CN108485941B - Photosynthetic bacterium culture method - Google Patents
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Abstract
The invention belongs to the technical field of bacterial culture, in particular to a photosynthetic bacteria culture method, which adopts a photosynthetic bacteria culture instrument, wherein the culture instrument comprises a glass tank, a luminous plate, a power generation plate, an acid-base solution box, a motor, a rotating shaft, a stirring module, an acid-base regulation module, a rotating disc, a motor I, a feed inlet and a discharge outlet, and the glass tank is used for containing a culture medium solution and photosynthetic bacteria; the light-emitting plate is concentrically arranged on the outer side of the glass tank; the acid-base solution tank is positioned above the tank cover; the motor is positioned above the acid-base solvent box; the stirring module is used for stirring the photosynthetic bacteria; the acid-base adjusting module is arranged at the upper end of the rotating shaft and is positioned in the glass tank body; the rotating disc is positioned below the glass tank; the motor I and the rotating disc are fixedly installed; the feed inlet is positioned at the upper end of the glass tank; the discharge hole is positioned at the bottom of the glass tank. The invention is mainly used for culturing photosynthetic bacteria, can provide proper culture temperature and timely regulate the pH value of a culture solution.
Description
Technical Field
The invention belongs to the technical field of bacterial culture, and particularly relates to a photosynthetic bacteria culture method.
Background
Photosynthetic bacteria (PSB) are prokaryotes having an original light energy synthesis system, which are ubiquitous in nature and appear earliest on the earth, and are a general term for bacteria that perform anaerobic photosynthesis under anaerobic conditions, and they are microorganisms that perform photosynthesis using light as an energy source and using organic matter, sulfide, ammonia, and the like in nature as a hydrogen donor and carbon source under anaerobic illumination or aerobic dark conditions. Photosynthetic bacteria have been widely used in the fields of wastewater treatment, aquaculture, agricultural production increase, medicine and health care. Photosynthetic bacteria culture generally uses artificial light source for long-term irradiation as energy source, and the energy consumption is large. In addition, the phototactic adherence of the photosynthetic bacteria can cause the photosynthetic bacteria to be attached to the wall of the culture device, so that light is prevented from penetrating through the wall of the culture device to enter the culture device, the intensity of the light is reduced, the utilization efficiency of the light and the growth rate of the photosynthetic bacteria are influenced, and the current photosynthetic bacteria culture device cannot treat the adherent photosynthetic bacteria thoroughly and timely.
In view of this, the photosynthetic bacteria culture method of the present invention can provide a suitable culture temperature through the culture apparatus, and timely adjust the ph value of the culture solution.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a photosynthetic bacteria culture method which is mainly used for culturing photosynthetic bacteria, wherein a stirring module and an acid-base adjusting module in a culture instrument are matched with a rotating shaft to rotate, so that the photosynthetic bacteria are uniformly mixed on one hand, the light gathering and wall adhering on the other hand are effectively treated, the pH value of a culture solution is controlled, and a suitable production environment for the photosynthetic bacteria is provided.
The technical scheme adopted by the invention for solving the technical problems is as follows: the photosynthetic bacteria culture method adopts a photosynthetic bacteria culture instrument, wherein the culture instrument comprises a glass tank, a luminous plate, a power generation plate, an acid-base solution tank, a motor, a rotating shaft, a stirring module, an acid-base adjusting module, a rotating disc, a motor I, a feed inlet and a discharge outlet, the glass tank is cylindrical and is used for containing a culture medium solution and photosynthetic bacteria; the light-emitting plate is concentrically arranged on the outer side of the glass tank; the power generation plate is concentrically arranged on the outer side of the light emitting plate; the acid-base solution tank is positioned above the tank cover; the motor is positioned above the acid-base solvent box; the rotating shaft is arranged on the motor and penetrates through the acid-base solution tank; the stirring module is arranged on the rotating shaft and is used for stirring the photosynthetic bacteria; the acid-base adjusting module is arranged at the upper end of the rotating shaft and is positioned in the glass tank body; the rotating disc is positioned below the glass tank; the first motor and the rotating disc are fixedly installed; the feeding hole is positioned at the upper end of the glass tank; the discharge hole is positioned at the bottom of the glass tank;
the specific method comprises the following steps:
firstly, preparing photosynthetic bacteria culture solution according to the following preparation ratio: 500 g of medium: 80 liters of water: 20 liters of strains;
step two, after the step one is finished, adding the prepared photosynthetic bacteria culture solution into a glass jar through a feeding hole;
step three, after the step two is finished, illumination culture: the light-emitting plate is turned on to provide sufficient illumination energy, the convex lens arranged in the interlayer of the glass tank is used for condensing and heating, and the rotating disc drives the outer layer of the glass tank to rotate so as to uniformly heat the photosynthetic bacteria liquid;
step four, after the step three is finished, controlling the culture temperature to be 30-38 ℃;
after the fourth step is finished, the motor is started, the stirring rod drives the bacterium brush to rotate, the photosynthetic bacteria attached to the inner wall of the glass tank are brushed away, and the phenomenon that light condensation adheres to the wall and influences the effective growth of the photosynthetic bacteria inside is avoided; the detector controls the micro telescopic cylinder to work in time according to the acidity of the culture solution in the glass tank, pushes the piston to press out the calcium-containing solution in the stirring rod, and adjusts the cultured pH value;
and step six, after the step five is finished, the photosynthetic bacteria are poured out from the discharge hole after being cultured for 3-5 days.
Preferably, the glass jar be double-deck glass jar, be equipped with convex lens between the double glazing of glass jar, convex lens is used for the effect that spotlight heated, the outer glass of glass jar can rotate, glass jar inner wall evenly is equipped with the lug. During operation, the convex lens condenses light and heats the light-emitting plate, adjusts the temperature in the glass tank and promotes the fastest growth of photosynthetic bacteria.
Preferably, the rotating shaft is divided into two sections, the upper section is provided with a hollow chamber, the hollow chamber is communicated with the acid-base adjusting module and the acid-base solution tank, the lower section is of a solid structure, and the lower section is provided with a thread structure. The lower section of the rotating shaft is in threaded connection with the stirring module, the stirring module can move up and down along with the rotating shaft, and a hollow chamber formed by the upper section of the rotating shaft is communicated with the acid-base adjusting module, so that acid-base liquid in the acid-base adjusting module can be supplemented in time.
Preferably, the stirring module comprises a stirring rod, an electric heating rod and a first bacteria brush, and the stirring rod is arranged on the rotating shaft; the electric heating rod is arranged in the stirring rod; the first bacterium brush is arranged at the front end of the stirring rod. The during operation, the puddler drives a fungus brush and rotates, will paste the photosynthetic bacteria brush of glass jar inner wall away, avoids taking place the spotlight adherence, influences inside photosynthetic bacteria's effective growth, and the suitable temperature of culture solution can be controlled to the electric bar in addition.
Preferably, the acid-base solution module comprises a stirring rod, a second bacteria brush, a detector, a miniature telescopic cylinder and a piston, wherein a hollow cavity is arranged in the stirring rod and communicated with the hollow cavity of the rotating shaft; the second bacteria brush is arranged at the front end of the stirring rod; the detector is arranged on the stirring rod; the micro telescopic cylinder is arranged above the stirring rod; the piston is positioned in the hollow cavity of the stirring rod and is fixedly connected with the telescopic cylinder. During operation, the detector controls the micro telescopic cylinder to work in time according to the acidity of the culture solution in the glass tank, the piston is pushed to extrude calcium-containing solution in the stirring rod, and the pH value of the culture solution is adjusted.
Preferably, the lower surface of the stirring rod is provided with a round hole, a rubber flap is arranged in the round hole, and the rubber flap can be opened under the pressure. The rubber lamella is in the closed condition at ordinary times, and only the inside piston during operation of stirring rod, the rubber lamella pressurized just can open, and the inside acid-base liquid of stirring rod just can be erupted, has effectively avoided the inside nutrient solution of glass jar to enter into in the hollow cavity of stirring rod.
Preferably, the first bacteria brush and the second bacteria brush are weak elastic bristles. When the brush hair collides with the convex block, the brush hair is elastically bent and then restored, the photosynthetic bacteria attached to the brush hair are shaken off, and the using effects of the first bacteria brush and the second bacteria brush are improved.
The invention has the beneficial effects that:
1. the photosynthetic bacteria culture method is simple and practical, the culture period of the photosynthetic bacteria is short, and in the fifth step, the stirring module and the acid-base regulation module are matched with each other, so that the photosynthetic bacteria are uniformly mixed on one hand, the rapid growth of the photosynthetic bacteria is facilitated, and the light condensation and wall adhesion are effectively treated on the other hand, so that the photosynthetic bacteria can obtain sufficient illumination conditions, and meanwhile, the pH value of a culture solution is controlled, a suitable production environment for the photosynthetic bacteria is provided, the culture time is shortened, the cost is saved, and the productivity is improved.
2. The light-emitting plate disclosed by the invention is used for illuminating at 360 degrees, so that the sufficient illumination condition is ensured, and the growth of photosynthetic bacteria is facilitated.
3. The brush hair and the convex block of the first bacteria brush and the second bacteria brush collide with each other, so that photosynthetic bacteria attached to the first bacteria brush and the second bacteria brush can be shaken off, and the working effect of the first bacteria brush and the second bacteria brush is improved.
4. The third step of the invention: the convex lens is arranged in the glass tank, so that light can be condensed and heated by the illumination emitted by the light-emitting plate, a proper temperature is provided, and the propagation and growth of photosynthetic bacteria are accelerated.
5. The power generation panel provided by the invention utilizes solar energy to generate power, so that the energy is saved and the cost is reduced.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of a method of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
in the figure: the device comprises a glass tank 1, a light-emitting plate 2, a power generation plate 3, an acid-base solution tank 4, a motor 5, a rotating shaft 6, a stirring module 7, a stirring rod 71, an electric heating rod 72, a first bacterial brush 73, an acid-base adjusting module 8, a stirring rod 81, a second bacterial brush 82, a micro telescopic cylinder 83, a piston 84, a rotating disc 9, a first motor 10, a feeding hole 11, a discharging hole 12, a convex lens 13 and a bump 14.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 and fig. 2, the method for culturing photosynthetic bacteria of the present invention employs a photosynthetic bacteria culture apparatus, wherein the culture apparatus comprises a glass tank 1, a light-emitting plate 2, a power generation plate 3, an acid-base solution tank 4, a motor 5, a rotating shaft 6, a stirring module 7, an acid-base regulation module 8, a rotating disk 9, a motor one 10, a feeding port 11 and a discharging port 12, the glass tank 1 is in a cylindrical shape, and the glass tank 1 is used for containing a culture medium solution and photosynthetic bacteria; the light-emitting plate 2 is concentrically arranged on the outer side of the glass tank 1; the power generation plate 3 is concentrically arranged on the outer side of the light emitting plate 2; the acid-base solution tank 4 is positioned above the tank cover; the motor 5 is positioned above the acid-base solvent box; the rotating shaft 6 is arranged on the motor 5, and the rotating shaft 6 penetrates through the acid-base solution tank 4; the stirring module 7 is arranged on the rotating shaft 6, and the stirring module 7 is used for stirring photosynthetic bacteria; the acid-base adjusting module 8 is arranged at the upper end of the rotating shaft 6, and the acid-base adjusting module 8 is positioned in the glass tank 1; the rotating disc 9 is positioned below the glass jar 1; the first motor 10 and the rotating disc 9 are fixedly arranged; the feeding hole 11 is positioned at the upper end of the glass tank 1; the discharge hole 12 is positioned at the bottom of the glass tank 1;
the specific method comprises the following steps:
firstly, preparing photosynthetic bacteria culture solution according to the following preparation ratio: 500 g of medium: 80 liters of water: 20 liters of strains;
step two, after the step one is finished, adding the prepared photosynthetic bacteria culture solution into the glass jar 1 through the feed inlet 11;
step three, after the step two is finished, illumination culture: the luminous plate 2 is turned on to provide sufficient illumination energy, the convex lens arranged in the interlayer of the glass tank 1 condenses light and heats the light, and the rotating disc 9 drives the outer layer of the glass tank 1 to rotate to uniformly heat the condensed bacterial liquid;
step four, after the step three is finished, controlling the culture temperature to be 30-38 ℃;
after the fourth step is finished, the motor is started, the stirring rod 71 drives the bacterium brush I73 to rotate, the photosynthetic bacteria attached to the inner wall of the glass tank 1 are brushed away, and the phenomenon that light condensation and wall adhesion occur to influence the effective growth of the photosynthetic bacteria inside is avoided; the detector controls the micro telescopic cylinder 83 to work in time according to the acidity of the culture solution in the glass tank 1, pushes the piston 84 to press out the calcium-containing solution in the stirring rod 81, and adjusts the pH value of the culture;
and step six, after the step five is finished, the photosynthetic bacteria are poured out from the discharge hole 12 after being cultured for 3-5 days.
As an embodiment of the invention, the glass jar 1 is a double-layer glass jar 1, a convex lens 13 is arranged between double-layer glass of the glass jar 1, the convex lens 13 is used for condensing light and increasing temperature, the glass on the outer layer of the glass jar 1 can rotate, and the inner wall of the glass jar 1 is uniformly provided with a bump 14. When the glass jar works, the convex lens 13 condenses light and heats the light-emitting plate 2, adjusts the temperature in the glass jar 1 and promotes the fastest growth of photosynthetic bacteria.
As an embodiment of the invention, the rotating shaft 6 is divided into two sections, the upper section is provided with a hollow chamber which is communicated with the acid-base adjusting module 8 and the acid-base solution tank 4, the lower section is of a solid structure, and the lower section is provided with a thread structure. The lower section of the rotating shaft 6 is in threaded connection with the stirring module 7, the stirring module 7 can move up and down along with the rotating shaft 6, a hollow chamber formed in the upper section of the rotating shaft 6 is communicated with the acid-base adjusting module 8, and acid-base liquid in the acid-base adjusting module 8 can be supplemented timely.
As an embodiment of the present invention, the stirring module 7 includes a stirring rod 71, an electric heating rod 72 and a bacteria brush 73, wherein the stirring rod 71 is mounted on the rotating shaft 6; the electric heating rod 72 is arranged inside the stirring rod 71; the first bacteria brush 73 is arranged at the front end of the stirring rod 71. During operation, puddler 71 drives a 73 rotations of fungus brush, brushes away the photosynthetic bacteria who pastes at glass jar 1 inner wall, avoids taking place the spotlight adherence, influences inside photosynthetic bacteria's effective growth, and the suitable temperature of culture solution can be controlled to electric rod 72 in addition.
As an embodiment of the invention, the acid-base solution module comprises a stirring rod 81, a second bacteria brush 82, a detector, a micro telescopic cylinder 83 and a piston 84, wherein a hollow cavity is arranged in the stirring rod 81 and is communicated with the hollow cavity of the rotating shaft 6; the second bacteria brush 82 is arranged at the front end of the stirring rod 81; the detector is arranged on the stirring rod 81; the micro telescopic cylinder 83 is arranged above the stirring rod 81; the piston 84 is positioned in the hollow cavity of the stirring rod 81, and the piston 84 is fixedly connected with the micro telescopic cylinder 83. When the device works, the detector timely controls the micro telescopic cylinder 83 to work according to the acidity of the culture solution in the glass tank 1, the piston 84 is pushed to extrude the calcium-containing solution in the stirring rod 81, and the pH value of the culture is adjusted.
In one embodiment of the present invention, a circular hole is formed in the lower surface of the stirring rod 81, and a rubber flap is installed in the circular hole and can be opened when being pressed. Rubber lamella is in the closed condition at ordinary times, and only the inside piston 84 during operation of stirring rod 81, rubber lamella pressurized could open, and the inside acid-base liquid of stirring rod 81 just can be erupted, has effectively avoided the inside nutrient solution of glass jar 1 to enter into the hollow cavity of stirring rod 81.
In one embodiment of the present invention, the first bacterial brush 73 and the second bacterial brush 82 are weak elastic bristles. When the bristles collide with the bumps 14, the bristles are elastically bent and then restored, photosynthetic bacteria attached to the bristles are shaken off, and the using effects of the first bacteria brush 73 and the second bacteria brush 82 are improved.
When the device is used, photosynthetic bacteria and culture solution are added from the feeding hole 11, the motor 5 is started, the light-emitting plate 2 is electrified, the motor 5 rotates to drive the stirring module 7 to rotate, the stirring rod 71 drives the bacteria brush I73 to rotate, the photosynthetic bacteria adhered to the inner wall of the glass tank 1 are brushed away, and the phenomenon that light condensation adherence is caused to influence the effective growth of the photosynthetic bacteria in the glass tank is avoided; meanwhile, the acid-base adjusting module 8 also rotates, the detector timely controls the micro telescopic cylinder 83 to work according to the acidity of the culture solution in the glass tank 1, the piston 84 is pushed to extrude the calcium-containing solution in the stirring rod 81, the pH value of the culture is adjusted, the convex lens 13 arranged in the interlayer of the glass tank 1 heats the culture solution, the rotating disc 9 rotates to drive the outer glass tank 1 to uniformly heat, and the culture solution flows out of the discharge hole 12 after the culture is finished.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (2)
1. A photosynthetic bacteria culture method is characterized in that: the method adopts a photosynthetic bacteria culture instrument, wherein the culture instrument comprises a glass tank (1), a light-emitting plate (2), a power-generating plate (3), an acid-base solution box (4), a motor (5), a rotating shaft (6), a stirring module (7), an acid-base adjusting module (8), a rotating disc (9), a motor I (10), a feeding hole (11) and a discharging hole (12), the glass tank (1) is in a cylindrical shape, and the glass tank (1) is used for containing a culture medium solution and photosynthetic bacteria; the light-emitting plate (2) is concentrically arranged on the outer side of the glass tank (1); the power generation board (3) is concentrically arranged on the outer side of the light emitting board (2); the acid-base solution tank (4) is positioned above the tank cover; the motor (5) is positioned above the acid-base solvent box; the rotating shaft (6) is arranged on the motor (5), and the rotating shaft (6) penetrates through the acid-base solution tank (4); the stirring module (7) is arranged on the rotating shaft (6), and the stirring module (7) is used for stirring photosynthetic bacteria; the acid-base adjusting module (8) is arranged at the upper end of the rotating shaft (6), and the acid-base adjusting module (8) is positioned in the glass tank (1); the rotating disc (9) is positioned below the glass tank (1); the first motor (10) and the rotating disc (9) are fixedly installed; the feeding hole (11) is positioned at the upper end of the glass tank (1); the discharge hole (12) is positioned at the bottom of the glass tank (1);
the specific method comprises the following steps:
firstly, preparing photosynthetic bacteria culture solution according to the following preparation ratio: 500 g of medium: 80 liters of water: 20 liters of strains;
step two, after the step one is finished, adding the prepared photosynthetic bacteria culture solution into the glass jar (1) through the feed inlet (11);
step three, after the step two is finished, illumination culture: the luminous plate (2) is turned on to provide sufficient illumination energy, the convex lens arranged in the interlayer of the glass tank (1) condenses light and heats the light, and the rotating disc (9) drives the outer layer of the glass tank (1) to rotate;
step four, after the step three is finished, controlling the culture temperature to be 30-38 ℃;
step five, after the step four is finished, the motor is started, the stirring module (7) and the acid-base adjusting module (8) stir the photosynthetic bacteria culture solution and adjust the pH value, and meanwhile, the adherent of the photosynthetic bacteria is treated;
sixthly, after the fifth step is finished, the photosynthetic bacteria are poured out from the discharge hole (12) after being cultured for 3 to 5 days;
the glass tank (1) is a double-layer glass tank (1), a convex lens (13) is arranged between double layers of glass of the glass tank (1), the convex lens (13) is used for condensing light and increasing temperature, the glass on the outer layer of the glass tank (1) can rotate, and bumps (14) are uniformly arranged on the inner wall of the glass tank (1); the rotating shaft (6) is divided into two sections, the upper section is provided with a hollow cavity, the hollow cavity is communicated with the acid-base adjusting module (8) and the acid-base solution tank (4), the lower section is of a solid structure, and the lower section is provided with a threaded structure; the stirring module (7) comprises a stirring rod (71), an electric heating rod (72) and a first bacterium brush (73), wherein the stirring rod (71) is arranged on the rotating shaft (6); the electric heating rod (72) is arranged in the stirring rod (71); the first bacteria brush (73) is arranged at the front end of the stirring rod (71); the acid-base adjusting module (8) comprises a stirring rod (81), a second bacteria brush (82), a detector, a micro telescopic cylinder (83) and a piston (84), wherein a hollow cavity is arranged in the stirring rod (81) and communicated with the hollow cavity of the rotating shaft (6); the second bacterium brush (82) is arranged at the front end of the stirring rod (81); the micro telescopic cylinder (83) is arranged above the stirring rod (81); the piston (84) is positioned in a hollow cavity of the stirring rod (81), and the piston (84) is fixedly connected with the micro telescopic cylinder (83); the first bacterium brush (73) and the second bacterium brush (82) are weak elastic bristles.
2. A photosynthetic bacteria cultivation method according to claim 1 characterized in that: the lower surface of the stirring rod (81) is provided with a round hole, a rubber flap is arranged in the round hole, and the rubber flap can be opened under the pressure.
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| WO2016208542A1 (en) * | 2015-06-26 | 2016-12-29 | 国立研究開発法人理化学研究所 | Method for producing polyhydroxyalkanoic acid using purple photosynthetic bacterium |
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| CN205473787U (en) * | 2016-01-26 | 2016-08-17 | 浙江浙大水业有限公司 | Culture apparatus of photosynthetic bacteria |
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| CN201915083U (en) * | 2010-09-13 | 2011-08-03 | 江苏邦德生物科技发展有限公司 | Efficient culturing device for photosynthetic bacteria |
| CN201999920U (en) * | 2011-01-18 | 2011-10-05 | 镇江日泰生物工程设备有限公司 | Photosynthetic response fermentation device |
| WO2016208542A1 (en) * | 2015-06-26 | 2016-12-29 | 国立研究開発法人理化学研究所 | Method for producing polyhydroxyalkanoic acid using purple photosynthetic bacterium |
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