CN201908092U - Cell cyclic compression and tension device - Google Patents
Cell cyclic compression and tension device Download PDFInfo
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- CN201908092U CN201908092U CN2010205959816U CN201020595981U CN201908092U CN 201908092 U CN201908092 U CN 201908092U CN 2010205959816 U CN2010205959816 U CN 2010205959816U CN 201020595981 U CN201020595981 U CN 201020595981U CN 201908092 U CN201908092 U CN 201908092U
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- cell compression
- stretch device
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Abstract
The utility model discloses a cell cyclic compression and tension device, comprising a cell culture plate (1), a supporting frame (2), an ejection rod (3) and a linear stepping motor (4), wherein the cell culture plate (1) is fixed on an upper plate (21) of the supporting frame (2); the center of the upper plate (21) is provided with a circular hole (211); the linear stepping motor (4) is fixed below the upper plate (21) and connected with the ejection rod (3); and the upper end of the ejection rod (3) has a domelike structure, is positioned right below the circular hole (211) of the upper plate of the supporting frame, and corresponds to the bottom part of the cell culture plate (1). The device can apply a compression stress or a tension stress to a cell under same conditions, and can control the magnitude and frequency of applied stress.
Description
Technical field
The utility model relates to a kind of periodicity cell compression stretch device.
Background technology
For a long time, the influence of mechanical stimulus pair cell biological behaviour is the important directions of domestic and international RESEARCH ON CELL-BIOLOGY always, scholars use the stressed microenvironment of the afterburning mode analogue body inner cell of various cell in vitro, attempt to study various interaction of molecules mechanism in the cell that mechanical stimulus causes, be subjected to the mechanism of reconstructing by metabolism after the mechanical force to illustrate osseous tissue.In Orthodontics, the principle that tooth moves is mechanical force and causes the alveolar bone tissue reconstruction.Frontal resorption on the pressure side, tension side alveolar bone deposition, thus the tooth of dislocation is moved.Up to now, purely based on these two corresponding notions of just abnormal clinical " compression " and " stretching ", and the research that loads of pair cell under the same conditions is very rare.Only having invented the four point bending cell mechanics loader pair cell in calendar year 2001 by people such as Zhou Zheng compresses or stretching.At first make cell attachment grow in certain thickness rectangle duroplasts and cultivate sheet, will cultivate sheet as simply supported beam, bear vertical to uniformly distributed load.When the cultivation sheet is subjected to top-down load, cultivates sheet upper surface cell and be subjected to unidirectional force of compression; When being subjected to load from bottom to top, cultivating sheet upper surface cell and be subjected to unidirectional distraction force, as accompanying drawing 1.This afterburning mode is because cultivation sheet material is a duroplasts, and its maximum tension intensity is little, and the mechanical stress power value that pair cell applies has been subjected to certain limitation.In addition, unidirectional stretching can make cell be compressed power with the perpendicular direction of stretching, in like manner unidirectional compression, can make cell being subjected to distraction force, as accompanying drawing 2 (arrow is outwards represented distraction force, and arrow is inwardly represented force of compression) with compressing perpendicular direction.Therefore, be difficult to that clear to differentiate the bulk property that cell is subjected to mechanical load power be that stress under compression still is a stretching stress.Pair cell under the same conditions compresses and the afterburning instrument of the cell of stretching owing to lack, and rare so far pair cell by compression and be subjected to the comparative study of molecular biology response difference after the stretching.
The utility model content
Technical problem to be solved in the utility model provides a kind of compression stretch device, and this device pair cell under the same conditions applies stress under compression or stretching stress, accurately controls stress application size and frequency.Thereby set up the biology comparison model after cell is subjected to distraction force and force of compression, reach the simulation mouth cavity orthodontic clinical in, alveolar bone one side cell pressurized, a side cell is subjected to the phenomenon of stretching.
For solving the problems of the technologies described above, technical scheme provided by the utility model is:
The utility model provides a kind of periodicity cell compression stretch device, and this device comprises Tissue Culture Dish, bracing frame, push rod and linear stepping motor; Described Tissue Culture Dish is fixed on the top plate of bracing frame, these top plate central authorities are provided with circular hole, described linear stepping motor is fixed in the below of top plate, and be connected with push rod, the upper end of this push rod is a dome shape structure, its be positioned at bracing frame top plate circular hole under, and corresponding with the bottom of Tissue Culture Dish.
Described Tissue Culture Dish comprises Tissue Culture Dish body, transparent elastic film and the set collar of removing the bottom surface, and this removes the Tissue Culture Dish bottom external parcel transparent elastic film of bottom surface, at the outside sheathed set collar of this transparent elastic film.
Further, described Tissue Culture Dish body and transparent elastic film junction are provided with gasket ring, and this gasket ring is used to eliminate the region of stress concentration at ware feather edge place, and being called again eliminates stress concentrates ring.
Further, the thickness of described transparent elastic film is 0.2mm～0.5mm, can select rubber film for use, for example silicon rubber film.Cell can be attached to growing on this Elastic Film.
Further, the below of the top plate of support frame as described above is provided with lower plywood, and described linear stepping motor is fixed on this lower plywood.
Further, this device also comprises the controller that links to each other with linear stepping motor, and described controller can be the micro computer motion controller.
Periodicity cell described in the utility model compression stretch device groundwork principle is: elasticity homogeneous film in its elastic deformation scope stressed after, the stress and the deformation that are produced are linear.Deformation takes place at the bottom of the Elastic Film ware, can carry out stretching or compression by pair cell: make cell adherent growth when film is relaxation state at the bottom of the ware, at the bottom of cell is attached to ware fully on the film, push rod rises, film at the bottom of the dome shape structure jack-up ware of push rod upper end, then film is subjected to stretching at the bottom of the ware, and this moment, cell was subjected to distraction force, as accompanying drawing 3A (direction of arrow representative is subjected to force direction); Push rod rises earlier, film at the bottom of the dome shape structure jack-up ware of push rod upper end, film is the stretching state at the bottom of the ware, make cell adherent growth on film at the bottom of the ware, when cell was attached on the film fully, push rod descended, and then film is loosened by the stretching state at the bottom of the ware, this moment, cell was compressed power, as accompanying drawing 3B (direction of arrow representative is subjected to force direction).
Advantage of the present utility model: the utility model pair cell under the same conditions carries out stress loading size and controlled multidirectional compression or the stretching of frequency.Thereby set up the biology comparison model after cell is subjected to distraction force and force of compression, reach the simulation mouth cavity orthodontic clinical in, alveolar bone one side cell pressurized, a side cell is subjected to the phenomenon of stretching.
Description of drawings:
Below in conjunction with the drawings and specific embodiments the utility model is described in further detail.
Fig. 1 cultivates the synoptic diagram that compresses on the sheet with stretching for cell in the prior art at the rectangle duroplasts;
Fig. 2 cultivates the synoptic diagram that is compressed power and distraction force on the sheet for cell in the prior art at the rectangle duroplasts;
Fig. 3 A is that cell carries out stretching and is subjected to the synoptic diagram of distraction force on Elastic Film of the present utility model;
Fig. 3 B is cell compresses and be compressed power on an Elastic Film of the present utility model synoptic diagram;
Fig. 4 is the structural representation of Tissue Culture Dish;
Fig. 5 is the periodically structural representation of cell compression stretch device of the utility model.
Embodiment:
Embodiment 1
Shown in Figure 4 and 5, periodicity cell compression stretch device described in the utility model, this device comprises Tissue Culture Dish 1, bracing frame 2, push rod 3 and linear stepping motor 4; Described Tissue Culture Dish 1 is fixed on the top plate 21 of bracing frame 2, these top plate 21 central authorities are provided with circular hole 211, described linear stepping motor 4 is fixed on the lower plywood 22 of bracing frame 2, and be connected with push rod 3, the upper end of this push rod 3 is a dome shape structure, its be positioned at bracing frame top plate circular hole 211 under, and corresponding with the bottom of Tissue Culture Dish 1, this linear stepping motor (4) links to each other with controller (5).
Described Tissue Culture Dish 1 is made up of Tissue Culture Dish body 11, transparent elastic film 12, set collar 13 and the gasket ring 14 of removing the bottom surface, this removes the Tissue Culture Dish body 11 bottom external parcel transparent elastic films 12 of bottom surface, at the outside sheathed set collar of this transparent elastic film (12) (13), described Tissue Culture Dish (11) is provided with gasket ring (14) with transparent elastic film (12) junction.
Described transparent elastic film is a silicon rubber film, and its thickness is 0.2mm～0.5mm.
(Haydon Kerk Motion Solutions, USA) the drive push rod is finished the reinforcing operation to film to described linear stepping motor.The linear stepping motor step-length is 0.012192mm, and stroke is 0-12mm, and controller is adjustment stroke length voluntarily, and sports rule is (0-0.5mm, 0-1mm ..., 0-8.5mm, 0-9mm), in these scopes, select interval cyclic motion back and forth arbitrarily.Motor thrust is more than the 8N, can circulate up and down 60 times in one minute.Sustainable work is more than 20 hours.
Described controller adopts the SC100 programmable logic controller, by the program setting, finishes the accurately control automatically to the stepper-motor working order.
One, push rod displacement and film deformation relationship
In thin flexible film deformation range at the bottom of the ware, diaphragm deformation was that homogeneous changes when push rod moved up and down, and was attached to cell on the film this moment for evenly stressed.Push rod displacement and diaphragm deformation relationship can be used the Solidworks three-dimensional software, and (Dassault Systemes, France) drawing calculates table 1.
Displacement of table 1 push rod and diaphragm deformation relationship table (length variations 0.1%=1000microstrain)
Two, the stressed relation of push rod displacement and cell
Get 3 flexible sheets arbitrarily, (Instron 3367, and USA) to its test, the stress-strain curve of 3 flexible sheets in the elastic deformation scope all is the straight line of slope unanimity to use the electronic universal experiment instrument.
Can converse push rod displacement and the stressed relation of cell according to above-mentioned push rod displacement and diaphragm deformation relationship again, as table 2.
The stressed relation of displacement of table 2 push rod and cell
For simulate just abnormal clinical in, stressed tooth one side alveolar bone inner cell is by stretching, opposite side alveolar bone inner cell is compressed, and adopts SC100 microcomputerized control cell cycle property augmenter that the scleroblast in the culture dish is compressed or stretching in this experiment, and concrete afterburning mode is as follows:
A) control group: not afterburning cell
B) experimental group 1: cell is subjected to continue force of compression 800microstrain one hour, and the power value strengthened 1600microstrain one hour, 3400microstrain, 5200microstrain one hour, 7000microstrain one hour.
C) experimental group 2: cell is subjected to continue distraction force 800microstrain one hour, and the power value strengthened 1600microstrain one hour, 3400microstrain, 5200microstrain one hour, 7000microstrain one hour.
It is as follows to work out the instruction of SC100 micro computer according to the afterburning mode of cell:
Experimental group 1: the instantaneous rising 1mm of following programmed instruction push rod.
00?lspeed?800
01?aspeed?5000
02?hspeed?164
03?p-move-82
04?c-done
05?end
Experimental group 2: the instantaneous decline 1mm of following programmed instruction push rod.
00?lspeed?800
01?aspeed?5000
02?hspeed?164
03?p-move?82
04?c-done
05?end
After the force of compression and distraction force 5h that cell is subjected to continue to increase, observe discovery down by laser confocal microscope, different experiments group cellular form and skeleton change and have notable difference, show that scleroblast receives mechanical signal after, can distinguish the difference of different mechanical signals.Be subjected to the stretching group, fine long before cellular form is more stressed, the microfilament form is more clear obviously in the cell, arranges to be the straight state that led; Behind the force of compression 5h that cell is subjected to continue to increase, shrinkage before cellular form is more stressed, microfilament is fuzzy in the cell, and arrangement disorder is unclear.Scleroblast is subjected to stretching and force of compression under same experimental conditions after, the reconstruction of cytoskeleton exists notable difference.
Obviously, the foregoing description of the present utility model only is for the utility model example clearly is described, and is not to be qualification to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here can't give exhaustive to all embodiments.Everyly belong to the row that conspicuous variation that the technical solution of the utility model extends out or change still are in protection domain of the present utility model.
Claims (10)
1. a periodicity cell compression stretch device is characterized in that this device comprises Tissue Culture Dish (1), bracing frame (2), push rod (3) and linear stepping motor (4); Described Tissue Culture Dish (1) is fixed on the top plate (21) of bracing frame (2), this top plate (21) central authorities are provided with circular hole (211), described linear stepping motor (4) is fixed in the below of top plate (21), and be connected with push rod (3), the upper end of this push rod (3) is a dome shape structure, its be positioned at bracing frame top plate circular hole (211) under, and corresponding with the bottom of Tissue Culture Dish (1).
2. periodicity cell compression stretch device according to claim 1, it is characterized in that, described Tissue Culture Dish (1) comprises Tissue Culture Dish body (11), transparent elastic film (12) and the set collar (13) of removing the bottom surface, this removes Tissue Culture Dish body (11) the bottom external parcel transparent elastic film (12) of bottom surface, at the outside sheathed set collar of this transparent elastic film (12) (13).
3. periodicity cell compression stretch device according to claim 1 and 2 is characterized in that described Tissue Culture Dish body (11) is provided with gasket ring (14) with transparent elastic film (12) junction.
4. periodicity cell compression stretch device according to claim 3 is characterized in that described transparent elastic film is a rubber film.
5. periodicity cell compression stretch device according to claim 3 is characterized in that the thickness of described transparent elastic film is 0.2mm～0.5mm.
6. periodicity cell compression stretch device according to claim 3 is characterized in that the below of the top plate (21) of support frame as described above (2) is provided with lower plywood (22), and described linear stepping motor (4) is fixed on this lower plywood (22).
7. according to claim 1 or the described periodicity cell compression of 2 arbitrary claims stretch device, it is characterized in that this device also comprises the controller (5) that links to each other with linear stepping motor (4).
8. periodicity cell compression stretch device according to claim 7 is characterized in that described controller is the micro computer motion controller.
9. periodicity cell compression stretch device according to claim 3 is characterized in that this device also comprises the controller (5) that links to each other with linear stepping motor (4).
10. periodicity cell compression stretch device according to claim 9 is characterized in that described controller is the micro computer motion controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205959816U CN201908092U (en) | 2010-11-08 | 2010-11-08 | Cell cyclic compression and tension device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205959816U CN201908092U (en) | 2010-11-08 | 2010-11-08 | Cell cyclic compression and tension device |
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| CN201908092U true CN201908092U (en) | 2011-07-27 |
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| CN2010205959816U Expired - Fee Related CN201908092U (en) | 2010-11-08 | 2010-11-08 | Cell cyclic compression and tension device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110438004A (en) * | 2019-09-04 | 2019-11-12 | 北京航空航天大学 | Flat flow chamber that is a kind of while applying static stretch power and Osima jacoti, Osima excavata |
| CN110577895A (en) * | 2019-10-14 | 2019-12-17 | 东华大学 | dynamic cell culture method and culture device for simulating in-vivo dynamic environment |
| CN111117883A (en) * | 2019-03-28 | 2020-05-08 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
| CN111545258A (en) * | 2020-04-26 | 2020-08-18 | 复旦大学 | Micro-fluidic chip capable of providing compression deformation and preparation method and application thereof |
| CN111718835A (en) * | 2020-06-12 | 2020-09-29 | 西安工业大学 | Cell tissue mechanics analogue means |
| JP2021052659A (en) * | 2019-09-30 | 2021-04-08 | テルモ株式会社 | Container for processing sheet-like cell culture with ridge |
-
2010
- 2010-11-08 CN CN2010205959816U patent/CN201908092U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111117883A (en) * | 2019-03-28 | 2020-05-08 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
| CN111117883B (en) * | 2019-03-28 | 2020-12-25 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
| CN110438004A (en) * | 2019-09-04 | 2019-11-12 | 北京航空航天大学 | Flat flow chamber that is a kind of while applying static stretch power and Osima jacoti, Osima excavata |
| JP2021052659A (en) * | 2019-09-30 | 2021-04-08 | テルモ株式会社 | Container for processing sheet-like cell culture with ridge |
| JP7330044B2 (en) | 2019-09-30 | 2023-08-21 | テルモ株式会社 | Vessel for treatment of sheet-like cell culture with protrusions |
| CN110577895A (en) * | 2019-10-14 | 2019-12-17 | 东华大学 | dynamic cell culture method and culture device for simulating in-vivo dynamic environment |
| CN111545258A (en) * | 2020-04-26 | 2020-08-18 | 复旦大学 | Micro-fluidic chip capable of providing compression deformation and preparation method and application thereof |
| CN111718835A (en) * | 2020-06-12 | 2020-09-29 | 西安工业大学 | Cell tissue mechanics analogue means |
| CN111718835B (en) * | 2020-06-12 | 2023-05-26 | 西安工业大学 | Cell tissue mechanics simulator |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110727 Termination date: 20121108 |