and the lens driving part 31, so that the imaging definition in the camera 5 is improved, and the operation of magnification change is simplified. In this embodiment, a sample placing hole for placing a sample is opened on the stage 2, the sample placing hole is disposed opposite to the light source 1 and the lens 3, and light emitted from the light source 1 can reach the camera 5 through the sample placing hole, the sample and the lens 3. The structures of the lens 3 and the camera 5 are the prior art, and are not described in detail herein. And the camera 5 in the present embodiment employs a CCD camera or a CMOS camera. In other embodiments, the camera 5 may be of other types according to actual requirements, and a clamp for fixing the sample may be disposed on the stage 2, which is not limited herein.

That is, the lens driving member 31 can drive the lens 3 to move along the optical path to change the object distance u and the image distance v. It will be appreciated that the object distance u is the distance between the object plane of the sample and the lens 3, and the image distance v is the distance between the lens 3 and the light-sensitive surface of the camera 5. The distance between the object stage 2 and the camera 5 is adjustable, and the object-image distance d is adjusted by adjusting the distance between the object stage 2 and the camera 5, wherein the object-image distance d is equal to the object distance u + the image distance v, namely the object-image distance d is the distance between the object plane of the sample and the photosensitive surface of the camera 5; the magnification of the microscopic imaging apparatus is achieved by driving the lens 3 to move along the optical path and adjusting the distance between the stage 2 and the camera 5. In the present embodiment, magnification refers to optical magnification. In the prior art, the zoom range of the zoom lens is small, is usually about 4-5 times, and is difficult to meet requirements, such as incapability of obtaining finer image information of a sample, inaccurate activity detection at low magnification and the like. The microscopic imaging device provided by the embodiment has low cost and a large optical zoom range which can reach 10 times.

Specifically, as shown in fig. 1-2, the microscopic imaging apparatus further includes a gantry 7. Objective table 2, lens unit and camera unit all set up in frame 7, still are equipped with light source support 11 on frame 7, and light source 1 is fixed on light source support 11 and is placed in one side of objective table 2. In this embodiment, on rack 7's top surface was arranged in to objective table 2 level, light source support 11 was the L type, and light source support 11 includes riser and diaphragm, the bottom and the rack 7 fixed connection of riser, and the top and the diaphragm of riser are connected, and the diaphragm extends and is fixed with light source 1 towards objective table 2's top, that is to say that objective table 2's top is arranged in to light source 1, and then camera lens 3 sets up in objective table 2's below, and the light path between camera lens 3 and the light source 1 is vertical setting. The light source 1 may be an LED bulb or the like as a light emitter, and is not limited thereto. In other embodiments, the light source 1 may be disposed below the stage 2, and then the lens 3 and the camera 5 are disposed above the stage 2, which is not limited herein. The vertical plate of the light source support 11 may also be a telescopic rod structure, which can adjust the distance between the light source 1 and the sample, and the telescopic rod structure is the prior art and is not described herein again.

Further, the microscopic imaging apparatus further includes a first stage driving member 21. The first stage drive 21 is used to drive the stage 2 to move perpendicular to the optical path, facilitating observation of the sample using different fields of view. The arrangement of the first object stage driving part 21 facilitates observation of different positions of the sample, improves the practicability of the device, reduces the number of times of touching the sample by an operator, and reduces the influence of human factors on the sample. In this embodiment, parallel arrangement has two objective table spouts on objective table 2, and parallel arrangement has two objective table slide rails on frame 7, and the objective table spout all sets up along the level with the objective table slide rail, two objective table spouts and two objective table slide rails one-to-one sliding fit. First objective table driving piece 21 is first lead screw motor, and first lead screw motor fixes in frame 7, and specific first lead screw motor's output is connected in objective table 2 and can drive 2 perpendicular to light paths of objective table and remove, and first lead screw motor can drive objective table 2 and remove in the horizontal direction promptly. In other embodiments, the first stage driving member 21 may also be an air cylinder, an electric cylinder, a hydraulic cylinder, or other structures instead of the first lead screw motor, which is not limited herein. In addition, the first screw motors can be arranged into two, the output ends of the two first screw motors are arranged along the horizontal direction and are perpendicular to each other, and the range of position adjustment of the object stage 2 is enlarged. In this embodiment, the objective table 2 may be provided with a plurality of fixing holes for fixing the sample, so as to fix the sample conveniently, and the specific number and shape are determined according to the adaptability of actual requirements.

Preferably, the microscopic imaging apparatus further includes a reflection unit 4, as shown in fig. 3 to 5. The reflecting unit 4 includes a reflecting mirror 45, the reflecting mirror 45 is disposed on a light path between the lens 3 and the camera 5, the reflecting mirror 45 is disposed at an angle from the vertical plane, and the reflecting mirror 45 is used for changing an angle of the light path. The reflector 45 is arranged, so that the direction of the light path can be changed, the length size of the micro-imaging device is shortened, and the structural compactness of the micro-imaging device is improved. In the present embodiment, the reflection unit 4 can make the optical path in the vertical direction after passing through the lens 3 exit in the horizontal direction to the camera 5 after being reflected by the reflection mirror 45. In other embodiments, if the micro-imaging device has no requirement for external dimensions, the reflecting unit 4 may not be provided, and the structure is simplified.

Specifically, as shown in fig. 3 to 5, the reflection unit 4 further includes a fixing frame 41 and an adjustment assembly. The reflector 45 is connected to the fixing frame 41, the fixing frame 41 is connected to both the lens unit and the camera unit, the fixing frame 41 is provided with an incident hole 411 and an exit hole 412, the adjusting component can adjust the angle of the reflector 45 relative to the fixing frame 41, and the light path can be incident to the reflector 45 from the incident hole 411 and exit to the camera 5 through the exit hole 412 by reflection of the reflector 45. Set up the angle that adjusting part can adjust speculum 45 according to the actual conditions of light path, guaranteed that the light path can be followed and preset the direction and reachd camera 5, avoid influencing the angle of reflection light because of factors such as speculum 45's assembly error, improved the formation of image effect. In the present embodiment, the adjusting component is used to adjust the angle of the reflecting mirror 45, so as to ensure that the light path along the vertical direction can be emitted to the camera 5 along the horizontal direction after being reflected by the reflecting mirror 45.

In the embodiment, the reflecting mirror 45 is fixed on the lens frame 42, the lens frame 42 is disposed on an end surface of the fixing frame 41, and the adjusting component is connected between the lens frame 42 and the fixing frame 41. The lens frame 42 is provided with a mounting through hole, the reflector 45 is arranged in the mounting through hole, the mounting through hole is provided with a butt ring towards the upper part of the inner wall of one end of the fixing frame 41, one end of the reflector 45 is butted on the butt ring, the other end of the reflector 45 is butted against the reflector 45 through the compression ring 423, the compression ring 423 is provided with an external thread, the hole wall of the mounting hole is provided with an internal thread, and the compression ring 423 is in threaded connection with the mounting hole. The fixing frame 41 is a prism with a right-angled triangle section, the incident hole 411 is arranged on a first side wall corresponding to one of the right-angled sides, the exit hole 412 is arranged on a second side wall corresponding to the other right-angled side, the axis of the incident hole 411 and the axis of the exit hole 412 are intersected on a third side wall corresponding to the hypotenuse, and the incident hole 411 and the exit hole 412 are communicated with each other. The reflector 45 has a circular shape, and the center of the mirror surface coincides with the intersection of the axis of the entrance hole 411 and the axis of the exit hole 412. The lens frame 42 is disposed corresponding to the third sidewall, and the adjusting assembly adjusts an angle of the reflector 45 relative to the fixing frame 41 by adjusting an angle between the lens frame 42 and the third sidewall. In this embodiment, the angle between the third side wall and the vertical plane is 45 °. In other embodiments, the angle between the third sidewall and the vertical plane may be adaptively changed according to actual angle requirements, and is not limited herein.

Specifically, the adjustment assembly includes a plurality of adjustment bolts 43. The lens frame 42 is provided with a bolt through hole 421, the fixing frame 41 is provided with a threaded hole 413, and the stud of the adjusting bolt 43 penetrates through the bolt through hole 421 and is screwed in the threaded hole 413. The angle adjustment is realized by adopting the bolts, the structure is simplified, the space is saved, and the compactness of the structure is improved. In the present embodiment, three adjustment bolts 43 are provided along the circumferential direction of the reflecting mirror 45. In other embodiments, the number of the adjusting bolts 43 can also be adjusted adaptively, and is not limited herein.

Preferably, the fixing frame 41 has a first receiving groove, the lens frame 42 has a second receiving groove 422 towards the end of the fixing frame 41, and the first receiving groove and the second receiving groove 422 are opposite to each other. The reflection unit 4 further includes an elastic member 44, one end of the elastic member 44 abuts in the first accommodation groove, and the other end abuts in the second accommodation groove 422. In this embodiment, the elastic member 44 is a spring, which is easy to obtain, thereby reducing the cost and further simplifying the structure. In other embodiments, the elastic member 44 may also be a rubber ring or a torsion spring, etc., which is not limited herein. In addition, the elastic member 44 is provided in plurality along the circumference of the reflecting mirror 45, ensuring the restoring effect of the lens frame 42. In the present embodiment, four springs are provided. It can be understood that the spring is always in a compressed state, and the fixing frame 41 and the lens frame 42 are connected by the adjusting bolt 43, and two ends of the spring directly abut against the bottom surfaces of the first receiving groove and the second receiving groove 422.

Preferably, the lens driving component 31 is a second lead screw motor, the second lead screw motor can drive the lens 3 for changing the object distance u and the image distance v, the second lead screw motor is fixed on the frame 7, and an output end of the second lead screw motor is connected to the lens 3 and can drive the lens 3 to move along the optical path, that is, the second lead screw motor can drive the lens 3 to move along the vertical direction, and the length of the optical path between the lens 3 and the sample is adjusted. In other embodiments, the lens driving member 31 may also be an air cylinder, an electric cylinder, a hydraulic cylinder, or other structures instead of the second lead screw motor, which is not limited herein.

Preferably, the lens unit includes a horizontally disposed lens holder 33, the lens holder 33 is plate-shaped, one end of the lens holder 33 is provided with a lens fixing hole, the outer wall of the lens barrel of the lens 3 is fixed in the lens fixing hole, the output end of the second lead screw motor is fixed to the other end of the lens holder 33, and the lens 3 is driven by the lens holder 33 to move along the vertical direction. In other embodiments, the lens unit may further include a first displacement sensor fixed on the lens holder 33 for detecting a distance that the lens holder 33 drives the lens 3 to move, and the structure and principle of the displacement sensor are the prior art, which is not described herein again.

Preferably, as shown in fig. 4 to 5, the lens unit further includes a connection plate 32. A first light through hole 321 is formed in the connecting plate 32, the lens 3 is arranged at an interval with one side of the connecting plate 32, and the fixing frame 41 is fixedly connected with the other side of the connecting plate 32; the light path can loop through first clear aperture 321 and income perforation 411 and reach speculum 45 after camera lens 3, and reflection unit 4 passes through mount 41 and connecting plate 32 fixed connection with the camera lens unit, is convenient for fix a position reflection unit 4, has improved the inside modularization degree of device, and the assembly time has been saved to the assembly of being convenient for. In this embodiment, the connecting plate 32 extends horizontally and has one side connected to the frame 7, the other side of the connecting plate 32 is vertically provided with a first light through hole 321, the lens fixing frames 33 are disposed above the connecting plate 33 at intervals, and the lens fixing holes are opposite to the first light through hole 321. In addition, the first side wall of the fixing frame 41 is connected to the connecting plate 32 by bolts. The second screw motor is fixed at the bottom of the connecting plate 32, and the output end of the second screw motor is movably arranged on the connecting plate 32 in a penetrating manner and connected with the lens fixing frame 33.

In this embodiment, the camera driving component 51 is a third lead screw motor, the third lead screw motor can drive the camera 5 to move, and is used for changing the image distance v, specifically, an output end of the third lead screw motor is connected to the camera 5 and can drive the camera 5 to move along the optical path, the light path vertically shot to the reflecting mirror 45 through the lens 3 is shot to the camera 5 along the horizontal direction through the reflection action of the reflecting mirror 45, that is, the optical path between the camera 5 and the reflecting mirror 45 is in the horizontal direction, and the third lead screw motor can drive the camera 5 to move along the horizontal direction. In other embodiments, the camera driving member 51 may also be an air cylinder, an electric cylinder, a hydraulic cylinder, or other structures instead of the third lead screw motor, which is not limited herein.

Preferably, the microscopic imaging apparatus is further provided with a second stage drive for driving the stage 2 up or down, i.e. due to driving the stage 2 to move along the optical path. The adjustment range of the image distance v is expanded. In this embodiment, the second stage drive is a fourth lead screw motor. In other embodiments, the second stage driving element may be an air cylinder, a motor, or a hydraulic cylinder instead of the fourth screw motor, which is not limited herein. It is understood that when adjusting the image distance v, the second stage driving element and the camera driving element 51 may be activated simultaneously, or only one of the second stage driving element and the camera driving element 51 may be activated, so as to change the object image distance d. Similarly, when the object distance u and the image distance v need to be changed, the object stage 2 and the camera 5 may be fixed, and the object distance u and the image distance v may be changed by adjusting the lens 3 only, or the object distance u and the image distance v may also be changed by adjusting the object stage 2, the lens 3 and the camera 5 at the same time, which is not limited herein.

Preferably, the camera unit further includes a light shielding box 52. The camera 5 is disposed in the light shielding box 52, the camera driving member 51 is fixed on the light shielding box 52, the light shielding box 52 is provided with a second light through hole 521, the fixing frame 41 is connected to the outer wall of the light shielding box 52, and the light reflected by the reflecting mirror 45 can sequentially pass through the exit hole 412 and the second light through hole 521 to reach the camera 5. The shading box 52 is arranged, so that the camera 5 is prevented from being influenced by other light rays, the imaging quality is improved, and the functionality of the device is ensured. In the present embodiment, the light shielding box 52 has a rectangular shape, and the second light passing hole 521 is opened in the horizontal direction. Further, the camera unit includes a plate-like camera mount 53, and the camera 5 is fixed to the camera mount 53. The third screw motor is fixed on the outer wall of the light shielding box 52, and the output end of the third screw motor movably penetrates through the light shielding box 52 and is connected with the camera fixing frame 53, and drives the camera 5 to move through the camera fixing frame 53. In this embodiment, the light shielding box 52 is provided with a first slide rail 522, the first slide rail 522 is disposed along the horizontal direction, the camera fixing frame 53 is provided with a first slide groove 523, and the first slide groove 523 is in sliding fit with the first slide rail 522. In other embodiments, the camera unit may further include a second displacement sensor fixed on the camera fixing frame 53 or inside the light shielding box 52 for detecting a distance that the camera fixing frame 53 drives the camera 5 to move, and the structure and principle of the displacement sensor are the prior art, which is not described herein again. In other embodiments, other structures capable of performing a guiding function may be disposed between the camera fixing frame 53 and the light shielding box 52, and are not limited herein.

It is understood that the second sidewall of the fixing frame 41 is connected to the light shielding box 52, and the exit hole 412 is disposed opposite to the second light passing hole 521. The reflection unit 4 and the camera unit are fixedly connected with the shading box 52 through the fixing frame 41, so that the camera unit is convenient to position, the modularization degree of the interior of the device is improved, the assembly is convenient, and the assembly time is saved. In this embodiment, the lens unit, the reflection unit 4 and the camera unit are sequentially connected, so that the structure is compact, the modularization degree is high, the installation position is convenient to determine, the assembly time is shortened, and the cost is reduced.

Preferably, the microscopic imaging apparatus further comprises a follower assembly. The lens unit is fixedly connected with the follow-up assembly, and the follow-up assembly can drive the lens unit, the camera unit and the reflection unit 4 to be close to or far away from the sample at the same time. Because the year thing consumptive material that bears the sample forms of moulding plastics, the problem that realizes because of the injection moulding technology causes the bottom planarization of carrying the thing consumptive material not enough, or because the error that manual assembly technology brought for carry the best position of formation of image inconsistent of the sample under the different positions of thing consumptive material bottom, that is to say because the plane degree error, under the different positions of carrying thing consumptive material bottom, the object plane of sample and the distance between camera lens 3 are probably different, lead to the change of object distance u. Based on the above reasons, in order to ensure the observation effect, and avoid the change of the distance between the units after the distance between the units is adjusted according to the target magnification, namely, the change of the object distance u between the lens 3 and the sample is avoided, the lens unit, the camera unit and the reflection unit 4 can be simultaneously close to the sample through the follow-up assembly, or the lens unit, the camera unit and the reflection unit 4 can be simultaneously far away from the sample through the follow-up assembly, the operation of simultaneously adjusting the units is omitted, the lens unit, the camera unit and the reflection unit 4 are directly simultaneously close to or far away from the sample, and the practicability of the microscopic imaging device is improved. It is understood that, since the light source 1 in this embodiment is located above the object stage 2, the object consumables are placed on the upper surface of the object stage 2, and the object consumables may be in a shape of a sheet or a cup, which is not limited herein.

Preferably, the follow-up assembly includes a follow-up connection plate 61 and a guide rod, the guide rod is fixedly disposed and penetrated through the follow-up connection plate 61, the follow-up connection plate 61 can slide along the guide rod, and the lens unit is fixed on the follow-up connection plate 61. It will be appreciated that, since the lens unit, the camera unit and the reflection unit 4 are connected in sequence, fixing the lens unit on the follower connection plate 61 enables simultaneous movement of the lens unit, the camera unit and the reflection unit 4 when moving the follower connection plate 61. In this embodiment, the blind hole has been seted up to the one end of follow-up connecting plate 61, and the guide bar is arranged in the blind hole for follow-up connecting plate 61 can slide along the guide bar. Further, the guide bar is arranged in a vertical direction and the bottom of the guide bar is connected with the frame 7, that is, the guide bar is fixedly arranged through the frame 7. Further, the connection plate 32 of the lens unit is fixedly connected to the follower connection plate 61.

In this embodiment, the bottom of the guide rod is provided with a stop member 64 and a reset member 63, the stop member 64 is convexly arranged on the guide rod, the reset member 63 is sleeved on the guide rod 61, the bottom end of the reset member 63 abuts against the stop member 64, and the top end abuts against the follow-up connecting plate 61. The stop member 64 is fixed to the bottom end of the guide rod in a block shape and is detachably connected to the frame 7. In the present embodiment, the returning member 63 is a spring. In other embodiments, the restoring member 63 may also be a rubber ring or the like, which is not limited herein.

In this embodiment, the fixed plate 611 is connected to the following connection plate 61, the fixed plate is provided with a second slide rail 612, the second slide rail 612 is disposed along the vertical direction, the lens holder 33 is provided with a second slide groove 331, and the second slide groove 331 is in sliding fit with the second slide rail 612. The second sliding groove 331 and the second sliding rail 612 are arranged, so that the moving precision of the lens 3 along the light path is improved, and the functionality of the micro-imaging device is ensured. In addition, a third sliding groove 621 is arranged on the follow-up connecting plate 61, a third sliding rail 622 is fixedly arranged on the rack 7, the third sliding rail 622 is arranged along the vertical direction, and the third sliding groove 621 is in sliding fit with the third sliding rail 622. Set up third spout 621 and third slide rail 622, guaranteed that the follow-up subassembly can only drive lens unit, camera unit and reflection unit 4 and be close to or keep away from along vertical direction, prevent to deviate the observation field of vision.

The embodiment also provides a microscopic imaging system. A microscopic imaging system includes a microscopic imaging apparatus as provided above. Preferably, the microscopic imaging system comprises a housing, a controller and a display operation screen, wherein the controller is arranged inside the housing, and the display operation screen is arranged outside the housing. The display operation screen, the first lead screw motor, the second lead screw motor, the third lead screw motor and the fourth lead screw motor are all electrically connected with the controller, an operator can input magnification through the display operation screen, the controller can control the second lead screw motor, the third lead screw motor and the fourth lead screw motor to move, the first lead screw motor can also be controlled to move through the controller, and before the microscopic imaging system is used or after the microscopic imaging system is used, the movable object stage 2 is used for placing or withdrawing a sample. The display operation screen, the first lead screw motor, the second lead screw motor, the third lead screw motor and the fourth lead screw motor can be in wired connection or wireless communication connection with the controller, and the connection mode and the structure of the controller are the prior art and are not repeated herein.

The microscopic imaging apparatus provided in the present embodiment employs the following microscopic imaging method. The microscopic imaging method comprises the following steps:

acquiring a target magnification;

adjusting the object image distance d based on the target magnification and the focal length f of the lens 3;

moving the lens 3 along the optical path to adjust the object distance u and the image distance v based on the target magnification and the focal length f of the lens 3;

the light source 1 is caused to illuminate the sample along the optical path and the camera 5 acquires a microscopic image of the sample.

The object-image distance d is driven through the target magnification, the object distance u and the image distance v are adjusted, the relative position of the lens 3 is adjusted between the sample with the determined distance and the camera 5, the adjusting times of the lens 3 can be reduced, the operation is simplified, and the practicability is improved.

It is understood that the object distance u is the distance between the object plane of the sample and the lens 3, the image distance v is the distance between the lens 3 and the photosensitive surface of the camera 5, and the object distance d is the distance between the object plane of the sample and the photosensitive surface of the camera 5.

Specifically, the target magnification ranges from 1 to 10 times. In this embodiment, the target magnification includes a first magnification and a second magnification, where the first magnification is smaller than the second magnification, a first microscopic image of the sample can be acquired with the first magnification, a second microscopic image of the sample can be acquired with the second magnification, the first microscopic image is used for determining the number and concentration of cells in the sample, and the second microscopic image is used for determining the viability of cells in the sample. If the survival rate and the concentration of the cells are calculated and analyzed under the same multiplying power, result deviation is easily caused. If the magnification is smaller, the dying and living cells are stained with unobvious differentiation, so that the judgment of the dying and living of the cells is influenced; similarly, if the cell concentration is analyzed at a high magnification, the result of the cell concentration analyzed at different fields of view may vary greatly due to artificial sample application variation, systematic variation, and the like, and the observation field at a high magnification may be small.

When the microscopic imaging device provided by the embodiment is used for detecting the cell concentration and the cell viability, an operator inputs a first magnification and a second magnification through the display operation screen, wherein the first magnification is less than the second magnification, and also inputs an experiment type corresponding to the first magnification as concentration detection and an experiment type corresponding to the second magnification as the cell viability detection through the display operation screen. Then, a first microscopic image of the sample is obtained under a first magnification, the cell concentration is detected, then a second microscopic image of the sample is obtained under a second magnification, and the cell viability is detected; the visual field is changed, the operation is repeated for a plurality of times, and the cell concentration and the survival rate are respectively calculated by taking the average value. The method may further include acquiring first microscopic images of the samples in the multiple fields of view at the same time under a first magnification, detecting the cell concentration, and averaging, and acquiring second microscopic images of the samples in the multiple fields of view at the same time under a second magnification, detecting the cell viability, and averaging, which is not limited herein.

Specifically, under different magnifications, the MTF value (contrast Transfer Function) of the lens 3 is not less than 0.15, which ensures the imaging quality.

It will be appreciated that the microimaging apparatus also includes a second stage drive for driving the stage 2 along the optical path. The microscopic imaging method further comprises: the object distance d is adjusted by moving the sample along the optical path and/or the camera 5. Specifically, the sample and the camera 5 are moved by the second stage driving unit and the camera driving unit 51, respectively.

Since the microscopic imaging device in this embodiment further includes the reflection unit 4, the reflection unit 4 includes the reflection mirror 45, the reflection mirror 45 is disposed on the light path between the lens 3 and the camera 5, the reflection mirror 45 is disposed at an angle with the vertical plane, and the reflection mirror 45 is used for changing the angle of the light path. Further, the microscopic imaging method further comprises: after light emitted by the light source 1 is irradiated to the sample along the optical path, the angle of the optical path is changed by the reflection unit 4 and reaches the camera 5.

To facilitate the determination of the object-image distance d, the following formula is used: 1/f is 1/u +1/v, m is v/u and d is u + v, the functional relation between the target magnification and the object image distance d is determined, and when the target magnification is changed, the size of the object image distance d can be obtained based on the functional relation; wherein f is the focal length of the lens 3, and m is the target magnification; v is the object distance; u is an image distance; d is the object-image distance.

To facilitate the determination of the object distance u and the image distance v, the following formula is used: 1/f is 1/u +1/v, m is v/u and d is u + v, the functional relation between the object distance u and the image distance v is determined, and when the object distance u is changed, the image distance v can be obtained based on the functional relation; f is the focal length of the lens 3, and m is the target magnification; v is the object distance; u is an image distance; d is the object-image distance.

The focal length f of the lens 3 used in this embodiment is 10.2mm, and a theoretical value can be obtained according to the above formula: when the target magnification is 4.6, the obtained object-image distance d is 69.54mm and is approximately 70mm, the object distance u is 12.5mm, and the image distance v is 57.5 mm; when the target magnification is 6.6, the obtained object-image distance d is 89.26mm and is approximately 90mm, the object distance u is 11.8mm, and the image distance v is 77.88 mm; when the target magnification is 8.6, the object distance d is 109.3mm and is approximately 110mm, the object distance u is 11.45mm, and the image distance v is 98.47 mm.

However, when a target magnification is determined, since the lens 3 is cylindrical, the central axis extends along the optical path, and in practice, it is often convenient to measure the second distance L2 between the lens of the lens 3 toward the end of the specimen and between the lens of the lens 3 toward the end of the camera 5 and the camera 5. Further, when the functional relationship between the first distance L1 and the second distance L2 of the micro-imaging device at the target magnification is tested, the functional relationship between the first distance L1 and the second distance L2 can be obtained by fitting at least two sets of data of the first distance L1 and the second distance L2, so that the micro-imaging device can be used in practice. The focal length f of the lens 3 is 10.2mm, and when the target magnification is 4.6, the first distance L1 is 8.35mm, and the second distance L2 is 52.59 mm; when the target magnification is 6.6, the first distance L1 is 7.67mm, and the second distance L2 is 73.26 mm; when the target magnification is 8.6, the first distance L1 is 7.32mm, and the second distance L2 is 93.61 mm.

Similarly, when the microscopic imaging device is tested at the target magnification, in order to obtain the functional relationship between the actual numerical value of the object image distance d and the target magnification, the functional relationship between the target magnification and the actual numerical value of the object image distance d can be obtained by fitting at least two groups of data of the target magnification and the actual numerical value of the object image distance d, which is convenient for the practical use of the microscopic imaging device.

It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. A microscopic imaging apparatus, comprising:

the objective table (2) is used for placing a sample to be observed;

the light source (1) is arranged on one side of the objective table (2) and is arranged at an interval with the sample;

the lens unit comprises a lens (3) and a lens driving piece (31), the lens (3) is arranged on one side, back to the light source (1), of the objective table (2) and is arranged opposite to the sample, and the lens driving piece (31) is used for driving the lens (3) to move along a light path;

the camera unit comprises a camera (5) and a camera driving part (51), the camera (5) is arranged on one side of the lens (3) opposite to the object stage (2), and the camera driving part (51) is used for driving the camera (5) to move along a light path; the light path of the light emitted by the light source (1) sequentially passes through the sample and the lens of the lens (3) and reaches the camera (5).

2. A microscopic imaging apparatus according to claim 1, characterized in that the microscopic imaging apparatus further comprises a reflection unit (4), the reflection unit (4) comprises a mirror (45), the mirror (45) is arranged on the light path between the lens (3) and the camera (5), the mirror (45) is arranged at an angle to the vertical, and the mirror (45) is used for changing the angle of the light path.

3. A microscopic imaging apparatus according to claim 2, wherein the reflection unit (4) further comprises a fixing frame (41) and an adjusting component, the reflection mirror (45) is connected to the fixing frame (41), the fixing frame (41) is connected to both the lens unit and the camera unit, the fixing frame (41) is provided with an entrance hole (411) and an exit hole (412), the adjusting component can adjust the angle of the reflection mirror (45) relative to the fixing frame (41), and the light path can be incident on the reflection mirror (45) from the entrance hole (411) and exit to the camera (5) through the exit hole (412) after being reflected by the reflection mirror (45).

4. The microscopic imaging device according to claim 3, wherein the lens unit further comprises a connecting plate (32), the connecting plate (32) is provided with a first light through hole (321), the lens (3) and one side of the connecting plate (32) are arranged at intervals, and the fixing frame (41) is fixedly connected with the other side of the connecting plate (32); the light path can sequentially pass through the first light through hole (321) and the incident hole (411) after passing through the lens (3) and then reach the reflector (45).

5. The microscopic imaging apparatus according to claim 4, wherein the camera unit further comprises a light shielding box (52), the camera (5) is disposed in the light shielding box (52), the camera driving member (51) is fixed on the light shielding box (52), a second light through hole (521) is formed in the light shielding box (52), the fixing frame (41) is connected to an outer wall of the light shielding box (52), and the light reflected by the reflector (45) can sequentially pass through the exit hole (412) and the second light through hole (521) to reach the camera (5).

6. A microscopic imaging apparatus according to claim 5, characterized in that said apparatus further comprises a follower assembly, said lens unit being fixedly connected to said follower assembly, said follower assembly being capable of driving said lens unit, said camera unit and said reflection unit (4) simultaneously towards or away from said sample.

7. The microscopic imaging apparatus according to claim 6, wherein the follow-up assembly comprises a follow-up connecting plate (61) and a guide rod, the guide rod is fixedly arranged and penetrated through the follow-up connecting plate (61), the follow-up connecting plate (61) can slide along the guide rod, and the lens unit is fixed on the follow-up connecting plate (61).

8. The microscopic imaging apparatus according to claim 1, further comprising a frame (7), wherein the stage (2), the lens unit and the camera unit are disposed on the frame (7), a light source bracket (11) is further disposed on the frame (7), and the light source (1) is fixed on the light source bracket (11) and disposed on one side of the stage (2).

9. A microscopic imaging apparatus according to claim 1, characterized in that the microscopic imaging apparatus further comprises a first stage drive (21), the first stage drive (21) being adapted to drive the stage (2) to move perpendicular to the optical path.

10. A microscopic imaging system comprising the microscopic imaging apparatus of any one of claims 1 to 9.

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