CN105154322B - Microbe inoculation device capable of automatically identifying culture dish and identifying method - Google Patents
Microbe inoculation device capable of automatically identifying culture dish and identifying method Download PDFInfo
- Publication number
- CN105154322B CN105154322B CN201510568596.XA CN201510568596A CN105154322B CN 105154322 B CN105154322 B CN 105154322B CN 201510568596 A CN201510568596 A CN 201510568596A CN 105154322 B CN105154322 B CN 105154322B
- Authority
- CN
- China
- Prior art keywords
- culture dish
- identification
- image
- identifying
- microbe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of microbe inoculation, and particularly relates to a microbe inoculation device capable of automatically identifying a culture dish and an identifying method. The microbe inoculation device capable of automatically identifying the culture dish comprises the culture dish and an inoculating instrument and is characterized in that the culture dish is of a colorless transparent disposable round plastic structure, the edge of the surface of an upper cover of the culture dish is provided with an image identifier matched with the culture dish, the inoculating instrument is provided with a photograph system, the photograph system is connected with an image identifying processing system, and the image identifying processing system is connected with a data storage system. The device and the method have the beneficial effects that whether the culture dish is a required one can be quickly determined, and a mistake is avoided; the operation is simple, the working efficiency is high, the cost is low, waste is avoided, the accuracy and stability of a test result are guaranteed, the difficult matching problem of an existing automatic microbe inoculating instrument and the culture dish is solved, and the device and the method are suitable for being widely popularized and applied.
Description
（One）Technical field
The invention belongs to microbial inoculant technical field, more particularly to it is a kind of can automatic identification culture dish microbial inoculant
Device and recognition methodss.
（Two）Background technology
Inoculation is by the process of purpose microorganism transposing to culture matrix by aseptic technique requirement.At present, microorganism
Inoculation is completing, there is many defects by manually：The different periods of personal level difference, personal habits and same person
The factor such as randomness, affect effect of inoculation, increased the difficulty of doctor's judgement；Efficiency is low, high cost, high labor intensive；Behaviour
When making personnel's operation must extreme care, be otherwise likely to pathogenic bacteria and be stained with to hand or with splashing, health has been produced
Raw harm.
Nowadays, be to solve automatic vaccination device to be occurred in that on problem above market, but equally exist in actual use scarce
Fall into：Inoculation instrument is in inoculation, it may be desirable to various different culture medium, and different purpose microorganisms needs transposing to specifically
In culture medium, the problem that instrument often occurs wrong culture medium in seeded process is inoculated with, effect of inoculation has been had a strong impact on even
Irremediable loss can be caused.Additionally, inoculation instrument and culture dish are all matching used, because being inoculated with when instrument is manufactured with regard to pin
The characteristics of to its culture dish, is equipped with corresponding system and function, if arbitrarily changing different culture dishs, will be to being inoculated with instrument
Cause to damage, can also affect the stability and accuracy of experimental result；It is used to be inoculated with connecing for instrument using disposable circular culture dish
It is the national classical way advocated to plant, and culture dish is water white transparency, and beneficial to the morphological characteristic of observation culture, analysis grows feelings
Condition.If arbitrarily changing the culture dish that automatization's classification inoculation apparatus match, the operation sequence of classification inoculation apparatus setting themselves can be affected,
Line error is easily caused, and causes the phenomenons such as result mistake.On the other hand, also can bring greatly not to clinical manipulation personnel
Just, and it is unfavorable for the work such as the equal comparation and assessment between hospital.
（Three）The content of the invention
The present invention is in order to make up the deficiencies in the prior art, there is provided a kind of simple to operate, high working efficiency, reduces cost,
Avoid wasting, ensure experimental result accurate stable can automatic identification culture dish microbe inoculating device and recognition methodss.
The present invention is achieved through the following technical solutions：
It is a kind of can automatic identification culture dish microbe inoculating device, including culture dish and inoculation instrument, it is characterized in that：It is described
Culture dish is water white disposable round plastic structure, and the edge of the upper lid surface of culture dish is provided with matching figure
Mark, inoculation instrument be provided with camera system, camera system is connected with image recognition processing system, image recognition processing system and
Data-storage system is connected.
The data-storage system is connected with computer.
It is a kind of can automatic identification culture dish recognition methodss, it is characterized in that：Comprise the following steps：
（1）, pre-build required culture dish pattern identification and take the lid corresponding database information of operation, non-required culture
Ware pattern identification database information corresponding with power operation is simultaneously stored；
（2）, culture dish taken pictures, obtain image, and the image information to obtaining be identified, if identification one
Cause, then guidance carries out next step and takes lid operation；If identification is inconsistent, system is instructed to carry out power operation.
Preferably, in step（1）In, type of culture medium, use date, culture dish accumulation are input in the data base and use number
Amount and number information.
The invention has the beneficial effects as follows：Can the automatic decision whether culture medium for needed for rapidly, prevent error；Simple to operate,
High working efficiency, reduces cost, avoid wasting, ensure that experimental result accurate stable, solving existing automatic microorganism inoculation
The difficult problem that instrument is matched with culture dish, is suitable to wide popularization and application.
（Four）Description of the drawings
The present invention is further illustrated below in conjunction with the accompanying drawings.
Structured flowchart of the accompanying drawing 1 for the present invention.
（Five）Specific embodiment
Accompanying drawing is a kind of specific embodiment of the present invention.The embodiment includes culture dish and inoculation instrument, it is characterized in that：It is described
Culture dish is water white disposable round plastic structure, and the edge of the upper lid surface of culture dish is provided with matching figure
Mark, it is easy to the growing state of later observations culture, inoculation instrument are provided with camera system, camera system and image recognition processing
System is connected, and image recognition processing system is connected with data-storage system.Data-storage system is connected with computer.
Using the present invention it is a kind of can automatic identification culture dish microbe inoculating device, in advance in data-storage system
The specificity pattern identification database information corresponding with " taking lid operation " is set up, is set up corresponding to non-specific pattern identification
The database information of " power operation " is simultaneously stored.By camera system be arranged on culture dish uncap operation before, shoot culture dish on
Lid, is obtained image, and the image information for obtaining is identified by image recognition processing system.If identification is consistent, refer to
Leading carries out next step operation " taking lid operation "；If identification is inconsistent, corresponding system " power operation " is instructed.Database information
In also include：The accumulative usage quantity of type of culture medium, use date, culture dish and corresponding numbering.By data-storage system and meter
Calculation machine connects, and can read, store, print data storehouse information.
Claims (4)
1. it is a kind of can automatic identification culture dish microbe inoculating device, including culture dish and inoculation instrument, it is characterized in that：The training
Foster ware is water white disposable round plastic structure, and the edge of the upper lid surface of culture dish is provided with matching figure mark
Know, inoculation instrument is provided with camera system, and camera system is connected with image recognition processing system, image recognition processing system and number
It is connected according to storage system.
2. it is according to claim 1 it is a kind of can automatic identification culture dish microbe inoculating device, it is characterized in that：The number
It is connected with computer according to storage system.
3. it is a kind of according to claim 1 or 2 any one devices carry out can automatic identification culture dish recognition methodss, its feature
It is：Comprise the following steps：
（1）, pre-build required culture dish pattern identification and take the lid corresponding database information of operation, non-required culture dish figure
Shape identifies database information corresponding with power operation and stores；
（2）, culture dish taken pictures, obtain image, and the image information to obtaining be identified, if identification is consistent,
Guidance carries out next step and takes lid operation；If identification is inconsistent, system is instructed to carry out power operation.
4. it is according to claim 3 it is a kind of can automatic identification culture dish recognition methodss, it is characterized in that：In step（1）In,
Type of culture medium, use date, culture dish accumulation usage quantity and number information are input in data base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510568596.XA CN105154322B (en) | 2015-09-09 | 2015-09-09 | Microbe inoculation device capable of automatically identifying culture dish and identifying method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510568596.XA CN105154322B (en) | 2015-09-09 | 2015-09-09 | Microbe inoculation device capable of automatically identifying culture dish and identifying method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105154322A CN105154322A (en) | 2015-12-16 |
| CN105154322B true CN105154322B (en) | 2017-04-19 |
Family
ID=54795376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510568596.XA Active CN105154322B (en) | 2015-09-09 | 2015-09-09 | Microbe inoculation device capable of automatically identifying culture dish and identifying method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105154322B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118950B (en) * | 2017-05-06 | 2019-10-11 | 广西梧州圣源茶业有限公司 | Automatic bacterial inoculation instrument |
| CN111024967A (en) * | 2019-12-19 | 2020-04-17 | 济南百博生物技术股份有限公司 | Sample identification device and identification method of microorganism pretreatment system |
| CN112725168A (en) * | 2021-01-08 | 2021-04-30 | 湖南隋侯珠科技有限公司 | Automatic processing system for microbial samples |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912596A (en) * | 1972-03-09 | 1975-10-14 | Int Foundation Of Microbiology | Multiple inoculating system |
| CN201704329U (en) * | 2010-06-17 | 2011-01-12 | 于大胜 | Microorganism incubator on automatic constant touch agar surface |
| CN102757891A (en) * | 2011-04-28 | 2012-10-31 | 魏志勇 | Automatic microbial inoculation instrument |
| CN202643695U (en) * | 2012-05-31 | 2013-01-02 | 徐志全 | Culture dish with label |
| CN203320010U (en) * | 2013-02-24 | 2013-12-04 | 王敬华 | Automatic rapid multi-channel bacterial inoculating instrument |
| CN104616115A (en) * | 2015-02-13 | 2015-05-13 | 上海市同济医院 | Laboratory microbial culture management system and method and bar code coding method thereof |
| CN205046117U (en) * | 2015-09-09 | 2016-02-24 | 济南百博生物技术股份有限公司 | But microorganism of automatic identification culture dish inoculation device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050277169A1 (en) * | 2004-05-14 | 2005-12-15 | Sigmund Janet M | Active microcosm: screening antimicrobial producing microorganisms |
-
2015
- 2015-09-09 CN CN201510568596.XA patent/CN105154322B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912596A (en) * | 1972-03-09 | 1975-10-14 | Int Foundation Of Microbiology | Multiple inoculating system |
| CN201704329U (en) * | 2010-06-17 | 2011-01-12 | 于大胜 | Microorganism incubator on automatic constant touch agar surface |
| CN102757891A (en) * | 2011-04-28 | 2012-10-31 | 魏志勇 | Automatic microbial inoculation instrument |
| CN202643695U (en) * | 2012-05-31 | 2013-01-02 | 徐志全 | Culture dish with label |
| CN203320010U (en) * | 2013-02-24 | 2013-12-04 | 王敬华 | Automatic rapid multi-channel bacterial inoculating instrument |
| CN104616115A (en) * | 2015-02-13 | 2015-05-13 | 上海市同济医院 | Laboratory microbial culture management system and method and bar code coding method thereof |
| CN205046117U (en) * | 2015-09-09 | 2016-02-24 | 济南百博生物技术股份有限公司 | But microorganism of automatic identification culture dish inoculation device |
Non-Patent Citations (1)
| Title |
|---|
| PREVI Isola自动接种仪的应用评价;王春玉等;《国际检验医学杂志》;20131030;第34卷(第20期);第2748-2749页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105154322A (en) | 2015-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104616115B (en) | Laboratory microculture management system and management method | |
| Crusz et al. | Bursting the bubble on bacterial biofilms: a flow cell methodology | |
| CN105154322B (en) | Microbe inoculation device capable of automatically identifying culture dish and identifying method | |
| ATE508426T1 (en) | COUNTING BIOLOGICAL AGENTS ON BIOLOGICAL GROWING PLATES | |
| CN106459859A (en) | Octagonal pillar-shaped cell culture container | |
| CN110706203A (en) | Method and system for automatically detecting key points of lateral skull tablets based on deep learning | |
| CN105158429A (en) | Method for measuring content of biodegradable organic carbon (BDOC) in water | |
| CN215082724U (en) | Device capable of reading multiple biological indicators | |
| CN116189919B (en) | Computer analysis method and system for microbial drug sensitivity and application of computer analysis method and system | |
| CN108753596A (en) | A kind of microorganism growth image detection microchamber microfluidic system | |
| CN205046117U (en) | But microorganism of automatic identification culture dish inoculation device | |
| CN201313888Y (en) | Microbe inoculation-cultivation device | |
| CN203346379U (en) | Simple bacterial biofilm generation device | |
| US20230083087A1 (en) | Microorganic detection system with worklists | |
| CN211847953U (en) | Microorganism observation test card and observation system | |
| CN208395189U (en) | A kind of incubator with image analysis function | |
| CN108285866A (en) | A kind of incubator with image analysis function | |
| CN208200979U (en) | Germ culturing device is used in a kind of experiment culture and detection | |
| CN204417516U (en) | The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope | |
| CN210329120U (en) | Biological sample freezes deposits intraductal identification means that embeds | |
| CN203284402U (en) | Dedicated disposable plastic culture dish for medicine anti-microbial experiment paper-dish method | |
| CN206814764U (en) | A kind of livestock and poultry infectious disease germ fast culture ware | |
| Young et al. | Individual susceptibility to neisserial infection? | |
| CN202063921U (en) | Full-automatic blood culture instrument | |
| CN205413121U (en) | Bioengineering is with rotatory test tube placement rack of full -automatic multilayer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 250101 workshop 303, building 5, industrialization base of small and medium sized enterprises in biological medicine Park, 1777 Dazheng Road, high tech Zone, Jinan City, Shandong Province Patentee after: JINAN BAIBO BIOTECHNOLOGY Co.,Ltd. Address before: 250101 C, 802, century wealth center, east of Chong Hua Road, Ji'nan hi tech Zone, Shandong, China Patentee before: JINAN BAIBO BIOTECHNOLOGY Co.,Ltd. |