CN104818214A - Intelligent culture dish - Google Patents

Intelligent culture dish Download PDF

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Publication number
CN104818214A
CN104818214A CN201510197873.0A CN201510197873A CN104818214A CN 104818214 A CN104818214 A CN 104818214A CN 201510197873 A CN201510197873 A CN 201510197873A CN 104818214 A CN104818214 A CN 104818214A
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China
Prior art keywords
driving
layer
culture dish
deformable substrate
intelligent culture
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CN201510197873.0A
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Chinese (zh)
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CN104818214B (en
Inventor
李铁风
李驰
谢雨涵
杨栩旭
刘俊杰
黄晓强
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Zhejiang University ZJU
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Zhejiang University ZJU
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Priority to CN201510197873.0A priority Critical patent/CN104818214B/en
Publication of CN104818214A publication Critical patent/CN104818214A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting

Abstract

The invention discloses an intelligent culture dish. The intelligent culture dish comprises a dish body, a drive circuit and a control module, an electrically driven deformable substrate is arranged at the bottom in the dish body, and the drive circuit provides corresponding drive voltage for the deformable substrate under the control of the control module to drive the deformable substrate to deform correspondingly. The intelligent culture dish uses the electrically driven deformable substrate to carry out vibrating stirring on culture substance, and directed stirring, bending deformation, rotational stirring or the like is carried out on the culture substance according to the need. By means of the electric control, the structure is simple, the manufacturing is easy to perform, and the cost is low; the intelligent culture dish can be disposable, and the intelligent culture dish can provide different stirring functions.

Description

A kind of Intelligent culture ware
Technical field
The present invention relates to microorganism and technical field of cell culture, be specifically related to a kind of Intelligent culture ware.
Background technology
Culture dish is a kind of labware for microorganism or cell cultures, is made up of, generally makes with glass or plastics the end of a flat circle plate-like and a lid.
Traditional culture dish mostly is simple glass or plastic ware, mainly in field of biology for cell cultures, its do not have vibration and agitating function.
In recent years for meeting various cultivation needs, there is novel damping culture dish, the utility application being 202671544U as publication number discloses a kind of damping culture dish, comprise ware body and ware lid, the bottom of ware body is provided with buffer layer, be provided with glue layer between buffer layer and ware body, buffer layer is foam layer.This damping culture dish when operations such as culturing cells, can avoid culture dish high vibration in handling process, reduces vibration and causes disadvantageous effect to the growth of culture in culture dish.
On this basis, publication number is the Novel culture dish that the utility application of 203048939U discloses a kind of sealed vibration proof, belong to Experiment on Microbiology vessel, comprise culture dish body, and the gland be located at above culture dish body, gland inwall is provided with draw-in groove, the bottom of culture dish body is provided with buffer layer, and buffer layer upper end is provided with thermal insulation layer, thermal insulation layer is provided with cultivation region, culture dish body outer wall is provided with the boss matched with draw-in groove on lid, and culture dish body lower end is provided with base.Draw-in groove and boss can make culture dish body and gland mutually clamp, and become a substantially airtight space.Buffer layer can avoid culture dish in handling process, have violent vibration sense, reduces vibration and causes disadvantageous effect to the growth of culture in culture dish.
And for Bioexperiment, except the needs of damping, for some special applications, need to create vibration, Vibratory Mixing is carried out to the nutrient solution in culture dish.Because the culture materials such as cell are general more fragile, easily damage, traditional alr mode (as stirring rod) therefore can not be used to stir, can only stir by other means.
The existing culture dish that vibrates realizes vibration by ultrasonic vibration mostly, although it can realize the vibration to culture material in culture dish, but can not control direction and the position of vibration, ultrasonic vibration technology also has cost higher, uses price shortcoming costly simultaneously.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of Intelligent culture ware, the control of direction of vibration and position can be realized, and with low cost.
A kind of Intelligent culture ware, comprise inner bottom part and be provided with the ware body of electrically driven (operated) deformable substrate, driving circuit and control module, for deformable substrate provides corresponding driving voltage, corresponding deformation is there is to drive deformable substrate in described driving circuit under the control of control module.
Culture dish of the present invention produces vibration by the deformation of the electric drive deformable substrate of ware body inner bottom part.Because this deformable substrate is electric drive deformable substrate, therefore, can, by the control of electric control realization to culture dish vibration modes, be easy to realize.
In the present invention, ware body can adopt existing glass culture dish etc.
Described deformable substrate comprises protective layer from top to bottom successively, and upper and lower surface is provided with the driving layer of flexible electrode layer, is embedded with rigidity and is greater than the functional fibre driving film in described driving layer;
The flexible electrode layer on the upper and lower surface of described driving layer cooperatively interacts the driving voltage receiving driving circuit output.
In driving layer, embedding rigidity, to be greater than the object of functional fibre driving film be to limit driving layer deformation direction, and then reach and realize culture dish vibration in particular directions.For ensureing the tolerance range of direction of vibration, functional fibre is the bigger the better with driving the stiffness difference of film.As preferably, the rigidity of described functional fibre is the rigidity more than 10 times driving film.
When the flexible electrode on the upper and lower surface of driving layer applying respectively positive and negative voltage, film homonymy homocharge repels mutually makes membrane surface area become large, film heteropleural heterocharge attracts each other and makes film thickness thinning, the result of both comprehensive actions makes film produce area change greatly exactly, the distortion of lower thickness, when removing voltage, film returns to the original form.
As preferably, described driving layer is dielectric elastomerics film, and thickness is 0.2 ~ 2mm.Further preferably, flexible electrode layer adopts carbon paste, the materials such as electric silica gel.
In addition, due to flexible electrode layer conduction, outside exposed, easily produce electric leakage accident.Protective layer insulation in the present invention and be flexible, the protective layer is namely flexible insulation protective layer.
Flexible electrode layer and protective layer is blocked up can affect Oscillation Amplitude, therefore, as preferably, the thickness of described flexible electrode layer is 0.05 ~ 0.3mm, and is less than the thickness for described driving layer.The thickness of described protective layer is 0.1 ~ 0.5mm.
For assurance function fiber can embed in driving layer, the diameter of described functional fibre is 0.1 ~ 0.5mm, and is less than the thickness of driving layer.
The amplitude of culture dish depends in described deformable substrate the material and thickness that respectively form layer (comprising driving layer, flexible electrode layer and protective layer); and the material of functional fibre and diameter; in field of cell culture; because cell itself is more fragile; easy damage, therefore has certain restriction for Oscillation Amplitude, and amplitude should be less than 3mm usually; as preferably, amplitude is 0.5mm ~ 2mm.Accordingly, under these conditions, the driving voltage that described driving circuit provides is 2kV ~ 20kV, and under control module effect within the scope of this continuously adjustabe.
In addition, insulate between the flexible electrode for the guarantee upper and lower surface of driving layer, the shortest distance at the flexible electrode layer Edge Distance driving layer edge on the upper and lower surface of described driving layer is 5 ~ 10mm, and optimum is 5mm.
Compared with prior art, Intelligent culture ware of the present invention utilizes the substrate of DE composite transformable to carry out Vibratory Mixing to culture material, carries out orientation as required and stirs, flexural deformation, Stirring etc. to culture material.And by electric control, structure is simple, make easily, cost is very low, can single use, can provide multiple agitating function simultaneously.
Accompanying drawing explanation
Fig. 1 is the structural representation of the Intelligent culture ware of the present embodiment;
Fig. 2 is the structural representation of deformable substrate;
Fig. 3 functional fibre linearly type arrangement schematic diagram;
Fig. 4 functional fibre is the schematic diagram of square matrix-shaped arrangement;
Fig. 5 functional fibre is the schematic diagram of scroll arrangement.
Embodiment
Describe the present invention below in conjunction with the drawings and specific embodiments.
A kind of Intelligent culture ware, as shown in Figure 1, comprise inner bottom part and be provided with the ware body 2 of electric drive deformable substrate 1, driving circuit 3 and control module 4, for deformable substrate provides corresponding driving voltage, corresponding deformation is there is to drive deformable substrate 1 in driving circuit 3 under the control of control module 4.
As shown in Figure 2, deformable substrate comprises protective layer 5 from top to bottom successively, and upper and lower surface is provided with the driving layer 6 of flexible electrode layer 8, is embedded with the functional fibre 7 that rigidity is greater than driving layer 6 in driving layer 6.In the present embodiment deformable substrate, the flexible electrode layer 8 of driving layer 6 lower surface is also provided with protective layer 5.
During practical application, controlled the size of the driving voltage that driving circuit 3 provides by control module 4, make driving layer 6 that corresponding deformation occur.And in driving layer 6, being embedded with the functional fibre 7 that rigidity is greater than driving layer 6, the deformation in the place having functional fibre 7 to embed is restricted, and then makes culture dish form specific vibration modes.
In the present embodiment, driving layer 6 is dielectric elastomerics film, is specially 3M-VHB 4910 material, and thickness is 2mm.
Be located thereon, the flexible electrode layer 8 of lower surface can adopt carbon paste, the materials such as electric silica gel.Be carbon paste in the present embodiment, thickness is 0.1mm.For ensure upper and lower surface flexible electrode layer between electric isolution, the minor increment at the edge of the Edge Distance driving layer of the flexible electrode layer on upper and lower surface is 5mm.
The effect of protective layer 5 is that flexible electrode layer is encapsulated in inside, avoids it to contact with extraneous; In the present embodiment protective layer insulation and be flexibility, prepared by 3M-VHB 9473 material, thickness is 0.1mm.
For assurance function fiber 7 can be embedded in driving layer 6, the diameter of functional fibre 7 is less than the thickness of driving layer 6, and in the present embodiment, the diameter of functional fibre is 0.3mm.
The rigidity of functional fibre is larger relative to driving layer stiffness difference, and restriction is stronger.Usually require that the rigidity of functional fibre is the rigidity more than 10 times driving film.Nylon, plastics, rubber etc. rigidity can be adopted to be that the insulating material material of driving layer rigidity more than 10 times prepares, and as nylon wire, plastic wire, rubber thread is nylon wire in the present embodiment.
The arrangement mode of functional fibre in driving layer is different, and the type of drive of the culture dish that correspondence obtains is different.
A. as shown in Figure 3, when linearly type arranges functional fibre, namely each root functionality fiber arranges according to the form of equidistant line, and be embedded in the middle of driving film, the part wherein scribbling flexible electrode is for can drive part, be called active membrane, the part not being coated with the driving film of flexible electrode for can not drive part, be called passive film).Because fiber limits active membrane distortion in the horizontal direction, therefore this film can only be out of shape at vertical direction, and deflection is than large when not adding fiber.
When energized, film substrate active part is elongated at vertical direction, and during power-off, this part is shunk again, and linear pattern fiber can realize culture dish to the tension and compression of culture material at specific direction as can be seen here.
B. as shown in Figure 4, when functional fibre is square matrix-shaped arrangement, namely functional fibre is divided into horizontal and vertical arrangement and forms several closed regions, is randomly dispersed in driving layer, only in the closed region that functional fibre surrounds, scribble flexible electrode, and be not coated with flexible electrode outside this region.
During energising, active membrane in closed region will produce area and become large, the distortion of lower thickness, and functional fibre is owing to being dead front type, self can not produce this distortion, because which limit the distortion of active membrane in thin film planar, thus cause active membrane can only be out of shape perpendicular on the direction of thin film planar, and then form multiple raised points, the protrusion direction of this raised points is randomly dispersed in film both sides, again as shown in Figure 1, because the lower end of deformable substrate directly contacts with the culture dish shell of rigidity, therefore this raised points can only raise up and can not to lower convexity, can be realized the flexural deformation of culture material in culture dish at specific direction by the position of this kind of raised points of reasonable Arrangement.
C. as shown in Figure 5, functional fibre is scroll arrangement.After energising, active membrane outside thread area can Free Transform, and the active membrane in thread area is due to the restriction of helical, can only be out of shape along the back-out direction of helical, the active membrane in thread area therefore can be made to produce the rotational deformation of certain angle.In the spiral formation mode shown in Fig. 5, during energising, active membrane produces anticlockwise rotation, and power-off rear film produces the clockwise state before recovering distortion that rotates.Culture dish can be realized to the rotation of culture material and stirring action by this helical fiber type.
According to the deformation principle of electrically driven (operated) deformable substrate, in the present embodiment control module be mainly used in control driving circuit export driving voltage there is corresponding deformation to control driving layer.Therefore the size of driving voltage that driving circuit provides directly controls the amplitude of whole culture dish.
For requiring that the amplitude of culture dish is the culture dish of 0.5mm ~ 2mm in the present embodiment, therefore, the driving voltage that driving circuit provides is 2kV ~ 20kV, and driving voltage continuously adjustabe within the scope of this that driving circuit provides, concrete regulative mode is controlled the control of module.
Above-described embodiment has been described in detail technical scheme of the present invention and beneficial effect; be understood that and the foregoing is only most preferred embodiment of the present invention; be not limited to the present invention; all make in spirit of the present invention any amendment, supplement and equivalent to replace, all should be included within protection scope of the present invention.

Claims (8)

1. an Intelligent culture ware, it is characterized in that, comprise ware body, driving circuit and control module, the inner bottom part of described ware body is provided with electrically driven (operated) deformable substrate, for deformable substrate provides corresponding driving voltage, corresponding deformation is there is to drive deformable substrate in driving circuit under the control of control module.
2. Intelligent culture ware as claimed in claim 1, it is characterized in that, described deformable substrate comprises protective layer from top to bottom successively, and upper and lower surface is provided with the driving layer of flexible electrode layer, is embedded with the functional fibre that rigidity is greater than driving layer in described driving layer; The flexible electrode layer on the upper and lower surface of described driving layer cooperatively interacts the driving voltage receiving driving circuit output.
3. Intelligent culture ware as claimed in claim 2, is characterized in that, the rigidity of described functional fibre is the rigidity more than 10 times driving film.
4. Intelligent culture ware as claimed in claim 3, it is characterized in that, described driving layer is dielectric elastomerics film.
5. Intelligent culture ware as claimed in claim 4, it is characterized in that, the thickness of described driving layer is 0.2 ~ 2mm.
6. Intelligent culture ware as claimed in claim 4, it is characterized in that, the diameter of described functional fibre is 0.1 ~ 0.5mm, and is less than the thickness of driving layer.
7. Intelligent culture ware as claimed in claim 4, it is characterized in that, the thickness of described flexible electrode layer is 0.05 ~ 0.3mm, and is less than the thickness for described driving layer.
8. as the Intelligent culture ware in claim 4 ~ 7 as described in any one, it is characterized in that, the driving voltage that described driving circuit provides is 2kV ~ 20kV.
CN201510197873.0A 2015-04-23 2015-04-23 A kind of Intelligent culture ware Expired - Fee Related CN104818214B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105039159A (en) * 2015-08-25 2015-11-11 南京康擎新材料科技有限公司 Biological culture dish with multiple working modes
CN108070519A (en) * 2017-11-26 2018-05-25 东莞市松研智达工业设计有限公司 A kind of Tissue Culture Dish
CN108085243A (en) * 2017-11-26 2018-05-29 东莞市松研智达工业设计有限公司 Matrix form Tissue Culture Dish carrying case
CN109486673A (en) * 2018-12-05 2019-03-19 商庆尧 A kind of probiotic's culture device and working method and culture plate

Citations (2)

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Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN103688318A (en) * 2011-03-01 2014-03-26 拜耳知识产权有限责任公司 Automated manufacturing processes for producing deformable polymer devices and films
CN103764812A (en) * 2011-08-30 2014-04-30 生物梅里埃公司 Methods for inoculating culture media on petri dishes by means of vibration frequencies

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105039159A (en) * 2015-08-25 2015-11-11 南京康擎新材料科技有限公司 Biological culture dish with multiple working modes
CN108070519A (en) * 2017-11-26 2018-05-25 东莞市松研智达工业设计有限公司 A kind of Tissue Culture Dish
CN108085243A (en) * 2017-11-26 2018-05-29 东莞市松研智达工业设计有限公司 Matrix form Tissue Culture Dish carrying case
CN109486673A (en) * 2018-12-05 2019-03-19 商庆尧 A kind of probiotic's culture device and working method and culture plate

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