CN107267374A - Culture apparatus is transported in a kind of clinical sample collection - Google Patents

Culture apparatus is transported in a kind of clinical sample collection Download PDF

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Publication number
CN107267374A
CN107267374A CN201710644355.8A CN201710644355A CN107267374A CN 107267374 A CN107267374 A CN 107267374A CN 201710644355 A CN201710644355 A CN 201710644355A CN 107267374 A CN107267374 A CN 107267374A
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CN
China
Prior art keywords
culture
swab
test tube
transported
pipe cap
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710644355.8A
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Chinese (zh)
Inventor
刘柳
佟曦
佟静杰
佟乐
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Changchun Jiexi Medical Science & Technology Co Ltd
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Changchun Jiexi Medical Science & Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Changchun Jiexi Medical Science & Technology Co Ltd filed Critical Changchun Jiexi Medical Science & Technology Co Ltd
Priority to CN201710644355.8A priority Critical patent/CN107267374A/en
Publication of CN107267374A publication Critical patent/CN107267374A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention belongs to medical instruments field, and in particular to culture apparatus is transported in a kind of clinical sample collection, and the device includes swab and culture tube;The swab includes pipe cap and sampling cotton swab, and the sampling cotton swab afterbody is adhesively fixed in pipe cap inner bottom surface center position;The culture tube includes test tube and cap test tube, and the test tube is built with culture medium;The collection is transported culture apparatus and is stored in disposable sterilized pack.The present invention can be used for human body natural's cavity secretion, skin exudates, surface of a wound exudate, the collection of wound tissue fluid sample, transport.Simple in construction, good leak tightness of the invention, it is to avoid cross pollution is polluted by other pollution sources, improves accuracy rate;It can also effectively avoid the apoptosis of in vitro sample bacterium simultaneously.The germ in sample can be avoided to occur leakage pollution, it is to avoid it is to environment and medical personnel, the secondary pollution of patient, it is ensured that the environmental sanitation of hospital.

Description

Culture apparatus is transported in a kind of clinical sample collection
Technical field
The invention belongs to medical instruments field, and in particular to culture apparatus is transported in a kind of clinical sample collection.
Background technology
Clinically deposit, transport in opening mostly after sample collection at present, the sample of upper machine needs to save bit by bit enough starts Row start is examined again after quantity, and the sample of Bacteria Culture is also that open be transported to behind laboratory could start inoculated and cultured.In sample In the open environment that product are deposited or transported, sample cross contamination or happened occasionally by the event that other pollution sources pollute.In addition, The sample collected can cause the germ apoptosis to be measured of fragility in vitro for a long time, and its result can cause corpse or other object for laboratory examination and chemical testing Sample result Pseudo- positive or under-referral, clinically assay puppet is positive or under-referral is formulated therapeutic scheme and will misled, and its result is very To being fatal.
The content of the invention
In order to overcome above-mentioned deficiency, culture apparatus is transported the invention provides a kind of collection of clinical sample:
Culture apparatus is transported in a kind of clinical sample collection includes swab and culture tube;The swab includes pipe cap and sampling cotton Label, the sampling cotton swab afterbody is adhesively fixed in pipe cap inner bottom surface center position;The culture tube includes test tube and cap test tube, The test tube is built with culture medium;The collection is transported culture apparatus and is stored in disposable sterilized pack.
Pipe cap on the swab is used during collecting sample as the clamping part of swab, and the pipe cap and culture Cap test tube inner and outer diameter on pipe is equal, and is threadably connected with test tube.
Pipe cap on the swab is to be axially inserted into test tube and when screwing pipe cap, sampling cotton swab is stretched into culture medium.
The culture medium prescription is:
The culture medium prescription further optimized is:
It is described to pack as paper plastic bag.
The present invention is removed pack in use first, and hand-held swab pipe cap is sampled, will culture after sampling Pipe cap test tube is opened, and is rapidly directly axially inserted into test tube the pipe cap with sampling cotton swab, is screwed and sample is may wait for after pipe cap The transport of product.In the process, the microorganism on sampling cotton swab is to be survived using the culture medium in test tube.
The present invention is separated with culture tube by swab, is to ensure the culture medium in culture tube using preceding holding nothing Bacterium state, it is to avoid integrated device is opened during pipe cap gathered using swab, invisible spectro culture in open state The contaminated phenomenon of base.
The present invention is available for human body natural's cavity secretion, skin exudates, surface of a wound exudate, wound tissue fluid sample Collection, transport.
A kind of clinical sample collection transport culture apparatus of the present invention is simple in construction, and good leak tightness is straight after sample collection is in vitro Connect and rapid be put into that culture medium is medium to be tested or culture, it is to avoid cross pollution is polluted by other pollution sources, improves accuracy rate;Together When can also effectively avoid the apoptosis of in vitro sample bacterium.The present invention can be kept away carrying out closed storage after in vitro sample collection Exempt from germ in sample and occur leakage pollution, it is to avoid it is to environment and medical personnel, the secondary pollution of patient, it is ensured that hospital Environmental sanitation.
Brief description of the drawings
Fig. 1 is the structural representation that culture apparatus is transported in a kind of clinical sample collection.
In figure:1st, culture apparatus is transported in collection;2nd, swab;3rd, culture tube;4th, pipe cap;5th, sampling cotton swab;6th, test tube;7th, try Pipe cap;8th, culture medium;9th, pack.
Embodiment
Technical scheme is described further with reference to embodiment, but is not limited thereto, it is every right Technical solution of the present invention is modified or equivalent substitution, without departing from the spirit and scope of technical solution of the present invention, all should be contained Cover in protection scope of the present invention.
Culture apparatus is transported in a kind of clinical sample collection includes swab 2 and culture tube 3;The swab 2 includes pipe cap 4 and adopted Sample cotton swab 5, the sampling afterbody of cotton swab 5 is adhesively fixed in the inner bottom surface center position of pipe cap 4;The culture tube 3 includes test tube 6 With cap test tube 7, the test tube 6 is built with culture medium 8;The collection transports culture apparatus and is stored in disposable sterilized pack In 9.
Pipe cap 4 on the swab 2 is used during collecting sample as the clamping part of swab 2, and the pipe cap 4 with The inner and outer diameter of cap test tube 7 on culture tube 3 is equal, and is threadably connected with test tube 6.
Pipe cap 4 on the swab is to be axially inserted into test tube 6 and when screwing pipe cap 4, sampling cotton swab 5 is stretched into culture medium 8.
Culture medium prescription is in apparatus of the present invention:
Culture medium preparation method is as follows:
First, partial purification water is measured, peptone is moved into and is heated in 45 DEG C ± 2 DEG C of purified water, soaking at room temperature 24h, Resulting solution is standby;
2nd, serum broth, peptone, beef extract, glucose, sucrose, magnesium sulfate, growth factor, biphosphate are measured Sodium, sodium chloride and agar are moved into step one resulting solution, boil 15min, add starch, and gained culture medium is colourless or light color Gel, without insoluble foreign matter;
3rd, culture medium obtained by step 2 is filled into test tube, every test tube adds (4 ± 0.5) mL, covers tightly cap test tube;
4th, the test tube dispensed combines with the swab with sampling cotton swab and uses paper bag hermetic package, is irradiated in 4h Sterilizing, irradiation dose 10G obtains clinical sample collection of the present invention and transports culture apparatus after sterilizing.
The present invention is removed pack in use first, and hand-held swab pipe cap is sampled, will culture after sampling Pipe cap test tube is opened, and is rapidly directly axially inserted into test tube the pipe cap with sampling cotton swab, is screwed and sample is may wait for after pipe cap The transport of product.In the process, the microorganism on sampling cotton swab is to be survived using the culture medium in test tube.
Described device can be used for throat, vagina, a wound corpse or other object for laboratory examination and chemical testing and eisseria, streptococcus pneumonia and streptococcus, white The collection of larynx bacillus, Salmonella and Shigella is transported, or excrement and adopting for a rectum corpse or other object for laboratory examination and chemical testing are examined and campylobacter jejuni, arc Pseudomonas, the collection of salmonella and shigella are transported.
Embodiment 1:
Culture apparatus is transported in a kind of clinical sample collection includes swab and culture tube;The swab includes pipe cap and sampling cotton Label, the sampling cotton swab afterbody is adhesively fixed in pipe cap inner bottom surface center position;The culture tube includes test tube and cap test tube, The test tube is built with culture medium;The collection is transported culture apparatus and is stored in disposable sterilized pack.
Pipe cap on the swab is used during collecting sample as the clamping part of swab, and the pipe cap and culture Cap test tube inner and outer diameter on pipe is equal, and is threadably connected with test tube.
Pipe cap on the swab is to be axially inserted into test tube and when screwing pipe cap, sampling cotton swab is stretched into culture medium.
Culture medium prescription is in apparatus of the present invention:
Culture medium preparation method is as follows:
First, partial purification water is measured, peptone is moved into and is heated in 45 DEG C of purified water, soaking at room temperature 24h, gained is molten Liquid is standby;
2nd, serum broth, peptone, beef extract, glucose, sucrose, magnesium sulfate, growth factor, biphosphate are measured Sodium, sodium chloride and agar are moved into step one resulting solution, boil 15min, add starch, and gained culture medium is colourless or light color Gel, without insoluble foreign matter;
3rd, culture medium obtained by step 2 is filled into test tube, every test tube adds 4mL, covers tightly cap test tube;
4th, the test tube dispensed combines with the swab with sampling cotton swab and uses paper bag hermetic package, is irradiated in 4h Sterilizing, irradiation dose 10G obtains clinical sample collection of the present invention and transports culture apparatus after sterilizing.

Claims (6)

1. culture apparatus is transported in a kind of clinical sample collection, it is characterised in that:The device includes swab (2) and culture tube (3);Institute Stating swab (2) includes pipe cap (4) and sampling cotton swab (5), and described sampling cotton swab (5) afterbody is adhesively fixed in pipe cap (4) inner bottom surface Center position;The culture tube (3) includes test tube (6) and cap test tube (7), and the test tube (6) is built with culture medium (8);Institute State collection transport culture apparatus (1) and be stored in disposable sterilized pack in (9).
2. culture apparatus is transported in a kind of clinical sample collection according to claim 1, it is characterised in that on the swab (2) Pipe cap (4) is used during collecting sample as the clamping part of swab (2), and the pipe cap (4) and the examination on culture tube (3) Pipe cap (7) inner and outer diameter is equal, and is threadably connected with test tube (6).
3. culture apparatus is transported in a kind of clinical sample collection according to claim 1, it is characterised in that on the swab (2) Pipe cap (4) is to be axially inserted into test tube (6) and when screwing pipe cap (4), sampling cotton swab (5) is stretched into culture medium (8).
4. culture apparatus is transported in a kind of clinical sample collection according to claim 1, it is characterised in that the culture medium prescription For:
5. culture apparatus is transported in a kind of clinical sample collection according to claim 1, it is characterised in that the culture medium prescription For:
6. culture apparatus is transported in a kind of clinical sample collection according to claim 1, it is characterised in that it is described pack for Paper plastic bag.
CN201710644355.8A 2017-08-01 2017-08-01 Culture apparatus is transported in a kind of clinical sample collection Pending CN107267374A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710644355.8A CN107267374A (en) 2017-08-01 2017-08-01 Culture apparatus is transported in a kind of clinical sample collection

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CN201710644355.8A CN107267374A (en) 2017-08-01 2017-08-01 Culture apparatus is transported in a kind of clinical sample collection

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CN107267374A true CN107267374A (en) 2017-10-20

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753591A (en) * 2018-05-30 2018-11-06 天津达伟医疗科技有限公司 A kind of inspection of Integral anal increases bacterium probe tube, method and purposes
CN110093250A (en) * 2019-05-14 2019-08-06 滕文友 A kind of full-automatic Culture Mycoplasma sample-adding detector and its culture sample-adding detection method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090004688A1 (en) * 2007-06-29 2009-01-01 Mciver Dawn E Sampling System and Method
CN201962276U (en) * 2010-12-27 2011-09-07 青岛高科技工业园海博生物技术有限公司 Transporting culture medium
CN102232851A (en) * 2011-05-26 2011-11-09 马均彪 Microbe sampling enrichment culture tube
CN105420341A (en) * 2016-01-04 2016-03-23 长春呈实健康实业有限公司 B hemolytic streptococcus screening reagent, preparation method thereof and B hemolytic streptococcus screening method
CN206204313U (en) * 2016-11-21 2017-05-31 北京基因树科技有限公司 Oral cavity sampling pipe

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090004688A1 (en) * 2007-06-29 2009-01-01 Mciver Dawn E Sampling System and Method
CN201962276U (en) * 2010-12-27 2011-09-07 青岛高科技工业园海博生物技术有限公司 Transporting culture medium
CN102232851A (en) * 2011-05-26 2011-11-09 马均彪 Microbe sampling enrichment culture tube
CN105420341A (en) * 2016-01-04 2016-03-23 长春呈实健康实业有限公司 B hemolytic streptococcus screening reagent, preparation method thereof and B hemolytic streptococcus screening method
CN206204313U (en) * 2016-11-21 2017-05-31 北京基因树科技有限公司 Oral cavity sampling pipe

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753591A (en) * 2018-05-30 2018-11-06 天津达伟医疗科技有限公司 A kind of inspection of Integral anal increases bacterium probe tube, method and purposes
CN108753591B (en) * 2018-05-30 2022-03-22 天津达伟医疗科技有限公司 Integrated anal detection enriched bacteria sampling tube, method and application
CN110093250A (en) * 2019-05-14 2019-08-06 滕文友 A kind of full-automatic Culture Mycoplasma sample-adding detector and its culture sample-adding detection method

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