CN109486673B - Probiotics culture apparatus and culture dish - Google Patents
Probiotics culture apparatus and culture dish Download PDFInfo
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- CN109486673B CN109486673B CN201811478504.9A CN201811478504A CN109486673B CN 109486673 B CN109486673 B CN 109486673B CN 201811478504 A CN201811478504 A CN 201811478504A CN 109486673 B CN109486673 B CN 109486673B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention relates to a probiotic culture device and a culture plate, wherein the probiotic culture device comprises a main body, an inner gear ring is arranged on the periphery of the upper end surface of the main body, a central gear disc is arranged in the center of the upper end surface of the main body, a plurality of planetary gear discs are arranged around the central gear disc, the inner sides of the planetary gear discs are in meshing transmission with the central gear disc, the outer sides of the planetary gear discs are in meshing transmission with the inner gear ring at the upper end of the main body, a through hole is formed in the center of each planetary gear disc, a plurality of stacked culture plates are arranged at the upper end of each planetary gear disc, two guide holes are formed in the upper end surface of the main body, a lifting rod A and a lifting rod B are respectively inserted into the guide holes, the lifting rod A is driven by an electric push rod A to move up and down, a lifting rod motor is arranged between the lifting rod A and the electric push rod A, the lifting rod B is driven by the electric push rod B to move up and down, an electromagnet is arranged on the lifting rod A, and a flow channel and a nozzle are arranged in the lifting rod B. Simultaneously, the working method of the probiotic culture device and the culture tray thereof are also disclosed.
Description
Technical Field
The invention relates to the field of biological strain culture, in particular to a probiotic culture device and a culture tray.
Background
The probiotics are also called as prebiotics and prebiotics. It is currently widely believed that the mechanism of action of probiotics is to control the balance of microbial communities in the host, proliferate beneficial bacteria, and inhibit harmful bacteria, so that the beneficial bacteria become dominant flora, and thus benefit the host.
In the probiotic culture process, nutrient solution needs to be input into the probiotic culture device, so that the probiotic culture device can perform better fermentation culture, when the nutrient solution is input, the traditional method is only to arrange a spray head of the nutrient solution at the top of a culture vessel, the nutrient solution is sprayed at the upper end, but strains in the culture vessel are accumulated, the nutrient solution only falls into the strains on the top layer when being sprayed, the strains cannot deeply enter the inner strains, and therefore the utilization rate of the nutrient solution is not high.
Disclosure of Invention
In order to solve the technical problems in the background art, the invention provides a probiotic culture device and a culture tray, which have the advantages of higher probiotic yield, time and labor conservation, higher culture efficiency, real-time monitoring of environmental parameters in the probiotic culture process and more accurate control of the culture of probiotics.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the utility model provides a living fungus culture apparatus of benefit, including the main part, the built-in controlling means that is equipped with of main part, controlling means is PLC programmable logic controller, and the main part is cylindrical cavity, and the ring gear has been seted up to the periphery of the up end of main part, and central fluted disc has been installed at the center of main part up end, and central fluted disc is driven by central motor, central motor and controlling means electric connection, and central motor is the servo motor who takes the encoder.
The periphery of the central fluted disc is provided with a plurality of planetary fluted discs, the inner sides of the planetary fluted discs are in meshing transmission with the central fluted disc, the outer sides of the planetary fluted discs are in meshing transmission with the inner gear ring at the upper end of the main body, the lower end surfaces of the planetary fluted discs are provided with balls, and the planetary fluted discs realize rolling friction with the upper end surface of the main body through the balls. The center of the planetary fluted disc is provided with a through hole, the upper end of the planetary fluted disc is provided with a plurality of stacked culture discs, and the stacked culture discs are connected by a buckle.
The culture tray is made of transparent glass and comprises an outer barrel and an inner barrel which are arranged concentrically, the outer barrel and the inner barrel are fixedly connected through a bottom plate at the bottom, and the upper end face of the bottom plate is provided with a plurality of raised lines uniformly distributed on the circumference. The height of urceolus is greater than the height of inner tube, and the snap ring has been seted up to the upper end of urceolus, and the lower snap ring of lower with last snap ring assorted is seted up to the lower extreme of urceolus.
A plurality of circular shape archs have been seted up to the inboard of snap ring down, go up the inboard of snap ring and seted up a plurality ofly and protruding assorted draw-in groove, the draw-in groove is L shape recess, the long limit of L shape recess sets up along the circumference of cultivateing the dish, the minor face of L shape recess sets up along the axial of cultivateing the dish, can stack through last snap ring and lower snap ring between the cultivation dish, can carry out the buckle through draw-in groove and arch between the cultivation dish and connect.
The outer ring of the inner cylinder is provided with a ring groove, the ring groove is sleeved with a stirring ring, the stirring ring comprises a lantern ring, the outer ring of the lantern ring is provided with a plurality of stirring blades which are uniformly distributed on the circumference, the stirring ring is rotatably connected with the inner cylinder through the lantern ring, and the inner ring of the lantern ring is embedded with a permanent magnet.
Two guiding holes are formed in the upper end face of the main body, the guiding holes are equal to the distance of the central axis of the main body, a lifting rod A and a lifting rod B are respectively inserted into the guiding holes, the guiding holes are in sliding connection with the lifting rod A and the lifting rod B, and when the planetary fluted disc rotates to the position of the guiding holes, the lifting rod A or the lifting rod B can go deep into the culture disc through the guiding holes through the through holes in the centers of the planetary fluted discs. The lifting rod A is driven by the electric push rod A to move up and down, the electric push rod A is electrically connected with the control device, a lifting rod motor is arranged between the lifting rod A and the electric push rod A, the lifting rod motor can drive the lifting rod A to rotate, the electric push rod A is fixedly connected with the lifting rod motor through a fixing piece, an electromagnet is arranged on the lifting rod A, and the electromagnet can be matched with a permanent magnet on the culture disc in a magnetic attraction manner. The lifting rod B is driven by the electric push rod B to move up and down, the electric push rod B is electrically connected with the control device, a flow passage and a nozzle are formed in the lifting rod B, and liquid in the flow passage can be sprayed out through the nozzle.
The main part is built-in to be equipped with two containers, is equipped with heating rod and temperature sensor in the container, heating rod and temperature sensor and controlling means electric connection. The container passes through interface and external intercommunication, and the interface facial make-up is equipped with the valve, and the container has two, is used for holding culture solution and probiotic bacterial respectively, and the container facial make-up is equipped with the pump, and during the runner in the lifter B can be carried into through the hose to the liquid in the container to the pump, pump and controlling means electric connection.
The inside cavity of main part is equipped with a plurality of magnetic switch in, magnetic switch and controlling means electric connection, all install on mounting and the lifter B with magnetic switch assorted detection piece, can detect the high position of lifter A and lifter B through detecting piece and magnetic switch, distance between the adjacent magnetic switch equals with the thickness of cultivateing the dish.
The glass cover has been installed on the upper portion of main part, has installed detection device on the glass cover, and detection device contains temperature sensor, humidity transducer and carbon dioxide sensor in, and temperature sensor, humidity transducer and carbon dioxide sensor and controlling means electric connection. The glass cover is provided with an air filter which is electrically connected with the control device. The glass cover is provided with a lamp which is electrically connected with the control device.
A working method of a probiotic culture device comprises the following steps:
The central motor rotates, and the culture disc is rotated to the corresponding position of the lifting rod B under the driving of a gear transmission mechanism formed by the central fluted disc and the planetary fluted disc.
Electric putter B jacks up lifter B, makes lifter B's nozzle aim at every layer of cultivation dish in proper order to in the cultivation dish of every layer with bacterial and culture solution spraying, at this moment, correspond through the magnetic switch phase-match that detects piece and different positions department, can fix a position the high position that electric putter B stretched out. After spraying, the electric push rod B withdraws the lifting rod B into the main body.
The central motor rotates, the culture disc positioned at the corresponding position of the lifting rod B rotates to the corresponding position of the lifting rod A, the central motor is a servo motor with an encoder, the central motor can rotate at any angle, and the rotation angle of the central motor can realize accurate closed-loop control through the control device.
Electric putter A jacks up lifter A, and the electro-magnet on the lifter A rises to the position department corresponding with the permanent magnet on the culture dish, and the electro-magnet circular telegram, lifter motor drive lifter A rotate, and lifter A rotates through the magnetic force effect drive agitator ring between electro-magnet and the permanent magnet to mix the stirring to the benefit fungus of benefit and culture solution in the culture dish. After the stirring is completed, the electromagnet is powered off, and the electric push rod A withdraws the lifting rod A into the main body.
The center motor keeps periodic intermittent type to rotate, cultivates the dish and can carry out periodic rotation and revolution under the drive of center motor, at the rotation in-process of cultivateing the dish, cultivates the dish and can carry out lasting mixing stirring to the probiotic bacterial species wherein through the sand grip of bottom, effectively prevents separation and settlement between probiotic bacterial species and the culture solution.
And detect temperature, humidity and the carbon dioxide concentration of cultivateing the dish surrounding environment through detection device in real time, throw light on through the lamp, carry into the glass cover with external air through air cleaner, keep probiotic in a suitable cultivation environment.
The invention has the beneficial effects that:
(1) be provided with the cultivation dish that many places stacked, can cultivate simultaneously a plurality of cultivation dishes, the probiotic output is bigger.
(2) The automatic planting of bacterial, stirring mix and fermentation culture, labour saving and time saving, it is higher to cultivate efficiency.
(3) Environmental parameters in the process of probiotic culture are monitored in real time through various sensors, and the culture of probiotics can be controlled more accurately.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural diagram of a probiotic culture device according to the present invention;
FIG. 2 is a sectional view of section A of FIG. 1;
fig. 3 is a partially enlarged view at a position B of fig. 1;
FIG. 4 is an enlarged view of a portion of FIG. 1 at position C;
FIG. 5 is a schematic view of the structure of a culture dish of the present invention.
In the figure:
1. the automatic detection device comprises a culture disc, 2 a main body, 3 lifting rods A, 4 electromagnets, 5 lifting rod motors, 6 fixing pieces, 7 electric push rods A, 8 detection blocks, 9 magnetic switches, 10 control devices, 11 central motors, 12 central gear discs, 13 electric push rods B, 14 connectors, 15 containers, 16 pumps, 17 hoses, 18 lifting rods B, 19 planetary gear discs, 20 balls, 21 detection devices, 22 glass covers, 23 lamps and 24 air filters
101. The stirring device comprises an upper clamping ring, 102 clamping grooves, 103 outer cylinders, 104 lower clamping rings, 105 protrusions, 106 bottom plates, 107 inner cylinders, 108 stirring rings, 1081 permanent magnets, 1082 lantern rings, 1083 stirring sheets, 109 raised strips 181 flow channels and 182 nozzles.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The utility model provides a probiotic culture apparatus, including main part 2, the built-in controlling means 10 that is equipped with of main part 2, controlling means 10 is PLC programmable logic controller, main part 2 is cylindrical cavity, the ring gear has been seted up to the periphery of the up end of main part 2, central fluted disc 12 has been installed at the center of the 2 up end of main part, central fluted disc 12 is driven by central motor 11, central motor 11 and controlling means 10 electric connection, central motor 11 is the servo motor who takes the encoder.
The periphery of the central fluted disc 12 is provided with a plurality of planetary fluted discs 19, the inner sides of the planetary fluted discs 19 are in meshing transmission with the central fluted disc 12, the outer sides of the planetary fluted discs 19 are in meshing transmission with the inner gear ring at the upper end of the main body 2, the lower end surfaces of the planetary fluted discs 19 are provided with balls 20, and the planetary fluted discs 19 realize rolling friction with the upper end surface of the main body 2 through the balls 20. The center of the planetary fluted disc 19 is provided with a through hole, the upper end of the planetary fluted disc 19 is provided with a plurality of stacked culture discs 1, and the stacked culture discs 1 are connected in a buckling way.
The culture tray 1 is made of transparent glass, the culture tray 1 comprises an outer barrel 103 and an inner barrel 107 which are concentrically arranged, the outer barrel 103 and the inner barrel 107 are fixedly connected through a bottom plate 106 at the bottom, the height of the outer barrel 103 is larger than that of the inner barrel 107, an upper snap ring 101 is arranged at the upper end of the outer barrel 103, and a lower snap ring 104 matched with the upper snap ring 101 is arranged at the lower end of the outer barrel 103.
A plurality of circular shape protruding 105 have been seted up to the inboard of lower snap ring 104, go up the inboard of snap ring 101 and seted up a plurality ofly and protruding 105 assorted draw-in grooves 102, draw-in groove 102 is L shape recess, the long limit of L shape recess sets up along the circumference of cultivateing set 1, the minor face of L shape recess sets up along the axial of cultivateing set 1, can stack through last snap ring 101 and lower snap ring 104 between the cultivateing set 1, can carry out the buckle through draw-in groove 102 and protruding 105 between the cultivateing set 1 and connect.
An annular groove is formed in the outer ring of the inner cylinder 107, a stirring ring 108 is sleeved on the annular groove, the stirring ring 108 comprises a lantern ring 1082, a plurality of stirring blades 1083 which are uniformly distributed on the circumference are arranged on the outer ring of the lantern ring 1082, the stirring ring 108 is rotatably connected with the inner cylinder 107 through the lantern ring 1082, and a permanent magnet 1081 is embedded in the inner ring of the lantern ring 1082.
The upper end surface of the bottom plate 106 is provided with a plurality of raised strips 109 which are evenly distributed on the circumference.
Two guiding holes are formed in the upper end face of the main body 2, the guiding holes are equal to the central axis of the main body 2 in distance, a lifting rod A3 and a lifting rod B18 are respectively inserted into the guiding holes, the guiding holes are in sliding connection with the lifting rod A3 and the lifting rod B18, and when the planetary fluted disc 19 rotates to the position of the guiding holes, the lifting rod A3 or the lifting rod B18 can penetrate into the culture disc 1 through the guiding holes through the through holes in the center of the planetary fluted disc 19. Lifter A3 is by its up-and-down motion of electric putter A7 drive, and electric putter A7 and controlling means 10 electric connection have installed lifter motor 5 between lifter A3 and the electric putter A7, and lifter motor 5 can drive lifter A3 and rotate, through 6 fixed connection of mounting between electric putter A7 and the lifter motor 5. The lifting rod A3 is provided with an electromagnet 4, and the electromagnet 4 can be matched with a permanent magnet 1081 on the culture disc 1 in a magnetic attraction manner. The lifting rod B18 is driven by the electric push rod B13 to move up and down, the electric push rod B13 is electrically connected with the control device 10, a flow passage 181 and a nozzle 182 are formed in the lifting rod B18, and liquid in the flow passage 181 can be sprayed out through the nozzle 182.
Two containers 15 are arranged in the main body 2, and a heating rod and a temperature sensor are arranged in the containers 15 and are electrically connected with the control device 10. The container 15 is communicated with the outside through the interface 14, a valve is arranged on the interface 14, the container 15 is provided with two positions for containing culture solution and probiotic strains respectively, a pump 16 is arranged on the container 15, the pump 16 can convey the liquid in the container 15 into a flow passage 181 in the lifting rod B18 through a hose 17, and the pump 16 is electrically connected with the control device 10.
The inside cavity of main part 2 is equipped with a plurality of magnetic switch 9 in, and magnetic switch 9 and controlling means 10 electric connection, all install on mounting 6 and lifter B18 with magnetic switch 9 assorted detection piece 8, can detect the high position of lifter A3 and lifter B18 through detecting piece 8 and magnetic switch 9, the distance between the adjacent magnetic switch 9 equals with the thickness of cultivateing dish 1.
The upper portion of main part 2 has been installed glass cover 22, and glass cover 22 facial make-up is equipped with detection device 21, contains temperature sensor, humidity transducer and carbon dioxide sensor in the detection device 21, and temperature sensor, humidity transducer and carbon dioxide sensor and controlling means 10 electric connection. The glass cover 22 is provided with an air filter, and the air filter is electrically connected with the control device 10. The glass cover 22 is provided with a lamp 23, and the lamp 23 is electrically connected with the control device 10.
The specific working method comprises the following steps:
The central motor 11 rotates, and the culture disc 1 is rotated to the corresponding position of the lifting rod B18 under the driving of a gear transmission mechanism formed by the central fluted disc 12 and the planet fluted disc 19.
The electric push rod B13 jacks up the lifting rod B18, so that the nozzle 182 of the lifting rod B18 is sequentially aligned with the culture tray 1 on each layer, strains and culture solution are sprayed into the culture tray 1 on each layer, and at the moment, the detection block 8 is matched and corresponding to the magnetic switches 9 on different positions, so that the extending height position of the electric push rod B13 can be positioned. After spraying, the electric push rod B13 retracts the lifting rod B18 into the main body 2.
The central motor 11 rotates to rotate the culture tray 1 located at the corresponding position of the lifting rod B18 to the corresponding position of the lifting rod A3, and the central motor 11 is a servo motor with an encoder and can rotate at any angle, and the rotation angle of the central motor can be accurately controlled in a closed loop mode through the control device 10.
Electric putter A7 jacks up lifter A3, and 4 electro-magnets on lifter A3 rise to with cultivate the corresponding position department of permanent magnet 1081 on the dish 1, and electro-magnet 4 circular telegram, lifter motor 5 drive lifter A3 rotates, lifter A3 through the magnetic force effect drive stirring ring 108 between electro-magnet 4 and the permanent magnet 1081 and rotate to the mixed stirring of probiotic bacterial and culture solution in the dish 1 that cultivates. After the stirring is finished, the electromagnet 4 is powered off, and the electric push rod A7 retracts the lifting rod A3 into the main body 2.
The central motor 11 keeps periodic intermittent rotation, the culture tray 1 can rotate and revolve periodically under the driving of the central motor 11, and in the rotation process of the culture tray 1, the culture tray 1 can continuously mix and stir probiotics in the culture tray through the raised lines 109 at the bottom, so that the separation and sedimentation between the probiotics and the culture solution are effectively prevented.
And the temperature, humidity and carbon dioxide concentration of the surrounding environment of the culture tray 1 are detected by the detection device 21 in real time, the lamp 23 is used for lighting, and the outside air is conveyed into the glass cover 22 through the air filter 24 to keep the probiotics in a proper culture environment.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (7)
1. A probiotic culture device which characterized in that:
comprises a main body (2), a control device (10) is arranged in the main body (2),
the main body (2) is a cylindrical cavity, an inner gear ring is arranged on the periphery of the upper end surface of the main body (2), a central fluted disc (12) is arranged at the center of the upper end surface of the main body (2), the central fluted disc (12) is driven by a central motor (11), the central motor (11) is electrically connected with a control device (10),
a plurality of planet fluted discs (19) are arranged around the central fluted disc (12), the inner sides of the planet fluted discs (19) are in meshing transmission with the central fluted disc (12), the outer sides of the planet fluted discs (19) are in meshing transmission with the inner gear ring at the upper end of the main body (2),
the lower end surface of the planet fluted disc (19) is provided with balls (20), the planet fluted disc (19) realizes rolling friction with the upper end surface of the main body (2) through the balls (20),
a through hole is arranged at the center of the planetary fluted disc (19), a plurality of stacked culture discs (1) are arranged at the upper end of the planetary fluted disc (19), the stacked culture discs (1) are connected in a buckling way,
the culture tray (1) comprises an outer cylinder (103) and an inner cylinder (107) which are concentrically arranged, the outer cylinder (103) and the inner cylinder (107) are fixedly connected through a bottom plate (106) at the bottom, the height of the outer cylinder (103) is greater than that of the inner cylinder (107),
the upper end of the outer cylinder (103) is provided with an upper snap ring (101), the lower end of the outer cylinder (103) is provided with a lower snap ring (104) matched with the upper snap ring (101), the culture trays (1) can be stacked through the upper snap ring (101) and the lower snap ring (104),
the inner side of the lower snap ring (104) is provided with a plurality of bulges (105), the inner side of the upper snap ring (101) is provided with a plurality of clamping grooves (102) matched with the bulges (105), the culture trays (1) can be connected by the clamping grooves (102) and the bulges (105) in a buckling way,
an annular groove is formed in the outer ring of the inner cylinder (107), a stirring ring (108) is sleeved on the annular groove, the stirring ring (108) comprises a lantern ring (1082), a plurality of stirring blades (1083) which are uniformly distributed on the circumference are arranged on the outer ring of the lantern ring (1082), the stirring ring (108) is rotatably connected with the inner cylinder (107) through the lantern ring (1082),
the inner ring of the lantern ring (1082) is embedded with a permanent magnet (1081),
two guide holes are formed in the upper end face of the main body (2), the guide holes are equal to the central axis of the main body (2) in distance, a lifting rod A (3) and a lifting rod B (18) are respectively inserted into the guide holes, the guide holes are in sliding connection with the lifting rod A (3) and the lifting rod B (18), when the planetary fluted disc (19) rotates to the positions of the guide holes, the lifting rod A (3) or the lifting rod B (18) can penetrate into the culture disc (1) through the guide holes through a through hole in the center of the planetary fluted disc (19),
the lifting rod A (3) is driven to move up and down by an electric push rod A (7), the electric push rod A (7) is electrically connected with a control device (10), a lifting rod motor (5) is arranged between the lifting rod A (3) and the electric push rod A (7), the lifting rod motor (5) can drive the lifting rod A (3) to rotate, the electric push rod A (7) is fixedly connected with the lifting rod motor (5) through a fixing piece (6), an electromagnet (4) is arranged on the lifting rod A (3), the electromagnet (4) can be matched with a permanent magnet (1081) on the culture disc (1) in a magnetic attraction way,
the lifting rod B (18) is driven by an electric push rod B (13) to move up and down, the electric push rod B (13) is electrically connected with the control device (10), a flow passage (181) and a nozzle (182) are formed in the lifting rod B (18), liquid in the flow passage (181) can be sprayed out through the nozzle (182),
the main body (2) is internally provided with two containers (15), the containers (15) are communicated with the outside through an interface (14), the interface (14) is provided with a valve, the containers (15) are provided with two parts which are respectively used for containing culture solution and probiotic strains, the containers (15) are provided with a pump (16), the pump (16) can convey the liquid in the containers (15) into a flow passage (181) in the lifting rod B (18) through a hose (17), and the pump (16) is electrically connected with the control device (10),
a plurality of magnetic switches (9) are arranged in an inner cavity of the main body (2), the magnetic switches (9) are electrically connected with the control device (10), the fixing piece (6) and the lifting rod B (18) are provided with detection blocks (8) matched with the magnetic switches (9), the height positions of the lifting rod A (3) and the lifting rod B (18) can be detected through the detection blocks (8) and the magnetic switches (9), the distance between every two adjacent magnetic switches (9) is equal to the thickness of the culture tray (1),
the upper part of the main body (2) is provided with a glass cover (22), the glass cover (22) is provided with a detection device (21), the detection device (21) comprises a temperature sensor, a humidity sensor and a carbon dioxide sensor, the temperature sensor, the humidity sensor and the carbon dioxide sensor are electrically connected with the control device (10),
the glass cover (22) is provided with an air filter, and the air filter is electrically connected with the control device (10).
2. A probiotic culture device according to claim 1, characterized in that:
the glass cover (22) is provided with a lamp (23), and the lamp (23) is electrically connected with the control device (10).
3. A probiotic culture device according to claim 1, characterized in that:
the central motor (11) is a servo motor with an encoder.
4. A probiotic culture device according to claim 1, characterized in that:
and a heating rod and a temperature sensor are arranged in the container (15), and the heating rod and the temperature sensor are electrically connected with the control device (10).
5. A culture dish for a probiotic culture device, characterized in that:
the culture tray (1) comprises an outer cylinder (103) and an inner cylinder (107) which are concentrically arranged, the outer cylinder (103) and the inner cylinder (107) are fixedly connected through a bottom plate (106) at the bottom, the height of the outer cylinder (103) is greater than that of the inner cylinder (107),
the upper end of the outer cylinder (103) is provided with an upper snap ring (101), the lower end of the outer cylinder (103) is provided with a lower snap ring (104) matched with the upper snap ring (101), the culture trays (1) can be stacked through the upper snap ring (101) and the lower snap ring (104),
the inner side of the lower snap ring (104) is provided with a plurality of bulges (105), the inner side of the upper snap ring (101) is provided with a plurality of clamping grooves (102) matched with the bulges (105), the culture trays (1) can be connected by the clamping grooves (102) and the bulges (105) in a buckling way,
an annular groove is formed in the outer ring of the inner cylinder (107), a stirring ring (108) is sleeved on the annular groove, the stirring ring (108) comprises a lantern ring (1082), a plurality of stirring blades (1083) which are uniformly distributed on the circumference are arranged on the outer ring of the lantern ring (1082), the stirring ring (108) is rotatably connected with the inner cylinder (107) through the lantern ring (1082),
the inner ring of the lantern ring (1082) is embedded with a permanent magnet (1081),
the upper end face of the bottom plate (106) is provided with a plurality of raised lines (109) which are uniformly distributed on the circumference.
6. A culture tray for a probiotic culture device, according to claim 5, characterized in that:
the protrusion (105) is a circular protrusion, the clamping groove (102) is an L-shaped groove, the long edge of the L-shaped groove is arranged along the circumferential direction of the culture disc (1), and the short edge of the L-shaped groove is arranged along the axial direction of the culture disc (1).
7. A culture tray for a probiotic culture device, according to claim 5, characterized in that:
the culture dish (1) is made of transparent glass.
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| CN109486673B true CN109486673B (en) | 2022-03-01 |
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| CN111004711A (en) * | 2020-02-14 | 2020-04-14 | 广西贵港华堂天诺微生物科技有限公司 | Microbial cultivation jar device |
| CN113621485B (en) * | 2021-08-31 | 2023-06-09 | 宁夏职业技术学院（宁夏广播电视大学） | A device for morchella liquid strain fermentation |
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