CN111718835B - Cell tissue mechanics simulator - Google Patents
Cell tissue mechanics simulator Download PDFInfo
- Publication number
- CN111718835B CN111718835B CN202010535902.0A CN202010535902A CN111718835B CN 111718835 B CN111718835 B CN 111718835B CN 202010535902 A CN202010535902 A CN 202010535902A CN 111718835 B CN111718835 B CN 111718835B
- Authority
- CN
- China
- Prior art keywords
- film
- silicon rubber
- layer
- cell tissue
- syringe pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to the technical field of application of flexible materials, in particular to the field of application of a software driver, and particularly relates to a cell tissue mechanics simulation device. The device simulates the movement of cell tissues and provides effective and real mechanical environment conditions for in-vitro organ culture and experiments of medicines in tissues and organs. The technical scheme of the invention comprises a syringe pump, a syringe pump controller and a software driver, wherein the software driver is sequentially connected with the syringe pump and the syringe pump controller; the soft driver structure comprises a cell tissue layer, a film, a support column, a silicon rubber layer, a cavity and a non-extensible material layer; the number of the supporting columns is two, and the supporting columns are symmetrically arranged on the silicon rubber layer; the cell tissue layer is attached to the film, two ends of the film are fixedly arranged between the two support columns, and the inextensible material layer is U-shaped and is arranged at the bottom of the silicon rubber layer; the support columns and the silicon rubber layers are formed by injection molding of silicon rubber; the film is an elastomer.
Description
Technical field:
the invention belongs to the technical field of application of flexible materials, in particular to the field of application of a software driver, and particularly relates to a cell tissue mechanics simulation device.
The background technology is as follows:
at present, research on physical environment of tissue and organ growth and construction thereof is mainly focused on mechanical action mechanism and mechanical environment construction, but students at home and abroad research is more or focused on physical functions of organs, and mechanical research on cell tissues is less. However, with the recent development of flexible materials and soft drivers, organoid technology has been studied deeply from the organ level toward the cellular tissue level. Most of the cell tissue mechanics simulation devices studied at present are made of rigid materials, have poor biocompatibility and have single mechanical properties.
The invention comprises the following steps:
in view of this, the invention provides a cell tissue mechanics simulation device which simulates the movement of a cell tissue and provides effective and real mechanics environment conditions for in vitro organ culture and experiments of medicines in tissues and organs.
In order to solve the problems in the prior art, the technical scheme of the invention is that the cell tissue mechanics simulation device comprises a syringe pump and a syringe pump controller, and is characterized in that: the injection pump comprises a syringe pump controller, a syringe pump and a soft driver, wherein the syringe pump controller is connected with the syringe pump controller;
the soft driver structure comprises a cell tissue layer, a film, a support column, a silicon rubber layer, a cavity and a non-extensible material layer; the number of the supporting columns is two, and the supporting columns are symmetrically arranged on the silicon rubber layer; the cell tissue layer is attached to the film, two ends of the film are fixedly arranged between the two support columns, and the inextensible material layer is U-shaped and is arranged at the bottom of the silicon rubber layer;
the support columns and the silicon rubber layers are formed by injection molding of silicon rubber; the film is an elastomer.
Further, the film and the support column are glued and fixed.
Further, the film is a silica gel film.
Further, the material of the inextensible material layer is fiber reinforced silica gel.
Compared with the prior art, the invention has the following advantages:
1) The soft driver is a structural layer with different rigidities, the upper layer is silicon rubber with better flexibility, the lower layer is fiber reinforced silica gel with higher rigidity, and the structural design can inhibit the invalid deformation in the left, right and lower directions of the cavity, reduce the variation of nonlinear force and ensure that the upper direction achieves the optimal mechanical property;
2) The support column on the soft driver can support the film and transfer acting force; when the film is in a pre-tightening state, the pre-tightening force is loaded, so that the stretching and compression of the cell tissues can be studied simultaneously under the same condition;
3) The software driver adopts a layered structure, so that the processing technology difficulty is reduced, and the manufacturing cost is reduced;
4) The materials of the fiber reinforced silica gel and the silicon rubber layer of the soft driver are similar, and when the fiber reinforced silica gel and the silicon rubber layer are bonded, the cavity tightness is good;
5) The device has low cost, easy operation and good biocompatibility, and can accurately simulate the movement of cell tissues; the device is suitable for researching the mechanical environment of the cell tissues in an in-vitro environment, and realizes the stretching and compressing effects of the cell tissue layers in different degrees through controlling the pressure in the cavity by the injection pump controller.
Description of the drawings:
FIG. 1 is a schematic diagram of a cellular tissue mechanics simulation apparatus of the present invention;
FIG. 2 is a block diagram of a software driver of the present invention at constant pressure (normal atmospheric pressure);
FIG. 3 is a diagram of the operation of the software driver of the present invention under pressure;
FIG. 4 is a diagram of the operation of the software driver of the present invention at reduced pressure;
FIG. 5 is a three-dimensional cross-sectional view of a software driver of the present invention;
reference numerals illustrate: 1-cell tissue layer, 2-film, 3-support column, 4-silicone rubber layer, 5-cavity, 6-inextensible material layer, 7-software driver, 8-syringe pump, 9-syringe pump controller.
The specific embodiment is as follows:
the present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The embodiment provides a cell tissue mechanics simulation device, as shown in fig. 1 and 5, which comprises a soft driver 7, a syringe pump 8 and a syringe pump controller 9, wherein the soft driver 7 is sequentially connected with the syringe pump 8 and the syringe pump controller 9; the syringe pump controller 9 mainly controls the syringe pump, realizes the control of the syringe pump on the fluid in the cavity 5 (wherein the fluid in the cavity 5 is in a gaseous state), and realizes the change of the shape and the volume of the cavity, thereby realizing the change of the force and the direction on the support column 3.
The structure of the soft driver 7 comprises a cell tissue layer 1, a film 2, a support column 3, a silicon rubber layer 4, a cavity 5 and a non-extensible material layer 6; the number of the supporting columns 3 is two, and the two supporting columns are symmetrically arranged on the silicon rubber layer 4; the cell tissue layer 1 is attached to the film 2, two ends of the film 2 are fixedly arranged between two support columns 3 in a gluing way, and the film is fixed on the support columns in a silica gel bonding way after being stretched so as to generate pretension; the inextensible material layer 6 is U-shaped and is arranged at the bottom of the silicon rubber layer 4,
the film 3 is an elastomer, generally adopts a medical silica gel film, plays roles in carrying and transmitting acting force of cell tissues, and the cell tissues are attached to the film; the membrane 2 is stretched and compressed by the direction of the force on the support column 3, so that the cell tissue is stretched and compressed along with the stress of the membrane (the cell growth is generally "adherent growth").
The support column 3 and the silicon rubber layer 4 are formed by injection molding of silicon rubber;
the membrane 2 and the support column 3 stretch or compress the cell tissue layer;
the above-mentioned silicone rubber layer 4 is an extensible material which, by its flexible nature, provides the support with forces in different directions;
the non-extensible material layer 6 is made of fiber reinforced silica gel, so as to strengthen the rigidity of the silica gel.
The pressure in the cavity of the U-shaped inextensible material layer 6 is air pressure, the air pressure is provided by the injection pump 8, the air pressure input by the injection pump is controlled by the injection pump controller 9, the pressure is different, and the deformation above the cavity is different. The rigidity of the inextensible material layer 6 is high, mainly to prevent the excessive deformation of the left, right and lower three surfaces of the cavity 5 from generating unnecessary nonlinear mechanics, and increase the research difficulty.
The implementation of the constant pressure, pressurized, reduced pressure software driver of the present invention is as follows:
the soft driver is operated at constant pressure (standard atmospheric pressure) as shown in FIG. 2, wherein the air pressure in the cavity 5 is unchanged, i.e. the syringe pump 8 is not fed into the cavity orThe volume of the cavity 5 is not changed, the S surface is not changed, but the film 2 is loaded with a certain stretching force (pretightening force) at the moment, namely, the film is in a pretightening state; the cell tissue is cultured on the film and is not influenced by external force and is in a gentle state, and the length of the cell tissue layer is S 0 。
As shown in FIG. 3, the air pressure in the cavity is increased, that is, the injection pump 8 transmits air pressure into the cavity 5, the volume of the cavity 5 is increased, the S surface is obviously raised, the support column 3 is subjected to an outward force F, the stretching force on the film is increased, the cell tissue layer 1 is subjected to stretching force (initial pretightening force and post-applied stretching force) and is in a 'stretching' state, and the length of the cell tissue layer is S 1 。
The pressure of the air in the cavity of the soft driver under reduced pressure is reduced, as shown in fig. 4, namely the air is pumped out of the cavity by the injection pump, the volume of the cavity is reduced, and the S surface is obviously recessed. The support column 3 is subjected to an inward force F, at which time the tensile force on the membrane becomes smaller and the cell tissue layer 1 is subjected to a compressive force in a "compressed" state with a cell tissue layer length S 2 。
S in FIGS. 2-4 1 >S 0 >S 2 。
The foregoing description is only of the preferred embodiments of the present invention, and is not intended to limit the scope of the present invention.
Claims (4)
1. A cell tissue mechanics simulation device, comprising a syringe pump (8) and a syringe pump controller (9), characterized in that: the device also comprises a soft driver (7), wherein the soft driver (7) is sequentially connected with the injection pump (8) and the injection pump controller (9);
the soft driver (7) structure comprises a cell tissue layer (1), a film (2), a support column (3), a silicon rubber layer (4), a cavity (5) and a non-extensible material layer (6); the number of the supporting columns (3) is two, and the two supporting columns are symmetrically arranged on the silicon rubber layer (4); the cell tissue layer (1) is attached to the film (2), two ends of the film (2) are fixedly arranged between the two support columns (3), and the inextensible material layer (6) is U-shaped and is arranged at the bottom of the silicon rubber layer (4);
the support column (3) and the silicon rubber layer (4) are both formed by injection molding of silicon rubber; the film (2) is an elastomer.
2. A cellular tissue mechanics simulation apparatus according to claim 1, wherein: the film (2) and the support column (3) are glued and fixed.
3. A device according to claim 1 or 2, characterized in that: the film (2) is a silica gel film.
4. A cellular tissue mechanics simulation device according to claim 3, wherein: the inextensible material layer (6) is made of fiber reinforced silica gel.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010535902.0A CN111718835B (en) | 2020-06-12 | 2020-06-12 | Cell tissue mechanics simulator |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010535902.0A CN111718835B (en) | 2020-06-12 | 2020-06-12 | Cell tissue mechanics simulator |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111718835A CN111718835A (en) | 2020-09-29 |
CN111718835B true CN111718835B (en) | 2023-05-26 |
Family
ID=72566516
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010535902.0A Active CN111718835B (en) | 2020-06-12 | 2020-06-12 | Cell tissue mechanics simulator |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111718835B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113073054A (en) * | 2021-04-02 | 2021-07-06 | 陕西科技大学 | Cell culture device capable of providing cyclic tensile stress stimulation and manufacturing method |
CN113214991B (en) * | 2021-05-28 | 2022-12-09 | 西安交通大学 | Cell culture device for simulating cell mechanics microenvironment |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
CN1932511A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Sinusoidal tensile cell loader |
CN201908092U (en) * | 2010-11-08 | 2011-07-27 | 北京大学口腔医院 | Cell cyclic compression and tension device |
CN103805511A (en) * | 2014-02-18 | 2014-05-21 | 国家纳米科学中心 | Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective |
CN107988067A (en) * | 2017-11-08 | 2018-05-04 | 西安外事学院 | A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004094586A2 (en) * | 2003-04-18 | 2004-11-04 | Carnegie Mellon University | Three-dimentional, flexible cell growth substrate and related methods |
US7822457B2 (en) * | 2003-11-25 | 2010-10-26 | General Electric Company | Compression paddle membrane and tensioning apparatus for compressing tissue for medical imaging purposes |
-
2020
- 2020-06-12 CN CN202010535902.0A patent/CN111718835B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2818484Y (en) * | 2005-07-15 | 2006-09-20 | 中国人民解放军第四军医大学 | Cell tractive tension controller |
CN1932511A (en) * | 2006-09-22 | 2007-03-21 | 重庆大学 | Sinusoidal tensile cell loader |
CN201908092U (en) * | 2010-11-08 | 2011-07-27 | 北京大学口腔医院 | Cell cyclic compression and tension device |
CN103805511A (en) * | 2014-02-18 | 2014-05-21 | 国家纳米科学中心 | Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective |
CN107988067A (en) * | 2017-11-08 | 2018-05-04 | 西安外事学院 | A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape |
Non-Patent Citations (2)
Title |
---|
孙晓雷 ; 马剑雄 ; 马信龙 ; .成骨细胞应力加载方式及生物力学特性的研究进展.中国骨与关节外科.2010,(第03期),第250-254页. * |
张腊全 ; 张婷 ; 贾帅军 ; 刘建 ; 熊卓 ; .定向结构组织工程软骨支架的构建.航天医学与医学工程.2012,(第03期),第212-216页. * |
Also Published As
Publication number | Publication date |
---|---|
CN111718835A (en) | 2020-09-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111718835B (en) | Cell tissue mechanics simulator | |
US10385886B2 (en) | Soft actuators and soft actuating devices | |
Bar-Cohen et al. | Electroactive polymers as actuators | |
CN100581039C (en) | Gas-filled type dielectric elastomer hemi-spherical driver | |
CN110757434B (en) | Artificial muscle based on dielectric elastomer and intelligent fluid with adjustable rigidity and manufacturing method thereof | |
CN108263504A (en) | A kind of Pneumatic bionic software climbing robot | |
CN107972754A (en) | A kind of software climbing robot of marmem driving | |
CN102579018B (en) | Pulse condition acquiring contact device | |
CN102676446B (en) | Method and device for loading cell fluid stress on deformable curved surface and experimental platform | |
CN110497395B (en) | Bidirectional movement pneumatic flexible driver and working method thereof | |
CA2656876A1 (en) | Method of cultivating cell or tissue | |
WO2020013902A9 (en) | Refreshable tactile display using bistable electroactive polymer and deformable serpentine electrode | |
CN101603005A (en) | A kind of pair cell applies the cell culture apparatus of mechanical stimulation | |
CN107433611A (en) | A kind of soft drive unit of energy pre-storage | |
Herzig et al. | Model and validation of a highly extensible and tough actuator based on a ballooning membrane | |
CN111975807B (en) | Pneumatic control soft bionic manipulator | |
CN210650686U (en) | Flexible backbone of quadruped robot based on pneumatic muscle | |
CN111232076B (en) | Soft robot driver | |
CN111975808A (en) | Air control soft bionic mechanical finger | |
CN104046564A (en) | Physiological environment-imitating mechanical stimulation type biological reactor system | |
CN111347455A (en) | Flexible finger with online adjustable friction force | |
CN115998578A (en) | Vacuum enhancement type soft robot driving device and application thereof | |
CN208308875U (en) | A kind of cell culture apparatus for simulating massage effect | |
CN112137855B (en) | Rigidity-variable soft massage robot | |
CN116333882B (en) | Soft cell culture dish capable of realizing radial pulling and pressing and out-of-plane bending |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |