CN202246686U - Centrifugal blood culture bottle - Google Patents
Centrifugal blood culture bottle Download PDFInfo
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- CN202246686U CN202246686U CN201120250855.1U CN201120250855U CN202246686U CN 202246686 U CN202246686 U CN 202246686U CN 201120250855 U CN201120250855 U CN 201120250855U CN 202246686 U CN202246686 U CN 202246686U
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- bottle
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- centrifugal
- centrifugal blood
- blood culture
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- 238000009640 blood culture Methods 0.000 title claims abstract description 22
- 239000007799 cork Substances 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 6
- 229930182490 saponin Natural products 0.000 abstract description 6
- 150000007949 saponins Chemical class 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 abstract description 4
- 239000011521 glass Substances 0.000 abstract description 3
- 229920003023 plastic Polymers 0.000 abstract description 3
- 239000004033 plastic Substances 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 2
- 229920001463 polyanetholesulfonic acid sodium salt Polymers 0.000 abstract 2
- 230000000813 microbial effect Effects 0.000 abstract 1
- 229920001451 polypropylene glycol Polymers 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 16
- 210000004369 blood Anatomy 0.000 description 15
- 239000008280 blood Substances 0.000 description 15
- 238000011081 inoculation Methods 0.000 description 10
- 239000007788 liquid Substances 0.000 description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 241000588653 Neisseria Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000010241 blood sampling Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- -1 methyl allylphenol Chemical compound 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 2
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- 241000191992 Peptostreptococcus Species 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 206010046306 Upper respiratory tract infection Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000937 inactivator Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 235000001892 vitamin D2 Nutrition 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a centrifugal blood culture bottle and provides a clinical microbial detection and culture device. The centrifugal blood culture bottle comprises a sealed bottle cover, an inner cover and a centrifugal tube, as well as a culture solution in the tube, wherein the sealed bottle cover is used for wrapping a bottle mouth of the whole centrifugal blood culture bottle; the inner cover is a cork and has a sealing effect; the centrifugal tube is taken as a bottle body of the culture bottle and is made of a glass or plastic material; and the culture solution stays at the bottom in the tube and contains sodium polyanetholesulfonate (SPS), sodium thioglycollate, saponin and polypropylene glycol. The centrifugal blood culture bottle disclosed by the utility model is simple in structure and convenient to use, and can be used for simultaneously performing culture and purification so as to shorten culture time, and simultaneously performing counting so as to provide valuable clinical information.
Description
Technical field
The utility model relates to medical detection and uses device, is specially a kind of centrifugal Blood culture bottle.
Background technology
At present; The instrument that is used for blood cultivation clinically is more complicated all; Mainly contain the BACTEC 9000 Automatic Blood Culture System of U.S. BD, the Bact/ALERT 3D Automatic Bacteria Culture System of French Mei Liai, they all are the outer continuously automatic surveillance and monitoring systems of the auxiliary bottle of liquid nutrient medium; Its advantage is that level of automation is high, simple to operate.Its shortcoming is behind the newspaper sun, must be passaged to cultivate on the solid medium more than 24 hours again, has prolonged incubation time again, if contaminated accidentally when blood sampling, contaminated bacteria will disturb the recovery of pathogenic bacterium when increasing bacterium.The ISOLATOR Blood Culture System that also has Britain OXOID in addition, it is in a sterilization centrifuge tube, to contain SUNLECITHIN A, saponin; Longer chain fatty acid, buffer reagent, materials such as microbiotic inactivator; In 10ml blood injecting tube, mixing is centrifugal after hatching and letting hemocyte dissolve fully, abandons supernatant; Get sediment direct inoculation solid medium or change over to increase bacterium in the liquid enrichment medium after, reach again on the solid medium, with the naked eye observe obtaining bacterial colony at an easy rate.Its advantage is: price is low, safeguards easily, is easy to popularize use in China; Simple to operate, practicality; To dysgonic bacterium in fungi and the liquid medium within (like hemophilus influenza, meningococcus, mycobacterium etc.), can obviously improve positive rate; When cultivating, obtain dividing pure bacterium colony, shortened incubation time.Its shortcoming is: owing to be normal pressure in the bottle, need use blood collection needle, need be after centrifugal with capping machine many steps of how having added a cover the centre, and the chance of pollution has just increased, and influences blood anticoagulant, cracking.This shows that all there is certain shortcoming in the blood cultivation instrument that uses at present, need further to improve.
The utility model content
The technical problem that the utility model solved is to provide a kind of centrifugal Blood culture bottle, to solve the shortcoming in the above-mentioned background technology.
The technical problem that the utility model solved adopts following technical scheme to realize:
Centrifugal Blood culture bottle comprises seal bottle cap, inner cap and centrifuge tube, and the nutrient solution in the pipe.Wherein, Seal bottle cap encases the bottleneck of whole centrifugal Blood culture bottle, and inner cap is a cork, plays the effect of sealing; Centrifuge tube is the body of culturing bottle; Be glass or plastic material, nutrient solution rests on the bottom in the pipe, and the methyl allylphenol of gathering sodium sulfonate (SPS), Thioglycolic acid sodium salt, saponin and W 166 are arranged in it.
Have negative pressure in the said centrifugal Blood culture bottle, blood sample can directly be inhaled in the bottle in the time of blood sampling, simplifies the operation, and reduces and pollutes.For the nutrient solution in the bottle: saponin is used for splitting erythrocyte and white corpuscle, can intracellular thalline be discharged, and also discharges the nutritive ingredient in the born of the same parents simultaneously; W 166 is used to eliminate the foam that saponin produces; SPS is used to prevent blood aggegation and incrustation, suppresses white corpuscle, and antibody protein and N,O-Diacetylmuramidase suppress antibiotic activity to the destruction of thalline, and the oxyphorase that discharges can be protected Neisseria and the peptostreptococcus responsive to SPS; Thioglycolic acid sodium salt is used to provide anaerobic environment.This nutrient solution cleavable blood lets thalline fully discharge.Because generally speaking, particularly after using microbiotic, the cell concentration＜1CFU/ml in the blood is so the centrifugal thalline that makes concentrates.
The centrifugal Blood culture bottle of the utility model is a sealed structure, can go after centrifugal lid to abandon supernatant, and also available suction pipe sucking-off need not to add a cover again.At last with the liquid concentrator direct inoculation on conventional agar plate, single bacterium colony is obtained in the line of utilization chessboard strok method.
Beneficial effect: the utility model is simple in structure, and is easy to use, will cultivate and purify and carry out simultaneously, shortens incubation time, can count simultaneously, for clinical valuable information is provided.
Description of drawings
Fig. 1 is the structural representation of the utility model preferred embodiment.
Among the figure: seal bottle cap 1, inner cap 2, centrifuge tube 3, nutrient solution 4.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach purpose and be easy to understand understanding with effect, below in conjunction with concrete diagram, further set forth the utility model.
Centrifugal hemoculture liquid referring to Fig. 1 comprises seal bottle cap 1, inner cap 2 and centrifuge tube 3, and the nutrient solution 4 in the pipe.Wherein, Seal bottle cap 1 encases the bottleneck of whole centrifugal Blood culture bottle, and inner cap 2 is a cork, plays the effect of sealing; Centrifuge tube 3 is bodies of culturing bottle; Be glass or plastic material, nutrient solution 4 rests on the bottom in the pipe, and the methyl allylphenol of gathering sodium sulfonate (SPS), Thioglycolic acid sodium salt, saponin and W 166 are arranged in it.
The utility model has the strict use flow process of comparison in use:
1, collection of specimens
Adopt strict sterile procedure, adult's inoculation is 10ml blood nearly, and children's inoculation nearly [z1] ml blood is put upside down separator tube 4-5 time immediately in centrifugal Blood culture bottle, write label.The adult does not use the blood sample less than [z2] ml, children not to use the blood sample less than 0.5ml.
2, the processing of sample
1. sample should be handled to the laboratory as early as possible.Separate with centrifugal Blood culture bottle immediately, reduce blood bacteriostatic influence to greatest extent, to greatest extent separate microorganism with valuable quantitative information possibly is provided.
2. at room temperature, sample reaches 16 hours in centrifugal Blood culture bottle, can the recovery of mikrobe not had a negative impact.The patient's of antibiotic chemotherapy sample should be handled immediately, if sample surpasses 4 hours in bottle, enumeration can not reflect the colony-forming unit in every milliliter of blood sample.Will not collect the bottle refrigeration of sample, cold gonococcus may significantly reduce after recovery.
3. the content in the thorough mixing bottle.Bottle is packed in the concentrator bowl, with 3000G centrifugal 30 minutes, please don't the apply the brakes device when slowing down.
4. with suitable sterilizing agent sterilization bottle stopper, extract the bottle end with the minute hand syringe and precipitate, also direct decap abandons supernatant and gets deposition.
5. will precipitate on the adding isolation medium, each dull and stereotyped maximum 0.35ml evenly rules with the transfering loop grid, but does not run into the agar edge.
6. in order to separate severe bacteria and anerobes as far as possible,, should put into as early as possible under the suitable culture condition in case inoculated sample.
7. preceding 24 hours of aerobic flat board, uprightly after this be inverted in dull and stereotyped preceding 48 hours by all flat boards for anaerobism.
8. all flat board of daily inspection is to abandoning.Even the growth phenomenon occurs very soon, flat board should continue to cultivate, so that second kind of bacterial growth arranged after finding.The inspection flat board should be across lid.If when perhaps having colonies typical to take away lid, do not take off lid fully, just improve lid and get final product to the zone that can be checked through bacterium colony because of moisture condensation.
3, result's explanation
If 1. have only a bacterium colony to occur in the zone of inoculation, no matter belong to or kind, it should be regarded as a significant positive.And the hemoculture enumeration of paediatrics is generally high than the adult, and the microbemia that takes place behind upper respiratory tract infection or the antibacterial therapy counts that low (< 10 CFU/>ml), this is not rare.
If 2. bacterium colony appear in the inoculation district with the inoculation district outside, can consider that in the inoculation district be a positive, the inoculation district outer as contaminated bacteria.If these two bacterium colonies are identical belonging to planting, they are possible contaminated bacterias.
If 3. have only a bacterium colony, appear at outside the inoculation zone, it can be regarded as the contaminated bacteria of a flat board.
More than show and described the advantage of ultimate principle, principal character and the utility model of the utility model.The technician of the industry should understand; The utility model is not restricted to the described embodiments; The principle of describing in the foregoing description and the specification sheets that the utility model just is described; Under the prerequisite that does not break away from the utility model spirit and scope, the utility model also has various changes and modifications, and these variations and improvement all fall in the utility model scope that requires protection.The utility model requires protection domain to be defined by appending claims and equivalent thereof.
Claims (2)
1. centrifugal Blood culture bottle is characterized in that, comprises seal bottle cap, inner cap and centrifuge tube; And the nutrient solution in the pipe, wherein, seal bottle cap encases the bottleneck of whole centrifugal Blood culture bottle; Inner cap is a cork, and centrifuge tube is the body of culturing bottle, and nutrient solution rests on the bottom in the pipe.
2. centrifugal Blood culture bottle according to claim 1 is characterized in that, has negative pressure in the said centrifugal Blood culture bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120250855.1U CN202246686U (en) | 2011-07-16 | 2011-07-16 | Centrifugal blood culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120250855.1U CN202246686U (en) | 2011-07-16 | 2011-07-16 | Centrifugal blood culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202246686U true CN202246686U (en) | 2012-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201120250855.1U Expired - Fee Related CN202246686U (en) | 2011-07-16 | 2011-07-16 | Centrifugal blood culture bottle |
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| Country | Link |
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| CN (1) | CN202246686U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107811854A (en) * | 2017-09-25 | 2018-03-20 | 南方医科大学南方医院 | A kind of Blood culture bottle bottleneck |
-
2011
- 2011-07-16 CN CN201120250855.1U patent/CN202246686U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107811854A (en) * | 2017-09-25 | 2018-03-20 | 南方医科大学南方医院 | A kind of Blood culture bottle bottleneck |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20160716 |
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| CF01 | Termination of patent right due to non-payment of annual fee |