CN102660451B - Horizontal immersion cover type carbon supplement device and carbon supplement method used in culture of microalgae in open pond - Google Patents
Horizontal immersion cover type carbon supplement device and carbon supplement method used in culture of microalgae in open pond Download PDFInfo
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- CN102660451B CN102660451B CN201210138845.8A CN201210138845A CN102660451B CN 102660451 B CN102660451 B CN 102660451B CN 201210138845 A CN201210138845 A CN 201210138845A CN 102660451 B CN102660451 B CN 102660451B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/18—Open ponds; Greenhouse type or underground installations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Abstract
The invention relates to the field of microalgal culture, in particular to a horizontal immersion cover type carbon supplement device and a carbon supplement method used in the culture of microalgae in an open pond. The horizontal immersion cover type carbon supplement device used in the culture of the microalgae in the open pond comprises more than one or two covers (1) and gas distributors (2), wherein the gas distributors (2) are arranged at the bottom of the open pond, the covers (1) cover the gas distributors (2), each cover (1) is a cylinder with two open ends and is arranged along the flow direction of a culture solution, and openings at two ends of each cover (1) are a culture solution inlet (3) and a culture solution outlet (4) respectively; and the distance between each gas distributor (2) and the culture solution outlet (4) of each cover (1) is more than 40 cm. When microalgal cells are cultured in the open pond, carbon dioxide can be effectively utilized for carbon supplement, so that the production cost is greatly reduced.
Description
Technical field
The present invention relates to micro-algae and cultivate field, particularly, the present invention relates to cultivate the horizontal submergence bell-type carbon compensator of micro-algae and mend carbon method for open pond.
Background technology
Micro-algae can produce multi-chemical by photosynthesis stabilizing carbon dioxide.What have can produce aliphatic hydrocarbon, as grape algae produces hydrocarbon amount, can reach 15%～75% of dry cell weight, the accumulated glycogen having, and the accumulated glycerine having, many micro-algaes can reach the more than 60% of dry weight containing grease.The biomass fuel calorific value that algae pyrolysis obtains is on average up to 33MJ/kg.Micro-algae can be cultivated in seawater, buck or half buck,, with farm crop contention soil and Freshwater resources, can also not utilize waste water, is the important channel that shortage of fresh water, desert, soil obtain effective Biological resources.Micro-algae is expected to become following energy and the important sources of chemical.
In microalgae cell, the content of carbon accounts for the over half of its dry cell weight, and the moiety that frustule is self by photosynthesis by carbon dioxide fixation in process of growth, therefore need keep the supply of carbon source in algae culture process in nutrient solution.Inorganic carbon source in algae culture liquid is with HCO
3 -, CO
3 2-with free CO
2three kinds of forms exist.The content ratio of the carbon of three kinds of forms in the aqueous solution changes with the variation of pH value.If use sodium bicarbonate (NaHCO
3) be carbon source, along with HCO
3 -dissociate and CO
2utilization, the pH value of nutrient solution raises gradually, has the NaHCO over half
3be converted into Na
2cO
3and can not be utilized, carbon source consumption is large, and nutrient solution pH raises and causes water body to be difficult to recycle.If directly with CO
2for carbon source, that micro-algae utilizes is exactly CO
2, the problem that can avoid medium pH value to raise, is conducive to the culture environment that remains suitable, and water can for a long time or be reused.
Open cultivation is tradition and simple micro-algae training mode, also be at present by acknowledged for being ripe micro-algae culture technique, advantage is that structure is simple, easy and simple to handle, in the suitability for industrialized production of spirulina, chlorella and salt algae, obtained application (Chaumont D., J.Appl.Phycol., 1993,5:593-604; Richmond A., Progress in Physiological Research, Vol.7, Biopress, Bristol., 1990,269-330; Borowitzka L.T., Bioresource Technology, 1991,38:251-252).Yet the open pond of tradition is due to the liquid layer degree of depth 20～30cm, if directly add CO in cultivation pool in the mode of bubbling
2, because bubble residence time in nutrient solution is short, make CO
2specific absorption very low, only have 13%～20% CO
2be absorbed (Becker EW, Microalgae:biotechnology and microbiology.Cambridge University Press, Cambridge, 1994, pp293).
(the Ferreira B S such as Ferreira, Fernandes H L, Reis A and Mateus M.Microporous hollow fibres for carbon dioxide absorption:mass transfer model fitting and the supplying of carbon dioxide to microalgae cultures.Journal of Chemical Technology and Biotechnology, 1998,71:61-70) utilize hollow-fibre membrane to strengthen gas-liquid mass transfer, to improve CO
2specific absorption, but this method cost is high, hollow-fibre membrane vulnerable to pollution.
Gas hood method (Lee's noctilucence, Hu Hongjun, Zhang Liangjun, Chen Zhixiang.Take the research of carbonic acid gas as carbon source suitability for industrialized production spirulina Technology.Wuhan phytology research, 1996,14 (4): be 349-356) on the both culturing microalgae water surface, to detain the cover of several square meters, carbon dioxide is passed in cover, the water surface that relies on cover to fasten transmits carbonic acid gas to nutrient solution.The problem of the method is that the specific surface area of gas-liquid exchange is little; Thereby in gas hood, meeting build-up of oxygen, nitrogen reduce rate of mass transfer, need continuous emptying, thus the carbonic acid gas in loss gas hood; For the source of the gas containing low concentration of carbon dioxide, the specific absorption of carbonic acid gas is very low; And gas hood internal pressure when slightly high gas can through the liquid level gas hood, spill from gas hood below.Slot type is mended carbon (CN200610018771.9, the device of supplementing CO 2 to micro-algae culture pond) be to dig a deep trouth at cultivation pool side, make nutrient solution flow through deep trouth, at bottom land, arrange ventpipe, to nutrient solution supply carbonic acid gas, the method can be upset the space layout in traditional open pond, and in groove, lacks mixing, the dead band that just becomes mass transfer after the bottom of for some time pit is saturated by carbonic acid gas, has lost the effect of deep trouth.
Cong Wei etc. (CN200510126465.2 is for carbon compensator and using method and the purposes of large-scale culturing micro-algae) have invented in open pond directly for nutrient solution supplements CO
2trap formula carbon compensator, make nutrient solution form circulation in trap formula carbon compensator, significantly extended gas-liquid contact time, and from the bottom gas-feeding of trap formula carbon compensator, increased substantially CO
2specific absorption.But this carbon compensator has increased the resistance to flow of open pond inner fluid, cause keeping the power consumption of impeller-driven fluid under same flow condition to increase, the quantities of construction is bigger than normal in addition.
Summary of the invention
The object of the invention is to, for addressing the above problem, provide a kind of horizontal submergence bell-type carbon compensator of cultivating micro-algae for open pond.
Another object of the present invention is to provide a kind of benefit carbon method of cultivating micro-algae for open pond.
Of the present invention being contemplated that: directly establish gas distributor at open pond base fabric, in order to extend gas-liquid contact time, above gas distributor, arrange and be immersed in the cover in nutrient solution, cover fully extends along nutrient solution flow direction, bubble is enclosed in cover, while together flowing in cover with nutrient solution, there is sufficient duration of contact, thereby improve the specific absorption of gas.
The horizontal submergence bell-type carbon compensator of cultivating micro-algae for open pond of the present invention, comprise 1 or 2 above cover 1 and gas distributors 2, described gas distributor 2 is arranged at bottom, open pond, described cover 1 covers in gas distributor 2 tops, cover 1 is the cylindrical shell of both ends open, along nutrient solution flow direction, arrange, the two-port of cover 1 is respectively nutrient solution import 3 and nutrient solution outlet 4; Wherein, described gas distributor 2 exports 4 distances more than 40 centimetres with the nutrient solution of cover 1.
According to carbon compensator of the present invention, preferred gas sparger 2 and 60～750 centimetres of nutrient solution outlet 4 distances.For easy construction and guarantee enough carbon dioxide absorption rates, cover 1 should guarantee that along the length of nutrient solution flow direction gas-liquid contact time was at 3 seconds～15 seconds, so the distance of the preferred nutrient solution outlet 4 from gas distributor 2 to cover 1 is nutrient solution mobile distances in 3 seconds～15 seconds in runner.In general open pond, the flow velocity of nutrient solution is 20～50 cels, so the distance of the preferred nutrient solution outlet 4 from gas distributor 2 to cover 1 is 60～750 centimetres.
According to carbon compensator of the present invention, wherein, at the bottom of described cover 1 is directly fixed on open pond or built on stilts be arranged at open pond at the bottom of.
According to carbon compensator of the present invention, wherein, it can be also enclosed construction that the bottom of described cover 1 can be opened wide.
According to carbon compensator of the present invention, wherein, main body semicircular in shape cylindrical shell, arc cylindrical shell, rectangle cylindrical shell or the trapezoidal cylindrical shell of described cover 1.
According to carbon compensator of the present invention, in order to reduce the fluid flow resistance in cover 1, the nutrient solution import 3 of described cover 1 and/or nutrient solution outlet 4 can be taper or flaring flaring structure.
According to carbon compensator of the present invention, wherein, described cover 1 is highly 2～20 centimetres, to be as the criterion by nutrient solution submergence.
According to carbon compensator of the present invention, wherein, described cover 1 width is more than or equal to 2 centimetres.The width of each cover may diminish to 2 centimetres (as covering a gas distribution tube), greatly to the width (as covering many gas distribution tubes or a plurality of gas distributor) that approaches open pond.
According to carbon compensator of the present invention, the gas distribution face (plate) of described gas distributor is porous material, can be tubular gas sparger or is associated in the one or more gas distribution head on pneumatic tube.Can be hardware, as porous ceramic pipe or glass core, or software, as commercially available aeration flexible pipe or variable orifice aeration flexible pipe.
According to carbon compensator of the present invention, wherein, described 2 above covers 1 can conjunctedly be processed, and form conjuncted structure.
The wall thickness of described cover 1 is 1 millimeter～10 millimeters.
Described cover material can be the materials such as plastic plate, stainless steel plate, plank, can machine-shaping, have some strength; Can be for transparent or opaque.
The benefit carbon method of cultivating micro-algae for open pond based on above-mentioned carbon compensator of the present invention, is characterized in that, horizontal submergence bell-type carbon compensator is arranged to bottom, open pond along open pond runner direction, makes nutrient solution submergence cross cover top; Nutrient solution nutrient solution import 3 from cover 1 under the promotion of the original agitator 9 in open pond flows into, the CO emitting with gas distributor in carbon compensator
2 nutrient solution outlet 4 from cover 1 after contact is flowed out.
Nutrient solution flow velocity in described open pond is 20～50 cels.
CO in described horizontal submergence bell-type carbon compensator
2flow, amounts to the pure CO of the normal conditions
2, be 0.1～20 liter/min of every meter of open pond width.
In described open pond, flowing of nutrient solution can rely on the original stirrer-driven in open pond to realize, and as impeller or agitating arm, the material of agitator can be bamboo chip, plastics, stainless steel, other metallic substance etc.
In described open pond, the degree of depth of nutrient solution can be 2～30 centimetres.
According to benefit carbon method of the present invention, when the degree of depth of the nutrient solution in the open pond adopting is more shallow, the original agitator in open pond can be dropped to the position of agitator blade tip at the bottom of lower than open pond, that is, agitator 9 blade tips are lower than at the bottom of opening pond.
According to benefit carbon method of the present invention, when the degree of depth of the nutrient solution in the open pond adopting is more shallow, plan in open pond can be laid to the territory, base area, pond of carbon compensator and dig out shallow slot to lay carbon compensator, that is, carbon compensator is installed on lower than the position at the bottom of open pond.
The bottom of the described shallow slot of laying carbon compensator can be flat, flat with fillet, can be also Semi round bottom; Material is cement, plastic plate, stainless steel plate, brick or with material same at the bottom of open pond etc.
When the profile of gas distributor is while being microscler, preferred gas distributor laying mode is that the direction cloth of the length of gas distributor is made as consistent with nutrient solution flow direction.
According to benefit carbon method of the present invention, wherein, described CO
2for purifying flue gas, industrial CO
2gas, pure CO
2gas or be mixed with CO
2airborne one or more or liquid CO
2.
For cultivating the substratum of micro-algae, can be the substratum that is applicable to arbitrarily micro algae growth well known in the art, as Zarrouk substratum, SM substratum, ASP
2substratum, BG-11 substratum etc., also can need CO for certain algae special requirement, culturing process
2substratum.
Horizontal submergence bell-type carbon compensator of the present invention and benefit carbon method can be used in open pond supplements CO in the various micro-algae processes of large scale culturing
2, comprise various micro-algaes such as spirulina, grid algae, Haematocoocus Pluvialls, salt algae, chlorella, chlamydomonas.
In open pond, cultivate in micro-algae process, use CO
2while mending carbon, the low-down major cause of utilization of carbon source rate is that cultivation liquid layer is superficial, and gas-liquid contact time is short, and carbon dioxide has little time to be absorbed and overflows.Carbon compensator of the present invention has not only overcome above-mentioned shortcoming, and than gas hood method (Lee's noctilucence, Hu Hongjun, Zhang Liangjun, Chen Zhixiang.Take the research of carbonic acid gas as carbon source suitability for industrialized production spirulina Technology.Wuhan phytology research, 1996,14 (4): 349-356) assimilated efficiency is high, than slot type, mend carbon (patent CN200610018771.9) easy to operate, than trap formula carbon compensator (patent CN200510126465.2), reduced fluid flow resistance, saved energy consumption.The present invention, while cultivating microalgae cell in open pond, can effectively utilize carbonic acid gas to mend carbon, greatly reduces production cost.
Accompanying drawing explanation
Fig. 1 is the horizontal submergence bell-type carbon compensator schematic diagram of cultivating micro-algae for open pond of the present invention.
Fig. 2 is the position view of the horizontal submergence bell-type carbon compensator of cultivating micro-algae for open pond of the present invention in the runner of open pond.
Fig. 3 be the horizontal submergence bell-type carbon compensator of cultivating micro-algae for open pond of the present invention while using along nutrient solution flow direction view.
Side-view when Fig. 4 is horizontal submergence bell-type carbon compensator use of cultivating micro-algae for open pond of the present invention.
Fig. 5 be carbon compensator cover of the present invention main body different cross section shape schematic diagram (show cover directly drop on open pond at the bottom of, show the cross-sectional shape that has one or more gas distributors under each cover, shows cover).
Fig. 6 is the different set-up mode schematic diagram (show cover make somebody a mere figurehead, show cover bottom-open or sealing, show that cover is conjuncted, show that gas distributor lands or built on stilts) of carbon compensator of the present invention.
Fig. 7 is that the cover of carbon compensator of the present invention adopts flaring structure schematic diagram.
Fig. 8 when to be carbon compensator of the present invention more shallow for the degree of depth of nutrient solution and the agitator of agitator blade tip at the bottom of lower than open pond be used in conjunction with schematic diagram.
Fig. 9 is that carbon compensator of the present invention is installed on the view along nutrient solution flow direction lower than the position at the bottom of open pond.
Figure 10 is that carbon compensator of the present invention is installed on lower than the position side view at the bottom of open pond.
Figure 11 is that carbon compensator of the present invention is cultivated the total arrangement schematic diagram (vertical view) of micro-algae for open pond.
Figure 12 is the system schematic of utilizing carbon compensator automatic makeup carbon of the present invention.
Reference numeral
1, cover 2, gas distributor 3, nutrient solution import 4, nutrient solution outlet
5, nutrient solution flow direction 6, bottom, open pond 7, open pool wall 8, micro algae culturing liquid liquid level
9, agitator 10, arc container 11, shallow slot 12, pH sensor
13, control device 14, CO
2source of the gas 15, topworks 16, under meter
17, tensimeter
Embodiment
The horizontal submergence bell-type carbon compensator of cultivating micro-algae for open pond of the present invention, comprise 1 or 2 above cover 1 and gas distributor 2(Fig. 1, Fig. 6), described gas distributor 2 is arranged at bottom, open pond 6, described cover 1 covers in gas distributor 2 tops, this cover 1 is the cylindrical shell of both ends open, along nutrient solution flow direction 5, arrange, two-port is respectively nutrient solution import 3 and nutrient solution outlet 4(Fig. 2, Fig. 3); Wherein, described gas distributor 2 exports 4 distances more than 40 centimetres with the nutrient solution of cover 1.Described gas distributor 2 exports preferably 60～750 centimetres of 4 distances with the nutrient solution of cover 1.At the bottom of cover 1 can be directly fixed on open pond (Fig. 5) or built on stilts be arranged at open pond at the bottom of (Fig. 6 omits bracing or strutting arrangement), it can be also enclosed construction (Fig. 6) that cover 1 bottom can be opened wide.The main body of cover 1 can semicircular in shape cylindrical shell, arc cylindrical shell, rectangle cylindrical shell or trapezoidal cylindrical shell (Fig. 5).In order to reduce the fluid flow resistance in cover, the nutrient solution import 3 of described cover 1 and/or nutrient solution outlet 4 can be taper or flaring flaring structure (Fig. 7).Cover 1 is highly 2～20 centimetres, to be as the criterion by 8 submergences of micro algae culturing liquid liquid level.The width of cover 1 may diminish to 2 centimetres (as covering a gas distribution tube), can be large enough to approach the width (being the distance of the open pool wall 7 in runner both sides) (as covering many gas distribution tubes or a plurality of gas distributor) of open pond runner.Described 2 above covers 1 can conjunctedly be processed, and form conjuncted structure (Fig. 6).
Benefit carbon method of cultivating micro-algae for open pond of the present invention, it is characterized in that, in open pond, along runner direction, one or more groups horizontal submergence bell-type carbon compensator is set, every group comprises one or more horizontal submergence bell-type carbon compensators, each horizontal submergence bell-type carbon compensator is containing a cover along nutrient solution flow direction and the one or more gas distributors that covered by it, gas distributor is preferably arranged on nutrient solution import 3 places of cover, total arrangement is shown in Figure 11, the group number of horizontal submergence bell-type carbon compensator, every group of number containing horizontal immersion hood formula carbon compensator, the number of the gas distributor under each cover is according to the benefit carbon speed of single horizontal submergence bell-type carbon compensator, the size in open pond, the growth velocity of Object of Development and processing requirement and set.When nutrient solution flows under the promotion of agitator (the conventional agitator using in this area is impeller) in open pond, part nutrient solution is flowed in horizontal submergence bell-type carbon compensator by the nutrient solution import 3 of cover, the carbonated gas that is enclosed in cover below of emitting with gas distributor contacts, the flow through space of cover below, by the nutrient solution outlet 4 of horizontal submergence bell-type carbon compensator, flowed out again, so greatly extended gas-liquid contact time; On the other hand, carbonated gas, by after gas distributor, becomes very little bubble, and gas-to-liquid contact area sharply increases, and has so just greatly improved the specific absorption of carbonic acid gas.
Flowing of nutrient solution can rely on the original stirrer-driven in open pond to realize, and as impeller or agitating arm, the material of agitator can be bamboo chip, plastics, stainless steel, other metallic substance etc.
Nutrient solution flow velocity in open pond while using this horizontal submergence bell-type carbon compensator is generally 20～50 cels, CO
2flow (is amounted to the pure CO of the normal conditions
2) be on every meter of open pond width 0.1～20 liter/min.
The degree of depth of the nutrient solution in described open pond can be 2～30 centimetres.
When the degree of depth of the nutrient solution in the open pond adopting is more shallow, the original agitator 9 in open pond can be arranged on to the top of arc container 10, agitator blade tip lower than opening pond at the bottom of (Fig. 8); Plan in open pond can be laid to the territory, base area, pond of carbon compensator and dig out shallow slot 11 to lay carbon compensator, that is, carbon compensator is installed on lower than the position (Fig. 9, Figure 10) at the bottom of open pond.The bottom of the described shallow slot of laying carbon compensator 11 can be flat, flat with fillet, can be also Semi round bottom (Fig. 9); Material is cement, plastic plate, stainless steel plate, brick or with material same at the bottom of open pond etc.
As shown in figure 11, in racetrack cultivation pool (raceway pond is called for short in modal a kind of open pond), carry out SPIRULINA CULTIVATION.70 meters of raceway pond runner girths, wide 3 meters, agitator 9 is 4 groups of steel impellers that driven by a rotating shaft, each impeller has 4 blades, space an angle of 90 degrees, the staggered miter angle of blade of adjacent impeller, rotating shaft is driven by alternating current machine and step-down gear, and the rotation radius of agitator 9 is 50 centimetres.
In raceway pond, along runner direction, one group 6 horizontal submergence bell-type carbon compensators are set, each horizontal submergence bell-type carbon compensator is containing a cover 1 along nutrient solution flow direction and the gas distributor 2 being covered by it.Each cover is made by the stainless steel plate of 1 mm thick, and the main body section of cover is shaped as semicircle, and 400 centimetres of each cover length are wide 10 centimetres, high 5 centimetres.Gas distributor 2 is mipor rubber film aeration tube, long 50 centimetres, 16 millimeters of its external diameters, and 10 millimeters of internal diameters, aperture is about 30～60 microns, is arranged between 0～50 centimetre of the nutrient solution import of cover below, radial position is placed in the middle along nutrient solution flow direction.Nutrient solution is promoted along flow passage by agitator 9, part nutrient solution enters the nutrient solution import 3 of carbon compensator, the bubble of emitting with gas distributor 2 contacts, the flow through space of cover 1 below, the nutrient solution outlet 4 of flowing out carbon compensator, runner in continues to circulate after mixing with the nutrient solution that does not enter carbon compensator.
Mend carbon and adopt control automatically, autocontrol method is shown in the patent of application number CN200410009360.4, and embodiment system is referring to Figure 12.Wherein, pH sensor 12 is commercially available pH electrode, the pH meter that control device 13 is controlled for belt switch, CO
2source of the gas 14 is the pure carbon dioxide from steel cylinder, and topworks 15 is two normally closed solenoid valves (8 millimeters of latus rectums).According to the pH value of nutrient solution, control the open and close of carbon dioxide valve (topworks 15).CO
2the flow of gas and pressure are by under meter 16 and tensimeter 17 monitorings.
Algae kind is spirulina plalensis (Spirulina Platensis), from Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse, and numbering 439, substratum is Zarrouk substratum, wherein the starting point concentration of sodium bicarbonate is 0.05mol/L.In raceway pond, nutrient solution mean depth is 15 centimetres, frustule inoculum density 0.40g (dry weight)/L.The span of control of pH is set as 9.6～9.7, starts automatic makeup carbon when the pH of nutrient solution is elevated to 9.6, and the flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 1 liter/min (pure carbon dioxide, mark condition).It is 25 cels left and right that the rotating speed of adjusting motor makes the flow velocity of nutrient solution in raceway pond.
Regularly detect the concentration of other nutritive salt every day and supplement in time, and supplementing a small amount of water to make up evaporation of water loss.Continue to cultivate 6 days, algae cell density reaches 0.70g (dry weight)/L, and the output of unit surface frustule reaches 13.3g (dry weight)/m
2.d.The composition of conventional ingredient, amino acid, lipid acid and the carotenoid of acquisition spiral algae powder and content and bibliographical information are basically identical.Through material balance, show that the utilization ratio of steel cylinder carbonic acid gas is 92%.
Other are with embodiment 1.In open pond, cultivate chlorella, nutrient solution is with NaNO
3bG-11 substratum for nitrogenous source.In raceway pond, nutrient solution mean depth is 12 centimetres.Frustule inoculum density 0.5g (dry weight)/L.The span of control of pH is set as 7.5～7.6.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 0.5 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 30 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 90%.
Other are with embodiment 1.800 centimetres of each cover length, wide 6 centimetres, high 3 centimetres.In raceway pond, nutrient solution mean depth is 10 centimetres.Frustule inoculum density 0.6g (dry weight)/L.The span of control of pH is set as 9.5～9.6.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 1 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 50 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 98%.
Other are with embodiment 1.One group 3 horizontal submergence bell-type carbon compensators are set, and each horizontal submergence bell-type carbon compensator is containing 1 cover along nutrient solution flow direction and 4 gas distributors that covered by it.The main body section of cover is shaped as arc, and 200 centimetres of each cover length are wide 40 centimetres, high 10 centimetres.In open pond, cultivate chlorella, nutrient solution is with NaNO
3bG-11 substratum for nitrogenous source.In raceway pond, nutrient solution mean depth is 20 centimetres.Frustule inoculum density 0.3g (dry weight)/L.The span of control of pH is set as 7.5～7.6.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 0.5 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 25 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 86%.
Embodiment 5.
Other are with embodiment 1.Below agitator, dig out radius and be the semicircular arc groove of 10 centimetres of 52 centimetres, the degree of depth, the blade tip of agitator is dropped to lower than at the bottom of raceway pond 8 centimetres.Each horizontal submergence bell-type carbon compensator is containing 1 cover along nutrient solution flow direction and 2 gas distributors that covered by it.The main body section of cover is shaped as arc, and 200 centimetres of each cover length are wide 15 centimetres, high 3 centimetres.The shallow slot on 2 centimetres of dark flat slopes is dug out in the territory, base area, pond of each carbon compensator below, and each cover and gas distributor are directly arranged at flat upper (Fig. 9, Figure 10) of shallow slot.In open pond, cultivate grid algae, nutrient solution is with NaNO
3bG-11 substratum for nitrogenous source.In raceway pond, nutrient solution mean depth is 5 centimetres.Frustule inoculum density 1.2g (dry weight)/L.The span of control of pH is set as 7.4～7.5.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 0.5 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 25 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 85%.
Other are with embodiment 1.Below agitator, dig out radius and be the semicircular arc groove of 10 centimetres of 52 centimetres, the degree of depth, the blade tip of agitator is dropped to lower than at the bottom of raceway pond 8 centimetres.Each horizontal submergence bell-type carbon compensator is containing 1 cover along nutrient solution flow direction and 2 gas distributors that covered by it.The main body section of cover is shaped as arc, and 200 centimetres of each cover length are wide 10 centimetres, high 2 centimetres.The shallow slot on 3 centimetres of dark flat slopes is dug out in the territory, base area, pond of each carbon compensator below, built on stilts 1 centimetre of flat upper (Fig. 9, Figure 10, the Fig. 6) that is arranged at shallow slot of each cover and gas distributor.In raceway pond, nutrient solution mean depth is 3 centimetres.Frustule inoculum density 2g (dry weight)/L.The span of control of pH is set as 9.7～9.8.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 0.5 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 25 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 88%.
Other are with embodiment 1.The main body section of cover is shaped as trapezoidal, and 100 centimetres of each cover length are wide 15 centimetres, high 15 centimetres.Gas distributor is the variable orifice aeration flexible pipe of diameter 65mm, long 40 centimetres, is arranged between 0～40 centimetre of the nutrient solution import of cover below along nutrient solution flow direction.In raceway pond, nutrient solution mean depth is 25 centimetres.Frustule inoculum density 0.24g (dry weight)/L.The span of control of pH is set as 9.8～9.9.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 1 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 20 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 79%.
Other are with embodiment 1.Each horizontal submergence bell-type carbon compensator is containing 1 cover along nutrient solution flow direction and 2 gas distributors that covered by it.The main body section of cover is shaped as rectangle, and 50 centimetres of each cover length are wide 20 centimetres, high 20 centimetres.Gas distributor is the core of 30 millimeters of diameters, high 70 millimeters, perpendicular to nutrient solution flow direction, is arranged side by side between 5～10 centimetres of nutrient solution imports below cover.In raceway pond, nutrient solution mean depth is 30 centimetres.Frustule inoculum density 0.2g (dry weight)/L.The span of control of pH is set as 9.9～10.0.The flow of the carbon dioxide of each gas distributor (topworks 15 opens) is 0.5 liter/min (pure carbon dioxide, mark condition).In raceway pond, the flow velocity of nutrient solution is 15 cel left and right.Continue to cultivate 6 days, the utilization ratio of steel cylinder carbonic acid gas is 75%.
Claims (15)
1. a horizontal submergence bell-type carbon compensator of cultivating micro-algae for open pond, comprise 1 or 2 above cover (1) and gas distributors (2), it is characterized in that, described gas distributor (2) is arranged at bottom, open pond, described cover (1) is the cylindrical shell of both ends open, along nutrient solution flow direction, arrange, the two-port of cover (1) is respectively nutrient solution import (3) and nutrient solution outlet (4); At the bottom of described cover (1) is directly fixed on open pond or built on stilts be arranged at open pond at the bottom of; Wherein, described cover (1) bottom is open, covers in gas distributor (2) top; Or be enclosed construction bottom described cover (1), described gas distributor (2) is positioned at the inside of cover (1); Described gas distributor (2) exports (4) distance more than 40 centimetres with the nutrient solution of cover (1).
2. carbon compensator according to claim 1, is characterized in that, described gas distributor (2) exports 60～750 centimetres of (4) distances with the nutrient solution of cover (1).
3. carbon compensator according to claim 1, is characterized in that, main body semicircular in shape cylindrical shell, arc cylindrical shell, rectangle cylindrical shell or the trapezoidal cylindrical shell of described cover (1).
4. carbon compensator according to claim 1, is characterized in that, the two-port of described cover (1) is taper or flaring flaring structure.
5. carbon compensator according to claim 1, is characterized in that, described cover (1) is highly 2～20 centimetres.
6. carbon compensator according to claim 1, is characterized in that, described cover (1) width is more than or equal to 2 centimetres.
7. carbon compensator according to claim 1, is characterized in that, described 2 above covers (1) form conjuncted structure.
8. the benefit carbon method of cultivating the horizontal submergence bell-type carbon compensator of micro-algae for open pond based on claim 1, it is characterized in that, horizontal submergence bell-type carbon compensator is arranged to bottom, open pond along open pond runner direction, make nutrient solution submergence cross cover top, nutrient solution nutrient solution import (3) from cover (1) under the promotion of the open original agitator in pond (9) flows into, the CO emitting with gas distributor in carbon compensator
2after contact, from nutrient solution outlet (4), flow out;
Nutrient solution flow velocity in described open pond is 20～50 cels;
CO in described horizontal submergence bell-type carbon compensator
2flow, amounts to the pure CO of the normal conditions
2, be 0.1～20 liter/min of every meter of open pond width.
9. benefit carbon method according to claim 8, is characterized in that, in described open pond, medium depth is 2～30 centimetres.
10. benefit carbon method according to claim 8, is characterized in that, described agitator blade tip is lower than at the bottom of opening pond.
11. benefit carbon methods according to claim 8, is characterized in that, described horizontal submergence bell-type carbon compensator is arranged in the shallow slot of bottom, open pond.
12. benefit carbon methods according to claim 11, is characterized in that, described shallow slot bottom is for flat or with the flat or Semi round bottom of fillet.
13. benefit carbon methods according to claim 8, is characterized in that described CO
2for purifying flue gas, industrial CO
2gas, pure CO
2gas or be mixed with CO
2airborne one or more or liquid CO
2.
14. benefit carbon methods according to claim 8, is characterized in that, the substratum of the micro-algae of described cultivation is Zarrouk substratum, SM substratum, ASP
2substratum or BG-11 substratum.
15. benefit carbon methods according to claim 8, is characterized in that, described micro-algae is spirulina, grid algae, Haematocoocus Pluvialls, salt algae, chlorella or chlamydomonas.
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| CN105002086B (en) * | 2015-07-16 | 2017-12-01 | 中国海洋大学 | A kind of raceway pond microalgae cultivating system for continuing aerating collecting frustule using microbubble |
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| CN101463321A (en) * | 2009-01-07 | 2009-06-24 | 福清市新大泽螺旋藻有限公司 | Gas distribution method and apparatus for carbon dioxide of big pool cultivated Spirulina |
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| Title |
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| 汪芳明 等.螺旋藻生产中两种碳营养源的对比研究.《江苏科技大学学报》.2006,第20卷(第1期),89-92. |
| 螺旋藻生产中两种碳营养源的对比研究;汪芳明 等;《江苏科技大学学报》;20060228;第20卷(第1期);第90页1.1实验材料，1.2实验内容和方法，图1 * |
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