CN103627632A - Photobioreactor for raceway pond - Google Patents
Photobioreactor for raceway pond Download PDFInfo
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- CN103627632A CN103627632A CN201310609278.4A CN201310609278A CN103627632A CN 103627632 A CN103627632 A CN 103627632A CN 201310609278 A CN201310609278 A CN 201310609278A CN 103627632 A CN103627632 A CN 103627632A
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- raceway pond
- carbonic acid
- pond
- raceway
- acid gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/18—Open ponds; Greenhouse type or underground installations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
Abstract
The invention provides a photobioreactor for a raceway pond. The photobioreactor comprises the raceway pond (1), a variable-speed stirring system, a carbon dioxide supply system (4) and a ground temperature control system. Compared with the prior art, the photobioreactor has the advantages that firstly, a variable hydraulic stirring system is introduced into the reactor, so as to be suitable for cultivation of microalgae with different mechanical resistance types; secondly, compared with the traditional motor, one hydraulic stirring system can drive multiple raceway ponds to realize large-scale application, so that the investment cost and the operating energy consumption can be greatly reduced; thirdly, the reactor is additionally provided with the carbon dioxide supply system and a gas refining device is arranged at the tail end of the carbon dioxide supply system, therefore, the detention time of carbon dioxide in water is prolonged and the utilization rate of the carbon dioxide by microalgae is improved; fourthly, a plastic film is arranged at the top of the raceway pond, so that the external pollution probability is reduced; and fifthly, the novel cyclic temperature control system is adopted, so that the cultivation time of the microalgae in winter and summer is prolonged and the energy consumption of the reactor is reduced.
Description
Technical field
The present invention relates to micro-algae culturing engineering field, particularly a kind of raceway pond bioreactor.
Background technology
Micro-algae is the main producer of natural water, plays a significant role, every year by the fixing CO of micro-algae photosynthesis in global Conversion of energy and Carbon cycle
2account for global CO
2the more than 40% of fixed amount.Meanwhile, the high added value biologically active substances such as microalgae cell rich in proteins, pigment and multiple unsaturated fatty acids, the important materials that progressively becomes the fields such as protective foods, medicine and fine chemistry industry is originated.Along with the exhaustion day by day of traditional fossil energy (oil, coal etc.), micro-algae has obtained people's great attention as renewable energy source (grease etc.), and micro-algae industry with important society and environmental effect demonstrates wide application prospect.
At present, the mass-producing of micro-algae is cultivated and is mainly contained open and closed two kinds of training modes.The reactor of closed cultivation mainly contains pillar, board-like and tubular type bioreactor, its advantage be the efficiency of light energy utilization high, be difficult for to pollute, culture density is high, but the shortcoming such as the investment of closed cultivation and running cost are higher, tonne amplification is unstable, cleaning and sterilizing is more difficult.
The representative reactions device of open cultivation is racetrack cultivation pool, as exploitation the earliest with most widely used training method, have build simple, cost of investment is low, tonne amplify the advantages such as simple, cleaning and sterilizing is convenient.Be widely used in Wei Zao both domestic and external manufacturing enterprise: U.S. Cyanotech company, New Zealand Aquaflow Bionomic company, Israel Seambiotc company, Britain AlgaeVS company; Century-old nutritive food company limited, Jing Zhou astaxanthin company limited, Yunnan Lin Long both culturing microalgae company limited etc. are grant in domestic Yunnan green A biotechnology company limited, Jiangsu.But raceway pond equally also exists, the efficiency of light energy utilization is low, the shortcoming of easy pollution.Especially the micro-algae to some universality growth conditionss, is allowed to condition at Initial stage of culture and adapts to sooner raceway pond habitat, becomes rapidly sociales, will greatly improve the industrialized success ratio of micro-algae.
[0005] circulation stirring system is the core component of raceway pond, its Main Function has: one, ensure stirring of nutritive salt in nutrient solution, make frustule can obtain sufficient nutrient supply in each position of nutrient solution, two, ensure sufficient gaseous interchange, make atmosphere or special-purpose CO
2the CO of gas tank
2in algae liquid, fully dissolve to ensure and photosyntheticly carry out smoothly, three, guarantee that algae liquid flows at cultivation pool internal recycle, ensureing that micro-algae is evenly distributed in nutrient solution improves its efficiency of light energy utilization, avoids the gathering sedimentation of frustule.
Current raceway pond reactor adopts driven by motor paddle wheel to stir more, and in order to reach lower rotating speed, the common spindle wheel swinging reducer that mostly is.Motor stirring system is mostly constant speed and stirs, although and buncher can reach the object of output rotating speed regulation, cost is too high on the one hand, the slow speed of revolution that cannot meet on the other hand algae culture requires (5-50 rev/min).
The major defect of motor constant-speed forward stirring system in micro-algae is cultivated has: (1) cannot solve the contradiction between the different growth phases of micro-algae, growth conditions and corresponding stirring frequency.For example, at the early growth period of micro-algae, need lower disturbance and flow rate, there is restraining effect to the micro-algae of part in high stirring frequency more; At later stages, for ensureing better gaseous interchange and avoiding the gathering of high-density frustule to sink to needing higher stirring frequency; (2) be applicable to the algae strain that mechanical resistance is stronger more cultivate (chlorella, spirulina etc.), for mechanical resistance, weak algae strain (Haematocoocus Pluvialls etc.) is cultivated and is had restriction; (3) in motor operation course, energy consumption is higher.
Shortcoming for circulation stirring system, " a kind of production equipment of industrialized microalgae cultivation " (Chinese patent of Cai Zhiwu, CN201245640Y) a kind of production equipment of realizing the circulation of algae liquid by air-blowing mode is disclosed, the shortcoming of this device is that pipeline is complicated, be not suitable for extensive amplification, be not suitable for all algae strains and cultivate." a kind of raceway pond bioreactor of realizing micro-algae large-scale cultivation " (Chinese patent, the CN102304462 B) of Zhang Chengwu etc. discloses two cover algae fluid circulations (being respectively the stirring rake recycle system and the air-blowing air lift type recycle system) and cultivated to adapt to the algae strain of different resistances.The shortcoming of this device is complicacy and the cost of investment that has increased raceway pond structure, has also strengthened the operation expense in later stage simultaneously, is unfavorable for cleaning and sterilizing.
Meanwhile, also there is how low-cost effectively temperature control, CO in traditional raceway pond bioreactor
2the shortcomings such as utilization ratio is on the low side.
Therefore, urgently design a kind of novel raceway pond bioreactor scheme to solve the above-mentioned defect of existing raceway pond bioreactor.
Summary of the invention
Of the present invention being at present for the problem existing in background technology, provides a kind of raceway pond bioreactor, in order to realize the low-cost high-density of micro-algae, cultivates.
For solving above technical problem, technical scheme provided by the invention is,
A raceway pond bioreactor, comprises raceway pond (1), speed change stirring system, carbonic acid gas make up system (4), ground temperature controlling system,
Described speed change stirring system comprises hydraulic power system (2) and stirring rake (3), and described stirring rake is placed in raceway pond, and algae liquid is flowed at raceway pond internal recycle;
Hydraulic power system (2) comprising: for stirring rake is inputted the oil motor of variable propulsion source and driven motor (31), oil pump (32) and adjusting, the control rotating speed of oil motor and the pressure controlled valve of direction (34), flowrate control valve (36), the direction control valve (35) of oil motor to form;
Described carbonic acid gas make up system (4) comprises gas bomb, carbonic acid gas deep trouth and gas atomizer, and at the bottom of carbonic acid gas deep trouth is placed in raceway pond (1) pond, described gas atomizer is arranged in carbonic acid gas deep trouth;
Described ground temperature controlling system comprises underground aqua storage tank (51), temperature controlling system (52) and loop temperature-control pipeline (54), and being positioned at valve and the water pump on described loop temperature-control pipeline, described loop temperature-control pipeline is formed side by side by the branch line that is distributed in raceway pond (1).
As preference:
Rollable plastic, transparent film is installed directly over described raceway pond.
Compared with prior art, the present invention has the following advantages: (1) introduces reactor design by variable hydraulic pressure stirring system, thereby is applicable to micro-algae cultivation of different mechanical resistance types; (2) use motor comparison with tradition, a hydraulic pressure stirring system can drive a plurality of raceway ponds, and mass-producing application can reduce cost of investment and operation energy consumption greatly; (3) reactor increases carbonic acid gas make up system, and make up system end arranges gas atomizer, extends the residence time of carbonic acid gas in water, improves the utilization ratio of micro-algae to carbonic acid gas; (4) raceway pond top arranges plastics film, lowers external pollution probability; (5) adopt novel loop temperature-control system, extend micro-algae in the winter time with the incubation time in summer, reduce reactor energy consumption simultaneously.
Accompanying drawing explanation
Fig. 1 is raceway pond bioreactor structural representation;
Fig. 2 is the structural representation of hydraulic circulation stirring system.
Fig. 3 is the structural representation of ground temperature controlling system.
1--raceway pond; 2--hydraulic power system; 3--stirring rake; 4--carbonic acid gas make up system; 31--motor; 32--oil pump; 33--fuel tank; 34--pressure controlled valve; 35--direction control valve; 36--flowrate control valve; 37--oil motor forms; 51--underground aqua storage tank; 52--temperature controlling system; 54--loop temperature-control pipeline.
Embodiment
The present invention is further illustrated by following unrestriced embodiment, but non-in order to limit the scope of the invention.
?
1. as Fig. 1, shown in 2, Fig. 3, a kind of raceway pond bioreactor, comprises raceway pond (1), speed change stirring system, carbonic acid gas make up system (4), ground temperature controlling system,
Described speed change stirring system comprises hydraulic power system (2) and stirring rake (3), and described stirring rake is placed in raceway pond, and algae liquid is flowed at raceway pond internal recycle;
Hydraulic power system (2) comprising: for stirring rake is inputted the oil motor of variable propulsion source and driven motor (31), oil pump (32) and adjusting, the control rotating speed of oil motor and the pressure controlled valve of direction (34), flowrate control valve (36), the direction control valve (35) of oil motor to form;
Described carbonic acid gas make up system (4) comprises gas bomb, carbonic acid gas deep trouth and gas atomizer, and at the bottom of carbonic acid gas deep trouth is placed in raceway pond (1) pond, described gas atomizer is arranged in carbonic acid gas deep trouth;
Described ground temperature controlling system comprises underground aqua storage tank (51), temperature controlling system (52) and loop temperature-control pipeline (54), and being positioned at valve and the water pump on described loop temperature-control pipeline, described loop temperature-control pipeline is formed side by side by the branch line that is distributed in raceway pond (1).
Rollable plastic, transparent film is installed directly over raceway pond.
The shallow pond of ellipse that described raceway pond 1 is built into by cement, is highly generally 10-50cm, and length and width are without particular requirement, and raceway pond internal surface is smooth, can overlay the easy cleaning lubricious material such as PVC leather, plastics film in pond.Hydraulic efficiency system, for raceway pond algae liquid provides power, by hydraulic power being transferred to oil motor 37, through hydraulic motor transmission stirring rake 2, is reached for the object that the circulation of raceway pond algae liquid provides power.Torsion and the rotating speed adjustable (>=5 revs/min) of stirring rake, the difference to water power demand according to the difference of different algae strain growth conditionss and the different growth phases of algae strain, regulate pressure controlled valve and flowrate control valve to control torsion and the rotating speed of stirring rake, mechanical resistance algae strain strong and that mechanical resistance is weak is all applicable.Stirring rake need adopt corrosion resistant material to make.
Described carbonic acid gas make up system 4 is mainly used in the supply of carbonic acid gas in nutrient solution,
Described ground temperature controlling system is mainly used in the intensification in nutrient solution winter and the cooling in summer, described underground conservation pool is arranged in underground, stable (cool in summer and warm in winter) that is conducive to water temperature, reduce energy consumption, can use the exhaust-heat heating of well heater or boiler simultaneously winter, can use summer cooling-water machine to help cooling, ensure the water temperature that is suitable for algae strain growth.Described circulation line is layed in raceway pond.
For the present invention is described better, be convenient to understand technical scheme of the present invention, carried out following simultaneous test:
Simultaneous test one
Two long be 4 meters, wide be 2 meter Tuo garden type raceway ponds carry out Haematocoocus Pluvialls in sunlight booth (
haematococcus) cultivate.Nutrient solution height is 0.15 meter (1200 liters of liquid amounts), adopts and adds rich MCM substratum, cell inoculum density 2.0*10
4individual/mL.The stirring system that wherein contrasts raceway pond drives by motor, and whole culture cycle medium speed is 30 revs/min, without carbonic acid gas, supplements system.Experimental group adopts this experiment Novel running track pond, at the algae liquid access initial stage, stirring frequency is 10 revs/min, at algae liquid, cultivates mid-term, is adjusted to 17 revs/min on rotating speed, cultivate latter stage to algae liquid, 30 revs/min of rotating-speed modulations, carbon dioxide supplement system in culturing process, passes into mixed gas (5% CO2 of air/CO2, v/v), air flow is 0.2VVM.In culture cycle, temperature on average and intensity of illumination are: during 8:00 17 ℃, and 150umol/m
2/ s, during 13:00 29 ℃, 400umol/m
2/ s, during 17:00 23 ℃, 170umol/m
2/ s.Cultivate after 10 days, sampling analysis, frustule concentration reaches 1.9*10
5individual/mL, and control group to cultivate frustule concentration after 10 days be 1.1 * 10
5individual/mL.With respect to control group, cell cultures concentration has improved 72.7%.
Simultaneous test two
Two long be 4 meters, wide be 2 meter Tuo garden type raceway ponds carry out Microcystis aeruginosa in sunlight booth (
microcystis) cultivate.Nutrient solution height is 0.15 meter (1200 liters of liquid amounts), adopts MA substratum, cell inoculum density 1.2*10
6individual/mL.The stirring system that wherein contrasts raceway pond drives by motor, and whole culture cycle medium speed is 30 revs/min, without carbonic acid gas, supplements system.Experimental group adopts this experiment Novel running track pond, at the algae liquid access initial stage, stirring frequency is 15 revs/min, at algae liquid, cultivates mid-term, is adjusted to 25 revs/min on rotating speed, cultivate latter stage to algae liquid, 33 revs/min of rotating-speed modulations, carbon dioxide supplement system in culturing process, passes into mixed gas (5% CO2 of air/CO2, v/v), air flow is 0.2VVM.In culture cycle, temperature on average and intensity of illumination are: during 8:00 24 ℃, and 190umol/m
2/ s, during 13:00 36 ℃, 630umol/m
2/ s, during 17:00 30 ℃, 230umol/m
2/ s.Cultivate after 10 days, sampling analysis, frustule concentration reaches 4.7*10
7individual/mL, and control group to cultivate frustule concentration after 10 days be 2.6 * 10
7individual/mL.With respect to control group, cell cultures concentration has improved 80.8%.
Simultaneous test three
Raceway pond cooling system is set up, cooling-water machine (wet curtain and blower fan) is lowered the temperature to the water of underground conservation pool, cooling circulating water flows out through underground conservation pool, after water pump pressurization, enter branch's water pipe, each raceway pond arranges two groups of water pipes, and recirculated water finally flows back to underground conservation pool, stablizes the water temperature of nutrient solution by recirculated water, ensure the normal culture temperature of micro-algae, the Extending culture cycle.
Applicant's statement; the present invention illustrates that by above-described embodiment structure of the present invention forms; but the present invention is not limited to said structure; person of ordinary skill in the field should understand; any improvement in the present invention; to the selection of the interpolation of the equivalence replacement of the selected parts of the present invention and accessory, concrete mode etc., within all dropping on protection scope of the present invention and open scope.
Claims (2)
1. a raceway pond bioreactor, is characterized in that: comprise raceway pond (1), speed change stirring system, carbonic acid gas make up system (4), and ground temperature controlling system,
Described speed change stirring system comprises hydraulic power system (2) and stirring rake (3), and described stirring rake is placed in raceway pond, and algae liquid is flowed at raceway pond internal recycle;
Hydraulic power system (2) comprising: for stirring rake is inputted the oil motor of variable propulsion source and driven motor (31), oil pump (32) and adjusting, the control rotating speed of oil motor and the pressure controlled valve of direction (34), flowrate control valve (36), the direction control valve (35) of oil motor to form;
Described carbonic acid gas make up system (4) comprises gas bomb, carbonic acid gas deep trouth and gas atomizer, and at the bottom of carbonic acid gas deep trouth is placed in raceway pond (1) pond, described gas atomizer is arranged in carbonic acid gas deep trouth;
Described ground temperature controlling system comprises underground aqua storage tank (51), temperature controlling system (52) and loop temperature-control pipeline (54), and being positioned at valve and the water pump on described loop temperature-control pipeline, described loop temperature-control pipeline is formed side by side by the branch line that is distributed in raceway pond (1).
2. the raceway pond bioreactor as described in claim 1, is characterized in that, rollable plastic, transparent film is installed directly over described raceway pond.
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| CN201310609278.4A CN103627632A (en) | 2013-11-27 | 2013-11-27 | Photobioreactor for raceway pond |
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| CN201310609278.4A CN103627632A (en) | 2013-11-27 | 2013-11-27 | Photobioreactor for raceway pond |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726321A (en) * | 2015-04-15 | 2015-06-24 | 中国海洋大学 | Runway type bioreactor applicable to sunshine factorization |
| CN104898725A (en) * | 2015-06-18 | 2015-09-09 | 国家海洋局第三海洋研究所 | Seawater acidification control system for simulating future ocean acidification |
| GB2539936A (en) * | 2015-07-01 | 2017-01-04 | Univ Nelson Mandela Metropolitan | Microalgae cultivation process and equipment |
| KR20210007132A (en) * | 2019-07-10 | 2021-01-20 | 강원도 삼척시(농업기술센터장) | Continuos incubating apparatus for microalgae capable of selective controlling of cultivation environment |
| IT202100025295A1 (en) * | 2021-10-01 | 2023-04-01 | Graziano Forni | PLANT FOR THE PRODUCTION OF MICROALGAE |
| CN116621343A (en) * | 2023-05-26 | 2023-08-22 | 武汉科技大学 | Photobioreactor for advanced treatment of domestic sewage by adopting chlorella hupezii particles |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726321A (en) * | 2015-04-15 | 2015-06-24 | 中国海洋大学 | Runway type bioreactor applicable to sunshine factorization |
| CN104726321B (en) * | 2015-04-15 | 2017-06-06 | 中国海洋大学 | A kind of racetrack bioreactor suitable for sunlight batch production |
| CN104898725A (en) * | 2015-06-18 | 2015-09-09 | 国家海洋局第三海洋研究所 | Seawater acidification control system for simulating future ocean acidification |
| GB2539936A (en) * | 2015-07-01 | 2017-01-04 | Univ Nelson Mandela Metropolitan | Microalgae cultivation process and equipment |
| AU2016285489B2 (en) * | 2015-07-01 | 2021-05-20 | Nelson Mandela University | Microalgae production process and equipment |
| KR20210007132A (en) * | 2019-07-10 | 2021-01-20 | 강원도 삼척시(농업기술센터장) | Continuos incubating apparatus for microalgae capable of selective controlling of cultivation environment |
| KR102273724B1 (en) | 2019-07-10 | 2021-07-06 | 강원도 삼척시(농업기술센터장) | Continuos incubating apparatus for microalgae capable of selective controlling of cultivation environment |
| IT202100025295A1 (en) * | 2021-10-01 | 2023-04-01 | Graziano Forni | PLANT FOR THE PRODUCTION OF MICROALGAE |
| CN116621343A (en) * | 2023-05-26 | 2023-08-22 | 武汉科技大学 | Photobioreactor for advanced treatment of domestic sewage by adopting chlorella hupezii particles |
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Application publication date: 20140312 |