CN102796666A - Sloshing-type micro-dynamic incubator - Google Patents
Sloshing-type micro-dynamic incubator Download PDFInfo
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- CN102796666A CN102796666A CN2012102948531A CN201210294853A CN102796666A CN 102796666 A CN102796666 A CN 102796666A CN 2012102948531 A CN2012102948531 A CN 2012102948531A CN 201210294853 A CN201210294853 A CN 201210294853A CN 102796666 A CN102796666 A CN 102796666A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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Abstract
The invention discloses a sloshing-type micro-dynamic incubator which comprises a case body with a tray disposed in the case body, and also comprises a chute, a motor, a reciprocating motion mechanism, and a motor speed adjusting device; the chute is disposed below the tray; a slide block is disposed at the bottom of the tray and fits with the chute; the slide block is slidingly connected with the chute; the chute is supported by a support; the reciprocating motion mechanism comprises a driven rod and a cylindrical cam; a groove curve is disposed on the surface of the cylindrical cam; one end of the driven rod is connected with the groove of the cylindrical cam, and the other end of the driven rod is fixedly connected with the tray bottom; the cylindrical cam drives the driven rod to reciprocate through rotation, and the driven rod drives the tray to reciprocate in the chute; the cylindrical cam comprises a central shaft, and the motor is connected with the central shaft; the motor speed adjusting device is connected with the motor. According to the invention, the tray performs reciprocating motion with a small amplitude and a low frequency, and thus oocytes or embryos cultured in vitro are in dynamic culture environment so as to simulate a sloshing state of the oocytes or embryos in a maternal body.
Description
Technical field
The present invention relates to biotechnology or reproductive medicine field, relate in particular to a kind of incubator that can dynamically cultivate vitro culture ovocyte or embryo.
Background technology
In animal embryo engineering research or human auxiliary procreation technology, the maturation in vitro of ovocyte, the vitro culture of fertilization or preimplantation embryos is the routine operation flow process in the laboratory, mainly is in incubator, to carry out.
For the ectogenesis quality that improves gamete or embryo, laboratory study mainly concentrates on the optimization of medium component, and cytokine is added, suitable oxygen concn etc.With going deep into of laboratory seminar research, the vitro culture system of embryo or gamete is perfect day by day, various culture condition (gas phases; Temperature) simulates environment (uterus or uterine tube) in the female repro ductive system fully as far as possible; But compare with natural embryonic development in the body, embryo quality and implantation rate can not match in excellence or beauty fully, and reason is that the environment of fetal development in the female reproductive tract is complicated; Uterine tube or intrauterine secreting liquid complicated component provide perfect growing environment to embryo's growth and implantation.In these researchs, the maturation of ovocyte and embryo's cultivation mainly are in the substratum of static state, to carry out.And the maturation of ovocyte in the body; And ovocyte combines after fertilization with sperm in the uterine tube portion of expanding, and the continuation of zygote is grown, and is accompanied by intratubal migration; When morula, get into the uterus; Therefore the ovocyte or the embryo that grow in the body are dynamic migrations, are accompanied by the daily routines of parent, are not to remain static always.Embryo's metabolic waste not only can be in time diluted in dynamic variation, promotes the exchange of embryo's surrounding gas and biomacromolecule material simultaneously.
Someone studies and points out, during in-vitro maturity of porcine oocytes, gives the machinery concussion of certain frequency, though can not promote the nuclear ripe (growing the M II phase) of ovocyte, the growth quality of ovocyte can significantly be promoted.Same principle in human reproduction's medical science, is carried out low-frequency concussion to ectogenetic embryo and is perhaps waved; Make it be in dynamic cultivation conditions; The result shows, can significantly promote embryo's growth quality and can promote embryo's later stage implantation, improves incidence of parturition.Explain that development models is more approaching in this training method and the embryoid body.
Summary of the invention
The technical problem that the present invention will solve provides a kind of fine motion attitude incubator that vitro culture ovocyte or embryo's simulation are rocked in parent.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is: rocking type fine motion attitude incubator, comprise casing, and be provided with pallet in the casing, also comprise chute, motor, reciprocating device and motor speed control device; Chute is located at the pallet below, and the tray bottom is provided with the slide block suitable with chute, and slide block and chute are slidingly connected; Chute is by stent support;
Reciprocating device comprises follower lever and cylindrical cam; The cylindrical cam surface is provided with the groove curve, and follower lever one end is connected with the groove of cylindrical cam, and the other end and the tray bottom of follower lever are affixed; The cylindrical cam revolution drives follower lever and moves back and forth, and follower lever drives pallet and in chute, moves back and forth back and forth;
Cylindrical cam is provided with hub, and motor is connected with hub;
Motor speed control device is connected with motor.
As preferably, also comprise timing unit, the break-make of timing unit control motor power.
As preferably, motor is a reducing motor.
As preferably, motor speed control device is an electrically-actuated throttle device.
As preferably, motor speed control device is a mechanical adjustable speed drive; Motor output shaft is connected with crank through mechanical adjustable speed drive.
As preferably, motor speed control device comprises the control button, and said control button is located at box outer surface.
As preferably, reciprocating mechanism is located at the pallet below.
As preferably, the pallet card is provided with the draw-in groove that is used to settle petridish;
As preferably, draw-in groove is square groove or circular groove or strip groove.
As preferably, also comprise petridish, the petridish bottom is provided with and the suitable convexity of pallet draw-in groove.
The invention has the beneficial effects as follows:
Drive pallet through electric motor driving cam reciprocating device and do small size low frequency to-and-fro movement; Make ovocyte or embryo in vitro culture be in dynamic culture environment; To simulate ovocyte or embryo at the intravital vibrating state of mother; Can significantly promote ovocyte or embryo's growth quality and can promote embryo's later stage implantation, improve incidence of parturition.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.
Fig. 1 is the front view of rocking type fine motion attitude incubator embodiment 1 of the present invention.
Fig. 2 is the vertical view of rocking type fine motion attitude incubator embodiment 1 of the present invention.
Fig. 3 is the pallet disk structure figure of rocking type fine motion attitude incubator embodiment 1 of the present invention.
Among the figure, 1-casing, 101-chamber door, 2-pallet, 201-draw-in groove, 3-slide block, 4-chute, 5-follower lever, 6-cylindrical cam, 601-cam groove, 7-motor, 8-motor setting device, 9-petridish, 10-button.
Embodiment
Embodiment 1:
Fig. 1 is a kind of rocking type fine motion attitude incubator, comprises casing 1, and casing is provided with chamber door 101, is provided with pallet 2, chute 4, motor 7, reciprocating device and motor speed control device 8 in the casing.
Reciprocating device is made up of follower lever 5 and cylindrical cam 6.Be provided with curved cam groove 601 on cylindrical cam 6 surfaces, follower lever 5 one ends are connected with the groove of cylindrical cam, and the other end and the tray bottom of follower lever 5 are affixed.Cylindrical cam 6 revolutions drive follower lever and move back and forth, and follower lever drives pallet and in chute, moves back and forth back and forth.
The output shaft of motor is connected with the hub of cam 6 through shaft coupling, turns round through the electric motor driving cam.Motor 7 is a reducing motor.
The curve shape decision of the groove 601 of cylindrical cam moves back and forth motion characteristicss such as amplitude, and the frequency that moves back and forth is by the rotating speed decision of cylindrical cam 6.
Motor speed control device 8 adopts electrically-actuated throttle device, and electrically-actuated throttle device is connected with motor 7.Can regulate the rotating speed of motor output shaft through electrically-actuated throttle device.
Electrically-actuated throttle device is provided with control with button 10, and button 10 is located at casing 1 outside surface (Fig. 2).
Among Fig. 3, be moved in card when pallet 2 moves for avoiding petridish 9, pallet 2 cards are provided with the draw-in groove 201 that is used to settle petridish 9.Set draw-in groove is square or circular, and square draw-in groove is used to be provided with NUNC four hole petridish, and circular draw-in groove then directly adopts and the consistent shape in 35mm circular petridish bottom.
Present embodiment also comprises timing unit (not marking among Fig. 1), and timing unit is used to control the break-make of motor power, thereby control pallet 2 produces intermittent to-and-fro movement.Timing unit also can through control pallet 2 timed motion with regularly stop, reaching the get up in the night to urinate daytime state of volt of simulation parent.
Present embodiment also has and common CO
2Other structures that incubator is identical.Like C0
2, N
2Access port, but interior detected temperatures of case and CO
2Damping device in the infrared probe of concentration, incubator.
During use, the petridish that ovocyte or embryo are housed is embedded in the draw-in groove on the pallet.Set motor speed with button, and the time that intermittently starts, close chamber door 101 and can begin to cultivate.
Use the button starter motor; Motor 7 drives cylindrical cam 6 revolutions; It is reciprocating that follower lever 5 follows cam upper groove 601; Thereby drive pallet 2 is made same amplitude in chute 4 to-and-fro movement, realize the motion of pallet do with oscillation on small scale form back and forth thus, simulation embryo or gamete are at the intravital rolling flowing mode of mother.
Present embodiment is the CO that routine is used
2Incubator improves; Pallet can carry out dynamic low frequency oscillation on small scale according to frequency or the amplitude set; Give a dynamic culture environment, and then can promote ovocyte or embryo's ectogenesis effect, set up perfect oocyte in vitro maturation or embryo's vitro culture system.
Embodiment 2:
Pallet 2 cards are provided with strip-shaped clamp groove, and employed petridish bottom is provided with the bar shaped raised line suitable with strip groove equally.
Motor speed control device 8 adopts mechanical adjustable speed drive, and motor output shaft is connected with cam mechanism through mechanical adjustable speed drive.Can regulate the rotating speed of motor output shaft 11 through mechanical adjustable speed drive.
Other structures of present embodiment are identical with embodiment 1.
Above-described embodiment of the present invention does not constitute the qualification to protection domain of the present invention.Any modification of within spirit of the present invention and principle, being done, be equal to replacement and improvement etc., all should be included within the claim protection domain of the present invention.
Claims (10)
1. rocking type fine motion attitude incubator comprises casing, is provided with pallet in the said casing, it is characterized in that: also comprise chute, motor, reciprocating device and motor speed control device; Said chute is located at the pallet below, and the tray bottom is provided with the slide block suitable with chute, and slide block and chute are slidingly connected; Chute is by stent support;
Said reciprocating device comprises follower lever and cylindrical cam; Said cylindrical cam surface is provided with the groove curve, and follower lever one end is connected with the groove of cylindrical cam, and the other end and the tray bottom of follower lever are affixed; The cylindrical cam revolution drives follower lever and moves back and forth, and follower lever drives pallet and in chute, moves back and forth back and forth;
Said cylindrical cam is provided with hub, and motor is connected with hub;
Motor speed control device is connected with motor.
2. rocking type fine motion attitude incubator according to claim 1 is characterized in that: also comprise timing unit, the break-make of said timing unit control motor power.
3. rocking type fine motion attitude incubator according to claim 1, it is characterized in that: said motor is a reducing motor.
4. rocking type fine motion attitude incubator according to claim 1, it is characterized in that: said motor speed control device is an electrically-actuated throttle device.
5. rocking type fine motion attitude incubator according to claim 1, it is characterized in that: said motor speed control device is a mechanical adjustable speed drive; Motor output shaft is connected with crank through mechanical adjustable speed drive.
6. rocking type fine motion attitude incubator according to claim 1, it is characterized in that: said motor speed control device comprises the control button, said control button is located at box outer surface.
7. rocking type fine motion attitude incubator according to claim 1 is characterized in that: said reciprocating mechanism is located at the pallet below.
8. according to any one described rocking type fine motion attitude incubator of claim 1-7, it is characterized in that: said pallet card is provided with the draw-in groove that is used to settle petridish.
9. rocking type fine motion attitude incubator according to claim 8 is characterized in that: said draw-in groove is square groove or circular groove or strip groove.
10. according to Claim 8 or 9 described rocking type fine motion attitude incubators, it is characterized in that: also comprise petridish, said petridish bottom is provided with and the suitable convexity of pallet draw-in groove.
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CN201210294853.1A CN102796666B (en) | 2012-08-14 | 2012-08-14 | Sloshing-type micro-dynamic incubator |
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CN201210294853.1A CN102796666B (en) | 2012-08-14 | 2012-08-14 | Sloshing-type micro-dynamic incubator |
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CN102796666A true CN102796666A (en) | 2012-11-28 |
CN102796666B CN102796666B (en) | 2014-07-02 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107899486A (en) * | 2017-12-05 | 2018-04-13 | 郑州郑先医药科技有限公司 | A kind of reciprocating gas bath shaking table |
CN108410701A (en) * | 2018-06-01 | 2018-08-17 | 新洲(武平)林化有限公司 | A kind of microbial method preparation high-purity terpinol shake culture device |
CN108486043A (en) * | 2018-02-02 | 2018-09-04 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
CN109666589A (en) * | 2018-10-24 | 2019-04-23 | 海南医学院 | A kind of dynamic cell culture device |
WO2023213029A1 (en) * | 2022-05-06 | 2023-11-09 | 英诺维尔智能科技(苏州)有限公司 | Culture vessel active oscillation-type sliding table mechanism and shaker incubator |
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2012
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107899486A (en) * | 2017-12-05 | 2018-04-13 | 郑州郑先医药科技有限公司 | A kind of reciprocating gas bath shaking table |
CN108486043A (en) * | 2018-02-02 | 2018-09-04 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
CN108486043B (en) * | 2018-02-02 | 2019-03-19 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
CN108410701A (en) * | 2018-06-01 | 2018-08-17 | 新洲(武平)林化有限公司 | A kind of microbial method preparation high-purity terpinol shake culture device |
CN109666589A (en) * | 2018-10-24 | 2019-04-23 | 海南医学院 | A kind of dynamic cell culture device |
WO2023213029A1 (en) * | 2022-05-06 | 2023-11-09 | 英诺维尔智能科技(苏州)有限公司 | Culture vessel active oscillation-type sliding table mechanism and shaker incubator |
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