CN86100926A - Serial carrier type bacterial culture medium and preparation thereof - Google Patents
Serial carrier type bacterial culture medium and preparation thereof Download PDFInfo
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- CN86100926A CN86100926A CN 86100926 CN86100926A CN86100926A CN 86100926 A CN86100926 A CN 86100926A CN 86100926 CN86100926 CN 86100926 CN 86100926 A CN86100926 A CN 86100926A CN 86100926 A CN86100926 A CN 86100926A
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Abstract
Scraps of paper formula bacteria culture medium and preparation method thereof the present invention relates to bacteria culture medium and preparation thereof.These scraps of paper formula bacteria culture medium is to be carrier with filter paper, after fully absorbing the nutrient solution that contains bacterial growth nutrient, bacterial growth product indicator, assorted bacterium fungistat etc., and drying, bonding millipore filtration, encapsulation, ultraviolet disinfection is made.By selection and dosage control, can prepare the substratum that is suitable for various different bacterium cultivations to mentioned reagent.This product is without agar, have easy preparation, cost low, be easy to preserve, easy to use, good selective.
Description
The present invention relates to bacteria culture medium and preparation method thereof.In the past, bacteria culture medium all was to be matrix with agar, adds the indicator of the required nutrition agent of some bacterial growth, bacterial growth product again and the bacterium fungistat beyond the required bacterium etc. is mixed and made into.Because this classical way can be regulated by the selection and the dosage of nutrition agent and bacterium fungistat, prepares the substratum that is suitable for cultivating various bacteriums, has selectivity and practicality preferably widely.So year did not change so far surplus this classical substratum and preparation method thereof had continued to use 50.
But indubitable, this classical way still has its weak point, and the present is listed below:
One, because bacteria culture medium interim preparation when being culturing bacterium prepares substratum and all must carry out weighing reagent at every turn, be dissolved in water, regulate PH, autoclaving, the packing plate, series of complex operations such as cooled and solidified, quite time-consuming, take a lot of work.
Two, owing to when the preparation substratum, no matter do seldom several or a lot culture experiments, all need to experience whole operation process, so when using a spot of substratum, the waste of reagent and manpower is appreciable.
Though three remaining or previously prepared liquid mediums can be kept in the refrigerator, the shelf time can not be oversize, because in a single day long bacterium just can not be used again; In addition, preservation takes up space very much, influences the utilization ratio of refrigerator, is difficult to overcome waste.
Four, can only be cast in the plate because of substratum or be stored in the Glass Containers such as Erlenmeyer flask, be not easy to move, damaged easily again.
Five, with the substratum in the Erlenmeyer flask time, must use heating in water bath 1～2 hour, substratum is melted, the plate that reinjects is treated to re-use after it solidifies, and is more time-consuming.
The objective of the invention is to: provide a kind of easy to prepare, price is lower, easy preservation, easy to use, serial scraps of paper formula substratum that effect is good and preparation method thereof.This serial scraps of paper formula substratum has identical effect with corresponding nutrient agar (for example: bouillon agar, meat soup horse serum agar, SS agar, eosin methylene blue agar, high salt agar etc.).
The preparation of scraps of paper formula bacteria culture medium:
Make inoculum with the bacterium fungistat beyond bacterial growth nutrient, bacterial product indicator and required (cultivation) bacterium and (be suitable for the nutrient solution that various different bacterium are cultivated, can prepare by selection and dosage control above-mentioned nutrition agent, fungistat etc.), make aseptic culture fluid through autoclaving; Get special neutral filter paper (about 500g/m
2) be cut into desired shape and the size the scraps of paper (being generally the disk of φ 50～90mm), through autoclaving (15 pounds, 20 minutes), these scraps of paper are immersed the aseptic culture fluid made from mentioned reagent, make the scraps of paper suction nutrient solution, taking-up is spread out, be lower than under 80 ℃ the temperature dryly, making " scraps of paper " that contain nutrient solution; In sterilisable chamber, be coated in drying " scraps of paper " edge with the chemical paste that does not influence bacterial growth or other tackiness agent, make tackiness agent become the annular lamina that width is 1～2mm, again (this film can be used the soluble cotton ester less than the millipore filtration of 0.45 μ with above-mentioned " scraps of paper " onesize aperture, acetyl cellulose or mixed cellulose ester, the resilient cellulose materials of also available quality densification, as: after making such as paper) overlapping and glue (note: before bonding must involutory two sides ultraviolet disinfection) drying with above-mentioned " scraps of paper ", pack into plastics bag and sealing, use the ultraviolet irradiation two sides again, sterilization back product " scraps of paper formula bacteria culture medium ".Anti-dried, the moisture-proof, heat-resisting, cold-resistant of this product can be preserved the long period.Also need point out: (1) all nutrient agars that use at present can be made " scraps of paper formula substratum " with aforesaid method; (2) basal culture medium can be without agar, and other composition is substantially the same with classical nutrient agar; (3) also available other porous material of the above-mentioned scraps of paper replaces.
In order to improve the observing effect of inspection bacterium test, when the preparation substratum, can in nutrient solution, add the bacterium staining agent, perhaps achieve the goal by dosage control; For using some extraordinary substratum easily, must take special process in the preparation.For example:
When preparation ordinary broth or meat soup serum scraps of paper formula substratum, 0.1%TTC(2,3, the 5-triphenyltetrazolium chloride that can in nutrient solution, add 1/100 nutrient solution volume) the bacterium staining agent, the bacterial colony of growth is taken on a red color, more apparent distinctness is easy to EARLY RECOGNITION under the setoff of the white scraps of paper.
During preparation Yihong U.S. blue scraps of paper formula substratum, reagent Yihong and Mei Lan solution dosage routinely double.Like this, make the coliform of growth be red-purple, be easy to observe and EARLY RECOGNITION.
When preparing the blood scraps of paper formula substratum suitable with blood agar, can be meat soup and defiber sheep (or rabbit) blood with 100: 5～10 mixed, add from the millipore filtration face then, until saturated (preferably after amount), make face form the film of blood, easy to use, add nutrient solution amount must the appropriateness.
The using method of basal culture medium:
Cut off plastics bag, with the aseptic nipper gripping scraps of paper, put into aseptic plate (note: filter membrane is towards last), add sterile distilled water, make the scraps of paper drenched to saturated from sheet edge, incline and surplus water in the plate, do streak inoculation with experiment material (for example: the bacterium of going down to posterity, cultivation phlegm, ight soil, purulence, urine or other secretory product etc.), build plate, turn over to be buckled in the incubator and cultivate, observe bacterium colony next day.(if plate is of poor quality and can not be airtight, behind the back-off, adds 1～2mL sterile distilled water in loam cake, to keep humidity in the ware).
During inoculation, because of in addition water be difficult to be adsorbed on the complete dissolved of reagent on the scraps of paper, can influence the substratum composition, in order to improve culture condition, all ingredients dosage of preparation scraps of paper formula substratum preferably will suitably increase (in case of necessity, multiplicable).
The present invention has also prepared the meat soup scraps of paper that replace meat soup according to the needs of sewage or urine inspection bacterium.Method for making is as follows: preparation earlier concentrates meat soup, and measures its concentration and cumulative volume, adds the scraps of paper of a certain amount of (area or number), and the scraps of paper are suctioned meat soup, again with scraps of paper drying, contain the meat soup scraps of paper of quantitative meat soup.During use, only need the meat soup amount that contains, calculate the meat soup scraps of paper amount (as number) that needs, it is dropped in the material of bacterium to be checked, use very convenient according to consumption and every or the unit surface scraps of paper.(for example: absorb if the meat soup of W. gram is opened the scraps of paper by N., then every paper contains meat soup (W.)/(N.) gram, and need use W now
1Gram meat soup, the number N of the then required meat soup scraps of paper
1=(W
1)/(W.) opened).
These scraps of paper formula substratum also can be used for the sensitization test of microbiotic to bacterium, and method is identical with conventional method, and the judgement of antibacterial ring is also identical with conventional method.When if scraps of paper formula substratum contains aforesaid TTC, antibacterial ring can occur in 5～6 hours after inoculation, helps early diagnosis and therapy.
Embodiment:
I had once prepared a considerable amount of scraps of paper formula substratum by above-mentioned preparation method, wherein had to contain two series agar and that do not contain agar, and through facts have proved of culturing bacterium, the both has identical effect.Preparation experiment shows, not only cost is lower not contain the scraps of paper formula substratum of agar, and operation also makes things convenient for manyly.I will not contain the scraps of paper formula substratum of agar and give hospital evaluation on probation, and the cultivation of a lot of bacterial classifications of process and tested material (as: phlegm, excrement, throat swab etc.) confirms: the culture effect of scraps of paper formula substratum is tantamount to the nutrient agar of classical approach preparation really.
Claims (3)
1, bacteria culture medium in the past all is to be matrix with agar, add bacterial growth nutrient again, fungistat beyond bacterial growth product indicator and the required bacterium is mixed and made into, the nutrient agar that is suitable for various different bacterium cultivations can be regulated acquisition by selection and dosage to mentioned reagent, the invention is characterized in: (a) carrier type bacterial culture medium is to use porous material, millipore filtration and contain bacterial growth nutrient, the inoculum of the fungistat beyond bacterial growth product indicator and the required bacterium is made, and is applicable to that (b) carrier type bacterial culture medium of different bacterium cultivation uses the inoculum that does not contain agar that the selection and the dosage of mentioned reagent are regulated acquisition to make; (c) some carrier type substratum (as, meat soup or meat soup blood serum medium, blood meida, the U.S. blue substratum of Yi Jiang etc.) can be respectively by the effect that adds the bacterium staining agent, blood (clearly) is handled and at double dosage adjusting acquisition be easy to discern.
2, carrier type bacterial culture medium according to claim 1, its preparation method is characterised in that: carrier type bacterial culture medium is after having absorbed the inoculum of preparation with porous material, make through super-dry, bonding millipore filtration, encapsulation, ultraviolet disinfection again, above-mentioned materials and reagent all are that operation is a sterile procedures through autoclaved.
3, carrier type bacterial culture medium according to claim 1, it is characterized in that: described millipore filtration can use soluble cotton ester, acetyl cellulose, mixed cellulose ester or other quality densification whippy cellulose materials (as, paper etc.) making, is good with micropore size less than 0.45 material wherein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86100926 CN86100926A (en) | 1986-02-06 | 1986-02-06 | Serial carrier type bacterial culture medium and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86100926 CN86100926A (en) | 1986-02-06 | 1986-02-06 | Serial carrier type bacterial culture medium and preparation thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN86100926A true CN86100926A (en) | 1987-08-26 |
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ID=4801250
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 86100926 Pending CN86100926A (en) | 1986-02-06 | 1986-02-06 | Serial carrier type bacterial culture medium and preparation thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104395457A (en) * | 2012-02-02 | 2015-03-04 | 康宁股份有限公司 | Novel strain producing d-lactic acid and use thereof |
| CN104911243A (en) * | 2015-06-29 | 2015-09-16 | 汇征联合（北京）医疗器械有限公司 | Solid culture medium for inoculating liquid samples and culture method |
| CN106282299A (en) * | 2015-05-14 | 2017-01-04 | 北京中医药大学 | A kind of method of external artificial acquisition bacterial resistance bacterial strain |
| CN107090401A (en) * | 2017-06-09 | 2017-08-25 | 天津施特雷生物科技股份有限公司 | A kind of drying disposable sterilized microbial culture dish |
-
1986
- 1986-02-06 CN CN 86100926 patent/CN86100926A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104395457A (en) * | 2012-02-02 | 2015-03-04 | 康宁股份有限公司 | Novel strain producing d-lactic acid and use thereof |
| CN104395457B (en) * | 2012-02-02 | 2018-02-27 | 康宁股份有限公司 | Synthesis for cell culture adheres to culture medium |
| US10400210B2 (en) | 2012-02-02 | 2019-09-03 | Corning Incorporated | Synthetic attachment medium for cell culture |
| US11718824B2 (en) | 2012-02-02 | 2023-08-08 | Corning Incorporated | Synthetic attachment medium for cell culture |
| CN106282299A (en) * | 2015-05-14 | 2017-01-04 | 北京中医药大学 | A kind of method of external artificial acquisition bacterial resistance bacterial strain |
| CN104911243A (en) * | 2015-06-29 | 2015-09-16 | 汇征联合（北京）医疗器械有限公司 | Solid culture medium for inoculating liquid samples and culture method |
| CN107090401A (en) * | 2017-06-09 | 2017-08-25 | 天津施特雷生物科技股份有限公司 | A kind of drying disposable sterilized microbial culture dish |
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