CN105695313B - A kind of positive conveyance tubular type bioreactor - Google Patents

A kind of positive conveyance tubular type bioreactor Download PDF

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Publication number
CN105695313B
CN105695313B CN201610273636.2A CN201610273636A CN105695313B CN 105695313 B CN105695313 B CN 105695313B CN 201610273636 A CN201610273636 A CN 201610273636A CN 105695313 B CN105695313 B CN 105695313B
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sealing
plug
photosynthetic
gear
tubular type
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CN105695313A (en
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杨卫民
王晗
关昌峰
阎华�
何立臣
丁玉梅
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Beijing University of Chemical Technology
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Beijing University of Chemical Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention proposes a kind of positive conveyance tubular type bioreactor, is made up of positive displacement transmission system, photosynthetic reaction system and the part of Holder Fasteners three.Positive displacement transmission system is made up of gear, power transmission shaft, motor, sealing-plug, chain, approach cone and upper hose, rotated using motor driven gear, by chain drive, make sealing-plug that algae solution segmented seal are carried into photosynthetic reaction system in upper hose, power resources are provided for algae solution circulation；Photosynthetic reaction system includes peace in photosynthetic response pipeline section, bypass channel, tank, connecting tube and outlet pipe, photosynthetic response pipeline section and turns unit combined rotor, and reacting environment is provided for microalgae photosynthetic response；Holder Fasteners include motor rack, gear supporting plate and support frame, and whole system is supported and fixed.Injured present invention, avoiding microalgae cell by pump shearing force, beneficial to realizing microalgae High Density Cultivation；The photosynthetic response pipeline section number that bypass channel is connected can be adjusted according to flow, flow velocity and culture scale, flexible structure, strong adaptability.

Description

A kind of positive conveyance tubular type bioreactor
Technical field
The present invention relates to a kind of pipe for the bioreactor, more particularly to positive conveyance manually cultivated for microalgae Formula bioreactor, belongs to technical field of microalga biology.
Background technology
Microalgae is photosynthetic efficiency highest primordial plant, and the productivity per unit than crops is higher by decades of times, is nature Boundary grows most fast plant.The especially unicellular microalgae of algae can effectively utilize luminous energy, carbon dioxide and inorganic salts synthesis egg White matter, grease, carbohydrate and a variety of high added value bioactive substances.But the microalgae that nature is directly obtained is not The extraction of high value added product can be used for, it is still necessary to which artificial large-scale culture to obtain raw materials for production.The High Density Cultivation of microalgae is The key that microalgae resource synthetic development is utilized is realized, and the bioreactor that structure is suitable for micro algae growth is then to realize microalgae The important topic of High Density Cultivation.
Bioreactor generally refers to cultivate photosynthetic tiny organism and plant tissue with photosynthetic capacity, cell Facility or device, the generally regulation and control system with condition of culture such as light, temperature, pH value, nutritive salt, gas exchanges, can enter Capable semicontinuous or continuous culture simultaneously has the higher efficiency of light energy utilization, thus can obtain higher biological density and unit plane (body) Product yield.At present, bioreactor mainly has Race-way photobioreactor and the major class of closed photo bioreactor two, opens The structure of putting formula bioreactor is relatively easy, manufacturing price is low, be easily achieved, but open devices have it is very many not Foot, it is only used for the culture of the algae kind of a small number of strong stress resistances, and there is easy dye miscellaneous bacteria simultaneously, is difficult to control to culture ginseng Number, water evaporation capacity is big, the problems such as condition of culture is unstable, thus many scholars think Race-way photobioreactor The space not continued to lift up, so that development sight has been turned into closed photo bioreactor.With open optical-biological reaction Device is compared, and closed photo bioreactor production period is longer, and various parameters control accuracy is higher, and algae solution concentration is higher.Seal at present The polytypes such as enclosed reactor existing tubular type, board-like, gas-lifting type, pillar.Compared with other types bioreactor, tubular type Bioreactor have that illumination specific surface area is big, culture parameters are easy to monitoring and can the advantage such as continuous production, turn into Obtain the bioreactor type of large-scale application.
But tubular type bioreactor there is also mixing mass transfer effect it is poor, along pipe range direction dissolved oxygen easily accumulation and Microalgae is easily grown nonparasitically upon another plant in subject matters such as reactor walls, have impact on its application effect, especially because tubular type optical-biological reaction The general diameter of device is smaller, and cleaning is difficult, and attached wall phenomenon can have a strong impact on production efficiency and the microalgae life of tubular type bioreactor The raising of long density.For problem above, Chinese utility model patent ZL201520040553.X proposes a kind of intelligent dimming certainly Tubular type photosynthetic organism reaction system is cleaned, drives combined rotor in photosynthetic reactor to rotate using flow of fluid, microalgae, which is followed, to be turned Sub inside and outside rolling, produces flow-disturbing effect, and radial second stream of the increase mixing micro algae culturing liquid in reactor is conducive to nutrient to pass Matter is disperseed, enhanced heat exchange.Unit combined rotor is obviously promoted effect to microdisk electrode density, but must adopt within the system The circulation of algae solution is realized with pump, if selection centrifugal pump, axial-flow pump etc. can all produce shearing injury to microalgae cell, selection is wriggled Pump does not have shearing force, but flow is too small, it is impossible to applied to micro-algae large-scale culture.Thus the selection of pump seriously governs intelligence The performance of automatically cleaning tubular type photosynthetic organism reaction system can be dimmed.
The content of the invention
To solve the above problems, the technical solution adopted by the present invention is as follows：A kind of positive conveyance tubular type optical-biological reaction Device, is made up of positive displacement transmission system, photosynthetic reaction system and the part of Holder Fasteners three, and positive displacement transmission system is by gear, biography Moving axis, motor, sealing-plug, chain, approach cone and upper hose composition, are rotated using motor driven gear, by chain drive, made Algae solution segmented seal is carried into photosynthetic reaction system by sealing-plug in upper hose, and power resources are provided for algae solution circulation；Light Closing reaction system includes photosynthetic response pipeline section, bypass channel, tank, connecting tube, outlet pipe etc., and the interior peace of photosynthetic response pipeline section turns unit Combined rotor, reacting environment is provided for microalgae photosynthetic response；Holder Fasteners include motor rack, gear supporting plate and support frame etc., Whole system is supported and fixed.
Motor drives driving gear to rotate by power transmission shaft, under the drive of chain, and driven gear turns around rotary gear shaft Dynamic, chain is fixed on installation, chain through pin hole by pin by inner plate and outer plate and is uniformly provided with sealing-plug, It is fixed between sealing-plug main body and chain by voussoir；Sealing ring can be directly sleeved in sealing-plug main body, will can also be sealed Plug main body is processed as the shape of middle trough of belt, it is to avoid sealing ring slides axially along chain, is ground when using a period of time sealing ring When damage causes poorly sealed, it can pull down and new sealing ring is installed.With the motion of chain, sealing-plug enters upper water by approach cone Pipe, and moved from bottom to top in upper hose, sealing ring and the upper hose tube wall of sealing-plug main body outer ring are fitted close, and upper water Polytetrafluorethylecoatings coatings are scribbled in pipe to reduce the algae solution in abrasion, the increase pressure reduction of sealing-plug lower tubular section volume, tank Follow sealing-plug to move and enter upper hose, photosynthetic reaction system is entered by outlet pipe after reaching certain altitude.On chain The even spacing for installing sealing-plug should be less than upper hose length, it is ensured that moves and is produced enough in space above and below it upwards with sealing-plug Pressure difference, upper hose is sucked by algae solution, is realized the positive conveyance of algae solution, is circulated in the entire system.
Algae solution enters after photosynthetic reaction system, enters bypass channel by outlet pipe first, bypass channel can be according to flow velocity, flow And be connected the need for production scale etc. with many photosynthetic response pipeline sections, bypass channel is connected with photosynthetic response pipeline section by connecting tube, Connected mode can be using the mode such as threaded connection or flange connection.Photosynthetic response pipeline section is built with unit combined rotor, including limit Position part, suspension member, rotor and rotating shaft, algae solution flowing rotor driven are rotated, so as to produce flow-disturbing, make nutritional ingredient, carbon dioxide, micro- Frustule etc. is all more uniformly distributed.Algae solution fully carries out photosynthesis after photosynthetic response pipeline section, flows back to tank, its reclaimed water Algae solution highly should be higher than that plane where approach cone arrival end in groove.
Holder Fasteners provide a supporting role for whole device.Motor rack is used to place motor, makes motor output end and active Gear centre is connected in same level height by power transmission shaft.Support frame, left crossbeam constitute photosynthetic reaction system with right crossbeam Support section, bypass channel is ridden on support frame, and right crossbeam is provided with semicircle orifice, the fixation for photosynthetic response pipeline section.Driving gear The rotary gear shaft of power transmission shaft and the driven gear connection of connection is all connected in gear supporting plate, and gear supporting plate is welded with left crossbeam It is connected together, gear position is fixed.
The illumination of the bioreactor is shone using exterior light, and water bath containment can get up as needed, is formed closed Culture environment, carbon dioxide, plus sample tap etc. are led into tank.
A kind of positive conveyance tubular type bioreactor of the present invention, its advantage and act as：
(1) gear drive under motor drives realizes positive conveyance of the sealing-plug to algae solution in upper hose, makes algae Liquid is in photosynthetic reaction system internal circulation flow, and positive conveyance mode flow is big, and algae solution flowing can drive unit combined rotor Rotate, present invention, avoiding shearing force injury of the microalgae cell by pump, beneficial to the High Density Cultivation for realizing microalgae；
(2) the photosynthetic response pipeline section number that bypass channel is connected can be adjusted according to flow, flow velocity and culture scale, structure spirit It is living, strong adaptability；
(3) training mode of dynamic circulation substantially increases the growth rate of microalgae, is trained beneficial to the scale for realizing microalgae Support.
Brief description of the drawings
Fig. 1 is a kind of structure chart of positive conveyance tubular type bioreactor of the invention.
Fig. 2 is Fig. 1 top view.
Fig. 3 is Fig. 1 right view.
Fig. 4 is chain and structure chart at sealing-plug cooperation.
Fig. 5 is Fig. 4 left view.
Fig. 6 is Fig. 4 top view.
Fig. 7 is chain and structure chart at sealing-plug cooperation in groove.
Fig. 8 is photosynthetic response pipe segment structure figure.
In figure：1- driven gears, 2- rotary gear shafts, 3- approach cones, the left crossbeams of 4-, 5- gear supporting plates, 6- upper hoses, 7- Motor rack, 8- motors, 9- sealing-plugs, 10- chains, 11- driving gears, 12- power transmission shafts, 13- outlet pipes, 14- bypass channels, 15- Outside connecting tube, 16- support frames, the right crossbeams of 17-, 18- photosynthetic response pipeline sections, 19- tanks, 20- inner plates, 21- pin holes, 22- Carrier bar, 23- pins, 24- sealing-plug main bodys, 25- sealing rings, 26- voussoirs, 27- locating parts, 28- suspension members, 29- rotors, 30- turns Axle.
Embodiment
As shown in Figure 1, Figure 2, Figure 3 shows, a kind of positive conveyance tubular type bioreactor of the invention, by positive displacement power train System, photosynthetic reaction system and the part of Holder Fasteners three composition, positive displacement transmission system is by gear, power transmission shaft 12, motor 8, sealing Plug 9, chain 10, approach cone 3 and upper hose 6 are constituted, and are driven pinion rotation using motor 8, are driven by chain 10, make sealing-plug Algae solution segmented seal is carried into photosynthetic reaction system by 9 in upper hose 6, and power resources are provided for algae solution circulation；It is photosynthetic anti- System is answered to include photosynthetic response pipeline section 18, bypass channel 14, tank 19, connecting tube 15 and outlet pipe 13 etc., photosynthetic response pipeline section 18 Interior peace turns unit combined rotor, and reacting environment is provided for microalgae photosynthetic response；Holder Fasteners include motor rack 7, gear supporting plate 5 With support frame 16 etc., whole system is supported and fixed.
As Figure 4-Figure 7, motor 8 drives driving gear 11 to rotate by power transmission shaft 12, under the drive of chain 10, from Moving gear 1 is rotated around rotary gear shaft 2, and chain 10 is carried out by inner plate 20 and outer plate 22 by pin 23 through pin hole 21 It is fixedly mounted, sealing-plug 9 uniformly is installed on chain 10, consolidated between sealing-plug main body 24 and chain 10 by voussoir 26； Sealing ring 25 can be directly sleeved in sealing-plug main body 24, and sealing-plug main body 24 can be also processed as to the shape of middle trough of belt, it is to avoid Sealing ring 25 slides axially along chain 10, when causing poorly sealed when being worn using a period of time sealing ring 25, can pull down peace Fill new sealing ring 25.With the motion of chain 10, sealing-plug 9 enters upper hose 6 by approach cone 3, and in upper hose 6 under And upper motion, sealing ring 25 and the tube wall of upper hose 6 of the outer ring of sealing-plug main body 24 are fitted close, and poly- four are scribbled in upper hose 6 PVF coating follows sealing-plug to reduce the algae solution in abrasion, the increase pressure reduction of the lower tubular section volume of sealing-plug 9, tank 19 9 move and enter upper hose 6, enter photosynthetic reaction system by outlet pipe 13 after reaching certain altitude.Uniformly pacify on chain 10 The spacing for filling sealing-plug 9 should be less than the length of upper hose 6, it is ensured that moves, is produced enough in space above and below it upwards with sealing-plug 9 Pressure difference, realizes the positive conveyance of algae solution, circulates in the entire system.
As shown in figure 8, algae solution enters after photosynthetic reaction system, bypass channel 14, bypass channel are entered by outlet pipe 13 first 14 can according to being connected with many photosynthetic response pipeline sections 18 the need for flow velocity, flow and production scale etc., bypass channel 14 with it is photosynthetic Reaction pipeline section 18 is connected by connecting tube 15, and connected mode can be using the mode such as threaded connection or flange connection.Photosynthetic response pipeline section 18 built with unit combined rotor, including locating part 27, suspension member 28, rotor 29 and rotating shaft 30, algae solution 29 turns of rotor driven of flowing It is dynamic, so as to produce flow-disturbing, nutritional ingredient, carbon dioxide, microalgae cell etc. is all more uniformly distributed.Algae solution passes through photosynthetic response Photosynthesis is fully carried out after pipeline section 18, tank 19 is flowed back to, algae solution highly should be higher than that the arrival end institute of approach cone 3 wherein in tank 19 In plane.
Holder Fasteners provide a supporting role for whole device.Motor rack 7 is used to place motor 8, makes the output end of motor 8 and master The center of moving gear 11 is connected in same level height by power transmission shaft 12.Support frame 16, left crossbeam 4 constitute light with right crossbeam 17 The support section of reaction system is closed, bypass channel 14 is ridden on support frame 16, and right crossbeam 17 is provided with semicircle orifice, for photosynthetic response pipe The fixation of section 18.The rotary gear shaft 2 that the power transmission shaft 12 and driven gear 1 that driving gear 11 is connected are connected all is connected to gear support On plate 5, gear supporting plate 5 welds together with left crossbeam 4, and gear position is fixed.
The illumination of the bioreactor is shone using exterior light, as needed can be enclosed tank 19, forms closing Formula culture environment, carbon dioxide, plus sample tap etc. are led into tank 19.

Claims (10)

1. a kind of positive conveyance tubular type bioreactor, it is characterised in that：Mainly by positive displacement transmission system, photosynthetic response System and the part of Holder Fasteners three composition, positive displacement transmission system is by gear, power transmission shaft, motor, sealing-plug, chain, approach cone With upper hose composition, rotated using motor driven gear, by chain drive, sealing-plug is segmented algae solution in upper hose close Envelope is carried into photosynthetic reaction system, and power resources are provided for algae solution circulation；Photosynthetic reaction system includes photosynthetic response pipeline section, divided Peace turns unit combined rotor in tank, tank, connecting tube and outlet pipe, photosynthetic response pipeline section, is provided instead for microalgae photosynthetic response Answer place；Holder Fasteners include motor rack, gear supporting plate and support frame, and whole system is supported and fixed；Sealing-plug is by entering Prostomum enters upper hose, and is moved from bottom to top in upper hose, and the sealing ring and upper hose tube wall of sealing-plug main body outer ring are tight Algae solution in close fit, the increase pressure reduction of sealing-plug lower tubular section volume, tank follows sealing-plug to move and enter upper hose, Photosynthetic reaction system is entered by outlet pipe after reaching certain altitude.
2. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Motor is by passing Moving axis drives driving gear to rotate, under the drive of chain, and driven gear is rotated around rotary gear shaft, and chain is by inner plate and outside Carrier bar by pin through pin hole be fixed on installation, chain it is uniform sealing-plug is installed, sealing-plug main body and chain it Between be fixed by voussoir.
3. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Sealing ring is direct It is enclosed in sealing-plug main body, or sealing-plug main body is processed as to the shape of middle trough of belt, it is to avoid sealing ring slides axially along chain, When causing poorly sealed when being worn using a period of time sealing ring, pull down and new sealing ring is installed.
4. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Applied in upper hose There are polytetrafluorethylecoatings coatings to reduce abrasion.
5. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：It is uniform on chain The spacing for installing sealing-plug should be less than upper hose length, it is ensured that is moved upwards with sealing-plug and produces enough pressures in space above and below it Difference, upper hose is sucked by algae solution, is realized the positive conveyance of algae solution, is circulated in the entire system.
6. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Bypass channel and light Close reaction pipeline section to be connected by connecting tube, connected mode is using threaded connection or flange connection.
7. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Photosynthetic response pipe Section is built with unit combined rotor, including locating part, suspension member, rotor and rotating shaft, and algae solution flowing rotor driven is rotated, so as to produce Flow-disturbing, makes nutritional ingredient, carbon dioxide, microalgae cell all more be uniformly distributed.
8. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Algae solution passes through light Photosynthesis is fully carried out after closing reaction pipeline section, tank is flowed back to, algae solution highly should be higher than that approach cone arrival end institute wherein in tank In plane.
9. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Holder Fasteners are Whole device is provided a supporting role, and motor rack is used to place motor, makes motor output end with driving gear center in same level Highly, connected by power transmission shaft；Support frame, left crossbeam constitute the support section of photosynthetic reaction system with right crossbeam, and bypass channel is taken On support frame, right crossbeam is provided with semicircle orifice, the fixation for photosynthetic response pipeline section；The power transmission shaft and driven of driving gear connection The rotary gear shaft of gear connection is all connected in gear supporting plate, and gear supporting plate is together with left beam welding, by gear position Put fixation.
10. a kind of positive conveyance tubular type bioreactor according to claim 1, it is characterised in that：Illumination is used Exterior light is shone, and water bath containment is got up, and forms closed culture environment, and carbon dioxide is led into tank, increases sample tap.
CN201610273636.2A 2016-04-28 2016-04-28 A kind of positive conveyance tubular type bioreactor Active CN105695313B (en)

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Citations (2)

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Publication number Priority date Publication date Assignee Title
CN103289887A (en) * 2012-03-01 2013-09-11 中国科学院青岛生物能源与过程研究所 Half-dry solid-state adherent culture device for microalgae industrial production
CN104560702A (en) * 2015-01-21 2015-04-29 北京化工大学 Intelligent dimming self-cleaning pipe type photo-bioreactor

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US20140315291A1 (en) * 2013-04-18 2014-10-23 Superior Ecotech LLC Solar Conversion System And Methods
CN205590687U (en) * 2016-04-28 2016-09-21 北京化工大学 Normal position moves and carries tubular photobioreactor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103289887A (en) * 2012-03-01 2013-09-11 中国科学院青岛生物能源与过程研究所 Half-dry solid-state adherent culture device for microalgae industrial production
CN104560702A (en) * 2015-01-21 2015-04-29 北京化工大学 Intelligent dimming self-cleaning pipe type photo-bioreactor

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