CN107988067A - A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape - Google Patents
A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape Download PDFInfo
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Abstract
A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape provided by the invention, first clamping plate and second clamping plate including placement parallel to each other, the three-dimensional tissue's module or three-dimensional tissue's module array of parcel cell are provided between first clamping plate and second clamping plate, three-dimensional tissue's module of the parcel cell is the geometric configuration with mechanical gradient, meanwhile the three-dimensional tissue's module for wrapping up cell is fixedly connected with first clamping plate and second clamping plate respectively；First clamping plate and second clamping plate are flexibly connected；The distance between two clamping plates of adjustment, realize the mechanical stretch or compression effectiveness of three-dimensional tissue's module, so that three-dimensional tissue's module is deformed after holding under tension or compression, and is being internally formed gradient strain, so as to influence cell behavior；Pass through the Array Design of loading device, you can form a kind of high-throughout three-dimensional cell mechanical gradient loading device and experiment porch；Mechanical loading experiment porch of the present invention, has the advantages that cost is low, it is simple to prepare, easy to use, size is controllable, mechanical gradient is adjustable.
Description
Technical field
The present invention relates to cell and tissue mechanical stimulation study technical field, and in particular to a kind of based on tissue given shape
Three-dimensional cell gradient mechanics loading experiment platform.
Background technology
In the cell micro-environment that body cell is in complicated, the dynamic equilibrium of microenvironment is to maintain cell normal behaviour function
Essential condition.Mechanical stimulation in microenvironment plays important regulating and controlling effect to cell behavior, participates in adjusting development, inflammation, wound
The behavior such as propagation, migration and differentiation of different cells in healing and cancer disease process.More and more researchs are found, in microenvironment
A variety of mechanical signals are not always homogeneous, are the behavior that there is and participate in regulating cell with gradient profile in many cases,
The effect of complexity is played in control body development and regeneration.Therefore, the structure of cell gradient mechanics microenvironment and its to thin
The regulating and controlling effect research of born of the same parents has become research hotspot and difficult point in cell biology.
Hydrogel is the ideal material of analog cell epimatrix two-dimensional/three-dimensional organization bracket.Advanced minute manufacturing technology
Be widely used in supporting or wrap up cell biomim betatic synthesis and microenvironment personalized customization research in.For
The softer histoorgan such as heart, muscle, connective tissue, lung, bladder, stretching mechanical stimulate relatively conventional.External structure cell
Tensile stress/strain microenvironment, research different stretch stress/strain stimulate the influence to cell behavior very necessary.At present absolutely
Most of constructive system mainly by carry cell substrate (such as hydrogel, macromolecule membrane, PDMS) carry out tensile loads,
And then the stress/strain for applying homogeneous distribution to cell stimulates, but under physio-pathological condition, body cell experience stress/
Strain stimulates often distribution gradient.But existing external Mechanical loading system is all to carry out mechanics to the cell of two dimension culture to add
Carry.Under two-dimentional cell culture system with it is larger to the response difference of mechanical stimulation in body cell, it is impossible to three-dimensional state in antimer
The real case of cell.Therefore, triaxiality/strain mechanics loading system that exploitation is simple, easily realizes is given birth to for external structure
The behavioral function of cell has important meaning under reason pathological cells stress/strain microenvironment and the internal gradient mechanical stimulation of research
Justice.
In recent years, some gradient microenvironment construction methods based on hydrogel, including microfluidic method [1], light figure are developed
Case method [2] and optical cross-linking method [3] etc..And it is that designing some makes adding for cytomorphosis for the experimental study key of cyto-mechanics
Carry and put and loading method.Existing bioreactor and Mechanical loading equipment and instrument is mainly adjusted by the width of mechanical stress
Degree, frequency, duration and dynamics etc. are limited to realize Mechanical loading in the presence of more complicated and somewhat expensive etc. is operated
Property.In addition, these instruments are only capable of realizing uniform mechanical stimulation, the mechanical gradient around body tissue can not be simulated.Thus urgently
More easy and effective, reliable new mechanics loading method need to be established.
In conclusion the relation of research gradient mechanical stimulation and cell, it will help we understand mechanics microenvironment at certain
Effect in the pathogenesis and processes of wound repair of a little diseases.Therefore, development cost is low, prepares simple, easy to use, size
Controllable, the adjustable high throughput three-dimensional cell mechanics mechanical load platform of mechanical gradient becomes functional organization's engineering and regenerative medicine
Developing goal.
The content of the invention
It is an object of the invention to provide a kind of three-dimensional cell gradient mechanics loading experiment based on tissue given shape to put down
Platform, solves existing bioreactor and Mechanical loading equipment and instrument and is mainly adjusted by the amplitude of mechanical stress, frequency, holds
Continuous time and dynamics etc. realize the Mechanical loading experiment of dimension cell gradient, and these instruments are only capable of realizing uniform mechanical stimulation,
The defects of mechanical gradient around body tissue can not be simulated.
In order to achieve the above object, the technical solution adopted by the present invention is：
A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape provided by the invention, including it is mutual
The first clamping plate and second clamping plate of parallel placement, are provided with the three-dimensional tissue of parcel cell between first clamping plate and second clamping plate
Module or three-dimensional tissue's module array, three-dimensional tissue's module of the parcel cell is the geometric configuration with mechanical gradient, together
When, the three-dimensional tissue's module for wrapping up cell is fixedly connected with first clamping plate and second clamping plate respectively；First clamping plate and second clamping plate
It is flexibly connected.
Preferably, the three-dimensional tissue's module for wrapping up cell is to prepare gained by biomaterial and mixing with cells, wherein, biology
Material uses gel or porous support materials.
Preferably, the shape for wrapping up three-dimensional tissue's module of cell is round-like structure or halfpace shape structure.
Preferably, the three-dimensional tissue's module for wrapping up cell is connected between first clamping plate and second clamping plate by 502 glue respectively
Connect.
Preferably, first clamping plate and second clamping plate be obtain having by organic or inorganic material manufacture it is identical specific several
What configuration, is the planar graph for drawing two clamping plates with CorelDRAW in computer, then X-Y scheme is passed through laser cutting machine
Material is processed into required configuration.
Preferably, first clamping plate and second clamping plate are placed in parallel up and down, and are connected between the two by four threaded posts, often
Four nuts are threaded with a threaded post, four nuts are respectively the first nut, the second nut, the 3rd nut and the 4th spiral shell
Cap, wherein, the first nut is placed in the outside of first clamping plate, the 4th nut is placed in the outside of second clamping plate, the second nut and the 3rd
Nut is placed between two clamping plates.
Preferably, the first one-dimensional movement platform and the second one-dimensional movement platform, the first one-dimensional movement platform and are further included
Two one-dimensional movement platforms are separately positioned on the both ends of two clamping plates；
Two control folders are both provided with first one-dimensional movement platform and the second one-dimensional movement platform；First clamping plate both ends
End is respectively by controlling folder and the first motor and the second motor；The end at second clamping plate both ends is respectively by controlling folder and the 3rd
Motor and the connection of the 4th motor.
Preferably, the edge for wrapping up three-dimensional tissue's module of cell is straight line, curve, waveform or convex.
Preferably, three-dimensional tissue's module array is additionally provided between first clamping plate and second clamping plate
Preferably, a length of 20~50mm, the width of two clamping plates be 10~50mm, thickness be 0.1~15mm；Described
The radius of the upper surface of three-dimensional tissue's module of round table-like parcel cell is 2.5~10mm, 5~15mm of radius of lower surface,
A height of 5~20mm.
Compared with prior art, the beneficial effects of the invention are as follows：
A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape provided by the invention, will have
The three-dimensional tissue's module or three-dimensional tissue's module array of the parcel cell of mechanical gradient geometric configuration are fixed between two clamping plates,
Meanwhile by adjusting the distance between two clamping plates, realize the mechanical stretch of three-dimensional tissue's module or three-dimensional tissue's module array
Or compression effectiveness, so that three-dimensional tissue's module of parcel cell or three-dimensional tissue's module array produce after holding under tension or compression
Change shape, gradient strain is being internally formed, so as to influence cell behavior；Pass through the Array Design of loading device, you can form one
The high-throughout three-dimensional cell mechanical gradient loading device of kind and experiment porch；Mechanical loading experiment porch of the present invention, tool
Have the advantages that cost is low, it is simple to prepare, easy to use, size is controllable, mechanical gradient is adjustable.
Further, the three-dimensional tissue's modular shape for wrapping up cell is designed as round-like structure or halfpace shape structure, made
Corresponding stress gradient, and the stress produced can be produced in portion by simple exterior stretching and compression within the organization
Size is more accurate, easy to us directly with the modeling in the real stressing conditions of body tissue.
Further, mechanical loading unit is connected with the three-dimensional tissue's module contact position for wrapping up in cell by 502 glue
Connect, on the one hand can prevent slip of the three-dimensional cell Mechanical loading module after stress from coming off, on the other hand can also be easy to mechanics
Stretching.
Further, harder organic or inorganic material is cut by simple devices such as laser cutting machines, such as by poly- diformazan
Radical siloxane (PDMS) or organic glass (PMMA) cut out the mould consistent with the geometric configuration, without multiple
Miscellaneous, expensive technology.
Further, by the cooperation of threaded post and nut, the displacement between two clamping plates is adjusted, overcomes traditional mechanics
The defects of loading equipemtn is complicated, costly；Meanwhile nut is outwards rotated mechanical static stretch effect can be achieved, will
Inwardly mechanical static compress effect, and then the strain of i.e. adjustable stretching or compression can be achieved in rotation to nut, different to adapt to
Requirement of experiment.
Further,, can be with by using easy motor, one-dimensional movement platform and clamping plate except static loading
The dynamic mechanical loading of realization device.
Further, by setting three-dimensional tissue's module array between two clamping plates, so as to fulfill high-throughout mechanics
Gradient.
Brief description of the drawings
Fig. 1 a are the structure diagrams of Mechanical loading experiment porch of the present invention；
Fig. 1 b are the structural side views of Mechanical loading experiment porch of the present invention；
Fig. 2 a are round table-like three-dimensional cell mechanical stretch gradient loading schematic diagrames prepared by embodiment 1；
Fig. 2 b are the curve maps that stretch gradient loading internal stress is distributed along frustum cone height；
Fig. 3 is round table-like three-dimensional cell mechanics compression gradient loading schematic diagram prepared by embodiment 1；
Fig. 4 is round table-like three-dimensional cell mechanical gradient dynamic load platform structure schematic diagram prepared by embodiment 2；
Fig. 5 is that embodiment 3 prepares the round table-like three-dimensional cell mechanical gradient static loading platform structure schematic diagram of high throughput；
Fig. 6 is the round table-like three-dimensional cell molded tissue block mechanical gradient dynamic load platform of curved edge prepared by embodiment 4
Structure diagram；
Wherein, 1, first clamping plate 2, second clamping plate 3, the three-dimensional tissue's module 4 for wrapping up cell, the first one-dimensional movement are put down
Platform 5, the second one-dimensional movement platform 6, the 7, first motor 8 of control folder, the second motor 9, the 3rd motor 10, the 4th motor
A, threaded post b1, the first nut b2, the second nut b3, the 3rd nut b4, the 4th nut.
Embodiment
Below in conjunction with the accompanying drawings, the present invention is described in more detail.
As shown in Figure 1 a, 1 b, a kind of three-dimensional cell gradient force based on tissue given shape provided by the invention
Loading experiment platform, including first clamping plate 1 and second clamping plate 2 are learned, it is thin that parcel is provided between first clamping plate 1 and second clamping plate 2
The three-dimensional tissue's module 3 or three-dimensional tissue's module array of born of the same parents, wrap up three-dimensional tissue's module 3 of cell respectively with first clamping plate 1 and
Second clamping plate 2 is fixedly connected.
First clamping plate 1 and second clamping plate 2 obtain having identical geometric configuration by organic or inorganic material manufacture, first
It is the planar graph for drawing first clamping plate 1 and second clamping plate 2 with CorelDRAW in computer, then X-Y scheme is passed through into laser
Material is processed into required configuration by cutting machine.
Organic material such as dimethyl silicone polymer (PDMS) or organic glass (PMMA).
The preparation of three-dimensional tissue's module 3 of cell is wrapped up, is to be mixed biomaterial and cell first, afterwards by shape
Into mixture be injected into the mould for the specific geometric configuration that gained is prepared by organic or inorganic material, it is described specific several
The mould of what configuration is the geometric configuration for having mechanical gradient.
The geometric configuration of the mechanical gradient is round-like structure or halfpace shape structure.
The shape for wrapping up three-dimensional tissue's module 3 of cell is the geometric configuration with mechanical gradient, is preferably round table-like knot
Structure or halfpace shape structure.
Three-dimensional tissue's module 3 of parcel cell passes through 502 gemel connections between first clamping plate 1 and second clamping plate 2 respectively.
One-to-one four of position is offered on two clamping plates respectively by hole, first clamping plate 1 and second clamping plate are about 2
It is placed in parallel, and between the two by four threaded post a connections, four nuts, four spiral shells is threaded with each threaded post a
Cap is respectively the first nut b1, the second nut b2, the 3rd nut b3 and the 4th nut b4, wherein, the first nut b1 is placed in first
The outside of clamping plate 1, the 4th nut b4 are placed in the outside of second clamping plate 2, and the second nut b2 and the 3rd nut b3 are placed in two clamping plates
Between.
In order to realize that the dynamic mechanical of the device loads, further include the first one-dimensional movement platform 4 and the second one-dimensional movement is put down
Platform 5, the first one-dimensional movement platform 4 and the second one-dimensional movement platform 5 are separately positioned on the both ends of two clamping plates.
Two control folders 6 are both provided with first one-dimensional movement platform 4 and the second one-dimensional movement platform 5；Two clamping plates two
The end at end is respectively by controlling folder 6 to be connected with the first motor 7, the second motor 8, the 3rd motor 9 and the 4th motor 10.
A length of 20~50mm, the width of two clamping plates are 10~50mm, thickness is 0.1~15mm.
The radius of the upper surface of round table-like three-dimensional tissue's module 3 for 2.5~10mm, lower surface radius 5~
15mm, a height of 5~20mm.
Three-dimensional cell gradient mechanics loading experiment platform of the present invention based on tissue, micro-assembly robot given shape, tool
Have the advantages that cost is low, it is simple to prepare, easy to use, size is controllable, mechanical gradient is adjustable.Simply set by laser cutting machine etc.
It is standby to cut harder organic or inorganic material, such as dimethyl silicone polymer (PDMS) or organic glass (PMMA) are cut out and
The consistent mould of the geometric configuration, without complicated, expensive technology；By on PDMS or PMMA stents, structure
Through hole is built, the movement using paired nut inside and outside threaded post and clamping plate is the displacement that can control between two clamping plates, overcomes biography
The defects of Mechanical loading equipment operation of uniting is complicated, costly；By 502 glue to mechanical loading unit and the three-dimensional group for wrapping up in cell
Knit module contact position to be attached, on the one hand can prevent slip of the three-dimensional cell Mechanical loading module after stress from coming off, separately
On the one hand mechanical stretch can also be easy to；By connect two clamping plates threaded post and nut apparatus, by the nut on clamping plate to
Mechanical static stretch effect can be achieved in outer rotation, and by the nut on clamping plate, inwardly mechanical static compress effect can be achieved in rotation；It is logical
Overregulate the displacement of two clamping plates, you can the strain of stretching or compression is adjusted, to adapt to different requirement of experiment.
, can be with realization device by using easy motor, one-dimensional movement platform and clamping plate except static loading
Dynamic mechanical loads.Pass through the Array Design of loading device, you can form a kind of high-throughout three-dimensional cell mechanical gradient loading
Device and experiment porch.Since the base materials such as computer graphics, the organic and inorganic material of laser cutting have stronger flexibility, can incite somebody to action
Loading device cuts into arbitrary shape, you can forms a kind of adjustable three-dimensional cell mechanical gradient loading device of mechanical gradient and reality
Test platform.
Embodiment 1
With reference to Fig. 1 a and Fig. 1 b, with two rectangles of computer design (a length of 25mm of the rectangle, width 15mm, thickness 5mm)
As the upper lower clamp plate of three-dimensional cell gradient Mechanical loading mould, which is cut to obtain by organic material PMMA,
Four through holes consistent with threaded post b diameters are respectively got at the both ends of two PMMA clamping plates with laser cutting machine, threaded post b's is straight
Footpath is 1.5mm.
Three-dimensional tissue's module 3 of the parcel cell of parcel cell is accompanied between two clamping plates, wraps up the three-dimensional tissue of cell
502 glue stickings of the contact site of module 3 and two clamping plates.Two clamping plate configured in parallel, the both ends of two clamping plates are equipped with through hole,
A diameter of 1.5mm, corresponding two through holes in position pass through a threaded post a on two clamping plates, in the outside screw of first clamping plate 1
The first nut b1 is connected with, the 4th nut b4 is connected with the outside screw of second clamping plate, is provided between two clamping plates
Two nut b2 and the 3rd nut b3.
In 1 structure of embodiment, mobile four the first nut b1 and four the second nut b2, displacement 2mm are rotated up.Together
When be rotated down mobile four the 3rd nut b3 and the 4th nut b4, displacement 2mm, as shown in Figure 2 a, wrap up the three-dimensional group of cell
Knit module 3 and produce gradient stretcher strain.Internal strain scatter chart is as shown in Figure 2 b, the results showed that wraps up the three-dimensional group of cell
Knit inside module 3 that there are tensile stress gradient, it was demonstrated that the device can produce elongation strain gradient；
In 1 structure of embodiment, mobile four the first nut b1 and four the second nut b2, displacement 1mm are rotated down.Together
When rotate up mobile four the 3rd nut b3 and the 4th nut b4, displacement 1mm, as shown in figure 3, the three-dimensional tissue of parcel cell
Module 3 produces gradient compression, and inside produces corresponding Strain Distribution, the results showed that wraps up in three-dimensional tissue's module 3 of cell
There are compression stress gradient in portion, it was demonstrated that the device can produce compression strain gradient.
Embodiment 2
With reference to Fig. 4, what a kind of round table-like three-dimensional cell mechanical gradient dynamic load platform, including two clamping plates 1,2 were formed
Mould, three-dimensional tissue's module 3 of the parcel cell of parcel cell is accompanied between two clamping plates 1,2, wraps up the three-dimensional tissue of cell
502 gemel connections of the contact site of module 3 and clamping plate 1,2, the both ends of two clamping plates 1,2 respectively and be located at one-dimensional movement platform 4,
On 5 control folder 6 connection, four control folder 6 respectively with the first motor 8, the second motor 9, the 3rd motor 10 and the 4th motor 11
Connection.Four motors can control to be moved on one-dimensional movement platform using computer, press from both sides 6 by four controls passes to two by displacement
A clamping plate 1,2, produces strain gradient.
Embodiment 3
With reference to Fig. 5, a kind of round table-like three-dimensional cell mechanical gradient static loading platform of high throughput, two clamping plates 1,2 are formed
Mechanical loading mould, three-dimensional tissue's module array of parcel cell, three-dimensional tissue's module array and clamping plate are accompanied between clamping plate
Contact site by 502 gemel connections, the shift length of clamping plate is controlled by the position of regulating nut, so that high throughput landform
Into the mechanical gradient of three-dimensional tissue's module array.
Embodiment 4
With reference to Fig. 6, a kind of round table-like three-dimensional cell molded tissue block mechanical gradient static loading platform of curved edge, two folders
The Mechanical loading mould that plate 1,2 is formed, round table-like, parcel cell three-dimensional tissue's module of curved edge is accompanied between clamping plate,
The contact site of three-dimensional tissue's module and clamping plate controls the displacement of clamping plate by the position of regulating nut by 502 gemel connections
Distance, so as to form strain gradient in three-dimensional tissue inside modules, and the three-dimensional cell of given shape circle edge of table except for
Straight line, extra curvature, can also be waveform or convex etc..
The present invention operation principle be：Illustrated with the structure of embodiment 1, by travelling nut adjust and control two
Displacement between clamping plate, when two clamping plates 1,2 move laterally respectively, realizes mechanical stretch effect；By two clamping plates respectively inwards
Side is moved, and realizes mechanics compression effectiveness, due to the three-dimensional group of the given shape parcel cell of the parcel cell between two clamping plates 1,2
Knit after module 3 bears displacement and be deformed, gradient strain is being internally formed, so as to influence cell behavior.
This patent mainly changes the shape of cell culture load-on module by designing, and stretches and is compressed just simply by outside
Corresponding stress gradient can be produced in portion within the organization, and the stress intensity produced is more accurate, easy to us directly with the model
Simulation is in the real stressing conditions of body tissue.If, it is necessary to adjust mechanical gradient in the range of certain size, we only need to change
Become the parameter of our cell modules, and exterior weighted platform it is not necessary to modify.
Above the embodiment only expresses the several embodiments of the present invention, its description is more specific and detailed, but not
Therefore the limitation to the scope of the claims of the present invention can be interpreted as.It should be pointed out that come for those of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.
Claims (10)
- A kind of 1. three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape, it is characterised in that：Including mutual The first clamping plate (1) and second clamping plate (2) being placed in parallel, parcel cell is provided between first clamping plate (1) and second clamping plate (2) Three-dimensional tissue's module (3), it is described parcel cell three-dimensional tissue's module (3) be the geometric configuration with mechanical gradient, meanwhile, Three-dimensional tissue's module (3) of parcel cell is fixedly connected with first clamping plate (1) and second clamping plate (2) respectively；First clamping plate (1) and Second clamping plate (2) is flexibly connected.
- 2. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 1, It is characterized in that：Three-dimensional tissue's module (3) of parcel cell is to prepare gained by biomaterial and mixing with cells, wherein, biology Material uses gel or porous support materials.
- 3. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 1, It is characterized in that：The shape for wrapping up three-dimensional tissue's module (3) of cell is round-like structure or halfpace shape structure.
- 4. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 1, It is characterized in that：Three-dimensional tissue's module (3) of parcel cell passes through 502 between first clamping plate (1) and second clamping plate (2) respectively Gemel connection.
- 5. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 1, It is characterized in that：First clamping plate (1) and second clamping plate (2) be obtain having by organic or inorganic material manufacture it is identical specific Geometric configuration, is the planar graph for drawing two clamping plates with CorelDRAW in computer, then X-Y scheme is passed through laser cutting Material is processed into required configuration by machine.
- 6. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 1, It is characterized in that：First clamping plate (1) and second clamping plate (2) are placed in parallel up and down, and are connected between the two by four threaded posts (a) Connect, be threaded with four nuts on each threaded post (a), four nuts be respectively the first nut (b1), the second nut (b2), 3rd nut (b3) and the 4th nut (b4), wherein, the first nut (b1) is placed in the outside of first clamping plate (1), the 4th nut (b4) outside of second clamping plate (2) is placed in, the second nut (b2) and the 3rd nut (b3) are placed between two clamping plates.
- 7. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 1, It is characterized in that：Further include the first one-dimensional movement platform (4) and the second one-dimensional movement platform (5), the first one-dimensional movement platform (4) The both ends of two clamping plates are separately positioned on the second one-dimensional movement platform (5)；Two control folders (6) are both provided with first one-dimensional movement platform (4) and the second one-dimensional movement platform (5)；First clamping plate (1) end at both ends is respectively by controlling folder (6) and the first motor (8) and the second motor (9)；The end at second clamping plate (2) both ends Portion is respectively by controlling folder (6) to be connected with the 3rd motor (10) and the 4th motor (11).
- It is 8. real according to a kind of three-dimensional cell gradient Mechanical loading based on tissue given shape of claim 1-7 any one of them Test platform, it is characterised in that：The edge for wrapping up three-dimensional tissue's module (3) of cell is straight line, curve, waveform or convex.
- 9. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 8, It is characterized in that：Three-dimensional tissue's module array is additionally provided between first clamping plate (1) and second clamping plate (2).
- 10. a kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape according to claim 9, It is characterized in that：A length of 20~50mm, the width of two clamping plates are 10~50mm, thickness is 0.1~15mm；The round platform The radius of the upper surface of three-dimensional tissue's module (3) of the parcel cell of shape is 2.5~10mm, 5~15mm of radius, the height of lower surface For 5~20mm.
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| CN110484426A (en) * | 2019-09-16 | 2019-11-22 | 常州市第一人民医院 | A kind of power stimulation loading device and its working method |
| CN110954249A (en) * | 2019-12-18 | 2020-04-03 | 东南大学 | Method for measuring in-vitro myocardial tissue contractility based on protein wire spring |
| CN111057640A (en) * | 2019-12-09 | 2020-04-24 | 西北工业大学 | Experimental device for in-situ research cell mechanical characteristics |
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