CN103184144A - Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device - Google Patents
Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device Download PDFInfo
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- CN103184144A CN103184144A CN2013100938262A CN201310093826A CN103184144A CN 103184144 A CN103184144 A CN 103184144A CN 2013100938262 A CN2013100938262 A CN 2013100938262A CN 201310093826 A CN201310093826 A CN 201310093826A CN 103184144 A CN103184144 A CN 103184144A
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- deflecting wheel
- retaining clip
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Abstract
The invention discloses a dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device. The loading device comprises a base film for the growth of an adherent cell used for being observed; two fixing clips for clamping the two sides of the base film respectively, two displacement support arms for supporting the fixing clips respectively, and a driving part, wherein the fixing clips are movably arranged on a rail, and the driving part is used for driving the displacement support arms to perform symmetrical opposite-direction displacement on the rail so as to conduct bidirectional-stretch on the base film. According to the invention, by conducting the stretch with a certain frequency and a certain range on the cell growing on the biocompatible film, morphologic change, cytoskeleton reorganization, signal transduction and other responses of the cell in different action times can be observed.
Description
Technical field
The present invention relates to a kind of cell device that is coupled, reaches biomedical research for biomechanics, mechanics-biology.
Background technology
The announcement of stress-growth relationship is the great discovery of biomechanics, and cell is the elementary cell of life.And distraction force is the principal element that determines cell deformation and function, and therefore, the experimental installation that development applies tension strain to the isolated culture cell becomes the key issue of solution mechanics-biology Coupling Research.
Along with going deep into the research of mechanical stress regulating cell biological function, developed the multiple device that cell in vitro is stretched and loads, mainly include strain loading device four-point bending beam deceleration loading device, two-way strain loading devices etc. such as vacuum (negative pressure) deceleration loading device, liquid (malleation) pressurizing device, twin shaft, but all can't realize the purpose to attached cell dynamic load online observation.
Summary of the invention
At the problem that prior art exists, the object of the present invention is to provide a kind of cell biological mechanical loading unit of dynamic bidirectional stretching original position online observation, can realize attached cell dynamic load online observation.
The cell biological mechanical loading unit of a kind of dynamic bidirectional stretching original position online observation of the present invention comprises:
Basilar membrane, growth is used for the attached cell of observation;
Retaining clip, described retaining clip are 2, respectively the both sides of clamping basilar membrane;
The displacement sway brace, described displacement sway brace is 2, is used for supporting described retaining clip respectively, described retaining clip is removably set on the guide rail;
Driving part, the opposite direction of doing symmetry at described guide rail for the described displacement sway brace of driving moves, so that described basilar membrane is carried out two-way stretch.
Preferably, described driving part comprises: drive-motor, bending and stretching arm, deflecting wheel chute, deflecting wheel and electric machine support; Drive-motor is arranged on the electric machine support, and deflecting wheel is arranged on the clutch end of described drive-motor; One end of bending and stretching arm is rotatable to be arranged on the described displacement sway brace, and the other end is rotatable to be arranged on the described deflecting wheel chute; Described deflecting wheel is embedded in described deflecting wheel chute.
Preferably, described retaining clip one of them be arranged on the described displacement sway brace by the microspur adjusting slider.
Preferably, also comprise the microspur adjusting knob of regulating the displacement of described microspur adjusting slider.
The present invention is by applying the stretching of certain frequency certain amplitude to growing in cell on the biocompatibility film (basilar membrane), can investigate metamorphosis, skeleton reorganization, and the response such as signal conduction of cell under different action times.
Description of drawings
Fig. 1 is structural representation of the present invention.
Embodiment
As shown in Figure 1, the present invention includes: basilar membrane 2, retaining clip 3, displacement sway brace 4 and driving part 7.Basilar membrane 2 growths are used for the attached cell of observation.Retaining clip 3 is 2, respectively the both sides of clamping basilar membrane 2.Displacement sway brace 4 also is 2, and corresponding and retaining clip 3 arranges, and is used for support fixation folder 3 respectively, and retaining clip 3 is removably set on the guide rail 6.The opposite direction that driving part 7 is done symmetry for drive displacement sway brace 4 at guide rail 6 moves, so that basilar membrane 2 is carried out two-way stretch.
As shown in Figure 1, in embodiments of the present invention, driving part 7 comprises: drive-motor 71, bending and stretching arm 75, deflecting wheel chute 73, deflecting wheel 74 and electric machine support 72; Drive-motor 71 is arranged on the electric machine support 3, and deflecting wheel 74 is arranged on the clutch end of drive-motor 71.One end of bending and stretching arm 75 is rotatable to be arranged on the displacement sway brace 5, and the other end is rotatable to be arranged on the deflecting wheel chute 73.Deflecting wheel 74 is embedded in deflecting wheel chute 73.
Like this, when drive-motor 71 drove deflecting wheel 74 rotations, it is mobile in the direction of arrow A that deflecting wheel 74 just drives deflecting wheel chute 73, and the opposite direction that makes bending and stretching arm 75 drive displacement sway braces 4 do symmetry at guide rail 6 moves, thus pulling basilar membrane 2.
Because being opposite directions of doing symmetry, moves displacement sway brace 4, just can guarantee that be roughly static in the process that the intermediate point on the basilar membrane 2 is stretching, like this, basilar membrane 2 is to do the two-way stretch campaign at certain frequency certain amplitude lower edge straight line, and the attached cell that grows on just can online in-situ observation basilar membrane 2 middle sections by microscope is subjected to the effect of drawing force strain under the effect that basilar membrane 2 stretches.
In addition, drive-motor 71 drives deflecting wheels 74 and does eccentric movement, the amplitude of eccentric movement determine the to seesaw size of distance.
In addition, as shown in Figure 1, for the ease of launching basilar membrane 2, one of them is arranged on retaining clip 3 on the displacement sway brace 5 by microspur adjusting slider 8, and microspur adjusting slider 8 is regulated displacements by microspur adjusting knob 9.
When using, as shown in Figure 1, with being immersed in the culture dish 1 of basilar membrane 2, the attached cell that is used for observation in basilar membrane 2 growths, with the mid-way of microscope alignment basilar membrane 2, start drive-motor 71 then, just can carry out dynamic bidirectional stretching original position online observation.
The above only is preferred embodiment of the present invention, is not limited to the present invention, and for a person skilled in the art, the present invention can have various changes and variation.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. the cell biological mechanical loading unit of a dynamic bidirectional stretching original position online observation is characterized in that, comprising:
Basilar membrane, growth is used for the attached cell of observation;
Retaining clip, described retaining clip are 2, respectively the both sides of clamping basilar membrane;
The displacement sway brace, described displacement sway brace is 2, is used for supporting described retaining clip respectively, described retaining clip is removably set on the guide rail;
Driving part, the opposite direction of doing symmetry at described guide rail for the described displacement sway brace of driving moves, so that described basilar membrane is carried out two-way stretch.
2. device as claimed in claim 1 is characterized in that, described driving part comprises: drive-motor, bending and stretching arm, deflecting wheel chute, deflecting wheel and electric machine support; Drive-motor is arranged on the electric machine support, and deflecting wheel is arranged on the clutch end of described drive-motor; One end of bending and stretching arm is rotatable to be arranged on the described displacement sway brace, and the other end is rotatable to be arranged on the described deflecting wheel chute; Described deflecting wheel is embedded in described deflecting wheel chute.
3. device as claimed in claim 2 is characterized in that, one of them is arranged on described retaining clip on the described displacement sway brace by the microspur adjusting slider.
4. device as claimed in claim 3 is characterized in that, also comprises the microspur adjusting knob of regulating the displacement of described microspur adjusting slider.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093826.2A CN103184144B (en) | 2013-03-22 | 2013-03-22 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093826.2A CN103184144B (en) | 2013-03-22 | 2013-03-22 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
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| CN103184144A true CN103184144A (en) | 2013-07-03 |
| CN103184144B CN103184144B (en) | 2015-04-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867888A (en) * | 2017-03-20 | 2017-06-20 | 北京理工大学 | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time |
| CN109468225A (en) * | 2018-11-12 | 2019-03-15 | 太原理工大学 | A kind of cell mechanical tensioning devices of real time data feedback |
| CN111117883A (en) * | 2019-03-28 | 2020-05-08 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1932510A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Cell tensile loader |
| CN1932511A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Sinusoidal tensile cell loader |
| CN101092595A (en) * | 2006-06-23 | 2007-12-26 | 上海交通大学医学院附属第九人民医院 | Experimental apparatus for loading cell through digital controlled mechanical strain |
| CN200999244Y (en) * | 2007-01-09 | 2008-01-02 | 重庆大学 | Cell substrate uniaxial drawing apparatus |
| CN201008641Y (en) * | 2006-12-31 | 2008-01-23 | 林恩赐 | Massager of massage chair |
| CN101298592A (en) * | 2008-06-16 | 2008-11-05 | 重庆大学 | Cell three-dimensional mechanical loading unit |
| CN201261787Y (en) * | 2008-06-13 | 2009-06-24 | 国家纳米科学中心 | Cell cultivation apparatus for exerting mechanical stimulation on cell |
| CN101649291A (en) * | 2009-08-25 | 2010-02-17 | 四川大学 | Extension and compression device of multi-unit cells |
| CN201737929U (en) * | 2010-07-09 | 2011-02-09 | 东南大学 | Precision visualization cell stretching device under environment simulating inner environment of human body |
-
2013
- 2013-03-22 CN CN201310093826.2A patent/CN103184144B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101092595A (en) * | 2006-06-23 | 2007-12-26 | 上海交通大学医学院附属第九人民医院 | Experimental apparatus for loading cell through digital controlled mechanical strain |
| CN1932510A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Cell tensile loader |
| CN1932511A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Sinusoidal tensile cell loader |
| CN201008641Y (en) * | 2006-12-31 | 2008-01-23 | 林恩赐 | Massager of massage chair |
| CN200999244Y (en) * | 2007-01-09 | 2008-01-02 | 重庆大学 | Cell substrate uniaxial drawing apparatus |
| CN201261787Y (en) * | 2008-06-13 | 2009-06-24 | 国家纳米科学中心 | Cell cultivation apparatus for exerting mechanical stimulation on cell |
| CN101298592A (en) * | 2008-06-16 | 2008-11-05 | 重庆大学 | Cell three-dimensional mechanical loading unit |
| CN101649291A (en) * | 2009-08-25 | 2010-02-17 | 四川大学 | Extension and compression device of multi-unit cells |
| CN201737929U (en) * | 2010-07-09 | 2011-02-09 | 东南大学 | Precision visualization cell stretching device under environment simulating inner environment of human body |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867888A (en) * | 2017-03-20 | 2017-06-20 | 北京理工大学 | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time |
| CN109468225A (en) * | 2018-11-12 | 2019-03-15 | 太原理工大学 | A kind of cell mechanical tensioning devices of real time data feedback |
| CN111117883A (en) * | 2019-03-28 | 2020-05-08 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
| CN111117883B (en) * | 2019-03-28 | 2020-12-25 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
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