CN111718835A - Cell tissue mechanics analogue means - Google Patents
Cell tissue mechanics analogue means Download PDFInfo
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- CN111718835A CN111718835A CN202010535902.0A CN202010535902A CN111718835A CN 111718835 A CN111718835 A CN 111718835A CN 202010535902 A CN202010535902 A CN 202010535902A CN 111718835 A CN111718835 A CN 111718835A
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- cell tissue
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- injection pump
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- silicon rubber
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Abstract
The invention belongs to the technical field of application of flexible materials, particularly relates to the field of application of a soft driver, and particularly relates to a cell tissue mechanics simulation device. The device simulates the movement of cell tissues and provides effective and real mechanical environment conditions for in-vitro organ culture and experiments of medicaments in tissues and organs. The technical scheme of the invention comprises an injection pump, an injection pump controller and a soft driver, wherein the soft driver is sequentially connected with the injection pump and the injection pump controller; the soft driver structure comprises a cell tissue layer, a film, a supporting column, a silicone rubber layer, a cavity and a non-extensible material layer; two support columns are arranged and symmetrically arranged on the silicon rubber layer; the cell tissue layer is attached to the thin film, two ends of the thin film are fixedly arranged between the two supporting columns, and the inextensible material layer is U-shaped and arranged at the bottom of the silicon rubber layer; the supporting column and the silicon rubber layer are both formed by injection molding of silicon rubber; the film is an elastomer.
Description
The technical field is as follows:
the invention belongs to the technical field of application of flexible materials, particularly relates to the field of application of a soft driver, and particularly relates to a cell tissue mechanics simulation device.
Background art:
at present, researches aiming at the physical environment of tissue and organ growth and the construction thereof mainly focus on the mechanical action mechanism and the construction of the mechanical environment, but most of scholars at home and abroad still focus on the physical function of organs, and less mechanical researches on cell tissues are carried out. However, with the development of flexible materials and soft body drivers in recent years, research on organoid technology has been conducted from the organ layer to the cellular tissue layer. Most of the currently researched cell tissue mechanics simulation devices are made of rigid materials, are poor in biocompatibility and are single in researched mechanical property.
The invention content is as follows:
in view of the above, the present invention provides a device for simulating cell tissue mechanics, which simulates the movement of cell tissue and provides effective and real mechanical environmental conditions for in vitro organ culture and drug experiments in tissues and organs.
In order to solve the problems in the prior art, the technical scheme of the invention is that the cell tissue mechanics simulation device comprises an injection pump and an injection pump controller, and is characterized in that: the injection pump is characterized by also comprising a soft driver, wherein the soft driver is sequentially connected with the injection pump and the injection pump controller;
the soft driver structure comprises a cell tissue layer, a film, a supporting column, a silicone rubber layer, a cavity and a non-extensible material layer; two support columns are arranged and symmetrically arranged on the silicon rubber layer; the cell tissue layer is attached to the thin film, two ends of the thin film are fixedly arranged between the two supporting columns, and the inextensible material layer is U-shaped and arranged at the bottom of the silicon rubber layer;
the supporting column and the silicon rubber layer are both formed by injection molding of silicon rubber; the film is an elastomer.
Further, the film and the support column are glued and fixed.
Further, the film is a silica gel film.
Furthermore, the material of the inextensible material layer is fiber reinforced silica gel.
Compared with the prior art, the invention has the following advantages:
1) the soft driver in the invention is a structural layer with different rigidity, the upper layer is silicon rubber with better flexibility, the lower layer is fiber reinforced silica gel with higher rigidity, the structural design can inhibit the ineffective deformation of the cavity in the left, right and lower directions, and the change of nonlinear force is reduced, so that the upper direction achieves the best mechanical property;
2) the supporting column on the soft driver can support the film and transfer acting force; when the film is in a pre-tightening state, the pre-tightening force is loaded, so that the stretching and the compression of cell tissues can be simultaneously researched under the same condition;
3) the software driver adopts a layered structure, so that the processing difficulty and the manufacturing cost are reduced;
4) the fiber reinforced silica gel and the silica gel layer of the soft driver are made of similar materials, and when the fiber reinforced silica gel and the silica gel layer are bonded, the sealing performance of the cavity is good;
5) the device has low cost, easy operation and good biocompatibility, and can accurately simulate the movement of cell tissues; the device is suitable for the research of the cell tissue mechanical environment in the external environment, and realizes the stretching and compressing effects of different degrees of cell tissue layers by controlling the pressure in the cavity through the injection pump controller.
Description of the drawings:
FIG. 1 is a schematic diagram of a device for simulating cell tissue mechanics according to the present invention;
FIG. 2 is a structural diagram of the soft body driver of the present invention under a constant pressure (standard atmospheric pressure);
FIG. 3 is an operational view of the soft body driver of the present invention under compression;
FIG. 4 is the operation diagram of the soft driver of the present invention under reduced pressure;
FIG. 5 is a three-dimensional cross-sectional view of the soft drive of the present invention;
description of reference numerals: 1-cell tissue layer, 2-membrane, 3-support column, 4-silicon rubber layer, 5-cavity, 6-inextensible material layer, 7-soft driver, 8-injection pump and 9-injection pump controller.
The specific implementation mode is as follows:
in order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The present embodiment provides a device for simulating cell tissue mechanics, as shown in fig. 1 and 5, comprising a soft body driver 7, an injection pump 8 and an injection pump controller 9, wherein the soft body driver 7 is connected to the injection pump 8 and the injection pump controller 9 in sequence; the injection pump controller 9 is mainly used for controlling the injection pump, controlling the fluid in the cavity 5 by the injection pump (wherein the fluid in the cavity 5 is gaseous), and changing the shape and volume of the cavity, so that the force magnitude and direction on the supporting column 3 are changed.
The soft driver 7 structure comprises a cell tissue layer 1, a membrane 2, a support column 3, a silicone rubber layer 4, a cavity 5 and a non-extensible material layer 6; two support columns 3 are arranged on the silicone rubber layer 4 symmetrically; the cell tissue layer 1 is attached to the thin film 2, two ends of the thin film 2 are fixedly glued between the two support columns 3, and the thin film is fixed on the support columns in a silica gel bonding mode after being stretched to generate pre-tightening; the inextensible material layer 6 is U-shaped and is arranged at the bottom of the silicone rubber layer 4,
the film 3 is an elastic body, generally adopts a medical silica gel film, plays a role in a carrier and a transmission action of cell tissues, and the cell tissues are attached to the film; the thin film 2 passes through the direction of the force on the supporting columns 3, and the thin film 2 is subjected to stretching and compressing motions, and the cell tissue is stretched and compressed along with the force applied to the thin film (cell growth is generally called 'adherent growth').
The supporting column 3 and the silicon rubber layer 4 are both formed by injection molding of silicon rubber;
the thin film 2 and the support columns 3 stretch or compress the cell tissue layer;
the silicone rubber layer 4 is an extensible material, and provides forces in different directions for the support body through the flexibility of the silicone rubber layer;
the inextensible material layer 6 is made of fiber-reinforced silicone rubber for enhancing the rigidity of the silicone rubber.
The pressure in the cavity in the U-shaped inextensible material layer 6 is air pressure and is provided by an injection pump 8, the air pressure input by the injection pump is controlled by an injection pump controller 9, and the pressure is different, so that the deformation above the cavity is different. The inextensible material layer 6 has high rigidity, and is mainly used for preventing unnecessary nonlinear mechanics caused by excessive deformation of the left, right and lower surfaces of the cavity 5 and increasing the research difficulty.
The implementation mode of the soft driver under constant pressure, pressurization and depressurization of the invention is as follows:
as shown in fig. 2, when the air pressure in the cavity 5 is not changed, i.e. the injection pump 8 does not input or output the air pressure into or out of the cavity, the volume of the cavity 5 is not changed, the surface S is not changed, but the thin film 2 is loaded with a certain stretching force (pretightening force), i.e. the thin film is in a "pretightening" state; the cell tissue is cultured on the film, is free from external force and is in a 'gentle' state, and the length of the cell tissue layer is S0。
The pressurized soft driver, as shown in fig. 3, wherein the air pressure in the cavity is increased, that is, the injection pump 8 delivers air pressure into the cavity 5, the volume of the cavity 5 is increased, the S-face is raised significantly, the support pillar 3 is subjected to an outward force F, the stretching force on the membrane is increased, the cell tissue layer 1 is subjected to stretching forces (initial pre-tightening force and post-applied stretching force) and is in a "stretched" state, and the length of the cell tissue layer is S1。
The soft driver under reduced pressure, as shown in fig. 4, has a cavity with reduced air pressure, i.e. the syringe pump draws air pressure from the cavity, the cavity has a reduced volume, and the S-face is recessed significantly. The supporting column 3 is applied with inward force F, the stretching force on the membrane is reduced, the cell tissue layer 1 is applied with compression force and is in a 'compression' state, and the length of the cell tissue layer is S2。
S in FIGS. 2 to 41>S0>S2。
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention.
Claims (4)
1. A cell tissue mechanics simulation device, comprising a syringe pump (8) and a syringe pump controller (9), characterized in that: the injection pump is characterized by further comprising a soft driver (7), wherein the soft driver (7) is sequentially connected with an injection pump (8) and an injection pump controller (9);
the soft driver (7) structure comprises a cell tissue layer (1), a membrane (2), a support column (3), a silicone rubber layer (4), a cavity (5) and a non-extensible material layer (6); two support columns (3) are arranged on the silicon rubber layer (4) symmetrically; the cell tissue layer (1) is attached to the thin film (2), two ends of the thin film (2) are fixedly arranged between the two supporting columns (3), and the inextensible material layer (6) is U-shaped and is arranged at the bottom of the silicone rubber layer (4);
the supporting column (3) and the silicon rubber layer (4) are both formed by injection molding of silicon rubber; the film (2) is an elastomer.
2. A cell tissue mechanics simulation device according to claim 1, wherein: the film (2) and the supporting column (3) are glued and fixed.
3. A cell tissue mechanics simulation device according to claim 1 or 2, wherein: the film (2) is a silica gel film.
4. A cell tissue mechanics simulation device according to claim 3, wherein: the material of the inextensible material layer (6) is fiber reinforced silica gel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010535902.0A CN111718835B (en) | 2020-06-12 | 2020-06-12 | Cell tissue mechanics simulator |
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| CN202010535902.0A CN111718835B (en) | 2020-06-12 | 2020-06-12 | Cell tissue mechanics simulator |
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| CN111718835A true CN111718835A (en) | 2020-09-29 |
| CN111718835B CN111718835B (en) | 2023-05-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113073054A (en) * | 2021-04-02 | 2021-07-06 | 陕西科技大学 | Cell culture device capable of providing cyclic tensile stress stimulation and manufacturing method |
| CN113214991A (en) * | 2021-05-28 | 2021-08-06 | 西安交通大学 | Cell culture device for simulating cell mechanics microenvironment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113073054A (en) * | 2021-04-02 | 2021-07-06 | 陕西科技大学 | Cell culture device capable of providing cyclic tensile stress stimulation and manufacturing method |
| CN113214991A (en) * | 2021-05-28 | 2021-08-06 | 西安交通大学 | Cell culture device for simulating cell mechanics microenvironment |
| CN113214991B (en) * | 2021-05-28 | 2022-12-09 | 西安交通大学 | Cell culture device for simulating cell mechanics microenvironment |
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| CN111718835B (en) | 2023-05-26 |
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