CN111718835B - Cell tissue mechanics simulator - Google Patents

Cell tissue mechanics simulator Download PDF

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Publication number
CN111718835B
CN111718835B CN202010535902.0A CN202010535902A CN111718835B CN 111718835 B CN111718835 B CN 111718835B CN 202010535902 A CN202010535902 A CN 202010535902A CN 111718835 B CN111718835 B CN 111718835B
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film
silicon rubber
layer
cell tissue
syringe pump
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CN111718835A (en
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李一青
曹岩
贾峰
吴军武
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Xian Technological University
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Xian Technological University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

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Abstract

The invention belongs to the technical field of application of flexible materials, in particular to the field of application of a software driver, and particularly relates to a cell tissue mechanics simulation device. The device simulates the movement of cell tissues and provides effective and real mechanical environment conditions for in-vitro organ culture and experiments of medicines in tissues and organs. The technical scheme of the invention comprises a syringe pump, a syringe pump controller and a software driver, wherein the software driver is sequentially connected with the syringe pump and the syringe pump controller; the soft driver structure comprises a cell tissue layer, a film, a support column, a silicon rubber layer, a cavity and a non-extensible material layer; the number of the supporting columns is two, and the supporting columns are symmetrically arranged on the silicon rubber layer; the cell tissue layer is attached to the film, two ends of the film are fixedly arranged between the two support columns, and the inextensible material layer is U-shaped and is arranged at the bottom of the silicon rubber layer; the support columns and the silicon rubber layers are formed by injection molding of silicon rubber; the film is an elastomer.

Description

Cell tissue mechanics simulator
Technical field:
the invention belongs to the technical field of application of flexible materials, in particular to the field of application of a software driver, and particularly relates to a cell tissue mechanics simulation device.
The background technology is as follows:
at present, research on physical environment of tissue and organ growth and construction thereof is mainly focused on mechanical action mechanism and mechanical environment construction, but students at home and abroad research is more or focused on physical functions of organs, and mechanical research on cell tissues is less. However, with the recent development of flexible materials and soft drivers, organoid technology has been studied deeply from the organ level toward the cellular tissue level. Most of the cell tissue mechanics simulation devices studied at present are made of rigid materials, have poor biocompatibility and have single mechanical properties.
The invention comprises the following steps:
in view of this, the invention provides a cell tissue mechanics simulation device which simulates the movement of a cell tissue and provides effective and real mechanics environment conditions for in vitro organ culture and experiments of medicines in tissues and organs.
In order to solve the problems in the prior art, the technical scheme of the invention is that the cell tissue mechanics simulation device comprises a syringe pump and a syringe pump controller, and is characterized in that: the injection pump comprises a syringe pump controller, a syringe pump and a soft driver, wherein the syringe pump controller is connected with the syringe pump controller;
the soft driver structure comprises a cell tissue layer, a film, a support column, a silicon rubber layer, a cavity and a non-extensible material layer; the number of the supporting columns is two, and the supporting columns are symmetrically arranged on the silicon rubber layer; the cell tissue layer is attached to the film, two ends of the film are fixedly arranged between the two support columns, and the inextensible material layer is U-shaped and is arranged at the bottom of the silicon rubber layer;
the support columns and the silicon rubber layers are formed by injection molding of silicon rubber; the film is an elastomer.
Further, the film and the support column are glued and fixed.
Further, the film is a silica gel film.
Further, the material of the inextensible material layer is fiber reinforced silica gel.
Compared with the prior art, the invention has the following advantages:
1) The soft driver is a structural layer with different rigidities, the upper layer is silicon rubber with better flexibility, the lower layer is fiber reinforced silica gel with higher rigidity, and the structural design can inhibit the invalid deformation in the left, right and lower directions of the cavity, reduce the variation of nonlinear force and ensure that the upper direction achieves the optimal mechanical property;
2) The support column on the soft driver can support the film and transfer acting force; when the film is in a pre-tightening state, the pre-tightening force is loaded, so that the stretching and compression of the cell tissues can be studied simultaneously under the same condition;
3) The software driver adopts a layered structure, so that the processing technology difficulty is reduced, and the manufacturing cost is reduced;
4) The materials of the fiber reinforced silica gel and the silicon rubber layer of the soft driver are similar, and when the fiber reinforced silica gel and the silicon rubber layer are bonded, the cavity tightness is good;
5) The device has low cost, easy operation and good biocompatibility, and can accurately simulate the movement of cell tissues; the device is suitable for researching the mechanical environment of the cell tissues in an in-vitro environment, and realizes the stretching and compressing effects of the cell tissue layers in different degrees through controlling the pressure in the cavity by the injection pump controller.
Description of the drawings:
FIG. 1 is a schematic diagram of a cellular tissue mechanics simulation apparatus of the present invention;
FIG. 2 is a block diagram of a software driver of the present invention at constant pressure (normal atmospheric pressure);
FIG. 3 is a diagram of the operation of the software driver of the present invention under pressure;
FIG. 4 is a diagram of the operation of the software driver of the present invention at reduced pressure;
FIG. 5 is a three-dimensional cross-sectional view of a software driver of the present invention;
reference numerals illustrate: 1-cell tissue layer, 2-film, 3-support column, 4-silicone rubber layer, 5-cavity, 6-inextensible material layer, 7-software driver, 8-syringe pump, 9-syringe pump controller.
The specific embodiment is as follows:
the present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The embodiment provides a cell tissue mechanics simulation device, as shown in fig. 1 and 5, which comprises a soft driver 7, a syringe pump 8 and a syringe pump controller 9, wherein the soft driver 7 is sequentially connected with the syringe pump 8 and the syringe pump controller 9; the syringe pump controller 9 mainly controls the syringe pump, realizes the control of the syringe pump on the fluid in the cavity 5 (wherein the fluid in the cavity 5 is in a gaseous state), and realizes the change of the shape and the volume of the cavity, thereby realizing the change of the force and the direction on the support column 3.
The structure of the soft driver 7 comprises a cell tissue layer 1, a film 2, a support column 3, a silicon rubber layer 4, a cavity 5 and a non-extensible material layer 6; the number of the supporting columns 3 is two, and the two supporting columns are symmetrically arranged on the silicon rubber layer 4; the cell tissue layer 1 is attached to the film 2, two ends of the film 2 are fixedly arranged between two support columns 3 in a gluing way, and the film is fixed on the support columns in a silica gel bonding way after being stretched so as to generate pretension; the inextensible material layer 6 is U-shaped and is arranged at the bottom of the silicon rubber layer 4,
the film 3 is an elastomer, generally adopts a medical silica gel film, plays roles in carrying and transmitting acting force of cell tissues, and the cell tissues are attached to the film; the membrane 2 is stretched and compressed by the direction of the force on the support column 3, so that the cell tissue is stretched and compressed along with the stress of the membrane (the cell growth is generally "adherent growth").
The support column 3 and the silicon rubber layer 4 are formed by injection molding of silicon rubber;
the membrane 2 and the support column 3 stretch or compress the cell tissue layer;
the above-mentioned silicone rubber layer 4 is an extensible material which, by its flexible nature, provides the support with forces in different directions;
the non-extensible material layer 6 is made of fiber reinforced silica gel, so as to strengthen the rigidity of the silica gel.
The pressure in the cavity of the U-shaped inextensible material layer 6 is air pressure, the air pressure is provided by the injection pump 8, the air pressure input by the injection pump is controlled by the injection pump controller 9, the pressure is different, and the deformation above the cavity is different. The rigidity of the inextensible material layer 6 is high, mainly to prevent the excessive deformation of the left, right and lower three surfaces of the cavity 5 from generating unnecessary nonlinear mechanics, and increase the research difficulty.
The implementation of the constant pressure, pressurized, reduced pressure software driver of the present invention is as follows:
the soft driver is operated at constant pressure (standard atmospheric pressure) as shown in FIG. 2, wherein the air pressure in the cavity 5 is unchanged, i.e. the syringe pump 8 is not fed into the cavity orThe volume of the cavity 5 is not changed, the S surface is not changed, but the film 2 is loaded with a certain stretching force (pretightening force) at the moment, namely, the film is in a pretightening state; the cell tissue is cultured on the film and is not influenced by external force and is in a gentle state, and the length of the cell tissue layer is S 0
As shown in FIG. 3, the air pressure in the cavity is increased, that is, the injection pump 8 transmits air pressure into the cavity 5, the volume of the cavity 5 is increased, the S surface is obviously raised, the support column 3 is subjected to an outward force F, the stretching force on the film is increased, the cell tissue layer 1 is subjected to stretching force (initial pretightening force and post-applied stretching force) and is in a 'stretching' state, and the length of the cell tissue layer is S 1
The pressure of the air in the cavity of the soft driver under reduced pressure is reduced, as shown in fig. 4, namely the air is pumped out of the cavity by the injection pump, the volume of the cavity is reduced, and the S surface is obviously recessed. The support column 3 is subjected to an inward force F, at which time the tensile force on the membrane becomes smaller and the cell tissue layer 1 is subjected to a compressive force in a "compressed" state with a cell tissue layer length S 2
S in FIGS. 2-4 1 >S 0 >S 2
The foregoing description is only of the preferred embodiments of the present invention, and is not intended to limit the scope of the present invention.

Claims (4)

1. A cell tissue mechanics simulation device, comprising a syringe pump (8) and a syringe pump controller (9), characterized in that: the device also comprises a soft driver (7), wherein the soft driver (7) is sequentially connected with the injection pump (8) and the injection pump controller (9);
the soft driver (7) structure comprises a cell tissue layer (1), a film (2), a support column (3), a silicon rubber layer (4), a cavity (5) and a non-extensible material layer (6); the number of the supporting columns (3) is two, and the two supporting columns are symmetrically arranged on the silicon rubber layer (4); the cell tissue layer (1) is attached to the film (2), two ends of the film (2) are fixedly arranged between the two support columns (3), and the inextensible material layer (6) is U-shaped and is arranged at the bottom of the silicon rubber layer (4);
the support column (3) and the silicon rubber layer (4) are both formed by injection molding of silicon rubber; the film (2) is an elastomer.
2. A cellular tissue mechanics simulation apparatus according to claim 1, wherein: the film (2) and the support column (3) are glued and fixed.
3. A device according to claim 1 or 2, characterized in that: the film (2) is a silica gel film.
4. A cellular tissue mechanics simulation device according to claim 3, wherein: the inextensible material layer (6) is made of fiber reinforced silica gel.
CN202010535902.0A 2020-06-12 2020-06-12 Cell tissue mechanics simulator Active CN111718835B (en)

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Publication number Priority date Publication date Assignee Title
CN113073054A (en) * 2021-04-02 2021-07-06 陕西科技大学 Cell culture device capable of providing cyclic tensile stress stimulation and manufacturing method
CN113214991B (en) * 2021-05-28 2022-12-09 西安交通大学 Cell culture device for simulating cell mechanics microenvironment

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CN2818484Y (en) * 2005-07-15 2006-09-20 中国人民解放军第四军医大学 Cell tractive tension controller
CN1932511A (en) * 2006-09-22 2007-03-21 重庆大学 Sinusoidal tensile cell loader
CN201908092U (en) * 2010-11-08 2011-07-27 北京大学口腔医院 Cell cyclic compression and tension device
CN103805511A (en) * 2014-02-18 2014-05-21 国家纳米科学中心 Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective
CN107988067A (en) * 2017-11-08 2018-05-04 西安外事学院 A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape

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WO2004094586A2 (en) * 2003-04-18 2004-11-04 Carnegie Mellon University Three-dimentional, flexible cell growth substrate and related methods
US7822457B2 (en) * 2003-11-25 2010-10-26 General Electric Company Compression paddle membrane and tensioning apparatus for compressing tissue for medical imaging purposes

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Publication number Priority date Publication date Assignee Title
CN2818484Y (en) * 2005-07-15 2006-09-20 中国人民解放军第四军医大学 Cell tractive tension controller
CN1932511A (en) * 2006-09-22 2007-03-21 重庆大学 Sinusoidal tensile cell loader
CN201908092U (en) * 2010-11-08 2011-07-27 北京大学口腔医院 Cell cyclic compression and tension device
CN103805511A (en) * 2014-02-18 2014-05-21 国家纳米科学中心 Artery blood vessel simulation microfluid control device enabling direct observation under high-power objective
CN107988067A (en) * 2017-11-08 2018-05-04 西安外事学院 A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape

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张腊全 ; 张婷 ; 贾帅军 ; 刘建 ; 熊卓 ; .定向结构组织工程软骨支架的构建.航天医学与医学工程.2012,(第03期),第212-216页. *

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