CN208395195U - A kind of high-performance cell culture reactor - Google Patents
A kind of high-performance cell culture reactor Download PDFInfo
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- CN208395195U CN208395195U CN201820317572.6U CN201820317572U CN208395195U CN 208395195 U CN208395195 U CN 208395195U CN 201820317572 U CN201820317572 U CN 201820317572U CN 208395195 U CN208395195 U CN 208395195U
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- swash plate
- piston shoes
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- tank body
- central axis
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Abstract
The utility model provides a kind of high-performance cell culture reactor, and the utility model structure is simple, has the characteristics that cell culture efficiency is high；Main agitator, secondary agitator rotation are respectively driven by the first swash plate body, the second swash plate body, circular motion is converted by upper and lower linear reciprocating motion, avoids damaging cell because shear velocity is excessive；By the three-dimensional stirring structure of main agitator, axial stirring range is increased；Laterally stirring range is increased by secondary agitator, shunting function is played using the curvilinear structures and dispersion slot of secondary agitator, improves mixing effect；The bubble that tank body top is effectively removed by the rotation of main defoaming part, avoids the cell of culture from rupturing because of bubble, provides the efficiency of cell culture；The bubble that tank body lower part is effectively removed by the defoaming disk to pump, avoids the generation of bubble, it is ensured that the eubolism of cell avoids cell death from causing energy waste.
Description
Technical field
The utility model relates to biological plant technical fields, and in particular to a kind of high-performance cell culture reactor.
Background technique
Modern biotechnology is generally thought to comprise technique for gene engineering, cell engineering, enzyme engineering technology and fermentation work
Journey technology, and the development of these technologies nearly all has substantial connection with cell culture, especially in the development of field of medicaments, cell
Culture has the function of special and value, for example genetically engineered drug or vaccine are much by thin during researching and producing
Born of the same parents cultivate to realize.Cell culture plays very crucial central role, cell in the development of entire biotechnology industry
Culture, which refers to, simulates tumor growth environment for the cell for acquiring in-vivo tissue, is placed on sterile, certain nutritional condition, suitable temperature
Under degree and pH value, make its growth, breeding, and maintain a kind of technology of its structure and function.Bioreactor is cell culture
In one of essential equipment, bioreactor is also referred to as biological reactor, using biological function possessed by enzyme or organism,
The device of biochemical reaction is carried out in vitro.In the prior art, it needs to carry out cell culture by high speed of agitator effective
Mixing, increase dissolved oxygen, but the shearing force that stirring slurry under high speed conditions generates can cause serious injury to cell, hold
Cell culture failure is easily led to, while the bubble generated will lead to cellular damage even death in cell culture fluid.
Utility model content
In order to solve the problems in the existing technology, the utility model provides a kind of high-performance cell culture reaction
Device has the characteristics that structure is simple, cell culture efficiency is high.
The technical solution that the utility model solves the above problems are as follows: a kind of high-performance cell culture reactor, including tank body,
First agitating device；Tank body top is equipped with feed inlet, and tank body bottom end is equipped with discharge port, and the first agitating device, the are equipped in tank body
Two agitating devices, the first agitating device are located at tank body top, and the second agitating device is located at tank body lower part；
First agitating device includes the first electric cylinder, the first swash plate body, main agitator, and the first electric cylinder is fixed on tank body
Outside, the first oblique disk structure includes drive rod, the first swash plate, and drive rod one end is connected with the first electronic cylinder piston rod, and the other end is set
There is the first piston shoes seat, the first piston shoes seat is spherical structure；First swash plate upper surface is equipped with the first piston shoes, and the first piston shoes and first are tiltedly
It is slidably connected between disk, forms plane contact kinematic pair, the first piston shoes are spherical structure；First piston shoes seat is located at the first piston shoes
It is interior, connection is rolled between the first piston shoes seat and the first piston shoes, forms sphere-contact kinematic pair；First swash plate bottom is equipped with main stirring
Axis, main agitating shaft central axis are overlapped with the first swash plate central axis, and main agitating shaft is main by being connected between supporting element and tank body
Main agitator there are two being set on agitating shaft；First swash plate external cylindrical surface is equipped with main defoaming part, and main defoaming part is ring structure, main
It defoams part and is equipped with multiple defoaming holes；
Second agitating device includes the second electric cylinder, the second swash plate body, secondary agitator, and the second electric cylinder is located at tank body
Outside；Second swash plate body includes drive rod, the second swash plate, and the second swash plate is equipped with multiple defoaming holes, and the second swash plate upper surface is set
Have a secondary agitating shaft, secondary agitating shaft by being connected between supporting element and tank body, set on secondary agitating shaft there are two secondary agitator；Second tiltedly
The upper surface and lower end surface of disk are respectively equipped with four the second piston shoes, and the second piston shoes surround the second swash plate central axis annular array,
It is slidably connected between second piston shoes and the second swash plate, forms plane contact kinematic pair, the second piston shoes are spherical structure；Each second
Piston shoes are equipped with drive rod, and one end that drive rod is connected with the second piston shoes is equipped with the second piston shoes seat, and the second piston shoes seat is spherical surface knot
Structure, the second piston shoes are located in the second piston shoes seat, and connection is rolled between the second piston shoes seat and the second piston shoes, form sphere-contact movement
It is secondary；Each drive rod is equipped with defoaming disk, and defoaming disk is equipped with multiple defoaming holes, and one of drive rod is far from the second swash plate
One end is connected with the second electronic cylinder piston rod.
The main agitator includes first-class parts, second parts, and first-class parts are located above second parts, the
First-class parts are circular ring structure, and first-class parts central axis is overlapped with second parts central axis；First-class parts and the
It is equipped with multiple bent blades between second-rate parts, bent blade surrounds first-class parts central axis annular array, and bent blade is far from the
The side of first-class parts central axis is equipped with arc paddle；Second parts are equipped with multiple prismatic blades, and prismatic blade is around the
Second-rate parts central axis annular array, prismatic blade is between two neighboring first-class parts.
The secondary agitator includes mounting ring, agitation leaf, and mounting ring is equipped with multiple agitation leaves, and agitation leaf surrounds mounting ring
Central axis annular array stirs the upper surface of leaf and lower end surface and is respectively equipped with the first stirring area, the second stirring area, the first stirring
Main agitating fins, secondary agitating fins are equipped in area, the second stirring area, main agitating fins and secondary agitating fins are located at agitation
The both ends of leaf；Main agitating fins side is connected with mounting ring, and the other side is curve-like, and main agitating fins are equipped with multiple dispersions
Slot；Secondary agitating fins are curve-like close to the side of main agitating fins, and secondary agitating fins are equipped with multiple dispersion slots；Main stirring wing
Agitation fin is equipped between piece and secondary agitating fins, agitation fin two sides are curve-like.
The agitation fin upper end width is L1, lower end width L2, L1 < L2.
The main agitating fins upper end width is L3, lower end width L4, L3 < L4.
The pair agitating fins upper end width is L5, lower end width L6, L5 < L6.
The first swash plate central axis is overlapped with tank body central axis, the second swash plate central axis and tank body central axis
It is overlapped.
The pair agitating shaft central axis is overlapped with tank body central axis, defoam disk center's axis and tank body central axis it
Between be overlapped.
The main agitating shaft, secondary agitating shaft are connected between supporting element by rolling bearing.
The drive rod, drive rod pass through support frame and are connected with tank body.
The utility model has the utility model has the advantages that the utility model structure is simple, has cell culture efficiency height, stirs evenly
The characteristics of；Main agitator, secondary agitator rotation are respectively driven by the first swash plate body, the second swash plate body, it will be upper and lower past
Linear motion is converted into circular motion, provides condition for slowly running for blender, avoids because shear velocity is excessive to cell
It damages；By the three-dimensional stirring structure of main agitator, axial stirring range is increased, reactor temperature and nutrients are made
Matter concentration is unified, guarantees the uniformity of tank body top stirring；Laterally stirring range is increased by secondary agitator, is stirred using pair
The curvilinear structures and dispersion slot of device play shunting function, avoid tank base from material deposition and stirring dead angle occur, improve stirring
Effect；The bubble that tank body top is effectively removed by the rotation of main defoaming part, avoids the cell of culture from rupturing because of bubble, provides
The efficiency of cell culture；The bubble that tank body lower part is effectively removed by the defoaming disk to pump, avoids the production of bubble
It is raw, it is ensured that the eubolism of cell avoids cell death from causing energy waste.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model.
Fig. 2 is that the first piston shoes of the utility model and the cooperation of the first piston shoes seat are schemed.
Fig. 3 is the first oblique disk structure of the utility model schematic diagram.
Fig. 4 is the second oblique disk structure of the utility model schematic diagram.
Fig. 5 is the utility model main agitator structural schematic diagram.
Fig. 6 is the utility model secondary agitator structural schematic diagram.
In figure: 1- tank body, the first agitating device of 2-, the second agitating device of 3-, 4- feed inlet, 5- discharge port, the first electricity of 6-
Dynamic cylinder, the first swash plate body of 7-, 9- drive rod, the first swash plate of 10-, 11- the first piston shoes seat, the first piston shoes of 12-, the main stirring of 13-
Axis, 14- main agitator, the main defoaming part of 15-, 16- defoam hole, the second electric cylinder of 17-, the second swash plate body of 18-, the stirring of 19- pair
Device, 20- drive rod, the second swash plate of 21-, 23- the second piston shoes seat, 24- defoaming disk, 25- support frame, 26- supporting element, 27- pair are stirred
Mix axis, the first-class parts of 101-, 102- second parts, 104- arc paddle, 105- song blade, 106- prismatic blade, 107-
Mounting ring, 108- stir leaf, the main agitating fins of 109-, 110- pair agitating fins, the first stirring area 111-, the stirring of 112- second
Area, 113- dispersion slot, 114- stir fin.
Specific embodiment
With reference to the accompanying drawing and specific embodiment is further described the utility model.
A kind of high-performance cell culture reactor, including tank body 1, the first agitating device 2；1 top of tank body is equipped with feed inlet
4,1 bottom end of tank body is equipped with discharge port 5, is equipped with the first agitating device 2, the second agitating device 3, the first agitating device 2 in tank body 1
In 1 top of tank body, the second agitating device 3 is located at 1 lower part of tank body；
First agitating device 2 includes the first electric cylinder 6, the first swash plate body 7, main agitator 14, and the first electric cylinder 6 is fixed
Outside tank body 1,10 structure of the first swash plate includes drive rod 9, the first swash plate 10,9 one end of drive rod and 6 piston rod of the first electric cylinder
It is connected, the other end is equipped with the first piston shoes seat 11, and the first piston shoes seat 11 is spherical structure；It is sliding that first swash plate, 10 upper surface is equipped with first
Boots 12 are slidably connected between first piston shoes 12 and the first swash plate 10, form plane contact kinematic pair, and the first piston shoes 12 are spherical surface knot
Structure；First piston shoes seat 11 is located in the first piston shoes 12, and connection is rolled between the first piston shoes seat 11 and the first piston shoes 12, forms spherical surface
Contact movement pair；First swash plate, 10 bottom is equipped with main agitating shaft 13, main 13 central axis of agitating shaft and 10 central axis of the first swash plate
Line is overlapped, main agitating shaft 13 by being connected between supporting element 26 and tank body 1, set on main agitating shaft 13 there are two main agitator 14；
First swash plate, 10 external cylindrical surface is equipped with main defoaming part 15, and main defoaming part 15 is ring structure, and main defoaming part 15 is equipped with multiple
Defoam hole 16；
Second agitating device 3 includes the second electric cylinder 17, the second swash plate body 18, secondary agitator 19, the second electric cylinder 17
Outside tank body 1；Second swash plate body 18 includes drive rod 20, the second swash plate 21, and the second swash plate 21 is equipped with multiple defoaming holes
16,21 upper surface of the second swash plate is equipped with secondary agitating shaft 27, and secondary agitating shaft 27 by being connected between supporting element 26 and tank body 1, stir by pair
Mix secondary agitator 19 there are two setting on axis 27；The upper surface and lower end surface of second swash plate 21 are respectively equipped with four the second piston shoes, the
Two piston shoes surround 21 central axis annular array of the second swash plate, are slidably connected between the second piston shoes and the second swash plate 21, are formed flat
Face contact kinematic pair, the second piston shoes are spherical structure；Each second piston shoes are equipped with drive rod 20, drive rod 20 and the second piston shoes
Connected one end is equipped with the second piston shoes seat 23, and the second piston shoes seat 23 is spherical structure, and the second piston shoes are located in the second piston shoes seat 23,
Connection is rolled between second piston shoes seat 23 and the second piston shoes, forms sphere-contact kinematic pair；Each drive rod 20 is equipped with defoaming
Disk 24, defoaming disk 24 are equipped with multiple defoaming holes 16, the one end of one of drive rod 20 far from the second swash plate 21 and the second electricity
Dynamic 17 piston rod of cylinder is connected.
The main agitator 14 includes first-class parts 101, second parts 102, and first-class parts 101 are located at second
102 top of parts, first-class parts 101 are circular ring structure, first-class 101 central axis of parts and 102 center of second parts
Axis is overlapped；Multiple bent blades 105 are equipped between first-class parts 101 and second parts 102, bent blade 105 surrounds first
Circulate 101 central axis annular array of part, and bent blade 105 is stirred far from the side of first-class 101 central axis of parts equipped with arc
Mix leaf 104；Second parts 102 are equipped with multiple prismatic blades 106, and prismatic blade 106 surrounds 102 central axis wire loop of second parts
Shape array, prismatic blade 106 is between two neighboring first-class parts 101.
The secondary agitator 19 includes mounting ring 107, agitation leaf 108, and mounting ring 107 is equipped with multiple agitation leaves 108, stirs
Movable vane 108 surrounds 107 central axis annular array of mounting ring, and the upper surface of agitation leaf 108 is respectively equipped with first with lower end surface and stirs
Area 111, the second stirring area 112 are mixed, main agitating fins 109, secondary stirring are equipped on the first stirring area 111, the second stirring area 112
Fin 110, main agitating fins 109 are located at the both ends of agitation leaf 108 with secondary agitating fins 110；Main 109 side of agitating fins
It is connected with mounting ring 107, the other side is curve-like, and main agitating fins 109 are equipped with multiple dispersion slots 113；Secondary agitating fins 110
It is curve-like close to the side of main agitating fins 109, secondary agitating fins 110 are equipped with multiple dispersion slots 113；Main agitating fins
Agitation fin 114 is equipped between 109 and secondary agitating fins 110, agitation 114 two sides of fin are curve-like.
114 upper end width of agitation fin is L1, lower end width L2, L1 < L2.
109 upper end width of main agitating fins is L3, lower end width L4, L3 < L4.
Pair 110 upper end width of agitating fins is L5, lower end width L6, L5 < L6.
First swash plate, 10 central axis is overlapped with 1 central axis of tank body, in 21 central axis of the second swash plate and tank body 1
Mandrel line is overlapped.
Pair 27 central axis of agitating shaft is overlapped with 1 central axis of tank body, defoaming 24 central axis of disk and 1 center of tank body
It is overlapped between axis.
The main agitating shaft 13, secondary agitating shaft 27 are connected between supporting element 26 by rolling bearing.
The drive rod 9, drive rod 20 are connected by support frame 25 with tank body 1.
In use, the first electric cylinder 6 drives drive rod 9 to pump, so that drive rod 9 drives the first swash plate 10
Rotation, main agitator 14 rotate, and main agitator 14 is stirred the material in reactor, the three-dimensional stirring knot of main agitator 14
Structure increases axial stirring range, keeps reactor temperature and nutrient concentrations unified, guarantees the equal of 1 top of tank body stirring
Even property, while stirring, the main defoaming part 15 of rotation effectively removes the bubble on 1 top of tank body, avoids the cell of culture because of bubble
And it ruptures；
Second electric cylinder 17 drives drive rod 20 to pump, so that drive rod 20 drives 21 turns of the second swash plate
Dynamic, secondary agitator 19 rotates, and secondary agitator 19 is stirred the material in reactor, increases transverse direction by secondary agitator 19
Range is stirred, shunting function is played using the curvilinear structures and dispersion slot 113 of secondary agitator 19,1 bottom of tank body is avoided object occur
Material deposition and stirring dead angle, improve mixing effect；While stirring, the defoaming disk 24 to pump effectively removes tank body 1
The bubble of lower part, it is ensured that the eubolism of cell avoids cell death from causing energy waste.
Many other changes and modifications may be made without departing from the spirit and scope of the utility model.It should be appreciated that this reality
It is not limited to specific embodiment with novel, the scope of the utility model is defined by the following claims.
Claims (8)
1. a kind of high-performance cell culture reactor, which is characterized in that including tank body, the first agitating device；Tank body top is equipped with
Feed inlet, tank body bottom end are equipped with discharge port, are equipped with the first agitating device, the second agitating device, the first agitating device position in tank body
In tank body top, the second agitating device is located at tank body lower part；
First agitating device includes the first electric cylinder, the first swash plate body, main agitator, and the first electric cylinder is fixed on outside tank body,
First oblique disk structure includes drive rod, the first swash plate, and drive rod one end is connected with the first electronic cylinder piston rod, and the other end is equipped with the
One piston shoes seat, the first piston shoes seat are spherical structure；First swash plate upper surface is equipped with the first piston shoes, the first piston shoes and the first swash plate it
Between be slidably connected, formed plane contact kinematic pair, the first piston shoes be spherical structure；First piston shoes seat is located in the first piston shoes, the
Connection is rolled between one piston shoes seat and the first piston shoes, forms sphere-contact kinematic pair；First swash plate bottom is equipped with main agitating shaft, main
Agitating shaft central axis is overlapped with the first swash plate central axis, and main agitating shaft between supporting element and tank body by being connected, main stirring
Main agitator there are two being set on axis；First swash plate external cylindrical surface is equipped with main defoaming part, and main defoaming part is ring structure, main defoaming
Part is equipped with multiple defoaming holes；
Second agitating device includes the second electric cylinder, the second swash plate body, secondary agitator, and the second electric cylinder is located at outside tank body；The
Two swash plate bodies include drive rod, the second swash plate, and the second swash plate is equipped with multiple defoaming holes, and the second swash plate upper surface is stirred equipped with pair
Mix axis, secondary agitating shaft by being connected between supporting element and tank body, set on secondary agitating shaft there are two secondary agitator；Second swash plate it is upper
End face and lower end surface are respectively equipped with four the second piston shoes, and the second piston shoes surround the second swash plate central axis annular array, and second is sliding
It is slidably connected between boots and the second swash plate, forms plane contact kinematic pair, the second piston shoes are spherical structure；On each second piston shoes
Equipped with drive rod, one end that drive rod is connected with the second piston shoes is equipped with the second piston shoes seat, and the second piston shoes seat is spherical structure, and second
Piston shoes are located in the second piston shoes seat, and connection is rolled between the second piston shoes seat and the second piston shoes, form sphere-contact kinematic pair；Each
Drive rod be equipped with defoaming disk, defoaming disk be equipped with multiple defoaming holes, the one end of one of drive rod far from the second swash plate with
Second electronic cylinder piston rod is connected；
Main agitator includes first-class parts, second parts, and first-class parts are located above second parts, first-class parts
For circular ring structure, first-class parts central axis is overlapped with second parts central axis；First-class parts and second parts
Between be equipped with multiple bent blades, bent blade surrounds first-class parts central axis annular array, and bent blade is far from first-class parts
The side of central axis is equipped with arc paddle；Second parts are equipped with multiple prismatic blades, and prismatic blade surrounds second parts
Central axis annular array, prismatic blade is between two neighboring first-class parts；
Secondary agitator includes mounting ring, agitation leaf, and mounting ring is equipped with multiple agitation leaves, and agitation leaf surrounds mounting ring central axis
Annular array stirs the upper surface of leaf and lower end surface and is respectively equipped with the first stirring area, the second stirring area, the first stirring area, second
Main agitating fins, secondary agitating fins are equipped on stirring area, main agitating fins and secondary agitating fins are located at the two of agitation leaf
End；Main agitating fins side is connected with mounting ring, and the other side is curve-like, and main agitating fins are equipped with multiple dispersion slots；Pair is stirred
It is curve-like that fin, which is mixed, close to the side of main agitating fins, and secondary agitating fins are equipped with multiple dispersion slots；Main agitating fins and pair
Agitation fin is equipped between agitating fins, agitation fin two sides are curve-like.
2. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the agitation fin upper end is wide
Degree is L1, lower end width L2, L1 < L2.
3. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the main agitating fins upper end
Width is L3, lower end width L4, L3 < L4.
4. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the pair agitating fins upper end
Width is L5, lower end width L6, L5 < L6.
5. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the first swash plate central axis
Line is overlapped with tank body central axis, and the second swash plate central axis is overlapped with tank body central axis.
6. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the pair agitating shaft central axis
Line is overlapped with tank body central axis, is defoamed and is overlapped between disk center's axis and tank body central axis.
7. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the main agitating shaft, pair are stirred
Axis is mixed to be connected between supporting element by rolling bearing.
8. a kind of high-performance cell culture reactor as described in claim 1, which is characterized in that the drive rod, drive rod
It is connected by support frame with tank body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820317572.6U CN208395195U (en) | 2018-03-08 | 2018-03-08 | A kind of high-performance cell culture reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820317572.6U CN208395195U (en) | 2018-03-08 | 2018-03-08 | A kind of high-performance cell culture reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208395195U true CN208395195U (en) | 2019-01-18 |
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ID=65064458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820317572.6U Expired - Fee Related CN208395195U (en) | 2018-03-08 | 2018-03-08 | A kind of high-performance cell culture reactor |
Country Status (1)
| Country | Link |
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| CN (1) | CN208395195U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113680242A (en) * | 2021-10-23 | 2021-11-23 | 南通汇顺化工有限公司 | Chemical industry pharmacy is with having mixed machine of gaseous stirring |
-
2018
- 2018-03-08 CN CN201820317572.6U patent/CN208395195U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113680242A (en) * | 2021-10-23 | 2021-11-23 | 南通汇顺化工有限公司 | Chemical industry pharmacy is with having mixed machine of gaseous stirring |
| CN113680242B (en) * | 2021-10-23 | 2022-03-25 | 南通汇顺化工有限公司 | Chemical industry pharmacy is with having mixed machine of gaseous stirring |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
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| CF01 | Termination of patent right due to non-payment of annual fee |