CN1847847A - Cell mechanical period loading system - Google Patents
Cell mechanical period loading system Download PDFInfo
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- CN1847847A CN1847847A CN 200610054278 CN200610054278A CN1847847A CN 1847847 A CN1847847 A CN 1847847A CN 200610054278 CN200610054278 CN 200610054278 CN 200610054278 A CN200610054278 A CN 200610054278A CN 1847847 A CN1847847 A CN 1847847A
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- culture chamber
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Abstract
The present invention relates to cytomechanical loading system including a culturing mechanism and a controlling mechanism. The cytomechanical loading system features its culturing mechanism comprising four independent culturing cavities with silica gel film for culturing cell in the bottom. Of the four culturing cavities, three have raising plates and raising air bags of different mechanical loads and connected via control valve to the main air bag, which has motor driven reciprocally moving pushing plate in the bottom; and the rest one has no any mechanical load. All the four culturing cavities form one mechanical load contrast set. The present invention can generate planar equal-biaxial periodical pull stress and pressure stress and quasi physiological state, and has the features of high controllability, simple operation, high repeating precision, etc.
Description
Technical field
The present invention relates to the medical biotechnology experimental apparatus, is a kind of cell mechanical period loading system that the analogue body inner cell is subjected to cycle drawing stress and cycle compression stress that is used for.
Technical background
According to biomechanics and biomedical engineering progress of research, is that the present age is international biological with cyto-mechanics method various stressed fibroblasts in bodies such as the different mechanical loading inferior ligament of in vitro study cell, cardiac muscle cell at molecule and modal variation, the focus of medical research, in order to study various stressed tissues, cell molecular level or the morphologic variation under different mechanical loadings in cardiac muscle, ligament, synovial membrane and the body, therefore, need a kind of instrument that can produce periodical pull stress or compression stress.
Cyto-mechanics is the field, forward position of modern biology development, relates to the deformation of cell under the load, cell membrane, cytoskeleton, Mechanical Properties such as elastic constant, viscoelasticity, adhesive force; And the mechanics factor pair cell sticks, sprawls and grow, breaks up or the like the influence of biological behaviour.The basis of cyto-mechanics research and key are the cell loading techniques.Because internal milieu is complicated unusually; The size of human body cell is between tens to tens microns, and the thickness of cell membrane only has several nanometers to tens nanometer, and conventional macromechanics loading method and experiment can't directly be used.Therefore, the in-vitro separation cell is the matter of utmost importance that cyto-mechanics faces with setting up suitable loading culture model.As far back as 1939, Glucksmann has carried out ground-breaking research aspect the mechanics loading development of cultured cell in vitro.He cultivates the shin bone endo cell of Embryo Gallus domesticus on paired intercostal muscle matrix, and when muscular atrophy traction rib was close mutually, the cell of cultured in vitro promptly had been subjected to the effect of pressure.American Lee owing to need not consider the periodicity effect of cell tensile and compression, can only carry out static drawing stress or compression stress test by pair cell at the twin shaft stretch devices such as static state of invention in 1996.Through improving for many years and developing, the mechanics of having developed multiple cultured cell in vitro loads.Roughly can be divided into centrifugal loading, fluid loading, unicellular loading, pressure passes to and loads and base deformation charger.
The content of invention
The object of the present invention is to provide a kind of cycle to stretch and the mechanics load apparatus of compression cell, it has near true physiological status, and controllability is good, and is simple to operate, repeatable accuracy height, characteristics such as error is little, and volume is little.
The objective of the invention is to realize by following technical proposals:
Described cell mechanical period loading system comprises cultivates mechanism and control gear, wherein, control gear comprises central processing unit 18, driving circuit 20, carry out control circuit 19 and power circuit 21, carry out control circuit 19 and comprise liquid level sensor 5 and gas channel operation valve 12, central processing unit 18 and driving circuit 20 are carried out control circuit 19 and are linked to each other; Power circuit 21 respectively with central processing unit 18, driving circuit 20 is carried out control circuit 19 and is linked to each other; Feature of the present invention is:
Cultivate mechanism by four independently culture chamber constitute, the bottom of three culture chambers is fixed with the pellosil of cultured cell respectively therein, its bottom has raising plate and the jacking type air bag that is loaded with different mechanics loads, three jacking type air bags are communicated with main gasbag by the gas channel operation valve, are equipped with bottom the main gasbag by what driven by motor moved back and forth and push plate; Another culture chamber does not load any mechanics load, and they form one group of mechanics load control group together.
Characteristics of the present invention are as follows:
(1) because the pellosil of cultured cell is circular, and around film, produces tension force, so the present invention can produce uniform the stretching and force of compression to the cell of cultivating on pellosil.
The frequency of the adjusting linear motor that (2) we can be artificial in device of the present invention, thus cause that pellosil stretches and the variation of contraction frequency.So the present invention can produce cultivating the periodicity drawing stress and the compression stress of the cell different frequency on pellosil.
(3) because type of drive of the present invention is to utilize air bag to drive the jacking piece, and adopted liquid level fiber switch sensor, make gas be convenient to accurate control, and the force rate that produces is milder, thus the present invention to cultivate the periodic stretching that produces at the cell on the pellosil and compression stress very approach power suffered under the cell physiological state.
(4) because the inflation of circuit of the present invention and gas, to die be respectively to adopt very high performance integrated circuit and fiber switch sensor.So the height reliability and the good stable of the operation of assurance system accurately.
(5) the present invention adopts the compound total system that makes of a plurality of different stretch amplitude culture systems to increase work efficiency greatly.Owing to adopted the double dish of four different mechanics loads to make total system when experimentizing, have very strong comparative, can reduce workload greatly, improved conventional efficient.
(6) volume of the present invention is less, can be placed on the cell culture incubator the inside and cultivate.
Based on above characteristics, the present invention can produce many groups, periodicity drawing stress and compression stress.Have characteristics such as controllability is good, simple to operate, repeatable accuracy is high, error is little, comparative is strong, volume is little.
Description of drawings
Below in conjunction with further detailed elaboration the present invention of accompanying drawing, but the present invention is not limited only to the accompanying drawing illustrated embodiment.
Fig. 1 is the front section view of topworks of the present invention.
Fig. 2 is the side sectional view of topworks of the present invention.
Fig. 3 is the vertical view of topworks of the present invention.
Fig. 4 is control principle figure of the present invention.
In Fig. 1～3,1-pellosil, 2-spherical head, 3-is threaded, 4-fiber switch sensor, 5-fiber switch sensor signal receiver, 6-annular jacking piece, 7-annular rubber ring, 8-ring groove, the 9-hoisting gasbag, 10-culture chamber bearing, 11-gas channel, 12-gas channel operation valve, the 13-main gasbag, 14-is threaded, 15-pushes plate, 16-main gasbag bearing, 17-linear electric motors.
Embodiment
With cellular incubation on pellosil 1, pellosil 1 is by annular rubber ring 7 being pressed in the ring groove 8 tightly, for making pellosil 1 keep a surface level, adopt a threaded spherical head 2, the screw thread of the screw thread of spherical head 2 and culture chamber bearing 10 upper ends being interlocked tightly is its being fixed on the double dish bearing tightly.
Hoisting gasbag is positioned at culture chamber bearing 10, and culture chamber bearing 10 links to each other with the screw thread of main gasbag bearing 16 upper ends by its terminal screw thread.To guarantee the integraty of total system.
Wherein, when hoisting gasbag 9 inflation, make its that volume take place and become big deformation, and, make annular jacking piece 6 do motion upwards, cause that pellosil 1 does deformation and become big extensional motion, tensile cell the thrust that annular jacking piece 6 produces.And the liquid level that causes nutrient solution on the pellosil 1 rises, when nutrient solution rises to the position of fiber switch sensor signal receiver 5, detected by fiber switch sensor 4, by gas channel operation valve 12 closed gas flow passage 11 immediately, so just having stopped the inflation of hoisting gasbag 9, and caused that annular jacking piece 6 stops to move upward. switch keeps the shut-in time by importing in the good program of beginning central processing unit controlling Design.Through this time, air communication logos and utensils 12 is made the order of opening gas channel 11 switches by the signal that central processing unit sends.
Push plate 15 by linear electric motors 17 drives of central processing unit control and produce downward motion, cause that the volume of main gasbag 13 increases, the gas in the hoisting gasbag 9 is flexible to be crossed in the gas channel 11 inflow main gasbags 13.The diminishing of hoisting gasbag 9 volumes causes that annular jacking piece 6 does downward motion, and annular jacking piece 6 is done the contractile motion that downward motion causes that again pellosil 1 deformation diminishes, the compression cell.
Wherein hoisting gasbag 9, and main gasbag 13 is to keep continuous sealing integral body by gas channel 11, does not therefore have the problem of gas leakage.Linear electric motors 17 are done the frequency that pumps and are set by central processing unit, and its frequency and gas channel 11 switches time of closing are consistent.The distance of gas channel 11 is very short, and diameter is relatively large, has so just reduced gas time delay.The size that described gas channel operation valve control gas channel opens and closes.
Among Fig. 2,18 for there not being the culture chamber of mechanics load, do not have the culture chamber 18 of mechanics load and the culture chamber of other mechanics load to organize as a comparison, forms total system.Four culture chamber systems are arranged in the total system, and wherein three are subjected to main gasbag control, and another does not add any mechanics and loads.Main gasbag 13 is controlled 3 identical hoisting gasbags 9 of shape respectively, but wherein the gas charging degree of each air bag is different, and this point is to decide by different adjustment fiber switch sensor 4, the position height of fiber switch sensor signal receiver 5 on spherical head 2.
Among Fig. 4, A, B, C, D-culture chamber, the 18-central processing unit, 19-carries out control circuit, 20-driving circuit, 21-and power circuit.The coordination of making the linear electric motors 17 that periodically move up and down in the moving system 20 of air flow switch controller system and transfer drive realizes by central processing unit 18.Power circuit 21 links to each other with central processing unit 18, the moving system 20 of transfer drive, execution control circuit 19 respectively.Provide power supply by this system power supply circuit 21 for whole device.The existing mature technology that control gear adopts is not main points of the present invention.
Material: the spherical head 2 in the total system, annular jacking piece 6, ring groove 8, culture chamber bearing 10, main gasbag bearing 16 is pushed plate 15 and is cast by polystyrene.
Claims (5)
1, a kind of cell mechanical period loading system, comprise and cultivate mechanism and control gear, wherein, control gear comprises central processing unit (18), driving circuit (20), carry out control circuit (19) and power circuit (21), carry out control circuit (19) and comprise liquid level sensor and gas channel operation valve (12); Feature of the present invention is: cultivate mechanism by four independently culture chamber constitute, be fixed with the pellosil of cultured cell respectively in the bottom of culture chamber, three bottom has raising plate and the hoisting gasbag that is loaded with different mechanics loads (9) therein, three jacking type air bags are communicated with main gasbag (13) by the gas channel operation valve, are equipped with bottom the main gasbag by what driven by motor moved back and forth and push plate; Another culture chamber does not load any mechanics load, and they form one group of mechanics load control group together.
2, cell mechanical period loading system according to claim 1, it is characterized in that: hoisting gasbag (9) lays respectively in four holes of culture chamber bearing (10), and the culture chamber bearing is by the vicinal one mutually of the screw thread on its terminal screw thread and the main gasbag bearing (16).
3, cell mechanical period loading system according to claim 1, it is characterized in that: pellosil (1) divides in other ring groove that is pressed in culture chamber bearing hole (8) by annular rubber ring (7), the screw thread that adopts a threaded spherical head (2) and culture chamber bearing (10) upper end being interlocked tightly is its being fixed on the double dish bearing and constituting culture chamber tightly.
4, according to claim 1,3 described cell mechanical period loading systems, it is characterized in that: the sidewall at culture chamber is provided with liquid level sensor.
5, cell mechanical period loading system according to claim 4 is characterized in that: liquid level sensor is made up of fiber switch sensor (5) and receiver (4) thereof, and they are arranged on the position of spherical head (2) correspondence that constitutes the culture chamber sidewall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100542782A CN100570363C (en) | 2006-05-09 | 2006-05-09 | Cell mechanical period loading system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100542782A CN100570363C (en) | 2006-05-09 | 2006-05-09 | Cell mechanical period loading system |
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| CN1847847A true CN1847847A (en) | 2006-10-18 |
| CN100570363C CN100570363C (en) | 2009-12-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2006100542782A Expired - Fee Related CN100570363C (en) | 2006-05-09 | 2006-05-09 | Cell mechanical period loading system |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475908B (en) * | 2009-01-16 | 2011-06-29 | 中国人民解放军第三军医大学 | Cultivation apparatus and method for implementing continuous tension stress action to cell |
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN105388067A (en) * | 2015-11-23 | 2016-03-09 | 中国科学院武汉岩土力学研究所 | Uniform load distribution supercharging device, and three-dimensional side slope destruction model testing apparatus and method |
| CN111423980A (en) * | 2020-04-08 | 2020-07-17 | 中国科学院力学研究所 | Fully-closed cell substrate stretching and flow shearing combined loading experiment system |
| CN113604358A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Cell loading device capable of replacing plane force and pneumatic control method |
| CN113604349A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional cell loading device using sample rack and pneumatic control method |
| US11603516B2 (en) | 2017-03-21 | 2023-03-14 | Centre National De La Recherche Scientifique | Device for mechanically characterizing an element of interest such as an oocyte |
-
2006
- 2006-05-09 CN CNB2006100542782A patent/CN100570363C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN101475908B (en) * | 2009-01-16 | 2011-06-29 | 中国人民解放军第三军医大学 | Cultivation apparatus and method for implementing continuous tension stress action to cell |
| CN105388067A (en) * | 2015-11-23 | 2016-03-09 | 中国科学院武汉岩土力学研究所 | Uniform load distribution supercharging device, and three-dimensional side slope destruction model testing apparatus and method |
| CN105388067B (en) * | 2015-11-23 | 2018-05-04 | 中国科学院武汉岩土力学研究所 | Evenly load supercharging device, three-dimensional slope damage model test device and method |
| US11603516B2 (en) | 2017-03-21 | 2023-03-14 | Centre National De La Recherche Scientifique | Device for mechanically characterizing an element of interest such as an oocyte |
| CN111423980A (en) * | 2020-04-08 | 2020-07-17 | 中国科学院力学研究所 | Fully-closed cell substrate stretching and flow shearing combined loading experiment system |
| CN111423980B (en) * | 2020-04-08 | 2022-03-15 | 中国科学院力学研究所 | Fully-closed cell substrate stretching and flow shearing combined loading experiment system |
| CN113604358A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Cell loading device capable of replacing plane force and pneumatic control method |
| CN113604349A (en) * | 2021-07-29 | 2021-11-05 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional cell loading device using sample rack and pneumatic control method |
| CN113604349B (en) * | 2021-07-29 | 2023-10-13 | 中国科学院长春光学精密机械与物理研究所 | Three-dimensional cell loading device using sample rack |
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| CN100570363C (en) | 2009-12-16 |
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Granted publication date: 20091216 Termination date: 20110509 |