CN217149199U

CN217149199U - Automatic sampling device

Info

Publication number: CN217149199U
Application number: CN202221559077.9U
Authority: CN
Inventors: 不公告发明人
Original assignee: To Microbial Intelligent Technology Xiamen Co ltd; Zhimei Times Biological Intelligent Technology Beijing Co ltd
Current assignee: To Microbial Intelligent Technology Xiamen Co ltd; Zhimei Times Biological Intelligent Technology Beijing Co ltd
Priority date: 2022-06-22
Filing date: 2022-06-22
Publication date: 2022-08-09
Anticipated expiration: 2032-06-22

Abstract

The utility model provides an automatic change sampling device relates to microorganism sampling equipment technical field, include: the sampling device comprises a plurality of sampling tubes, sampling heads, magnetic needles, a driving device and a controller; sampling liquid and nucleic acid adsorption magnetic bead have all been placed in advance in a plurality of sampling pipes, sampling head cover locates on arbitrary one sampling pipe after all can constitute a sampling bottle, the controller can control drive arrangement drive sampling head cover and locate on arbitrary one sampling pipe, the cover is equipped with the magnetic needle sleeve pipe in advance on the magnetic needle, the controller can control drive arrangement drive magnetic needle and drive the magnetic needle sleeve pipe and stretch into arbitrary one sampling pipe and adsorb the nucleic acid in it and adsorb the magnetic bead. The utility model provides an automatic change sampling device need not manual operation, can repeatedly carry out many times air sampling and carry out quick nucleic acid to the air microorganism and draw, and then realize the purpose of short-term test.

Description

Automatic sampling device

Technical Field

The utility model relates to a microorganism sampling equipment technical field especially relates to an automatic change sampling device.

Background

Microorganisms in human, animal and plant bodies and soil can be dispersed in the air by droplets, dust, and the like, so that the air contains a certain kind and number of microorganisms. There are generally no pathogenic microorganisms in the air theoretically, but aerosols of pathogenic microorganisms are often suspended in the air near hospitals, veterinary hospitals and livestock houses, and healthy people or animals are often infected by inhalation. Air contaminated by pathogenic microorganisms can often be a source or vehicle of contamination, causing epidemic disease. Therefore, sampling and detecting the air microorganisms are of great significance for infectious disease prevention and control and environmental hygiene supervision and protection, before the microorganisms are detected, nucleic acid of the microorganisms needs to be extracted in advance, the existing air microorganism collection and nucleic acid extraction mainly depend on manual operation, the air microorganism sample collection and nucleic acid extraction are completed by manually operating different instruments and equipment, and the operation is complex, the time consumption is long, and the technical requirement is high.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an automatic change sampling device to solve the problem that above-mentioned prior art exists, the utility model provides an automatic change sampling device need not manual operation, can repeatedly carry out many times air sampling and carry out quick nucleic acid extraction to the air microorganism, and then realize the purpose of short-term test.

In order to achieve the above object, the utility model provides a following scheme:

the utility model provides an automatic change sampling device, include: the sampling device comprises a plurality of sampling tubes, sampling heads, magnetic needles, a driving device and a controller; it is a plurality of sampling liquid and nucleic acid adsorption magnetic bead have all been placed in advance in the sampling tube, the sampling head lid is located arbitrary one sampling bottle all can be constituteed after on the sampling tube, the controller can control the drive arrangement drive the sampling head lid is located arbitrary one on the sampling tube, the cover is equipped with the magnetic needle sleeve pipe in advance on the magnetic needle, the controller can control the drive arrangement drive the magnetic needle drives the magnetic needle sleeve pipe stretches into arbitrary one in the sampling tube and adsorb nucleic acid in it adsorbs the magnetic bead.

Preferably, the plurality of sampling tubes are uniformly arranged along the first straight line.

Preferably, the driving device comprises a horizontal driving assembly, a lifting driving assembly and a rotating driving assembly, the rotating driving assembly is slidably connected to a vertical slide rail of the lifting driving assembly, and the lifting driving assembly is slidably connected to a horizontal slide rail of the horizontal driving assembly;

still include the runing rest, the runing rest set up in the free end of runing drive subassembly, the runing drive subassembly can drive the runing rest rotates around a vertical axle, the magnetism needle with the sampling head all fixed set up in on the runing rest, just the magnetism needle with the central line of sampling head extremely the distance of vertical axle is the same.

Preferably, the magnetism needle stretches into the sheathed tube one end of magnetism needle is the bottom, and the other end is the top, the magnetism needle is close to be provided with on the outer wall on top along the first arch of circumference extension, be provided with on the inner wall that the magnetism needle sleeve pipe is close to the top along the recess of circumference extension, the magnetism needle sleeve pipe is made by elastic material, the magnetism needle insert locate in the magnetism needle sleeve intraductally, first arch can the joint in the recess.

Preferably, only the bottom end of the magnetic needle is magnetic.

Preferably, the sampling head includes lid, intake pipe and outlet duct, the intake pipe with the outlet duct all runs through the lid, the upper end opening and the vacuum pump intercommunication of outlet duct, the lid is located when sampling pipe is last, the lower extreme opening of intake pipe accesss to in the sampling pipe, the upper end opening of intake pipe accesss to the surrounding environment, the lower extreme opening of outlet duct accesss to in the sampling pipe and be located in the sampling pipe on the liquid level of sampling liquid.

Preferably, a rubber pad is arranged at the top end of the sampling tube and/or the bottom end of the cover body.

Preferably, the sampling solution is a lysis solution.

The utility model discloses for prior art gain following technological effect:

the utility model provides an automatic sampling device, which comprises a plurality of sampling tubes, a sampling head, a magnetic needle, a driving device and a controller; when needs are sampled the air, controller control drive arrangement drive sampling skull is located on arbitrary one sampling pipe, constitute a sampling bottle, controller control sampling bottle carries out the sampling of air microorganism, the microorganism sample of collection can be in the sampling liquid (with the lysate as the sampling liquid) schizolysis and release nucleic acid, nucleic acid is adsorbed by nucleic acid adsorption magnetic bead, then controller control drive arrangement drive magnetic needle and drive the magnetic needle sleeve pipe stretch into arbitrary one sampling pipe and adsorb the nucleic acid adsorption magnetic bead in it, and because of being provided with a plurality of sampling pipes, can repeat the process of sampling many times and nucleic acid extraction, therefore, the utility model provides an automatic sampling device need not manual operation, can repeat many times air sampling and carry out quick nucleic acid extraction to air microorganism, and then realize the purpose of quick detection.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic mechanism diagram of an automated sampling device provided by the present invention;

FIG. 2 is a schematic view of the mechanism of the magnetic needle;

FIG. 3 is a schematic structural view of a magnetic needle cannula;

FIG. 4 is a cross-sectional view of FIG. 3;

FIG. 5 is a schematic structural diagram of a sampling head and a sampling tube after combination;

FIG. 6 is a schematic view of a sampling head structure;

fig. 7 is a schematic structural diagram of the combined automatic sampling device and subsequent detection device provided by the present invention;

FIG. 8 is a top view of the internal structure of FIG. 7;

in the figure: 1. a sampling tube; 2. a magnetic needle; 21. a first protrusion; 3. a magnetic needle cannula; 31. a groove; 4. rotating the bracket; 5. a rotary drive assembly; 6. a horizontal slide rail; 7. a vertical slide rail; 8. a sampling head; 81. a cover body; 82. an air inlet pipe; 83. an air outlet pipe; 9. a placing table; 10. an operation table; 11. a horizontal drive assembly; 20. an automated sampling device; 30. and (4) a detection device.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model aims at providing an automatic change sampling device to solve the problem that prior art exists, the utility model provides an automatic change sampling device need not manual operation, can repeatedly carry out many times air sampling and carry out quick nucleic acid extraction to the air microorganism, and then realize the purpose of short-term test.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

The utility model provides an automatic change sampling device 20, as shown in fig. 1~ fig. 6, include: the device comprises a plurality of sampling tubes 1, a sampling head 8, a magnetic needle 2, a driving device and a controller; sampling liquid (sampling liquid is the lysate) and nucleic acid adsorption magnetic bead have all been placed in advance in a plurality of sampling pipes 1, sampling head 8 lid is located arbitrary sampling pipe 1 and is gone up the back homoenergetic and form a sampling bottle, preferably impact sampling bottle, the sampling bottle of constitution is arranged in sampling the microorganism in the air, the controller can control drive arrangement drive sampling head 8 lid and locate arbitrary sampling pipe 1 on, the cover is equipped with magnetism needle sleeve pipe 3 in advance on the magnetism needle 2, the controller can control drive arrangement drive magnetism needle 2 and drive magnetism needle sleeve pipe 3 and stretch into arbitrary sampling pipe 1 and adsorb the nucleic acid adsorption magnetic bead in it.

The utility model provides an automatic sampling device 20, which comprises a plurality of sampling tubes 1, a sampling head 8, a magnetic needle 2, a driving device and a controller; when air is required to be sampled, the controller controls the driving device to drive the sampling head 8 to cover any one sampling tube 1 to form a sampling bottle, the controller controls the sampling bottle to sample air microorganisms, the collected microorganism samples can be cracked in sampling liquid (cracking liquid is used as sampling liquid) and nucleic acid is released, the nucleic acid is adsorbed by nucleic acid adsorption magnetic beads, then the controller controls the driving device to drive the magnetic needle 2 and the magnetic needle sleeve 3 to extend into any one sampling tube 1 and adsorb nucleic acid adsorption magnetic beads in the sampling tube 1, and because a plurality of sampling tubes 1 are arranged, the process of sampling and nucleic acid extraction can be repeated for a plurality of times, therefore, the automatic sampling device 20 provided by the utility model does not need manual operation, repeated air sampling can be carried out for many times, and the air microorganism is subjected to rapid nucleic acid extraction, so that the purpose of rapid detection is realized.

Furthermore, in order to avoid cross contamination between samples, the magnetic needle cannula needs to be replaced before each sample.

The utility model provides an automatic change sampling device 20 can automize and accomplish the process of air microorganism sampling and nucleic acid extraction, need not manual operation, and technical requirement is low, has avoided the miscellaneous fungus pollution that loaded down with trivial details operation introduced, and convenient and fast for air microorganism's detection process. In addition, parts such as sampling pipe 1, magnetic needle sleeve pipe 3 all design as disposable consumptive material, have avoided the cross contamination that the used repeatedly arouses. The system can also be used in scenes outside a laboratory, such as families, markets, schools and the like, and has wide application range.

Further, a plurality of sampling pipes 1 evenly arrange along first straight line, the controller can control drive arrangement drive sampling head 8 and cover in proper order along first straight line and locate sampling pipe 1 on, and can control the sampling bottle of temporarily constituteing and carry out air sampling, after the sampling finishes, drive arrangement drive sampling head 8 keeps away from the sampling pipe 1 that the loading has the nucleic acid, this moment sampling pipe 1's upper shed is uncovered, and then realize that magnetic needle 2 and magnetic needle sleeve pipe 3 stretch into the purpose that adsorbs the nucleic acid in it and adsorb the magnetic bead in sampling pipe 1.

Further, the driving device comprises a horizontal driving component 11, a lifting driving component and a rotating driving component 5, wherein the rotating driving component 5 is connected to the vertical slide rail 7 of the lifting driving component in a sliding manner, and the lifting driving component is connected to the horizontal slide rail 6 of the horizontal driving component 11 in a sliding manner;

still include runing rest 4, runing rest 4 sets up in the free end of runing rest 5, runing rest 4 can be driven around a vertical rotation of axle to runing rest 5, magnetic needle 2 and sampling head 8 are all fixed to be set up on runing rest 4, and the central line of magnetic needle 2 and sampling head 8 is the same to vertical axial distance, and then can realize the process that air microorganism sampled and nucleic acid extracted through simple circular motion and linear motion, the cost of manufacture has been reduced.

Furthermore, one end of the magnetic needle 2 extending into the magnetic needle sleeve 3 is a bottom end, the other end is a top end, the outer wall of the magnetic needle 2 close to the top end is provided with two first bulges 21 extending along the circumferential direction, preferably two first bulges are arranged, the inner wall of the magnetic needle sleeve 3 close to the top end is provided with two grooves 31 extending along the circumferential direction, the magnetic needle sleeve 3 is made of elastic material, when the magnetic needle 2 is inserted into the magnetic needle sleeve 3, the first bulges 21 can be clamped in the grooves 31, the magnetic needle sleeve 3 can be slightly elastically deformed when being stressed, when the magnetic needle 2 is inserted into the magnetic needle sleeve 3, the first bulges 21 can be clamped in the grooves 31, the magnetic beads can be adsorbed after the magnetic needle sleeve 3 is sleeved on the magnetic needle 2, the magnetic needle sleeve 3 is fixed on the magnetic needle 2 without external force, and the magnetic needle sleeve 3 can be easily taken down from the magnetic needle 2 manually, the magnetic needle 2 is set to have magnetism only at the lowest end, so that the magnetic beads can only be adsorbed at the lower end of the magnetic needle sleeve 3, and the nucleic acid on the magnetic beads can be conveniently eluted subsequently.

Further, sampling head 8 includes lid 81, intake pipe 82 and outlet duct 83 all run through lid 81, the upper end opening and the vacuum pump intercommunication of outlet duct 83, when lid 81 lid was located on sampling pipe 1, the lower extreme opening of intake pipe 82 accesss to in the sampling pipe 1, the upper end opening of intake pipe 82 accesss to the surrounding environment, the lower extreme opening of outlet duct 83 accesss to in the sampling pipe 1 and be located on the liquid level of the sampling liquid in the sampling pipe 1, opening and close of controller control vacuum pump.

Further, for making the leakproofness better after sampling head 8 and sampling pipe 1 make up, can be provided with the rubber pad at the bottom of sampling pipe 1 top and/or lid 81, specifically for the cover is cyclic annular, and after the combination, the rubber pad is in by compression state, and then realizes sealed effect.

Adopt the utility model provides an automatic change sampling device 20 and carry out the work flow of air microorganism sampling:

1. adding a sampling solution and nucleic acid adsorption magnetic beads into a sampling tube 1 in advance, and sleeving a magnetic needle sleeve 3 on a magnetic needle 2;

this device uses the lysate as sampling liquid, makes the microbial sample schizolysis of gathering among the air sampling process and makes the nucleic acid absorption of microbial lysis release to nucleic acid adsorb the magnetic bead on, improves work efficiency.

2. Controlling the sampling head 8 to move above the sampling pipe 1 and descend to be combined with the sampling pipe 1 to form a sampling bottle, starting a vacuum pump, and carrying out air sampling (in the process, nucleic acid released by air microbial cracking is adsorbed onto nucleic acid adsorption magnetic beads);

3. after sampling is finished, controlling the sampling head 8 to ascend, controlling the magnetic needle 2 (sleeved with the magnetic needle sleeve 3) to move into the sampling tube 1, and adsorbing magnetic beads onto the surface of the magnetic needle sleeve 3;

as shown in fig. 7 to 8, the magnetic needle 2 can be lifted up to transfer the magnetic beads to the subsequent detection device 30 for the subsequent detection operation, and the detection device 30 is disposed on one side of the automated sampling device 20.

The utility model discloses a concrete example is applied to explain the principle and the implementation mode of the utility model, and the explanation of the above example is only used to help understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.

Claims

1. An automated sampling device, comprising: the method comprises the following steps: the sampling device comprises a plurality of sampling tubes, sampling heads, magnetic needles, a driving device and a controller; it is a plurality of sampling liquid and nucleic acid adsorption magnetic bead have all been placed in advance in the sampling tube, the sampling head lid is located arbitrary one sampling bottle all can be constituteed after on the sampling tube, the controller can control the drive arrangement drive the sampling head lid is located arbitrary one on the sampling tube, the cover is equipped with the magnetic needle sleeve pipe in advance on the magnetic needle, the controller can control the drive arrangement drive the magnetic needle drives the magnetic needle sleeve pipe stretches into arbitrary one in the sampling tube and adsorb nucleic acid in it adsorbs the magnetic bead.

2. The automated sampling device of claim 1, wherein: the plurality of sampling tubes are uniformly arranged along a first straight line.

3. The automated sampling device of claim 1, wherein: the driving device comprises a horizontal driving assembly, a lifting driving assembly and a rotary driving assembly, wherein the rotary driving assembly is connected to a vertical sliding rail of the lifting driving assembly in a sliding manner, and the lifting driving assembly is connected to a horizontal sliding rail of the horizontal driving assembly in a sliding manner;

4. The automated sampling device of claim 1, wherein: the utility model discloses a magnetic needle, including magnetic needle sleeve pipe, magnetic needle, wherein, magnetic needle, insert, magnetic needle, wherein, magnetic needle, wherein, magnetic needle, wherein, magnetic needle, wherein, magnetic needle, wherein, magnetic needle.

5. The automated sampling device of claim 1, wherein: only the bottom end of the magnetic needle is provided with magnetism.

6. The automated sampling device of claim 1, wherein: the sampling head includes lid, intake pipe and outlet duct, the intake pipe with the outlet duct all runs through the lid, the upper end opening and the vacuum pump intercommunication of outlet duct, the lid is located when on the sampling pipe, the lower extreme opening of intake pipe accesss to in the sampling pipe, the upper end opening of intake pipe accesss to the surrounding environment, the lower extreme opening of outlet duct accesss to in the sampling pipe and be located in the sampling pipe on the liquid level of sampling liquid.

7. The automated sampling device of claim 6, wherein: and a rubber pad is arranged at the top end of the sampling tube and/or the bottom end of the cover body.