CN209449410U - Portable boat-carrying bactogen - Google Patents

Portable boat-carrying bactogen Download PDF

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Publication number
CN209449410U
CN209449410U CN201822136998.4U CN201822136998U CN209449410U CN 209449410 U CN209449410 U CN 209449410U CN 201822136998 U CN201822136998 U CN 201822136998U CN 209449410 U CN209449410 U CN 209449410U
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CN
China
Prior art keywords
carrying
culture
carbon dioxide
bottle
bactogen
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Expired - Fee Related
Application number
CN201822136998.4U
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Chinese (zh)
Inventor
冯媛媛
郭佳
席茂年
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Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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Priority to CN201822136998.4U priority Critical patent/CN209449410U/en
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Publication of CN209449410U publication Critical patent/CN209449410U/en
Expired - Fee Related legal-status Critical Current
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The utility model relates to a kind of portable boat-carrying bactogens, including culture bottle, efflux receiving bottle, sea water medium feedway, appliance for applying carbon dioxide, culture tank and temperature control water circle device, culture tank connects temperature control water circle device, culture bottle is placed in culture tank, the import connection sea water medium feedway and appliance for applying carbon dioxide of culture bottle, the outlet of culture bottle connect efflux receiving bottle.This boat-carrying bactogen technological adaptability is strong, easily operated and a variety of training methods can be arranged according to the different research purposes of researcher.Meet and participates in the special techniques demand such as transporting safely in boat-carrying scientific investigation experiment and the fixation on the scientific investigation ship for being easy to shake.

Description

Portable boat-carrying bactogen
Technical field
The utility model belongs to marine organisms research field, is related to boat-carrying culture technique, especially a kind of portable boat-carrying Bactogen.
Background technique
Due to mankind's activities such as combustion of fossil fuel and deforestations, lead to global atmosphere carbon dioxide (CO2) concentration is fast Speed increases, and then causes climate warming, a series of ecological environment problems such as Ocean acidification.Diatom dinoflagellate cocolith etc. lives in table Phytoplankton in layer seawater, since it can carry out photosynthetic carbon fixation effect, carbon cycle and the adjusting marine eco-environment in ocean In play an important role.Boat-carrying culture apparatus be when going to sea or laboratory to phytoplankton in ocean under the conditions of certain environment Carry out a kind of equipment of simulation culture.
Traditional boat-carrying culture apparatus is mainly made of culture tank, in use, by the marine phytoplankton of required culture and Nutritive salt needed for corresponding marine phytoplankton growth, which is placed in culture tank, carries out batch cultur (disposable investment nutritive salt pair Marine phytoplankton etc. carries out inoculated and cultured, can be used for observing its growth characteristics and collects every oceanography investigation index) or half Continuous culture (when phytoplankton reaches certain density, collects a part of biomass, adds new sea water medium and continue to train It supports, can be used for extending incubation time, carry out multiple sample collection).
But traditional boat-carrying culture apparatus temperature control method is generally straightforward, using being continuously pumped into away boat into culture tank The problem of mode of surface seawater controls cultivating system.And traditional boat-carrying culture apparatus cannot carry out marine phytoplankton (marine phytoplankton fast-growth in the case where there is the optimal conditions of artificial light source and inflation is bred, and nutritive salt is at one end for continuous culture It continuously flows into, the algae solution of certain density is flowed out from the other end, and flow can artificially be controlled according to the purpose of researcher, makes plant of swimming Object long period under the conditions of set environment keeps stable exponential growth, is more suitable for studying phytoplankton to environmental condition Adaptation process in the case where the long period is horizontal).It is floating that traditional boat-carrying culture apparatus limits cultivated ocean to a certain extent Swim plant growth conditions and can incubation time, to related scientific research work have certain limitation.
Utility model content
The purpose of the utility model is to overcome in place of the deficiencies in the prior art, provide one kind and can accurately control to be cultivated Carbon dioxide (CO in marine phytoplankton growth course2) conditions such as concentration, temperature, nutritive salt and illumination and can carry out The portable boat-carrying bactogen continuously cultivated.
The utility model solves its technical problem and adopts the following technical solutions to achieve:
A kind of portable boat-carrying bactogen, including culture bottle, efflux receiving bottle, sea water medium supply dress It sets, appliance for applying carbon dioxide, culture tank and temperature control water circle device, culture tank connects temperature control water circle device, in culture tank Interior placement culture bottle, the import connection sea water medium feedway and appliance for applying carbon dioxide of culture bottle, culture bottle go out Mouth connection efflux receiving bottle.
Moreover, the culture bottle is two, one of connection appliance for applying carbon dioxide, another connection air is supplied To device, two nutrition bottles are all connected with sea water medium feedway and efflux receiving bottle.
Moreover, the sea water medium feedway includes peristaltic pump and sea water medium storage tank, sea water medium storage Tank connects peristaltic pump by silicone tube or teflon pipe, and peristaltic pump connects culture bottle by silicone tube or teflon pipe.
Moreover, the appliance for applying carbon dioxide includes that carbon dioxide richness adds device and carbon dioxide steel cylinder, carbon dioxide Steel cylinder connects carbon dioxide richness by silicone tube and adds device, and carbon dioxide richness adds device to connect culture bottle by silicone tube.
Moreover, the air feeder supply is air pump.
Moreover, the temperature control water circle device includes Arrangement for heating or cooling water in receptacle for live fish and circulation immersible pump, circulation immersible pump is put into culture In slot.
Moreover, rectangular box body is made by transparent plexiglass plate in the culture tank, door-opening type case is installed in top of the box Lid.
Moreover, poly (methyl methacrylate) plate is supported by strengthening frame, carrying handle is installed on strengthening frame.
Moreover, installation lock and knob on case lid.
Moreover, covering medium-sized density net or pad pasting outside poly (methyl methacrylate) plate.
The advantages and positive effects of the utility model are as follows:
1, this boat-carrying bactogen technological adaptability is strong, easily operated and can be according to the different research mesh of researcher The a variety of training methods of setting.
2, this boat-carrying bactogen can be realized dense to halomereid progress temperature control, the control nutritive salt cultivated The corresponding continuous culture such as degree level.Since the airtightness of cultivating system is preferable, under the premise of using trace metal cleaning operation, Culture bottle, connecting pipe are such as gone out into decontamination using stringent pickling, sea water medium is placed in clean lab space, is taken Sample also uses stringent trace metal culture technique, it can also be used to which trace metal and the related culture that phytoplankton interacts are real In testing.
3, this boat-carrying bactogen can be applied to various environmental factors (including temperature, carbonate system, great Liang Ying Support salt (such as nitrogen, phosphorus, silicon) and micronutrient salt (such as trace metal) Jia Fu and limitation) live halomereid mould under regulation In quasi- culture experiment.
4, this boat-carrying bactogen, which can meet, participates in transporting safely in boat-carrying scientific investigation experiment and is being easy to shaking The special techniques demand such as fixation on scientific investigation ship.
Detailed description of the invention
Fig. 1 is the structure and connection schematic diagram of the utility model;
Fig. 2 is the portable container structure schematic diagram of this culture apparatus;
Fig. 3 is structural schematic diagram after this culture apparatus packaging.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing and by specific embodiment, and following embodiment is only retouched The property stated, it is not restrictive, protection scope of this utility model cannot be limited by this.
A kind of portable boat-carrying bactogen, including two culture bottles, 1, two efflux receiving bottle 2, cultivation in sea water Base feedway, air feeder, appliance for applying carbon dioxide, culture tank 8 and temperature control water circle device, put in culture tank Two culture bottles are set, two culture bottles are separately connected sea water medium feedway, one of culture bottle connection air supply Device, another culture bottle connect appliance for applying carbon dioxide, water-bath in the temperature control water circle device control culture tank Temperature.
The sea water medium feedway includes peristaltic pump 6 and sea water medium storage tank 7, and sea water medium storage tank is logical It crosses silicone tube or teflon pipe connection peristaltic pump, peristaltic pump connects culture bottle by silicone tube or teflon pipe.
The appliance for applying carbon dioxide includes that carbon dioxide richness adds device 4 and carbon dioxide steel cylinder 5, carbon dioxide steel cylinder Carbon dioxide richness is connected by silicone tube and adds device, and carbon dioxide richness adds device to connect culture bottle by silicone tube.
The air feeder supply is air pump 3.(alternative uses)
The temperature control water circle device includes Arrangement for heating or cooling water in receptacle for live fish 9 and circulation immersible pump 10, and circulation immersible pump is put into culture tank It is interior.
Culture bottle is provided with two entrances end and outlet end.Two entrances end is set to bottle cap, and one of arrival end is logical Cross silicone tube or teflon pipe be connected with peristaltic pump, peristaltic pump again with the outlet end silicone tube of sea water medium storage tank or spy Teflon tube connection.Outlet end is located at bottleneck, is connected using connector and silicone tube with efflux receiving bottle.
According to experiment needs, efflux can be individually placed at dark place or have in the culture tank of temperature control device.
Another arrival end of culture bottle adds rich device to be connected with silicone tube with air pump or carbon dioxide, and carbon dioxide adds Rich device is connected with carbon dioxide steel cylinder with silicone tube again.The culture bottle for connecting other devices and circulation immersible pump are placed In culture tank, immersible pump is connected with the arrival end of Arrangement for heating or cooling water in receptacle for live fish with plastic pipe, and the outlet end of Arrangement for heating or cooling water in receptacle for live fish and plastic pipe connect It connects, the other end of plastic pipe is put into culture tank.
This portable boat-carrying bactogen, in the base of the original universal boat-carrying culture apparatus with culture tank On plinth, increases culture bottle, air pump, sea water medium storage tank, carbon dioxide and add rich device, carbon dioxide steel cylinder, circulation diving Pump and Arrangement for heating or cooling water in receptacle for live fish.
When using such portable boat-carrying bactogen, peristaltic pump constantly extracts in sea water medium storage tank To set rate input cultivating system in cultivation in sea water basal orientation culture bottle with a certain concentration nutritive salt, with sea water medium Continuous input, the algae solution of certain density flows out from the outlet end of culture bottle.For guarantee cultivating system in institute's Object of Development it is equal Even distribution can shake culture bottle or the additional pneumatic device that is added in a certain time interval artificially to achieve the effect that mixing.It is empty Air pump extracts atmosphere, and constantly into the culture bottle being connected with it, (gas concentration lwevel in current atmosphere is about for input 400ppm), carbon dioxide adds rich device that can provide certain density carbon dioxide air gaseous mixture to the culture bottle being connected with it Body, researcher can add rich device to control the carbon dioxide in gas mixture concentration that it is exported by carbon dioxide.Culture bottle is put It is placed in culture tank, by the water temperature in the adjustable culture tank of Arrangement for heating or cooling water in receptacle for live fish, and then controls halomereid in culture bottle Growth temperature.
In addition, the culture tank in the utility model is allowed to be more suitable for the special of boat-carrying culture experiment by special designing Purposes: it not only can be used as the transport supporting body from land laboratory to scientific surveying ship package unit in transit, but also can expire Foot is fixed on scientific investigation boat deck to be used as temperature control control optical culture culture tank.As shown in Fig. 2, the culture culture tank is served as reasons Rectangular box body made of high-intensitive transparent plexiglass plate, top of the box are installed door-opening type lid 14, split can be opened, In transit, internal setting detachable partition, can add carbon dioxide rich device, Arrangement for heating or cooling water in receptacle for live fish are fixed to be packed into wherein, and equipped with training Bottle storage protection case 11 and water pipe peristaltic pump storage protection case 12 are supported, to keep firm, poly (methyl methacrylate) plate splices position by not Rust steelframe is reinforced, and is carried with there are four and used handle 13, for adopting after carrying and arriving at scientific surveying ship in transit The fixation supporting point carried out with restraint zone.The closed state of the special culture tank is shown in Fig. 3, in transit, uncaps and closes on cabinet Merge prevents from being opened accidentally equipped with lock 15, with there are two knob 16 on door of above uncapping, facilitates unlatching culture tank.To culture apparatus After arriving at scientific surveying ship, after each component is removed and connects into Fig. 1 bactogen, the partition that is configured in transportational process and After chest is also removed, which, which can be firmly fixed on deck, carries out corresponding culture experiment.During the experiment, It can choose medium-sized density net according to intensity of illumination needed for testing or pad pasting is covered in the outside of culture tank or surface has met not With control light demand.
This portable boat-carrying bactogen, technological adaptability is strong, easily operated.Especially it can be achieved on to sea Foreign phytoplankton can control its ring during the growth process during being cultivated according to the different research purpose of researcher Border temperature, gas concentration lwevel, nutrient stratifucation and intensity of illumination.Meanwhile the utility model can also be according to researcher not The halomereid cultivated flexibly continuously is cultivated with research purpose, batch culture or Semi-continuous cultivation etc. it is a variety of not Same training method.In addition, the portable boat-carrying bactogen, which can meet, participates in transporting safely in boat-carrying scientific investigation experiment And the special techniques demand such as fixation on the scientific investigation ship for being easy to shake.
The portable boat-carrying bactogen of the utility model can be composed of, equally above-mentioned various structures Above-mentioned effect can be played.
The portable boat-carrying bactogen of preferred embodiments of the present invention is illustrated above, but It is that the utility model is not limited to above-mentioned specific embodiment, without departing from the scope of the claims, can carries out a variety of Deformation or change.The various modifications and change that the utility model is included within the scope of the claims.

Claims (10)

1. a kind of portable boat-carrying bactogen, it is characterised in that: including culture bottle, efflux receiving bottle, cultivation in sea water Base feedway, appliance for applying carbon dioxide, culture tank and temperature control water circle device, culture tank connect temperature control water circle device, Culture bottle, the import connection sea water medium feedway and appliance for applying carbon dioxide of culture bottle, training are placed in culture tank The outlet for supporting bottle connects efflux receiving bottle.
2. portable boat-carrying bactogen according to claim 1, it is characterised in that: the culture bottle is two A, one of connection appliance for applying carbon dioxide, another connects air feeder, and two nutrition bottles are all connected with seawater training Support base feedway and efflux receiving bottle.
3. portable boat-carrying bactogen according to claim 1 or 2, it is characterised in that: the cultivation in sea water Base feedway includes peristaltic pump and sea water medium storage tank, and sea water medium storage tank is connected compacted by silicone tube or teflon pipe Dynamic pump, peristaltic pump connect culture bottle by silicone tube or teflon pipe.
4. portable boat-carrying bactogen according to claim 1 or 2, it is characterised in that: the carbon dioxide Feedway includes that carbon dioxide richness adds device and carbon dioxide steel cylinder, and carbon dioxide steel cylinder connects carbon dioxide richness by silicone tube Add device, carbon dioxide richness adds device to connect culture bottle by silicone tube.
5. portable boat-carrying bactogen according to claim 2, it is characterised in that: the air feeder Supply is air pump.
6. portable boat-carrying bactogen according to claim 1 or 2, it is characterised in that: the temperature control water follows Loop device includes Arrangement for heating or cooling water in receptacle for live fish and circulation immersible pump, and circulation immersible pump is put into culture tank.
7. portable boat-carrying bactogen according to claim 1 or 2, it is characterised in that: the culture tank by Rectangular box body is made in transparent plexiglass plate, and door-opening type case lid is installed in top of the box.
8. portable boat-carrying bactogen according to claim 7, it is characterised in that: poly (methyl methacrylate) plate passes through reinforcing Frame support, installs carrying handle on strengthening frame.
9. portable boat-carrying bactogen according to claim 7, it is characterised in that: on case lid installation lock and Knob.
10. portable boat-carrying bactogen according to claim 7, it is characterised in that: overcoating in poly (methyl methacrylate) plate Cover medium-sized density net or pad pasting.
CN201822136998.4U 2018-12-19 2018-12-19 Portable boat-carrying bactogen Expired - Fee Related CN209449410U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201822136998.4U CN209449410U (en) 2018-12-19 2018-12-19 Portable boat-carrying bactogen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201822136998.4U CN209449410U (en) 2018-12-19 2018-12-19 Portable boat-carrying bactogen

Publications (1)

Publication Number Publication Date
CN209449410U true CN209449410U (en) 2019-10-01

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Application Number Title Priority Date Filing Date
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Country Status (1)

Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111642451A (en) * 2020-05-29 2020-09-11 中国极地研究中心 Shipborne experiment in-situ culture device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111642451A (en) * 2020-05-29 2020-09-11 中国极地研究中心 Shipborne experiment in-situ culture device
CN111642451B (en) * 2020-05-29 2023-08-18 中国极地研究中心 On-board experiment normal position culture apparatus

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