CN207877751U - A kind of novel quantitative counting identification culture dish for detecting escherichia coli - Google Patents

Abstract

The utility model belongs to bacterium colony quantitative counting identification technology field, more particularly to a kind of novel quantitative counting identification culture medium and culture dish for detecting escherichia coli, the culture medium is referred to as GCM108, the culture medium belongs to kit scope, the culture medium is by peptone, bovine bile, lactose, the compositions such as bromocresol purple, the utility model, which solves prior art presence, can only judge to whether there is escherichia coli in test sample, and the problem of being unable to accurate counting, with the large intestine clump count that can quantitatively detect in test sample, it is easy to the ne ar differentiated, facilitate the advantages such as counting, the good advantageous effects of application prospect.

Description

A kind of novel quantitative counting identification culture dish for detecting escherichia coli

Technical field

The utility model belongs to bacterium colony quantitative counting identification technology field, more particularly to a kind of novel for detecting large intestine angstrom The quantitative counting identification culture medium and culture dish of uncommon bacterium.

Background technology

Currently, the detection of escherichia coli is the legal detection project of the inspections such as food, drug, water quality, in national standard and medicine There are stringent test condition and method of inspection requirement in allusion quotation,《Chinese Pharmacopoeia》Version in 2015（The micro- life of 1106 non-sterile products Object limit test：Control bacteria examination method）Provide that the culture medium that uses is Mai Kangkai fluid nutrient mediums or maconkey agar culture medium, 《Chinese Pharmacopoeia》Detection method as defined in versions in 2015：Prepared by test liquid and Zengjing Granule takes test sample, according to " non-sterile product is micro- Biological limit test：Microorganism count method（General rule 1105）" it is made 1：10 test liquids take the confession for being equivalent to 1g or 1ml test samples Test solution is seeded to appropriate volume（It is determined through method employment and suitability test (E ＆ ST)）Pancreas junket soya peptone fluid nutrient medium in, mixing, 30~35 DEG C culture 18~24 hours, selects and is separately cultured above-mentioned culture 1ml is taken to be seeded in 100ml Mai Kangkai fluid nutrient mediums, 42~44 DEG C are cultivated 24~48 hours.Take the streak inoculation of Mai Kangkai liquid cultures on maconkey agar culture medium flat plate, 30 ~35 DEG C are cultivated 18~72 hours, if as a result judging to have bacterium colony growth on maconkey agar culture medium flat plate, should be detached, is pure Change and suitable qualification test, verify whether as escherichia coli；If there is no bacterium colony growth on maconkey agar culture medium flat plate, Though or it is feminine gender to have bacterium colony growth but qualification result, sentences test sample and escherichia coli is not detected.Prior art presence can only judge The problem of whether there is escherichia coli in test sample, and being unable to accurate counting.

Invention content

The utility model provides a kind of novel quantitative counting identification method for detecting escherichia coli, above-mentioned to solve Prior art presence is proposed in background technology can only judge to whether there is escherichia coli in test sample, and be unable to accurate counting The problem of.

The technical issues of the utility model is solved is realized using following technical scheme：It is a kind of novel for detecting large intestine The quantitative counting of angstrom uncommon bacterium identifies culture medium, which is characterized in that the culture medium is culture medium GCM108, and the culture medium belongs to Kit scope, the culture medium are made of peptone, bovine bile, lactose, bromocresol purple etc..

Meanwhile the utility model also provides a kind of novel quantitative counting identification culture dish for detecting escherichia coli, The culture box mouth end connection culture ware lid, the culture dish body is interior to be formed equipped with by peptone, bovine bile, lactose, bromine cresols The culture chamber of the culture medium of the compositions such as purple, the culture medium are connected through filterings such as filter paper, filter membranes.

Further, the feed pathway of the culture dish injects escherichia coli.

Further, feed pathway injection escherichia coli includes that the input of inlet opening one end is connected to and is inserted into its needle Head, other end output are connected to culture chamber.

Further, in the culture chamber escherichia coli is cultivated on culture medium.

Advantageous effects：

1, this patent has specificity using the culture medium GCM108 to escherichia coli.Since the culture medium has very Strong selectivity, just for the detection of escherichia coli, other bacterium colonies are not easy to grow, therefore have good identification effect, and And escherichia coli can grow the ne ar for being easy to differentiate on culture medium, facilitate the advantages such as counting.

2, using culture box ware mouth end connection culture ware lid, the culture dish body is interior to be formed equipped with by albumen this patent The culture chamber of the culture medium of the compositions such as peptone, bovine bile, lactose, bromocresol purple cultivates fluid to be measured on the GCM108 culture mediums Microorganism, in culture dish merging incubator, due to needing the liquid detected with sterile needle tubing sucking, passed through with syringe needle Inlet opening injects liquid into culture dish, extracts syringe needle, then shakes culture dish, liquid is made to be evenly distributed on culture medium, Then culture dish is put into constant incubator, is taken out after cultivating 24-48h, bacterial number is read, due to can commonly work Operation in environment, requires the profession of testing staff low, and common employee can directly operate by training, therefore, common to examine Member may not need to be operated in the environment of particular requirement, can directly be tested to water quality, simple to operate.It reduces Enterprise examines the configuration of equipment and reduces that cost 3, that this patent is suitable for detections application, the application prospect such as medicine, food, water quality is good It is good, there is significant social benefit, economic benefit.

4, this patent this product has the advantages of detection is accurate, quick and high sensitivity.

Description of the drawings

Fig. 1 is that a kind of structure of the novel quantitative counting identification culture dish for detecting escherichia coli of the utility model is shown It is intended to.

Specific implementation mode

The utility model is described further below in conjunction with attached drawing：

In figure：

Cap in 1- sealings, 2- cultivate ware lid, the drying culture mediums of 3-, 4- culture chambers, the lower cap of 5- sealings, 6- inlet openings, 7- into Liquid channel, 8- culture dish reticulate patterns bottom, 9- filter membranes

Embodiment：

The present embodiment：As shown in Figure 1, the culture medium is by peptone, bovine bile, lactose, bromocresol purple, filter membrane, filter The compositions such as paper, culture dish.

Culture dish mouth end connection culture ware lid 2, the culture dish body is interior to be formed equipped with by peptone, bovine bile, breast The culture medium 3 of the compositions such as sugar, bromocresol purple, culture chamber 4, the culture medium 3 are connected through filterings such as filter paper, filter membranes.

The feed pathway 7 injects escherichia coli.

It includes that the input of 6 one end of inlet opening is connected to and is inserted into its syringe needle that the feed pathway 7, which injects escherichia coli, another One end output is connected to culture chamber 4.

In the culture chamber 4 escherichia coli is cultivated on culture medium 3.

Operation principle：

This patent is by peptone, bovine bile, lactose, bromocresol purple, filter membrane, filter paper, culture dish by the culture medium Deng composition, the culture medium has specificity to escherichia coli.Since the culture medium has very strong selectivity, just for big The detection of the uncommon bacterium of intestines angstrom, other bacterium colonies are not easy to grow, therefore have good identification effect, and escherichia coli is in culture medium On can grow the ne ar for being easy to differentiate, facilitate the advantages such as counting, due to connecting culture dish using culture dish mouth end Lid, interior formed of the culture dish body are equipped with GCM108 culture chambers, and the culture medium is connected through filterings such as filter paper, filter membranes, the training The microorganism that fluid to be measured is cultivated on intracavitary culture medium is supported, the culture dish is placed in incubator, due to being inhaled with sterile needle tubing Enter the liquid detected is needed to inject liquid into culture dish with syringe needle by inlet opening, extract syringe needle, then shakes culture Ware makes liquid be evenly distributed on GCM108, and then culture dish is put into constant incubator, is taken out after cultivating 24-48h, reads Bacterial number is taken, due to can be operated in normal operating conditions, requires the profession of testing staff low, common employee's process letter Single training can be operated directly, and the utility model, which solves prior art presence, can only judge to whether there is large intestine angstrom in test sample The problem of wishing bacterium, and being unable to accurate counting, the large intestine clump count that detect in test sample can be quantified, be easy to differentiate bacterium shape by having State facilitates the good advantageous effects of advantages, application prospect such as counting.

Using the technical solution of the utility model or those skilled in the art technical solutions of the utility model inspiration Under, similar technical solution is designed, and reach above-mentioned technique effect, it is to fall into the scope of protection of the utility model.

Claims (4)

1. a kind of novel quantitative counting identification culture dish for detecting escherichia coli, which is characterized in that the culture box mouth End connection culture ware lid, the culture dish body is interior to form the culture chamber equipped with culture medium, and the culture medium is through filter paper, membrane filtration Connection.

2. a kind of novel quantitative counting identification culture dish for detecting escherichia coli according to claim 1, special Sign is that the feed pathway of the culture dish is for injecting escherichia coli.

3. a kind of novel quantitative counting identification culture dish for detecting escherichia coli according to claim 2, special Sign is, the feed pathway injection escherichia coli includes that the input of inlet opening one end is connected to and is inserted into its syringe needle, another End output is connected to culture chamber.

4. a kind of novel quantitative counting identification culture dish for detecting escherichia coli according to claim 3, special Sign is, escherichia coli is cultivated on culture medium in the culture chamber.