CN205188307U - Mechanical environment's cell culture device is chewed in simulation oral cavity - Google Patents
Mechanical environment's cell culture device is chewed in simulation oral cavity Download PDFInfo
- Publication number
- CN205188307U CN205188307U CN201520948960.0U CN201520948960U CN205188307U CN 205188307 U CN205188307 U CN 205188307U CN 201520948960 U CN201520948960 U CN 201520948960U CN 205188307 U CN205188307 U CN 205188307U
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- cell culture
- orifice plate
- loading head
- loading
- mouthsimulator
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Abstract
The utility model discloses a mechanical environment's cell culture device is chewed in simulation oral cavity, including cell culture unit, loading unit and drive unit, the cell culture unit includes that one or more establishes the cell culture hole on the orifice plate, the loading unit includes that one or more establishes the loading head of covering at the orifice plate to answer with the cell culture pore pair on the orifice plate, drive unit includes the transfer line of being reciprocating motion along orifice plate lid vertical direction, one end and the orifice plate lid directness or the indirect contact of transfer line, be equipped with the reset spring who is used for making the loading head reset between orifice plate and the orifice plate lid, the loading head is followed the cell culture hole and is up -and -down motion under transfer line and reset spring's effect. The device can the analog study the masticatory force and the different parodontium cell proliferation differentiation circumstances of chewing under the frequency of equidimension.
Description
Technical field
The utility model relates to cell culture apparatus, is specifically related to a kind of cell culture apparatus of Mouthsimulator mechanical environment.
Background technology
Under traditional cells in vitro two dimension training method and afterburning condition, scholars can apply the tension force in different size, frequency, cycle easily to monolayer cell, observe the propagation and differentiation that detect cell.But two-dimentional cell cultures can not react the growth of cell under truth in human body and intercellular contact, cell often dedifferentes, lose normal form and function; Moreover from the angle of vitro tissue regeneration, the result that two-dimentional reinforcing draws is great directive significance not.
Under dimensional culture state, the morphology and function of cell more taps in body, but the research of organization internal mechanical force has suitable complicacy.On the one hand, be difficult to separately study certain strength single to the effect of cell.Even very simple pressure is applied on a three-dimensional elastic support material, at organization internal, cell can be subject to local strain stress three kinds of power of hydrostaticpressure, hydrodynamicpressure and support, and strength size and form change different and different with space in time.On the other hand, in body, Biological Strength microenvironment feature is exactly change in time with different different with space.So, also the effect of power various in microenvironment in body need not can not be isolated and come, go as method that is afterburning with two-dimentional cell and that measure the effect studying a certain certain force respectively.
In the reinforcing research of three-dimensional, most of document is the overall stressed reaction of tissue visualization, and report proves that reinforcing promotes cell proliferation and differentiation in whole tissue.Except internal stress distribution is organized in the three-dimensional finite element model analysis done except some computers, seldom have document to be deep into difference that organization internal goes observation analysis different sites cell proliferation and differentiation.
Dental cement-periodontium in periodontal tissue-alveolar bone sample complex body is the system of a running balance, and the key maintaining this running balance is exactly the motion of physiological chewing cycle.When disease, damage or periodontal tissue are subject to external stimulus, the stem cell energy directional migration in periodontium, propagation and differentiation and regeneration and reparation periodontal tissue complex body.For the ease of the motion of in vitro study chewing cycle, be badly in need of designing and making and meet human oral cavity microenvironment feature, the device being applicable to cell in-vitro growth, reinforcing and observing.
Summary of the invention
For solving the problem, the utility model provides a kind of cell culture apparatus of Mouthsimulator mechanical environment, for the masticatory movement of Mouthsimulator, the proliferation and differentiation situation of the cell of periodontal tissue under masticatory force effect in modeling effort oral cavity, can periodontal ligament cell proliferation and differentiation situation under the masticatory force of the different size of modeling effort and different chewing frequency.
Above-mentioned purpose is achieved by the following technical solution:
Mouthsimulator chews a cell culture apparatus for mechanical environment, comprises cell culture unit, loading unit and driver element; Described cell culture unit comprises one or more cell culture well be located on orifice plate; Described loading unit comprises one or more loading head being located at orifice plate and covering, and corresponding with the cell culture well on orifice plate; Described driver element comprises the transmission rod moved reciprocatingly along orifice plate lid vertical direction, and one end of transmission rod directly or indirectly contacts with orifice plate lid; Be provided with the return spring for making loading head reset between described orifice plate and orifice plate lid, loading head pumps along cell culture well under the effect of transmission rod and return spring.
Further, be provided with multiple tail rod between described orifice plate and orifice plate lid, on the guide bar, orifice plate cover rim tail rod pumps reseting spring sleeve.Tail rod is set, orifice plate cover rim can be made tail rod and pump, be not easy to occur motion excursion, be easier to control.
Further, one end of described tail rod adjacent pores plate lid is provided with screw thread, and is provided with the supporting nut for ultimate range between regulating orifice plate lid and orifice plate.By setting nut, can ultimate range as required between regulating orifice plate lid and orifice plate, handiness is high.
Further, described loading head is removably mounted on orifice plate and covers.Loading head can need to be designed to different shapes according to test, and can need to select install or change difform loading head according to test, test handiness is high.
Further, described loading head is threaded connection and is removably mounted on orifice plate lid.Be threaded and easily change on the one hand, another convenient connection is comparatively firm, loading head can be made firmly to be arranged on orifice plate and cover.
Further, the external diameter of described loading head is less than the internal diameter of cell culture well, and loading head does open pumping along cell culture well under the effect of transmission rod and return spring.For solid medium, in loading procedure, substratum can not overflow cell culture well, and the internal diameter that the external diameter of loading head is less than cell culture well can provide loading force.
Further, the dimensional culture support of vesicular structure is provided with in described cell culture well, dimensional culture support is provided with the loading hole of agreeing with loading head shape, and loading head does open pumping to the loading hole on dimensional culture support under the effect of transmission rod and return spring.Substratum is had in the dimensional culture support of vesicular structure, Growth of Cells in the medium, loading head by loading-resetting in the loading hole of dimensional culture support, and the mechanical environment that energy Mouthsimulator is chewed, is convenient to the reproduction differentiation situation of cell in this mechanical environment of observing in dimensional culture support.
Further, described loading head outer wall is provided with the gasket of flexible material, and loading head is by gasket and cell culture well inwall close contact, and loading head does enclosed pumping along cell culture well under the effect of transmission rod and return spring.When needs directly load liquid culture matrix, in order to produce pressure (simulation loading power) in the chamber closed, with gasket by the fill gaps between loading head and cell culture well inwall, can cell reproduction differentiation situation at this pressure in modeling effort liquid culture matrix.
Further, described transmission rod lower end is fixed with transmitting spring, is contacted with orifice plate lid by transmitting spring.Transmitting spring can provide a shock absorption for the transmission of transmission rod on the one hand, strengthens the flexibility that loading head loads; On the other hand, can by transmitting spring by the size of the coefficient of elasticity controlled loading head loading force of the length compressed and this spring self.
Further, described transmission rod lower end is fixed with driver plate, is contacted with orifice plate lid by driver plate.The setting of driver plate increases the contact area between transmission rod and orifice plate lid, and it is firm that transmission is more stablized.
The beneficial effects of the utility model:
The masticatory movement of the device energy Mouthsimulator that the utility model provides, the mechanical stimulation that in cell culture well, cultured cells is subject to is similar to oral cavity periodontal film and the mechanical stimulation suffered by alveolar bone.This device utilizes the in-vitro simulated feature being easy to control, be convenient to the proliferation and differentiation situation of cell under masticatory force effect of periodontal tissue in modeling effort oral cavity, can periodontal ligament cell proliferation and differentiation situation under the masticatory force of the different size of modeling effort and different chewing frequency.
Accompanying drawing explanation
Fig. 1 is loading unit and the structural representation cultivating unit;
Fig. 2 is a kind of structural representation (open loading) of loading head and culture hole;
Fig. 3 is a kind of structural representation (closed loading) of loading head and culture hole;
Fig. 4 is that transmission rod is contacted with orifice plate lid by transmitting spring;
Fig. 5 is that transmission rod is contacted with orifice plate lid by driver plate;
Fig. 6 is a kind of principle schematic of transmission rod transmission.
Wherein, 1, orifice plate; 2, orifice plate lid; 3, cell culture well; 4, loading head; 5, transmission rod; 6, return spring; 7, tail rod; 8, nut; 9, dimensional culture support; 10, gasket; 11, transmitting spring; 12, driver plate; 13, transmission rod guide rail; 14, liquid culture matrix; 15, motor output shaft; 16, support.
Embodiment
The technical solution of the utility model is described in detail below in conjunction with specific embodiment.
A kind of Mouthsimulator as shown in Figure 1 chews the cell culture apparatus of mechanical environment, comprises cell culture unit, loading unit and driver element.Described cell culture unit comprises one or more cell culture well 3, and cell culture well 3 is located on orifice plate 1.Described loading unit comprises one or more loading head 4, and loading head 4 is located on orifice plate lid 2, and corresponding with the cell culture well 3 on orifice plate 1.Described driver element comprises the transmission rod 5 moved reciprocatingly along orifice plate lid 2 vertical direction, and one end of transmission rod 5 directly or indirectly contacts (as shown in Figures 4 and 5) with orifice plate lid 2.Be provided with the return spring 6 for making loading head 4 reset between described orifice plate 1 and orifice plate lid 2, loading head 4 pumps along cell culture well 3 under the effect of transmission rod 5 and return spring 6.
In order to make pumping of orifice plate lid 2 not occur skew, be provided with multiple tail rod 7 between described orifice plate 1 and orifice plate lid 2, return spring 6 is enclosed within tail rod 7, and orifice plate lid 2 pumps along tail rod 7, as shown in Figure 1.Tail rod 7 is set, orifice plate lid 2 can be made to pump along tail rod 7, be not easy to occur motion excursion, be easier to control.
For the ease of the ultimate range between regulating orifice plate lid 2 and orifice plate 1, one end of described tail rod 7 adjacent pores plate lid 2 is provided with screw thread, and is provided with the supporting nut 8 for ultimate range between regulating orifice plate lid 2 and orifice plate 1.By setting nut 8, can ultimate range as required between regulating orifice plate lid 2 and orifice plate 1, handiness is high.
In order to improve the utilization ratio of orifice plate lid 2, simultaneously for the ease of selecting the loading head 4 changing different shapes and type, described loading head 4 is removably mounted on orifice plate lid 2.Loading head can need to be designed to different shapes according to test, and can need to select install or change difform loading head according to test, test handiness is high.Particularly, described loading head 4 can be threaded connection and be removably mounted on orifice plate lid 2.Be threaded and easily change on the one hand, another convenient connection is comparatively firm, and loading head 4 can be made firmly to be arranged on orifice plate lid 2.
When needing solid medium or load dimensional culture support, the external diameter of described loading head 4 is less than the internal diameter of cell culture well 3, and loading head 4 does open pumping along cell culture well 3 under the effect of transmission rod 5 and return spring 6.Open pumping just refers to blow-by between loading head 4 and cell culture well 3.Specifically as shown in Figure 2, the dimensional culture support 9 of vesicular structure is provided with in cell culture well, dimensional culture support 9 is provided with the loading hole of agreeing with loading head 4 shape, and loading head 4 does open pumping to the loading hole on dimensional culture support 9 under the effect of transmission rod and return spring.Substratum is had in the dimensional culture support 9 of vesicular structure, Growth of Cells in the medium, loading head by loading-resetting in the loading hole of dimensional culture support, the mechanical environment that energy Mouthsimulator is chewed, is convenient to the reproduction differentiation situation of cell in this mechanical environment of observing in dimensional culture support.
When needing to load liquid culture matrix, as shown in Figure 3, loading head 4 outer wall is provided with the gasket 10 of flexible material, loading head 4 is by gasket 10 and cell culture well inwall close contact, and loading head 4 does enclosed pumping along cell culture well under the effect of transmission rod and return spring.Enclosed pumping just refers to and seals between loading head 4 and cell culture well 3.When loading head 4 loads, produce pressure (simulation loading power) in closed chamber, can cell reproduction differentiation situation at this pressure in modeling effort liquid culture matrix.
As shown in Figure 4, transmission rod 5 lower end can be fixed with transmitting spring 11, is contacted with orifice plate lid 2 by transmitting spring 11.Transmitting spring 11 1 aspect can think that the transmission of transmission rod 5 provides a shock absorption, strengthens the flexibility that loading head 4 loads; On the other hand, can by transmitting spring 11 by the size of the coefficient of elasticity controlled loading head loading force of the length compressed and this spring self, the cell proliferation and differentiation situation under the different loading force of convenient research.
As shown in Figure 5, transmission rod 5 lower end can be fixed with driver plate 12, is contacted with orifice plate lid 2 by driver plate 12.The setting of driver plate 12 increases the contact area between transmission rod 5 and orifice plate lid 2, and it is firm that transmission is more stablized.
Fig. 6 is a kind of principle of work schematic diagram of transmission rod, and the transmission rod 5 in figure is three bar linkage structures, and one end of three bar linkage structures is fixed on motor output shaft 15, and the other end pumps along with the rotation of motor output shaft 15 through transmission rod guide rail 13.Motor and transmission rod guide rail 13 are fixed on support 16.For the ease of regulating loading frequency, described motor can be connected with the speed regulator for controlling motor switch and rotating speed, and described speed regulator can adopt direct-current brush motor motor width modulation speed regulator.
In an example of the present utility model, the porous plate (as 48 orifice plates) adopting laboratory conventional and EP pipe are as experimental material, and EP pipe through the lid simulation loading head of porous plate, and corresponds to culture hole.Four angles place of porous plate and lid is respectively equipped with 4 springs, uses the transmission rig shown in Fig. 6 to load the lid of porous plate.EP pipe pumps under the effect of transmission rig and spring.Have dimensional culture support and cell culture fluid in culture hole, wherein, situation is broken up in the reproduction of observation of cell under loading force effect to Growth of Cells.
The masticatory movement of the device energy Mouthsimulator that the utility model provides, the mechanical stimulation that in cell culture well, cultured cells is subject to is similar to oral cavity periodontal film and the mechanical stimulation suffered by alveolar bone.This device utilizes the in-vitro simulated feature being easy to control, be convenient to the proliferation and differentiation situation of cell under masticatory force effect of periodontal tissue in modeling effort oral cavity, can periodontal ligament cell proliferation and differentiation situation under the masticatory force of the different size of modeling effort and different chewing frequency.
The effect of above-described embodiment is essentiality content of the present utility model is described, but does not limit protection domain of the present utility model with this.Those of ordinary skill in the art should be appreciated that and can modify to the technical solution of the utility model or equivalent replacement, and does not depart from essence and the protection domain of technical solutions of the utility model.
Claims (10)
1. Mouthsimulator chews a cell culture apparatus for mechanical environment, it is characterized in that: comprise cell culture unit, loading unit and driver element; Described cell culture unit comprises one or more cell culture well be located on orifice plate; Described loading unit comprises one or more loading head being located at orifice plate and covering, and corresponding with the cell culture well on orifice plate; Described driver element comprises the transmission rod moved reciprocatingly along orifice plate lid vertical direction, and one end of transmission rod directly or indirectly contacts with orifice plate lid; Be provided with the return spring for making loading head reset between described orifice plate and orifice plate lid, loading head pumps along cell culture well under the effect of transmission rod and return spring.
2. Mouthsimulator according to claim 1 chews the cell culture apparatus of mechanical environment, it is characterized in that: be provided with multiple tail rod between described orifice plate and orifice plate lid, and on the guide bar, orifice plate cover rim tail rod pumps reseting spring sleeve.
3. Mouthsimulator according to claim 2 chews the cell culture apparatus of mechanical environment, it is characterized in that: one end of described tail rod adjacent pores plate lid is provided with screw thread, and is provided with the supporting nut for ultimate range between regulating orifice plate lid and orifice plate.
4. Mouthsimulator according to claim 1 chews the cell culture apparatus of mechanical environment, it is characterized in that: described loading head is removably mounted on orifice plate and covers.
5. Mouthsimulator according to claim 4 chews the cell culture apparatus of mechanical environment, it is characterized in that: described loading head is threaded connection and is removably mounted on orifice plate lid.
6. Mouthsimulator according to claim 1 chews the cell culture apparatus of mechanical environment, it is characterized in that: the external diameter of described loading head is less than the internal diameter of cell culture well, loading head does open pumping along cell culture well under the effect of transmission rod and return spring.
7. Mouthsimulator according to claim 6 chews the cell culture apparatus of mechanical environment, it is characterized in that: the dimensional culture support being provided with vesicular structure in described cell culture well, dimensional culture support is provided with the loading hole of agreeing with loading head shape, and loading head does open pumping to the loading hole on dimensional culture support under the effect of transmission rod and return spring.
8. Mouthsimulator according to claim 1 chews the cell culture apparatus of mechanical environment, it is characterized in that: described loading head outer wall is provided with the gasket of flexible material, loading head is by gasket and cell culture well inwall close contact, and loading head does enclosed pumping along cell culture well under the effect of transmission rod and return spring.
9. Mouthsimulator according to claim 1 chews the cell culture apparatus of mechanical environment, it is characterized in that: described transmission rod lower end is fixed with transmitting spring, is contacted with orifice plate lid by transmitting spring.
10. Mouthsimulator according to claim 1 chews the cell culture apparatus of mechanical environment, it is characterized in that: described transmission rod lower end is fixed with driver plate, is contacted with orifice plate lid by driver plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520948960.0U CN205188307U (en) | 2015-11-25 | 2015-11-25 | Mechanical environment's cell culture device is chewed in simulation oral cavity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520948960.0U CN205188307U (en) | 2015-11-25 | 2015-11-25 | Mechanical environment's cell culture device is chewed in simulation oral cavity |
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| Publication Number | Publication Date |
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| CN205188307U true CN205188307U (en) | 2016-04-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520948960.0U Withdrawn - After Issue CN205188307U (en) | 2015-11-25 | 2015-11-25 | Mechanical environment's cell culture device is chewed in simulation oral cavity |
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| CN (1) | CN205188307U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105331537A (en) * | 2015-11-25 | 2016-02-17 | 季骏 | Cell culture device capable of simulating oral cavity chewing mechanical environment |
| CN106367347A (en) * | 2016-11-04 | 2017-02-01 | 大连医科大学附属第医院 | Biological support material fixed mount and cell culture method employing same |
| CN107988067A (en) * | 2017-11-08 | 2018-05-04 | 西安外事学院 | A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape |
-
2015
- 2015-11-25 CN CN201520948960.0U patent/CN205188307U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105331537A (en) * | 2015-11-25 | 2016-02-17 | 季骏 | Cell culture device capable of simulating oral cavity chewing mechanical environment |
| CN106367347A (en) * | 2016-11-04 | 2017-02-01 | 大连医科大学附属第医院 | Biological support material fixed mount and cell culture method employing same |
| CN107988067A (en) * | 2017-11-08 | 2018-05-04 | 西安外事学院 | A kind of three-dimensional cell gradient mechanics loading experiment platform based on tissue given shape |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20160427 Effective date of abandoning: 20171003 |