CN107090401A - A kind of drying disposable sterilized microbial culture dish - Google Patents
A kind of drying disposable sterilized microbial culture dish Download PDFInfo
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- CN107090401A CN107090401A CN201710432746.3A CN201710432746A CN107090401A CN 107090401 A CN107090401 A CN 107090401A CN 201710432746 A CN201710432746 A CN 201710432746A CN 107090401 A CN107090401 A CN 107090401A
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- culture
- drain hole
- culture dish
- paper
- liquid drain
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Chemical & Material Sciences (AREA)
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- Biomedical Technology (AREA)
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- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to culture dish technical field, more particularly to a kind of drying disposable sterilized microbial culture dish, culture chamber is formed in the culture box, described culture chamber one end covers to form liquid drain hole, its other end covers to form lower liquid drain hole, the upper liquid drain hole runs through lower liquid drain hole through the Bacteria Culture paper in culture chamber inner section and forms two-way channel for permeating liquid, the present invention, which solves prior art, to be existed and must be operated on aseptic operating platform, specialty to testing staff requires high, equipment requirement is high and must be the problem of the inspection center operations in laboratory, with reducing operation difficulty, preserve and use time is long, the advantageous effects being directly loaded.
Description
Technical field
The invention belongs to culture dish technical field, more particularly to a kind of drying disposable sterilized microbial culture dish.
Background technology
At present, quote water enter blood before will be Jing Guo intestinal barrier effect, single pair of for dialysis patient, water and
Contacting blood realizes that within certain limits, pellicle is not limited for the material by it simply by a kind of pellicle.
With being orally ingested water ratio, hemodialysis patients are among substantial amounts of dialyzate.If containing harmful substance in dialyzate, very
Easily by dialysis membrane enter patient blood in, even if the harmful substance of low concentration, long term accumulation also result in it is chronic in
Poison.The inorganic matter or organic substance of water are dissolved in, and bacterial product is easily entered in patient's body by dialysis membrane, causes nosotoxicosis
Shape.So the bacterioscopy of water for dialysis is extremely important, conventional aseptic microculture is in the handle culture on aseptic operating platform
Base is heated, after dissolving, and is injected with needle tubing in culture dish, cooling.Open and covered on culture dish, need to check that liquid injects culture medium
On, then cover lid.It is put into constant incubator, 7 days, takes out, reads bacterial number, prior art is present must be sterile
Operated on operating desk, the specialty to testing staff requires high, equipment requirement height and must be in the inspection center operations in laboratory
Problem.
The content of the invention
The present invention provides a kind of disposable sterilized microbial culture dish, to solve to propose existing skill in above-mentioned background technology
Art, which exists, to be operated on aseptic operating platform, and the specialty to testing staff requires high, equipment requirement height and must be in laboratory
Inspection center operations the problem of.
Technical problem solved by the invention is realized using following technical scheme：A kind of drying disposable sterilized microorganism
Culture chamber is formed in culture dish, including culture box, the culture box, described culture chamber one end covers to form liquid drain hole, and its is another
One end covers to form lower liquid drain hole, the upper liquid drain hole through the Bacteria Culture paper in culture chamber inner section run through lower liquid drain hole and
Form two-way channel for permeating liquid；
Method for cultivation of bacteria：The Bacteria Culture paper in dried culture chamber is soaked through TGEA when the liquid injection for needing to detect
After upper, upper liquid drain hole and lower liquid drain hole are covered into seal cap respectively.
Further, the Micro-Organism Culture Dish after sealing is put into incubator culture at most 7 days, the bacterium training after Bacteria Culture
Foster ware determines the bacterial population on Bacteria Culture paper.
Further, the bacterial population determined on filter membrane includes the bacterial flora bacterium colony for directly counting the growth on filter membrane, meter
Calculate the total bacterial flora number contained in unit water sample.
Further, the Bacteria Culture paper is that dry Bacteria Culture paper is soaked through TGEA.
Further, the inlet opening and fluid hole distinguish sealed cap sealing.
Further, covered in the culture box mouth connection.
Further, the feed pathway injection fluid to be measured.
Further, the feed pathway injection fluid to be measured is connected to including the input of inlet opening one end inserts its syringe needle,
The output of its other end is connected to culture chamber.
Further, the microorganism of fluid to be measured is cultivated in the culture chamber on Bacteria Culture paper.
Further, the Bacteria Culture paper is the butt TGEA of culture of directly adding water.
Advantageous effects：
1st, this patent in the culture box using culture chamber is formed, and described culture chamber one end covers to form liquid drain hole, and its is another
Lower liquid drain hole is formed on end cap, the upper liquid drain hole runs through lower liquid drain hole and shape through the Bacteria Culture paper in culture chamber inner section
It is in pairs to channel for permeating liquid, the Bacteria Culture paper is that dry Bacteria Culture paper, the inlet opening and fluid hole are soaked through TGEA
Covered in sealed cap sealing respectively, the culture box mouth connection, the feed pathway injects fluid to be measured, the feed pathway note
Enter fluid to be measured including inlet opening one end input be connected to insertion its syringe needle, its other end output be connected to culture chamber, it is described
The microorganism of fluid to be measured is cultivated in culture chamber on Bacteria Culture paper, the culture dish inserted in incubator, due to can be general
Operated in logical working environment, the specialty to testing staff requires low, common employee can directly operate by training, therefore, general
Logical inspector can be without on super-clean bench again, direct performance tests water quality is easy to operate.Reduce the operative configuration examined.
2nd, this patent is when dried training is soaked in the liquid injection for needing to detect through TGEA using method for cultivation of bacteria
After supporting on the Bacteria Culture paper of intracavitary, upper liquid drain hole and lower liquid drain hole are covered into seal cap respectively, by the Bacteria Culture after sealing
Ware is put into incubator culture at most 7 days, and the Micro-Organism Culture Dish after Bacteria Culture determines the bacterial population on Bacteria Culture paper, described
Determine that bacterial population on filter membrane includes the bacterial flora bacterium colony for directly counting the growth on filter membrane, calculate contain in water sample total thin
Flora number, because traditional Bacteria Culture mode is that culture medium heated on aseptic operating platform, after dissolving, is injected with needle tubing and trained
Support in ware, cooling.Open and covered on culture dish, need to check on liquid injection culture medium, then cover lid.It is put into incubated
In case, 7 days, take out, read bacterial number, cultural method of the invention need not be to medium treatments such as culture medium heating, directly
Add water culture, greatly reduces operation difficulty.
3rd, this patent is that dry Bacteria Culture paper is soaked through TGEA using the Bacteria Culture paper, due to selecting butt
TGEA, directly added water culture, and general T GEA sterilizings are used on the day of being only capable of, and basal culture medium can preserve 1 year and use time is long.
4th, the collocation method of this patent culture medium is very simple, is configured without professional.
5th, this patent simplifies membrane filter technique, can be used directly, because membrane filter technique must work on super-clean bench, then in mistake
Filter membrane is removed on filter, the possibility for having microbiological contamination, and the present invention directly sample-adding avoids filter membrane exposure in atmosphere, reducing pollution can
Can property.
6th, this patent simplifies medium culture mode, can directly be loaded, then cultivate.
Brief description of the drawings
Fig. 1 is a kind of structural representation of drying disposable sterilized microbial culture dish of the present invention；
Fig. 2 is a kind of flow chart of the method for cultivation of bacteria of drying disposable sterilized microbial culture dish of the present invention.
Embodiment
The present invention is described further below in conjunction with accompanying drawing：
In figure：
1- is cultivated to be covered on liquid drain hole under box, 2- culture chambers, the upper liquid drain holes of 3-, 4-, 5- Bacteria Culture paper, 6- seal caps, 7-, 8-
Feed pathway, 9- incubators（It is not shown）；
S1- method for cultivation of bacteria；
S101- needs the liquid injection detected to be soaked through TGEA on the Bacteria Culture paper in dried culture chamber；
Upper liquid drain hole and lower liquid drain hole are covered seal cap by S102- respectively；
Micro-Organism Culture Dish after sealing is put into incubator culture at most 7 days, the Micro-Organism Culture Dish after Bacteria Culture by S103-
Determine the bacterial population on Bacteria Culture paper.
Embodiment：
The present embodiment：As shown in figure 1, a kind of drying disposable sterilized microbial culture dish, including culture box 1, the culture box 1
Culture chamber 2 is inside axially formed, described one end of culture chamber 2 covers to form liquid drain hole 3, and its other end covers to form lower liquid drain hole 4,
The upper liquid drain hole 3 runs through lower liquid drain hole 4 through the Bacteria Culture paper 5 in the inner section of culture chamber 2 and forms two-way channel for permeating liquid.
Because using culture chamber is formed in the culture box, described culture chamber one end covers to form liquid drain hole, and its is another
Lower liquid drain hole is formed on end cap, the upper liquid drain hole runs through lower liquid drain hole and shape through the Bacteria Culture paper in culture chamber inner section
It is in pairs to channel for permeating liquid, the Bacteria Culture paper is that dry Bacteria Culture paper, the inlet opening and fluid hole are soaked through TGEA
Covered in sealed cap sealing respectively, the culture box mouth connection, the feed pathway injects fluid to be measured, the feed pathway note
Enter fluid to be measured including inlet opening one end input be connected to insertion its syringe needle, its other end output be connected to culture chamber, it is described
The microorganism of fluid to be measured is cultivated in culture chamber on Bacteria Culture paper, the culture dish inserted in incubator, due to can be general
Operated in logical working environment, the specialty to testing staff requires low, common employee can directly operate by training, therefore, general
Logical inspector can be without on super-clean bench again, direct performance tests water quality is easy to operate.Reduce the operative configuration examined.
As shown in Fig. 2 method for cultivation of bacteria S1：When dried culture is soaked in the liquid injection for needing to detect through TGEA
On the Bacteria Culture paper of intracavitary after S101, upper liquid drain hole and lower liquid drain hole are covered into seal cap S102 respectively.
Micro-Organism Culture Dish after sealing is put into incubator culture at most 7 days, the Micro-Organism Culture Dish after Bacteria Culture is true
Determine the bacterial population S103 on Bacteria Culture paper.
Bacterial population on the determination filter membrane includes the bacterial flora bacterium colony for directly counting the growth on filter membrane, calculates unit
The total bacterial flora number contained in water sample.
Due to being when dried culture chamber is soaked in the liquid injection for needing to detect through TGEA using method for cultivation of bacteria
After on interior Bacteria Culture paper, upper liquid drain hole and lower liquid drain hole are covered into seal cap respectively, the Micro-Organism Culture Dish after sealing is put
Enter incubator culture at most 7 days, the Micro-Organism Culture Dish after Bacteria Culture determines the bacterial population on Bacteria Culture paper, the determination
Bacterial population on filter membrane includes the bacterial flora bacterium colony for directly counting the growth on filter membrane, calculates the total bacterial flora contained in water sample
Number, because traditional Bacteria Culture mode is that culture medium heated on aseptic operating platform, after dissolving, culture dish is injected with needle tubing
In, cooling.Open and covered on culture dish, need to check on liquid injection culture medium, then cover lid.It is put into constant incubator
It is interior, 7 days, take out, read bacterial number, cultural method of the invention need not directly add to medium treatments such as culture medium heating
Aquaponic, greatly reduces operation difficulty.
The Bacteria Culture paper 5 is that dry Bacteria Culture paper 5 is soaked through TGEA.
Due to simplifying membrane filter technique, it can be used directly, because membrane filter technique must work on super-clean bench, then in filter
On remove filter membrane, the possibility for having microbiological contamination, and the present invention directly sample-adding avoid filter membrane exposure in atmosphere, reduce pollution may
Property.
Due to simplifying medium culture mode, it can directly be loaded, then cultivate.
The inlet opening and fluid hole are distinguished sealed cap 6 and sealed.
The upper lid 7 of described 1 mouthful of connection of culture box.
The feed pathway 8 injects fluid to be measured.
The injection fluid to be measured of feed pathway 8 is connected to including the input of inlet opening one end inserts its syringe needle, and its is another
End output is connected to culture chamber 2.
Because the collocation method of culture medium is very simple, configured without professional.
The microorganism of fluid to be measured is cultivated in the culture chamber 2 on Bacteria Culture paper 5.
The Bacteria Culture paper is the butt TGEA of culture of directly adding water.
Due to being that dry Bacteria Culture paper is soaked through TGEA using the Bacteria Culture paper, due to selecting butt TGEA,
Directly added water culture, and general T GEA sterilizings are used on the day of being only capable of, and basal culture medium can preserve 1 year and use time is long.
Operation principle：
This patent in the culture box by forming culture chamber, and described culture chamber one end covers to form liquid drain hole, its other end
Cover to form lower liquid drain hole, the upper liquid drain hole runs through lower liquid drain hole and formation through the Bacteria Culture paper in culture chamber inner section
Two-way channel for permeating liquid, the Bacteria Culture paper is that dry Bacteria Culture paper is soaked through TGEA, the inlet opening and fluid hole point
Covered in not sealed cap sealing, the culture box mouth connection, the feed pathway injects fluid to be measured, the feed pathway injection
Fluid to be measured is connected to including the input of inlet opening one end and inserts its syringe needle, and the output of its other end is connected to culture chamber, the training
The microorganism that fluid to be measured is cultivated on intracavitary Bacteria Culture paper is supported, the culture dish is inserted in incubator, due to can be common
Operated in working environment, the specialty to testing staff requires low, common employee can directly operate by training, the present invention is solved
Prior art, which exists, operate on aseptic operating platform, and the specialty to testing staff requires that height, equipment requirement are high and necessary
The problem of the inspection center operations in laboratory, with reducing operation difficulty, preservation and use time length, having of being directly loaded
Beneficial technique effect.
Using technical scheme, or those skilled in the art is under the inspiration of technical solution of the present invention, design
Go out similar technical scheme, and reach above-mentioned technique effect, be to fall into protection scope of the present invention.
Claims (10)
1. a kind of drying disposable sterilized microbial culture dish, it is characterised in that including culture box, training is formed in the culture box
Chamber is supported, described culture chamber one end covers to form liquid drain hole, and its other end covers to form lower liquid drain hole, the upper liquid drain hole is through training
The Bacteria Culture paper on intracavitary section is supported to run through lower liquid drain hole and form two-way channel for permeating liquid；
Method for cultivation of bacteria：The Bacteria Culture paper in dried culture chamber is soaked through TGEA when the liquid injection for needing to detect
After upper, upper liquid drain hole and lower liquid drain hole are covered into seal cap respectively.
2. the drying disposable sterilized microbial culture dish of one kind according to claim 1, it is characterised in that after sealing
Micro-Organism Culture Dish is put into incubator culture at most 7 days, and the Micro-Organism Culture Dish after Bacteria Culture determines thin on Bacteria Culture paper
Bacterium number.
3. the drying disposable sterilized microbial culture dish of one kind according to claim 2, it is characterised in that the determination filter
Bacterial population on film includes the bacterial flora bacterium colony for directly counting the growth on filter membrane, calculates the total bacterium contained in unit water sample
Group's number.
4. the drying disposable sterilized microbial culture dish of one kind according to claim 1, it is characterised in that the bacterium training
Foster paper is that dry Bacteria Culture paper is soaked through TGEA.
5. the drying disposable sterilized microbial culture dish of one kind according to claim 1, it is characterised in that the inlet opening
Distinguish sealed cap sealing with fluid hole.
6. the drying disposable sterilized microbial culture dish of one kind according to claim 1, it is characterised in that the culture box
Covered in mouth connection.
7. the drying disposable sterilized microbial culture dish of one kind according to claim 1, it is characterised in that the feed liquor leads to
Road injects fluid to be measured.
8. the drying disposable sterilized microbial culture dish of one kind according to claim 7, it is characterised in that the feed liquor leads to
Road injection fluid to be measured is connected to including the input of inlet opening one end and inserts its syringe needle, and the output of its other end is connected to culture chamber.
9. the drying disposable sterilized microbial culture dish of one kind according to claim 8, it is characterised in that the culture chamber
The microorganism of fluid to be measured is cultivated on interior Bacteria Culture paper.
10. the drying disposable sterilized microbial culture dish of one kind according to claim 1, it is characterised in that the bacterium
Cultivate butt TGEA of the paper for the culture that directly adds water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710432746.3A CN107090401A (en) | 2017-06-09 | 2017-06-09 | A kind of drying disposable sterilized microbial culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710432746.3A CN107090401A (en) | 2017-06-09 | 2017-06-09 | A kind of drying disposable sterilized microbial culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107090401A true CN107090401A (en) | 2017-08-25 |
Family
ID=59639302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710432746.3A Pending CN107090401A (en) | 2017-06-09 | 2017-06-09 | A kind of drying disposable sterilized microbial culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107090401A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504719A (en) * | 2018-04-02 | 2018-09-07 | 天津施特雷生物科技股份有限公司 | A kind of novel bacterial detection method |
| CN108587881A (en) * | 2018-04-16 | 2018-09-28 | 天津施特雷生物科技股份有限公司 | A kind of membrane filtration detection culture apparatus and method |
| WO2019071235A1 (en) * | 2017-10-05 | 2019-04-11 | Soft Cell Biological Research, Llc | Microbiological culture plate |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85106361A (en) * | 1984-10-09 | 1986-04-10 | 小林制药株式会社 | The method of training Ti Shi culture dish of culturing bacterium and inspection drug susceptibility |
| CN86100926A (en) * | 1986-02-06 | 1987-08-26 | 宛吉斌 | Serial carrier type bacterial culture medium and preparation thereof |
| WO2011096659A2 (en) * | 2010-02-05 | 2011-08-11 | Jeon Min-Yong | Microorganism or cell culture container |
| CN106635766A (en) * | 2016-11-23 | 2017-05-10 | 天津施特雷生物科技股份有限公司 | Disposable membrane sterile microbiological culture dish |
-
2017
- 2017-06-09 CN CN201710432746.3A patent/CN107090401A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85106361A (en) * | 1984-10-09 | 1986-04-10 | 小林制药株式会社 | The method of training Ti Shi culture dish of culturing bacterium and inspection drug susceptibility |
| US4775628A (en) * | 1984-10-09 | 1988-10-04 | Kobayashi Pharmaceutical Co., Ltd. | Petri dish for cultivating bacteria and method of inspecting drug susceptibility |
| CN86100926A (en) * | 1986-02-06 | 1987-08-26 | 宛吉斌 | Serial carrier type bacterial culture medium and preparation thereof |
| WO2011096659A2 (en) * | 2010-02-05 | 2011-08-11 | Jeon Min-Yong | Microorganism or cell culture container |
| CN106635766A (en) * | 2016-11-23 | 2017-05-10 | 天津施特雷生物科技股份有限公司 | Disposable membrane sterile microbiological culture dish |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019071235A1 (en) * | 2017-10-05 | 2019-04-11 | Soft Cell Biological Research, Llc | Microbiological culture plate |
| CN108504719A (en) * | 2018-04-02 | 2018-09-07 | 天津施特雷生物科技股份有限公司 | A kind of novel bacterial detection method |
| CN108587881A (en) * | 2018-04-16 | 2018-09-28 | 天津施特雷生物科技股份有限公司 | A kind of membrane filtration detection culture apparatus and method |
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Application publication date: 20170825 |