KR101627337B1 - Sample Chamber Cartridge for Reducing Field Curvature - Google Patents

Sample Chamber Cartridge for Reducing Field Curvature Download PDF

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KR101627337B1
KR101627337B1 KR1020140105142A KR20140105142A KR101627337B1 KR 101627337 B1 KR101627337 B1 KR 101627337B1 KR 1020140105142 A KR1020140105142 A KR 1020140105142A KR 20140105142 A KR20140105142 A KR 20140105142A KR 101627337 B1 KR101627337 B1 KR 101627337B1
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South Korea
Prior art keywords
sample chamber
sample
curvature
cartridge
imaging device
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KR1020140105142A
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Korean (ko)
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KR20160020155A (en
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김중경
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국민대학교산학협력단
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Abstract

The present invention improves the shape of a sample chamber into which a sample is injected without changing the structure of the imaging apparatus and corrects the curvature of the image due to the lens of the imaging apparatus so that accurate images can be obtained not only at the center, [0001] The present invention relates to a sample chamber cartridge, and more particularly, to a sample chamber cartridge comprising: a main body having a sample chamber therein accommodating a liquid sample; A transparent window formed on an upper surface of the body to form an upper surface of the sample chamber and to acquire an optical image of the sample; And the lower surface of the sample chamber is curved downwardly.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a sample chamber cartridge,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample chamber cartridge for analyzing a liquid sample such as blood, and more particularly, to a sample chamber cartridge for analyzing a liquid sample in a liquid sample by injecting a bio- The present invention relates to a top surface curvature correction type sample chamber cartridge capable of correcting a curvature of a surface caused by a lens of a device and obtaining an overall accurate image.

In the bio / environment field, the medical examination and environmental pollution investigation process is common to lead to sample collection - sample preparation - reagent reaction - detection and analysis, and the basic test method is to measure the concentration of particulate matter contained in the sample. Conventionally, a device for analyzing optical signals detected by irradiating a liquid flow or an air flow with a sample has been mainly developed. However, in consideration of the miniaturization, portability, convenience, and economical efficiency of devices, And photodetectors are being replaced by inexpensive LED light sources and CMOS cameras and are being used in the development of point-of-care devices.

In order to measure the particle concentration by analyzing the image of the particulate sample obtained by the camera, a sample chamber capable of holding a certain volume of sample is required. The particle concentration = (total number of particles included in the sample) / (sample volume) .

However, when measuring the particle concentration by photographing a sample with a camera, various aberrations including a field curvature aberration caused by a biconvex lens (L) of the camera as shown in Figs. 1 and 2, When the center of the image is focused, the edge portion of the image is distorted, so that the accurate particle concentration can not be measured.

Conventionally, a method of providing another lens having a different refractive index on the entire surface of a convex lens of an image pickup device (see Japanese Patent Laid-Open Publication No. 2007-0087214) or a surface of an image sensor is formed as a curved surface to correct aberrations, The configuration of the apparatus becomes complicated, and the structure of the image pickup apparatus is inevitably changed, which is disadvantageous in terms of economy.

Korean Patent No. 10-0844350 (registered on July 01, 2008) Korean Patent Publication No. 2007-0087214 (Published Date August 27, 2007)

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to improve the shape of a sample chamber into which a sample is injected without changing the structure of the imaging apparatus, In addition, the present invention provides a sample chamber cartridge capable of acquiring an accurate image even at an edge portion or an edge portion.

According to an aspect of the present invention, there is provided a sample chamber cartridge including: a body having a sample chamber therein for accommodating a liquid sample; A transparent window formed on an upper surface of the body to form an upper surface of the sample chamber and to acquire an optical image of the sample; And the lower surface of the sample chamber is curved downwardly.

According to one aspect of the present invention, the radius of curvature (r 0 ) of the lower surface of the sample chamber is expressed by the following equation

Figure 112014076672669-pat00001

Where n 0 is the refractive index of the medium between the positive lens and the specimen chamber of the image pickup device, n 1 is the refractive index of the positive lens, n 2 is the refractive index of the positive lens of the imaging device, And K 1 and K 2 are the refractive indices of the two convex lenses of the imaging device

Figure 112014076672669-pat00002
,
Figure 112014076672669-pat00003
, Wherein R 1 and R 2 are curvature radii of both surfaces of the positive lens of the image pickup device, respectively.

According to another aspect of the present invention, the lower surface of the sample chamber is formed of a thin plate of a flexible material, and the main body may be provided with variable means for varying the curvature by applying pressure to the lower surface of the sample chamber.

The variable means may include a hydraulic controller for supplying or discharging a fluid to a space formed between a lower surface of the sample chamber and a lower surface of the main body.

Or the variable means may include a plurality of contact portions formed at the edge portion of the lower surface of the sample chamber and an upper portion passing through the lower surface of the body so as to be in close contact with the contact portion and moving up and down with respect to the lower surface of the body, And a plurality of adjustment screws for adjusting the height of edge portions of the surface.

Since the sample chamber into which the sample is injected is made of a curved surface having a curvature corresponding to the curvature of both convex lenses of the image pickup apparatus of the present invention, when the image pickup apparatus captures a sample in the sample chamber, The occurrence of distortion at the edge portion of the image is suppressed, and an accurate image as a whole can be obtained. Therefore, the particle concentration of the sample can be accurately measured.

1 is a view for explaining an optical action (surface curvature phenomenon) by a conventional sample chamber cartridge.
2 is an optical photograph showing an example of an image in which an edge portion is distorted by a curvature of the surface.
FIG. 3 is a cross-sectional view illustrating the structure of a top surface curvature-correcting sample chamber cartridge according to an embodiment of the present invention.
Fig. 4 is a view corresponding to Fig. 1, illustrating the optical action of a top surface curvature-correcting sample chamber cartridge according to the present invention.
FIG. 5 is a cross-sectional view illustrating the structure of a top surface curvature-correcting sample chamber cartridge according to another embodiment of the present invention.
6 is a cross-sectional view illustrating a structure of a top surface curvature correction type sample chamber cartridge according to another embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of a top surface curvature-correcting sample chamber cartridge according to the present invention will be described in detail with reference to the accompanying drawings.

Referring to FIG. 3, a sample chamber cartridge according to an embodiment of the present invention includes a main body 10 having a sample chamber 20 accommodating a liquid sample therein, And a window 22 made of a transparent material for forming an upper surface of the sample chamber 20 and for obtaining an optical image of the sample.

The sample chamber 20 may be square or circular when viewed from above. The lower surface of the sample chamber 20 is curved downwardly so as to correct the curvature of the surface caused by the convex lenses of the imaging device (camera).

The window 22 is made of transparent glass or synthetic resin to form an upper surface of the sample chamber 20 so as to be photographed by the imaging device and is formed as a concave curved surface downward as the sample chamber 20. The radius of curvature of the window 22 is preferably equal to the radius of curvature of the lower surface 21 of the sample chamber 20.

Since the lower surface 21 of the sample chamber 20 and the window 22 are formed as curved surfaces as described above, the sample chamber 20 is formed into a curved surface having a predetermined curvature.

As shown in Fig. 1, the focal point of the light beam that has passed through both convex lenses L of the imaging device starting from the plane of the object (the existing sample chamber) is located on the curved surface, Petzval Surface), and expressed by the following Equation (1).

Figure 112014076672669-pat00004

Here, r 0 is the radius of curvature of the sample chamber, and r 2 is the radius of curvature of the surface formed by the focal point of the ray, that is, the Petzval surface. N 0 is the refractive index of the medium between the positive lens L and the sample chamber 20 of the imaging device, n 1 is the refractive index of the positive lens, and n 2 is the positive refractive power of the positive lens L of the imaging device. And the refractive index of the medium (typically air) between the image sensor of the imaging device and the image sensor. n 0 and n 2 are typically 1.0 as the refractive index of air.

And K 1 and K 2 are magnifications of the positive lens L of the imaging device

Figure 112014076672669-pat00005
,
Figure 112014076672669-pat00006
, Where R 1 and R 2 are the curvature radii of the both surfaces of the positive lens L of the imaging device, respectively.

Since the conventional sample chamber 20 is planar, 1 / r 0 = 0, so that a Petzval Surface r 2 having a curved surface is obtained.

The lower surface (21) of the sample chamber (20) of the sample chamber cartridge according to the present invention has a surface (21) formed on the image sensor of the imaging device, which is not a curved surface but a plane, (See Fig. 4).

Therefore, in the equation (1), the condition that the Petzval plane becomes a plane, that is, the radius of curvature r 0 of the object to be measured when 1 / r 2 = 0 is the radius of curvature of the sample chamber 20, Is expressed by Equation (2).

Figure 112014076672669-pat00007

Here, the medium between the biconvex lens (L) of the imaging device and the sample chamber 20 and the medium between the convex lens (L) of the imaging device and the image sensor of the imaging device are usually n 0 and n 2 is 1.0, and therefore the equation for the radius of curvature (r 0 ) of the sample chamber 20 can be expressed as follows.

Figure 112014076672669-pat00008

The lower surface 21 of the sample chamber 20 may be integrally formed or fixed to the main body 10 but may be formed so as to be capable of changing the radius of curvature of the lower surface 21 You can do it.

5, the lower surface 21 of the sample chamber 20 is formed of a thin plate of a flexible polymer material and the space 15 is formed below the lower surface 21, (Not shown) may be used to adjust the radius of curvature of the lower surface 21 by controlling the pressure inside the space 15 to a predetermined pressure by supplying air or discharging fluid such as oil or water through the space 15 There will be. The lower surface 21 of the sample chamber 20 is provided at the lower side 21 of the sample chamber 20 so that the radius of curvature of the lower surface 21 of the sample chamber 20 can be easily varied by fluid pressure. It is preferable that a slot 14 is formed to be slidably inserted.

The hydraulic controller supplies fluid to a lower portion of one side of the main body 10 through an inlet port 11 formed to communicate with the space 15 and communicates with the space 15 at a lower portion of one side of the main body 10, And regulates the fluid pressure inside the space 15 by discharging the fluid through the outlet port 12 formed as a result. The fluid introduced into the space 15 applies a predetermined pressure to the lower surface 21 of the sample chamber 20 and thus the radius of curvature of the lower surface 21 is varied.

The fluid supplied to the inside of the space 15 between the lower surface 21 of the sample chamber 20 and the lower surface of the main body 10 by the hydraulic controller moves the edge portion of the lower surface 21 of the sample chamber 20 The reinforcing ribs 23 may be formed in the center of the lower surface 21 of the sample chamber 20 so as to be deformed.

6, a plurality of contact portions 25 are formed to protrude from the edge portion of the lower surface 21 of the sample chamber 20 and the lower surface of the body 10 is attached to the body 10 A plurality of adjusting screws 30 are provided so as to be in close contact with the contact portion 25 so as to pass through the lower surface 21 of the sample chamber 20 by rotating the adjusting screw 30 to adjust the height of the edge portion of the lower surface 21 of the sample chamber 20, 21 may be adjusted.

As described above, the sample chamber cartridge of the present invention has the sample chamber 20 in which the sample is injected is formed of a curved surface having a curvature corresponding to the curvature of the convex lens L of the imaging device, The distortion of the edge portion of the image due to the curvature of the surface of the biconvex lens L is suppressed, and the entire image can be obtained as a whole. Therefore, the particle concentration of the sample can be accurately measured.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the present invention as defined by the appended claims. And it is to be understood that such modified embodiments belong to the scope of protection of the present invention defined by the appended claims.

10: main body 11: inlet port
12: exhaust port 15: space
20: sample chamber 21: lower surface
22: window L: biconvex lens

Claims (8)

A convex lens L is provided at the lower end of the positive lens L and a distance L is provided below the image pickup apparatus provided with an image sensor on the upper side of the positive lens L. A liquid sample to be imaged by the image pickup apparatus is accommodated In the sample chamber cartridge,
A body 10 in which a sample chamber 20 for containing a liquid sample is formed;
And a window (22) made of a transparent material for obtaining an optical image of the sample, the window (22) being provided on the upper surface of the body (10) to constitute the upper surface of the sample chamber (20)
The lower surface (21) of the sample chamber (20) is formed with a concave downwardly curved surface;
The radius of curvature r 0 of the lower surface 21 of the sample chamber 20 is given by the following equation
Figure 112016006619227-pat00009

But satisfies, where n 0 is the medium refractive index of between the biconvex lens (L) and a sample chamber 20 of the imaging device, n 1 is the refractive index of the biconvex lens (L) of the imaging device, n 2 is the image pickup Is the refractive index of the medium between the positive lens (L) of the device and the image sensor of the image pickup device, and K 1 and K 2 are the magnification of the positive lens (L) of the imaging device
Figure 112016006619227-pat00010
,
Figure 112016006619227-pat00011
, Wherein R 1 and R 2 are curvature radii of both surfaces of a biconvex lens (L) of the imaging device, respectively. delete The cartridge according to claim 1, wherein the window (22) is also curved downwardly. 4. The cartridge according to claim 3, wherein the window (22) has the same radius of curvature as the lower surface of the sample chamber (20). The apparatus according to claim 1, wherein a lower surface (21) of the sample chamber (20) is formed of a thin plate of a flexible material, and a pressure is applied to the lower surface (21) of the sample chamber (20) Wherein the sample chamber is provided with a variable means for varying the length of the sample chamber. 6. The apparatus according to claim 5, wherein the variable means includes a hydraulic controller for supplying or discharging a fluid into a space (15) formed between a lower surface (21) of the sample chamber (20) Wherein the sample chamber cartridge is a surface-deflection type correction chamber. 7. The cartridge according to claim 6, wherein a reinforcing rib (23) is formed in the center of the lower surface (21) of the sample chamber (20) in a positive (+) or radial direction. 6. The apparatus according to claim 5, wherein the variable means comprises: a plurality of contact portions (25) formed on edge portions of a lower surface (21) of the sample chamber (20); an upper end portion passing through a lower surface of the body And a plurality of adjusting screws 30 which are in tight contact with the lower surface 25 of the sample chamber 20 and move up and down with respect to the lower surface of the body 10 to adjust the height of the edge of the lower surface 21 of the sample chamber 20 A sample chamber cartridge for correcting a curvature of field.
KR1020140105142A 2014-08-13 2014-08-13 Sample Chamber Cartridge for Reducing Field Curvature KR101627337B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
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US10274742B2 (en) 2017-03-29 2019-04-30 Samsung Display Co., Ltd. Display device

Citations (3)

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KR100234236B1 (en) * 1993-03-31 1999-12-15 윤종용 Optical device with variable magnification
JP3151516B2 (en) * 1996-09-30 2001-04-03 テクトロニクス・インコーポレイテッド Electrode forming method of plasma addressed liquid crystal panel and channel member
JP3160234U (en) 2010-04-06 2010-06-17 株式会社島津製作所 Sample cell and particle size distribution measuring apparatus using the same

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JPH03151516A (en) * 1989-11-07 1991-06-27 Mazda Motor Corp Exhaust device for multiple cylinder engine
JP3646494B2 (en) * 1997-11-14 2005-05-11 凸版印刷株式会社 Cell for measuring optical properties of liquid and method for producing the same
US20100008196A1 (en) 2004-12-27 2010-01-14 Koninklijke Philips Electronics, N.V. Aberration correcting apparatus
KR100844350B1 (en) 2007-01-09 2008-07-07 주식회사 디지탈바이오테크놀러지 A chip having microchannel for counting specific micro particles among floating micro particle mixture by optical means and a method for counting micro particles using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100234236B1 (en) * 1993-03-31 1999-12-15 윤종용 Optical device with variable magnification
JP3151516B2 (en) * 1996-09-30 2001-04-03 テクトロニクス・インコーポレイテッド Electrode forming method of plasma addressed liquid crystal panel and channel member
JP3160234U (en) 2010-04-06 2010-06-17 株式会社島津製作所 Sample cell and particle size distribution measuring apparatus using the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10274742B2 (en) 2017-03-29 2019-04-30 Samsung Display Co., Ltd. Display device

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