CN105420341A - B hemolytic streptococcus screening reagent, preparation method thereof and B hemolytic streptococcus screening method - Google Patents
B hemolytic streptococcus screening reagent, preparation method thereof and B hemolytic streptococcus screening method Download PDFInfo
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- CN105420341A CN105420341A CN201610004150.9A CN201610004150A CN105420341A CN 105420341 A CN105420341 A CN 105420341A CN 201610004150 A CN201610004150 A CN 201610004150A CN 105420341 A CN105420341 A CN 105420341A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/14—Streptococcus; Staphylococcus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
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Abstract
The invention discloses a B hemolytic streptococcus screening reagent, a preparation method thereof and a B hemolytic streptococcus screening method, and belongs to the field of B hemolytic streptococcus screening. The technical problems that an existing hemolytic streptococcus screening method is complex in detection process, long in time and high in cost are solved. The B hemolytic streptococcus screening reagent is prepared by mixing serum broth, peptone, starch, a beef extract, glucose, magnesium sulfate, growth factor agar, serum and a phosphate buffer. The screening method comprises the steps of 1, collecting a sample; 2, directly moving the collected sample into a culture medium test tube of the B hemolytic streptococcus screening reagent specified in the claim 1, performing sealing, feeding the sample to an incubator as soon as possible at the temperature of 37 DEG C for culturing for 2-24 h, and observing the color of the reagent, wherein the sample is a positive sample if the color is changed and is a negative sample if the color is not changed. The B hemolytic streptococcus screening reagent is used for screening the B hemolytic streptococcus.
Description
Technical field
The invention belongs to B Hemolytic streptococcus examination field; Be specifically related to a kind of Hemolytic streptococcus screening agent and preparation method thereof and screening method.
Background technology
B race Hemolytic streptococcus is a kind of gram positive coccus, mainly based on the pathogenic bacterium of the urogenital tract and lower digestive tract that colonize in people.In recent years, because B race Hemolytic streptococcus causes the situation of genital infection increasing, particularly perinatal infection constantly presents ascendant trend.Therefore, Clinical significance of detecting carries out to B race Hemolytic streptococcus great.
According to national standard B Hemolytic streptococcus inspection process (GB4789.11-2014), checkout procedure is complicated, length consuming time (18h-24h), sample cost high (serum 25g).
Summary of the invention
The present invention will solve the technical problem that existing Hemolytic streptococcus detection method testing process is complicated, the time is long and cost is high; And provide B Hemolytic streptococcus screening agent and screening method.
B Hemolytic streptococcus screening agent of the present invention is made up of following raw materials according: serum broth: 3.0 ~ 6.0g, peptone: 25.0 ~ 35.0g, starch: 15.0 ~ 25.0g, beef extract: 2.0 ~ 5.0g, glucose: 1.0 ~ 5.0g, magnesium sulfate: 0.01 ~ 0.05g, somatomedin: 1.0 ~ 1.5g, agar: 3.0 ~ 5.0g, serum: 5 ~ 15ml and phosphate buffered saline buffer: 1000ml.
The preparation method of B Hemolytic streptococcus screening agent carries out in the steps below: room temperature 18 ~ 26 DEG C, relative humidity 45% ~ 65%, under clean rank 100,000 grades of conditions, serum broth, peptone, starch, beef extract, glucose, magnesium sulfate, somatomedin, agar, serum and phosphate buffered saline buffer are mixed, is then stirred to evenly; Namely medical sterilization ultrasonic coupling agent is obtained.
Described serum broth is suis meat soup.
Described glucose is dextrose.
The screening method of B Hemolytic streptococcus carries out in the steps below:
Step one, collecting sample: gather vaginal secretions or rectal secretions by sterile swab;
Sample after step 2, collection directly moves in the substratum test tube that B Hemolytic streptococcus screening agent described in claim 1 is housed, sealing, delivers to thermostat container 37 DEG C as early as possible and cultivates 2h ~ 24h, observe the color of reagent, variable color is positive sample, is negative sample without colour-change; Namely the examination of B Hemolytic streptococcus is completed.
The screening method of B Hemolytic streptococcus also can carry out in the steps below:
Step one, collecting sample: gather vaginal secretions and rectal secretions respectively by two sterile swab;
Sample after step 2, collection directly moves into respectively and is equipped with in the substratum test tube of B Hemolytic streptococcus screening agent described in claim 1, sealing, delivers to thermostat container 37 DEG C as early as possible and cultivates 2h ~ 24h, observe the color of reagent, variable color is positive sample, is negative sample without colour-change; Namely the examination of B Hemolytic streptococcus is completed.
B Hemolytic streptococcus screening agent of the present invention is colourless or white to micro-yellow gel, uniform color, tasteless, odorless, inclusion-free; Preserve at shady and cool dry place after airtight; Validity period is 2 years
The present invention is in the short period of time by B Hemolytic streptococcus screening agent colour-change interpretation assay, and accuracy rate is high.It is negligible that present device drops into amount; Reduce by 1/3 consumables cost and whole manual labor's time, even directly can transfer to and treat district, section office's operation; There is the function of duplicate acknowledgment detected result.
Embodiment
In embodiment one, present embodiment, B Hemolytic streptococcus screening agent is made up of following raw materials according: serum broth (suis meat soup): 5.0g, peptone: 30.0g, starch: 20.0g, beef extract: 4.0g, dextrose: 2.0g, magnesium sulfate: 0.02g, somatomedin: 1.1g, agar: 4.5g, serum: 10ml and phosphate buffered saline buffer: 1000ml;
Its preparation method carries out in the steps below:
The screening method of B Hemolytic streptococcus carries out in the steps below:
Step one, collecting sample: gather vaginal secretions or rectal secretions by a sterile swab;
Sample after step 2, collection directly moves in the substratum test tube that B Hemolytic streptococcus screening agent described in claim 1 is housed, sealing, delivers to thermostat container 37 DEG C as early as possible and cultivates 2h, observe the color of reagent, variable color is positive sample, is negative sample without colour-change; Namely the examination of B Hemolytic streptococcus is completed.
The B Hemolytic streptococcus screening agent of present embodiment is colorless gel, uniform color, tasteless, odorless, inclusion-free; Preserve at shady and cool dry place after airtight; Validity period is 2 years.
The present invention is in the short period of time by B Hemolytic streptococcus screening agent colour-change interpretation assay, and accuracy rate is high, rate of accuracy reached more than 99.99%.
Embodiment two: in present embodiment, the screening method of B Hemolytic streptococcus carries out in the steps below:
Step one, collecting sample: gather vaginal secretions and rectal secretions respectively by two sterile swab;
Sample after step 2, collection directly moves into respectively and is equipped with in the substratum test tube of B Hemolytic streptococcus screening agent described in j embodiment one, sealing, delivers to thermostat container 37 DEG C as early as possible and cultivates 2h, observe the color of reagent, variable color is positive sample, is negative sample without colour-change; Namely the examination of B Hemolytic streptococcus is completed.
The B Hemolytic streptococcus screening agent of present embodiment is colorless gel, uniform color, tasteless, odorless, inclusion-free; Preserve at shady and cool dry place after airtight; Validity period is 2 years.
The present invention is in the short period of time by B Hemolytic streptococcus screening agent colour-change interpretation assay, and accuracy rate is high, rate of accuracy reached more than 99.99%.
Claims (7)
1.B Hemolytic streptococcus screening agent, is characterized in that what B Hemolytic streptococcus screening agent was made up of following raw materials according:
Serum broth: 3.0 ~ 6.0g, peptone: 25.0 ~ 35.0g, starch: 15.0 ~ 25.0g, beef extract: 2.0 ~ 5.0g, glucose: 1.0 ~ 5.0g, magnesium sulfate: 0.01 ~ 0.05g, somatomedin: 1.0 ~ 1.5g, agar: 3.0 ~ 5.0g, serum: 5 ~ 15ml and phosphate buffered saline buffer: 1000ml.
2. B Hemolytic streptococcus screening agent according to claim 1, is characterized in that what B Hemolytic streptococcus screening agent was made up of following raw materials according:
Serum broth: 5.0g, peptone: 30.0g, starch: 20.0g, beef extract: 4.0g, glucose: 2.0g, magnesium sulfate: 0.02g, somatomedin: 1.1g, agar: 4.5g, serum: 10ml and phosphate buffered saline buffer: 1000ml.
3. B Hemolytic streptococcus screening agent according to claim 1, is characterized in that described serum broth is suis meat soup.
4. B Hemolytic streptococcus screening agent according to claim 1, is characterized in that described glucose is dextrose.
5. the preparation method of B Hemolytic streptococcus screening agent as claimed in claim 1, it is characterized in that the preparation method of B Hemolytic streptococcus screening agent carries out in the steps below: room temperature 18 ~ 26 DEG C, relative humidity 45% ~ 65%, under clean rank 100,000 grades of conditions, serum broth, peptone, starch, beef extract, glucose, magnesium sulfate, somatomedin, agar, serum and phosphate buffered saline buffer are mixed, is then stirred to evenly; Namely B Hemolytic streptococcus screening agent is obtained.
The screening method of 6.B Hemolytic streptococcus, is characterized in that the screening method of B Hemolytic streptococcus carries out in the steps below:
Step one, collecting sample: gather vaginal secretions or rectal secretions by sterile swab;
Sample after step 2, collection directly moves in the substratum test tube that B Hemolytic streptococcus screening agent described in claim 1 is housed, sealing, delivers to thermostat container 37 DEG C as early as possible and cultivates 2h ~ 24h, observe the color of reagent, variable color is positive sample, is negative sample without colour-change; Namely the examination of B Hemolytic streptococcus is completed.
The screening method of 7.B Hemolytic streptococcus screening agent, is characterized in that the screening method of B Hemolytic streptococcus carries out in the steps below:
Step one, collecting sample: gather vaginal secretions and rectal secretions respectively by two sterile swab;
Sample after step 2, collection directly moves into respectively and is equipped with in the substratum test tube of B Hemolytic streptococcus screening agent described in claim 1, sealing, delivers to thermostat container 37 DEG C as early as possible and cultivates 2h ~ 24h, observe the color of reagent, variable color is positive sample, is negative sample without colour-change; Namely the examination of B Hemolytic streptococcus is completed.
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| CN201610004150.9A CN105420341B (en) | 2016-01-04 | 2016-01-04 | B hemolytic streptococcus screening agent and preparation method thereof, the screening method of B hemolytic streptococcus |
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| CN201610004150.9A CN105420341B (en) | 2016-01-04 | 2016-01-04 | B hemolytic streptococcus screening agent and preparation method thereof, the screening method of B hemolytic streptococcus |
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| CN105420341A true CN105420341A (en) | 2016-03-23 |
| CN105420341B CN105420341B (en) | 2019-02-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434843A (en) * | 2016-10-27 | 2017-02-22 | 江南大学 | Culture medium for selectively separating and culturing group B streptococci and preparation method thereof |
| CN107267374A (en) * | 2017-08-01 | 2017-10-20 | 长春杰希医药科技有限公司 | Culture apparatus is transported in a kind of clinical sample collection |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388527A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Streptococcus agalactiae selective chromogenic culture medium and detection test paper thereof |
-
2016
- 2016-01-04 CN CN201610004150.9A patent/CN105420341B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388527A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Streptococcus agalactiae selective chromogenic culture medium and detection test paper thereof |
Non-Patent Citations (2)
| Title |
|---|
| MANUEL DE LA ROSA等: "Granada Medium for Detection and Identification of Group B Streptococci", 《JOURNAL OF CLINICAL MICROBIOLOGY》 * |
| MANUEL DE LA ROSA等: "New Granada Medium for Detection and Identification of Group B Streptococci", 《JOURNAL OF CLINICAL MICROBIOLOGY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434843A (en) * | 2016-10-27 | 2017-02-22 | 江南大学 | Culture medium for selectively separating and culturing group B streptococci and preparation method thereof |
| CN107267374A (en) * | 2017-08-01 | 2017-10-20 | 长春杰希医药科技有限公司 | Culture apparatus is transported in a kind of clinical sample collection |
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| CN105420341B (en) | 2019-02-19 |
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Denomination of invention: B hemolytic streptococcus screening reagent, preparation method thereof and B hemolytic streptococcus screening method Effective date of registration: 20190918 Granted publication date: 20190219 Pledgee: Jilin Zhongxin science and technology credit Company limited by guarantee Pledgor: CHANGCHUN CHENGSHI HEALTH INDUSTRY Co.,Ltd. Registration number: Y2019220000014 |
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Change date: 20230620 Registration number: Y2019220000014 Pledgee after: Jilin Zhongxin Technology Credit Financing Guarantee Co.,Ltd. Pledgee before: Jilin Zhongxin science and technology credit Company limited by guarantee |
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Date of cancellation: 20230707 Granted publication date: 20190219 Pledgee: Jilin Zhongxin Technology Credit Financing Guarantee Co.,Ltd. Pledgor: CHANGCHUN CHENGSHI HEALTH INDUSTRY Co.,Ltd. Registration number: Y2019220000014 |
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