CN204466643U - A kind of coiling worm mass propgation device - Google Patents
A kind of coiling worm mass propgation device Download PDFInfo
- Publication number
- CN204466643U CN204466643U CN201520274461.8U CN201520274461U CN204466643U CN 204466643 U CN204466643 U CN 204466643U CN 201520274461 U CN201520274461 U CN 201520274461U CN 204466643 U CN204466643 U CN 204466643U
- Authority
- CN
- China
- Prior art keywords
- tissue cultures
- porous aggregate
- coiling
- cultures bottle
- electric mixer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000001519 tissues Anatomy 0.000 claims abstract description 23
- 241000244206 Nematoda Species 0.000 claims abstract description 8
- 239000004743 Polypropylene Substances 0.000 claims 1
- -1 polypropylene Polymers 0.000 claims 1
- 229920001155 polypropylene Polymers 0.000 claims 1
- 230000001488 breeding Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000005660 chlorination reaction Methods 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000004083 survival Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
The utility model relates to a kind of coiling worm mass propgation device.This culture apparatus mainly comprises four parts, tissue cultures bottle cap (1), shaft (2), tissue cultures bottle (3), porous aggregate (4), electric mixer (5), charging aperture (6), described tissue cultures bottle cap is arranged at above described tissue cultures bottle, described electric mixer is connected with described tissue cultures bottle cap, described electric mixer is connected with described shaft one end, and described porous aggregate is connected with the described shaft other end, and described shaft, described porous aggregate are placed in inside described tissue cultures bottle.Cultivate nematode being joined in blake bottle after the medium prepared.The utility model meets the life habit of coiling worm, can the worm of mass propgation coiling easily and the coiling worm of collecting breeding.
Description
Technical field
The utility model relates to the culture apparatus of coiling worm in a kind of water.
Background technology
In drinking water, microfauna pollution problem receives and shows great attention in recent years, and wherein nematode especially receives publicity due to its higher recall rate and anti-chlorination ability.In addition, nematode is also described as the animal monoid of most abundant on the earth, is the indicator organism carrying out natural, ecological health risk assessment and pollution assessment.So be necessary the device developing nematode mass propgation, the experiment of the physiological and ecological characteristic of nematode for deliberation is used.
Have not yet to see the patent about coiling worm culture apparatus.
Utility model content
The purpose of this utility model is to solve coiling worm mass propgation problem, provides a kind of structure simple, easy to operate coiling worm mass propgation device.
The utility model is by tissue cultures bottle cap (1), and shaft (2), tissue cultures bottle (3), porous aggregate (4), electric mixer (5), charging aperture (6) six part forms.Tissue culture flasks of the present utility model selects general clear vial, the size of regulation porous aggregate and the distance of distance at the bottom of blake bottle.Described tissue cultures bottle cap is arranged at above described tissue cultures bottle, described electric mixer is connected with described tissue cultures bottle cap, described electric mixer is connected with described shaft one end, described porous aggregate is connected with the described shaft other end, and described shaft, described porous aggregate are placed in inside described tissue cultures bottle.
The utility model has following feature:
There is the habit of having a liking for living in duct, at incubator curriculum offering porous aggregate, for nematode provides good living environment according to coiling worm.
Porous aggregate, by electric mixer slow circumvolve, produces certain flowing water environment, and coiling worm can be impelled further to survive in porous aggregate and breed.The coiling worm of breeding can be collected thus easily by porous aggregate.
Accompanying drawing explanation
Fig. 1 is coiling worm culture apparatus figure.Wherein, wherein 1-tissue cultures bottle cap, 2-shaft, 3-tissue cultures bottle, 4-porous aggregate, 5-electric mixer, 6-charging aperture.
Embodiment
Embodiment is as follows:
Medium is joined in the tissue cultures bottle in coiling worm mass propgation device, medium submergence porous aggregate.The dischargeable capacity of tissue culture flasks is 1L.
Be cylinder by porous aggregate profile, basal diameter is 8cm, is highly 8cm, and the distance of distance bottom is 0.6cm.The aperture of porous aggregate is 1.5mm.Start agitator and drive porous aggregate slow circumvolve, mixing speed is 10rpm.
With the density of 20/L inoculation coiling worm in tissue cultures bottle.
Coiling worm mass propgation device is put into 25 DEG C of constant incubators, cultivates coiling worm 9 days, then open the lid of tissue culture flasks, take out porous aggregate, with the coiling worm in aseptic water washing porous aggregate, just obtain coiling worm polypide, obtain around nematode population more than 20000.
The survival rate of basis of microscopic observation result display coiling worm reaches more than 98%.
Claims (5)
1. a coiling worm mass propgation device, it is characterized in that: described culture of nematodes device comprises tissue cultures bottle cap, shaft, tissue cultures bottle, porous aggregate, electric mixer, described tissue cultures bottle cap is arranged at above described tissue cultures bottle, described electric mixer is connected with described tissue cultures bottle cap, described electric mixer is connected with described shaft one end, described porous aggregate is connected with the described shaft other end, and described shaft, described porous aggregate are placed in inside described tissue cultures bottle.
2. coiling worm mass propgation device according to claim 1, it is characterized in that, described porous aggregate is made primarily of polypropylene.
3. coiling worm mass propgation device according to claim 1 and 2, is characterized in that, the profile of described porous aggregate is cylinder, and basal diameter is 5cm ~ 20cm, is highly 5 ~ 30cm, and the distance of distance bottom is 0.5cm ~ 1cm.
4. coiling worm mass propgation device according to claim 3, is characterized in that, the aperture of described porous aggregate is 1mm ~ 5mm.
5. coiling worm mass propgation device according to claim 1, is characterized in that, described electric mixer is the electric mixer driving described porous aggregate slow circumvolve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520274461.8U CN204466643U (en) | 2015-04-30 | 2015-04-30 | A kind of coiling worm mass propgation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520274461.8U CN204466643U (en) | 2015-04-30 | 2015-04-30 | A kind of coiling worm mass propgation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204466643U true CN204466643U (en) | 2015-07-15 |
Family
ID=53622386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520274461.8U Expired - Fee Related CN204466643U (en) | 2015-04-30 | 2015-04-30 | A kind of coiling worm mass propgation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204466643U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109152351A (en) * | 2016-05-20 | 2019-01-04 | 恩托普洛特公司 | For cultivating the device and method of invertebrate |
-
2015
- 2015-04-30 CN CN201520274461.8U patent/CN204466643U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109152351A (en) * | 2016-05-20 | 2019-01-04 | 恩托普洛特公司 | For cultivating the device and method of invertebrate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150715 Termination date: 20210430 |