CN216639525U

CN216639525U - Cell culture bottle

Info

Publication number: CN216639525U
Application number: CN202123266342.2U
Authority: CN
Inventors: 苏依拉其木格; 付英; 苏布道; 安鲁斯; 包玉荣
Original assignee: Affiliated Hospital Of Inner Mongolia Medical University Cardiovascular Research Institute Of Inner Mongolia Autonomous Region
Current assignee: Affiliated Hospital Of Inner Mongolia Medical University Cardiovascular Research Institute Of Inner Mongolia Autonomous Region
Priority date: 2021-12-23
Filing date: 2021-12-23
Publication date: 2022-05-31
Anticipated expiration: 2031-12-23

Abstract

The utility model discloses a cell culture bottle which comprises a bottle body, wherein the top of the bottle body is detachably connected with a bottle cap, a motor is embedded in the bottle cap, a stirring shaft is fixedly connected onto an output shaft of the motor, penetrates through the bottom surface of the bottle cap and extends into the bottle body, the stirring shaft is rotatably connected with the bottle cap through a sealing bearing, a stirring paddle is detachably connected onto the stirring shaft, a liquid inlet pipe is fixedly connected onto one side of the bottle body, a liquid outlet pipe is fixedly connected onto the other side of the bottle body, and pipe caps are respectively in threaded connection onto the liquid inlet pipe and the liquid outlet pipe. When the cell culture, inject culture solution and cell tissue liquid in the bottle, then starter motor, the motor drives the (mixing) shaft and rotates, and the (mixing) shaft drives the stirring rake and stirs culture solution and cell tissue liquid, makes culture solution and cell tissue liquid at the bottle inner loop flow, effectively avoids the cell adherent growth, and it is unreasonable to have solved current cell culture bottle structure, and some cells are difficult to flow, makes its easy adherent growth's problem.

Description

Cell culture bottle

Technical Field

The utility model relates to the technical field of culture bottles, in particular to a cell culture bottle.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.

In the cell culture technology, there is a suspension culture method, in which cells are suspended in a culture medium and are not allowed to grow adherently. Just need use the cell culture bottle when carrying out suspension culture, current cell culture bottle structure is unreasonable, and some cells are difficult to flow, make it grow adherent to the wall easily, consequently, current cell culture bottle still has higher probability to make the cell grow adherent to the wall, can't satisfy the suspension culture requirement.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a cell culture bottle to solve the problems in the prior art.

In order to achieve the purpose, the utility model provides the following scheme: the utility model provides a cell culture bottle which comprises a bottle body, wherein a bottle cap is detachably connected to the top of the bottle body, a motor is embedded in the bottle cap, a stirring shaft is fixedly connected to an output shaft of the motor, penetrates through the bottom surface of the bottle cap and extends into the bottle body, the stirring shaft is rotatably connected with the bottle cap through a sealing bearing, a stirring paddle is detachably connected to the stirring shaft, a liquid inlet pipe is fixedly connected to one side of the bottle body, a liquid outlet pipe is fixedly connected to the other side of the bottle body, pipe caps are respectively connected to the liquid inlet pipe and the liquid outlet pipe in a threaded manner, a base is fixedly installed at the bottom end of the bottle body, and a telescopic adsorption assembly is installed at the bottom of the base.

Preferably, the stirring rake includes the elastic sleeve, elastic sleeve both sides fixed mounting has the elasticity paddle, the elastic sleeve cover is established the (mixing) shaft outside, the (mixing) shaft with elastic sleeve sliding contact, and with elastic sleeve interference fit.

Preferably, the number of the stirring paddles is 1-3.

Preferably, a plurality of convex strips are fixedly arranged on the inner side wall of the bottle body.

Preferably, a plurality of air holes are formed in the top end of the tube cover, a hydrophobic air-permeable pad is fixedly mounted at the bottom of the tube cover, and the hydrophobic air-permeable pad is located between the tube cover and the liquid inlet pipe or the liquid outlet pipe.

Preferably, the power source is embedded in the top of the bottle cap, a control switch is fixedly mounted at the top end of the bottle cap, and the control switch is electrically connected with the power source and the motor respectively.

Preferably, flexible adsorption component includes the mounting groove, the mounting groove is seted up in the base bottom surface, the mounting groove top is inlayed and is equipped with electric putter, electric putter's piston end fixedly connected with mounting panel, mounting panel bottom end fixedly mounted has a plurality of sucking discs, electric putter with control switch electric connection.

Preferably, the number of the suckers is 3-6

The utility model discloses the following technical effects:

when the cell culture bottle is used for culturing cells, culture solution and cell tissue solution are injected into the bottle body, then the motor is started, the motor drives the stirring shaft to rotate, and the stirring shaft drives the stirring paddle to stir the culture solution and the cell tissue solution, so that the culture solution and the cell tissue solution circularly flow in the bottle body, the adherent growth of the cells is effectively avoided, and the problems that the existing cell culture bottle is unreasonable in structure, some cells are difficult to flow, and the cells are easy to adhere to the wall to grow are solved.

The motor is embedded in the bottle cap, when the motor runs, vibration can be generated to a certain degree, the bottom of the bottle body is easy to be unstable and can topple, the base can increase the weight of the bottom of the bottle body, the gravity center of the whole cell culture bottle is reduced, the cell culture bottle is not prone to toppling, meanwhile, the telescopic adsorption assembly is arranged at the bottom of the base, the base can be adsorbed on the workbench or the culture box, and the cell culture bottle can stand stably.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

FIG. 1 is a schematic view of the external structure of a cell culture flask;

FIG. 2 is a schematic view of the internal structure of the cell culture flask;

FIG. 3 is a top plan view of the tube cover;

FIG. 4 is a schematic top view of the control switch;

in the figure: 1. a bottle body; 2. a bottle cap; 3. a motor; 4. a stirring shaft; 5. sealing the bearing; 6. an elastic sleeve; 7. a liquid inlet pipe; 8. a liquid outlet pipe; 9. a tube cover; 10. an elastic paddle; 11. a convex strip; 12. a power source; 13. a control switch; 14. air holes are formed; 15. a hydrophobic breathable pad; 16. a base; 17. mounting grooves; 18. an electric push rod; 19. mounting a plate; 20. and (4) sucking discs.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description thereof.

The utility model provides a cell culture bottle, which comprises a bottle body 1, wherein the bottle body 1 is made of transparent materials and can conveniently penetrate through cell culture conditions, a bottle cap 2 is detachably connected to the top of the bottle body 1, the bottle body 1 and the bottle cap 2 are connected through threads, the disassembly and the assembly are convenient, meanwhile, the sealing performance can be ensured, a motor 3 is embedded in the bottle cap 2, a stirring shaft 4 is fixedly connected to an output shaft of the motor 3, the stirring shaft 4 extends into the bottle body 1 through the bottom surface of the bottle cap 2, the stirring shaft 4 is rotatably connected with the bottle cap 2 through a sealing bearing 5, the rotation of the stirring shaft 4 is convenient, meanwhile, the moisture in the bottle body is prevented from entering the motor 3, the motor 3 is prevented from being damped and damaged, a stirring paddle is detachably connected to the stirring shaft 4, when cells are cultured, culture solution and cell tissue solution are injected into the bottle body 1, then the motor 3 is started, the motor 3 drives the stirring shaft 4 to rotate, the stirring paddle is driven by the stirring shaft 4 to stir the culture solution and the cell tissue solution, the culture solution and the cell tissue solution circularly flow in the bottle body 1, the adherent growth of cells is effectively avoided, one side of the bottle body 1 is fixedly connected with a liquid inlet pipe 7, the liquid inlet pipe 7 is used for supplementing the culture solution, the other side of the bottle body 1 is fixedly connected with a liquid outlet pipe 8, the liquid outlet pipe 8 is used for cleaning up the waste culture solution, pipe covers 9 are respectively in threaded connection with the liquid inlet pipe 7 and the liquid outlet pipe 8, pollutants are prevented from entering the bottle body 1 from the liquid inlet pipe 7 and the liquid outlet pipe 8, a base 16 is fixedly arranged at the bottom end of the bottle body 1, a telescopic adsorption component is arranged at the bottom of the base 16, a motor 3 is embedded in the bottle cover 2, when the motor 3 runs, vibration of a certain degree can be generated, the bottom of the bottle body 1 is easy to be unstable and can be toppled over, the base 16 can increase the weight at the bottom of the bottle body 1, the gravity center of the whole cell culture bottle is reduced, so that the cell culture bottle is not easy to topple over, and meanwhile, the telescopic adsorption component is arranged at the bottom of the base 16, the base 16 can be attached to a table or incubator to enable the cell culture flask to stand stably.

Further optimization scheme, the stirring rake includes elastic sleeve 6, elastic paddle 10 of 6 both sides fixed mounting of elastic sleeve, the cover of elastic sleeve 6 is established in the (mixing) shaft 4 outside, the (mixing)

shaft

4 and 6 sliding contact of elastic sleeve, and with 6 interference fit of elastic sleeve, stirring rake quantity is 1-3, can be according to the quantity of highly selective installation stirring rake of culture solution, it is very convenient to install and dismantle the stirring rake, only need to pull elastic paddle 10 to both sides with the hand, then with 6 cover of elastic sleeve to the (mixing) shaft 4 outside can, because (mixing)

shaft

4 and 6 interference fit of elastic sleeve, so elastic sleeve 6 can be firm the cover on (mixing) shaft 4, can not drop because of the (mixing) shaft 4 is rotatory, the (mixing) shaft 4 transfers with 6 sliding contact of elastic sleeve, can be according to the demand, very convenient adjustment elastic sleeve 6's height.

Further optimize the scheme, fixed mounting has a plurality of sand grips 11 on the 1 inside wall of bottle, and when the stirring rake was rotatory, the culture solution produced the striking with sand grip 11, made the culture solution produce the vortex, made culture solution and cell tissue liquid mix more evenly, increased mobility simultaneously, prevented that the cell from adhering to the wall and growing.

Further optimization scheme, a plurality of bleeder vents 14 have been seted up on the tube cap 9 top, and tube cap 9 bottom fixed mounting has hydrophobic ventilative pad 15, and hydrophobic ventilative pad 15 is located between tube cap 9 and feed liquor pipe 7 or drain pipe 8, makes in external air can see through hydrophobic ventilative pad 15 gets into bottle 1, is fit for the growth of oxygen-like cell, if cultivates anaerobic cell, then fills up 15 with hydrophobic ventilative and change for sealed the pad, isolated outside air.

Further optimization scheme, 2 tops of bottle lid are inlayed and are equipped with

power

12, and 2 top fixed mounting of bottle lid have control switch 13, and control switch 13 respectively with power 12 and motor 3 electric connection, power 12 provides the energy for motor 3, control switch 13 control motor 3 switch and rotational speed.

Further optimize the scheme, flexible absorption assembly includes mounting groove 17, mounting groove 17 is seted up in 16 bottom surfaces of base, mounting groove 17 top is inlayed and is equipped with electric putter 18, electric putter 18's piston end fixedly connected with mounting panel 19, mounting panel 19 bottom fixed mounting has a plurality of sucking discs 20, electric putter 18 and control switch 13 electric connection, when needing to use sucking disc 20, start electric putter 18, its piston rod promotes mounting panel 19 and descends, make sucking disc 20 stretch out mounting groove 17, sucking disc 20 can with adsorb on workstation or incubator, when need not use sucking disc 20, start electric putter 18, its piston rod stimulates mounting panel 19 to rise, make sucking disc 20 accomodate into in mounting groove 17, base 16 directly with workstation or incubator contact can, sucking disc 20 quantity is 3-6, in order to ensure the absorptive stability of sucking disc.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. A cell culture flask, characterized in that: comprises a bottle body (1), the top of the bottle body (1) is detachably connected with a bottle cap (2), a motor (3) is embedded in the bottle cap (2), a stirring shaft (4) is fixedly connected to an output shaft of the motor (3), the stirring shaft (4) penetrates through the bottom surface of the bottle cap (2) and extends into the bottle body (1), the stirring shaft (4) is rotationally connected with the bottle cap (2) through a sealing bearing (5), the stirring shaft (4) is detachably connected with a stirring paddle, one side of the bottle body (1) is fixedly connected with a liquid inlet pipe (7), a liquid outlet pipe (8) is fixedly connected with the other side of the bottle body (1), pipe covers (9) are respectively connected with the liquid inlet pipe (7) and the liquid outlet pipe (8) in a threaded manner, the bottom end of the bottle body (1) is fixedly provided with a base (16), and the bottom of the base (16) is provided with a telescopic adsorption component.

2. The cell culture flask according to claim 1, wherein: the stirring rake includes elastic sleeve (6), elastic sleeve (6) both sides fixed mounting has elasticity paddle (10), elastic sleeve (6) cover is established (mixing) shaft (4) outside, mixing shaft (4) with elastic sleeve (6) sliding contact, and with elastic sleeve (6) interference fit.

3. The cell culture flask according to claim 1, wherein: the number of the stirring paddles is 1-3.

4. The cell culture flask according to claim 1, wherein: a plurality of convex strips (11) are fixedly arranged on the inner side wall of the bottle body (1).

5. The cell culture flask according to claim 1, wherein: the pipe cover is characterized in that a plurality of air holes (14) are formed in the top end of the pipe cover (9), a hydrophobic air-permeable pad (15) is fixedly mounted at the bottom of the pipe cover (9), and the hydrophobic air-permeable pad (15) is located between the pipe cover (9) and the liquid inlet pipe (7) or the liquid outlet pipe (8).

6. The cell culture flask according to claim 1, wherein: bottle lid (2) top is inlayed and is equipped with power (12), bottle lid (2) top fixed mounting has control switch (13), control switch (13) respectively with power (12) and motor (3) electric connection.

7. The cell culture flask of claim 6, wherein: flexible adsorption component includes mounting groove (17), mounting groove (17) are seted up in base (16) bottom surface, mounting groove (17) top is inlayed and is equipped with electric putter (18), the piston end fixedly connected with mounting panel (19) of electric putter (18), mounting panel (19) bottom fixed mounting has a plurality of sucking discs (20), electric putter (18) with control switch (13) electric connection.

8. The cell culture flask of claim 7, wherein: the number of the suckers (20) is 3-6.