CN108251277B

CN108251277B - Microbial cultivation case

Info

Publication number: CN108251277B
Application number: CN201810252053.0A
Authority: CN
Inventors: 郭庆; 汪玮; 张瑞
Original assignee: Qingdao Ama Co ltd
Current assignee: QINGDAO AMA Co.,Ltd.
Priority date: 2018-03-26
Filing date: 2018-03-26
Publication date: 2021-07-02
Anticipated expiration: 2038-03-26
Also published as: CN108251277A

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism incubator which comprises a cylindrical incubator body, an incubator tank, a motor, an ultraviolet lamp and a culture solution conveying pipe; the culture tanks are positioned in the box body, the number of the culture tanks is three, and the culture tanks are used for culturing microorganisms; the motor is arranged at the bottom of the culture tank in the middle; the ultraviolet lamp is arranged on the inner wall of the box body; the culture solution conveying pipe penetrates through the box body, the culture solution conveying pipe is located inside the culture tank, and the culture solution conveying pipe is used for supplementing culture solution for the culture tank. The invention is mainly used for culturing microorganisms in batches, and the culture fluid conveying pipe is utilized to supplement the culture fluid in the culture tank in time, so that the propagation and growth of the microorganisms are improved to the maximum extent; the self-power generation of the stirring rod is utilized to provide the proper temperature in the culture tank; self sterilization can be realized, and the sterilization is more thorough; the mutual meshing of the culture tanks is used as power transmission, so that the use number of motors is reduced.

Description

Microbial cultivation case

Technical Field

The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism incubator.

Background

Microorganisms comprise bacteria, viruses, fungi, some small protozoa and the like, and a large biological population, wherein individuals are tiny, are closely related to human life, and widely relate to a plurality of fields of health, medicine, industry and agriculture, environmental protection and the like. The research on microorganisms has a very positive effect on the daily life of people, and the deep experimental research on the microorganisms is very necessary. In the experimental study of microorganisms, firstly, the microorganisms are cultured, a culture medium is needed for culturing the microorganisms, a specific culture medium is prepared according to different types and life habits of the microorganisms, the key point of successful culture of the microorganisms is aseptic operation, and if culture instruments and the culture medium cannot be sterilized completely and mixed bacteria pollution is generated in the culture process, the failure is easy.

The traditional microbial liquid fermentation mainly adopts shaking table and fermentation tank culture, because of a plurality of types of microbes and different culture temperature and culture time, the conventional shaking table culture and fermentation tank culture can only control a certain specific culture condition, and in addition, the aseptic addition of culture solution in the culture process is difficult to realize by the shaking table culture; in addition, dead angles exist in sterilization, the experiment and production efficiency are greatly reduced, the cultured microorganisms need to be transferred, and the microorganisms are easy to die in the transfer process, so that certain loss is brought to users.

In view of this, the invention provides a microbial incubator, which can control the culture temperature and supplement the culture solution in time.

Disclosure of Invention

In order to make up the defects of the prior art, the invention provides a microorganism incubator which is mainly used for batch culture of microorganisms, and the culture solution conveying pipe is utilized to timely supplement the culture solution in a culture tank, so that the propagation and growth of the microorganisms are improved to the maximum extent; the self-power generation of the stirring rod is utilized to provide the proper temperature in the culture tank; self sterilization can be realized, and the sterilization is more thorough; the mutual meshing of the culture tanks is used as power transmission, so that the use number of motors is reduced.

The technical scheme adopted by the invention for solving the technical problems is as follows: the microbial incubator comprises a cylindrical incubator body, an incubator tank, a motor, an ultraviolet lamp and a culture solution conveying pipe; the culture tanks are positioned in the box body, the number of the culture tanks is three, and the culture tanks are used for culturing microorganisms; the motor is arranged at the bottom of the culture tank in the middle; the ultraviolet lamp is arranged on the inner wall of the box body and is used for sterilizing the incubator; the culture solution conveying pipe passes through the box, the culture solution conveying pipe is located the inside of cultivateing the jar, the culture solution conveying pipe is used for supplementing the culture solution for cultivateing the jar. During operation, ultraviolet lamp carries out sterilization process to box inside, adds the culture solution and adds the microorganism that needs the cultivation in the culture tank, covers the lid, opens the motor, and the culture tank rotates, makes inside microorganism stir the mixing.

Preferably, the culture tank outer wall be equipped with the profile of tooth structure, the meshing of the culture tank on middle culture tank and both sides, the culture tank bottom is equipped with the support column, supports to live to rotate to connect at the bottom half, the inner wall of culture tank is equipped with the puddler.

Preferably, the stirring rod comprises a helical blade, a rotating shaft, a micro generator and a heating rod, a round hole is formed in the front end of the stirring rod, and the rotating shaft is installed in the round hole; the helical blade is arranged on the rotating shaft; the rotating shaft is connected with the micro generator and generates electricity by the rotation of the helical blades; the heating rod is arranged in the stirring rod and is electrically connected with the micro-generator. During operation, the puddler rotates along with the culture tank, and the culture solution passes through the round hole of puddler front end, and the helical blade in the round hole begins to rotate, and helical blade rotates and drives micro motor electricity generation, and micro motor supplies power for the stick that generates heat, and the stick that generates heat heats the culture solution in the culture tank.

Preferably, the culture solution conveying pipe is provided with a plurality of branch pipes, and the branch pipes are used for supplementing the culture solution and stirring and uniformly mixing the microorganisms; the front end of the branch pipe is provided with a round hole, and a rubber plug is arranged in the round hole; a telescopic cylinder and a piston are arranged in the branch pipe; the telescopic cylinder is arranged in the branch pipe; the piston is arranged on the telescopic cylinder. When the culture tank works, the telescopic cylinder works to push the piston to move, and the culture solution in the branch pipe is pushed into the culture tank.

Preferably, the rubber buffer constitute by six lamella sectors, the rubber ring is installed on the right of rubber buffer, the rubber ring is installed in the ring hole, the rubber ring is used for preventing the rubber buffer to inwards open. When the piston pushes the culture solution to move, the rubber stopper is opened, and the culture solution is sprayed out of the branch pipe.

Preferably, the piston further comprises a first support rod, a second support rod and a spring, and the first support rod is arranged on the telescopic cylinder; the second supporting rod is hinged to the first supporting rod, and the other end of the second supporting rod is connected with the piston; one end of the spring is connected to the first support rod, and the other end of the spring is connected to the second support rod; the first supporting rod and the second supporting rod are provided with electromagnets. When the culture tank works, the first support rod and the second support rod of the piston are powered on, the like poles of the electromagnets on the first support rod and the second support rod repel each other, the first support rod and the second support rod prop the piston to be tightly attached to the inner wall of the branch pipe, the telescopic cylinder pushes the piston to push culture solution into the culture tank, when the telescopic cylinder retracts, the first support rod and the second support rod are powered off, the second support rod is close to the first support rod around the pin shaft under the action of the elastic force of the spring, and the piston wall leaves the inner wall of the branch pipe.

The invention has the beneficial effects that:

1. according to the microbial incubator, the culture tanks are meshed through the tooth-shaped structures, only one motor is needed for driving, the device cost is reduced, the production benefit is improved, large-batch microbial culture can be realized, the labor amount of personnel is reduced, the box body can be sterilized by itself, and the external sterilization is not needed.

2. According to the invention, the spiral blade, the heating rod and the micro motor are arranged in the stirring rod, the spiral blade is rotated through the flowing of the culture solution to drive the generator to generate electricity, the generator provides the motor for the heating rod to work, the self-generation heating is realized, an external power supply is not needed, the cost is reduced, meanwhile, the spiral blade can disturb the flow direction of the culture solution, and the effect of stirring and uniformly mixing is improved.

3. The branch pipe in the culture solution conveying pipe supplements the culture solution to the culture tank on one hand, and serves as a stirring rod on the other hand, so that the overall stirring effect of the culture tank is improved, and the rubber plug arranged in the round hole of the branch pipe is opened in one direction, so that microorganisms and the culture solution in the culture tank are prevented from flowing back into the branch pipe.

4. The piston comprises a first support rod, a second support rod and a spring, electromagnets are embedded in the first support rod and the second support rod, and repel each other when the piston is powered on, the culture solution in the support rods is pushed into the culture tank through the telescopic air cylinder, so that the culture solution can be controlled to be added at any time, other mixed bacteria cannot be brought in, the growth of microorganisms in the culture tank is not influenced, nutrient substances are timely provided, and the propagation and growth speed is accelerated.

Drawings

The invention will be further explained with reference to the drawings.

FIG. 1 is a front view of the present invention;

FIG. 2 is an enlarged view of the invention at A;

FIG. 3 is an enlarged view of the invention at B;

FIG. 4 is a side view of the piston of the present invention;

in the figure: the device comprises a box body 1, a culture tank 2, a stirring rod 21, a spiral blade 22, a rotating shaft 23, a heating rod 24, a motor 3, an ultraviolet lamp 4, a culture solution conveying pipe 5, a branch pipe 51, a rubber plug 52, a telescopic cylinder 53, a piston 54, a rubber ring 55, a first support rod 56, a second support rod 57 and a spring 58.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1 to 4, the microbial incubator of the present invention comprises a cylindrical casing 1, a culture tank 2, a motor 3, an ultraviolet lamp 4 and a culture medium delivery pipe 5, wherein the casing 1 is provided with a plurality of holes; the culture tanks 2 are positioned in the box body 1, the number of the culture tanks 2 is three, and the culture tanks 2 are used for culturing microorganisms; the motor 3 is arranged at the bottom of the culture tank 2 in the middle; the ultraviolet lamp 4 is arranged on the inner wall of the box body 1, and the ultraviolet lamp 4 is used for sterilizing the incubator; the culture solution conveying pipe 5 penetrates through the box body 1, the culture solution conveying pipe 5 is located inside the culture tank 2, and the culture solution conveying pipe 5 is used for supplementing culture solution for the culture tank 2. During operation, ultraviolet lamp 4 carries out sterilization process to box 1 inside, adds culture solution to culture tank 2, adds the microorganism of required cultivation, covers the lid, opens motor 3, and culture tank 2 rotates, makes inside microorganism stir the mixing.

As an embodiment of the invention, the outer wall of the culture tank 2 is provided with a tooth-shaped structure, the culture tank 2 in the middle is meshed with the culture tanks 2 at two sides, the bottom of the culture tank 2 is provided with a support column, the support column is rotatably connected to the bottom of the box body 1, and the inner wall of the culture tank 2 is provided with a stirring rod 21.

As an embodiment of the present invention, the stirring rod 21 includes a helical blade 22, a rotating shaft 23, a micro generator and a heating rod 24, a circular hole is opened at the front end of the stirring rod 21, and the rotating shaft 23 is installed in the circular hole; the helical blade 22 is arranged on the rotating shaft 23; the rotating shaft 23 is connected with a micro generator and generates electricity by the rotation of the helical blade 22; the heating rod 24 is arranged in the stirring rod 21, and the heating rod 24 is electrically connected with the micro-generator. During operation, puddler 21 rotates along with culture tank 2, and the culture solution passes through the round hole of puddler 21 front end, and helical blade 22 in the round hole begins to rotate, and helical blade 22 rotates and drives micro motor 3 electricity generation, and micro generator supplies power and gives heating rod 24, and heating rod 24 heats the culture solution in to culture tank 2.

In one embodiment of the present invention, the culture medium conveying pipe 5 is provided with a plurality of branch pipes 51, and the branch pipes 51 are used for supplementing the culture medium and stirring and mixing the microorganisms; a round hole is formed in the front end of the branch pipe 51, and a rubber plug 52 is arranged in the round hole; a telescopic cylinder 53 and a piston 54 are arranged in the branch pipe 51; the telescopic cylinder 53 is arranged inside the branch pipe 51; the piston 54 is mounted on a telescopic cylinder 53. In operation, the telescopic cylinder 53 is actuated to push the piston 54 to move, thereby pushing the culture medium in the branch pipe 51 into the culture tank 2.

In one embodiment of the present invention, the rubber stopper 52 is formed by a six-segment fan shape, a rubber ring 55 is mounted on the right side of the rubber stopper 52, the rubber ring 55 is mounted in the round hole, and the rubber ring 55 is used for preventing the rubber stopper 52 from expanding inwards. When the piston 54 pushes the culture solution to move, the rubber stopper 52 is opened, and the culture solution is ejected from the branch tube 51.

As an embodiment of the present invention, the piston 54 further includes a first support rod 56, a second support rod 57 and a spring 58, wherein the first support rod 56 is mounted on the telescopic cylinder 53; the second support rod 57 is hinged on the first support rod 56, and the other end of the second support rod 57 is connected with the piston 54; one end of the spring 58 is connected to the first support rod 56, and the other end of the spring 58 is connected to the second support rod 57; the first support rod 56 and the second support rod 57 are provided with electromagnets. When the culture medium feeding device works, the first support rod 56 and the second support rod 57 of the piston 54 are electrified, the electromagnets on the first support rod 56 and the second support rod 57 repel each other, the first support rod 56 and the second support rod 57 stretch the piston 54 to be tightly attached to the inner wall of the branch pipe 51, the telescopic cylinder 53 pushes the piston 54 to push the culture medium into the culture tank 2, when the telescopic cylinder 53 retracts, the first support rod 56 and the second support rod 57 are powered off, the second support rod 57 is drawn close to the first support rod 56 around the pin shaft under the elastic force of the spring 58, and the wall of the piston 54 is separated from the inner wall of the branch.

During operation, the ultraviolet lamp 4 sterilizes the inside of the box body 1, culture solution is added into the culture tank 2, microorganisms to be cultured are added, the cover is covered, the motor 3 is started, the culture tank 2 rotates, the stirring rod 21 rotates along with the culture tank 2, the culture solution passes through a round hole in the front end of the stirring rod 21, the helical blade 22 in the round hole starts to rotate, the helical blade 22 rotates to drive the micro motor 3 to generate electricity, the micro motor 3 supplies power to the heating rod 24, and the heating rod 24 heats the culture solution in the culture tank 2; the telescopic cylinder 53 works, a first support rod 56 and a second support rod 57 of the piston 54 are electrified, the electromagnets on the first support rod 56 and the second support rod 57 repel each other, the first support rod 56 and the second support rod 57 prop the piston 54 open and cling to the inner wall of the branch pipe 51, the telescopic cylinder 53 pushes the piston 54 to push the culture solution into the culture tank 2, when the telescopic cylinder 53 retracts, the first support rod 56 and the second support rod 57 are powered off, the second support rod 57 is drawn close to the first support rod 56 around the pin shaft under the elastic force of the spring 58, the wall of the piston 54 leaves the inner wall of the branch pipe 51, and the culture solution is replenished into the culture tank 2 in a circulating reciprocating manner.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A microbiological incubator, characterized in that: the culture tank comprises a tank body (1), a culture tank (2), a motor (3), an ultraviolet lamp (4) and a culture solution conveying pipe (5), wherein the tank body (1) is cylindrical; the culture tanks (2) are positioned inside the box body (1), the number of the culture tanks (2) is three, and the culture tanks (2) are used for culturing microorganisms; the motor (3) is arranged at the bottom of the culture tank (2) in the middle; the ultraviolet lamp (4) is arranged on the inner wall of the box body (1), and the ultraviolet lamp (4) is used for sterilizing the incubator; the culture solution conveying pipe (5) penetrates through the box body (1), the culture solution conveying pipe (5) is positioned inside the culture tank (2), and the culture solution conveying pipe (5) is used for supplementing culture solution to the culture tank (2);

the outer wall of the culture tank (2) is provided with a tooth-shaped structure, the culture tank (2) in the middle is meshed with the culture tanks (2) on the two sides, the bottom of the culture tank (2) is provided with a support column, the support column is rotatably connected to the bottom of the box body (1), and the inner wall of the culture tank (2) is provided with a stirring rod (21);

the stirring rod (21) comprises a helical blade (22), a rotating shaft (23), a micro generator and a heating rod (24), a round hole is formed in the front end of the stirring rod (21), and the rotating shaft (23) is installed in the round hole; the helical blade (22) is arranged on the rotating shaft (23); the rotating shaft (23) is connected with the micro generator and rotates by utilizing the helical blade (22) to generate electricity; the heating rod (24) is arranged in the stirring rod (21), and the heating rod (24) is electrically connected with the micro-generator;

the culture solution conveying pipe (5) is provided with a plurality of branch pipes (51), and the branch pipes (51) are used for supplementing the culture solution on one hand and playing a role in uniformly stirring and mixing microorganisms on the other hand; a round hole is formed in the front end of the branch pipe (51), and a rubber plug (52) is arranged in the round hole; a telescopic cylinder (53) and a piston (54) are arranged in the branch pipe (51); the telescopic cylinder (53) is arranged in the branch pipe (51); the piston (54) is arranged on the telescopic cylinder (53);

the rubber stopper (52) consists of a six-segment fan shape, a rubber ring (55) is arranged on the right side of the rubber stopper (52), the rubber ring (55) is arranged in a round hole, and the rubber ring (55) is used for preventing the rubber stopper (52) from opening inwards;

the piston (54) further comprises a first support rod (56), a second support rod (57) and a spring (58), wherein the first support rod (56) is arranged on the telescopic cylinder (53); the second support rod (57) is hinged to the first support rod (56), and the other end of the second support rod (57) is connected with the piston (54); one end of the spring (58) is connected to the first support rod (56), and the other end of the spring (58) is connected to the second support rod (57); the first support rod (56) and the second support rod (57) are provided with electromagnets.