CN220999635U - Microorganism constant temperature culture apparatus - Google Patents
Microorganism constant temperature culture apparatus Download PDFInfo
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- CN220999635U CN220999635U CN202322710153.2U CN202322710153U CN220999635U CN 220999635 U CN220999635 U CN 220999635U CN 202322710153 U CN202322710153 U CN 202322710153U CN 220999635 U CN220999635 U CN 220999635U
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- display control
- control screen
- microbial
- culture apparatus
- triangular flask
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- 244000005700 microbiome Species 0.000 title claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 38
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 16
- 239000001301 oxygen Substances 0.000 claims abstract description 16
- 238000001514 detection method Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 241001411320 Eriogonum inflatum Species 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- -1 polytetrafluoroethylene Polymers 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims 9
- 230000005611 electricity Effects 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 31
- 230000004151 fermentation Effects 0.000 abstract description 31
- 238000010008 shearing Methods 0.000 abstract description 8
- 241001052560 Thallis Species 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 6
- 238000009776 industrial production Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of microorganism culture and discloses a microorganism constant-temperature culture device, which comprises a box body, wherein a display control screen is arranged on the box body, and a plurality of groups of culture components are arranged in the box body; the culture assembly comprises a triangular flask, a stirring paddle is arranged in the triangular flask, the other end of the stirring paddle is connected with a motor, and the motor is electrically connected with the display control screen. According to the microorganism constant temperature culture device, the plurality of groups of culture components are arranged in the box body, and each group of culture components is independently provided with the motor connected with the stirring paddle, so that one culture device can be provided with a plurality of rotating speeds by setting different rotating speeds for different motors. Simultaneously, the stirring paddle not only mixes the materials and supplies oxygen, but also can realize that the materials are fermented and cultivated to generate similar shearing force with the fermentation tank through stirring, and can realize the effect of simulating the shearing force of the fermentation tank on the growth of thalli, thereby ensuring the accuracy of experimental results, and the fermentation parameters can be directly applied to industrial production.
Description
Technical Field
The utility model relates to the technical field of microorganism culture, in particular to a microorganism constant temperature culture device.
Background
At present, the liquid fermentation process is optimized in a laboratory by a constant-temperature shaking incubator, the culture mode is to inoculate a prepared culture medium and then place the inoculated culture medium in the constant-temperature shaking incubator, and then control a certain temperature and a certain rotating speed for culture.
In addition, when the fermentation process is optimized, five parameters are generally selected for optimizing the rotation speed of the incubator, so that if only one constant-temperature oscillation incubator is used, a plurality of batches of experiments are required, the experiment period is required to be prolonged, certain differences exist in each batch of seeds, and certain influence is caused on the experiment result; if five constant temperature culture devices are adopted for simultaneous operation, the fermentation parameters can be optimized, and the equipment cost is high and the occupied space is large although a certain problem can be avoided. In addition, the conventional constant temperature shaking incubator cannot simulate the influence of the shear force of the fermentation tank on the growth of the thalli, so that the fermentation parameters optimized by the conventional constant temperature shaking incubator are difficult to be directly applied to industrial production.
Disclosure of utility model
In order to solve the technical problems, the utility model provides the microorganism constant temperature culture device which can simultaneously set different rotating speeds, can simulate the influence of the shearing force of a fermentation tank on the growth of thalli, and ensures the accuracy of experimental results.
The technical scheme adopted for solving the technical problems is as follows:
The microorganism constant temperature culture device comprises a box body, wherein a display control screen is arranged on the box body, and a plurality of groups of culture components are arranged in the box body;
The culture assembly comprises a triangular flask, a stirring paddle is arranged in the triangular flask, the other end of the stirring paddle is connected with a motor, and the motor is electrically connected with the display control screen.
Preferably, a detection device is arranged in the triangular flask, and the detection device is electrically connected with the display control screen.
Preferably, the detection device comprises a temperature electrode for testing temperature, a pH electrode for testing pH and an oxygen dissolving electrode for testing oxygen dissolving content, and the output ends of the temperature electrode, the pH electrode and the oxygen dissolving electrode are connected with the display control screen.
Preferably, a heating device, a cooling device and a circulating fan are arranged in the box body, and the heating device, the cooling device and the circulating fan are electrically connected with the display control screen.
Preferably, the stirring paddle comprises a stirring shaft and a paddle arranged inside the triangular flask, one end of the stirring shaft is connected with the paddle, and the other end of the stirring shaft is connected with the motor.
Preferably, the bottle mouth of the triangular bottle is inserted with a bottle plug, and the stirring shaft is inserted with the bottle plug.
Preferably, the bottle stopper comprises a wear-resistant layer and a ventilation layer, wherein the wear-resistant layer is coated on the outer wall of the stirring shaft, and the ventilation layer is coated on the outer surface of the wear-resistant layer.
Preferably, the wear-resistant layer is made of polytetrafluoroethylene, and the breathable layer is made of breathable silica gel.
Preferably, a fixing frame is arranged in the box body, and the triangular flask, the motor and the display control screen are all arranged on the fixing frame.
Preferably, the middle part of mount is equipped with first mounting bracket, the bottom of mount is equipped with the second mounting bracket, the display control screen sets up the upper portion of mount, the motor sets up on the first mounting bracket, the triangular flask sets up on the second mounting bracket.
Compared with the prior art, the microorganism constant temperature culture device provided by the embodiment of the utility model has the beneficial effects that: through being equipped with multiunit at the inside of box and cultivateing the subassembly, every group cultivates the subassembly and all set up motor connection stirring rake alone to the accessible sets up different rotational speeds to different motors and realizes that a culture apparatus sets up a plurality of rotational speeds. Simultaneously, the stirring paddle not only mixes the materials and supplies oxygen, but also can realize that the materials are fermented and cultivated to generate similar shearing force with the fermentation tank through stirring, and can realize the effect of simulating the shearing force of the fermentation tank on the growth of thalli, thereby ensuring the accuracy of experimental results, and the fermentation parameters can be directly applied to industrial production.
Drawings
FIG. 1 is a schematic diagram showing the structure of a microorganism thermostatic culture device of the present utility model.
Fig. 2 is a schematic view of the structure of the bottle stopper of the present utility model.
Wherein: 1-box, 2-display control screen, 3-triangular flask, 4-stirring paddle, 5-motor, 6-temperature electrode, 7-pH electrode, 8-dissolved oxygen electrode, 9-heating device, 10-cooling device, 11-circulating fan, 12-wearing layer, 13-ventilative layer, 14-first mounting bracket, 15-second mounting bracket, 16-mount.
Detailed Description
The following describes in further detail the embodiments of the present utility model with reference to the drawings and examples. The following examples are illustrative of the utility model and are not intended to limit the scope of the utility model.
As shown in FIG. 1, a microorganism constant temperature culture device of a preferred embodiment of the utility model comprises a box body 1, wherein a display control screen 2 is arranged on the box body 1, and a plurality of groups of culture components are arranged in the box body 1;
The culture component comprises a triangular flask 3, a stirring paddle 4 is arranged in the triangular flask 3, the other end of the stirring paddle 4 is connected with a motor 5, the motor 5 is electrically connected with the display control screen 2, and parameters of the motor 5 can be adjusted through the display control screen 2.
According to the microorganism constant temperature culture device based on the technical characteristics, a plurality of groups of culture components are arranged in the box body 1, each group of culture components is independently provided with a motor 5 to be connected with the stirring paddle 4, and therefore one culture device can be provided with a plurality of rotating speeds by setting different rotating speeds for different motors 5. Meanwhile, the stirring paddle 54 not only uniformly mixes and supplies oxygen to the materials, but also can realize that the materials are fermented and cultured to generate similar shearing force as a fermentation tank by stirring, and can simulate the influence of the shearing force of the fermentation tank on the growth of thalli, thereby ensuring the accuracy of experimental results, and the fermentation parameters can be directly applied to industrial production.
In addition, the conventional constant-temperature shaking incubator adopts a shaking bottle, the filling amount is only 10-20% of the volume of the shaking bottle when the fermentation parameters are optimized, and the filling amount of the fermentation tank for production is about 70% of the tank body, so that the production of the fermentation tank cannot be simulated, and a large amount of parameter optimization of the fermentation tank is required after the shaking bottle is optimized in the fermentation process. The triangular flask 3 is adopted and stirred by the stirring paddle 4, so that the loading amount of the triangular flask 3 can reach about 70%, the fermentation condition of the fermentation tank can be effectively simulated, and a theoretical basis is provided for the amplification of the fermentation process.
In this embodiment, a detection device is disposed in the triangular flask 3, and is electrically connected with the display control screen 2, and the detection device detects various parameters of the culture medium in the triangular flask 3, and adjusts the parameters of the culture medium through the display control screen 2, so as to ensure that the parameters of the culture medium are in a normal range.
Specifically, the detection device comprises a temperature electrode 6 for testing temperature, a pH electrode 7 for testing pH and an oxygen dissolving electrode 8 for testing oxygen dissolving content, wherein the output ends of the temperature electrode 6, the pH electrode 7 and the oxygen dissolving electrode 8 are connected with the display control screen 2. The electrodes can display the temperature, the PH value and the dissolved oxygen parameters in the triangular flasks 3 to the display control screen 2, and then the temperature and the rotating speed can be adjusted through the display control screen 2. The rotating speed is used for adjusting the dissolved oxygen amount, and the PH value needs to be adjusted by adding corresponding solution.
When the temperature needs to be adjusted, the inside of the box body 1 is provided with a heating device 9, a cooling device 10 and a circulating fan 11, the heating device 9, the cooling device 10 and the circulating fan 11 are electrically connected with the display control screen 2, and the temperature of the box body 1 is controlled by controlling the opening and stopping of the heating device 9, the cooling device 10 and the circulating fan 11 through the parameters of the display control screen 2.
In this embodiment, the stirring paddle 4 includes a stirring shaft and a blade disposed inside the triangular flask 3, one end of the stirring shaft is connected to the blade, and the other end is connected to the motor 5. The motor 5 is connected through a connecting shaft. The paddles of the stirring paddle 4 are foldable paddles (in the prior art), the triangular flask 3 is placed before liquid filling, the liquid filling is convenient, and the paddles are unfolded after the liquid filling.
In this embodiment, as shown in fig. 2, the bottle mouth of the triangular bottle 3 is inserted with a bottle stopper, and the stirring shaft is inserted with the bottle stopper. Specifically, the bottle stopper comprises a wear-resistant layer 12 and a ventilation layer 13, wherein the wear-resistant layer 12 is coated on the outer wall of the stirring shaft, and is rubbed with the wear-resistant layer 12 when the stirring shaft rotates, so that the strength and the service life of the bottle stopper are ensured, and the wear-resistant layer 12 is made of polytetrafluoroethylene. The breathable layer 13 is coated on the outer surface of the wear-resistant layer 12, so that the breathability of the inside of the triangular flask 3 is guaranteed, and the breathable layer 13 is made of breathable silica gel.
In this embodiment, the inside of the box 1 is provided with a fixing frame 16, and the triangular flask 3, the motor 5 and the display control screen 2 are all arranged on the fixing frame 16, so that the triangular flask 3, the motor 5 and the display control screen 2 are convenient to install. Specifically, the middle part of mount 16 is equipped with first mounting bracket 14, the bottom of mount 16 is equipped with second mounting bracket 15, display control screen 2 sets up the upper portion of mount 16, motor 5 sets up on the first mounting bracket 14, the triangular flask 3 sets up on the second mounting bracket 15.
In summary, the utility model has the following advantages: the electrodes are arranged in the triangular flask 3, so that the fermentation condition of each flask can be monitored in real time in the fermentation process, and fermentation parameters of each flask can be adjusted and optimized in time according to the monitored data. The traditional constant-temperature shaking incubator cannot monitor the fermentation condition in the shake flask in time, so that the sample detection is needed in time, and the workload of experimenters is greatly increased.
The motors 5 of the culture device can be independently controlled, so that fermentation can be simultaneously carried out at a plurality of different rotating speeds each time, and the culture device is greatly helpful for researching the aerobic condition of the thalli and the sensitivity to shearing force. And the traditional constant temperature shaking culture device can only set one rotating speed in the same device.
The culture device provided by the utility model can replace a fermentation tank to perform fermentation process optimization in the early stage, can realize parameter monitoring of pH, dissolved oxygen, temperature and the like, can achieve the effect of 70% of loading amount, and simultaneously avoids the defects of high cost, difficult operation, large occupied area and the like of the traditional parallel fermentation tank.
The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.
Claims (10)
1. A microorganism constant temperature culture device, which is characterized in that: the device comprises a box body, wherein a display control screen is arranged on the box body, and a plurality of groups of culture components are arranged in the box body;
The culture assembly comprises a triangular flask, a stirring paddle is arranged in the triangular flask, the other end of the stirring paddle is connected with a motor, and the motor is electrically connected with the display control screen.
2. The microbial thermostatic culture apparatus according to claim 1, wherein: and a detection device is arranged in the triangular flask and is electrically connected with the display control screen.
3. The microbial thermostatic culture apparatus according to claim 2, wherein: the detection device comprises a temperature electrode for testing temperature, a pH electrode for testing pH and an oxygen dissolving electrode for testing oxygen dissolving content, wherein the output ends of the temperature electrode, the pH electrode and the oxygen dissolving electrode are connected with the display control screen.
4. The microbial thermostatic culture apparatus according to claim 2, wherein: the inside of box is equipped with heating device, cooling device and circulating fan, heating device cooling device with circulating fan all with the display control screen electricity is connected.
5. The microbial thermostatic culture apparatus according to claim 1, wherein: the stirring paddle comprises a stirring shaft and paddles arranged inside the triangular flask, one end of the stirring shaft is connected with the paddles, and the other end of the stirring shaft is connected with the motor.
6. The microbial thermostatic culture apparatus according to claim 5, wherein: the bottle mouth of the triangular bottle is inserted with a bottle plug, and the stirring shaft is inserted with the bottle plug.
7. The microbial thermostatic culture apparatus according to claim 6, wherein: the bottle stopper comprises a wear-resistant layer and a ventilation layer, wherein the wear-resistant layer is coated on the outer wall of the stirring shaft, and the ventilation layer is coated on the outer surface of the wear-resistant layer.
8. The microbial thermostatic culture apparatus according to claim 7, wherein: the wear-resisting layer is made of polytetrafluoroethylene, and the breathable layer is made of breathable silica gel.
9. The microbial thermostatic culture apparatus according to any one of claims 1 to 8 wherein: the inside of box is equipped with the mount, the triangular flask the motor reaches the display control screen all sets up on the mount.
10. The microbial thermostatic culture apparatus according to claim 9, wherein: the middle part of mount is equipped with first mounting bracket, the bottom of mount is equipped with the second mounting bracket, the display control screen sets up the upper portion of mount, the motor sets up on the first mounting bracket, the triangular flask sets up on the second mounting bracket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322710153.2U CN220999635U (en) | 2023-10-10 | 2023-10-10 | Microorganism constant temperature culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322710153.2U CN220999635U (en) | 2023-10-10 | 2023-10-10 | Microorganism constant temperature culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220999635U true CN220999635U (en) | 2024-05-24 |
Family
ID=91114372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322710153.2U Active CN220999635U (en) | 2023-10-10 | 2023-10-10 | Microorganism constant temperature culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220999635U (en) |
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2023
- 2023-10-10 CN CN202322710153.2U patent/CN220999635U/en active Active
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