CN107201394A - A kind of new quantitative counting authentication method and culture medium and culture dish for being used to detect EHEC - Google Patents
A kind of new quantitative counting authentication method and culture medium and culture dish for being used to detect EHEC Download PDFInfo
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- CN107201394A CN107201394A CN201710598453.2A CN201710598453A CN107201394A CN 107201394 A CN107201394 A CN 107201394A CN 201710598453 A CN201710598453 A CN 201710598453A CN 107201394 A CN107201394 A CN 107201394A
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- 239000001963 growth medium Substances 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 38
- 238000012360 testing method Methods 0.000 claims abstract description 73
- 241000894006 Bacteria Species 0.000 claims abstract description 34
- 239000000126 substance Substances 0.000 claims abstract description 24
- 238000002474 experimental method Methods 0.000 claims abstract description 15
- 239000006152 selective media Substances 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims description 19
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 11
- 241000283690 Bos taurus Species 0.000 claims description 11
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 11
- 210000000941 bile Anatomy 0.000 claims description 11
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 claims description 11
- 239000008101 lactose Substances 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 6
- 239000012895 dilution Substances 0.000 claims description 6
- 239000002207 metabolite Substances 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 6
- 238000010971 suitability test Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 7
- 210000002429 large intestine Anatomy 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 230000029052 metamorphosis Effects 0.000 description 5
- 238000012797 qualification Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/245—Escherichia (G)
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Abstract
The invention belongs to bacterium colony quantitative counting identification technology field, more particularly to a kind of new quantitative counting authentication method for being used to detect EHEC, the present invention also provides a kind of new quantitative counting identification selective medium for being used to detect EHEC simultaneously, qualitatively judges：Judge that the culture medium through culture experiment whether there is colony growth, if being not present, judge that test sample does not detect EHEC, if in the presence of according to specific chemical change, it is EHEC to judge bacterium colony；Rational judgment：The bacterium colony of single generation is further visually counted, the present invention, which solves prior art presence, can only judge to whether there is EHEC in test sample, and the problem of be unable to accurate counting, with that can quantify the large intestine clump count that detect in test sample, be easy to the ne ar of resolution, facilitate the good advantageous effects of advantage, application prospect such as counting.
Description
Technical field
The invention belongs to bacterium colony quantitative counting identification technology field, more particularly to one kind are new for detecting EHEC
Quantitative counting authentication method.
Background technology
At present, the detection of EHEC is the legal detection project of the inspections such as food, medicine, water quality, in national standard and medicine
There are strict test condition and method of inspection requirement in allusion quotation,《Chinese Pharmacopoeia》Version in 2015（The 1106 non-sterile micro- lifes of product
Thing limit test：Control bacteria examination method）Provide that the culture medium that uses is Mai Kangkai fluid nutrient mediums or maconkey agar culture medium,
《Chinese Pharmacopoeia》Detection method as defined in versions in 2015：Prepared by test liquid and Zengjing Granule takes test sample, according to " non-sterile product is micro-
Biological limit test：Microorganism count method（General rule 1105）" it is made 1：10 test liquids, take equivalent to 1g or 1ml test samples
Test liquid, is seeded to appropriate volume（Determined through method employment and suitability test (E ＆ ST)）Pancreas junket soya peptone fluid nutrient medium in, mix, 30~
35 DEG C are cultivated 18~24 hours, are selected and are separately cultured and take above-mentioned culture 1ml to be seeded to 100ml Mai Kangkai fluid nutrient mediums
In, 42~44 DEG C are cultivated 24~48 hours.The streak inoculation of Mai Kangkai liquid cultures is taken on maconkey agar culture medium flat plate,
30~35 DEG C are cultivated 18~72 hours, if as a result judging to have colony growth on maconkey agar culture medium flat plate, should be divided
From, purifying and suitable qualification test, confirmation whether be EHEC；If there is no bacterium colony on maconkey agar culture medium flat plate
Growth, though or have colony growth qualification result for feminine gender, sentence test sample and do not detect EHEC.Prior art is present can only
The problem of judging to whether there is EHEC in test sample, and be unable to accurate counting.
The content of the invention
The present invention provides a kind of new quantitative counting authentication method for being used to detect EHEC, to solve above-mentioned background
Prior art presence is proposed in technology can only judge to whether there is EHEC in test sample, and be unable to asking for accurate counting
Topic.
Technical problem solved by the invention is realized using following technical scheme：It is a kind of new for detecting that large intestine angstrom is wished
The quantitative counting authentication method of bacterium, methods described includes：
Prepare 1：The test liquid of 10 test samples；
Take 2ml test liquids to be directly seeded on culture medium GCM108 and make culture experiment；
Qualitatively judge：Judge that the culture medium through culture experiment whether there is colony growth, if being not present, judge that test sample is not examined
Go out EHEC, if in the presence of according to specific chemical change, you can judge the bacterium colony for EHEC；
Rational judgment：Naked eyes colony counting directly can be carried out to EHEC.
Further, the quantitative counting authentication method of the detection EHEC needs the test sample of same dilution level simultaneously
Two parallel tests are done, and do blank control simultaneously.
Further, the specific chemical change includes the special chemical thing that culture medium is produced to the metabolite of microorganism
Matter and the specific chemical change produced.
Further, it is described judge bacterium colony whether be EHEC include observe by the naked eye bacterium colony such as color, size and
Metamorphosis and judge it is EHEC.
Further, the culture medium is by groups such as peptone, bovine bile, lactose, bromocresol purple, filter membrane, filter paper, culture dishes
Into.
Further, the culture medium has specificity to EHEC.
Further, the culture medium is determined through method employment and suitability test (E ＆ ST).
Further, the culture medium that the culture experiment includes being inoculated with is shaken up to complete filtration, and 37 DEG C are cultivated 24~48 hours.
Meanwhile, the present invention also provides a kind of new quantitative counting identification culture medium for being used to detect EHEC, described
Culture medium is made up of peptone, bovine bile, lactose, bromocresol purple etc..
Meanwhile, the present invention provides a kind of new quantitative counting identification culture dish for being used to detect EHEC again, described
Culture dish includes the culture medium being made up of peptone, bovine bile, lactose, bromocresol purple etc., and the culture medium is through filter paper, filter membrane etc.
Filtering.
Advantageous effects：
1st, this patent is using qualitative judgement：Judge that the culture medium through culture experiment whether there is colony growth, if being not present, sentence
Determine test sample and do not detect EHEC, if in the presence of according to specific chemical change, you can judge that the bacterium colony is uncommon for large intestine angstrom
Bacterium：And the single bacterium colony of single generation is visually counted, due to being prepared by test liquid：Test sample is taken, 1 is made：10 supply
Test solution.The test liquid of 2ml test samples is taken, is directly seeded to（Determined through method employment and suitability test (E ＆ ST)）In culture medium, shake up to complete
Filtration, 37 DEG C are cultivated 24~48 hours, are as a result judged：If there is colony growth on culture medium, according to the culture medium to microorganism
The special chemical material that produces of metabolite and the specific chemical change that produces, and according to colony colour is visually observed, greatly
Small and metamorphosis and judge as EHEC；And can be visually counted from the single bacterium colony of single generation, if should
There is no colony growth on culture medium, then qualification result is feminine gender, and then judge that test sample does not detect EHEC.By to training
The chemical change combination existing method and technology of base are supported, the present invention not only realizes the qualitative judgement of test sample, and realizes
The rational judgment of test sample, the present invention can be used for the identification of EHEC, can quantify the large intestine bacterium colony detected in test sample
Number.
2nd, this patent uses the culture medium for by peptone, bovine bile, lactose, bromocresol purple, filter membrane, filter paper, culture
Ware etc. is constituted, and the culture medium has specificity to EHEC.Because the culture medium has very strong selectivity, just for
The detection of EHEC, other bacterium colonies are difficult growth, therefore are acted on good identification, and EHEC is in culture
The ne ar for being easy to differentiate can be grown on base, conveniently the advantage such as counting.
3rd, this patent using it is described detection EHEC quantitative counting authentication method need it is same dilution level for examination
Product do two parallel tests simultaneously, and do blank control simultaneously, and due to identifying and counting batch cultur, whole requirement of experiment is same
The test sample of one dilution level does two parallel tests simultaneously, and does blank control simultaneously, and parallel laboratory test is examination test method
And the accuracy of operation, contingency result is excluded, error is reduced, makes result more true and reliable；And blank control is generally added without
Sample, is mainly to weigh the sterility of culture medium, if having bacterium.
4th, this patent is suitable to the detection application such as medicine, food, water quality, application prospect well, with significant social benefit,
Economic benefit.
5th, this patent this product has detection accurate, the quick and high advantage of sensitivity.
Brief description of the drawings
Fig. 1 is a kind of new flow chart for being used to detect the quantitative counting authentication method of EHEC of the present invention.
Embodiment
The present invention is described further below in conjunction with accompanying drawing：
In figure：
S1- prepares 1：The test liquid of 10 test samples；
S2- takes 2ml test liquids to be directly seeded on GCM108 culture mediums and tested.
S3- is qualitatively judged；
S4- rational judgments.
Embodiment：
The present embodiment：As shown in figure 1, a kind of new quantitative counting authentication method for being used to detect EHEC, methods described
Including：
Prepare 1：The test liquid S1 of 10 test samples；
Take 2ml test liquids to be directly seeded on GCM108 culture mediums and make culture experiment S2；
Qualitatively judge S3：Judge that the culture medium through culture experiment whether there is colony growth, if being not present, judge test sample not
EHEC is detected, if in the presence of according to specific chemical change, you can judge bacterium colony for EHEC；
Rational judgment S4：If EHEC, then the single bacterium colony to single generation is visually counted.
Due to using qualitative judgement：Judge that the culture medium through culture experiment whether there is colony growth, if being not present, sentence
Determine test sample and do not detect EHEC, if in the presence of, according to specific chemical change, it is EHEC to judge bacterium colony,
Rational judgment：Naked eyes colony counting directly is carried out to EHEC, due to being prepared by test liquid：Test sample is taken, 1 is made：
10 test liquids.The test liquid of 2ml test samples is taken, is directly seeded to（Determined through method employment and suitability test (E ＆ ST)）In culture medium, shake up to
Filtration completely, 37 DEG C are cultivated 24~48 hours, are as a result judged：If there is colony growth on culture medium, according to the culture medium to micro-
Special chemical material that biological metabolite is produced and the specific chemical change produced, and according to visually observing bacterium colony face
Color, size and metamorphosis and be determined as EHEC；And can be visually counted from the single bacterium colony of single generation, if
There is no colony growth on the culture medium, then qualification result is feminine gender, and then judge that test sample does not detect EHEC.By right
The chemical change combination existing method and technology of culture medium, the present invention not only realize the qualitative judgement of test sample, and realize
The rational judgment of test sample, the present invention can be used for the identification of EHEC, can quantify the coliform that detect in test sample
Fall number.
The quantitative counting authentication method of the detection EHEC needs the test sample of same dilution level to do two simultaneously
Parallel test, and blank control is done simultaneously.
Because the quantitative counting authentication method using the detection EHEC needs the test sample of same dilution level same
When do two parallel tests, and blank control is done simultaneously, due to identifying and counting the whole requirement of experiment of batch cultur same
The test sample for diluting level does two parallel tests simultaneously, and does blank control simultaneously, and parallel laboratory test is examination test method and behaviour
The accuracy of work, excludes contingency result, reduces error, makes result more true and reliable；And blank control is generally added without sample,
It is mainly to weigh the sterility of culture medium, if having bacterium.
The specific chemical change includes culture medium and the special chemical material that the metabolite of microorganism is produced is produced
Raw specific chemical change.
It is described to judge whether bacterium colony is that EHEC includes observing by the naked eye bacterium colony such as color, size and metamorphosis
And determine whether EHEC.
The culture medium is to be made up of peptone, bovine bile, lactose, bromocresol purple, filter membrane, filter paper, culture dish etc..
Due to there is specificity to EHEC using the GCM108 culture mediums.Because the culture medium is with very strong
Selectivity, just for the detection of EHEC, other bacterium colonies are difficult growth, therefore are acted on good identification, and greatly
The uncommon bacterium of intestines angstrom can grow the ne ar for being easy to differentiate on culture medium, conveniently the advantage such as counting.
The culture medium has specificity to EHEC.
The culture medium is determined through method employment and suitability test (E ＆ ST).
The culture medium that the culture experiment includes inoculation is shaken up to complete filtration, and 37 DEG C are cultivated 24~48 hours.
Meanwhile, the present invention also provides a kind of new quantitative counting identification culture medium for being used to detect EHEC, described
Culture medium is made up of peptone, bovine bile, lactose, bromocresol purple etc..
Meanwhile, the present invention provides a kind of new quantitative counting identification culture dish for being used to detect EHEC again, described
Culture dish includes the culture medium being made up of peptone, bovine bile, lactose, bromocresol purple etc., and the culture medium is through filter paper, filter membrane etc.
Filtering.
Operation principle：
This patent is by using qualitative judgement：Judge that the culture medium through culture experiment whether there is colony growth, if being not present,
Then judge that test sample does not detect EHEC, if in the presence of according to specific chemical change, you can judge bacterium colony for large intestine angstrom
Uncommon bacterium, rational judgment：If EHEC, then the single bacterium colony to single generation is visually counted, due to by for trying
It is prepared by liquid：Test sample is taken, 1 is made：10 test liquids.The test liquid of 2ml test samples is taken, is directly seeded to（Tried through method applicability
Test determination）In culture medium, shake up to complete filtration, 37 DEG C are cultivated 24~48 hours, are as a result judged：If there is bacterium colony on culture medium
Growth, the then specific chemistry produced according to the culture medium to the special chemical material that the metabolite of microorganism is produced becomes
Change, and be determined as EHEC according to colony colour, size and metamorphosis is visually observed；And can be from single generation
Single bacterium colony is visually counted, if not having colony growth on the culture medium, and qualification result is feminine gender, and then judges test sample
EHEC is not detected.By the chemical change combination existing method and technology to culture medium, the present invention not only realizes confession
The qualitative judgement of test product, and the rational judgment of test sample is realized, the present invention, which solves prior art presence, can only judge confession
It whether there is EHEC in test product, and the problem of be unable to accurate counting, with the large intestine that detect in test sample can be quantified
Clump count, the ne ar for being easy to resolution, facilitate the good advantageous effects of advantage, application prospect such as counting.
Using technical scheme, or those skilled in the art is under the inspiration of technical solution of the present invention, design
Go out similar technical scheme, and reach above-mentioned technique effect, be to fall into protection scope of the present invention.
Claims (10)
1. a kind of new quantitative counting authentication method for being used to detect EHEC, it is characterised in that methods described includes：
Prepare 1：The test liquid of 10 test samples；
2ml test liquids are taken directly to be seeded to the culture medium GCM108 realities being made up of peptone, bovine bile, lactose, bromocresol purple etc.
Test；
Qualitatively judge：Judge that the culture medium through culture experiment whether there is colony growth, if being not present, judge that test sample is not examined
Go out EHEC, if in the presence of according to specific chemical change, it is EHEC to judge bacterium colony；Rational judgment：To single
The bacterium colony of generation is further visually counted.
2. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 1, its feature
Be, it is described detection EHEC quantitative counting authentication method need it is same dilution level test sample simultaneously do two it is parallel
Experiment, and blank control is done simultaneously.
3. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 1, its feature
It is, the specific chemical change includes culture medium and the special chemical material that the metabolite of microorganism is produced is produced
Specific chemical change.
4. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 1, its feature
It is, the culture medium belongs to selective medium, EHEC can be determined as by having observed by the naked eye colony growth.
5. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 1, its feature
It is, the culture medium GCM108 is made up of peptone, bovine bile, lactose, bromocresol purple, filter membrane, filter paper, culture dish etc..
6. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 1, its feature
It is, the culture medium has specificity to EHEC.
7. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 6, its feature
It is, the culture medium is determined through method employment and suitability test (E ＆ ST).
8. a kind of new quantitative counting authentication method for being used to detect EHEC according to claim 1, its feature
It is, the culture medium that the culture experiment includes inoculation is shaken up to complete filtration, and 37 DEG C are cultivated 24~48 hours.
9. it is a kind of it is new be used for detect EHEC quantitative counting identification culture medium, it is characterised in that the culture medium by
Peptone, bovine bile, lactose, bromocresol purple etc. are constituted.
10. a kind of new quantitative counting identification culture dish for being used to detect EHEC, it is characterised in that the culture dish bag
The culture medium being made up of peptone, bovine bile, lactose, bromocresol purple etc. is included, the culture medium is filtered through filter paper, filter membrane etc..
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN103642891A (en) * | 2013-11-29 | 2014-03-19 | 中山鼎晟生物科技有限公司 | Method for inspecting microbes in cosmetics |
| CN106906274A (en) * | 2017-04-06 | 2017-06-30 | 广西壮族自治区梧州食品药品检验所 | The ancestor that spreads out educates microbial limit tests in sub- capsule |
-
2017
- 2017-07-21 CN CN201710598453.2A patent/CN107201394A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614453A (en) * | 2013-05-16 | 2014-03-05 | 黑龙江中医药大学 | Gastrointestinal bacteria detection method for medical microbiology experiment |
| CN103642891A (en) * | 2013-11-29 | 2014-03-19 | 中山鼎晟生物科技有限公司 | Method for inspecting microbes in cosmetics |
| CN106906274A (en) * | 2017-04-06 | 2017-06-30 | 广西壮族自治区梧州食品药品检验所 | The ancestor that spreads out educates microbial limit tests in sub- capsule |
Non-Patent Citations (1)
| Title |
|---|
| 王文洁等: "《药物检测技术》", 中国医药科技出版社 * |
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Application publication date: 20170926 |